CN205570357U - Device of biological sample is drawed and detected - Google Patents
Device of biological sample is drawed and detected Download PDFInfo
- Publication number
- CN205570357U CN205570357U CN201620006790.9U CN201620006790U CN205570357U CN 205570357 U CN205570357 U CN 205570357U CN 201620006790 U CN201620006790 U CN 201620006790U CN 205570357 U CN205570357 U CN 205570357U
- Authority
- CN
- China
- Prior art keywords
- mixing chamber
- sample
- storage container
- processing unit
- magnetic bead
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses a device of biological sample is drawed and detected, specifically, the utility model discloses an it is totally closed, device and relevant equipment easy operation, carry out purification, transfer, collection and measuring to biological sample. The device has the magnet of removal, magnetic field shielding and open design, can carry out magnetic bead liquid mixing, magnetic separation, magnetic bead transfer at totally closed pipeline. Use the device accessible is manual, semi -automatic, full -automatic operation carries out quickly separating purification, transfer, collection and measuring to biological sample median nucleic acid / albumen / micro molecule / cell.
Description
Technical field
This utility model relates to biological specimen preparation and detection field, this utility model provides a kind of totally-enclosed, liquid feeding, solid-liquid mixing, Magneto separate, sample transfer and the device detected and instrument can be carried out, biological specimen can be carried out efficient purification, shift, detect or preserve.
Background technology
The sample of biological detection can be blood, saliva, oral mucosa, body fluid, hair root, feces, tissue etc., and these biological specimens carry out sample separation and purification treatment, it is thus achieved that nucleic acid, albumen, little molecule, cell be in medical test must be through step.The blood or the other biological sample that gather would generally deliver to carry out in laboratory separation and Extraction, owing to these biological specimens are potential virus and the antibacterial source of infection, process these biological samples in open systems and can bring threat to operator's life and health, in addition, owing to some purification and detectable are likely to result in harmful effect to the health of laboratory environment and operator, a kind of totally enclosed biological sample purification and detection device and instrument can be prevented effectively from operator and be constantly exposed to the source of infection and harmful chemical agents, reduce cross-contamination between sample simultaneously, improve detection accuracy.
Magnetic microsphere can be with active adsorption and purifying biological molecule, including nucleic acid, protein, little molecule, cell.Biomolecule can be attached on magnetic-particle by magnetic microsphere surface functionalities group, carries out washing purification by magnetic field, and impurity is washed away by selectivity, thus reaches the effect of the specific biomolecule of purification.Magnetic microsphere has been widely used for biological specimen and separates and detection, nucleic acid extraction, immune detection etc., and is suitable for totally closed operation.
Accordingly, it would be desirable to a kind of totally-enclosed, manually or automatically instrument can be used to carry out liquid feeding, solid-liquid mixing, Magneto separate, the device of sample transfer and instrument and biological specimen to be purified, shift, detect or preserves.This small-sized, device simple to operate and instrument can be the bringing great convenience property such as sample collection, molecule experiments, clinical diagnosis.
Utility model content
The purpose of this utility model is to provide one and can manually carry out totally-enclosed, and the device being purified biological specimen, shift, detect or preserving simple to operate, this equipment is used as instrument and is purified detection process.
First aspect of the present utility model, it is provided that a kind of biological specimen purification, collect and detect device, described device includes:
(a) multiple processing unit, the most each processing unit includes:
(a1) one or more storage containers, described storage container can store liquid, solid or solid-liquid suspended matter；
(a2) and the adjacent mixing chamber of described storage container, described mixing chamber is used for making magnetic bead contact with liquid；(preferably passing through to rotate, tilt or shake to make magnetic bead and liquid be fully contacted)；
(a3) locking device between described storage container and mixing chamber, described locking device can make to be between storage container and the mixing chamber of described processing unit connection or not connected state；(preferably by switch, can puncture seal film control connection or close)；Preferably, there is also filter membrane between described storage container and mixing chamber；
B (), between adjacent mixing chambers, for connecting the connecting tube of adjacent processing units, described connecting tube has pipeline connecting valve, described pipeline connecting valve can regulate and make described connecting tube be in the state of being turned on and off；Preferably, there is also filter membrane between described adjacent connecting tube；
(c) field generating unit, described field generating unit is positioned near described multiple mixing chambers (such as lower section, side etc.), and single described mixing chamber can be made to be in magnetic field environment at special time period, make remaining described mixing chamber be in non-magnetic field environment simultaneously, or make single described mixing chamber be in strong magnetic field circumstance, and other mixing chamber is made to be in more weak magnetic field environment (the most single removable permanent magnet)；
With optional (d) sample collection tube and/or sensing chamber.
In another preference, control the magnetic bead movement at different mixing chambers by the location of field generating unit.
In another preference, controlled by the angle of inclination of pipeline, connecting tube be turned on and off and field generating unit location makes magnetic bead separate with liquid and to be in two adjacent different mixing indoor.
In another preference, described field generating unit body Han magnetic field shielding, it is positioned near mixing chamber (such as lower section, side etc.), and the action of a magnetic field that mixing chamber is produced by field generating unit can be blocked, make described mixing chamber be in non-magnetic field environment.
In another preference, described storage device is mixing chamber.
In another preference, described device is totally enclosed, and in use, described device is internal does not occur material to exchange outside device to wherein said totally-enclosed finger.
In another preference, described storage container has valve switch, and described storage container can be made to be in opening or closed mode by the valve switch described in regulation.
In another preference, described storage container have valve switch and can puncture seal film, and can by regulation described in valve switch auxiliary device puncture seal film make described storage container be in opening or closed mode.
In another preference, described storage container has removable opening, and described storage container can be made to be in opening or closed mode by the removable opening described in regulation.
In another preference, the described container that stores has a sample-adding portion can thrust or penetrate.
In another preference, the described container that stores is the plastic containers being pre-loaded with quantitative liquid/solid/solid-liquid suspended matter.
In another preference, the described container that stores is the plastic containers with sting device being pre-loaded with quantitative liquid/solid/solid-liquid suspended matter.
In another preference, the described container that stores is the compressible elastomeric plastic containers with sting device being pre-loaded with quantitative liquid/solid/solid-liquid suspended matter；And after compression, elastoplast container sting device breakseal membrane structure integrity, cause storing liquid in containers/solid/solid-liquid suspended matter and enter mixing chamber.
In another preference, when described storage container is closed, the described container that stores is isolation (material i.e. will not be occurred to exchange) with environment.
