CN205642913U - Realize quantitative seminal fluid translator of collection, schizolysis, three step of application of sample simultaneously - Google Patents
Realize quantitative seminal fluid translator of collection, schizolysis, three step of application of sample simultaneously Download PDFInfo
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- CN205642913U CN205642913U CN201620069329.8U CN201620069329U CN205642913U CN 205642913 U CN205642913 U CN 205642913U CN 201620069329 U CN201620069329 U CN 201620069329U CN 205642913 U CN205642913 U CN 205642913U
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Abstract
The utility model discloses a " realize quantitative seminal fluid translator of collection, schizolysis, three step of application of sample simultaneously " by the cylindrical container body that has sample connection and tail end baffle, seals the lid of sample connection to and the I -shaped two -sided piston that passes the tail end baffle constitutes. The spermatid lysate that has certain volume in the cylindrical container body can release the sperm that needs to detect and mark albumen. The function of its quantitative sample circuit ration application of sample successively contacts drum ladle tail end closing shield both sides through the both ends of controlling two -sided piston and realizes. The utility model discloses the function of getting the semen volume in the colloidal gold method sperm concentration detect reagent box in the existing market two instruments in ware and the seminal fluid schizolysis bottle merges, and convenient to use has not only still successfully solved the technological problem of the unable precise and quantitative application of sample of existing seminal fluid schizolysis bottle, has higher use value.
Description
Technical field
The invention belongs to the kit of external diagnosis reagent, the function that seminal fluid measure device in colloidal gold method sperm concentration detection kit in the market and seminal fluid crack two instruments of bottle is merged, the most easy to use, the most successfully solve existing seminal fluid cracking bottle cannot accurate quantification sample-adding technical barrier, there is higher use value, be specifically related to a kind of quantitative seminal fluid translator simultaneously realizing gathering, crack, be loaded three steps.
Background technology
The present invention relates to a kind of quantitative seminal fluid translator that can simultaneously realize gathering, crack, be loaded three steps, it is mainly used in the measuring of seminal fluid in colloidal gold method sperm concentration detection kit in the market, cracks and to detection card sample-adding, can realize quantitatively shifting semen sample to a step of detection card by this invention.
It is an object of the invention to the supporting use for sperm concentration detection card, be that household type conveniently self sperm concentration tests kit.
A further object of the present invention is the sample-adding amount accurately controlling sampling amount and being added drop-wise on detection card, to obtain testing result accurately.
Another object of the present invention is to reduce the cost of the existing kit in market, and two parts are integrated into the combination of parts.
Another object of the present invention is to simplify the use step having kit on the market, gives user the most more easily experience, the product of such sperm concentration test card can preferably be promoted inside family and comes.
Sperm concentration is the major reason causing male infertility extremely, the up-to-date regulation of World Health Organization (WHO), if the quantity of sperm is less than 15,000,000 in adult male every milliliter seminal fluid, the most referred to as sperm concentration is on the low side or few smart, can directly affect pregnancy rate.The most detecting, by colloidal gold immunity chromatography, the domestic detection kit that sperm concentration is the most up to standard, the method mainly the concentration of perforatorium albumen is carried out sxemiquantitative test so that it is determined that sperm concentration.In order to realize the half-quantitative detection to sperm concentration, the semen liquefaction sample of certain volume must be measured so that it is mix with the lysate of certain proportion volume, after cell cracking and acrosin discharge, again the seminal fluid lysate of certain volume is added drop-wise on detection card, it is achieved immunochromatography detects.
In the existing reagent kit product of existing market, different containers is all used quantitatively to measure and crack the two process to complete seminal fluid, user needs to draw seminal fluid with special measure device according to scale, then seminal fluid is turned and be added in special cracking bottle, the mixed liquor taking the certain volume in cracking bottle after cell cracks is added drop-wise on detection card, operate three steps independent of one another, very inconvenient.Except operation complexity, existing product is that requirement user measures seminal fluid according to scale, and owing to lacking control, its operation itself can bring certain error of sampling, affects the interpretation of result；The more important thing is, existing product realizes the sample-adding to detection card by the certain lysate dripping number of dropping, owing to the volume of drop is affected by flow rate of liquid, and because firmly different and flow rate of liquid is different during different user dropping, the same liquid volume dripping number is caused to have relatively large deviation, severe jamming detection sample-adding amount, reduce further the accuracy of testing result.
