CN205741047U - A kind of centrifugal micro-fluid chip and Pathogen detection system - Google Patents
A kind of centrifugal micro-fluid chip and Pathogen detection system Download PDFInfo
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- CN205741047U CN205741047U CN201620095577.XU CN201620095577U CN205741047U CN 205741047 U CN205741047 U CN 205741047U CN 201620095577 U CN201620095577 U CN 201620095577U CN 205741047 U CN205741047 U CN 205741047U
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Abstract
This utility model relates to microflow control technique application, discloses a kind of centrifugal micro-fluid chip and Pathogen detection system.Centrifugal micro-fluid chip in this utility model includes that bottom plate and top plate, bottom plate and top plate are combined by adhered layer；The upper surface of bottom plate makes several fluid cavitys, connects the passage of fluid cavity and is positioned at the micro-control valve of passage；Adhered layer is provided with dodges mouth at fluid cavity and passage correspondence position；Top plate is provided with liquid filling hole at fluid cavity correspondence position.Centrifugal micro-fluid chip of the present utility model comprises several fluid cavitys, make to expand during detection all to carry out with centrifugal micro-fluid chip with observation procedure, so that amplification and observation procedure need not re-use other equipment, reduce hardware and human cost, be very easy to detection.
Description
Technical field
This utility model relates to microflow control technique application, particularly to a kind of centrifugal micro-fluid chip
And Pathogen detection system.
Background technology
Microflow control technique provides the multiple of monomolecular nucleic acid detection method and realizes platform, is added by micromechanics
Work technology is integrated in the operation of analyzing of biological sample on the chip of several square centimeters, and it is the most popular
Ground is referred to as chip lab (Lab-on-Chip is called for short " LOC "), wherein CD laboratory
(Lab-on-a-disc) concept due to only employ a simple motor just can on light dish type equipment complete
Become the Automated condtrol of miniflow, receive special interest and concern.Because using small being centrifuged of volume
Formula CD laboratory, not only significantly reduces analysis cost, and it is short to detect the time, highly sensitive,
It is easy to carry, leads biochemical analyzer to develop to intelligent and miniaturization direction.Wherein CD is real
Test room chipset use motor to rotate centrifugal force that compact disk equipment produces processes fluid, so be also called from
Core type micro-fluid chip.
Detection method based on isothermal DNA amplification technology is at present for carrying out the reason of clinical Pathogen detection
Thinking means, wherein (Recombinase Polymerase Amplification is called for short recombinase polymeric enzymatic amplification
" RPA ") method is the monomolecular nucleic acid detection method under a kind of room temperature.RPA is highly sensitive, to temperature and
Equipment requirements is low, and testing result can show real-time, is particularly suitable for pathogenic bacteria particularly food
The detection of middle pathogenic bacteria.When prior art uses CD lab-on-a-chip to carry out augmentation detection, expand and observe
Step can only be carried out on other equipment, such as conventional real-time polymerase chain reaction
(Polymerase Chain Reaction is called for short " PCR ") machine, i.e. can not complete institute with an equipment
There is detection process, add hardware and human cost, and bring great inconvenience to detection.
Utility model content
The purpose of this utility model is to provide a kind of centrifugal micro-fluid chip and Pathogen detection system, makes
Must expand and need not re-use other other equipment with observation procedure, reduce hardware and human cost, pole
The earth facilitates detection.
For solving above-mentioned technical problem, embodiment of the present utility model provides a kind of centrifugal microfluid
Chip, comprises bottom plate and top plate, and described bottom plate and described top plate are combined in one by adhered layer
Rise；The upper surface of described bottom plate makes several fluid cavitys, connects the passage of fluid cavity and is positioned at
Micro-control valve in passage；Described adhered layer is provided with dodges mouth at described fluid cavity and described passage correspondence position；
Described top plate is provided with liquid filling hole at described fluid cavity correspondence position.
Of the present utility model embodiment further provides a kind of Pathogen detection system, comprise: above-mentioned centrifugal
Micro-fluid chip, microcontroller, servo motor and optical transmitting set；Wherein, described micro-control is opened when needs
During valve, described microcontroller drives described generating laser to irradiate described micro-control valve；When needs rotate described
During centrifugal micro-fluid chip, described microcontroller drives described servo motor to rotate, described servo motor
Described centrifugal micro-fluid chip is driven to rotate.
In terms of existing technologies, centrifugal micro-fluid chip comprises some this utility model embodiment
Individual fluid cavity so that during detection, amplification and observation procedure all can be carried out at centrifugal micro-fluid chip, can
So that amplification and observation procedure need not re-use other equipment, reduce hardware and human cost, greatly
Facilitate detection.
