CN205635610U - Little biological detection's preprocessing device - Google Patents
Little biological detection's preprocessing device Download PDFInfo
- Publication number
- CN205635610U CN205635610U CN201620436905.8U CN201620436905U CN205635610U CN 205635610 U CN205635610 U CN 205635610U CN 201620436905 U CN201620436905 U CN 201620436905U CN 205635610 U CN205635610 U CN 205635610U
- Authority
- CN
- China
- Prior art keywords
- pretreatment unit
- control device
- illumination system
- sample rack
- evenly mixing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a little biological detection's preprocessing device, preprocessing device utilizes reagent to implement the preprocessing device who handles to microbial sample, include: an adiactinic casing, a mixing device, set up in in the casing, the mixing device will reagent with microbial sample's mixture carries out the mixing, an illumination system, set up in in the casing, the illumination system with the mixing device sets up relatively, the illumination system is right the mixture carries out illumination. The utility model discloses a preprocessing device can realize the mixing function, also can carry out illumination and handle, still possesses the magazine function, and preprocessing device's mixing and illumination process operation step are simple and easy, save time, and mixing and illumination effect are fine, are favorable to follow -up little biological detection's accuracy.
Description
Technical field
This utility model relates to microorganism detection field, particularly relates to the pretreatment dress of a kind of microorganism detection
Put.
Background technology
At present, in food safety, ambient water quality detection and prevention and control of diseases work, the detection of microorganism
Technology mainly has conventional sense technology and molecular Biological Detection technology.And in molecular Biological Detection technology,
First micro-biological samples can be implemented pre-treatment or pretreatment, because in micro-biological samples both before microorganism detection
Comprising viable bacteria DNA and also comprise dead bacterium DNA, this type of micro-biological samples is through reagent nitrine ethidium bromide
(EMA) or nitrine bromination the third ingot (PMA) mixed exposure process after, described reagent can pass through dead bacterium cell
Film, and dead bacterium DNA strong bonded, thus suppress the polymerase chain reaction of follow-up dead bacterium DNA；And
Described reagent can not pass through living bacterial cells film, it is impossible to is combined with viable bacteria DNA, so that viable bacteria DNA is not
By described agents influence, next step polymerase chain reaction can be entered.In this way, can be
Viable bacteria DNA and dead bacterium region of DNA in micro-biological samples separate, to improve the standard of follow-up microorganism detection
Really property.
But, at present because being limited by the pretreatment unit of microorganism detection on current market so that micro-life
The preprocessing process very complicated of analyte detection, time and effort consuming, more especially for the granule containing total suspended solid
Complicated micro-biological samples, in its preprocessing process, reagent and micro-biological samples Blending Efficiency of Blending be not good enough；And it is pre-
In processing procedure during illumination, fraction solids granule and other impurity can be deposited to bottom, the light penetration power of exposure
Deficiency, uneven illumination one, it is unfavorable for the accuracy of follow-up microorganism detection.Therefore, prior art uses
The effectiveness comparison that the pretreatment unit of existing microorganism detection processes is poor, causes the standard of follow-up microorganism detection
Really property is the highest.
Utility model content
Technical problem to be solved in the utility model is the accuracy in order to improve follow-up microorganism detection,
In the preprocessing process of microorganism detection, the viable bacteria DNA in micro-biological samples and dead bacterium region of DNA are separated
Come.
For solving above-mentioned technical problem, the microorganism detection pretreatment unit that this utility model provides, described pre-
Processing means is the pretreatment unit utilizing reagent to process micro-biological samples enforcement, including:
One lighttight housing；
One evenly mixing device, is arranged in described housing, and described evenly mixing device is by described reagent and described microorganism
The mixture of sample mixes；And
One illumination system, is arranged in described housing, and described illumination system is oppositely arranged with described evenly mixing device,
Described illumination system carries out illumination to described mixture.