The present invention be the problem in order to solve in above-mentioned prior art and make, its object is to provide a kind of seminal fluid translator easy to use, user can be by the single stepping of simple " pull out-mix-push ", without shifting semen sample between different vessels, smoothly complete sampling, crack and be loaded three processes, the more important thing is, this seminal fluid translator can accurately control sampling and the volume of sample-adding, ensure that testing result is not used difference to be affected by user's individuality, improve the reliability of testing result.
Summary of the invention
The purpose of this utility model is the quantitative seminal fluid translator simultaneously realizing gathering, crack, be loaded three steps, it is mainly used in the measuring of seminal fluid in colloidal gold method sperm concentration detection kit in the market, cracks and to detection card sample-adding, can realize quantitatively shifting semen sample to a step of detection card by this invention.
For achieving the above object, this utility model is by the following technical solutions, a kind of quantitative seminal fluid translator simultaneously realizing gathering, crack, be loaded three steps is provided, it is by the cylindrical container body with sample tap and tail end baffle plate, closing the lid of sample tap, the I-shaped double-faced piston through tail end baffle plate is constituted, and translator is built with cell pyrolysis liquid, except the cell pyrolysis liquid with certain volume in cylindrical container body, also with the reserved air assisting mixing.
The quantitative seminal fluid translator provided in technical scheme, comprises the sample tap that one end is prominent stack shell of cylindrical container body further, and the other end of cylindrical container body is the baffle arrangement of hollow out, and middle aperture is connected post by I-shaped double-faced piston and runs through.
The quantitative seminal fluid translator provided in technical scheme, comprise the tail end baffle arrangement with hollow out further, piston is blocked in work space by tail end baffle arrangement shape thicknesses, it is desirable to the aperture in the middle of it can accommodate the connection post of piston to be passed through, and stops piston to be extracted from cylindrical container body.
The quantitative seminal fluid translator provided in technical scheme, comprise with the lid closing sample tap further, lid uses common screw-in version or chimeric mode with the juncture of cylindrical container body, it is ensured that the cell pyrolysis liquid within when lid does not takes off will not flow out or volatilize.
The quantitative seminal fluid translator provided in technical scheme, comprises sample tap further, is designed to the taper of prominent vessel, with thin tube-like opening, it is ensured that when drawing seminal fluid and reverse mixing, lysate will not flow out；Simultaneously for enabling seminal fluid to be fully contacted with lysate, this tubular opening is less than 10 millimeters.
The quantitative seminal fluid translator provided in technical scheme, comprise with I-shaped double-faced piston further, the I-shaped longitudinal section referring to this structure becomes the shape of " big centre, both sides is little ", the piston of its side is positioned at cylindrical container body interior, and piston area and shape are equal to the cross section, inner side of cylindrical container body, and are formed with the inwall of cylindrical container body and accommodate lysate and the closing space of air；The piston of I-shaped double-faced piston opposite side is positioned at outside cylindrical container body tip baffle plate, requirement ensure that it can not be pushed into cylindrical container body interior, facilitate push-and-pull during in order to use, be designed into major axis beyond cross section circle, ellipse, strip outside stack shell.