In another preference, the described capacity storing container is 0.01-200ml.
In another preference, described storage container is disposable container.
In another preference, described storage container is the incompressible disposable plastic container of rigidity.
In another preference, described pipeline connecting valve is valve switch, moving switch or pressure seal.
In another preference, when described connecting tube is closed, described connecting path is in the state of the fluid exchange closing adjacent mixing chambers.
In another preference, described locking device is valve, and described locking device is moving switch, or described locking device is sting device and the diaphragm seal that can puncture.
In another preference, described sting device can puncture diaphragm seal simultaneously, controls to store the connection between container and mixing chamber and closedown.
In another preference, described device also includes an openable salable sample-adding port lid.
In another preference, described field generating unit includes: be positioned at the magnet near each mixing chamber, and shifting magnetic field screening arrangement, and described magnetic field shielding device can move between described permanent magnet and each described mixing chamber.
In another preference, described shifting magnetic field screening arrangement reaches to shield the magnetic field that had of magnet to closing the impact of magnetic bead in detection pipe by the motion of magnetic shield material.
In another preference, described device has magnetic field shielding and open systems, for controlling gathering and the dispersity of magnetic bead in mixing chamber.
In another preference, described field generating unit includes: magnet, and running fix device, and described magnet is fixed on described running fix device, and can fix in the above-mentioned enterprising line slip of running fix device and position.
In another preference, bar magnet is positioned in shiftable track, and can be in turn secured to different mixing chambers, thus is enriched with magnetic bead in different mixing chambers.Preferably, reach to control the bead suspension from different mixing chambers is enriched with and fixes magnetic bead by the motion of bar magnet, thus complete the separation process of magnetic bead and liquid.
In another preference, described device also has mobile magnetic stripe devices, and removable magnetic stripe makes the magnetic bead of absorption shift in connected mixing chamber and be enriched with by changes of magnetic field, so that described magnetic bead moves between different mixing chambers in order.
In another preference, described field generating unit includes the electric magnet of multiple parallel connection.
In another preference, described field generating unit can make each processing unit be in one of two states independently of one another: 1) the shielding magnet impact on mixing chamber completely, magnetic bead can be sufficiently mixed with liquid；2) open magnet impact on mixing chamber, is enriched with magnetic bead, makes magnetic bead separate with liquid.
In another preference, described sensing chamber chip Han solid phase biological.
In another preference, after isolated and purified biological specimen enters sensing chamber, it is fixed in chip and is enriched with and detects.
In another preference, in described device, each stores and contains one or more compositions being selected from lower group in container independently of one another:
I () magnetic bead (magnetic fluid), described magnetic bead is for adsorbing/combine biological specimen to be purified；
(ii) binding soln, described binding soln is for promoting biological specimen to be purified to be incorporated into described magnetic bead；
(iii) wash solution, described wash solution is for going the non-specific binding of the removal of impurity and magnetic microsphere；
And optional (iv) eluent, described eluent is for biological specimen eluting from magnetic bead, and makes magnetic bead separate with the biological specimen of purification；
With optional (v) detection solution, described detection solution is for detecting biological specimen after purification.
In another preference, described detection solution is used for carrying out immunity, nucleic acid, biochemical qualitative or quantitative detection.
In another preference, described detection solution is for reacting with biological specimen, thus carries out chromogenic reaction or fluorescent quantitation detection.
In another preference, described device also includes that sample mix unit, described sample mix unit can make processing unit produce or concussion, so that carrying out liquid transfer between adjacent processing unit.
In another preference, described sample mix unit is used for making above-mentioned closed system tilt.
In another preference, described device is placed in detecting instrument, and has assisted sample to be enriched with and detection by detecting instrument.
In another preference, described detecting instrument is selected from lower group: colour comparatour, fluorescence detector, luminometer.
In another preference, described instrument has pressure seal/switch, is used for controlling to separate or connection adjacent mixing chambers.
In another preference, described instrument has one or more detachable sample collection tube, stores sample after purification.
In another preference, described device also has Photoelectric Detection path, and described Photoelectric Detection path includes one or more detection sensor, qualitatively or quantitatively detects sample after extraction purification.
In another preference, described device also has heater, and described heater is used for making one or more processing unit be in heated condition, makes other processing unit be in non-heated state simultaneously.
In another preference, described test kit also includes sample collecting device.
In another preference, described sample collecting device is trace sample harvester.
In another preference, described trace refers to that the collection capacity of sample is 10ul-5ml liquid sample.
In another preference, described sample collecting device is connected with described storage container or mixing chamber.
In another preference, described sample collecting device is blood-taking device.
In another preference, described test kit also includes anticoagulant.
In another preference, described blood-taking device is finger tip blood-taking device, it is preferred that described finger tip blood-taking device is selected from lower group: finger tip blood taking needle, swab, absorbent paper, smear, capillary tube, dropper.
In another preference, described sample collecting device is scraper or the saliva collector gathering Oral Mucosal Cells.
In another preference, described sample collecting device is for gathering other detection samples such as serum, Excreta, secretions, culture medium.
In another preference, in use, penetrate or thrust sealing lid by syringe, capillary tube or pipet, thus the sample gathered is added described mixing chamber, carry out the extraction of various biomolecule, purification and the detections such as DNA, RNA, albumen, little molecule, cell.
In another preference, sample can be placed in advance in described storage container, opens mode by valve and sample adds totally-enclosed magnetic bead isolated and purified detection equipment.
In another preference, sample can be placed in advance in described in there are the compressible elastomeric plastic containers of sting device, be connected with seal cap sealing, by puncture seal lid mode, sample added totally-enclosed magnetic bead isolated and purified detection equipment.
In another preference, this closed system contains filter membrane and switching device, can carry out solid-liquid separation control.
In another preference, sample can in advance with magnetic bead and combine liquid and mix storage, be subsequently adding described device carry out washing, purification and detection.
In another preference, described device can exist the mixing chamber of more or less numeral, connecting tube further, store container, filter membrane, diaphragm seal, valve switch or pressure seal, biochip, purification specimen collection tube or test cabinet.
Second aspect of the present utility model, it is provided that a kind of biological specimen purification, collection and detection method, described method device as described in this utility model first aspect is carried out, and described method includes step:
(1) provide one to have the first storage container and second and store the first processing unit of container, it is adjacent one another are that described the first storage container and second stores container, and the first described storage container contains magnetic bead and combines buffer, second stores in container containing sample solution；
Second-the n-th processing unit is provided, the storage container of the second-the n-th described processing unit is respectively provided with detergent；
Optionally, it is provided that the (n+1)th processing unit, equipped with eluent in the (n+1)th described processing unit；
Optionally, it is provided that n-th+2-the n-th+m processing unit, n-th described+2-the n-th+m processing unit is respectively provided with test agent；
And be connected step by step between each processing unit；Wherein, n and m is the integer more than 1；
(2) the described first locking device storing container and the second storage container is opened, make described magnetic bead, combine buffer, sample solution enters the mixing chamber of described first processing unit and carries out mixing and reacting, and obtains being combined with the magnetic bead-sample composite thing of sample；
(3) make the first described processing unit be in non-magnetic field environment, open the pipeline connecting valve between described first processing unit and the second processing unit, make the liquid in the first processing unit mixing chamber enter the second processing unit mixing chamber；
(4) the second described processing unit is made to be in magnetic field environment, so that magnetic bead-sample composite thing enters the second processing unit and fixes；
(5) the second processing unit described in lifting the first processing unit described in decline, so that the liquid in the second described processing unit enters the first processing unit；
(6) the pipeline connecting valve between described first processing unit and the second processing unit is closed, open the locking device of described second processing unit, so that the detergent stored in container of the second described processing unit enters the second processing unit mixing chamber and washs described magnetic bead-sample composite thing；
(7) the pipeline connecting valve between described second processing unit and the 3rd processing unit is opened, so that the liquid in the second described processing unit mixing chamber enters the 3rd processing unit mixing chamber；
(8) the 3rd described processing unit is made to be in magnetic field environment, so that described magnetic bead-sample composite thing enters the 3rd processing unit and fixes；
(9) the 3rd processing unit described in lifting the second processing unit described in decline, so that the liquid in the 3rd described processing unit enters the second processing unit；
(10) repeating said steps (6)-(9), make described magnetic bead-sample composite thing through n-1 time wash after, enter the (n+1)th processing unit and and liquid phase separation, obtain scrubbed magnetic bead-sample composite thing.
In another preference, the lifting of each processing unit and decline are carried out by running sample mix unit.
In another preference, described sample solution comprises described biological sample, and can comprise other suitable composition, such as salt, buffer, water, anticoagulant, internal reference product or a combination thereof further.
In another preference, described method is further comprising the steps of:
(11) the pipeline connecting valve between described n-th processing unit and the (n+1)th processing unit is closed, open the locking device of described (n+1)th processing unit, so that the eluent stored in container of the (n+1)th described processing unit enters the (n+1)th processing unit mixing chamber, and carry out eluting, obtain biological specimen；
(12) the (n+1)th described processing unit is made to be in magnetic field environment, the (n+1)th processing unit described in lifting the n-th+2 processing unit described in decline, so that the liquid in the (n+1)th described processing unit enters the n-th+2 processing unit, obtain the biological specimen with Beads enrichment.
In another preference, described method is further comprising the steps of:
(13) the pipeline connecting valve between described (n+1)th processing unit and the n-th+2 processing unit is closed, open the locking device of described n-th+2 processing unit, so that the test agent stored in container of the n-th+2 described processing unit enters the n-th+2 processing unit mixing chamber, and react, obtain test agent-biological specimen product；
(14) test agent-biological specimen product is delivered and reacts by operation step by step that repeat step (6)-(9), until test agent-biological specimen product after completion of the reaction enters the n-th+m processing unit.
Make the test agent in described n-th+m processing unit-biological specimen product enter test device with optional step (15) to test.
In another preference, described method also includes optional step (13a) and (13b):
(13a) the pipeline connecting valve between described (n+1)th processing unit and the n-th+2 processing unit is closed, open all pipeline connecting valves of described n-th+2 to n+m processing unit, so that the biological specimen after purification in the n-th+2 described processing unit distributes into n-th+2 to n+m many processing unit mixing chamber；
(13b) all pipeline connecting valves of described n-th+2 to n+m processing unit are closed, and open the locking device between each processing unit mixing chamber and detectable storage container, make test sample and detectable react, obtain test agent-biological specimen product.
In another preference, described test agent is selected from lower group: paraffin lysate, go crosslinker solution, cracking/combine buffer, cleaning mixture, magnetic bead, eluent, quantitative PCR detection solution, or a combination thereof.
In another preference, in described method, described biological specimen is nucleic acid, protein, little molecule or cell.
Wherein said biological sample is selected from lower group: blood, saliva, oral mucosa, body fluid, root of hair, tissue, serum, feces, body exudates, culture fluid, cell or a combination thereof.
In another preference, described method also includes: uses multiple concurrent testing room/purification of samples collection tube to replace single test cabinet/purification of samples collecting pipe in the device, completes multiple test/sample collection for simultaneously.
In another preference, described method also includes: use multiple analysis magnetic bead or reagent to carry out multiple analysis in the device simultaneously.
In another preference, described device is installed one or more detection sensor, the sample of isolated is tested.
In another preference, described method also includes: be connected with detection equipment by described device, so that the thing containing sample composite of isolated is directly used in detection.
In another preference, multiple described devices are attached with same detection equipment, thus the biomolecule extracted simultaneously and test in one or more sample, such as little molecule, cell, DNA, RNA and/or protein.
In should be understood that in the range of this utility model, can be combined with each other between above-mentioned each technical characteristic of the present utility model and each technical characteristic specifically described in below (eg embodiment), thus constitute new or preferred technical scheme.As space is limited, the most tired at this state.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of device of the present utility model；
Fig. 2 is moving magnet 41, magnetic field shielding and the open design in a preference of the present utility model；
Fig. 3 .1 to Fig. 3 .29 is the detection process (step 1-29) in a preference of the present utility model；
Fig. 4 is the schematic diagram of the device used in embodiment 1；
Fig. 5 is the schematic diagram of the device used in embodiment 2；
Fig. 6 is the schematic diagram of the device used in embodiment 3；
Fig. 7 is the schematic diagram of the device used in embodiment 4.
In figure, the meaning representation of each legend is as follows: mixing chamber: 1,4,7,10,13,16；Connecting tube: 2,5,8,11,14,17；Pipeline connecting valve: 3,6,9,12,15,18, L；Purification of samples collecting pipe or test cabinet: 19；Store container: 20,21,22,23,24,25,26, A, B, C, D, E, F, G；Sample solution: 27；Magnetic fluid: 28；Detergent: 29,30,31；Eluent: 32；Test solution: 33；Locking device: 34,35,36,37,38,39,40；Shifting magnetic field: 41；Magnetic field shielding sheet: 42；Bar magnet is fixed and running fix device: 43；Filter membrane: H, I, J；Salable sample-adding port lid: K.
Detailed description of the invention
The present inventor, through long-term and in-depth study, have developed a kind of totally-enclosed, can carry out liquid feeding, device that biological specimen is purified, shifts, detects or preserves by operating system that solid-liquid mixing, Magneto separate, sample shift and instrument.This small-sized, instrument simple to operate can be the bringing great convenience property such as sample collection, molecule experiments, clinical diagnosis.
Biological specimen purification, collect and detect device
This utility model provides one and can manually carry out totally-enclosed, and the device being purified biological specimen, shift, detect or preserving simple to operate, this equipment is used as instrument and is purified detection process.Above-mentioned device can be not only used for the nucleic acid/albumen in extraction purification biological specimen/little molecule/cell, can also stably preserve and extract complete nucleic acid/albumen/little molecule/cell sample, therefore Large Scale Biology sample collection and preservation, such as common lab or field sample collection etc. it is suitable for.In addition, after using this device and instrument that biological specimen amplifying nucleic acid/albumen/little molecule/cell is carried out fast separating and purifying, further sample qualitative and quantitative testing be can be carried out, medical diagnosis, genetic test, analysis, scientific research and other related application can be widely used for.
The function of device of the present utility model includes liquid feeding, magnetic field enrichment, magnetic field shielding, magnetic field transfer, solid-liquid mixing, magnetic bead transfer, Sample purification, can be purified biological specimen, shifts, detects or preserve in the case of totally enclosed.
Device of the present utility model includes mixing chamber, connecting tube, storage container, magnetic bead, reagent, magnetic field shielding sheet, removable bar magnet, bar magnet fixes and running fix device, seal isolation system, liquid switch valve, sample collection tube or sensing chamber, and can provide rotation, the device shaking or tilting.Device of the present utility model can also replace magnetic field shielding sheet, removable bar magnet, bar magnet to fix and running fix device with mobile electric magnetic field.
Device of the present utility model also can farther include various filter membrane, diaphragm seal.This utility model also can farther include computer, data storage, cooling or heating apparatus.
Device of the present utility model can be hand-manipulated, semi-automatic operation, or full automatic working.
Device of the present utility model includes multiple mixing chamber, makes magnetic bead be fully contacted with liquid by rotating or shaking；In another preference, described mixing chamber is disposable container.In another preference, between mixing chamber by can connect and closing pipe line be connected；Use valve or the connection of other on-off control or close the fluid exchange of adjacent mixing chambers.
In another preference, it is connected by pressure seal between mixing chamber；Pressure seal closure is used to connect or close the fluid exchange of adjacent mixing chambers；
With the connection of on-off control mixing chamber and storage container whether part mixing chamber is connected with one or more independent containers that store, mixing chamber and store and have valve or other on-off control between container, can.
In another preference, part mixing chamber is connected with one or more independent containers that store, and has the spacers such as filter membrane between mixing chamber and storage container, and spacer can efficiently separate solid material and liquid.
Part mixing chamber is connected with one or more independent containers that store, and has diaphragm seal, can be punctured by the pressure etc. that switch valve produces between mixing chamber and storage container.
Part mixing chamber is connected with one or more independence test rooms, and the biological specimen distribution separated in mixing chamber detects to independence test room.
In another preference, it is connected by valve switch closing device between mixing chamber and independence test room；Switch is used to connect or close the fluid exchange between adjacent mixing chambers and independence test room；
In another preference, it is connected by salable flexible pipe between mixing chamber and independence test room；Pressure seal is used to make flexible pipe be in the state of the fluid exchange between connection or closedown mixing chamber and independence test room；
In another preference, part mixing chamber is connected with one or more independence test rooms, has switching device in mixing chamber and between control laboratory, and liquid can be flowed into control laboratory by mixing chamber.
In another preference, control laboratory contains other solid phase carriers, such as biochip.
In another preference, having switching device between multiple storage containers and control laboratory, liquid flows into and out control laboratory in order.
In another preference, this equipment can rotate vertically, carries out the mixed process of liquid and magnetic bead.
In another preference, liquid can use concussion mode to complete with the mixed process of magnetic bead.
In another preference, liquid can use angled manner to complete with the mixed process of magnetic bead.
This equipment may be axially inclined, the liquid in adjacent mixing chambers is made to concentrate on the mixing chamber being positioned at lower section, thus with the Beads enrichment of the mixing chamber being adsorbed in higher position by magnetic field, use valve/pressure seal to close the connecting line between adjacent mixing chambers and lead to part and reach the purpose that magnetic bead separates with liquid.
Described equipment is totally-enclosed pipeline, and device interior environment does not occur mass exchange (such as, liquid, gas, such as the material diffusion such as water, oxygen etc.) with open external environment condition, and sample enters and uses valve/sealing lid mode.
And described sealing lid can control the addition of sample material by valve switch.
And described sealing lid can be injected device or capillary tube penetrates.In use, penetrated or thrust sealing lid by syringe, thus the sample gathered is added described mixing chamber, carry out the extraction of various biomolecule, purification and the detections such as DNA, RNA, albumen, little molecule, cell.Sample has the compressible elastomeric plastic containers of sting device described in can being placed in advance in, and is connected with seal cap sealing, by puncture seal lid mode, sample adds totally-enclosed magnetic bead isolated and purified detection equipment.
Store container
In device of the present utility model, each processing unit can be respectively provided with and one or more independent store container, and described storage container contains following component or a combination thereof:
Magnetic bead: described magnetic bead is for adsorbing/combine nucleic acid/albumen/little molecule/cell to be purified；
Binding soln: described binding soln is for promoting nucleic acid/albumen/little molecule/Cell binding to be purified in described magnetic bead；
Cleaning mixture: for removing the impurity with magnetic microsphere non-specific binding；
Optional eluent: for nucleic acid/albumen/little molecule/cell eluting from magnetic bead, make the nucleic acid/albumen/little molecule/cell separation of magnetic bead and purification；
Optionally detecting solution: for the qualitative and quantitative analysis of purified material, described detection solution can carry out immunity, nucleic acid, biochemical qualitative or quantitative detection, including chromogenic reaction, fluorescent quantitation qualitative detection etc.；
Optional standard substance and internal reference: be used for detecting purification efficiency and quantitative analysis.
Optional pretreating reagent: for sample is carried out pre-treatment.
Described storage container can be disposable container, such as compressibility disposable container.
In another preference, the described container that stores is the plastic containers being pre-loaded with quantitative liquid/solid/solid-liquid suspended matter.
In another preference, described storage container also has locking device, such as, seal valve and/or diaphragm seal, and described sealing valve has open and close two states, and described diaphragm seal can be punctured by the state sealing threshold switch；For example, it is preferable to embodiment in, described diaphragm seal is for sealing aluminium foil, plastics or rubber closure.
In another preference, described diaphragm seal stores liquid in containers/solid/solid-liquid suspended matter before being used for preventing from being punctured and communicates with mixing chamber.
In another preference, the described container that stores is to be pre-loaded with having opening and closing the plastic containers of state of quantitative liquid/solid/solid-liquid suspended matter.
After opening, plastic containers communicate with mixing chamber, cause storing liquid in containers/solid/solid-liquid suspended matter and enter mixing chamber.
In another preference, the described capacity storing container is 0.01-200ml.
Sample collecting device
In preferred implementation of the present utility model, described device also includes the sample collecting device collecting biological sample.
In another preference, described sample collecting device is trace sample harvester, and described trace refers to that the collection capacity of sample is 10ul-5ml liquid sample.
In another preference, described trace refers to the collection capacity≤2ml of sample, and preferably≤1ml, is more preferably≤0.5ml.
In another preference, described sample collecting device is connected with described storage container or mixing chamber, such as, be connected with described sealing lid.
In another preference, described sample collecting device is blood-taking device, it is preferable that described blood-taking device is finger tip blood-taking device, it is preferred that described finger tip blood-taking device is selected from lower group: finger tip blood taking needle, swab, absorbent paper, smear, capillary tube, dropper.In these cases, the described liquid stored in container is possibly together with anticoagulant.
In another preference, described sample collecting device is human body sample collecting device, such as, gather scraper or the saliva collector of Oral Mucosal Cells.In other examples, described sample collecting device is for gathering other detection samples such as serum, Excreta, secretions, culture medium.
In another preference, described sample can in advance with magnetic bead and combine after liquid mixes, add device described in the utility model carry out being enriched with, purification and detection.
Field generating unit
Device of the present utility model also has field generating unit, the design of described field generating unit can use and any one or more described mixing chamber can be made to be in magnetic field environment or in strong magnetic field circumstance, make the design that remaining described mixing chamber is in non-magnetic field or weak magnetic environment simultaneously, for example with moving magnet or moveable magnetic field shielding device, and open design etc. arranges device.By externally-applied magnetic field and withdraw and make magnetic bead assemble in mixing chamber and disperse；Changed by magnetic field position, magnetic bead can be made to shift in connected mixing chamber and be enriched with.
In another preference, bar magnet is fixed and bar magnet can be made to carry out sliding according to fixing pipeline for running fix device and position is fixed, reach to control the bead suspension from different mixing chambers is enriched with and fixes magnetic bead by the motion of bar magnet, thus complete the separation process of magnetic bead and liquid.
In another preference, magnetic field shielding sheet and mobile device reach to shield magnetic field that magnet had to closing the impact of magnetic bead in detection pipe by the motion of magnetic shield material.
In another preference, (being such as stuck in described track makes it not come off) it is positioned in shiftable track with bar magnet, and described bar magnet can be automatically or manually transfer, it is in turn secured to different sample mixing chambers, thus the magnetic bead in different sample mix rooms is enriched with.
Described field generating unit also can use multiple parallel connection, and the electric magnet that can be switched on or off individually forms.
In another preference, magnetic field control system positions and shifting magnetic field for what double-deck magnetic shield material (such as steel disc etc.) and magnet were constituted.The magnet two states on sample mix room is reached: 1 by the relative motion of magnetic shield material) the shielding magnet impact on sample mix room completely, magnetic bead can be sufficiently mixed with liquid；2) open magnet impact on sample mix room, is enriched with magnetic bead, makes magnetic bead separate with liquid.
Whole closing appliance automation mechanized operation and the instrument of detection
Whole closing appliance of the present utility model can also be connected with the instrument of automation mechanized operation and detection, and such as, described device can be positioned on detecting instrument, or the outlet of afterbody processing unit is connected with instrument.
In another preference, the most not having sample mix unit, the mixing of sample realizes axial backmixing by described instrument, shakes or tilt, or described instrument has compressor, so that storing solution in container to flow into mixing chamber.
In another preference, described instrument also has one or more detachable sample collection tube, stores sample after purification.
In another preference, described instrument also has Photoelectric Detection path, including one or more detection sensors, sample after extraction purification is carried out qualitative and quantitative detection.
The biomolecule needing purification or detection includes any biomolecule, such as, nucleic acid, cell, protein and little molecule.Biological sample for purification includes any biological sample, such as, blood, saliva, oral mucosa, body fluid, root of hair, tissue, serum, feces, body exudates, and culture fluid etc..
Test is included in any suitable test as known in the art and analysis method.Such as, for nucleic acid, these tests and analysis include amplification, order-checking etc.；For protein, polypeptide, cell and little molecule, test and analysis includes that size determines, immunoassay, functional analysis, spectrum analysis, mass spectral analysis etc..
Fig. 1 is a kind of schematic diagram of device of the present utility model, and wherein, described device includes:
The most mixing chambers, respectively Isosorbide-5-Nitraes, 7,10,13, and 16 modules.The quantity of mixing chamber can be increased or decreased.Mixing chamber can be level one row and adjacent.Mixing chamber can also be configured in other suitable manners, as vertically adjacent to.Mixing chamber can be made with any applicable material, and in another preference, mixing chamber is disposable plastic containers.
The most connecting tubes, respectively 2,5,8,11,14, and 17 modules.The quantity of connecting tube can be increased or decreased.Each connecting tube connects two adjacent mixing chambers, or connects mixing chamber and purification of samples collecting pipe or test cabinet.In another preference, connecting tube is the pipe-line system that can carry out on an off switching.Pipe or switch can be any suitably sized, and made by any suitable method as known in the art.
The most storage containers, respectively 20,21,22,23,24,25, and 26 modules.The quantity storing container can be increased or decreased.In another preference, storage container can be controlled with valve switch.One or more storage containers can be installed in the top of some mixing chambers.Each storage container is to contact with the mixing chamber direct physical below it, but open front liquid is not directly contacted with.When there being more than one storage container to be arranged on same mixing chamber, they can be adjacent one another are.Storage container can also be located at other suitable positions.Each storage container includes switching device.Wherein, one or more storage containers include magnetic bead.In another preference, storage container is disposable container.In some other embodiments, storage container is a disposable container that can open closedown.In another preference, storage container is the plastic containers being preinstalled with a certain amount of liquid, solid or solid-liquid suspensions.In another preference, the volume of storage container is about 0.01 milliliter to about 200 milliliters.Storage container can be manufactured by any suitable material and method, and can be any suitably sized and keep any suitable volume.Storage container can be stored in different local (such as, preserve) by different temperature, and is connected to mixing chamber or test cabinet before operation.
The most the valve systems that can carry out open and close state, respectively 34,35,36,37,38,39,40 modules.The quantity of switch can be increased or decreased.Controlled valve is between mixing chamber and one or more storage container.Described biological sample enters system by using valve 34, it is also possible to enter system from other one or more valves and storage container.In another preference, by sealing/connecting pipeline to sample can be added storage container 20 with syringe, capillary tube or pipet, or be directly appended to mixing chamber 1.
The most pipeline connecting valves, respectively 3,6,9,12,15,18 modules.The quantity of pipeline connecting valve can be increased or decreased.Each pipeline connecting valve is between adjacent mixing chamber, mixing chamber and purification of samples collecting pipe and/or mixing chamber and sensing chamber.Pipeline connecting valve is suitable to open or close connecting tube, to allow or to forbid the fluid exchange between adjacent mixing chamber or mixing chamber and purification of samples collecting pipe/test cabinet.Pipeline connecting valve can be made by any suitable material and method, and can be any suitably sized.Pressure seal can be used to replace switch.
6. purification of samples collecting pipe and sensing chamber 19.Sample collection tube or the test cabinet 19 of purification are adjacent with last mixing chamber.Purification of samples collecting pipe or test cabinet can be located at any suitable position, such as less than or higher than mixing chamber, and can have any appropriate number of sample collection tube/sensing chamber.In another preference, transfer to one or more purification of samples collection tube from the described biomolecule of biological sample purification from mixing chamber, to complete Sample Purification on Single.In another preference, transfer to one or more test cabinet for testing from the described biomolecule of biological sample purification from mixing chamber.In another preference, valve switch is used for connecting mixing chamber and purification of samples collecting pipe/sensing chamber so that this purification of samples can be allocated at multiple purification of samples collecting pipes or test cabinet.In another preference, the connecting tube that mixing chamber and purification of samples collection tube/test cabinet use is a salable flexible pipe.In another preference, mixing chamber is connected by switch with purification of samples collection tube/test cabinet.Purification of samples collecting pipe or test cabinet can be produced by any suitable material and method, and can be any suitably sized.
7. sample solution 27, are placed on storage container 20.Sample solution comprises described biological sample, and can comprise other suitable composition, such as salt, buffer, water, anticoagulant etc. further.
8. magnetic fluid 28: include magnetic bead and combine buffer, are positioned over storage container 21.Magnetic bead can also be placed on single storage in container with combining buffer.Magnetic bead and combine buffer can be commercially.According to different application programs (biomolecule that i.e. purification is different), one it will be appreciated by those skilled in the art that and uses different magnetic bead and combine buffer, it is possible to add lysate to help cell lysis.Magnetic bead is used for purifying biological molecule, including nucleic acid, protein, little molecule, and cell.Under proper condition, biomolecule is combined on magnetic bead, and by magnetic field separation magnetic bead, uncombined impurity is retained in solution being removed.Be combined on magnetic bead biomolecule can elute from magnetic bead with suitable buffer subsequently, by magnetic field separation magnetic bead, so that it may obtain purification biomolecule.
9. detergent 29,30,31, it is respectively placed in storage container 22,23,24.Wash solution can be any suitable wash solution commercially, for washing magnetic bead, the impurity of removal non-specific binding.Eluent 32, is placed in storage container 25.Eluent can be any suitable eluting solution commercially, for biomolecule eluting from magnetic bead.
10. test solution 33, are placed in storage container 26, store container 26 and are connected with a mixing chamber.In another preference, test solution can also be stored in the storage container being joined directly together with test cabinet, so test solution can be added directly in test cabinet.It will be appreciated by those skilled in the art that, can be according to specific test, differently configured testing liquid, it is also possible to commercially.Testing liquid is any suitable testing liquid, it is also possible to be dried powder.
11. shifting magnetic fields 41, removable bar magnet, mixing chamber 1,4,7,10,13,16, purification of samples collecting pipe or test cabinet 19 can be positioned over, or sealing unit is not produced the position of magnetic field impact.In another preference, shifting magnetic field is a part rather than a part for sealing device for instrument.The position in magnetic field can change, thus adsorb magnetic bead and allow magnetic bead to be suspended, shift and be enriched in certain mixing chamber.
Fig. 2 shows moving magnet 41, magnetic field shielding and the open design in another preference of the present utility model.
Fix including magnetic field shielding sheet 42 and bar magnet and running fix device 43.
Magnetic field shielding sheet 42: mixing chamber 1,4,7,10,13,16, purification of samples collecting pipe or test cabinet 19, armoured magnetic field can be positioned over, mobile bar magnet can be eliminated on the impact of magnetic bead in sealing device.
Bar magnet is fixed and running fix device 43: has slip pipeline and makes mobile bar magnet move in different mixing chamber sides, has position Fixed Design and makes mobile bar magnet be securable to position, specific blend room enrichment magnetic bead.
Fig. 3 .1 to Fig. 3 .29 shows the detection process (step 1-29 is successively as shown in Fig. 3 .1 to Fig. 3 .29) in another preference of the present utility model.
1. initial state
2. opening the valve switch 34,35 storing container 20,21, make described biological sample and magnetic bead, combine buffer mixing, by rotating, shaking or tilt to make biological sample and magnetic bead to be sufficiently mixed, magnetic bead is combined with molecule to be purified.
3. open fluid path switch 3, connect mixing chamber 1 and 4 by connecting tube 2；Mobile for slip magnetic stripe 41 is placed on mixing chamber 4, fixing magnetic stripe position；Remove magnetic field shielding sheet, make magnetic field shielding sheet be placed in mixing chamber 7,10 times；It is enriched with magnetic bead by magnetic field；
4. inclined seal unit, flows liquid into mixing chamber 1；
5. closing liquid channel selector 3, cuts out connecting tube 2, closes the liquid communication between mixing chamber 1 and 4；
6., by sealing unit Reversion Level position, the mobile magnetic stripe 41 that slides is placed and is fixed on mixing chamber 7；
7. open storage container 22 Valve controlling switch 36, make magnetic bead and cleaning mixture mixing, by rotating, shake or tilt washing magnetic bead.
8. open fluid path switch 6, connect mixing chamber 4 and 7 by connecting tube 5；
9. remove magnetic field shielding sheet, make magnetic field shielding sheet be placed in mixing chamber 10,13 times；
Shifting magnetic field 41 is enriched with magnetic bead at mixing chamber 7；
10. inclined seal unit, flows liquid into mixing chamber 4；
11. close valve switch 6, close connecting tube 5, close the liquid communication between mixing chamber 4 and 7；
12-22. repeat the above steps washs magnetic bead in mixing chamber 7,10,13, goes the removal of impurity；
After 23. washings, enrichment with magnetic bead is in mixing chamber 13, closes connecting tube 12；
24. open storage container 25 valve switch 39, make magnetic bead and eluent 32 mix, the biomolecule being originally bound on magnetic bead are eluted by rotating, shaking or tilt.
25. shifting magnetic fields 41 are enriched with magnetic bead to mixing chamber 13.
Inclined seal assembly, opens channel selector part 15, connects mixing chamber 13 and 16 by connecting tube 14, makes liquid flow into mixing chamber 16 from mixing chamber 13.
26. close channel selector 15, close connecting tube 14, close the liquid communication between mixing chamber 13 and 16.
27. open storage container 26 valve switch 40, make the biomolecule of purification pass through to rotate, tilt or vibrate to mix with test solution 33.
28. open channel selector valve 18, connect mixing chamber 16 and sensing chamber 19 by connecting tube 17；Then keep black box to tilt, flow liquid into sensing chamber 19；
29. pass through a detection sensor (not shown) being arranged on instrument for analyzing and testing.
29. or, test can also be carried out after completing following steps: closing presure sealing member 18, close connecting tube 17, sealing unit returns to a specific position.
If simply collecting the sample of purification, it is not required to detection, then after the 24th step, shifting magnetic field 41 is enriched with magnetic bead to mixing chamber 13, then directly the eluent containing purification of samples being transferred to sample collection tube 19, collecting pipe 19 can disconnect from whole unit, and add a cover and store.
Any those of ordinary skill in the art it will be appreciated that during above-mentioned detection process, or modified above-mentioned detection, can manually, automatically, semiautomation operation.
Fig. 1, Fig. 2 and Fig. 3 .1 to Fig. 3 .29 provides the general description of some embodiment of the present utility model.For a specific extraction and/or the detection method of test biomolecule (such as little molecule/cell, nucleic acid and/or protein), the setting of whole system, assembly and program can include but not limited to respective change: some mixing chamber can add, walks around or omit；Store container and can store the different reagent for special test；Some storage containers are probably sky；Can remove on some mixing chambers or test cabinet or add one or more storage container etc.；In addition to valve and other switches, filter membrane can be had to isolate between mixing chamber and storage container, reach the purpose of solid phase and liquid phase separation, can be used for solid biologic sample pre-treatment procedure.
Assembly of the present utility model can have other modification, includes but not limited to: uses multiple concurrent testing room/purification of samples collection tube to replace single test cabinet/purification of samples collecting pipe 19, for completing multiple test/sample collection simultaneously, reaches high flux；Can fix and carry out example enrichment and detection containing solid phase carriers such as biochips in mixing chamber and sensing chamber；Multiple analysis magnetic bead or reagent can also be used to carry out multiple analysis simultaneously；And one or more detection sensors can be installed on an instrument for multiple concurrent testings.
In addition, multiple sealing devices can be installed in an equipment for extracting and test the biomolecule in one or more sample simultaneously, such as little molecule, nucleic acid, protein and/or cell, include but not limited to: parallel extraction and detection DNA and RNA simultaneously, or extract simultaneously and detect DNA, RNA and/or protein, etc..
Any buffer and solution can use switch or by the diaphragm seal that can puncture, and join in described storage container, and the whole seal member that need not break a seal.
Any buffer and solution can use injection or pipet by a diaphragm seal that can puncture, and join in described storage container, and the whole seal member that need not break a seal.
Below in conjunction with specific embodiment, this utility model is expanded on further.Should be understood that these embodiments are merely to illustrate this utility model rather than limit scope of the present utility model.The experimental technique of unreceipted actual conditions in the following example, generally according to normal condition, or according to the condition proposed by manufacturer.Unless otherwise indicated, otherwise percentage ratio and number are calculated by weight.
Embodiment 1
Extract blood DNA and detect device and the flow process of hepatitis B virus:
Device used in embodiment 1 is shown in accompanying drawing 4.
1. the diaphragm seal A-blood sample that can puncture by can puncture seal film A switch add system.
2. storage container B includes internal standard 200 microlitre.
3. storage container C includes magnetic bead and cracking/combine buffer: 1mg magnetic bead and 1.5ml crack/combine buffer.
4. storage container D includes cleaning mixture I:1ml.
5. storage container E includes cleaning solution II: 1ml.
6. storage container F includes cleaning solution II I:1ml.
7. storage container G includes eluent 100 μ L.
8. storage container H includes quantitative PCR detection liquid 100 μ L.
Magnetic bead, cracks/combines buffer, internal standard, cleaning mixture I, cleaning solution II, cleaning solution II I, eluent, and quantitative PCR detection solution (PCR buffer, HBV primed probe, Taq polymerase) is commercially available prod.
By aforementioned extraction process (seeing Fig. 3 .1 to Fig. 3 .29), after extraction purification DNA, remix DNA and the quantitative PCR detection solution of purification, transfer the solution into test cabinet 19, by typical nucleic acid amplification and measure hepatitis B virus gene.On test cabinet 19 side, heater/cooler is installed on instrument, for carrying out the temperature that quantitative PCR reaction cycle provides suitable.One detection sensor, such as photometer, be also mounted at test cabinet 19 side, to monitor real-time fluorescence transmitting.According to the test result of this instrument record, the content of hepatitis B virus gene can be calculated, for medical diagnosis.