Further, described evenly mixing device is arranged at the bottom in described housing, and described illumination system is arranged at
Top in described housing.
Further, described evenly mixing device includes that a sample rack and controls device, and described sample rack is arranged at
On described control device, described control device controls the motion of described sample rack.
Further, described sample rack is provided with multiple sample slot for placing described mixture.
Optionally, described control device is a tumbler, and described control device drives described sample rack to rotate,
Described control device controls described sample rack and is mixed by described mixture in a rotative pattern.
Optionally, described control device is a vibrating device, and described control device drives the vibration of described sample rack,
Described control device controls described sample rack and is mixed by described mixture with mode of vibration.
Further, described illumination system includes that a light source, described light source are LED light source.
Preferably, described evenly mixing device and described illumination system are respectively by a single on-off control.
Compared with prior art, this utility model has the advantages that
Described pretreatment unit of the present utility model includes described evenly mixing device, described illumination system, therefore,
Described pretreatment unit can realize mixing function, also can carry out photo-irradiation treatment；And described evenly mixing device and
Described illumination system is respectively by a single on-off control, therefore, the mixing merit of described pretreatment unit
Both can separately can carry out with photo-irradiation treatment, it is also possible to operating, so, described pretreatment unit can be very simultaneously
The mixing of the good pretreatment realizing microorganism detection and lighting effect.It addition, described reagent is one sees light
Holding labile material, the housing of described pretreatment unit is lighttight, possesses the function of magazine, makes institute
State reagent and sun exposure around zero, the blending incubation effect of the most described pretreatment unit.
Finally, the mixing of described pretreatment unit and During Illumination operating procedure are simple, save the time, process
Effect is fine, can the viable bacteria DNA in micro-biological samples and dead bacterium region of DNA be separated completely, is conducive to
The accuracy of follow-up microorganism detection.
Accompanying drawing explanation
Fig. 1 is the structure top view of the pretreatment unit of microorganism detection in this utility model one embodiment.
Detailed description of the invention
Below in conjunction with flow chart and schematic diagram, the pretreatment unit of this utility model microorganism detection is carried out more
Detailed description, which show preferred embodiment of the present utility model, it should be appreciated that those skilled in the art
This utility model described here can be revised, and still realize advantageous effects of the present utility model.Therefore,
It is widely known that description below is appreciated that for those skilled in the art, and is not intended as this practicality
Novel restriction.
Referring to the drawings this utility model the most more particularly described below in the following passage.According to following theory
Bright and claims, advantage of the present utility model and feature will be apparent from.It should be noted that, accompanying drawing is all adopted
By the form simplified very much and all use non-ratio accurately, only in order to convenient, aid in illustrating this reality lucidly
By the purpose of new embodiment.
Core concept of the present utility model is, this utility model provides the pretreatment dress of a kind of microorganism detection
Putting, described pretreatment unit is the pretreatment unit utilizing reagent to process micro-biological samples enforcement, including:
One lighttight housing；One evenly mixing device, is arranged in described housing, and described evenly mixing device is by described reagent
Mix with the mixture of described micro-biological samples；And an illumination system, it is arranged in described housing,
Described illumination system is oppositely arranged with described evenly mixing device, and described illumination system carries out illumination to described mixture.
Described pretreatment unit can realize mixing function, also can carry out photo-irradiation treatment, and possesses the function of magazine,
Make described reagent and sun exposure around zero, can well realize the mixing of the pretreatment of microorganism detection
Hatch and lighting effect.
The mixing of described pretreatment unit and During Illumination operating procedure are simple, save the time, and treatment effect is very
Good, can completely the viable bacteria DNA in micro-biological samples and dead bacterium region of DNA be separated, follow-up micro-
The accuracy of biological detection.
It is exemplified below the embodiment of the pretreatment unit of described microorganism detection, to understand explanation this utility model
Content, it is understood that, content of the present utility model is not restricted to following example, and other pass through
The improvement of the routine techniques means of those of ordinary skill in the art is also within thought range of the present utility model.
