CN205786025U - A kind of cell separation film-making dyeing all-in-one - Google Patents

A kind of cell separation film-making dyeing all-in-one Download PDF

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Publication number
CN205786025U
CN205786025U CN201620649317.2U CN201620649317U CN205786025U CN 205786025 U CN205786025 U CN 205786025U CN 201620649317 U CN201620649317 U CN 201620649317U CN 205786025 U CN205786025 U CN 205786025U
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China
Prior art keywords
suction pipe
dyeing
pin
workbench
separation film
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CN201620649317.2U
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Chinese (zh)
Inventor
刘召宏
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Nanjing Jingbang brocade medical science and Technology Co., Ltd.
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Nanjing Jianbang Jinyuan Medical Treatment Co Ltd
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Abstract

nullThe utility model discloses a kind of cell separation film-making dyeing all-in-one,Belong to cell chip preparing machine field,Aim to provide a kind of cell separation film-making dyeing all-in-one,There is the advantage making liquid basal cell will not mutually pollute in drawing transfer process,Its drip irrigation device one cell separation film-making dyeing all-in-one,Comprise workbench and be positioned at the Cartesian robot above workbench,Preparation bin is installed on workbench,Dyeing pin is installed on Cartesian robot,Described workbench is provided with liquid-holding pipe in the side of preparation bin,By support, vertical suction pipe is installed on workbench,The suction inlet of suction pipe down and is less than liquid-holding pipe bore,Described suction pipe and dyeing pin interference fit when the pin that dyes inserts suction pipe,Workbench is provided with upwardly extending curved baffle,The half slot horizontally entered into for dyeing pin is offered on curved baffle,The diameter of half slot is less than the bore of suction pipe upper end.

