CN205749537U - A kind of micro-fluidic chemiluminescence immunoassay detection device - Google Patents
A kind of micro-fluidic chemiluminescence immunoassay detection device Download PDFInfo
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- CN205749537U CN205749537U CN201620440535.5U CN201620440535U CN205749537U CN 205749537 U CN205749537 U CN 205749537U CN 201620440535 U CN201620440535 U CN 201620440535U CN 205749537 U CN205749537 U CN 205749537U
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Abstract
The utility model discloses a kind of micro-fluidic chemiluminescence immunoassay detection device, including micro-fluidic disc, being provided with miniflow detector unit on described micro-fluidic disc, described miniflow detector unit includes that whole blood injects groove, whole blood separation channel, blood cell accumulator tank, blood plasma transmission runner, mixing/detecting groove, the first reagent accumulator tank, cleanout fluid injection groove, gas compression release groove, waste liquid tank, the second reagent accumulator tank, the 4th reagent accumulator tank.The wisdom that this utility model uses simple method can realize the internal microfluid of micro-fluidic disc controls, and reaction liquid can be made to be sufficiently mixed, it is ensured that reaction system is efficiently carried out, and quickly realizes the detection by quantitative of hCG concentration in sample simultaneously；Having simple to operate, detection sensitivity is high, and result is accurately and reliably, reproducible, the feature of low cost.
Description
Technical field
This utility model relates to a kind of micro-fluidic chemiluminescence immunoassay detection device, and particularly one is entered under centrifugation systems
Row whole blood separates, blood plasma quantitatively, mixing (cultivation), the immunoreation step of cleaning.
Background technology
Chemiluminescence immune assay, is also called cold light, refer in the case of there is no any light, heat or electric field equal excitation by
Chemical reaction and the light radiation that produces, be by the antigen-antibody immunity of highly sensitive chemiluminescence detection technology with high specific
Reaction bonded is got up, and uses antigen or the content of antibody in detection measured object.Owing to need not external excitation light source, the back of the body can be avoided
Scape disturbs and signal to noise ratio is greatly improved.Can be used for the detections such as various antigen, antibody, hormone, enzyme, fatty acid, vitamin and medicine,
Can be the important developing direction of immunoassay as the substituent of radioimmunoassay, RIA Yu enzyme immunoassay.Chemiluminescence is exempted from
Epidemic disease analysis comprises two key components, respectively immune response system and chemiluminescence analysis system.Immune response system
It is the ultimate principle according to antigen antibody reaction, luminescent substance is marked directly on antigen or antibody, or be used for enzyme sending out
Light substrate；Chemiluminescence analysis system is to utilize chemiluminescent substance to be formed excite through the catalysis of catalyst and the oxidation of oxidant
State, when the excited state molecule of this instability returns to stable ground state, releases energy and launches photon, utilize photon signal
Detector measures the luminous intensity of luminescence-producing reaction, thus calculates measured matter content.
Chemiluminescence immune assay step is numerous and diverse and time-consuming: (one), need to add ferment in capture antibodies, antigen, link successively
The detecting antibody of element, cleanout fluid, substrate.(2), need between each step to hatch and cleaning step.(3), clinical sample (whole blood)
Sample pretreatment need to be carried out in advance, after high speed centrifugation, take out serum, can test.Therefore, be integrated into simple and
Quickly detection method is an important problem.
Utility model content
This utility model purpose there are provided a kind of micro-fluidic chemiluminescence immunoassay detection device；This utility model is a kind of
Micro-fluidic chemiluminescence immunoassay detection device has efficient, stable, easy feature, (can be not required to whole blood sample direct injected
Extra Sample pretreatment), whole blood separation, quantitative blood plasma transmission, mixing (cultivation) can be reached by this utility model, clean
Immunoreation step.
