CN205730460U - Radix Notoginseng total arasaponins column chromatography procedure automatic control system - Google Patents
Radix Notoginseng total arasaponins column chromatography procedure automatic control system Download PDFInfo
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- CN205730460U CN205730460U CN201620387863.3U CN201620387863U CN205730460U CN 205730460 U CN205730460 U CN 205730460U CN 201620387863 U CN201620387863 U CN 201620387863U CN 205730460 U CN205730460 U CN 205730460U
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Abstract
This utility model relates to a kind of Radix Notoginseng total arasaponins column chromatography procedure automatic control system, described automatic control system includes controlling computer, the pump being connected with described control computer, the chromatographic column being connected with described pump, the detector being connected with described chromatographic column and described control computer.This utility model can realize automatically controlling Radix Notoginseng total arasaponins column chromatography elution process, " quantitative elution " and precise controlling, improves the batch-to-batch consistency of Radix Notoginseng total arasaponins intermediate, and beneficially elution process energy-saving consumption-reducing, increases economic efficiency.
Description
Technical field
This utility model relates to Chinese medicine preparation column chromatography technical process control field, is specifically related to Radix Notoginseng total arasaponins
Column chromatography procedure automatic control system.
Background technology
Radix Notoginseng is dry root and the rhizome of panax araliaceae plant Panax notoginseng (Burk) F.H.Chen,
There is dissipating blood stasis hemostasis, effect of subduing swelling and relieving pain, be a kind of important common Chinese medicine, gone up a century in China
Use history.Wherein, the saponin component of Radix Notoginseng is considered as its Chinese drugs.With the total soap of Radix Notoginseng
Glycosides is the traditional medicine Injectio of raw material, such as XUESAITONG (lyophilizing) etc., is Chinese medicine circle " cookle ".
Radix Notoginseng monomer saponin composition is mostly dammarane type (dammarane) tetracyclic triterpene saponin, can be divided into two big classes,
It is 20 (S)-protopanaxadiol-type [20 (S)-protopanaxadiol] and 20 (S)-Protopanaxatriol types respectively
[20(S)-protopanaxatriol].Wherein arasaponin R1, ginsenoside Rg1, ginsenoside Re, Radix Ginseng soap
Glycosides Rb1 and ginsenoside Rd are saponin components main in Radix Notoginseng, are proved to be Radix Notoginseng and the anti-cardiac muscle of preparation thereof
The main pharmacodynamics material of ischemia, is Radix Notoginseng and the index components of quality of the pharmaceutical preparations control thereof.
Chinese medicine macroporous adsorption resin chromatography technique is a kind of common process for refining, and it is used for Radix Notoginseng total arasaponins
In the subtractive process of ejection preparation.Macroporous adsorption resin chromatography technique generally comprises loading, rinses and eluting three
Individual step.Different components absorbability on resin column differs, along with layer is flowed out in flowing the most in order
Analysis post.
At present, the production process of Radix Notoginseng class Chinese medicine preparation is the most extensive, almost without Process Quality Monitoring.Greatly
Elution step during the resin chromatography of hole have impact on content and the ratio of active ingredient in chromatography product, whole
Individual Radix Notoginseng total arasaponins injection manufacture process has critical role.Radix Notoginseng total arasaponins column chromatography elution process is entered
Row modeling, may be implemented on the premise of meeting prescription, with energy-conserving and environment-protective as target, to eluting
Process is optimized.
Utility model content
The purpose of this utility model is to provide a kind of Radix Notoginseng total arasaponins column chromatography procedure automatic control system.With
Radix Notoginseng total arasaponins column chromatography elution process is automatically controlled by realization, " quantitative elution " and precise controlling, improves
The batch-to-batch consistency of Radix Notoginseng total arasaponins intermediate, beneficially elution process energy-saving consumption-reducing, increases economic efficiency.
Technical solutions of the utility model are as follows:
A kind of Radix Notoginseng total arasaponins column chromatography procedure automatic control system, including
Control computer,
The pump being connected with described control computer,
The chromatographic column being connected with described pump,
The detector being connected with described chromatographic column and described control computer.
Preferably, described control computer is industrial computer or server or programmable logic controller (PLC).
Preferably, described control computer is connected by wireline communication network or cordless communication network with described pump.
Preferably, described pump is able to receive that control Computer signal, and can perform described elution parameters, to layer
Analysis post carries out eluting.
