CN205720252U - High-risk HPV specific proteins mark SABC Quality Control sheet and test kit - Google Patents
High-risk HPV specific proteins mark SABC Quality Control sheet and test kit Download PDFInfo
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- CN205720252U CN205720252U CN201620546139.0U CN201620546139U CN205720252U CN 205720252 U CN205720252 U CN 205720252U CN 201620546139 U CN201620546139 U CN 201620546139U CN 205720252 U CN205720252 U CN 205720252U
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Abstract
This utility model provides high-risk HPV specific proteins mark SABC Quality Control sheet and test kit.Specifically, this utility model Quality Control sheet includes: (i) is for the first Quality Control sheet of SABC detection HPV16 specific proteins mark, described first Quality Control sheet includes the first microscope slide, and the first positive quality control district of being positioned on a first type surface of described first microscope slide and the first negative quality control region, and/or (ii) is for the second Quality Control sheet of SABC detection HPV18 specific proteins mark, described second Quality Control sheet includes the second microscope slide, and the second positive quality control district of being positioned on a first type surface of described second microscope slide and the second negative quality control region.The Quality Control of Quality Control sheet of the present utility model is stable, can be effectively improved cervical cancer SABC accuracy.
Description
Technical field
This utility model belongs to bio-medical technology field, particularly to high-risk HPV specific proteins mark immune group
Change Quality Control sheet and test kit.
Background technology
Cervical cancer is the second largest female malignant of China, is also the tumor that unique cause of disease is clear and definite.Numerous studies prove,
High-risk human mammilla papillomavirus (human papi lloma virus, HPV) is the arch-criminal causing cervical cancer, the cervix uteri of 99.7%
Cancer sample all can detect high-risk HPV DNA.The oncogenic virus of cervical cancer is mainly high-risk HPV16 and HPV18.
Cervical lesions progresses to the result that cervical cancer is high-risk HPV persistent infection, takes around the time of about 10 years, sick
Change process include high-risk HPV persistent infection, Cervical intraepitheliaI neoplasia (cervical intraepitheliumneoplasia,
CIN), cancer in situ, infiltrating carcinoma.In cervical cancer, squamous cell carcinoma (squamous cells carcinoma, SCC) accounts for 95%,
The clinical cure rate I phase, the IV phase was only 10%, therefore before cervical cancer up to 80-90%, II phase 60-70%, III phase 40-50%
Pathological changes includes early diagnosis and the preventing and treating of Cervical intraepitheliaI neoplasia (CIN), to controlling the generation of cervical cancer, development and prognosis tool
Significant.
Ministry of Health of the People's Republic of China issues " cervical cancer diagnosis " middle finger in July, 2011 and goes out diagnosis and should use " three rank
Ladder " program, it may be assumed that cervical exfoliated cell detection, colposcopy, pathological diagnosis, and using pathological diagnosis as clinical goldstandard.But
Owing to the pathology detection of morphology interpretation has the strongest subjectivity, even the U.S. strictly undergone training in pathologist, to palace
The interpretation of neck intraepithelial neoplasia (CIN) also tends to exist inconsistent.And CIN is carried out classification accurately, clinic diagnosis is determined
Plan is most important, therefore increasingly payes attention to the detection means with protein marker as target, i.e. immune group in clinical practice application
Weave chemistry colouring method.
When carrying out immunohistochemical staining, it is necessary to be ensured of the specific reaction of antigen and corresponding antibodies, it is ensured that operation
Process is accurate, therefore will arrange controlled trial group in each process of the test of general recommendations, to the deciphering of coloration result also
Set up on the basis of controlled trial result is set up.Conventional controlled trial includes positive control (containing determined antigen), the moon
Property comparison (without determined antigen), blank (substituting one with buffer to resist) etc., but often due to without appropriate in practical operation
Check sample, or check sample lacks and eliminates controlled trial.
Therefore, this area is stable in the urgent need to exploitation Quality Control, can be effectively improved cervical cancer SABC accuracy, for
High-risk HPV (especially HPV16/18) specific proteins mark SABC Quality Control sheet.
Utility model content
The purpose of this utility model is just to provide that a kind of Quality Control is stable, it is accurate to be effectively improved cervical cancer SABC
Property, for high-risk HPV (especially HPV16/18) specific proteins mark SABC Quality Control sheet.