The quantitative seminal fluid translator provided in technical scheme, comprise I-shaped double-faced piston further, it cannot be extracted from stack shell completely, and the post that connects connecting two pistons in piston runs through the through aperture in the middle of cylindrical container body tip baffle plate, and is stablized by the realization that contacts with aperture.I-shaped double-faced piston both sides successively decide an inner carrier transportable length in cylinder with the moved distance that contacts of cylindrical container body tail end baffle plate, this length is equal to measure the volume of seminal fluid with the product of tangent plane in cylindrical container body, and this volume is also equal to liquid volume when sperm concentration is analyzed to detection card sample-adding.The length of cylindrical container body is longer than I-shaped double-faced piston and connects the length of post, and except accommodating piston and connect the space of post, the headspace in cylindrical container body be enough to accommodate the spermatid lysate of certain volume and air.Translator is built with cell pyrolysis liquid, and the volume of lysate measures volume with the seminal fluid determined by barrel diameter and I-shaped double-faced piston connecting rod and becomes fixed ratio, it is ensured that when I-shaped double-faced piston is released, the sample-adding amount of release adapts to the marginal value that detection card is corresponding.
This utility model is according to technique scheme, designed and developed a kind of " realizing gathering, cracking, be loaded the quantitative seminal fluid translator of three steps " by with sample tap cylindrical container body (in Fig. 1 1) simultaneously, container ends has the tail end baffle plate (in Fig. 1 2) of middle hollow out, close the lid (in Fig. 1 3) of sample tap, and connecting rod is through I-shaped double-faced piston (in Fig. 1 4) composition of tail end baffle plate.Accommodating the cell pyrolysis liquid (in Fig. 1 5) of certain volume and a small amount of unfilled space in vessel, lysate can discharge the marker protein in the spermatid needing detection, and the space not being filled in vessel contributes to the mixing of seminal fluid and lysate.Its quantitative sampling successively contacts the realization of cylindrical container body tail end closed guard both sides with the function of quantitatively sample-adding by controlling the two ends of double-faced piston.Specifically, without the seminal fluid translator used, the inboard piston of its I-shaped double-faced piston and connecting rod all stretch into cylindrical container body interior, and the contact outside of outboard piston and cylindrical container body tail end baffle plate, piston is in the state (referred to as state A is shown in accompanying drawing 2) not pulled out；After user gets out semen sample, take off the lid closing sample tap, sample tap is submerged in semen sample, pull out I-shaped piston to inboard piston and cylindrical container body tail end baffle plate interior side contacts (referred to as state B, see accompanying drawing 2), thus measured the semen sample of design volume in translator；Seminal fluid mixes with internal lysate after entering translator, by reverse mixing, user can promote that seminal fluid cracks；Subsequently, I-shaped double-faced piston, by the loading district of detection card used for sample tap alignment, is pushed back state A by state B by user, it is achieved thereby that quantitatively sample-adding (Fig. 2).It is characterized in that, in order to improve user's ease for use, in the design, the volume of the sample volume of seminal fluid detection backward with seminal fluid cracking card sample-adding is equal, this can be realized by the ratio of the volume of the volume and sampling (or loading) that optimize cylindrical container this cells in vivo lysate, one of preferred version is 500-2000 microlitre for lysate volume in cylinder, measures seminal fluid and sample-adding amount volume is 50-200 microlitre.
Pass through said method, when using seminal fluid translator, user can be by the single stepping of simple " pull out-mix-push ", without shifting semen sample between different vessels, smoothly complete sampling, crack and be loaded three processes, it is often more important that, this seminal fluid translator can accurately control sampling and the volume of sample-adding, ensure that testing result is not used difference to be affected by user's individuality, improve the reliability of testing result.
Compared with prior art, beneficial effects of the present invention at least that.
1, sampling amount and the sample-adding amount for test can be precisely controlled, it is not necessary to calibrated scale, the most do not changed by droplet size when dripping and affected, make testing result the most accurate.
2, simplify the step of operation, allow user without at different Tool Room separate operations, giving more preferable experience, decrease user self operation factors and the detection error brought simultaneously.
3, instead of seminal fluid measure device and seminal fluid cracking two instruments of bottle with a seminal fluid translator, reduce production cost, carry and cost of transportation declines the most therewith.
By this seminal fluid translator, simplify the use step having kit on the market, give user the most more easily experience, promote the product of its auxiliary products sperm concentration test card and preferably can promote inside family and come.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is operational flowchart of the present utility model.