It addition, several fluid cavitys described be respectively as follows: cracking chamber, amplification chamber, measurement chamber, dilution chamber,
Observation chamber and refuse chamber；Wherein, described cracking chamber connects described amplification chamber by first passage；Described expansion
Increase chamber and connect described measurement chamber and described refuse chamber by second channel；Described measurement chamber passes through third channel
Connect described dilution chamber；Described dilution chamber connects described observation chamber by fourth lane.
It addition, described centrifugal micro-fluid chip also comprises at least one block of metal derby for heating；Described
Metal derby is arranged on the lower surface position corresponding to described amplification chamber of bottom plate.Use metal derby to amplification
Chamber heating can realize constant-temperature amplification.
It addition, described Pathogen detection system also comprises heating control circuit, described heating control circuit connects
Between the metal derby of described microcontroller and described centrifugal micro-fluid chip.Use heating control circuit
Heating-up temperature accurately can be controlled.
It addition, described Pathogen detection system also comprises strip sensor；Described strip sensor is arranged on institute
State the observation intracavity of centrifugal micro-fluid chip.
It addition, described generating laser is laser diode or light emitting diode, can control easily
Valve, or be the heating of amplification chamber as heater.
It addition, described Pathogen detection system also comprises video camera and flash lamp；Described video camera and described sudden strain of a muscle
Light modulation is connected with described microcontroller respectively, and is arranged on above described centrifugal micro-fluid chip, shooting
Machine is for recording the kinestate of above-mentioned centrifugal micro-fluid chip, and flash lamp is used for providing light to video camera
Line.
Accompanying drawing explanation
Fig. 1 is the centrifugal microfluidic chip structure schematic diagram of this utility model the first embodiment；
Fig. 2 is the fluid cavity structural representation of this utility model the first embodiment；
Fig. 3 is the Pathogen detection system structure schematic diagram of this utility model the second embodiment.
Detailed description of the invention
For making the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing pair
Each embodiment of the present utility model is explained in detail.But, those of ordinary skill in the art can
To understand, in each embodiment of this utility model, in order to make reader be more fully understood that the application, this Shen
Please propose many ins and outs.But, even if there is no these ins and outs and based on following embodiment party
The many variations of formula and amendment, it is also possible to realize the application each claim technical scheme required for protection.
First embodiment of the present utility model relates to a kind of centrifugal micro-fluid chip, and this chip comprises the end
Laminate 12 and top plate 15, bottom plate 12 and top plate 15 are combined by adhered layer 13.The end
The upper surface of laminate makes several fluid cavitys, connects the passage of fluid cavity and is positioned at the micro-of passage
Control valve.Adhered layer is provided with at fluid cavity and passage correspondence position and dodges mouth, and top plate is corresponding at fluid cavity
Position is provided with liquid filling hole, and concrete structure is as shown in Figure 1.
Refer to shown in Fig. 2, fluid cavity including but not limited to: cracking chamber 1, amplification chamber 2, measurement chamber 3,
Refuse chamber 4, dilution chamber 5, observation chamber 6.Wherein, cracking chamber 1 is by first passage 8 connection amplification chamber
2；Amplification chamber 2 connects measurement chamber 3 and refuse chamber 4 by second channel 9；Measurement chamber 3 is by threeway
Road 10 connects dilution chamber 5；Dilution chamber 5 is by fourth lane 11 connection observation chamber 6.
It addition, in order to realize constant-temperature amplification, metal derby can be used to the heating of amplification chamber.Specifically,
Centrifugal micro-fluid chip also comprises at least one block of metal derby for heating, and this metal derby is arranged on bottom
The lower surface of plate is corresponding to the position in amplification chamber, say, that heater 7 is arranged on amplification chamber 2
Underface.
Compared with prior art, the centrifugal micro-fluid chip of present embodiment comprises several fluid cavitys,
Make detection time amplification and observation procedure all can carry out with centrifugal micro-fluid chip so that amplification and
Observation procedure need not re-use other equipment, reduces hardware and human cost, is very easy to detection.
The second embodiment of the present utility model relates to a kind of Pathogen detection system, concrete structure such as Fig. 3
Shown in.This Pathogen detection system comprises: microcontroller 17, servo motor 21, optical transmitting set 19 and
The centrifugal micro-fluid chip 30 of one embodiment.Wherein, microcontroller 17 connects respectively and controls light
Emitter 19 and servo motor 21.When micro-control valve opened by needs, microcontroller drives optical transmitting set to shine
Penetrate micro-control valve.Specifically, valve is made up of paraffin, is filled with iron oxide nanoparticles inside paraffin,
Under light source irradiates, nanoparticle absorbance light energy, after heating paraffin wax, paraffin is melted, then valve quilt
Open.When needs rotate centrifugal micro-fluid chip, microcontroller drives described servo motor to rotate,
Servo motor drives centrifugal micro-fluid chip to rotate.It is to say, in test process, microcontroller 17
Controlled the rotating speed of centrifugal micro-fluid chip 30 by servo motor 21, produce being centrifuged required for testing
Power.