As it is shown in figure 1, the pretreatment unit of the microorganism detection of the present embodiment includes a lighttight housing 10,
Described housing 10 has magazine function, and daylight can not be irradiated into, it is simple to follow-up blending incubation process.?
Being provided with illumination system 11 and an evenly mixing device 12 in described housing 10, described evenly mixing device 12 is with described
Illumination system 11 is oppositely arranged, it is also preferred that the left as it is shown in figure 1, described illumination system 11 is arranged at described shell
Top in body 10, described evenly mixing device 12 is arranged at the bottom in described housing 10, as described illumination
The vertical dimension of system 11 and described evenly mixing device 12 can be according to the size of described housing 10 or actual behaviour
Make and set；The light source of described illumination system 11 is a LED light source, as described evenly mixing device 12 includes
This 121 and one controls device 122, described sample rack 121 is provided with multiple sample for placing mixture
This groove 1211.
Preferably, in the present embodiment, described sample rack 121 arranges 6*4 totally 24 sample slot 1211,
And the quantity of the most described sample slot 1211 can set according to operational requirements, such as 6*3
Totally 18 sample slot etc..Described control device 122 is a tumbler, and described tumbler 122 includes one
Rotating shaft 1221 and one drives motor 1222, and described sample rack 121 is fixed in described rotating shaft 1221, by institute
Stating driving motor 1222 drives the described sample rack 121 in described rotating shaft 1221 to move in a rotative pattern, rotation
Turning direction can be clockwise, it is also possible to be counterclockwise.But, in other embodiments, institute
The control device 122 stating evenly mixing device 12 can also be a vibrating device, and described control device 122 drives institute
Stating sample rack 121 to vibrate, described control device 121 controls described sample rack 121 and realizes institute with mode of vibration
State the Blending Efficiency of Blending of mixture.
Preferably, in order to fully realize the During Illumination of the preprocess method of microorganism detection, described LED light
Source is made up of the 24 LEDs lamp bead arranged by 6*4 mode, and the direction of illumination of described illumination system 11 is vertical
Described rotating shaft 1221.Knowable, described LED light source is to act on the mixture in described sample rack 121,
Therefore, the LED lamp bead quantity of described LED light source and arrangement do not limit, as long as meet can realize described pre-
During Illumination in processing method.
Concrete, in order to be able to be implemented separately the effect of mixing and illumination, described illumination system 11 and described mixing
Device 12 works independently from each other, and both are respectively by a single on-off control.
Pretreatment unit described in the present embodiment includes described evenly mixing device 12, described illumination system 11, because of
This, described pretreatment unit can realize mixing function, also can carry out photo-irradiation treatment；And described mixing dress
Put 12 and described illumination system 11 be respectively by a single on-off control, therefore, described pre-place
Mixing function and the photo-irradiation treatment of reason device both can separately be carried out, it is also possible to operate simultaneously, so, described
Pretreatment unit can well realize mixing and the lighting effect of the pretreatment of different types of microorganism detection.
Seeing that light holds labile material it addition, described reagent is one, the housing 10 of described pretreatment unit is impermeable
Light, possess the function of magazine, make described reagent and sun exposure around zero, the most described pre-place
The blending incubation effect of reason device.
Mixing and During Illumination to pretreatment unit described in the present embodiment are described further below, in order to
Content of the present utility model is more clearly described.
First, prepare mixture, the reagent of suitable concn is added in micro-biological samples, forms described mixing
Thing.Described reagent is nitrine ethidium bromide or nitrine bromination the third ingot.
Then, carry out blending incubation, multiple described mixture are respectively placed in described pretreatment unit
In described sample slot 1211, and laid and fixed；Then, open described evenly mixing device 12, described in drive
Dynamic motor 1222 drives described sample rack 121 to move in a rotative pattern, the speed of rotation control 1-100 turn/
Point, can regulate and control for different types of micro-biological samples, rotational time is 5min-15min, actual behaviour
In work, can be adjusted for different types of micro-biological samples, make the abundant blending incubation of described mixture.