Preferably, described detector includes sensor, near infrared spectrum detector, ultraviolet spectra detector etc..
Preferably, described detector and described chromatographic column and described control computer by wireline communication network or
Cordless communication network connects.
Preferably, described chromatographic column is macroporous adsorption resin chromatography post.
Preferably, described control computer can be according to elution kinetics model specification elution parameters.
Compared with prior art, the Radix Notoginseng total arasaponins column chromatography procedure automatic control system that this utility model provides,
It is possible not only to the elution process of Radix Notoginseng total arasaponins is monitored automatically, adapts to the big commercial production of the modernization of Chinese medicine
Demand, it is also possible to be predicted during Radix Notoginseng total arasaponins elution processes control, it is achieved Radix Notoginseng total arasaponins
" quantitative elution " and precise controlling, substantially increase the batch-to-batch consistency of Radix Notoginseng total arasaponins intermediate,
Be conducive to elution process energy-saving consumption-reducing, increase economic efficiency.
This utility model, to Radix Notoginseng total arasaponins column chromatography procedure autocontrol method, comprises the following steps:
1) control computer is according to chromatographic column eluting goal setting elution parameters, and is sent to pump；
2) described pump performs described elution parameters, and chromatographic column is carried out eluting；
3) arasaponin R1 in detector detection eluent, ginsenoside Rg1, ginsenoside Re, Radix Ginseng
Saponin Rb1 and the content of ginsenoside Rd, and testing result is transferred in described control computer；
4) described control computer is according to step 3) testing result formulate new elution parameters, and be sent to
Described pump；Then step 2 is performed) and subsequent step, until eluting completes.
Preferably, described eluting target is arasaponin R1 in eluent, ginsenoside Rg1, ginsenoside
The accumulation elution amount of Re, ginsenoside Rb1 and ginsenoside Rd reaches desired value.
Preferably, described elution parameters includes eluting vehicle ratios, elution flow rate and elution volume etc.；
Preferably, described pump is able to receive that control Computer signal, and can perform described elution parameters, to layer
Analysis post carries out eluting.
Preferably, described detector includes sensor, near infrared spectrum detector, ultraviolet spectra detector etc..
Preferably, described control computer is according to elution kinetics model specification elution parameters；
Preferably, described elution kinetics model passes through BP neural network, uses classical 3-tier architecture
Tissue, uses classical 3-tier architecture tissue, and neural network topology structure is 3-6-5；Input layer is defeated by 3
Enter variable composition, respectively eluting vehicle ratios, elution flow rate and elution volume；Output layer is in eluent
Arasaponin R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd contain
Amount；Hidden layer each layer transmission function is sigmoid function, and the transmission function of output layer neuron is linear function
purclin。
Notoginseng preparations described in the utility model include XUESAITONG (lyophilizing), XUESAITONG JIAONANG, XUESHUANTONG ZHUSHEYE,
XUESHUANTONG JIAONANG.
This utility model Radix Notoginseng total arasaponins column chromatography procedure automatic control system, by building column chromatography eluent
Middle saponin concentrations and the correlation model of elution parameters process conditions, and the cost consumption with elution process is minimum
Process optimization target, reaches intended eluting requirement, adjustment column chromatographic elution process conditions simultaneously.This practicality
Novel improve XUESAITONG (lyophilizing) and Radix Notoginseng class Chinese medicine preparation finished product quality conformance and extract procedure section
Consumption can be subtracted, can promote the use of in the column chromatography production process of XUESAITONG (lyophilizing) and Radix Notoginseng class Chinese medicine preparation.
This utility model is possible not only to automatically monitor the elution process of Radix Notoginseng total arasaponins, adapts to the modernization of Chinese medicine
Big industrial demand, it is also possible to be predicted controlling during Radix Notoginseng total arasaponins elution processes, it is achieved
" quantitative elution " and the precise controlling of Radix Notoginseng total arasaponins, substantially increases the batch of Radix Notoginseng total arasaponins intermediate
Between concordance, beneficially elution process energy-saving consumption-reducing, increases economic efficiency.
Accompanying drawing explanation
Fig. 1 is this utility model Radix Notoginseng total arasaponins column chromatography procedure automatic control system schematic diagram；
In Fig. 1: 1, control computer；2, pump；3, chromatographic column；4, detector.
Fig. 2 is embodiment 2 neural network model result figure.
Fig. 3 is embodiment 3 Radix Notoginseng total arasaponins column chromatography procedure response surface design figure.