Detailed description of the invention
Embodiment
The baffle plate of embodiment 1 seminal fluid translator and sample tap design
Seminal fluid translator as described in Figure 1, one end of its cylindrical container body is the sample tap of prominent stack shell, the loading after the semen sample drawing liquefaction and cracking；The other end of cylindrical container body is hollow out baffle arrangement, and middle aperture is connected post by I-shaped double-faced piston and runs through.Its tail end is with the baffle arrangement of hollow out, and this baffle arrangement shape thicknesses does not fix requirement, as long as the connection post that the aperture in the middle of it can accommodate piston passes through, and stops piston to be extracted from container。With the lid of closing sample tap, lid can use common screw-in version or chimeric mode etc. with the juncture of cylindrical container body, it is ensured that the cell pyrolysis liquid within when lid does not takes off will not flow out or volatilize.Its vessel side is sample tap, for convenience of sampling, may be designed to taper, it might even be possible to shorter thin tube-like opening, and opening is (diameter is less than 3 millimeters) preferably tiny, it is ensured that when drawing seminal fluid and reverse mixing, lysate will not flow out；Simultaneously for enabling seminal fluid to be fully contacted with lysate, this tubular opening shorter preferably (less than 10 millimeters).
The plunger designs of embodiment 2 seminal fluid translator
Seminal fluid translator as described in Figure 1, with I-shaped double-faced piston, the I-shaped longitudinal section referring to this structure becomes the shape of " big centre, both sides is little ", the piston of its side is positioned at cylindrical container body interior, and piston area and shape are equal to the cross section, inner side of cylindrical container body, and are formed with the inwall of cylindrical container body and accommodate lysate and the closing space of air；The piston of I-shaped double-faced piston opposite side is positioned at outside cylindrical container body tip baffle plate, this side is piston-shaped does not fix requirement, as long as it is internal to ensure that it can not be pushed into container, push-and-pull is facilitated, it is proposed that be designed into major axis beyond cross section circle, ellipse, strip etc. outside stack shell during in order to use.Its I-shaped double-faced piston cannot be extracted from stack shell completely, and the post that connects connecting two pistons in piston runs through the through aperture in the middle of cylindrical container body tip baffle plate, and is stablized by the realization that contacts with aperture.Double-faced piston both sides successively decide an inner carrier transportable length in cylinder with the moved distance that contacts of cylindrical container body tail end baffle plate, this length is equal to measure the volume of seminal fluid with the product of tangent plane in cylindrical container body, and this volume is also equal to liquid volume when sperm concentration is analyzed to detection card sample-adding.The length of cylindrical container body is longer than I-shaped piston and connects the length of post, and except accommodating piston and connect the space of post, the headspace in cylindrical container body be enough to accommodate the spermatid lysate of certain volume and air.
The formula design of embodiment 3 seminal fluid lysate
Seminal fluid translator as described in Figure 1, in cylinder, lysate is the saline solution of non-ionic octoxynol detergent, alternative includes NP40 (Nonidet P40 40) or the Chaps(propane sulfonic acid inner salt of similar concentration of about 0.1%-1%), or Tween20(polysorbas20), or Triton X-100(Triton X-100) etc. the phosphate solution of Nonionic Detergents.The volume of this lysate measures volume with the seminal fluid determined by barrel diameter and I-shaped double-faced piston connecting rod and becomes fixed ratio, it is ensured that institute's extracting extract can fully crack and convenient detection.
The use of embodiment 4 seminal fluid translator
The container gathering, crack, be loaded in the quantitative seminal fluid translator of three steps is realized while the present invention, body shape can be that there is no particular restriction for cylindrical shape, prism tubular etc., as long as have to accommodate and make semen dilution and the volume of cell cracking cracking necessary amounts liquid, and the length being not blocked when having in the pull-out of I-shaped double-faced piston and push.
Wherein, the material of vessel can use the synthetic resin, even glass or pottery such as polyethylene, polypropylene, polrvinyl chloride, for cost consideration, uses polyethylene rigid plastics especially suitable.
