CN205656198U - Use kit of quick test samples bacterium endotoxin content of gel method - Google Patents
Use kit of quick test samples bacterium endotoxin content of gel method Download PDFInfo
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- CN205656198U CN205656198U CN201620458417.7U CN201620458417U CN205656198U CN 205656198 U CN205656198 U CN 205656198U CN 201620458417 U CN201620458417 U CN 201620458417U CN 205656198 U CN205656198 U CN 205656198U
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- gel method
- rifle head
- apyrogeneity
- box body
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Abstract
The utility model discloses an use kit of quick test samples bacterium endotoxin content of gel method, include box body and the lid that can open, the inside fixed plate that is equipped with of box body, the fixed plate on be provided with hole or the recess that the utensil was placed to the several, the test tube support that the utensil includes the reagent bottle, is equipped with the rifle head box of apyrogeneity rifle head, is equipped with 8 reagent pipes, wherein 2 sealed reagent bottles in the kit are equipped with bacterium endotoxin work standard substance, bacterium endotoxin detection water respectively, 8 sealed reagent pipes during the test tube holds in the palm in the kit are tal reagents. The utility model discloses a kit uses simple and convenient easy operation, and sensitivity is high, and validity, accuracy and ageing that the bacterium endogenous toxic material detected have been guaranteed to one -off aseptic apyrogeneity reagent and consumptive material.
Description
Technical field
This utility model relates to a kind of detection test kit, and especially a kind of application gel method quickly detects sample
The test kit of this bacterial endotoxin.
Background technology
Pyrogen is mainly a kind of endotoxin of antibacterial release, and pyrogen enters body blood blood circulation and can cause
A series of untoward reaction such as heating, therefore, injection pyrogen or baterial endotoxin test are to ensure that injection
The important quality index of safety.Abnormal results: clinically when carrying out intravenous drip and infusing in a large number, by
Containing pyrogen in medicinal liquid, patient occurs ague, high heat in 0.5-1h, perspires, swoons, vomits
Etc. symptom, during high heat, body temperature is up to 40 DEG C, and severe patient even can be suffered a shock.
Exothermic reaction: human body is the most sensitive to bacterial endotoxin.Denier (1-5 nanogram/kg body weight)
Endotoxin just can cause body temperature to rise, and exothermic reaction gradually disappeared after the most about 4 hours.During natural infection,
Because of the continuous growth and breeding of gram negative bacteria, simultaneously with successively dead, disengage endotoxin, therefore heating is anti-
Should will continuing to eliminate completely to internal pathogen.
Leukocytoreaction: after bacterial endotoxin enters in host, account for total white blood cells in blood flow
The neutrophilic granulocyte quantity of 60-70% reduces rapidly, this is because cell is moved and adheres to tissue hair
On thin blood vessel.But after 1-2 hour, endotoxin the neutrophil cell releasing factor induced stimulates bone marrow
Discharge neutrophilic granulocyte therein and enter blood flow so that it is quantity dramatically increases, and has the jejune neutrality of part
Granulocyte is also released.The Salmonella typhi of gram negative bacteria be exception, its endotoxin make white carefully
Born of the same parents' sum is minimizing state all the time.Owing to the overwhelming majority is by white in the blood stream of patients of gram positive bacterial infection
Total cellular score all can increase, so doctor is before diagnosis, in order to tentatively distinguish bacterial infection or disease
Toxicity infects, and is often required to chemically examine the blood of patient, leukocyte carries out sum and measures and differential counting.Quilt
The patient that virus infects, its total white blood cells and neutrophilic granulocyte percentage ratio are substantially in range of normal value.
Endotoxin shock: when gram negative pathogenic bacterium mortality in focus or blood flow, discharge
When a large amount of endotoxins enter blood, endotoxemia can occur.A large amount of endotoxins act on the huge of body and bite
Cell, neutrophilic granulocyte, endotheliocyte, platelet, and complement system and blood coagulation system etc., will
Produce interleukin 1,6,8 and tumor necrosis factor α, histamine, five hydroxytryptamine, prostaglandin,
The bioactive substances such as kassinin kinin.These materials act on thin vessels and cause dysfunction to cause microcirculation to hinder
Hindering, clinical manifestation is microcirculation failure, hypotension, anoxia, acidosis etc., then results in patient's shock,
This pathological reaction is called endotoxin shock.
Gel method detection sample endotoxin principle: be to utilize the degenerating cell in limulus blood, through lysate and
Mechanical system promotes cell rupture and extracts a kind of cell solute, can with and the bacterial endotoxin of trace
Forming special gel reaction, the speed of reflection is relevant with endotoxin concns with the firmness degree of gel.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of simplification and operates, and shortens the operating time
Application gel method quickly detect the test kit of sample bacteria endotoxin content.
In order to solve above-mentioned technical problem, the technical solution adopted in the utility model is: a kind of application gel
Method quickly detects the test kit of sample bacteria endotoxin content, including box body and openable lid, at box
Internal portion, equipped with fixed plate, fixed plate is provided with hole or the groove of several placer, described utensil bag
Include 2 reagent bottle sealed, equipped with the rifle head cartridge of apyrogeneity rifle head with equipped with 8 Reagent Tubes sealed
Test tube support；2 seal reagent bottle, be respectively equipped with bacterial endotoxin working standard reagent bottle and
Reagent bottle equipped with detection of bacterial endotoxin water.
