CN205635573U - Horizontal inside and outside dual cycle airlift bioreactor - Google Patents
Horizontal inside and outside dual cycle airlift bioreactor Download PDFInfo
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- 239000007921 spray Substances 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 abstract description 18
- 239000001301 oxygen Substances 0.000 abstract description 18
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- 230000007423 decrease Effects 0.000 abstract description 2
- 238000009434 installation Methods 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 description 18
- 230000004151 fermentation Effects 0.000 description 18
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- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
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- 238000011031 large-scale manufacturing process Methods 0.000 description 1
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Abstract
本实用新型公开了一种卧式内外双循环气升式生物反应器，卧式反应釜体的左右侧对称的安装外循环上升管，外循环上升管的上下端分别与卧式反应釜体的上下部连通，外循环上升管下部安装有空气喷嘴，并在外循环上升管管壁外周安装外置换热器，所述的内循环导流筒设置于卧式反应釜体内部并与卧式反应釜体中心同轴安装，在内循环导流筒上设有多个筛孔，所述的空气分布器设置于卧式反应釜体内部并位于内循环导流筒正下方，与卧式反应釜体同轴安装，空气分布器的下端穿出卧式反应釜体的底面与空气进口连通，所述的内置换热器设置在卧式反应釜体的内壁上。本实用新型从根本上解决了常规气升式反应器内高静压下，溶氧率降低的问题。
The utility model discloses a horizontal internal and external double circulation air-lift bioreactor. The left and right sides of the horizontal reactor body are symmetrically installed with external circulation ascending pipes. The upper and lower parts are connected, and the lower part of the outer circulation riser is equipped with an air nozzle, and an external heat exchanger is installed on the outer circumference of the outer circulation riser pipe wall. The center of the body is installed coaxially, and a plurality of screen holes are arranged on the inner circulation guide tube. Coaxial installation, the lower end of the air distributor passes through the bottom surface of the horizontal reactor body and communicates with the air inlet, and the built-in heat exchanger is arranged on the inner wall of the horizontal reactor body. The utility model fundamentally solves the problem that the dissolved oxygen rate decreases under the high static pressure in the conventional air-lift reactor.
Description
技术领域 technical field
本实用新型涉及微生物发酵设备技术领域，尤其涉及一种卧式内外双循环气升式生物反应器。 The utility model relates to the technical field of microbial fermentation equipment, in particular to a horizontal air-lift bioreactor with internal and external double circulation.
背景技术 Background technique
大多数的微生物发酵都是好氧的，然而氧气在发酵醪液中的溶解度极小，仅0.22mmol/L，是最容易成为微生物发酵的限制性因素，大大影响了生化反应的进行。 Most microbial fermentations are aerobic. However, the solubility of oxygen in the fermentation mash is extremely small, only 0.22mmol/L, which is the most likely limiting factor for microbial fermentation and greatly affects the progress of biochemical reactions.
气升式反应器是好氧反应器的一种，利用通入反应器内的空气上升时的动力带动作用及发酵液密度变化，实现醪液混合；结构分为内循环和外循环反应器两种形式，其特点包括：结构简单、能耗低、节省钢材、操作无噪音、装液系数高、不须加消泡剂、维修操作清洗简单、减少杂菌感染等特点。气升式发酵罐符合工艺要求及经济指标应至少包括两点：一是循环周期时间必须符合菌种发酵的需要；二是要选用适当直径的喷嘴，保证氧气的供给。 The air-lift reactor is a kind of aerobic reactor, which realizes the mixing of the mash by using the power of the air passing into the reactor to rise and the change of the density of the fermentation liquid; the structure is divided into two types: internal circulation and external circulation Its characteristics include: simple structure, low energy consumption, steel saving, noiseless operation, high liquid filling coefficient, no need to add defoamer, simple maintenance and operation cleaning, and reduced bacterial infection. The air-lift fermenter meets the process requirements and economic indicators should include at least two points: one is that the cycle time must meet the needs of bacterial fermentation; the other is to choose a nozzle with an appropriate diameter to ensure the supply of oxygen.