In order to achieve the above object, the technical solution of the utility model is:
A kind of micro-fluidic chemiluminescence immunoassay detection device, including micro-fluidic disc, described micro-fluidic disc is installed
Whole blood injects groove to have miniflow detector unit, described miniflow detector unit to include, described whole blood is injected groove and separated by whole blood
Channel is connected with blood cell accumulator tank, and described blood cell accumulator tank transmits flow passage with blood plasma；Described blood plasma transmits runner with mixed
Close/detect groove top to be connected；Described mixing/detecting groove top is connected by pipeline and the first reagent accumulator tank；Described is mixed
Close/detect groove top to connect also by pipeline and the 3rd reagent accumulator tank；Described mixing/detecting groove side is provided with gas pressure
Contracting release groove；Described mixing/detecting trench bottom is connected by pipeline and the first waste liquid tank, and described mixing/detecting bottom land
First valve is installed on the pipeline between portion and the first waste liquid tank；The first described reagent accumulator tank top is by pipeline and the
The bottom connection of two reagent accumulator tanks；The 3rd described reagent accumulator tank is connected by the bottom of pipeline and the 4th reagent accumulator tank
Logical.
Described whole blood injects groove and connects with blood cell accumulator tank；Described blood plasma transmits runner and mixes/detect groove top
Between be also equipped with blood plasma quantitative slot；The first through runner and adjacent miniflow detector unit are passed through in described blood plasma quantitative slot top
On blood plasma quantitative slot top connection；Lead to the most successively between bottom and the 3rd reagent accumulator tank of the 4th described reagent accumulator tank
Cross the second through runner, the 4th reagent quantitative groove, the 3rd through runner are connected, and the 4th described reagent quantitative groove top passes through
Second through runner and the 4th reagent quantitative groove top connection in adjacent miniflow detector unit；The 3rd described reagent accumulator tank
Bottom is connected by pipeline with mixing/detecting groove top after connecting with blood plasma quantitative slot again, and the 3rd described reagent accumulator tank
Bottom with also pass sequentially through the 3rd through runner between blood plasma quantitative slot, the 3rd reagent quantitative groove, the first through runner are connected；And
The 3rd through runner and the 3rd reagent quantitative groove in adjacent miniflow detector unit are passed through in the 3rd described reagent quantitative groove top
Top connects；Described the first through runner, the second through runner, the 3rd through runner connect with the second waste liquid tank respectively.
Described blood plasma quantitative slot is provided with the second valve with on the pipeline mixing/detecting between groove；The 3rd described examination
On pipeline between agent quantitative slot and the first through runner, the 3rd valve is installed；Described the 4th reagent quantitative groove and the 3rd passes through
4th valve is installed on the pipeline between passage flow duct.
The miniflow detector unit of more than 4 is installed on described micro-fluidic disc.
4 or 12 miniflow detector units are preferably installed on described micro-fluidic disc.
The beneficial effects of the utility model are: this utility model a kind of micro-fluidic chemiluminescence immunoassay detection device has height
Effect, stable, easy feature, can be with whole blood sample direct injected (being not required to extra Sample pretreatment), can be new by this practicality
Type reaches whole blood separation, quantitative blood plasma transmission, mixing (cultivation), the immunoreation step of cleaning.Whole blood separates and blood plasma is quantitative
Design: by centrifugal rotational speed control, it can be ensured that the separation efficiency of high Hematocrit sample (blood cell: 80 %, blood plasma: 20 %) (can
It is kept completely separate blood plasma and blood cell), also can ensure that the quantitative effect (coefficient of variation CV < 3 %) of blood plasma.Immunity microparticle operation sets
Meter: by gas (compression/release) groove and centrifugal rotational speed manipulation, gas compression and release can be controlled, thereby produce disturbance
(immunity microparticle), to reach good mixing (cultivation) effect.Immunity microparticle retains design: combine gate design, can
Immunity microparticle is remained in mixing/detecting groove.The material of immunity microparticle can be metal micro particles, plastics microparticle
Deng.Microparticle size is more than 50 microns.