Detailed description of the invention
Following example are used for illustrating this utility model, but are not limited to scope of the present utility model.Implement
Unreceipted concrete technology or condition person in example, according to the technology described by the document in this area or condition, or
Person is carried out according to product description.Agents useful for same or instrument unreceipted production firm person, being can be by regular
The conventional products that distributor is commercially available.
Embodiment 1
Radix Notoginseng total arasaponins column chromatography procedure automatic control system, as it is shown in figure 1, include
Control computer 1,
The pump 2 being connected with described control computer,
The chromatographic column 3 being connected with described pump,
The detector 4 being connected with described chromatographic column and described control computer.
Further, described control computer 1 is industrial computer or server or programmable logic controller (PLC).
Further, described control computer 1 and described pump 2 are by wireline communication network or wireless communication networks
Network connects.
Further, described pump is able to receive that control Computer signal, and can perform described elution parameters, right
Chromatographic column carries out eluting.
Further, described detector 4 includes that sensor, near infrared spectrum detector, ultraviolet spectra detect
Device etc..
Further, described detector 4 passes through cable modem with described chromatographic column 3 and described control computer 1
Communication network or cordless communication network connect.
Further, described chromatographic column 3 is macroporous adsorption resin chromatography post.
This Radix Notoginseng total arasaponins column chromatography procedure automatically controls the work process of eluting, including: control computer root
According to chromatographic column eluting goal setting elution parameters, and it is sent to pump；Described pump performs described elution parameters, right
Chromatographic column carries out eluting；Arasaponin R1 in detector detection eluent, ginsenoside Rg1, ginsenoside
The content of Re, ginsenoside Rb1 and ginsenoside Rd, and testing result is transferred to described control calculating
In machine；Described control computer is according to step 3) testing result formulate new elution parameters, and be sent to institute
State pump；Then step 2 is performed) and subsequent step, until eluting completes.
Further, described eluting target is arasaponin R1 in eluent, ginsenoside Rg1, Radix Ginseng soap
The accumulation elution amount of glycosides Re, ginsenoside Rb1 and ginsenoside Rd reaches desired value.
Further, described elution parameters includes eluting vehicle ratios, elution flow rate and elution volume etc..
Further, described control computer is according to elution kinetics model specification elution parameters.
The method for building up of embodiment 2 elution kinetics model
For setting up five kinds of saponin contents (concentration) and the association of elution parameters process conditions in column chromatography eluent
Model (i.e. elution kinetics model), carries out 12 groups of experiments altogether, and experimental design is as shown in table 1.Macropore is inhaled
Attached resin column height 15cm, internal diameter 2.3cm.Take the sample solution of 80mL, flow velocity 0.5BV/h.Then add
150mL water elution, flow velocity 1BV/h.Then under different technology conditions, carry out eluting, often flow out 0.1BV
Eluent is collected as a sample, membrane filtration, carries out saponin constituent quantitative analysis with HPLC liquid.
Table 1 experimental design table
Using the BP neural net model establishing of 3-tier architecture, neural network topology structure is 3-6-5.Wherein, defeated
Three variablees entering layer are respectively eluting vehicle ratios, elution flow rate and elution volume, and each layer of hidden layer transmits
Function is sigmoid function, and output layer is five kinds of saponin each concentration in eluent.Training set, checking collection and
The ratio cut partition of test set is 75%, 15% and 15%.Modeling result is shown in Fig. 2.
In Fig. 2, Training represents training set；Validation represents checking collection；Test represents test set；
All represents all samples；R represent correlation coefficient, abscissa represent standardization after measured value, vertical coordinate table
Showing the predictive value after standardization, Data represents that data point, Fit represent that the regression line, Y=T represent schematic lines.
Embodiment 3
Radix Notoginseng total arasaponins column chromatography procedure autocontrol method, comprises the following steps:
1) control computer is according to chromatographic column eluting goal setting elution parameters, and is sent to pump；
2) described pump performs described elution parameters, and chromatographic column is carried out eluting；
3) arasaponin R1 in detector detection eluent, ginsenoside Rg1, ginsenoside Re, Radix Ginseng
Saponin Rb1 and the content of ginsenoside Rd, and testing result is transferred in described control computer；
4) described control computer is according to step 3) testing result formulate new elution parameters, and be sent to
Described pump；Then step 2 is performed) and subsequent step, until eluting completes.