Description

A kind of cell separation film-making dyeing all-in-one
Technical field
This utility model relates to cell chip preparing machine field, particularly to a kind of cell separation film-making dyeing all-in-one.
Background technology
Cell separation film-making dyeing all-in-one by computer whole-process control, the gradient centrifugation in collection liquid-based cell sample manufacturing field, from So sedimentation, a dyeing technique are in one.
The Chinese patent of Publication No. CN205192825U discloses a kind of natural subsidence formula liquid base cell sheet producing machine, including Fuselage, described fuselage includes the workbench being positioned at bottom and is positioned at the Cartesian robot above workbench;Described workbench On slide apparatus for placing is installed, described slide apparatus for placing includes preparation bin, slide frame and slide, and described preparation bin is cylinder Shape, the lower end of preparation bin is provided with O, is provided with two ear handles, described ear handle and ear groove outside the lower end of preparation bin twist Composition screw thread pair;Described slide frame is provided with the slide groove mated with slide and with ear corresponding ear groove, described ear groove position Both sides in slide groove;Being provided with dyeing pin on described Cartesian robot, described dyeing pin includes liquid feeding pin and liquid pumping needle, Liquid feeding pin and liquid pumping needle is the most sheathed is fixed in an outer tube, the two ends of liquid feeding pin and the two ends of liquid pumping needle all protrude from outer tube, The lower end of described liquid pumping needle is provided with tilt port, and this utility model can improve slice-making quality and film-making efficiency.But in dyeing pin The liquid feeding pin in portion and liquid pumping needle are after extraction for the first time liquid basal cell, then are trapped in dye for the first time during extraction second time liquid basal cell Liquid basal cell within color pin can pollute second time liquid basal cell, causes cell separation impure.
Utility model content
The purpose of this utility model is to provide a kind of cell separation film-making dyeing all-in-one, has and makes liquid basal cell draw The advantage will not mutually polluted in transfer process.
Above-mentioned technical purpose of the present utility model has the technical scheme that a kind of cell separation film-making Dyeing all-in-one, comprises workbench and is positioned at the Cartesian robot above workbench, described workbench is provided with film-making Storehouse, described Cartesian robot is provided with dyeing pin, and described workbench is provided with liquid-holding pipe in the side of preparation bin, described Being provided with vertical suction pipe by support on workbench, the suction inlet of described suction pipe down and is less than liquid-holding pipe bore, works as dyeing Described suction pipe and dyeing pin interference fit when pin inserts suction pipe, described workbench is provided with upwardly extending curved baffle, described Offering the half slot horizontally entered into for dyeing pin on curved baffle, the diameter of described half slot is less than the bore of suction pipe upper end.
By using technique scheme, liquid-holding pipe is used for placing liquid basal cell, turns needing the cell by liquid-holding pipe During shifting, installing dyeing pin on Cartesian robot, Cartesian robot drives dyeing pin to move on the table, until Dyeing pin moves to the top of suction pipe, and suction pipe is vertically placed on the table by support, and therefore dyeing pin moves straight down Time dynamic, dyeing pin may be inserted into inside suction pipe, and suction pipe and dyeing pin interference fit when the pin that dyes inserts suction pipe, therefore dye pin Can combine closely with suction pipe, when the pin that dyes moves up, drive suction pipe be also moved up and separated with support, right angle is sat Scalar robot drive dyeing pin move and then drive suction pipe to move to liquid-holding pipe upper end, now be moved down by dyeing pin, now by Suction inlet in suction pipe is less than liquid-holding pipe bore, and therefore suction pipe will enter the liquid-holding pipe liquid basal cell sucking-off by its inside, then Cartesian robot drives dyeing pin to move to preparation bin direction, until moving to preparation bin upper end, now rectangular coordinate machine Device people drives dyeing pin to move down, and then drives suction pipe to be moved down into inside preparation bin, is put by the liquid basal cell in suction pipe Carrying out in entering preparation bin precipitating film-making, after liquid basal cell is put into preparation bin by suction pipe, Cartesian robot drives dyeing Pin moves up, and then moves to the direction of curved baffle, until dyeing pin being moved in the half slot of curved baffle, and dyeing Pin moves up, and owing to the diameter of half slot is less than the bore of suction pipe upper end, therefore half slot will stop the suction pipe on dyeing pin Moving with tinction pin, and then separated with dyeing pin by suction pipe, dyeing pin, by suction pipe liquid draw basal cell, is drawing another Different suction pipes is changed, it is ensured that the liquid basal cell entered in preparation bin is not contaminated, Jin Erbao before liquid basal cell in liquid-holding pipe The purity of card liquid basal cell.
This utility model is further arranged to: described dyeing pin outer wall is provided with rubber blanket in the position entering half slot.
By using technique scheme, the setting of rubber blanket slow down dyeing pin and bumps against half slot when entering half slot Cell wall on power, it is to avoid dyeing pin hit curved by the cell wall of half slot.
This utility model is further arranged to: be provided with electric shock switch module, described electric shock switch module in described rubber blanket Including positive plate and negative plate, described positive plate and negative plate along half slot be radially arranged and between be provided with gap, described positive pole Plate and negative plate are all connected with alarm by wire.
By using technique scheme, positive plate in electric shock switch module and negative plate owing to radially setting along half slot Put and between be provided with gap, when therefore the gap between positive plate and negative plate bumps against half slot with tinction pin rubber blanket depression And reduce, now positive plate makes the circuit that positive plate and negative plate are connected with alarm by wire with negative plate by contacting Be in closure state, and then make alarm equipment alarm, notify operator in time stop dye pin movement, protection dyeing pin not by Damage.
This utility model is further arranged to: be provided with horizontal water fender in described suction pipe, and described water fender periphery is provided with Some connecting rods, one end of described connecting rod is connected with water fender, and the other end is connected with suction pipe inwall.
