CN205785912U - A kind of water body environment DNA sampling apparatus - Google Patents
A kind of water body environment DNA sampling apparatus Download PDFInfo
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- CN205785912U CN205785912U CN201620486713.8U CN201620486713U CN205785912U CN 205785912 U CN205785912 U CN 205785912U CN 201620486713 U CN201620486713 U CN 201620486713U CN 205785912 U CN205785912 U CN 205785912U
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- protective cover
- filter paper
- pumping
- pumping conduit
- flabellum
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Abstract
The utility model discloses a kind of water body environment DNA sampling apparatus, including water pumper and the pumping conduit that is attached thereto, the other end of pumping conduit is provided with filter paper, filter paper is fixed by retainer ring, this device also includes protective cover and pumping rod, protective cover is arranged on pumping conduit, and pumping rod connects protective cover by strut.Further, described retainer ring includes ring for fixing, and it is more than the external diameter of pumping conduit；Retainer ring also includes multiple flabellum for covering filter paper, and multiple flabellums cooperatively form circle, and one of them angle of flabellum is connected with ring for fixing axle, and the edge of flabellum is connected to protective cover also by connecting tube；Described protective cover includes necklace and the movable branching rod of bottom, and necklace is arranged on pumping conduit, and movable branching rod is connected with strut.This utility model can complete the collection in worksite work of water body environment DNA, it is not necessary to follow-up heavy filter operation, time saving and energy saving.
Description
Technical field
This utility model relates to environment DNA detection technique field, refers specifically to a kind of water body environment DNA sampling apparatus.
Background technology
The application of environment DNA is extracting directly DNA genome from soil, deposit the earliest, it is used for studying the most educable microbial diversity and species identification, in recent years, gradually expand to zoology and botany research field from microbiology, in amphibian, Fish, insect larvae, shell-fish and mammal are studied, all have well application.In water body environment, utilizing environment DNA technology can well judge the distribution situation of species, analyze the structure of community of species, particularly in the case of target species biological density is relatively low, environment DNA technology can well capture target species DNA, in order to analyze and research.Liu Qigen etc. utilize eDNA method according to waters, Fish Communities place size, gather water sample, use 5 for 16s rDNA, COI, Cytb1, Cytb4, D-loop district, primer is expanded Fish sample, obtain the Fish Community Structures of reservoir area, Thousand-Island Lake, be consistent with monitoring and surveying result over the years.The secondary sequencing technologies used, it is possible to obtain ten thousand 16s rDNA band numbers of 1-2, such a large-scale sequencing analysis technology in a PCR sample, efficiency far is higher than the sample number that we are obtained when conventional catching method.Compared with traditional investigation method, environment DNA technology greatly reducing statistical work, adds the accuracy of research, reduces the cost of sample collecting under fixing sample number simultaneously.
Advantage in view of above-mentioned environment DNA technology, increasing research starts with environment DNA and carries out correlation study research, at present, general employing first collects water body sample, and the mode then filtering water sample collects water body DNA, from the point of view of natural water, the method needs longer filtration time, and during operation in the wild, if there is no condition of power supply on operation ship, water body sample must be taken back land to filter, the amount of labour is bigger.So, one can be with on-site collection DNA, and the harvester facilitating research worker to carry water body DNA sample is the most necessary.
Summary of the invention
The purpose of this utility model is for problem above, it is provided that a kind of water body environment DNA sampling apparatus；Facilitate under water body environment, the collecting work of DNA.
For solving above-mentioned technical problem, this utility model be the technical scheme is that a kind of water body environment DNA sampling apparatus, including water pumper and the pumping conduit that is attached thereto, the other end of pumping conduit is provided with filter paper, filter paper is fixed by retainer ring, this device also includes protective cover and pumping rod, and protective cover is arranged on pumping conduit, and pumping rod connects protective cover by strut.
Further, described retainer ring includes ring for fixing, and it is more than the external diameter of pumping conduit；Retainer ring also includes multiple flabellum for covering filter paper, and multiple flabellums cooperatively form circle, and one of them angle of flabellum is connected with ring for fixing axle, and the edge of flabellum is connected to protective cover also by connecting tube.
Further, described protective cover includes necklace and the movable branching rod of bottom, and necklace is arranged on pumping conduit, and movable branching rod is connected with strut.
Further, described movable branching rod is many, and its one end is uniformly fixed on necklace.
Described water pumper is also associated with volumetric flask.
Described pumping conduit is provided with the mouth of pipe of one end of filter paper upward.
Described filter paper is nitrocellulose quantitative filter paper, pore size can be formulated according to experiment demand, filter paper retainer ring is used for fastening filter paper, preventing and treating filter paper landing because of water impact, protective cover is used for intercepting some large-scale pollutant, such as blade, the house refuses such as plastic bag, preventing filter paper mouth blocked, pumping rod, in order to seal filter paper retainer ring, reduces the DNA loss amount that during pulling up, filter paper is collected.This utility model is operated by pumping rod, when instantly pushing away pumping rod, can make flabellum separately, make filter paper expose, now open water pumper and fetch water；On the contrary, when on carry pumping rod time, strut regain, drive coupled movable branching rod to tighten up, then make the connecting tube being connected with protective cover inwardly promote flabellum, finally make filter paper be enclosed in retainer ring.
The beneficial effect that this utility model is reached:
1) the collection in worksite work of water body environment DNA can be completed, it is not necessary to follow-up heavy filter operation, time saving and energy saving.
2) this device protective effect to filter paper, effectively reduces the loss of the DNA being collected on filter paper.