Additionally, what deserves to be explained is, in order to realize that amplification chamber is heated, the pathogenic bacteria of present embodiment
Detecting system also comprises heating control circuit, and heating control circuit is connected to microcontroller and centrifugal miniflow
Between the metal derby of body chip, it is achieved the accurate control to heating-up temperature.It addition, optical transmitting set 19 is permissible
Being laser diode, it is also possible to be light emitting diode, optical transmitting set 19 accepts microcontroller 17 and instructs,
On the one hand can be used to open or close the micro-control valve in passage between each fluid cavity, on the other hand can also
It is used for irradiating amplification chamber, provides the temperature required for isothermal duplication method for amplification chamber 2.It addition, system is also
Comprise video camera 16 and flash lamp 18, video camera and flash lamp are connected with microcontroller respectively, and install
Above centrifugal micro-fluid chip, video camera 16 is for recording above-mentioned centrifugal micro-fluid chip 30
Kinestate, flash lamp 18 provides the light of abundance to video camera 16.
The Pathogen detection system specific works process of present embodiment is as follows:
1, before testing, first strip sensor 22 (as shown in Figure 1) is arranged on centrifugal declining
In the observation chamber 6 of fluid chip, then bottom plate and top plate are pasted together.
2, by the liquid filling hole of top plate, it would be desirable to the lysate of test introduces cracking chamber 1.Splitting of cell
Solution can be irradiated cracking chamber by optical transmitting set and heated, it is also possible to refinement cellular lysate inside cracking chamber
Liquid completes, or the two is carried out simultaneously.
3, after having cracked, control optical transmitting set and irradiate the micro-control valve in first passage 8 so that first
Passage is opened, and drives motor to drive centrifugal micro-fluid chip so that the cell pyrolysis liquid of specified amount enters
Amplification chamber 2.
4, control heating control circuit and transmit the temperature required for isothermal duplication method by metal derby to amplification chamber 2
Degree, carries out amplification step.
5, cell pyrolysis liquid is after amplification chamber 2 completes RPA amplification, controls optical transmitting set irradiation second and leads to
Micro-control valve in road 9 so that second channel 9 is opened, drives motor to drive centrifugal micro-fluid chip,
Make to expand chamber 2 and flow out the RPA amplified production entrance measurement chamber 3 of specified amount, after filling measurement chamber 3,
The RPA amplified production of excess is directly injected into refuse chamber 4；
6, control optical transmitting set and irradiate the micro-control valve in third channel 10, open third channel 10, RPA
Amplified production enters dilution chamber 5 from measurement chamber 3 and carries out mixed diluting.
7, after mixed diluting completes, control optical transmitting set and irradiate the micro-control valve in fourth lane 11, open
Opening fourth lane 11, the RPA amplified production that mixed diluting is crossed enters observation chamber 6 and is observed.
It will be understood by those skilled in the art that the respective embodiments described above be realize of the present utility model
Specific embodiment, and in actual applications, can to it, various changes can be made in the form and details, and
Without departing from spirit and scope of the present utility model.
Claims (8)
1. a centrifugal micro-fluid chip, it is characterised in that comprise bottom plate and top plate, described bottom plate and described top plate are combined by adhered layer；
The upper surface of described bottom plate makes several fluid cavitys, connects the passage of fluid cavity and is positioned at the micro-control valve of passage；
Described adhered layer is provided with dodges mouth at described fluid cavity and described passage correspondence position；Described top plate is provided with liquid filling hole at described fluid cavity correspondence position.
Centrifugal micro-fluid chip the most according to claim 1, it is characterised in that several fluid cavitys described are respectively as follows: cracking chamber, amplification chamber, measurement chamber, dilution chamber, observation chamber and refuse chamber；
Wherein, described cracking chamber connects described amplification chamber by first passage；Described amplification chamber connects described measurement chamber and described refuse chamber by second channel；Described measurement chamber connects described dilution chamber by third channel；Described dilution chamber connects described observation chamber by fourth lane.
Centrifugal micro-fluid chip the most according to claim 2, it is characterised in that described centrifugal micro-fluid chip also comprises at least one block of metal derby for heating；
Described metal derby is arranged on the lower surface position corresponding to described amplification chamber of bottom plate.
4. a Pathogen detection system, it is characterised in that comprise: the centrifugal micro-fluid chip as described in any one in claim 1 to claim 3, microcontroller, servo motor and optical transmitting set；
Wherein, when described micro-control valve opened by needs, described microcontroller drives described optical transmitting set to irradiate described micro-control valve；
When needs rotate described centrifugal micro-fluid chip, described microcontroller drives described servo motor to rotate, and described servo motor drives described centrifugal micro-fluid chip to rotate.