By using technique scheme, when the pin that dyes moves to liquid-holding pipe together with suction pipe, dyeing pin is by liquid-holding pipe Interior cell is drawn onto in suction pipe, and the now setting of the horizontal water fender in suction pipe reduces the machine that Cell sap is sucked in dyeing pin Meeting, it is to avoid dyeing pin is contaminated, and the setting of connecting rod makes water fender be fixed in suction pipe, strengthens the company of water fender and suction pipe Connect.
This utility model is further arranged to: described workbench is provided with the suction dropped for placement in the lower end of curved baffle The container of pipe.
By using technique scheme, the setting of container is put when being easy under the suction pipe gear that curved baffle will dye on pin Put suction pipe, it is to avoid the Cell sap in suction pipe flows to collect on workbench suction pipe simultaneously.
This utility model is further arranged to: described container cassette bottom is provided with absorbent cotton.
By using technique scheme, the Cell sap of residual in suction pipe when flowing to the cassette bottom of container is inhaled by absorbent cotton Receive, it is to avoid the Cell sap of residual is splash by suction pipe when dropping the cassette bottom of container, causes the pollution of workbench.
This utility model is further arranged to: the lower end of described suction pipe is set to cone.
By using technique scheme, the lower end of suction pipe is set to cone and is easy to the absorption of Cell sap, simultaneously facilitate In suction pipe enters liquid-holding pipe and inside preparation bin.
This utility model is further arranged to: described dyeing pin is provided with wearing layer in the one end contacted with suction pipe.
By using technique scheme, dyeing pin is often in close contact with suction pipe, and therefore dyeing pin is easy and between suction pipe Produce friction, be provided with wearing layer at dyeing pin in the one end contacted with suction pipe, it is to avoid dyeing pin is worn, it is ensured that dyeing pin and suction Pipe closely cooperates.
This utility model is further arranged to: described curved baffle is provided with wear-resistant pad in the lower end of the position of half slot.
By using technique scheme, under the suction pipe on dyeing pin is kept off by curved baffle, the therefore lower end of curved baffle Easily produce rub with suction pipe upper end, and then curved baffle lower end is worn, reduce the intensity of curved baffle, will be unfavorable for Under the gear of suction pipe, curved baffle is provided with wear-resistant pad in the lower end of the position of half slot, and curved baffle quilt is avoided in the setting of wear-resistant pad Abrasion, it is ensured that the normal use of curved baffle.
This utility model is further arranged to: described curved baffle lower end is provided with bracing piece, one end of described bracing piece with Curved baffle connects, and the other end is connected with workbench.
By using technique scheme, when dyeing pin enters the half slot of curved baffle, move up, and then utilize half Circular groove produces downward thrust to suction pipe, and now suction pipe also produces thrust upwards to curved baffle so that curved baffle is oriented The trend of upper movement, easily makes curved baffle stretch, is unfavorable under the gear of suction pipe, therefore in curved baffle lower end after life-time service Being provided with bracing piece, the setting of bracing piece reduces the deformation of curved baffle.
In sum, this utility model has the advantages that the setting of curved baffle and half slot is easy to contaminate Under suction pipe gear on color pin, on dyeing pin, the protection dyeing pin that arranges of rubber blanket is not broken by half slot, the positive pole in rubber blanket Plate contacts so that the siren being connected with positive plate and negative plate sounds with negative plate when the pin that dyes bumps against the cell wall of half slot, Operator is reminded to stop machine in time.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment 1;
Fig. 2 be embodiment 1 for embody dyeing pin and suction pipe coordinate structural representation;
Fig. 3 be embodiment 1 for embodying the structural representation of water fender in suction pipe;
Fig. 4 is the structural representation of embodiment 2;
Fig. 5 be embodiment 2 for embodying the structural representation within rubber blanket;
Fig. 6 is the structural representation of embodiment 3.
In figure, 1, workbench;11, preparation bin;12, liquid-holding pipe;13, suction pipe;131, cone;132, water fender;133、 Connecting rod;14, curved baffle;141, half slot;142, wear-resistant pad;2, Cartesian robot;21, dyeing pin;211, wearing layer; 212, rubber blanket;2121, positive plate;2122, negative plate;214, alarm;15, bracing piece;16, container;161, absorbent cotton.
Detailed description of the invention
Below in conjunction with accompanying drawing, this utility model is described in further detail.
Embodiment 1: a kind of cell separation film-making dyeing all-in-one, sees figures.1.and.2, is provided above right angle at workbench 1 Coordinates robot 2, are provided with dyeing pin 21 on Cartesian robot 2, are provided with preparation bin 11 and peace on workbench 1 Being contained in the liquid-holding pipe 12 of preparation bin 11 side, workbench 1 is provided with vertical suction pipe 13 in the side of liquid-holding pipe 12 by support, The suction inlet of suction pipe 13 is arranged and down less than liquid-holding pipe 12 and the bore of preparation bin 11, and the lower end of suction pipe 13 is set to circle Cone 131, it is simple to the absorption of Cell sap, simultaneously facilitates suction pipe 13 and rapidly enters liquid-holding pipe 12 and preparation bin 11 inside, work as dyeing Suction pipe 13 and dyeing pin 21 interference fit when pin 21 inserts suction pipe 13, it is simple to suction pipe 13 is fixed on dyeing pin 21, with tinction The movement of pin 21 and move;It is provided with wearing layer 211 in the one end contacted with suction pipe 13, it is to avoid dyeing pin 21 is ground at dyeing pin 21 Damage, it is ensured that dyeing pin 21 closely cooperates with suction pipe 13;In order to suction pipe 13 is taken off, therefore on workbench 1 from dyeing pin 21 Upwardly extending curved baffle 14 is set, and on curved baffle 14, offers the half slot 141 horizontally entered into for dyeing pin 21, half The diameter of circular groove 141 is less than the bore of suction pipe 13 upper end, under being beneficial to be kept off from dyeing pin 21 by suction pipe 13 by curved baffle 14; Curved baffle 14 is provided with wear-resistant pad 142 in the lower end of the position of half slot 141, it is to avoid curved baffle 14 is worn and reduces arc The intensity of shape baffle plate 14, it is ensured that the normal use of curved baffle 14;In the lower end of curved baffle 14, bracing piece 15, bracing piece are set One end of 15 is connected with curved baffle 14, and the other end is connected with workbench 1.