Wherein, described eluting target quantitative elution limit be total saponins (arasaponin R1, ginsenoside Rg1,
The content of ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd) 2000 to 2300mg.
Described elution parameters includes eluting vehicle ratios, elution flow rate and elution volume etc.；
Described pump is able to receive that control Computer signal, and can perform described elution parameters, carries out chromatographic column
Eluting.
Described detector includes sensor, near infrared spectrum detector, ultraviolet spectra detector etc..
Described control computer is according to elution kinetics model specification elution parameters.
Owing to when elution flow rate is relatively low, eluting effect is preferable, and time cost accounting is less, therefore by eluting stream
Speed is set to 0.5BV/h.Radix Notoginseng total arasaponins column chromatography procedure response surface design figure is shown in Fig. 3-A.
Fig. 3-B is under the flow velocity of 0.5BV/h, under the conditions of different proportion of ethanol and eluting times amount, total
Accumulation saponin elution amount.Visible along with the increase of proportion of ethanol, required solvent times amount reduces, and working place
Reduce the most accordingly.Total saponins (arasaponin R1, ginsenoside Rg1, ginsenoside Re, ginsenoside
The content of Rb1 and ginsenoside Rd and) eluting in the region of 2000 to 2300mg, i.e. can get target and tire out
Long-pending total saponins.
Although, the most with a general description of the specific embodiments this utility model has been made detailed
Describing, but on the basis of this utility model, can make some modifications or improvements it, this is to art technology
It is apparent from for personnel.Therefore, done on the basis of without departing from this utility model spirit these
Amendment or improvement, belong to the scope that this utility model is claimed.
Claims (8)
1. a Radix Notoginseng total arasaponins column chromatography procedure automatic control system, it is characterised in that include
Control computer,
The pump being connected with described control computer,
The chromatographic column being connected with described pump,
The detector being connected with described chromatographic column and described control computer.
Automatic control system the most according to claim 1, it is characterised in that described control computer is industrial computer or server or programmable logic controller (PLC).
Automatic control system the most according to claim 2, it is characterised in that described control computer is connected by wireline communication network or cordless communication network with described pump.
Automatic control system the most according to claim 1 and 2, it is characterised in that described pump is able to receive that control Computer signal, and can perform elution parameters, and chromatographic column is carried out eluting.
Automatic control system the most according to claim 1 and 2, it is characterised in that described detector includes sensor, near infrared spectrum detector, ultraviolet spectra detector.
Automatic control system the most according to claim 5, it is characterised in that described detector is connected by wireline communication network or cordless communication network with described chromatographic column and described control computer.
Automatic control system the most according to claim 1 and 2, it is characterised in that described chromatographic column is macroporous adsorption resin chromatography post.
Automatic control system the most according to claim 1 and 2, it is characterised in that described control computer can be according to elution kinetics model specification elution parameters.
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| CN201620387863.3U CN205730460U (en) | 2016-05-03 | 2016-05-03 | Radix Notoginseng total arasaponins column chromatography procedure automatic control system |
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| CN201620387863.3U CN205730460U (en) | 2016-05-03 | 2016-05-03 | Radix Notoginseng total arasaponins column chromatography procedure automatic control system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105771316A (en) * | 2016-05-03 | 2016-07-20 | 哈尔滨珍宝制药有限公司 | Automatic control system and method for panax notoginseng saponins column chromatography |
| CN106769362A (en) * | 2017-02-08 | 2017-05-31 | 中国环境科学研究院 | A kind of full-automatic adsorption experimental apparatus |
-
2016
- 2016-05-03 CN CN201620387863.3U patent/CN205730460U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105771316A (en) * | 2016-05-03 | 2016-07-20 | 哈尔滨珍宝制药有限公司 | Automatic control system and method for panax notoginseng saponins column chromatography |
| CN105771316B (en) * | 2016-05-03 | 2017-12-22 | 哈尔滨珍宝制药有限公司 | Arasaponin column chromatography procedure automatic control system and method |
| CN105771316B8 (en) * | 2016-05-03 | 2020-08-04 | 哈尔滨珍宝制药有限公司 | Automatic control system and method for panax notoginseng saponins column chromatography process |
| CN106769362A (en) * | 2017-02-08 | 2017-05-31 | 中国环境科学研究院 | A kind of full-automatic adsorption experimental apparatus |
| CN106769362B (en) * | 2017-02-08 | 2018-06-29 | 中国环境科学研究院 | A kind of full-automatic adsorption experimental apparatus |
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