Wherein, container end has middle through baffle plate, make I-shaped double-faced piston connect post from middle through from pass, but ensure that piston can not be pulled out completely cylindrical container body.This baffle plate is not limited to tabular, any can play the structure supporting piston and stoping piston to be pulled out completely.
Wherein, instrument inwall should have water logging lubricant nature, prevents seminal fluid from not soaking internal face, causes some retention, it is impossible to uniformly mixes with lysate.Can use for this and either physically or chemically process, normally only use one of them, naturally it is also possible to the two is also used.
Wherein physical method processes and corona such as can be used to put a process, and the condition of Corona discharge Treatment is advisable can obtain the most suitable water logging lubricant nature.It is with suitable Chemical treatment that chemical method processes, and such as available hydrogen lithium oxide processes, and Lithium hydrate treatment conditions can obtain being best suitable for water logging lubricant nature and be advisable.
Wherein, the sample tap of cylindrical container body highlights stack shell, for convenience of sampling, may be designed to taper, it might even be possible to shorter thin tube-like opening, and opening is (diameter is less than 3mm) preferably tiny, it is ensured that when drawing seminal fluid and reverse mixing, lysate will not flow out；Simultaneously for enabling seminal fluid to be fully contacted with lysate, this tubular opening preferably shorter (less than 10mm).
Wherein, the shape of the lid being located at sample tap is not particularly limited, in order to make lid airtight live vessel sample tap, vessel can use common screw-in version or chimeric mode etc. with the juncture of lid, it is ensured that in container lysate do not run off with character constant.For security consideration, the material of lid uses the soft material such as polyethylene, polypropylene more suitable, does not recommend the adventurous sharp hard things such as glass.
Wherein, in cylinder, lysate is the saline solution of non-ionic octoxynol detergent, non-ionic octoxynol detergent can select to include NP40, or Chaps, or Tween20, or Triton X-100 etc., saline solution used can use the buffer agent between pH value 6-9, such as PBS(phosphate buffer solution), HEPES(citric acid solution), PIPES(sulfonic acid piperazine buffer solution), TES(ethyl sulfonic acid buffer solution), MOPS(propane sulfonic acid buffer solution) etc., it is also possible to add 0.9%NaCl(sodium chloride) normal saline.
Instrument is with an I-shaped double-faced piston, as it is shown in figure 1, feature is piston can not be pulled out completely cylindrical container body, piston ensure that the seal in pipe is outstanding, there is not the phenomenon of leakage.
Wherein, the material of I-shaped double-faced piston is the synthetic resin such as polyethylene, polypropylene, polrvinyl chloride, during the soft material such as particularly suitable polyethylene, polypropylene, because being prone to close and extraction, thus especially suitable.
Wherein, when seminal fluid translator does not uses, the connection post of double-faced piston is fully inserted in pipe, and its position is that outboard piston is affixed on outside cylindrical container body tail end baffle plate, as shown in Figure 2.Take essence and measure by outboard piston is pulled out, till inboard piston is affixed on inside cylindrical container body tail end baffle plate；And sample-adding amount is till pushing back outboard piston as cylindrical container body tail end baffle plate contact outside.Wherein, the volume of the sample volume of seminal fluid detection backward with seminal fluid cracking card sample-adding is equal, the ratio of this volume volume with the volume of sampling (or loading) by optimizing cylindrical container this cells in vivo lysate realizes, this volume can regulate according to design difference, as long as ensureing that the sample-adding amount after seminal fluid cracking adapts to the marginal value that detection card is corresponding.One of preferred version is 500-2000 microlitre for lysate volume in cylinder, measures seminal fluid and sample-adding amount volume is 50-200 microlitre.
Claims (10)
1. the quantitative seminal fluid translator simultaneously realizing gathering, crack, be loaded three steps, it is by the cylindrical container body with sample tap and tail end baffle plate, close the lid of sample tap, I-shaped double-faced piston through tail end baffle plate is constituted, translator is built with cell pyrolysis liquid, except the cell pyrolysis liquid with certain volume in cylindrical container body, also with the reserved air assisting mixing.