With reference to Fig. 3, in this external suction pipe 13, it is provided with horizontal water fender 132, when dyeing pin 21 is inserted in suction pipe 13 Time, water fender 132 is positioned at dyeing pin 21 lower end, and water fender 132 periphery is provided with some connecting rods 133, connecting rod 133 one end and water fender 132 fixing connections, the other end is fixing with suction pipe 13 inwall to be connected, and the cell in liquid-holding pipe 12 is drawn onto suction pipe 13 by dyeing pin 21 In, the now setting of the horizontal water fender 132 in suction pipe 13 reduces the chance that Cell sap is sucked in dyeing pin 21, it is to avoid dye Color pin 21 is contaminated, and arranging of connecting rod 133 ensures suction pipe 13 normal absorption Cell sap, strengthens water fender 132 and suction pipe simultaneously The connection of 13.
Specific operation process: when needing the cellular liquid in liquid-holding pipe 12 is transferred to preparation bin 11, rectangular coordinate machine Device people 2 drives dyeing pin 21 to shift to the top of suction pipe 13, and now dyeing pin 21 moves straight down, and dyeing pin 21 inserts suction pipe 13 Internal and with suction pipe 13 compact siro spinning technology, avoid dyeing pin at the dyeing pin 21 wearing layer 211 in the position contacted with suction pipe 13 The abrasion of 21, it is ensured that the compact siro spinning technology of dyeing pin 21 and suction pipe 13, when the pin 21 that dyes moves up, will drive suction pipe 13 also to Upper movement also separates with support, and Cartesian robot 2 drives dyeing pin 21 move and then drive suction pipe 13 to move to liquid-holding pipe 12 Upper end, now moves down dyeing pin 21 so that suction pipe 13 enters in liquid-holding pipe 12, and suction pipe 13 is by the liquid within liquid-holding pipe 12 Basal cell's sucking-off, now the water fender 132 in suction pipe 13 stops cell to enter in dyeing pin 21, then Cartesian robot 2 Dyeing pin 21 is driven to move up so that suction pipe 13 removes in liquid-holding pipe 12;Drive dyeing pin 21 to preparation bin 11 direction again Mobile, until moving to preparation bin 11 upper end, now Cartesian robot 2 drives dyeing pin 21 to move down, and then drives It is internal that suction pipe 13 is moved down into preparation bin 11, the liquid basal cell in suction pipe 13 is put into preparation bin 11 and carries out precipitating film-making, After liquid basal cell is put into preparation bin 11 by suction pipe 13, Cartesian robot 2 drives dyeing pin 21 to move up so that inhale Pipe 13 removes in preparation bin 11;Dyeing pin 21 is driven to move to the direction of curved baffle 14, until being moved by dyeing pin 21 again In the half slot 141 of curved baffle 14, now suction pipe 13 is in the lower section of half slot 141, when dyeing pin 21 moves up partly The suction pipe 13 stopped on dyeing pin 21 is moved by circular groove 141 with tinction pin 21, and then is separated with dyeing pin 21 by suction pipe 13, enters Row circulates next time, and dyeing pin 21 is by suction pipe 13 liquid draw basal cell, the liquid basal cell in drawing different liquid-holding pipes 12 Suction pipe 13 different for Shi Genghuan, it is ensured that the liquid basal cell entered in preparation bin 11 is not contaminated, and then ensure the pure of liquid basal cell Degree.
When this external dyeing pin 21 moves up in moving to half slot 141, suction pipe 13 is produced downward by half slot 141 Thrust, now suction pipe 13 also produces thrust upwards to curved baffle 14 so that curved baffle 14 has the trend moved up, and adds Strong bar 15 makes curved baffle 14, when having the trend moved up, curved baffle 14 be produced downward pulling force, and then reduces arc The deformation of shape baffle plate 14, extends the service life of curved baffle 14.
Embodiment 2: a kind of cell separation film-making dyeing all-in-one, is with the difference of embodiment 1, with reference to Fig. 4 and Fig. 5, dyeing pin 21 outer wall is provided with rubber blanket 212 in the position entering half slot 141, slows down dyeing pin 21 and half slot 141 Collision, protection dyeing pin 21;Electric shock switch module it is provided with, including positive plate 2121 and negative plate 2122, just in rubber blanket 212 Pole plate 2121 and negative plate 2122 are all set to annular, increase the contact area of positive plate 2121 and negative plate 2122, are just being beneficial to Pole plate 2121 contacts with negative plate 2122, by positive plate 2121 and negative plate 2122 along half slot 141 be radially arranged and between It is provided with gap, is easy to positive plate 2121 when rubber blanket 212 deforms and contacts with negative plate 2122, positive plate 2121 and negative plate 2122 are all connected with alarm 214 by wire.
Specific operation process: rubber blanket 212 caves in and reduces positive plate 2121 when the pin 21 that dyes bumps against half slot 141 And the gap between negative plate 2122 so that positive plate 2121 will contact with negative plate 2122 and make positive plate 2121 and negative pole Plate 2122 is in closure state by the circuit that wire is connected with alarm 214, and then alarm 214 is reported to the police, notice behaviour Author stops the movement of dyeing pin 21 in time, and protection dyeing pin 21 is not damaged, and is moved away from by dyeing pin 21 operator During the position of half slot 141, the rubber blanket 212 on dyeing pin 21 resiles, now positive plate 2121 and negative plate 2122 it Between gap be gradually increased, and then make the circuit that positive plate 2121 and negative plate 2122 be connected with alarm 214 by wire Being off, now alarm 214 is not reported to the police, and machine is in normal operating conditions.
Embodiment 3: a kind of cell separation film-making dyeing all-in-one, is with the difference of embodiment 2, with reference to Fig. 6, work Station 1 is provided with the container 16 for placing the suction pipe 13 dropped in the lower end of curved baffle 14, it is to avoid the cell in suction pipe 13 Liquid stream collects suction pipe 13 on workbench 1 simultaneously, and the cassette bottom of this external container 16 is provided with absorbent cotton 161, and absorbent cotton 161 will stream In the suction pipe 13 during the cassette bottom of container 16, the Cell sap of residual absorbs, it is to avoid suction pipe 13 is when dropping the cassette bottom of container 16 The Cell sap of residual is splash, causes the pollution of workbench 1.
This specific embodiment is only that it is not to restriction of the present utility model, ability to explanation of the present utility model The present embodiment can be made after reading this specification by field technique personnel as required does not has the amendment of creative contribution, but As long as all being protected by Patent Law in right of the present utility model.