然而，现有的气升式反应器在高浓度、高粘度醪液培养时循环周期时间上不达标，导致醪液混合不均匀、传质效果差；单纯选用适当直径的喷嘴也满足不了高浓度、高粘度的醪液的对氧气的需求，导致溶氧不充分，发酵周期长。另外，工业上用于大生产的气升式反应器往往高达几十米，静压对溶氧量影响严重。本实用新型从改善高浓度、高粘度的大型生物反应设备的气液混匀效果出发，设计一种高溶氧、高传质、剪切力小的生物反应器。 However, the existing airlift reactors do not meet the standard in terms of cycle time when cultivating high-concentration and high-viscosity mash, resulting in uneven mixing of mash and poor mass transfer effect; simple selection of nozzles with appropriate diameters cannot meet the requirements of high-concentration and high-viscosity mash 1. The demand for oxygen of the high-viscosity mash leads to insufficient dissolved oxygen and a long fermentation cycle. In addition, the air-lift reactors used for large-scale production in industry are often tens of meters high, and the static pressure has a serious impact on the amount of dissolved oxygen. The utility model designs a bioreactor with high dissolved oxygen, high mass transfer and low shear force, starting from improving the gas-liquid mixing effect of large-scale bioreactors with high concentration and high viscosity.
实用新型内容 Utility model content
本实用新型目的就是为了弥补已有技术的缺陷，提供一种卧式内外双循环气升式生物反应器。 The purpose of the utility model is to provide a horizontal air-lift bioreactor with internal and external double circulation in order to make up for the defects of the prior art.
本实用新型是通过以下技术方案实现的： The utility model is achieved through the following technical solutions:
一种卧式内外双循环气升式生物反应器，包括卧式反应釜体、外循环上升管、外置换热器、空气喷嘴、空气分布器、内置换热器和内循环导流筒，卧式反应釜体的左右侧对称的安装外循环上升管，外循环上升管的上下端分别与卧式反应釜体的上下部连通，外循环上升管下部安装有空气喷嘴，并在外循环上升管管壁外周安装外置换热器，所述的内循环导流筒设置于卧式反应釜体内部并与卧式反应釜体中心同轴安装，在内循环导流筒上设有多个筛孔，所述的空气分布器设置于卧式反应釜体内部并位于内循环导流筒正下方，与卧式反应釜体同轴安装，空气分布器的下端穿出卧式反应釜体的底面与空气进口连通，所述的内置换热器设置在卧式反应釜体的内壁上，所述的空气进口置于卧式反应釜体的底部，在卧式反应釜体的底部还设有出料口，在卧式反应釜体的上部设有进料口和排气口。内循环导流筒和外循环上升管二者共同作用，借助空气的推动力及密度的变化，发酵醪液在反应釜内形成两大循环，使醪液混合均匀，实现高溶氧、高传质。 A horizontal internal and external double-circulation airlift bioreactor, including a horizontal reactor body, an external circulation riser, an external heat exchanger, an air nozzle, an air distributor, a built-in heat exchanger and an internal circulation guide tube, the horizontal The left and right sides of the vertical reactor body are symmetrically installed with external circulation rising pipes. The upper and lower ends of the external circulation rising pipes are respectively connected with the upper and lower parts of the horizontal reactor body. An external heat exchanger is installed on the outer periphery of the wall, and the internal circulation guide tube is arranged inside the horizontal reactor body and coaxially installed with the center of the horizontal reactor body, and a plurality of screen holes are arranged on the internal circulation guide tube. The air distributor is arranged inside the horizontal reactor body and directly below the internal circulation guide tube, and is installed coaxially with the horizontal reactor body, and the lower end of the air distributor passes through the bottom surface of the horizontal reactor body and the air The inlet is connected, the built-in heat exchanger is arranged on the inner wall of the horizontal reactor body, the air inlet is placed at the bottom of the horizontal reactor body, and a discharge port is also provided at the bottom of the horizontal reactor body , There is a feed port and an exhaust port on the upper part of the horizontal reactor body. The internal circulation guide tube and the external circulation riser work together, and with the help of the driving force of the air and the change of density, the fermented mash forms two major circulations in the reactor, so that the mash is evenly mixed to achieve high dissolved oxygen and high water transfer. quality.