3) this device can quantitative collection water body, facilitate scientific research personnel to carry out relevant quantitative experiment.
4) which solves collection in worksite, the problem of collection water body DNA sample, facilitate follow-up study, be worthy to be popularized.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the schematic diagram after this utility model filter paper retainer ring is drawn in.
Fig. 3 is the schematic diagram after this utility model filter paper retainer ring is launched.
Detailed description of the invention
Further illustrate this utility model below in conjunction with embodiment, but the scope that this utility model claims is not limited to the scope that embodiment is stated.
As shown in Figure 1, a kind of water body environment DNA sampling apparatus, including water pumper 1 and the pumping conduit 2 that is attached thereto, the other end of pumping conduit is provided with filter paper, filter paper is fixed by retainer ring 3, this device also includes protective cover 4 and pumping rod 5, and protective cover is arranged on pumping conduit, and pumping rod 5 connects protective cover by strut 51.
Described retainer ring 3 includes ring for fixing 31, and it is more than the external diameter of pumping conduit；Retainer ring 3 also includes multiple flabellum 32 for covering filter paper, and multiple flabellums cooperatively form circle, and one of them angle of flabellum is connected with ring for fixing axle, and the edge of flabellum is connected to protective cover 4 also by connecting tube 33.
Described protective cover 4 includes necklace 41 and the movable branching rod 42 of bottom, and necklace 41 is arranged on pumping conduit 2, and movable branching rod 42 is connected with strut 51.
Described movable branching rod 42 is many, and its one end is uniformly fixed on necklace 41.
Described water pumper 1 is also associated with volumetric flask 6.
Described pumping conduit is provided with the mouth of pipe of one end of filter paper upward.
During operation, pumping conduit one end is connected with water pumper, the ring for fixing in retainer ring is utilized to be fixed on by filter paper on the pumping conduit end mouth of pipe under water, being covered on by protective cover in retainer ring, protective cover is fixed on pumping conduit by necklace, under push away pumping rod, strut is made to be in open configuration, then making to draw connecting tube outside the movable branching rod of protective cover, make flabellum 8 open, filter paper exposes.Said apparatus is put under water, open water pumper, after water body is via filter paper filtering, from the outlet of water pumper, flow in volumetric flask, after the water body collected in volumetric flask reaches the sample volume amount of required collection, close water pumper, pull up pumping rod, strut reclaims, drive coupled movable branching rod to tighten up, then make connecting tube inwardly promote flabellum, finally make filter paper be enclosed in retainer ring.Being left the water by device, taking-up filter paper is put in dehydrated alcohol and is preserved, in order to extract DNA.
Claims (6)
1. a water body environment DNA sampling apparatus, it is characterized in that: include water pumper (1) and the pumping conduit (2) being attached thereto, the other end of pumping conduit is provided with filter paper, filter paper is fixed by retainer ring (3), this device also includes protective cover (4) and pumping rod (5), protective cover is arranged on pumping conduit, and pumping rod (5) connects protective cover by strut (51).
Device the most according to claim 1, it is characterised in that: described retainer ring (3) includes ring for fixing (31), and it is more than the external diameter of pumping conduit；Retainer ring (3) also includes multiple flabellum (32) for covering filter paper, and multiple flabellums cooperatively form circle, and one of them angle of flabellum is connected with ring for fixing axle, and the edge of flabellum is connected to protective cover (4) also by connecting tube (33).
Device the most according to claim 2, it is characterised in that: described protective cover (4) includes necklace (41) and the movable branching rod (42) of bottom, and necklace (41) is arranged on pumping conduit (2), and movable branching rod (42) is connected with strut (51).
Device the most according to claim 3, it is characterised in that: described movable branching rod (42) is many, and its one end is uniformly fixed on necklace (41).
Device the most according to claim 1, it is characterised in that: described water pumper (1) is also associated with volumetric flask (6).
Device the most according to claim 1, it is characterised in that: described pumping conduit is provided with the mouth of pipe of one end of filter paper upward.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620486713.8U CN205785912U (en) | 2016-05-25 | 2016-05-25 | A kind of water body environment DNA sampling apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620486713.8U CN205785912U (en) | 2016-05-25 | 2016-05-25 | A kind of water body environment DNA sampling apparatus |
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| CN205785912U true CN205785912U (en) | 2016-12-07 |
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| CN201620486713.8U Active CN205785912U (en) | 2016-05-25 | 2016-05-25 | A kind of water body environment DNA sampling apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109406215A (en) * | 2018-12-28 | 2019-03-01 | 南京大学 | A kind of water body environment DNA intelligent acquisition device and acquisition method |
| CN111607505A (en) * | 2020-06-04 | 2020-09-01 | 华南农业大学 | Water sample collecting and filtering device applied to estuary environment DNA research |
-
2016
- 2016-05-25 CN CN201620486713.8U patent/CN205785912U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109406215A (en) * | 2018-12-28 | 2019-03-01 | 南京大学 | A kind of water body environment DNA intelligent acquisition device and acquisition method |
| CN109406215B (en) * | 2018-12-28 | 2024-02-09 | 南京大学 | Intelligent acquisition device and acquisition method for water body environment DNA |
| CN111607505A (en) * | 2020-06-04 | 2020-09-01 | 华南农业大学 | Water sample collecting and filtering device applied to estuary environment DNA research |
| CN111607505B (en) * | 2020-06-04 | 2021-09-21 | 华南农业大学 | Water sample collecting and filtering device applied to estuary environment DNA research |
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