Claims (10)

1. a cell separation film-making dyeing all-in-one, comprises workbench (1) and is positioned at the rectangular coordinate machine of workbench (1) top Device people (2), described workbench (1) is provided with preparation bin (11), and described Cartesian robot is provided with dyeing pin on (2) (21), it is characterised in that: described workbench (1) is provided with liquid-holding pipe (12), described workbench (1) in the side of preparation bin (11) On by support, vertical suction pipe (13) is installed, the suction inlet of described suction pipe (13) down and less than liquid-holding pipe (12) bore, Described suction pipe (13) and dyeing pin (21) interference fit when the pin (21) that dyes inserts suction pipe (13), described workbench sets on (1) There is upwardly extending curved baffle (14), described curved baffle (14) offers the half slot horizontally entered into for dyeing pin (21) (141), the diameter of described half slot (141) is less than the bore of suction pipe (13) upper end.
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: described dyeing pin (21) Outer wall is provided with rubber blanket (212) in the position entering half slot (141).
A kind of cell separation film-making dyeing all-in-one the most according to claim 2, it is characterised in that: described rubber blanket (212) being provided with electric shock switch module in, described electric shock switch module includes positive plate (2121) and negative plate (2122), described just Pole plate (2121) and negative plate (2122) along half slot (141) be radially arranged and between be provided with gap, described positive plate (2121) All it is connected with alarm (214) by wire with negative plate (2122).
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: in described suction pipe (13) Being provided with horizontal water fender (132), described water fender (132) periphery is provided with some connecting rods (133), the one of described connecting rod (133) End is connected with water fender (132), and the other end is connected with suction pipe (13) inwall.
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: described workbench (1) The container (16) for placing the suction pipe (13) dropped it is provided with in the lower end of curved baffle (14).
A kind of cell separation film-making dyeing all-in-one the most according to claim 5, it is characterised in that: described container (16) Cassette bottom is provided with absorbent cotton (161).
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: described suction pipe (13) Lower end is set to cone (131).
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: described dyeing pin (21) It is provided with wearing layer (211) in the one end contacted with suction pipe (13).
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: described curved baffle (14) lower end in the position of half slot (141) is provided with wear-resistant pad (142).
A kind of cell separation film-making dyeing all-in-one the most according to claim 1, it is characterised in that: described curved baffle (14) lower end is provided with bracing piece (15), and one end of described bracing piece (15) is connected with curved baffle (14), the other end and workbench (1) connect.
CN201620649317.2U 2016-06-25 2016-06-25 A kind of cell separation film-making dyeing all-in-one Active CN205786025U (en)