In first aspect of the present utility model, it is provided that a kind of high-risk HPV specific proteins mark SABC Quality Control
Sheet, described Quality Control sheet includes:
I () is for the first Quality Control sheet of SABC detection HPV16 specific proteins mark, described first Quality Control sheet bag
Include the first microscope slide, and the first positive quality control district of being positioned on a first type surface of described first microscope slide and the first negative matter
Control district, wherein, described first positive quality control district includes HPV16 protein positive tumor cell paraffin section, and described first the moon
Property quality control region includes HPV16 protein negative tumor cell paraffin section；And/or
(ii) for the second Quality Control sheet of SABC detection HPV18 specific proteins mark, described second Quality Control sheet
Including the second microscope slide, and the second positive quality control district of being positioned on a first type surface of described second microscope slide and second negative
Quality control region, wherein, described second positive quality control district includes HPV18 protein positive tumor cell paraffin section, and described second
Negative quality control region includes HPV18 protein negative tumor cell paraffin section.
In another preference, thickness d p1 of the paraffin section in described first positive quality control district and the described first negative matter
Thickness d n1 of the paraffin section in control district is equal or of substantially equal,
In another preference, thickness d p2 of the paraffin section in described second positive quality control district and the described second negative matter
Thickness d n2 of the paraffin section in control district is equal or of substantially equal.
In another preference, thickness d p1 of the paraffin section in described first positive quality control district, the described first negative Quality Control
Thickness d n1 of the paraffin section in district, thickness d p2 of paraffin section in described second positive quality control district and described second negative matter
Thickness d n2 of the paraffin section in control district is 3-5 micron,
It is preferred that dp1, dn1, dp2 and dn2 are 3-4 micron.
In another preference, the area Sp1 of the paraffin section in described first positive quality control district, the described first negative Quality Control
The area Sn1 of the paraffin section in district, the area Sp2 of paraffin section in described second positive quality control district and described second negative matter
The area Sn2 of the paraffin section in control district is 9-40mm2。
It is preferred that Sp1, Sn1, Sp2 and Sn2 are 20-25mm2。
In another preference, described first microscope slide there is the first frosted district and the first smooth glass district, and institute
That states the second microscope slide has the second frosted district and the second smooth glass district.
In another preference, described first microscope slide and described second microscope slide are smooth glass sheet.
In another preference, described first frosted district and described second frosted district are provided with different multiple colors, in order to
Distinguish two special quality control sheets.
In another preference, in described HPV16 protein positive tumor cell paraffin section, including for a kind or 2 kinds
Or the paraffin section of multiple HPV16 protein positive tumor cell.
In another preference, in described HPV18 protein positive tumor cell paraffin section, including for a kind or 2 kinds
Or the paraffin section of multiple HPV18 protein positive tumor cell.
In another preference, the described first negative quality control region and the described second negative quality control region are provided with identical HPV egg
The paraffin section of white negative tumor cells.
In another preference, the described first negative quality control region and the described second negative quality control region HPV protein negative tumor
Cell, including a kind or 2 kinds or the paraffin section of multiple high-risk HPV protein negative tumor cell.
In second aspect of the present utility model, it is provided that a kind of for the SABC high-risk HPV specific proteins mark of detection
The test kit of will thing, described test kit comprises:
One container, and
The described high-risk HPV specific proteins mark in first aspect of the present utility model being positioned in described container is exempted from
Epidemic disease group Quality Control sheet.
In another preference, described container at least includes in described Quality Control sheet.
In another preference, described test kit also includes: screens, and each described screens accommodates a described load
Slide and being installed on described container.
In another preference, described container at least includes a screens.
In should be understood that in the range of this utility model, above-mentioned each technical characteristic of the present utility model and below (as implement
Example) in can be combined with each other between each technical characteristic of specifically describing, thus constitute new or preferred technical scheme.It is limited to
Length, tires out the most one by one at this and states.
Accompanying drawing explanation
Fig. 1 shows the first Quality Control sheet in an example of the present utility model and the structure of the second Quality Control sheet.
Fig. 2 shows the test kit containing the Quality Control sheet in Fig. 1.
Each mark is as follows:
2, the microscope slide of the first Quality Control sheet；
3, the microscope slide of the second Quality Control sheet；
10, box body；
20, HPV16 specific proteins mark SABC Quality Control sheet microscope slide；
21, the first positive quality control district；
22, the first negative quality control region；
23, the first frosted district；
24, the first smooth glass district；
30, HPV18 specific proteins mark SABC Quality Control sheet microscope slide；
31, the second positive quality control district；
32, the second negative quality control region；
33, the second frosted district；
34, the second smooth glass district.