Accompanying drawing explanation
Fig. 1 is the micro-fluidic disc of a kind of micro-fluidic chemiluminescence immunoassay detection device in this utility model embodiment 1
On the structural representation of 12 miniflow detector units is installed；
Fig. 2 is the enlarged diagram of single miniflow detector unit on micro-fluidic disc in Fig. 1；
Fig. 3 is the micro-fluidic disc of a kind of micro-fluidic chemiluminescence immunoassay detection device in this utility model embodiment 2
On the structural representation of 4 miniflow detector units is installed；
Fig. 4 is the enlarged diagram of single miniflow detector unit on micro-fluidic disc in Fig. 3.
Detailed description of the invention
Embodiment 1
The one micro-fluidic chemiluminescence immunoassay detection device of the present embodiment, as shown in Figure 1, 2, including micro-fluidic disc
13, described micro-fluidic disc 13 is provided with 12 miniflow detector units, (belonging to the operation of simple pointer many person-portions)；Described is micro-
Stream detector unit includes that whole blood injects groove 1, and described whole blood injects groove 1 by whole blood separation channel 2 and blood cell accumulator tank 3 phase
Even, described blood cell accumulator tank 3 connects with blood plasma transmission runner 4；Described blood plasma transmits runner 4 and mixes/detect groove 7 top phase
Even；Described mixing/detecting groove 7 top is connected by pipeline and the first reagent accumulator tank 9；Described mixing/detecting groove 7 top
Connect also by pipeline and the 3rd reagent accumulator tank 10；Described mixing/detecting groove 7 side is provided with gas compression release groove 8；
Connected by pipeline and the first waste liquid tank 6 bottom described mixing/detecting groove 7, and with the bottom described mixing/detecting groove 7
First valve 5 is installed on the pipeline between one waste liquid tank 6；Pipeline and second is passed through at the first described reagent accumulator tank 9 top
The bottom connection of reagent accumulator tank 11；The 3rd described reagent accumulator tank 10 is by pipeline and the bottom of the 4th reagent accumulator tank 12
Connection.
The present embodiment is suitable for the chemiluminescence immunoassay detection of the many person-portions of simple pointer, as a example by c reactive protein detects, by whole blood
Injection groove 1 injects whole blood 60 microlitre, the first reagent accumulator tank 9 injects immunity microparticle and (engaged seizure on microparticle anti-
Body) 30 microlitres, second reagent accumulator tank 11 inject detecting antibody 26 microlitre.With rotating speed 5,000 RPM (acceleration a=
10,000 RPM/s) manipulate micro-fluidic disc 13 and rotate 90 seconds, can be at whole blood separation channel 2 separated plasma and blood cell, and will
Immunity microparticle and detecting antibody are sent to mixing/detecting groove 7, and immunity microparticle can remain in mixing/detecting by mat the first gate 5
In groove 7.With rotating speed 1,170 RPM (acceleration a=950 RPM/s) manipulate micro-fluidic disc 13 and rotate 20 seconds, can be by 20
The blood plasma of microlitre transmits runner 4 via blood plasma and is sent to mixing/detecting groove 7, and blood cell is then retained in blood cell accumulator tank 3.Now,
Owing to centrifugal force is more than gas pressure, can be by gas compression in gas compression release groove 8.With rotating speed 250 RPM (acceleration
A=2,300 RPM/s) manipulate micro-fluidic disc 13 and rotate 30 seconds, can gas be discharged (centrifugal from gas compression release groove 8
Power is less than gas pressure), gas release can produce bubble, can be used for disturbance and mixes/detect the liquid in groove 7, and then reaches mixed
The effect closed/cultivate.Cleanout fluid 60 microlitre is injected, with rotating speed Isosorbide-5-Nitrae 20 RPM (acceleration a=in the 3rd reagent accumulator tank 10
3,550 RPM/s) manipulate micro-fluidic disc 13 and rotate 25 seconds, cleanout fluid can be sent to mixing/detecting groove 7, cleanout fluid can
Liquid in displacement mixing/detecting groove 7, this liquid can be transferred into the first waste liquid tank 6, and immunity microparticle can be by the first valve
5 designs, are remained in mixing/detecting groove 7.Luminous substrate 40 microlitre is injected in the 4th reagent accumulator tank 12, and to turn
Speed 1,800 RPM (acceleration a=550 RPM/s) manipulates micro-fluidic disc 13 and rotates 35 seconds, can be sent to mix by substrate
Closing/detecting groove 7, the liquid in the replaceable mixing of substrate/detecting groove 7, this liquid can be transferred into the first waste liquid tank 6, and immunity is micro-
Granule can design by the first valve 5, is remained in mixing/detecting groove 7.Finally, the liquid reacted can mix/detect
Survey groove 7 to detect.