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CN201620649317.2U CN205786025U (en) 2016-06-25 2016-06-25 A kind of cell separation film-making dyeing all-in-one

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Application Number Priority Date Filing Date Title
CN201620649317.2U CN205786025U (en) 2016-06-25 2016-06-25 A kind of cell separation film-making dyeing all-in-one

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112461631A (en) * 2020-10-27 2021-03-09 安徽九陆生物科技有限公司 Dyeing needle lifting device for liquid-based thin-layer cell sheet-making dyeing machine
CN113267396A (en) * 2021-07-16 2021-08-17 易普森生物科技(深圳)有限公司 Cell film-making dyeing machine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112461631A (en) * 2020-10-27 2021-03-09 安徽九陆生物科技有限公司 Dyeing needle lifting device for liquid-based thin-layer cell sheet-making dyeing machine
CN113267396A (en) * 2021-07-16 2021-08-17 易普森生物科技(深圳)有限公司 Cell film-making dyeing machine

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C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20171208

Address after: 211800 Nanjing, Nanjing, Jiangsu Province, Pukou District, Nanjing Zijin (Pukou) technology entrepreneurship special community Tai Zhong Road

Patentee after: Nanjing Jingbang brocade medical science and Technology Co., Ltd.

Address before: 211800 Jiangsu, Nanjing Zijin (Pukou) science and technology venture special community road, No. 99, room 6, building No. 218

Patentee before: Nanjing Jianbang Jinyuan Medical Treatment Co., Ltd.

TR01 Transfer of patent right