Detailed description of the invention
The present inventor, through extensively in-depth study, develops a kind of specific proteins mark for high-risk HPV first
Will thing SABC Quality Control sheet.This Quality Control sheet carries out exempting from after dyeing in the detection of specific proteins mark SABC with actual
Epidemic disease group sample has the concordance of height, therefore can compare as the Quality Control as immunodiagnosis process, improves detection standard
Really property.Complete this utility model on this basis.
Quality Control sheet
This utility model provides the specific proteins mark SABC Quality Control sheet for high-risk HPV.
In preference, Quality Control sheet of the present utility model includes the first Quality Control sheet and the second Quality Control sheet, wherein the first Quality Control
Sheet is used for HPV16, and the second Quality Control sheet is for HPV18.
In this utility model, a kind of the first Quality Control being typically used for HPV16 specific proteins mark SABC
Sheet comprises:
First microscope slide, is provided with the first positive quality control district and the first negative quality control region on a surface of the first microscope slide；
Wherein, described first positive quality control district is provided with HPV16 protein positive tumor cell paraffin section；
Further, the described first negative quality control region is provided with HPV protein negative tumor cell paraffin section.
Preferably, in the negative quality control region in described first positive quality control district and first, each paraffin section is sealed with wax.
In this utility model, a kind of the second Quality Control being typically used for HPV18 specific proteins mark SABC
Sheet comprises:
Second microscope slide, is provided with the second positive quality control district and the second negative quality control region on a surface of the second microscope slide；
Wherein, described second positive quality control district is provided with HPV18 protein positive tumor cell paraffin section.
Further, the described second negative quality control region is provided with HPV protein negative tumor cell paraffin section.
Preferably, in the negative quality control region in described second positive quality control district and second, each paraffin section is sealed with wax.
In this utility model, the microscope slide being suitable for is not particularly limited, and representative example includes (but being not limited to): glass
Glass sheet, plastic sheet；Preferably smooth glass sheet.
In another preference, described slide surface one or both sides have frosted district.
In another preference, the quality control region of described microscope slide is smooth and transparent.
In another preference, the first Quality Control sheet and the second Quality Control sheet are equipped with the frosted district being positioned at microscope slide, and
Described frosted district is provided with different multiple colors, in order to distinguish two special quality control sheets.
In another preference, in described HPV16 protein positive tumor cell paraffin section, including for a kind or 2
Plant or the paraffin section of multiple HPV16 protein positive tumor cell.
In another preference, in described HPV18 protein positive tumor cell paraffin section, including for a kind or 2
Plant or the paraffin section of multiple HPV18 protein positive tumor cell.
In another preference, the HPV protein negative of the described first negative quality control region and the described second negative quality control region swells
Oncocyte includes a kind or 2 kinds or the paraffin section of multiple high-risk HPV protein negative tumor cell.
In another preference, high-risk HPV16/18 specific proteins mark immunodiagnosis palace of the present utility model
The Quality Control sheet of neck cancer, including: two microscope slides, slide surface has tumor cell paraffin section；Microscope slide is smooth glass
Sheet or while having frosted, at frosted be multiple color in order to distinguish two special quality control sheets, another side is smooth sheet glass；Tumor
Cell is to express the tumor cell of HPV16/18 specific proteins mark and do not express the tumor cell of HPV albumen.
Quality Control sheet of the present utility model solves existing cervical cancer high-risk HPV 16/18 specific proteins mark SABC
The problem lacked without Quality Control comparison or Quality Control check sample during diagnosis, supervisory role is played in the operation to staff.
Test kit
On the other hand, this utility model additionally provides SABC and detects the reagent of high-risk HPV specific proteins mark
Box, described test kit comprises a container, one or more (such as two) microscope slide screens and the above-mentioned height being positioned in container
Danger HPV specific proteins mark SABC Quality Control sheet.
In another preference, described test kit also includes operation instructions.
Detection method
Typically, when using immunohistochemical kit Quality Control sheet of the present utility model to detect, according to detection HPV type
(HPV16 and/or HPV18), selects corresponding Quality Control sheet, coordinates immunohistochemical staining reagent first antibody and supporting DAB
Colour reagent box detects.Supporting DAB colour reagent box consists of the following components: 1) reagent 1: confining liquid；2) reagent 2:
Enzyme di-resists；3) reagent 3:DAB Color Appearance System includes DAB buffer and DAB chromogen.
