CN205710771U - A kind of T cell incubator - Google Patents
A kind of T cell incubator Download PDFInfo
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- CN205710771U CN205710771U CN201620343924.6U CN201620343924U CN205710771U CN 205710771 U CN205710771 U CN 205710771U CN 201620343924 U CN201620343924 U CN 201620343924U CN 205710771 U CN205710771 U CN 205710771U
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Abstract
This utility model relates to a kind of T cell incubator, and including an airtight container, described container contents is equipped with more than one magnetic bead particles, and each magnetic bead particles is attached with the antibody for being combined with peripheral blood T cells and natural killer T cells；The top of described container be provided with one for introduce blood and in Y type unidirectional double enter pipe, the bottom of described container is provided with one for unidirectional pair of outlet of discharge opeing；An electric magnet, described electric magnet and the control module of being also sticked on described container outer wall is electrically connected；Described unidirectional pair of inlet tube is all connected with inside described container with unidirectional pair of outlet.The beneficial effects of the utility model are: patient specimen is just isolated from the outside after a tube for transfusion is entered and comes, by the time cultivating after program completes and collect from another one outlet, the cell between patient with patient has no chance to contact and produce possible cross-contamination within same space from each other.
Description
Technical field
This utility model relates to T cell and cultivates field, particularly relates to a kind of T cell incubator.
Background technology
It is a kind of common Cell culture invitro technology that peripheral blood lymphocyte is cultivated, it is directed to the separation of peripheral blood lymphocyte, general employing FICOLL gradient centrifugation is drawn in buffy coat to culture dish, or with the magnetic bead specific adsorption T lymphocyte of absorption CD3 or CD28 antibody in magnetic bead surfaces, then cultivate with in Nostoc commune Vanch ware, draw addition culture fluid in lymphocyte to culture dish with suction pipe to cultivate, this cultivation is to complete in cell cultivation ventilating kitchen (clean bench) of an opening, the pollution of the microorganisms such as the bacterial virus that the big transfer process in space is easily subject in air, it is placed on 37oC cell culture incubator is cultivated.When transfection, need to be shifted out to be put into inside ventilating kitchen to change liquid and add transfection liquid, be put back into 37 the most againoCultivating in C cell culture incubator, centre also needs to again and again take out inside cell culture incubator come clean bench, again from clean bench to cell culture incubator in, until cultivated.Clinically CART cell cultivate do such operation sequence can only next patient, two patients are unallowed in same cell cultivates room, because cannot ensure that the pollution that do not crosses one another cultivated by the hemocyte of two patients, therefore the formality under this open culturing mode of clinical grade other blood lymphocyte cultivation at present is loaded down with trivial details, inefficiency, manpower and materials consume very big, and cost is the highest, it is impossible to large-scale promotion.
Utility model content
The purpose of this utility model is for above weak point, it is provided that a kind of T cell incubator, low cost, it is to avoid cross infection.
The scheme that this utility model solution technical problem is used is: a kind of T cell incubator, it is characterized in that: include an airtight container, described container contents is equipped with more than one magnetic bead particles, and each magnetic bead particles is attached with the antibody for being combined with peripheral blood T cells or natural killer T cells；The top of described container is provided with one for introducing blood and the unidirectional pair of inlet tube in Y type, and the bottom of described container is provided with one for unidirectional pair of outlet of discharge opeing；An electric magnet, described electric magnet and the control module of being also sticked on described container outer wall is electrically connected；Described unidirectional pair of inlet tube is all connected with inside described container with unidirectional pair of outlet.
Further, described unidirectional pair of inlet tube includes blood preparation entrance and the packaging virus transfection liquid entrance being positioned at unidirectional pair of inlet tube top, the bottom of described unidirectional pair of outlet is provided with the first outlet and the second outlet, described first outlet is connected with waste fluid bag, and described second outlet is connected with transfusion bag；Described waste fluid bag is equipped with a small amount of preservative.
Further, the lower end of described unidirectional pair of inlet tube and the upper end of unidirectional pair of outlet are respectively equipped with one first check valve and the second check valve, and described first check valve and the second check valve are all electrically connected with control module.
Further, described antibody is CD3 monoclonal antibody, CD3 polyclonal antibody or CD28 antibody.
Further, the feed tube for inputting normal saline is further respectively had, for input air and the air inlet pipe of the mixed gas of CO2 with for inputting the tube for transfusion of culture fluid on the sidewall of described container, being equipped with one for regulating the regulation valve of input quantity on described feed tube, air inlet pipe and tube for transfusion, each regulation valve is all electrically connected with described control module；Described feed tube, air inlet pipe are connected inside described container with tube for transfusion.
Further, described container is placed in the sealed thermal insulating case of one 37 DEG C, and described sealed thermal insulating case is provided with five through holes passed respectively with described unidirectional pair of inlet tube, the unidirectional pair of outlet, feed tube, air inlet pipe and tube for transfusion corresponding matching.