The one micro-fluidic chemiluminescence immunoassay detection device of the present embodiment and using method thereof have efficient, stable, simple
Just feature, can reach whole blood by this utility model with whole blood sample direct injected (being not required to extra Sample pretreatment)
Separation, quantitative blood plasma transmission, mixing (cultivation), the immunoreation step of cleaning.
Embodiment 2
The one micro-fluidic chemiluminescence immunoassay detection device of the present embodiment, as shown in Figure 3,4, including micro-fluidic disc
13, described micro-fluidic disc 13 is provided with 4 miniflow detector units, (each detector unit can complete three immunity simultaneously
Index, can carry out at least 4 pattern detection simultaneously, belongs to single part little set meal unit) described in miniflow detector unit include entirely
Blood injects groove 1, and described whole blood injects groove 1 and is connected with blood cell accumulator tank 3, and described blood cell accumulator tank 3 and blood plasma transmit runner 4 even
Logical；Described blood plasma transmits runner 4 and is connected with mixing/detecting groove 7 top；Described mixing/detecting groove 7 top by pipeline with
First reagent accumulator tank 9 connects；Described mixing/detecting groove 7 top connects also by pipeline and the 3rd reagent accumulator tank 10；Institute
The mixing stated/detecting groove 7 side is provided with gas compression release groove 8；By pipeline and the bottom described mixing/detecting groove 7
One waste liquid tank 6 connects, and is provided with the first valve on the pipeline bottom described mixing/detecting groove 7 and between the first waste liquid tank 6
5；The first described reagent accumulator tank 9 top is connected by the bottom of pipeline and the second reagent accumulator tank 11；The 3rd described examination
Agent accumulator tank 10 is connected by the bottom of pipeline and the 4th reagent accumulator tank 12.Described blood plasma transmits runner 4 and mixes/detect
Groove 7 is also equipped with blood plasma quantitative slot 14 between top；The first through runner 16 and phase are passed through in described blood plasma quantitative slot 14 top
Blood plasma quantitative slot 14 top connection in adjacent miniflow detector unit；The bottom of the 4th described reagent accumulator tank 12 and the 3rd reagent
Also pass sequentially through the second through runner 20 of through runner the 19, the 4th reagent quantitative groove the 17, the 3rd between accumulator tank 10 to be connected, and institute
The second through runner 19 and the 4th reagent quantitative in adjacent miniflow detector unit are passed through in the 4th reagent quantitative groove 17 top stated
Groove 17 top connects；After connecting with blood plasma quantitative slot 14 bottom the 3rd described reagent accumulator tank 10 again with mix/detect groove 7 and push up
Portion is connected by pipeline, and bottom the 3rd described reagent accumulator tank 10 and also passes sequentially through the 3rd between blood plasma quantitative slot 14 and pass through
Passage flow duct the 20, the 3rd reagent quantitative groove the 18, first through runner 16 is connected；And the 3rd described reagent quantitative groove 18 top passes through
3rd through runner 20 and the 3rd reagent quantitative groove 18 top connection in adjacent miniflow detector unit；The first described through stream
Road the 16, second through runner 20 of through runner the 19, the 3rd connects with the second waste liquid tank 21 respectively.Described blood plasma quantitative slot 14 with
On pipeline between mixing/detecting groove 7, the second valve 15 is installed；The 3rd described reagent quantitative groove 18 and the first through runner
3rd valve 22 is installed on the pipeline between 16；The described pipe between the 4th reagent quantitative groove 17 and the 3rd through runner 20
4th valve 23 is installed on road.