Generally include following steps:
1, the dewaxing treatment of paraffin section
1) section is placed in 60 degree of incubators 30 minutes；
2) each immersion 10 minutes in dimethylbenzene (1) and (2)；
3) at dehydrated alcohol, 95% ethanol, 85% ethanol, each immersion 5 minutes in 75% ethanol；
4) soak 5 minutes in purified water.
2, antigen retrieval
Putting samples in the hot repair liquid of citric acid, Pressure method 2 minutes, manually venting takes together with repair liquid
Going out, room temperature cools down 30 minutes.
3, immunohistochemical staining
1) sample purified water rinse twice, is immersed in 1 × PBS and washes twice, each 3 minutes；
2) adding 100ul 3%H2O2, the wet box of room temperature is closed 10 minutes；
3) 1 × PBS embathes three times, dry after each 3 minutes and clean, at sample, drip 3 reagent 1, the wet box of room temperature
Close 20 minutes；
4) drying is cleaned, and drips 2 first antibodies, and the wet box of room temperature hatches 60 minutes；
5) 1 × PBS embathes five times, dries and clean after each 3 minutes, drips 2 reagent 2, and the wet box of room temperature hatches 30 minutes；
6) 1 × PBS embathes five times, dries and clean after each 3 minutes, takes out the test kit 3 in test kit and prepares DAB colour developing
Liquid, each microscope slide adds at sample 100ul, room temperature treatment 3 minutes；Distilled water rinse 5 minutes；
7) haematoxylin is redyed 1-2 minute, after tap water is rinsed well, soaks 10 minutes in tap water；
8) at 75% ethanol, 85% ethanol, 95% ethanol, dehydrated alcohol soaks successively 5 minutes, at dimethylbenzene (1) and
(2) each immersion 5 minutes in；
9) neutral gum mounting, basis of microscopic observation result.
Coloration result is explained: when hot spot cell cytoplasm presents brown colouring, core is bluish violet, and negative district cell presents indigo plant
During purple, experimental result is effective, and it is invalid otherwise to test.
Major advantage of the present utility model includes:
A () provides a special quality control sheet, and Quality Control for the detection of cervical cancer HPV16/18 specific proteins mark SABC
Sheet steady sources, supply abundance, and energy well Quality Control, improve the work efficiency of SABC.
B () carries out SABC by the section of the different Quality Control sheets prepared and new production simultaneously, can detect
HPV16/18 specific proteins mark SABC reagent is the most effective.
C () carries out SABC by the section of the Quality Control sheet prepared and new production simultaneously, it is possible to enter operating procedure
Row supervision and inspection.
Below in conjunction with specific embodiment, this utility model is expanded on further.Should be understood that these embodiments are merely to illustrate this
Utility model rather than limit scope of the present utility model.The experimental technique of unreceipted actual conditions in the following example is logical
Often according to normal condition, such as Sambrook et al., molecular cloning: laboratory manual (New York:Cold Spring
Harbor Laboratory Press, 1989) condition described in, or according to the condition proposed by manufacturer.Unless additionally
Illustrating, otherwise percentage ratio and number are percentage by weight and parts by weight.
Material
In embodiment, cell strain is commercially available cell strain:
(human cervical carcinoma cell, containing complete HPV16 [each cell about 600 for Cervical Tumor cell model Ca Ski
Individual copy].Number:CRL-1550TM) be HPV16 protein positive tumor cell,
(human cervical carcinoma cell, HPV DNA, RNA are negative for C-33A cell.Number:HTB-31TM) it is that HPV is negative
Tumor cell；
Hela cell (containing complete HPV18 sequence,Number:CCL-2TM) it is HPV18 protein positive tumor
Cell.
Embodiment 1
Quality Control sheet
1, cell is collected with fixing
Collect the CaSki being in exponential phase, Hela, C-33A cell respectively, use PBS to rinse one time, 2000rpm
After centrifugal 10 minutes, carefully discard supernatant, be slowly added into along tube wall that to fix 2 in 10% neutral formalin of 5 times of its volumes little
Time, 2000rpm is centrifuged 10 minutes the most again, outwells supernatant, and 95% ethanol being slowly added into 5 times of its volumes along tube wall fixes 2
Hour.