Described box body and described lid are connected to that connect or separate, and described lid covers at described box body
On, link with magnetic clasp or viscose glue.
Described fixed plate is sponge fixed plate or foam fixing plate, is removably attached to tray interior.
Outside described apyrogeneity rifle head cartridge, there is packaging bag, keep state aseptic, pyrogen-free.
Have 2-5 to prop up 1ml apyrogeneity rifle head in apyrogeneity rifle head cartridge, 20-30 props up 200ul apyrogeneity rifle head,
The rifle head bore frame in 22-35 hole.
Described reagent bottle is all conical bottom, round bottom or flat aseptic, apyrogeneity bottle；Reagent bottle cap is all
For inward turning or the bottle cap of outward turning.
The described test tube support material equipped with 8 Reagent Tubes is foam or plastic material, each in test tube support
Have 2 "+", 2 "-", 2 " (+) ", the mark of 2 " (-) ", and
8 Reagent Tubes have been installed on corresponding home position, wherein also indicate on 8 Reagent Tubes accordingly "+",
"-", " (+) ", " (-) " mark.
The beneficial effects of the utility model are: easy to use, easily operate, and highly sensitive, disposably
Aseptic apyrogeneity reagent and consumptive material, it is ensured that the effectiveness of antibacterial endogenous toxin detection, accuracy and ageing.
Accompanying drawing explanation
Fig. 1 is the test kit that this utility model application gel method quickly detects sample bacteria endotoxin content
Box body schematic diagram.
Fig. 2 is the test kit that this utility model application gel method quickly detects sample bacteria endotoxin content
Internal structure schematic diagram.
Detailed description of the invention
With detailed description of the invention, this utility model is described in further detail below in conjunction with the accompanying drawings:
As shown in Figure 1, 2, this utility model application gel method quickly detects sample bacteria endotoxin content
Test kit, including box body 1 and openable lid 2, internal equipped with fixed plate 4 at box body 1, Gu
Determine to be provided with on plate hole or the groove of several placer, described utensil include 2 reagent bottle sealed,
Rifle head cartridge 7 equipped with apyrogeneity rifle head and the test tube support 8 equipped with 8 Reagent Tubes sealed；2 sealings
Reagent bottle, be respectively equipped with the reagent bottle 5 of bacterial endotoxin working standard with equipped with bacterial endotoxin
The reagent bottle 6 of detection water.
Described box body 1 and described lid 2 are connected to that connect or separate, and described lid covers described
On box body, link with magnetic clasp or viscose glue.
Described fixed plate 4 is sponge fixed plate or foam fixing plate, is removably attached to box body 1 internal.
Outside described apyrogeneity rifle head cartridge 7, there is packaging bag, keep state aseptic, pyrogen-free.
There is 2-5 to prop up 1ml in apyrogeneity rifle head cartridge 7 and prop up 200ul apyrogeneity rifle without thermal source rifle head, 20-30
Head, the rifle head bore frame in 22-35 hole..
Described reagent bottle is all conical bottom, round bottom or flat aseptic, apyrogeneity bottle；Reagent bottle cap is all
For inward turning or the bottle cap of outward turning.
The described test tube support equipped with 8 Reagent Tubes (8) material is foam or plastic material, test tube
Be respectively arranged with in torr 2 "+", 2 "-", 2 " (+) ", the mark of 2 " (-) ",
And install 8 Reagent Tubes on corresponding home position, wherein also indicated corresponding on 8 Reagent Tubes
"+", "-", " (+) ", " (-) " mark.
Preferably, described box body 1 and described lid 2 are connected to connect, and described lid covers on described box body,
Link by magnetic clasp 3.
The test kit using method that above-mentioned application gel method quickly detects sample bacteria endotoxin content is as follows:
Step one: pre-heated test tubes thermostat to 37 DEG C.Get out sample to be detected.Open examination respectively
Agent box, takes out all reagent in test kit and consumptive material.
Step 2: open respectively equipped with bacterial endotoxin working standard reagent bottle, detection of bacterial endotoxin
With the reagent bottle of water, rifle head cartridge.
Step 3: 1ml micro sample adding appliance installs apyrogeneity rifle head, with 1ml detection of bacterial endotoxin water
Dissolution of bacteria endotoxin working standard, mixing, discard rifle head.
Step 4: open 8 Reagent Tubes equipped with tachypleus amebocyte lysate respectively, 2 "+" in be separately added into 100ul
Bacterial endotoxin standard substance after detection use water, 100ul dilution；It is separately added into 200ul in 2 "-"
Detection water；The antibacterial after 100ul sample to be tested, 100ul dilution it is separately added in 2 " (+) "
Endotoxin standard；It is separately added into 100ul sample to be tested, 100ul detection water in 2 " (-) ",
Often add a not good liquor and change a rifle head, after all adding liquid, screw a lid on, the Reagent Tube in test tube support
It is put in the test tube thermostat of preheating in advance, hatches 1 hour.