Further, also including one for supporting the mechanical hand of described electric magnet, described mechanical hand is arranged at below described container and drives through a motor and rotates around described container；Described mechanical hand also moves up and down through a longitudinal driving mechanism.
Compared with prior art, this utility model has following beneficial effect: open the first check valve, blood sample is injected into container in unidirectional pair of inlet tube, receptacle is full of magnetic bead, a diameter of 4.5 microns, surface attachment CD3 monoclonal antibody, with T cell generation association reaction, T cell in Blood of Patients is allowed fully to be combined 30-40 minute (rocking lightly) with magnetic bead surfaces, electric magnet is adsorbed magnetic bead on the surface of container, open the second check valve of container bottom, unconjugated cell is allowed slowly to flow away, with normal saline flushing, afterwards culture fluid is pumped into culture dish to cultivate.
Accompanying drawing explanation
Below in conjunction with the accompanying drawings this utility model patent is further illustrated.
Fig. 1 is the structural representation of the embodiment of the present invention.
Fig. 2 is the inventive embodiments structural representation with couveuse.
In figure: 1-container；2-magnetic bead particles；Unidirectional pair of inlet tube of 3-；Unidirectional pair of outlet of 4-；5-air inlet pipe；6-feed tube；7-tube for transfusion；8-electric magnet；9-the first check valve；10-the second check valve；11-regulates valve；12-couveuse.
Detailed description of the invention
With detailed description of the invention, this utility model is further illustrated below in conjunction with the accompanying drawings.
As shown in Figure 1-2, a kind of T cell incubator of the present embodiment, including an airtight container 1, in described container 1, it is equipped with more than one magnetic bead particles 2, each magnetic bead particles 2 is attached with the antibody for being combined with peripheral blood T cells or natural killer T cells；The top of described container 1 is provided with one for introducing blood and the unidirectional pair of inlet tube 3 in Y type, and the bottom of described container 1 is provided with one for unidirectional pair of outlet 4 of discharge opeing；Also be sticked on described container 1 outer wall an electric magnet 8, and described electric magnet 8 is electrically connected with a control module；Described unidirectional pair of inlet tube 2 is all internal with described container 1 with unidirectional pair of outlet 4 to be connected.
From the foregoing, the beneficial effects of the utility model are: the blood specimen that patient newly extracts processes through anticoagulant and is gently injected into container 1 from unidirectional pair of inlet tube 3, jiggle and allow blood lymphocyte fully contact with magnetic bead mixing, T cell includes that natural killer T cells has CD3 antigen naturally to attract T cell on magnetic bead ball surface (T cell separates) with the CD3 antibodies of magnetic bead surfaces due to surface, non-T cell composition in blood then gently washes away with normal saline, T cell is left in inside the container 1 of integration, adsorb in magnetic bead surfaces growth amplification, after 2-3 days, T cell expand 5 times or above time can do next step transfection.By airtight container 1, complete man-to-man T cell and cultivate, low cost, and cross infection can be avoided.
In the present embodiment, described unidirectional pair of inlet tube 3 includes blood preparation entrance and the packaging virus transfection liquid entrance being positioned at unidirectional pair of inlet tube 3 top, the bottom of described unidirectional pair of outlet 4 is provided with the first outlet and the second outlet, described first outlet is connected with waste fluid bag, and described second outlet is connected with transfusion bag；Described waste fluid bag is equipped with a small amount of preservative.Adding a small amount of preservative inside waste fluid bag, to prevent antibacterial and fungus growth, be clean environment inside room, air to filter, and can not there be dead angle in corner, will be prone to sterilization.
In the present embodiment, described unidirectional double enter the lower end of pipe and the upper end of unidirectional pair of outlet 4 be respectively equipped with one first check valve 9 and the second check valve 10, described first check valve 9 and the second check valve 10 are all electrically connected with control module.First check valve 9 only import but no export, opens when blood specimen inputs, and remaining time closes, and prevents extraneous air from entering；Second check valve 10 only export but no import, opens during discharge opeing, and other times are closed, and prevent extraneous air from entering, and preferably ensures the seal of container 1.
In the present embodiment, described antibody is CD3 monoclonal antibody, CD3 polyclonal antibody or CD28 antibody.CD28 can stick to magnetic bead surfaces simultaneously together with CD3 and play stimulation T cell growth, it is also possible to CD28 fusion protein joins the effect playing stimulating cellular growth in culture fluid.