The present embodiment is suitable for the chemiluminescence immunoassay detection of the little set meal of single part, with heart infarction three (TnT, creatines
Kinase isozyme, Myoglobin) as a example by, by whole blood injection, groove 1 injects whole blood 180 microlitre, the first reagent accumulator tank 9 injects
Immunity microparticle (having engaged capture antibodies on microparticle) 25 microlitres, the second reagent accumulator tank 11 inject detecting antibody 32
Microlitre.With rotating speed 5,500 RPM (acceleration a=10,500 RPM/s) manipulate micro-fluidic disc 13 and rotate 110 seconds, can be complete
Separated plasma and blood cell；Manipulate micro-fluidic disc 13 with rotating speed 800 RPM (acceleration a=1,500 RPM/s) and rotate 30
Second, blood plasma can be sent to blood plasma quantitative slot 14 through blood plasma transmission runner 4 and carry out quantitatively.In addition, also can be by micro-for immunity
Granule and detecting antibody are sent to mixing/detecting groove 7 by centrifugal force (disc rotation), and immunity microparticle can mat the first gate 5
(size of gate is less than immune microparticle, so immunity microparticle can be remained in mixing/detecting groove) remaines in mixing/detecting
Groove 7.With rotating speed 1,000 RPM (acceleration a=1,700 RPM/s) manipulates micro-fluidic disc 13 and rotates 20 seconds, can be by fixed
Amount blood plasma (25 microlitre) is stored in blood plasma quantitative slot 14, and unnecessary blood plasma can be transferred into the second waste liquid tank 21(through centrifugal
Unnecessary blood plasma is sent to the second waste liquid tank by power).Manipulate micro-with rotating speed 1,600 RPM (acceleration a=2,700 RPM/s)
Stream sheet 13 of manipulating stock quotations rotates 9 seconds, and the most quantitative blood plasma can break through the second valve 15, and is transferred into mixing/detecting groove 7.To turn
Speed 2,300 RPM (acceleration a=6,700 RPM/s) manipulates micro-fluidic disc 13 and rotates 11 seconds, owing to centrifugal force is more than
Gas pressure, can be by gas compression in gas compression release groove 8.Again with rotating speed 800 RPM (acceleration a=700 RPM/s)
Manipulate micro-fluidic disc 13 to rotate 15 seconds, can by gas from gas compression release groove 8 discharge (centrifugal force be less than gas pressure
Power), gas release can produce bubble, can be used for disturbance and mixes/detect the liquid in groove 7, and then reaches the effect of mixing/cultivation
Really.Cleanout fluid 200 microlitre is injected, with rotating speed 1,050 RPM (acceleration a=3,700 RPM/ by the 3rd reagent accumulator tank 10
S) manipulating micro-fluidic disc 13 to rotate 12 seconds, can be carried out by cleanout fluid quantitatively, unnecessary cleanout fluid can be transferred into second and give up
Liquid bath 21.And with rotating speed 2,350 RPM (acceleration a=1,100 RPM/s) manipulate micro-fluidic disc 13 and rotate 22 seconds,
Quantitative cleanout fluid (55 microlitre) can break through the 3rd valve 22, and is transferred into mixing/detecting groove 7, and cleanout fluid is replaceable mixed
Closing/detect the liquid in groove 7, this liquid can be transferred into the first waste liquid tank 6, and immunity microparticle can design by the first gate 5,
Remained in mixing/detecting groove 7.Luminous substrate 100 microlitre is injected, with rotating speed 950 in the 4th reagent accumulator tank 12
RPM (acceleration a=2,300 RPM/s) manipulates micro-fluidic disc 13 and rotates 15 seconds, can luminous substrate be carried out quantitatively, many
Remaining luminous substrate can be transferred into the second waste liquid tank 21.Manipulate with rotating speed 2,250 RPM (acceleration a=900 RPM/s)
Micro-fluidic disc 13 rotates 20 seconds, and the most quantitative luminous substrate (30 microlitre) can break through the 4th valve 23, and is transferred into
Mixing/detecting groove 7, the liquid in the replaceable mixing of luminous substrate/detecting groove 7, this liquid can be transferred into the first waste liquid tank 6,
Immunity microparticle can design by the first gate 5, is remained in mixing/detecting groove 7.Finally, the liquid reacted can be
Mixing/detecting groove 7 detects.