所述的内循环导流筒的每个筛孔的直径在2-10mm之间，并由下向上逐渐变大，筛孔的总面积占内循环导流筒面积的20%-60%。筛孔的总面积占导流筒面积的20%~60%，筛孔的直径及总面积随发酵微生物的不同可调整。该设计使得导流筒中的部分初始气泡能瞬间传递到釜中，增大溶氧。 The diameter of each sieve of the inner circulation guide cylinder is between 2-10mm, and gradually increases from bottom to top, and the total area of the sieve holes accounts for 20%-60% of the area of the inner circulation guide cylinder. The total area of the sieve hole accounts for 20%~60% of the area of the guide cylinder, and the diameter and total area of the sieve hole can be adjusted according to the different fermentation microorganisms. This design allows part of the initial bubbles in the draft tube to be instantly transferred to the kettle to increase dissolved oxygen.
所述的空气分布器包括有水平密封圆盘，所述水平密封圆盘连通空气进口，水平密封圆盘表面均匀设置多个空气分布孔。在不同的发酵体系以及不同发酵时期中，细胞浓度及需氧量不同，对循环周期的要求也不同；通过调整空气进口的速率，来调控循环周期，满足微生物对高溶氧的需求。 The air distributor includes a horizontal sealing disc, which communicates with the air inlet, and a plurality of air distribution holes are uniformly arranged on the surface of the horizontal sealing disc. In different fermentation systems and different fermentation periods, the cell concentration and oxygen demand are different, and the requirements for the cycle period are also different; by adjusting the rate of air inlet, the cycle cycle can be adjusted to meet the needs of microorganisms for high dissolved oxygen.
所述的卧式反应釜体为圆筒形，罐体的径高比为1.5-3。 The horizontal reactor body is cylindrical, and the diameter-to-height ratio of the tank body is 1.5-3.
所述的空气喷嘴以100～500m/s的速度喷入上升管。 The air nozzle sprays into the riser at a speed of 100-500m/s.
所述的内置换热器置于卧式反应釜体内部两侧或一侧，起到调节反应温度的作用，并作为挡板，进一步混匀发酵醪液。 The built-in heat exchangers are placed on both sides or one side of the horizontal reactor body to adjust the reaction temperature and serve as baffles to further mix the fermented mash.
由以上技术方案可以看出，本实用新型通过卧式内外双循环气升式生物反应器，采用内外双循环装置及空气分布器供氧，包括带有空气喷嘴的外循环上升管、带有不同直径及总面积比的内循环导流筒、特殊的带有多个空气分布孔的圆盘式空气分布器及外置、内置换热器。不论发酵醪液浓度、粘度的高低，不论天气炎热与否，该新型反应器均能使醪液传质、传热、溶氧效率高，可缩短发酵周期。 It can be seen from the above technical scheme that the utility model uses a horizontal internal and external double circulation air-lift bioreactor, adopts an internal and external double circulation device and an air distributor to supply oxygen, including an external circulation riser with an air nozzle, with different The diameter and the total area ratio of the internal circulation guide tube, a special disc-type air distributor with multiple air distribution holes and external and internal heat exchangers. Regardless of the concentration and viscosity of the fermented mash, and regardless of whether the weather is hot or not, the new reactor can make the mash mass transfer, heat transfer, and oxygen-dissolving efficiency high, and can shorten the fermentation cycle.