Finally, carry out illumination, open described illumination system 11, the described mixture of blending incubation is carried out light
According to, irradiation time is 10min-20min.In fact, above-mentioned carry out illumination during, described mixed in order to make
Compound (sample that particularly granule Han total suspended solid is more) is always maintained at the state of mixing, can be simultaneously
Open described evenly mixing device 12, it is achieved mix the process operation of illumination.So, regardless of microorganism
Sample is simple or complicated, can viable bacteria DNA therein and dead bacterium region of DNA be separated completely.
The mixing of pretreatment unit described in the present embodiment and During Illumination operating procedure are simple, save the time,
And mix with lighting effect meeting more preferably.
To sum up, the pretreatment unit of microorganism detection described in the utility model is realized by described evenly mixing device
Mixing function, carries out photo-irradiation treatment by described illumination system；And described evenly mixing device and described illumination system
System is respectively by a single on-off control, therefore, at the mixing function of described pretreatment unit and illumination
Reason both can separately be carried out, it is also possible to operating, so, described pretreatment unit can well realize not simultaneously
The mixing of pretreatment of microorganism detection and lighting effect with type.It addition, described reagent is one sees light
Holding labile material, the housing of described pretreatment unit is lighttight, possesses the function of magazine, makes institute
State reagent and sun exposure around zero, the blending incubation effect of the most described pretreatment unit.
Meanwhile, the mixing of described pretreatment unit of the present utility model and During Illumination operating procedure are simple, joint
Making an appointment, treatment effect is fine, can the viable bacteria DNA in micro-biological samples and dead bacterium region of DNA be divided completely
Come, the accuracy of follow-up microorganism detection.
Obviously, those skilled in the art this utility model can be carried out various change and modification without deviating from
Spirit and scope of the present utility model.So, if these amendments of the present utility model and modification belong to this reality
Within the scope of novel claim and equivalent technologies thereof, then this utility model is also intended to comprise these changes
With including modification.
Claims (8)
1. the pretreatment unit of a microorganism detection, it is characterised in that described pretreatment unit is to utilize examination
The pretreatment unit that micro-biological samples enforcement is processed by agent, including:
One lighttight housing；
One evenly mixing device, is arranged in described housing, and described evenly mixing device is by described reagent and described micro-life
The mixture of thing sample mixes；And
One illumination system, is arranged in described housing, and described illumination system is relative with described evenly mixing device to be set
Putting, described illumination system carries out illumination to described mixture.
2. pretreatment unit as claimed in claim 1, it is characterised in that described evenly mixing device is arranged at institute
Stating the bottom in housing, described illumination system is arranged at the top in described housing.
3. pretreatment unit as claimed in claim 1, it is characterised in that as described evenly mixing device includes
This and one controls device, and described sample rack is arranged on described control device, and described control device controls institute
State the motion of sample rack.
4. pretreatment unit as claimed in claim 3, it is characterised in that be provided with many on described sample rack
The individual sample slot for placing described mixture.
5. pretreatment unit as claimed in claim 3, it is characterised in that described control device is a rotation
Device, described control device drives described sample rack to rotate, and described control device controls described sample rack with rotation
Described mixture is mixed by the mode that turns.
6. pretreatment unit as claimed in claim 3, it is characterised in that described control device is a vibration
Device, described control device drives the vibration of described sample rack, and described control device controls described sample rack to shake
Described mixture is mixed by flowing mode.
7. pretreatment unit as claimed in claim 1, it is characterised in that described illumination system includes a light
Source, described light source is LED light source.