The one micro-fluidic chemiluminescence immunoassay detection device of the present embodiment and using method thereof have efficient, stable, simple
Just feature, can reach whole blood by this utility model with whole blood sample direct injected (being not required to extra Sample pretreatment)
Separation, quantitative blood plasma transmission, mixing (cultivation), the immunoreation step of cleaning.
Claims (5)
1. a micro-fluidic chemiluminescence immunoassay detection device, it is characterised in that: include micro-fluidic disc (13), described miniflow
Manipulating stock quotations and be provided with miniflow detector unit on sheet (13), described miniflow detector unit includes that whole blood injects groove (1), described whole blood
Injecting groove (1) to be connected with blood cell accumulator tank (3) by whole blood separation channel (2), described blood cell accumulator tank (3) transmits stream with blood plasma
Road (4) connects；Described blood plasma transmits runner (4) and is connected with mixing/detecting groove (7) top；Described mixing/detecting groove (7)
Top is connected by pipeline and the first reagent accumulator tank (9)；Described mixing/detecting groove (7) top is also by pipeline and the 3rd
Reagent accumulator tank (10) connects；Described mixing/detecting groove (7) side is provided with gas compression release groove (8)；Described is mixed
Close/detecting groove (7) bottom passes through pipeline and the first waste liquid tank (6) connects, and described mixing/detecting groove (7) bottom is with first
First valve (5) is installed on the pipeline between waste liquid tank (6)；The first described reagent accumulator tank (9) top by pipeline with
The bottom connection of the second reagent accumulator tank (11)；The 3rd described reagent accumulator tank (10) passes through pipeline and the 4th reagent accumulator tank
(12) bottom connection.
A kind of micro-fluidic chemiluminescence immunoassay the most as claimed in claim 1 detection device, it is characterised in that: described whole blood note
Enter groove (1) to connect with blood cell accumulator tank (3)；Described blood plasma transmits runner (4) also pacifies between groove (7) top with mixing/detecting
Equipped with blood plasma quantitative slot (14)；Described blood plasma quantitative slot (14) top is detected with adjacent miniflow by the first through runner (16)
Blood plasma quantitative slot (14) top connection on unit；The bottom of the 4th described reagent accumulator tank (12) and the 3rd reagent accumulator tank
(10) also pass sequentially through between the second through runner (19), the 4th reagent quantitative groove (17), the 3rd through runner (20) be connected, and
The 4th described reagent quantitative groove (17) top is by the second through runner (19) and the 4th examination in adjacent miniflow detector unit
Agent quantitative slot (17) top connects；Again with mixed after connecting with blood plasma quantitative slot (14) bottom the 3rd described reagent accumulator tank (10)
Close/detecting groove (7) top is connected by pipeline, and described 3rd reagent accumulator tank (10) bottom and blood plasma quantitative slot (14) it
Between also pass sequentially through the 3rd through runner (20), the 3rd reagent quantitative groove (18), the first through runner (16) be connected；And it is described
The 3rd through runner (20) and the 3rd reagent quantitative in adjacent miniflow detector unit are passed through in 3rd reagent quantitative groove (18) top
Groove (18) top connects；Described the first through runner (16), the second through runner (19), the 3rd through runner (20) respectively with
Second waste liquid tank (21) connects.