2, embed and cut into slices
Wrap cell precipitation with lens paper, put into embedded box and conventionally embed.Section, thickness 3-4 μm,
It is respectively placed in the microscope slide quality control region of correspondence, saves backup under the conditions of cold drying.
In a preferred embodiment, described tumor cell paraffin section size is 9-40mm2, thickness is 3-5 μm.
It is preferred that described tumor cell paraffin section size is 20-25mm2, thickness is 3-4 μm.
Embodiment 2
Test kit
Seeing Fig. 2, box body 10 is cuboid plastic casing, comprises two screens 20 and 30 in box body 10, is respectively used to accommodate
The microscope slide 2 of the first Quality Control sheet shown in Fig. 1 and the microscope slide 3 of the second Quality Control sheet.In the present embodiment, the microscope slide of employing is big
Little is 25 × 75mm.Microscope slide 2 and 3 while being frosted district, another side is smooth glass district.The wherein frosted district of microscope slide 2
For white, the frosted district of microscope slide 3 is blue.
Microscope slide 2 comprises microscope slide the first frosted district 23 and the first smooth glass district 24.The table in the first smooth glass district 24
Bread is containing the first positive quality control district 21 and the first negative quality control region 22.Wherein, first positive quality control district 21 Ski tumor Han Ca is thin
Born of the same parents' paraffin section.
Wave carrier piece 3 comprises microscope slide the second frosted district 33 and the second smooth glass district 34.The table in the second smooth glass district 34
Bread is containing the second positive quality control district 31 and the second negative quality control region 32.Wherein, second positive quality control district 31 tumor cell Han Hela
Paraffin section.
First negative quality control region 22 and the second negative quality control region 32 are all containing C-33A tumor cell paraffin section.
Embodiment 3
Detection application
A. one is provided to fix for formalin, the immunohistochemical staining reagent of paraffin embedding cervical tissue detection the
One antibody and supporting DAB colour reagent box, this test kit consists of the following components: 1) reagent 1: confining liquid；2) reagent 2: enzyme
Di-resists；3) reagent 3:DAB Color Appearance System includes DAB buffer and DAB chromogen.
B. step is implemented:
1, the dewaxing treatment of paraffin section；
1) section is placed in 60 degree of incubators 30 minutes；
2) each immersion 10 minutes in dimethylbenzene (1) and (2)；
3) at dehydrated alcohol, 95% ethanol, 85% ethanol, each immersion 5 minutes in 75% ethanol；
4) soak 5 minutes in purified water.
2, antigen retrieval
Putting samples in the hot repair liquid of citric acid, Pressure method 2 minutes, manually venting takes together with repair liquid
Going out, room temperature cools down 30 minutes.
3, immunohistochemical staining
1) sample purified water rinse twice, is immersed in 1 × PBS and washes twice, each 3 minutes；
2) adding 100ul 3%H2O2, the wet box of room temperature is closed 10 minutes；
3) 1 × PBS embathes three times, dry after each 3 minutes and clean, at sample, drip 3 reagent 1, the wet box of room temperature
Close 20 minutes；
4) drying is cleaned, and drips 2 first antibodies, and the wet box of room temperature hatches 60 minutes；
5) 1 × PBS embathes five times, dries and clean after each 3 minutes, drips 2 reagent 2, and the wet box of room temperature hatches 30 minutes；
6) 1 × PBS embathes five times, dries and clean after each 3 minutes, takes out the test kit 3 in test kit and prepares DAB colour developing
Liquid, each microscope slide adds at sample 100ul, room temperature treatment 3 minutes；Distilled water rinse 5 minutes；
7) haematoxylin is redyed 1-2 minute, after tap water is rinsed well, soaks 10 minutes in tap water；
8) at 75% ethanol, 85% ethanol, 95% ethanol, dehydrated alcohol soaks successively 5 minutes, at dimethylbenzene (1) and
(2) each immersion 5 minutes in；
9) neutral gum mounting, basis of microscopic observation result.
Coloration result is explained: when hot spot cell cytoplasm presents brown colouring, core is bluish violet, and negative district cell presents indigo plant
During purple, experimental result is effective, and it is invalid otherwise to test.
50 groups of samples are detected, when using Quality Control flake products of the present utility model as comparison, it is judged that accuracy rate
Reach 100%.
Same 50 groups are detected, when not using Quality Control flake products of the present utility model as comparison, it is judged that accurately
Rate reaches 90%, False Rate 10%.