8. pretreatment unit as claimed in claim 1, it is characterised in that described evenly mixing device and described light
Lighting system is respectively by a single on-off control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620436905.8U CN205635610U (en) | 2016-05-13 | 2016-05-13 | Little biological detection's preprocessing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620436905.8U CN205635610U (en) | 2016-05-13 | 2016-05-13 | Little biological detection's preprocessing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205635610U true CN205635610U (en) | 2016-10-12 |
Family
ID=57058346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620436905.8U Active CN205635610U (en) | 2016-05-13 | 2016-05-13 | Little biological detection's preprocessing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205635610U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760212A (en) * | 2021-01-21 | 2021-05-07 | 上海海洋大学 | Fast live bacteria DNA screening instrument, and use method and application thereof |
| CN113403192A (en) * | 2021-06-16 | 2021-09-17 | 惠州市阳光生物科技有限公司 | Microorganism detection device, microorganism detection system, and microorganism detection method |
-
2016
- 2016-05-13 CN CN201620436905.8U patent/CN205635610U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760212A (en) * | 2021-01-21 | 2021-05-07 | 上海海洋大学 | Fast live bacteria DNA screening instrument, and use method and application thereof |
| CN113403192A (en) * | 2021-06-16 | 2021-09-17 | 惠州市阳光生物科技有限公司 | Microorganism detection device, microorganism detection system, and microorganism detection method |
| CN113403192B (en) * | 2021-06-16 | 2024-02-27 | 广东阳光生物科技有限公司 | Microorganism detection device, microorganism detection system, and microorganism detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205635610U (en) | Little biological detection's preprocessing device | |
| CN104743677B (en) | A kind of method utilizing Immobilized photosynthetic bacteria membrane reaction coupling system to process waste water | |
| EP1614465B8 (en) | Microfluidic system for chemical analysis | |
| CN105424629A (en) | Micro-fluidic chip and copper ion detection system | |
| CN105062888A (en) | Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument | |
| Pantazi et al. | The smart aquaponics greenhouse–an interdisciplinary educational laboratory | |
| Li et al. | Fluorescence analysis of antibiotics and antibiotic-resistance genes in the environment: A mini review | |
| Agustina et al. | Reduction of copper, iron, and lead content in laboratory wastewater using zinc oxide photocatalyst under solar irradiation | |
| CN205666968U (en) | Heating device for biochemistry | |
| CN205794518U (en) | A kind of ecological balanced system of the aquatic animal density culture acting on oxygen consumption | |
| CN110935364A (en) | Chemical reagent mixing arrangement | |
| CN205672860U (en) | A kind of biochemistry stock stirring device | |
| Mohammed et al. | Optimisation of red light-emitting diodes irradiance for illuminating mixed microalgal culture to treat municipal wastewater | |
| CN105289670B (en) | A kind of SiO of single-minded identification2/Ag3PO4@ZnFe2O4Inorganic trace composite photo-catalyst and preparation method thereof | |
| CN101182066B (en) | Photo-biological reactor for treatment of high-chroma waste water | |
| Eitzmann et al. | Versatile dual-channel loop-mediated isothermal amplification assay featuring smartphone imaging enables determination of fecal indicator bacteria in environmental waters | |
| CN205517429U (en) | A machine for producing lung formula aeration sponge | |
| CN207646198U (en) | A kind of luminous agitating paddle for photosynthesis physiological target | |
| CN205794520U (en) | A kind of special equipment for cultivating water oxygen supplement virus killing integration | |
| CN109574207A (en) | A kind of algal gel cogeneration system for textile printing and dyeing wastewater processing | |
| JP2010233551A (en) | Method for culturing microalga | |
| CN205192941U (en) | Micro -fluidic chip and copper ion detecting system | |
| Liu et al. | Design and application of a solar mobile pond aquaculture water quality-regulation machine based in Bream Pond aquaculture | |
| CN209583686U (en) | A kind of magnetic adsorbent reaction generating device | |
| CN204202970U (en) | A kind of liquid blending device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |