CN205795900U - Dressing with cell spheroids - Google Patents
Dressing with cell spheroids Download PDFInfo
- Publication number
- CN205795900U CN205795900U CN201521060431.3U CN201521060431U CN205795900U CN 205795900 U CN205795900 U CN 205795900U CN 201521060431 U CN201521060431 U CN 201521060431U CN 205795900 U CN205795900 U CN 205795900U
- Authority
- CN
- China
- Prior art keywords
- cell
- dressing
- globoid
- layer
- wound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004027 cell Anatomy 0.000 claims description 47
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 claims description 38
- 210000000130 stem cell Anatomy 0.000 claims description 19
- 238000004132 cross linking Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000499 gel Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 239000010410 layer Substances 0.000 abstract 3
- 239000011247 coating layer Substances 0.000 abstract 1
- 230000037314 wound repair Effects 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 16
- 208000027418 Wounds and injury Diseases 0.000 description 16
- 230000035876 healing Effects 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 6
- 230000006870 function Effects 0.000 description 5
- 230000003020 moisturizing effect Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 206010072170 Skin wound Diseases 0.000 description 2
- 102000013275 Somatomedins Human genes 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000028990 Skin injury Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 210000002469 basement membrane Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000010478 bone regeneration Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 230000003631 expected effect Effects 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003757 neuroblast Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Abstract
The utility model provides a dressing with cell spheroids, which comprises a template layer, a plurality of holes are arranged on the template layerA coating layer which is cross-linked and solidified in the holes of the template layer and is embedded with 5 x 105A granular cell spheroid; therefore, the dressing can generate the effects of wound repair or skin care and the like through the dressing layer.
Description
Technical field
This utility model is relevant with the dressing of human body skin, refers in particular to a kind of dressing with cell globoid.
Background technology
Skin is the organ that human body is maximum, having the function of the external pathogenic factor of perception detecting, moisturizing and opposing, causing afunction or exception if coming to harm, it may be possible to provide pathogenic factor invasion causes systemic infection to cause disease.Many sufferers are had to subject the pain that skin injury is caused now, the large area burn and scald that causes as unexpected, the puzzlement of the acute or chronic wound such as patient of diabetes wound is difficult to healing, the decubital ulcer of long-term bed is festered.
The most extensively general dressing in commercially available wound; only prevent the defencive function that antibacterial enters and tissue fluid is oozed out; such as gauze; a little have the functional artificial synthetic dressing adding metal ion or vitamin e etc.; help to suppress the invasion of external cause of disease; but lacking similar skin natural component structure and basement membrane, therefore healing rate is slow and recovers functional difference.It addition, also have the dressing declaring to add the synthetically grown factor, but the problem whether its composition can be treated to solve by biological decomposition.Additionally, in the face of the reparation of large area wound need to be treated with skin auto or heteroplastic transplantation, but also it is encountered by the problems such as transplant rejection, the generation of new wound or people and animals' infectious disease.
The regenerative medicine of rising in recent years is that problem above brings new opportunity, stem cell is undifferentiated cell, it has the characteristic of self renewal (Self-renewal) and differentiation (Differentiation), therefore stem cell is in mankind's wounded tissue or disease, it is considered to have an opportunity to be divided into the cell with function, and carries out the curative effect repaired Yu regenerate.It is many that stem cell obtains source, such as embryonic stem cell, cord blood stem cell or bone marrow stem cell, but acquirement is difficult to and acquirement amount is restricted, more important question is that the challenge of moral ethics is faced, but develop inductivity versatile stem cell (iPS), the most still have that induced efficiency is low and genetic manipulation is complicated considers problem.But fat stem cell obtains in fatty tissue, being also pluripotent cell, compared with other stem cell, easily and obtainable cell quantity is more in acquirement.
On Wound healing and bone regeneration, research has been pointed out stem cell injection wound can shorten healing time, and data display growth factor content improves.Also having document construction cultivation fat stem cell to make cell sheet, tool is possessed cell characteristics and contributes to wound healing and cell regeneration, but technology still effect is limited at present.The above, researcher can be by the interpolation of stem cell to reach the effect of skin wound reparation or skin care, to this end, the research for stem cell has the usability in its industry with application.
Fat stem cell is then trained spheroid by TaiWan, China patent of invention I490337, is more beneficial for transdifferentiation neuroblast, it is seen that its cultural method is more beneficial for the secretion activity factor.TaiWan, China new patent M464150 is then to utilize the cultivation liquid cultivating cell to be applied in dressing, and this cultivation liquid contains most somatomedin secreted by cell, can promote that Skin Cell regenerates.
But, cell environment in vitro is difficult to long-term surviving, and the amount therefore obtaining somatomedin is limited, affects expected effect.Therefore, cell is applied in dressing also have many problems need to overcome and effort.
Utility model content
Main purpose of the present utility model is i.e. to provide a kind of dressing with cell globoid solving aforesaid drawbacks, it not only includes plural number cell globoid, more can promote the survival rate of cell globoid, and can promote that cell globoid secretes more active factors, and then the reparation of wound, healing can be accelerated.
Then, for reaching aforesaid purpose, this utility model provides a kind of dressing with cell globoid, includes a template layer, it offers some aperture；One dressing layer, if crosslinking curing is embedded with stem cell globoid inside it in the aperture of this template layer.
Further, the material of this template layer is selected from, but not limited to, silica gel, rubber.
Further, this dressing layer is gel.
Further, this cell globoid includes but not limited to fat stem cell globoid.
Further, the number of this cell globoid is 5 × 105?.
The beneficial effects of the utility model are:
This utility model provides a kind of dressing with cell globoid, its dressing layer includes the structure of plural number cell globoid, available moisturizing and the dressing layer of tool nutrient, this cell globoid is made will not quickly to reduce survival rate because of disadvantageous vitro, and can promote that cell globoid secretes more active factors, and then accelerate the reparation of wound, healing, very have practical value.
Accompanying drawing explanation
Fig. 1 is the top view of this utility model one preferred embodiment.
Fig. 2 is the sectional view in 2-2 direction along Fig. 1.
Detailed description of the invention
Hereinafter, hereby lift this utility model one preferred embodiment, and coordinate accompanying drawing to be described in further detail as rear:
Refer to shown in Fig. 1 and Fig. 2, the dressing 10 with cell globoid of this utility model one preferred embodiment, include template layer 12 and a dressing layer 14.
This template layer 12, makes with the elastic material heated baking such as silica gel or rubber disinfection, and it offers one or more than one aperture 22, and this aperture 22 mainly meets wound area, and visual wound area size adjusts.
This dressing layer 14, in gel, cultivate concentrated solution ratio uniform with sterile collagen protein liquid and the cell of 8:1 (not limiting) and mix, and pH value (does not limits) between 7.2 to 7.6, crosslinking curing is in the aperture 22 of this template layer 12, and it is internal and is embedded with 5 × 105(not limiting) cell globoid 24.This dressing layer 14 is as follows with the preparation method of cell globoid 24: previously prepared respectively this cell globoid 24, it can be fat stem cell globoid (not limiting), first take each liquid pearl containing about 1000 cell number, then liquid pearl is dropped in the form of an array (three dimensional constitution Hanging drop culture) in culture dish, in order to the maximization of cell number and longer-term can be made to survive in external, to act on reparation or the maintenance of skin wound, and then turn out respectively this cell globoid 24, the sterile collagen protein liquid adding this dressing layer 14 is mixed homogeneously with cell culture fluid, finally pour crosslinking curing in the aperture 22 of this template layer 12 into complete to combine.
Thereby, this utility model has this dressing 10 of cell globoid and is available for adhering on the wound of skin, promotes the healing of wound, and its characteristic is as follows with effect:
First, the collagen protein liquid that this dressing layer 14 includes has the basic function of moisturizing, again containing the active factors of its fat stem cell secretion, has maintenance and repairs or regeneration function.
Secondly, included the structure of plural number cell globoid 24 by this dressing layer 14, not only can improve the survival rate of cell globoid, and can promote that cell globoid secretes more active factors, and then accelerate the reparation of wound, healing.
Furthermore, the preparation of this template layer 12 is simple, and this aperture 22 can be changed according to the difference of wound size, is suitable for large area or the wound of non-specification shape.
As from the foregoing, this utility model is provided the dressing with cell globoid, its dressing layer includes the structure of plural number cell globoid, available moisturizing and the dressing layer of tool nutrient, this cell globoid is made will not quickly to reduce survival rate because of disadvantageous vitro, and can promote that cell globoid secretes more active factors, and then accelerate the reparation of wound, healing, very have practical value；Then, this utility model meets the regulation of new patent really, files an application the most in accordance with the law.
The foregoing is only a preferred embodiment of the present utility model, any those skilled in the art, impartial change and the modification made under without departing from spirit and scope of the present utility model, all should be covered by the scope of the claims of the present utility model.
Claims (5)
1. a dressing with cell globoid, it is characterised in that include:
One template layer, it offers some aperture；And
One dressing layer, if crosslinking curing is embedded with stem cell globoid inside it in the aperture of this template layer.
There is the dressing of cell globoid the most as claimed in claim 1, it is characterised in that the material of described template layer is selected from silica gel or rubber.
There is the dressing of cell globoid the most as claimed in claim 1, it is characterised in that described dressing layer is gel.
There is the dressing of cell globoid the most as claimed in claim 1, it is characterised in that described cell globoid includes fat stem cell globoid.
There is the dressing of cell globoid the most as claimed in claim 4, it is characterised in that the number of described cell globoid is 5 × 105?.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW104219221U TWM522030U (en) | 2015-11-30 | 2015-11-30 | Dressing having regenerative stem cell spheroid |
| TW104219221 | 2015-11-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205795900U true CN205795900U (en) | 2016-12-14 |
Family
ID=56510613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521060431.3U Active CN205795900U (en) | 2015-11-30 | 2015-12-17 | Dressing with cell spheroids |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN205795900U (en) |
| TW (1) | TWM522030U (en) |
-
2015
- 2015-11-30 TW TW104219221U patent/TWM522030U/en unknown
- 2015-12-17 CN CN201521060431.3U patent/CN205795900U/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| TWM522030U (en) | 2016-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gentile et al. | Systematic review: adipose-derived mesenchymal stem cells, platelet-rich plasma and biomaterials as new regenerative strategies in chronic skin wounds and soft tissue defects | |
| Tavakoli et al. | Bioengineered skin substitutes: Advances and future trends | |
| Piaggesi et al. | Advanced therapies in wound management: cell and tissue based therapies, physical and bio-physical therapies smart and IT based technologies | |
| CN106109496B (en) | Human umbilical cord mesenchymal stem cells extract freeze-drying powder and preparation method | |
| Martínez-Santamaría et al. | Skin bioengineering: preclinical and clinical applications | |
| CN105013014A (en) | Preparation method and application of acellular matrix biological material | |
| CN105056303A (en) | Composition, preparation and application thereof | |
| CN103393585A (en) | Fibroblast liquid for beauty treatment and preparation method thereof | |
| CN102732586B (en) | Method for culturing mesenchymal stem cell secretin | |
| CN112263713A (en) | Fibrin hydrogel scaffold loaded with human umbilical cord mesenchymal stem cells and application thereof | |
| CN113564111B (en) | Method for culturing umbilical cord-derived mesenchymal stem cells in low-oxygen mode | |
| CN104087551A (en) | Novel method for in-vitro separated culture of human epidermal cells | |
| Huang et al. | Tissue-engineered skin: bottleneck or breakthrough | |
| CN107094752A (en) | A kind of tumor tissues storage in vitro liquid and preparation method thereof | |
| Parolini et al. | Human term placenta as a therapeutic agent: from the first clinical applications to future perspectives | |
| CN103421740B (en) | In-vitro culture and proliferation method for human mesenchymal stem cells | |
| CN103160434B (en) | An automatic cell culture device capable of simulating internal environments of organisms | |
| CN102172337B (en) | Tissue engineering skin with sebaceous gland-like structure and preparation method thereof | |
| Ye et al. | Fascia Layer—A Novel Target for the Application of Biomaterials in Skin Wound Healing | |
| CN106190946A (en) | A kind of culture medium for expansion of stem cells | |
| CN205795900U (en) | Dressing with cell spheroids | |
| CN104988111A (en) | Inducing liquid for converting UC-MSC into islet cells and application thereof | |
| CN201587946U (en) | In vitro co-culture apparatus for multiple types of cells | |
| RU2012135969A (en) | METHOD FOR TREATING A LONGLY HEALING Wound AND / OR WOUNDED CAVITY | |
| CN103736150A (en) | Application of melatonin synergistic extracellular matrix biomaterial to preparation of medicament for promoting osteoblast differentiation of mesenchymal stem cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210623 Address after: Hsinchu County, Taiwan, China Patentee after: Gwo Xi Stem Cell Applied Technology Co.,Ltd. Address before: Hsinchu County Patentee before: Ultra-Microrigin Biomedical Technology Co.,Ltd. |
|
| TR01 | Transfer of patent right |