Quantitative seminal fluid translator the most according to claim 1, is characterized in that the sample tap that one end is prominent stack shell of cylindrical container body, and the other end of cylindrical container body is the baffle arrangement of hollow out, and middle aperture is connected post by I-shaped double-faced piston and runs through.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that the tail end baffle arrangement with hollow out, piston is blocked in work space by tail end baffle arrangement shape thicknesses, it is desirable to the aperture in the middle of it can accommodate the connection post of piston to be passed through, and stops piston to be extracted from cylindrical container body.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that with the lid closing sample tap, lid uses common screw-in version or chimeric mode with the juncture of cylindrical container body, it is ensured that the cell pyrolysis liquid within when lid does not takes off will not flow out or volatilize.
Quantitative seminal fluid translator the most according to claim 1, is characterized in that including sample tap, is designed to the taper of prominent vessel, with thin tube-like opening, it is ensured that when drawing seminal fluid and reverse mixing, lysate will not flow out；Simultaneously for enabling seminal fluid to be fully contacted with lysate, this tubular opening is less than 10 millimeters.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that with I-shaped double-faced piston, the I-shaped longitudinal section referring to this structure becomes the shape of " big centre, both sides is little ", the piston of its side is positioned at cylindrical container body interior, and piston area and shape are equal to the cross section, inner side of cylindrical container body, and are formed with the inwall of cylindrical container body and accommodate lysate and the closing space of air；The piston of I-shaped double-faced piston opposite side is positioned at outside cylindrical container body tip baffle plate, requirement ensure that it can not be pushed into cylindrical container body interior, facilitate push-and-pull during in order to use, be designed into major axis beyond cross section circle, ellipse, strip outside stack shell.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that I-shaped double-faced piston cannot be extracted from stack shell completely, the post that connects connecting two pistons in piston runs through the through aperture in the middle of cylindrical container body tip baffle plate, and is stablized by the realization that contacts with aperture.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that I-shaped double-faced piston both sides successively decide an inner carrier transportable length in cylinder with the moved distance that contacts of cylindrical container body tail end baffle plate, this length is equal to measure the volume of seminal fluid with the product of tangent plane in cylindrical container body, and this volume is also equal to liquid volume when sperm concentration is analyzed to detection card sample-adding.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that the length of cylindrical container body is longer than I-shaped double-faced piston and connects the length of post, except accommodating piston and connect the space of post, the headspace in cylindrical container body be enough to accommodate the spermatid lysate of certain volume and air.
Quantitative seminal fluid translator the most according to claim 1, it is characterized in that translator is built with cell pyrolysis liquid, the volume of this lysate measures volume with the seminal fluid determined by barrel diameter and I-shaped double-faced piston connecting rod and becomes fixed ratio, it is ensured that when I-shaped double-faced piston is released, the sample-adding amount of release adapts to the marginal value that detection card is corresponding.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620069329.8U CN205642913U (en) | 2016-01-25 | 2016-01-25 | Realize quantitative seminal fluid translator of collection, schizolysis, three step of application of sample simultaneously |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620069329.8U CN205642913U (en) | 2016-01-25 | 2016-01-25 | Realize quantitative seminal fluid translator of collection, schizolysis, three step of application of sample simultaneously |
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| CN205642913U true CN205642913U (en) | 2016-10-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108717111A (en) * | 2018-04-24 | 2018-10-30 | 金华市人民医院 | Full-automatic sperm detection device and method |
-
2016
- 2016-01-25 CN CN201620069329.8U patent/CN205642913U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108717111A (en) * | 2018-04-24 | 2018-10-30 | 金华市人民医院 | Full-automatic sperm detection device and method |
| CN108717111B (en) * | 2018-04-24 | 2023-11-10 | 金华市人民医院 | Full-automatic semen detection device and method |
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