本实用新型的优点是：本实用新型从根本上解决了常规气升式反应器内高静压下，溶氧率降低的问题，同时，由于外循环上升管中空气喷嘴的安装、内循环导流筒上不均匀筛孔的设计，及带均匀分布孔的圆盘式空气分布器的设计，使得釜体内各个空间的溶氧率迅速增大，满足发酵需要。本实用新型卧式双循环气升式生物反应器从根本上解决了常规气升式反应器不适合高浓度、高粘度的发酵体系；本实用新型采用外循环上升管及内循环导流筒的内外双循环装置，使得发酵醪液在釜体内外形成内、外两大循环，增强反应器的混合效果；并且由于上升管较短，高浓度、高粘度的发酵醪液借助位于上升管下部的空气喷嘴的推动作用，即可快速地通过，阻力较小。本实用新型传质效果良好，特别适合高浓度、高粘度的发酵体系。本实用新型卧式双循环气升式生物反应器采用外置、内置双换热器，即强化了管内外热量传递，内置换热器又可作为挡板，增强醪液传质；本实用新型卧式内外双循环气升式生物反应器卧置，占地少、不借助搅拌装置而实现高浓度、高粘度发酵体系、能耗小、大大节省了成本；另外，本实用新型结构简单，检修方便，可操作性强。 The utility model has the advantages that: the utility model fundamentally solves the problem that the dissolved oxygen rate decreases under the high static pressure in the conventional air-lift reactor; The design of uneven sieve holes on the flow cylinder and the design of the disc-type air distributor with uniformly distributed holes make the dissolved oxygen rate in each space in the kettle body increase rapidly to meet the needs of fermentation. The utility model's horizontal double-circulation air-lift bioreactor fundamentally solves the problem that conventional air-lift reactors are not suitable for high-concentration and high-viscosity fermentation systems; The internal and external double circulation device makes the fermented mash form two major circulations inside and outside the kettle body, which enhances the mixing effect of the reactor; and because the rising pipe is short, the fermented mash with high concentration and high viscosity is located at the lower part of the rising pipe. The push action of the air nozzle can pass through quickly with less resistance. The utility model has good mass transfer effect, and is especially suitable for fermentation systems with high concentration and high viscosity. The utility model's horizontal double-cycle air-lift bioreactor adopts external and built-in double heat exchangers, which strengthens the heat transfer inside and outside the tube, and the built-in heat exchanger can also be used as a baffle to enhance the mass transfer of mash; the utility model Horizontal internal and external double-circulation air-lift bioreactor is placed horizontally, occupies less land, realizes high-concentration and high-viscosity fermentation system without the aid of a stirring device, consumes less energy, and greatly saves costs; in addition, the utility model has a simple structure and is easy to maintain Convenient and operable.
附图说明 Description of drawings
图1为本实用新型的结构示意图。 Fig. 1 is the structural representation of the utility model.
图2为本实用新型内循环导流筒结构图。 Fig. 2 is a structural diagram of the inner circulation guide tube of the utility model.
具体实施方式 detailed description
如图1、2所示，一种卧式内外双循环气升式生物反应器，包括卧式反应釜体1、外循环上升管2、外置换热器3、空气喷嘴4、空气分布器6、内置换热器7和内循环导流筒5，卧式反应釜体1的左右侧对称的安装外循环上升管2，外循环上升管2的上下端分别与卧式反应釜体1的上下部连通，外循环上升管2下部安装有空气喷嘴4，并在外循环上升管2管壁外周安装外置换热器3，所述的内循环导流筒5设置于卧式反应釜体1内部并与卧式反应釜体1中心同轴安装，在内循环导流筒5上设有多个筛孔12，所述的空气分布器6设置于卧式反应釜体1内部并位于内循环导流筒5正下方，与卧式反应釜体1同轴安装，空气分布器6的下端穿出卧式反应釜体1的底面与空气进口10连通，所述的内置换热器7设置在卧式反应釜体1的内壁上，所述的空气进口10置于卧式反应釜体1的底部，在卧式反应釜体1的底部还设有出料口9，在卧式反应釜体1的上部设有进料口8和排气口11。内循环导流筒5和外循环上升管2二者共同作用，借助空气的推动力及密度的变化，发酵醪液在反应釜内形成两大循环，使醪液混合均匀，实现高溶氧、高传质。 As shown in Figures 1 and 2, a horizontal internal and external double-circulation airlift bioreactor includes a horizontal reactor body 1, an external circulation riser 2, an external heat exchanger 3, an air nozzle 4, and an air distributor 6 , a built-in heat exchanger 7 and an internal circulation guide tube 5, the left and right sides of the horizontal reactor body 1 are symmetrically installed with an outer circulation riser 2, and the upper and lower ends of the outer circulation riser 2 are respectively connected to the upper and lower sides of the horizontal reactor body 1. The lower part of the outer circulation riser 2 is equipped with an air nozzle 4, and an external heat exchanger 3 is installed on the outer circumference of the wall of the outer circulation riser 2, and the inner circulation guide tube 5 is arranged inside the horizontal reactor body 1 and Installed coaxially with the center of the horizontal reactor body 1, a plurality of sieve holes 12 are provided on the internal circulation guide cylinder 5, and the air distributor 6 is arranged inside the horizontal reactor body 1 and located in the internal circulation guide Directly below the cylinder 5, it is coaxially installed with the horizontal reactor body 1, and the lower end of the air distributor 6 passes through the bottom surface of the horizontal reactor body 1 to communicate with the air inlet 10, and the built-in heat exchanger 7 is arranged in the horizontal reactor body 1 On the inner wall of the reactor body 1, the air inlet 10 is placed at the bottom of the horizontal reactor body 1, and a discharge port 9 is also provided at the bottom of the horizontal reactor body 1, and at the bottom of the horizontal reactor body 1 The upper part is provided with a feed port 8 and an exhaust port 11 . The inner circulation guide tube 5 and the outer circulation ascending pipe 2 work together, and with the help of the driving force of the air and the change of density, the fermented mash forms two major circulations in the reactor, so that the mash is mixed evenly to achieve high dissolved oxygen, High mass transfer.