Step 5: by taking-up Reagent Tube, the most a turnback after 1 hour, pipe inner gel is indeformable,
It is not positive from tube wall slippage person, be recorded as "+", gel can not keep complete and from tube wall slippage person
For feminine gender, it is recorded as "-".
Step 6: 2 " (-) " Reagent Tube is as examination sample tube, 2 "-" Reagent Tubes are as feminine gender
Control tube, 2 "+" Reagent Tube is as positive control pipe, 2 " (+) " Reagent Tubes are as examination
Sample positive control pipe.
Result judges:
1. negative control 2 is managed, is feminine gender for these 2 pipes of sample, and positive control 2 is managed, for sample originally
Positive control 2 pipe is the positive, then result of determination is negative；
2. negative control 2 pipe is feminine gender, positive control 2 is managed, confession this positive control of sample 2 is managed,
Be the positive for these 2 pipes of sample, then result of determination is positive；
3. remaining situation, it is invalid that result judges, needs to recheck.
In sum, content of the present utility model is not limited in the above embodiments, in same area
Knowledgeable people other reality can be proposed within technological guidance's thought of the present utility model easily
Execute example, but this embodiment is included within the scope of this utility model.
Claims (7)
1. apply gel method quickly to detect a test kit for sample bacteria endotoxin content, including box body
(1) and openable lid (2), it is characterised in that internal equipped with fixed plate (4) at box body (1),
Being provided with hole or the groove of several placer in fixed plate, described utensil includes 2 reagent sealed
Bottle, the rifle head cartridge (7) equipped with apyrogeneity rifle head and the test tube support (8) equipped with 8 Reagent Tubes sealed；
2 reagent bottle sealed, are respectively the reagent bottle (5) equipped with bacterial endotoxin working standard and dress
There is the reagent bottle (6) of detection of bacterial endotoxin water.
Application gel method the most according to claim 1 quickly detects sample bacteria endotoxin content
Test kit, it is characterised in that described box body (1) and described lid (2) are connected to that connect or the most only
Standing, described lid covers on described box body, links with magnetic clasp or viscose glue.
Application gel method the most according to claim 1 quickly detects sample bacteria endotoxin content
Test kit, it is characterised in that described fixed plate (4) is sponge fixed plate or foam fixing plate, removable
It is fixed on box body (1) with unloading internal.
Application gel method the most according to claim 1 quickly detects sample bacteria endotoxin content
Test kit, it is characterised in that outside described apyrogeneity rifle head cartridge (7), there is packaging bag, keep aseptic,
Pyrogen-free state.
Application gel method the most according to claim 1 quickly detects sample bacteria endotoxin content
Test kit, it is characterised in that have 2-5 to prop up 1ml apyrogeneity rifle head, 20-30 in apyrogeneity rifle head cartridge (7)
Prop up 200ul apyrogeneity rifle head, the rifle head bore frame in 22-35 hole.
Application gel method the most according to claim 1 quickly detects sample bacteria endotoxin content
Test kit, it is characterised in that described reagent bottle is all conical bottom, round bottom or flat aseptic, apyrogeneity
Bottle；Reagent bottle cap is all the bottle cap of inward turning or outward turning.
Application gel method the most according to claim 1 quickly detects sample bacteria endotoxin content
Test kit, it is characterised in that the described test tube support equipped with 8 Reagent Tubes (8) material is foam
Or plastic material, test tube support is respectively arranged with 2 "+", 2 "-", 2 " (+) ", 2
8 Reagent Tubes, wherein 8 examinations has been installed in the mark of " (-) ", and corresponding home position
Also indicate on agent pipe corresponding "+", "-", " (+) ", " (-) " mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620458417.7U CN205656198U (en) | 2016-05-19 | 2016-05-19 | Use kit of quick test samples bacterium endotoxin content of gel method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620458417.7U CN205656198U (en) | 2016-05-19 | 2016-05-19 | Use kit of quick test samples bacterium endotoxin content of gel method |
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| Publication Number | Publication Date |
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| CN205656198U true CN205656198U (en) | 2016-10-19 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414706A (en) * | 2018-03-02 | 2018-08-17 | 江西英大生物技术有限公司 | A kind of folic acid detection kit |
-
2016
- 2016-05-19 CN CN201620458417.7U patent/CN205656198U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414706A (en) * | 2018-03-02 | 2018-08-17 | 江西英大生物技术有限公司 | A kind of folic acid detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171226 Address after: 300403 Tianjin Binhai New Area Zhongguancun (Tianjin) trusted industrial park A11 Patentee after: Tianjin Xin Purcell Biological Medicine Technology Co Ltd Address before: 300392 in Tianjin Binhai Huayuan Industrial Zone (outer ring) two Haitai Development Road No. four Building No. 4 Building 402A Patentee before: TIANJIN PURI PURCELL BIOTECHNOLOGY CO., LTD. |
|
| TR01 | Transfer of patent right |