Pathogen detection system the most according to claim 4, it is characterised in that described Pathogen detection system also comprises heating control circuit, described heating control circuit is connected between the metal derby of described microcontroller and described centrifugal micro-fluid chip.
Pathogen detection system the most according to claim 4, it is characterised in that described Pathogen detection system also comprises strip sensor；
Described strip sensor is arranged on the observation intracavity of described centrifugal micro-fluid chip.
Pathogen detection system the most according to claim 4, it is characterised in that described optical transmitting set is laser diode or light emitting diode.
Pathogen detection system the most according to claim 4, it is characterised in that described Pathogen detection system also comprises video camera and flash lamp；Described video camera and described flash lamp are connected with described microcontroller respectively, and are arranged on above described centrifugal micro-fluid chip.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620095577.XU CN205741047U (en) | 2016-01-31 | 2016-01-31 | A kind of centrifugal micro-fluid chip and Pathogen detection system |
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| CN201620095577.XU CN205741047U (en) | 2016-01-31 | 2016-01-31 | A kind of centrifugal micro-fluid chip and Pathogen detection system |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058063A (en) * | 2017-06-12 | 2017-08-18 | 博奥生物集团有限公司 | A kind of method for multiple nucleic acid amplified production fluoroscopic examination based on micro-fluidic chip |
| CN108277149A (en) * | 2018-06-04 | 2018-07-13 | 上海速创诊断产品有限公司 | Nucleic acid detection apparatus, method and system |
| CN108642140A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection method |
| CN108642141A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection reagent mixing adding set |
| CN108642569A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection chip |
| CN108823089A (en) * | 2018-06-07 | 2018-11-16 | 国家纳米科学中心 | Nucleic acid detection chip based on multiple nucleic acids reaction |
| CN108823088A (en) * | 2018-06-07 | 2018-11-16 | 国家纳米科学中心 | Nucleic acid detection apparatus based on micro-fluidic chip |
| CN110205236A (en) * | 2019-06-12 | 2019-09-06 | 中国检验检疫科学研究院 | A kind of paper micro-fluidic chip quickly detecting nucleic acid based on RPA technology |
| CN111774111A (en) * | 2020-07-10 | 2020-10-16 | 上海佰芯生物科技有限公司 | Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof |
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2016
- 2016-01-31 CN CN201620095577.XU patent/CN205741047U/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058063A (en) * | 2017-06-12 | 2017-08-18 | 博奥生物集团有限公司 | A kind of method for multiple nucleic acid amplified production fluoroscopic examination based on micro-fluidic chip |
| CN107058063B (en) * | 2017-06-12 | 2019-08-20 | 博奥生物集团有限公司 | A method of the fluorescence detection of multiple nucleic acid amplified production is used for based on micro-fluidic chip |
| CN108277149A (en) * | 2018-06-04 | 2018-07-13 | 上海速创诊断产品有限公司 | Nucleic acid detection apparatus, method and system |
| CN108277149B (en) * | 2018-06-04 | 2018-09-25 | 上海速创诊断产品有限公司 | Nucleic acid detection apparatus, method and system |
| CN108642141A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection reagent mixing adding set |
| CN108642569A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection chip |
| CN108823089A (en) * | 2018-06-07 | 2018-11-16 | 国家纳米科学中心 | Nucleic acid detection chip based on multiple nucleic acids reaction |
| CN108823088A (en) * | 2018-06-07 | 2018-11-16 | 国家纳米科学中心 | Nucleic acid detection apparatus based on micro-fluidic chip |
| CN108642140A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection method |
| CN108642141B (en) * | 2018-06-07 | 2021-09-10 | 国家纳米科学中心 | Nucleic acid detection reagent mixing and adding device |
| CN108823089B (en) * | 2018-06-07 | 2021-10-01 | 国家纳米科学中心 | Nucleic acid detection chip based on multiple nucleic acid reactions |
| CN108823088B (en) * | 2018-06-07 | 2021-10-01 | 国家纳米科学中心 | Nucleic acid detection device based on micro-fluidic chip |
| CN108642140B (en) * | 2018-06-07 | 2022-06-21 | 国家纳米科学中心 | Nucleic acid detection method |
| CN110205236A (en) * | 2019-06-12 | 2019-09-06 | 中国检验检疫科学研究院 | A kind of paper micro-fluidic chip quickly detecting nucleic acid based on RPA technology |
| CN111774111A (en) * | 2020-07-10 | 2020-10-16 | 上海佰芯生物科技有限公司 | Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof |
| CN111774111B (en) * | 2020-07-10 | 2023-04-11 | 上海佰芯生物科技有限公司 | Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof |
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Granted publication date: 20161130 Termination date: 20180131 |