A kind of micro-fluidic chemiluminescence immunoassay the most as claimed in claim 2 detection device, it is characterised in that: described blood plasma is fixed
Measuring tank (14) is provided with the second valve (15) with on the pipeline mixing/detecting between groove (7)；The 3rd described reagent quantitative groove
(18) on the pipeline and between the first through runner (16), the 3rd valve (22) is installed；The 4th described reagent quantitative groove (17)
And on the pipeline between the 3rd through runner (20), the 4th valve (23) is installed.
A kind of micro-fluidic chemiluminescence immunoassay the most as claimed in claim 1 detection device, it is characterised in that: described is micro-fluidic
The upper miniflow detector unit installing more than 4 of disc (13).
A kind of micro-fluidic chemiluminescence immunoassay the most as claimed in claim 2 detection device, it is characterised in that: described is micro-fluidic
Disc (13) is upper installs 4 or 12 miniflow detector units.
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| CN201620440535.5U CN205749537U (en) | 2016-05-13 | 2016-05-13 | A kind of micro-fluidic chemiluminescence immunoassay detection device |
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| CN201620440535.5U CN205749537U (en) | 2016-05-13 | 2016-05-13 | A kind of micro-fluidic chemiluminescence immunoassay detection device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105842468A (en) * | 2016-05-13 | 2016-08-10 | 绍兴普施康生物科技有限公司 | Microfluidic chemiluminescence immune detection device and use method thereof |
| CN108554466A (en) * | 2018-03-01 | 2018-09-21 | 深圳韦尔达科技合伙企业（有限合伙） | The centrifugal separating device of micro-fluidic chip |
| CN108982824A (en) * | 2018-05-11 | 2018-12-11 | 石家庄禾柏生物技术股份有限公司 | A kind of reagent disc test device |
| CN109030812A (en) * | 2018-07-19 | 2018-12-18 | 东莞东阳光科研发有限公司 | A kind of micro-fluidic chip based on immune detection and biochemistry detection, detector and detection method |
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2016
- 2016-05-13 CN CN201620440535.5U patent/CN205749537U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105842468A (en) * | 2016-05-13 | 2016-08-10 | 绍兴普施康生物科技有限公司 | Microfluidic chemiluminescence immune detection device and use method thereof |
| CN105842468B (en) * | 2016-05-13 | 2017-12-22 | 绍兴普施康生物科技有限公司 | A kind of micro-fluidic chemiluminescence immunoassay detection means and its application method |
| CN108554466A (en) * | 2018-03-01 | 2018-09-21 | 深圳韦尔达科技合伙企业（有限合伙） | The centrifugal separating device of micro-fluidic chip |
| CN108982824A (en) * | 2018-05-11 | 2018-12-11 | 石家庄禾柏生物技术股份有限公司 | A kind of reagent disc test device |
| CN108982824B (en) * | 2018-05-11 | 2023-11-10 | 石家庄禾柏生物技术股份有限公司 | Reagent disk testing device |
| CN109030812A (en) * | 2018-07-19 | 2018-12-18 | 东莞东阳光科研发有限公司 | A kind of micro-fluidic chip based on immune detection and biochemistry detection, detector and detection method |
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Address after: Room 408, building C, scientific research building, No. 398, mahuan Road, Lihai Town, Binhai New City, Shaoxing City, Zhejiang Province Patentee after: Zhejiang pushkang Biotechnology Co., Ltd Address before: 312366 Zhejiang city in Shaoxing Province, the coastal city of Shaoxing Ma Huanlu No. 398 Branch Center Patentee before: SHAOXING PUSHKANG BIOTECHNOLOGY Co.,Ltd. |
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