The all documents mentioned at this utility model are incorporated as reference the most in this application, just as each document quilt
It is individually recited as with reference to like that.In addition, it is to be understood that after having read above-mentioned teachings of the present utility model, this area skill
This utility model can be made various changes or modifications by art personnel, and these equivalent form of values fall within the application claims equally
Book limited range.
Claims (10)
1. a high-risk HPV specific proteins mark SABC Quality Control sheet, it is characterised in that described Quality Control sheet includes:
I () includes for the first Quality Control sheet of SABC detection HPV16 specific proteins mark, described first Quality Control sheet
One microscope slide, and the first positive quality control district of being positioned on a first type surface of described first microscope slide and the first negative Quality Control
District, wherein, described first positive quality control district includes HPV16 protein positive tumor cell paraffin section, and described first feminine gender
Quality control region includes HPV16 protein negative tumor cell paraffin section；And/or
(ii) for the second Quality Control sheet of SABC detection HPV18 specific proteins mark, described second Quality Control sheet includes
Second microscope slide, and the second positive quality control district of being positioned on a first type surface of described second microscope slide and the second negative Quality Control
District, wherein, described second positive quality control district includes HPV18 protein positive tumor cell paraffin section, and described second feminine gender
Quality control region includes HPV18 protein negative tumor cell paraffin section.
Quality Control sheet the most according to claim 1, it is characterised in that the thickness of the paraffin section in described first positive quality control district
Thickness d n1 of the paraffin section in dp1 and the described first negative quality control region is equal, and/or described second positive quality control district
Thickness d p2 of paraffin section and described second negative quality control region in thickness d n2 of paraffin section be equal.
Quality Control sheet the most according to claim 1, it is characterised in that the thickness of the paraffin section in described first positive quality control district
Thickness d n1 of paraffin section in dp1, the described first negative quality control region, the thickness of the paraffin section in described second positive quality control district
Thickness d n2 of the paraffin section in degree dp2 and the described second negative quality control region is 3-5 micron, and/or the described first positive matter
The area Sn1 of the paraffin section in the area Sp1 of the paraffin section in control district, the described first negative quality control region, described second positive
The area Sn2 of the paraffin section in the area Sp2 of the paraffin section of quality control region and the described second negative quality control region is 9-
40mm2。
Quality Control sheet the most according to claim 1, it is characterised in that described first microscope slide there is the first frosted district and
One smooth glass district, and described second microscope slide have the second frosted district and the second smooth glass district.
Quality Control sheet the most according to claim 4, it is characterised in that described first frosted district and described second frosted district are provided with
Different multiple colors.
Quality Control sheet the most according to claim 1, it is characterised in that cut at described HPV16 protein positive tumor cell paraffin
In sheet, including for a kind or 2 kinds or the paraffin section of multiple HPV16 protein positive tumor cell.
Quality Control sheet the most according to claim 1, it is characterised in that cut at described HPV18 protein positive tumor cell paraffin
In sheet, including for a kind or 2 kinds or the paraffin section of multiple HPV18 protein positive tumor cell.
Quality Control sheet the most according to claim 1, it is characterised in that the described first negative quality control region and the described second negative matter
Control district is provided with the paraffin section of identical HPV protein negative tumor cell.
9. the test kit detecting high-risk HPV specific proteins mark for SABC, it is characterised in that described reagent
Box comprises:
One container, and
It is positioned at high-risk HPV specific proteins mark SABC Quality Control sheet described in the claim 1 in described container.
Test kit the most according to claim 9, it is characterised in that described test kit also includes: screens, and each
Described screens accommodates a described microscope slide and is installed on described container.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620546139.0U CN205720252U (en) | 2016-06-07 | 2016-06-07 | High-risk HPV specific proteins mark SABC Quality Control sheet and test kit |
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|---|---|---|---|
| CN201620546139.0U CN205720252U (en) | 2016-06-07 | 2016-06-07 | High-risk HPV specific proteins mark SABC Quality Control sheet and test kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107621542A (en) * | 2017-08-16 | 2018-01-23 | 上海芯超生物科技有限公司 | SABC slide with internal reference and its preparation method and application |
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2016
- 2016-06-07 CN CN201620546139.0U patent/CN205720252U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107621542A (en) * | 2017-08-16 | 2018-01-23 | 上海芯超生物科技有限公司 | SABC slide with internal reference and its preparation method and application |
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