所述的内循环导流筒5的每个筛孔的直径在2-10mm之间，并由下向上逐渐变大，筛孔的总面积占内循环导流筒面积的20%-60%。筛孔的总面积占导流筒面积的20%~60%，筛孔的直径及总面积随发酵微生物的不同可调整。该设计使得导流筒中的部分初始起泡能瞬间传递到釜中，增大溶氧。 The diameter of each sieve hole of the internal circulation guide cylinder 5 is between 2-10mm, and gradually increases from bottom to top, and the total area of the sieve holes accounts for 20%-60% of the area of the internal circulation guide cylinder. The total area of the sieve hole accounts for 20%~60% of the area of the guide cylinder, and the diameter and total area of the sieve hole can be adjusted according to the different fermentation microorganisms. This design allows part of the initial foaming in the draft tube to be instantly transferred to the kettle, increasing the dissolved oxygen.
所述的空气分布器6包括有水平密封圆盘，所述水平密封圆盘连通空气进口，水平密封圆盘表面均匀设置多个空气分布孔。在不同的发酵体系以及不同发酵时期中，细胞浓度及需氧量不同，对循环周期的要求也不同；通过调整空气进口的速率，来调控循环周期，满足微生物对高溶氧的需求。 The air distributor 6 includes a horizontal sealing disc, which communicates with the air inlet, and a plurality of air distribution holes are uniformly arranged on the surface of the horizontal sealing disc. In different fermentation systems and different fermentation periods, the cell concentration and oxygen demand are different, and the requirements for the cycle period are also different; by adjusting the rate of air inlet, the cycle cycle can be adjusted to meet the needs of microorganisms for high dissolved oxygen.
所述的卧式反应釜体1为圆筒形，罐体的径高比为1.5-3。 The horizontal reactor body 1 is cylindrical, and the diameter-to-height ratio of the tank body is 1.5-3.
所述的空气喷嘴4以100～500m/s的速度喷入上升管。 The air nozzle 4 sprays into the riser at a speed of 100-500m/s.
所述的外置换热器7置于卧式反应釜体内部两侧或一侧，起到调节反应温度的作用，并作为挡板，进一步混匀发酵醪液。 The external heat exchanger 7 is placed on both sides or one side of the horizontal reactor body to regulate the reaction temperature and serve as a baffle to further mix the fermented mash.
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| CN201620365499.0U Expired - Fee Related CN205635573U (en) | 2016-04-26 | 2016-04-26 | Horizontal inside and outside dual cycle airlift bioreactor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779273A (en) * | 2016-04-26 | 2016-07-20 | 淮南师范学院 | Horizontal internal and external double-circulation airlift bioreactor |
| CN111635861A (en) * | 2020-06-30 | 2020-09-08 | 江南大学 | An airlift reactor for animal cell culture |
| CN112844280A (en) * | 2020-12-23 | 2021-05-28 | 中国纺织科学研究院有限公司 | Horizontal reaction kettle, functional polyester production system applying same and production method |
-
2016
- 2016-04-26 CN CN201620365499.0U patent/CN205635573U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779273A (en) * | 2016-04-26 | 2016-07-20 | 淮南师范学院 | Horizontal internal and external double-circulation airlift bioreactor |
| CN111635861A (en) * | 2020-06-30 | 2020-09-08 | 江南大学 | An airlift reactor for animal cell culture |
| CN112844280A (en) * | 2020-12-23 | 2021-05-28 | 中国纺织科学研究院有限公司 | Horizontal reaction kettle, functional polyester production system applying same and production method |
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