In the present embodiment, the feed tube 6 for inputting normal saline is further respectively had, for the air inlet pipe 5 of input air and the mixed gas of CO2 with for inputting the tube for transfusion 7 of culture fluid on the sidewall of described container 1, being equipped with one for regulating the regulation valve 11 of input quantity on described feed tube 6, air inlet pipe 5 and tube for transfusion 7, each regulation valve 11 is all electrically connected with described control module；Described feed tube 6, air inlet pipe 5 are connected in described container 1 with tube for transfusion 7.Ratio 95%:5% of the mixed gas of air and CO2, the incoming interface of described feed tube 6, air inlet pipe 5 and tube for transfusion 7 is all aseptic nontoxic easily to enter the pump installation connecing and automatically can controlling influx, regulating input quantity by regulation valve 11, incoming interface goes out can not enter to prevent the thing of outside from sucking inside cell culture fluid and normal saline bottle to go only.
In the present embodiment, described container 1 is placed in the sealed thermal insulating case 12 of one 37 DEG C, and described sealed thermal insulating case 12 is provided with five through holes passed respectively with described unidirectional pair of inlet tube 3, the unidirectional pair of outlet 4, feed tube 6, air inlet pipe 5 and tube for transfusion 7 corresponding matching.
In the present embodiment, also including one for supporting the mechanical hand of described electric magnet 8, described mechanical hand is arranged at below described container 1 and drives through a motor and rotates around described container；Described mechanical hand also moves up and down through a longitudinal driving mechanism.Described mechanical hand can be arranged at lower section or the week side of couveuse 12, is used for rocking container, it is simple to T cell is sufficiently separated.Described longitudinal driving mechanism can be longitudinally disposed screw body.
In sum, a kind of T cell incubator that this utility model provides, take up room little, low cost, it is possible to achieve the integral type of T cell is cultivated, it is to avoid cross infection.
Above-listed preferred embodiment; the purpose of this utility model, technical scheme and advantage are further described; it is it should be understood that; the foregoing is only preferred embodiment of the present utility model; not in order to limit this utility model; all within spirit of the present utility model and principle, any modification, equivalent substitution and improvement etc. made, within should be included in protection domain of the present utility model.
Claims (7)
1. a T cell incubator, it is characterised in that: including an airtight container, described container contents is equipped with more than one magnetic bead particles, and each magnetic bead particles is attached with the antibody for being combined with peripheral blood T cells or natural killer T cells；The top of described container is provided with one for introducing blood and the unidirectional pair of inlet tube in Y type, and the bottom of described container is provided with one for unidirectional pair of outlet of discharge opeing；An electric magnet, described electric magnet and the control module of being also sticked on described container outer wall is electrically connected；Described unidirectional pair of inlet tube is all connected with inside described container with unidirectional pair of outlet.
A kind of T cell incubator the most according to claim 1, it is characterized in that: described unidirectional pair of inlet tube includes being positioned at blood preparation entrance and the packaging virus transfection liquid entrance on unidirectional pair of inlet tube top, the bottom of described unidirectional pair of outlet is provided with the first outlet and the second outlet, described first outlet is connected with waste fluid bag, and described second outlet is connected with transfusion bag；Described waste fluid bag is equipped with a small amount of preservative.
A kind of T cell incubator the most according to claim 2, it is characterized in that: the lower end of described unidirectional pair of inlet tube and the upper end of unidirectional pair of outlet are respectively equipped with one first check valve and the second check valve, described first check valve and the second check valve are all electrically connected with control module.
A kind of T cell incubator the most according to claim 1, it is characterised in that: described antibody is CD3 monoclonal antibody, CD3 polyclonal antibody or CD28 antibody.
A kind of T cell incubator the most according to claim 1, it is characterized in that: further respectively have the feed tube for inputting normal saline on the sidewall of described container, for input air and the air inlet pipe of the mixed gas of CO2 with for inputting the tube for transfusion of culture fluid, being equipped with one for regulating the regulation valve of input quantity on described feed tube, air inlet pipe and tube for transfusion, each regulation valve is all electrically connected with described control module；Described feed tube, air inlet pipe are connected inside described container with tube for transfusion.
A kind of T cell incubator the most according to claim 5, it is characterized in that: described container is placed in the sealed thermal insulating case of one 37 DEG C, described sealed thermal insulating case is provided with five through holes passed respectively with described unidirectional pair of inlet tube, the unidirectional pair of outlet, feed tube, air inlet pipe and tube for transfusion corresponding matching.
A kind of T cell incubator the most according to claim 6, it is characterised in that: also include that one for supporting the mechanical hand of described electric magnet, described mechanical hand is arranged at below described container and drives through a motor and rotates around described container；Described mechanical hand also moves up and down through a longitudinal driving mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620343924.6U CN205710771U (en) | 2016-04-22 | 2016-04-22 | A kind of T cell incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620343924.6U CN205710771U (en) | 2016-04-22 | 2016-04-22 | A kind of T cell incubator |
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| Publication Number | Publication Date |
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| CN205710771U true CN205710771U (en) | 2016-11-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620343924.6U Active CN205710771U (en) | 2016-04-22 | 2016-04-22 | A kind of T cell incubator |
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| CN (1) | CN205710771U (en) |
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2016
- 2016-04-22 CN CN201620343924.6U patent/CN205710771U/en active Active
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