Embodiment 2 extracts device and the flow process of blood DNA
Accompanying drawing 5 is shown in by the schematic diagram of the device that embodiment 2 is used.
1. storage container A, including needing the biological sample of purification, 200 μ l.
2. storage container B, including magnetic bead and cracking/combine buffer: 1mg magnetic bead and 1.5ml crack/combine buffer.
3. storage container C includes cleaning mixture I:1ml.
4. storage container D includes cleaning solution II: 1ml.
5. storage container E includes cleaning solution II I:1ml.
6. storage container F includes eluent 100 μ L.
Magnetic bead, cracks/combines buffer, cleaning mixture I, cleaning solution II, wash solution III, and eluent is commercially available prod.
By step 1-24 during aforementioned extraction (seeing Fig. 3 .1 to Fig. 3 .24), DNA extracts from blood and is eluted out, shifting magnetic field is enriched with magnetic bead to mixing chamber 13, the sample of purification is collected in purification of samples collecting pipe 19, sample collection tube 19 can be taken off from whole unit, preserves after closeing the lid.
Embodiment 3 is extracted and uses the device of ELISA detection protein
The device that embodiment 3 is used is shown in accompanying drawing 6.
1. storage container A comprises the plasma sample containing antigen to be analyzed.
2. storage container B includes magnetic bead and combines buffer.
3. storage container C includes that cleaning mixture I is to remove the protein of non-specific binding.
4. storage container D includes the solution containing traget antibody.
5. storage container E includes that cleaning solution II is to remove the antibody of non-specific binding.
6. storage container F includes buffer.
7. storage container G includes colour developing or luminescent solution.
Antibody can be covalently attached to enzyme, or itself can be detected by the second antibody of link enzyme.In this example, antibody is covalently attached to a kind of enzyme.When substrate is by enzyme catalysis, produces a visible signal, antigen content in the sample can be calculated.
Magnetic bead, in conjunction with buffer, cleaning mixture I, antibody, cleaning solution II, substrate solution is all commercially available product.Plasma sample, magnetic bead and combine buffer and first mix (heater or cooler may be mounted to that this device) at a suitable temperature at mixing chamber 1, antigen is combined on magnetic bead.Mobile magnetic bead, to mixing chamber 4, uses cleaning mixture I to remove the protein of non-specific binding.Then transfer magnetic bead is to mixing chamber 7, and the solution at a suitable temperature with containing antibody hatches (heater or cooler may be mounted to that this device).After this, mobile magnetic bead is to mixing chamber 10, and washs magnetic bead by cleaning solution II.Transfer magnetic bead is to mixing chamber 13 the most further, and mixes with substrate solution.Magnetic bead and solution are moved on to sensing chamber 19 in the lump, and hatch (heater or cooler may be mounted to that this device) at a suitable temperature.It is arranged on the detection sensor on test cabinet 19 side, can be with the level of detection by quantitative antigen.
Embodiment 4 extracts device and the flow process of paraffin embedding sample FFPE DNA
Accompanying drawing 7 is shown in by the schematic diagram of the device that embodiment 4 is used.
1. storage container A, including paraffin lysate, 600 μ l.
2. storage container B, removes crosslinker solution, 600 μ l.
3. storage container C: include magnetic bead and cracking/combine buffer: 1mg magnetic bead and 1.5ml crack/combine buffer.
4. storage container D includes cleaning mixture I:1ml.
5. storage container E includes cleaning solution II: 1ml.
6. storage container F includes cleaning solution II I:1ml.
7. storage container G includes eluent 200 μ L.
8. filter membrane H, I, J.
The most salable sample-adding port lid K.
Magnetic bead, cracks/combines buffer, cleaning mixture I, cleaning solution II, wash solution III, and eluent is commercially available prod.
1) paraffin sample is placed in mixing chamber 1 by salable sample-adding port lid K, open storage container A, mixing (heater may be mounted to that this device) dewaxing at a suitable temperature, inverted device, liquid is made to return storage container A, solid microstructure section is stopped by filter membrane H stays mixing chamber 1, closes storage container A.
2) opening storage container B, crosslinking is gone in mixing (heater may be mounted to that this device) at a suitable temperature.
3) tilting gearing, opens coupling cock L, makes to flow into mixing chamber 4 containing nucleic acid liquid, and solid microstructure section is stopped by filter membrane J stays mixing chamber 1, closes coupling cock L.
4) combining liquid containing magnetic bead to mix at mixing chamber 4 with containing nucleic acid liquid, then transfer magnetic bead is to mixing chamber 7, uses cleaning mixture I washing.After this, mobile magnetic bead is to mixing chamber 10, and washs magnetic bead by cleaning solution II.The most mobile magnetic bead is to mixing chamber 13, and washs magnetic bead by cleaning solution II I.Then transfer magnetic bead is to mixing chamber 16, and mixes with eluent.Magnetic bead stays mixing chamber 16, is moved to collecting chamber 19 containing nucleic acid solution, and the sample of purification is collected in purification of samples collecting pipe 19, and sample collection tube 19 can be taken off from whole unit, preserves after closeing the lid.
Unless otherwise defined, all technology used herein and scientific terminology have the implication that those of ordinary skill is generally understood that in this utility model art.The use of certain methods and material is described in this utility model；But other suitable methods and material as known in the art are used as.Described material, method and example are merely illustrative, it is no intended to limit.
The all documents mentioned at this utility model are incorporated as reference the most in this application, are individually recited as with reference to like that just as each document.In addition, it is to be understood that after having read above-mentioned teachings of the present utility model, this utility model can be made various changes or modifications by those skilled in the art, these equivalent form of values fall within the application appended claims limited range equally.
Claims (10)
1. one kind is extracted and the device of detection biological specimen, it is characterised in that described device includes:
(a) multiple processing unit, the most each processing unit includes:
(a1) one or more storage containers, described storage container can store liquid, solid or solid-liquid suspended matter；
(a2) and the adjacent mixing chamber of described storage container, described mixing chamber is used for making magnetic bead contact with liquid；
(a3) locking device between described storage container and mixing chamber, described locking device can make to be between storage container and the mixing chamber of described processing unit connection or not connected state；
B (), between adjacent mixing chambers, for connecting the connecting tube of adjacent processing units, described connecting tube has pipeline connecting valve, described pipeline connecting valve can regulate and make described connecting tube be in the state of being turned on and off；
(c) field generating unit, described field generating unit is positioned near described multiple mixing chambers, and single described mixing chamber can be made to be in magnetic field environment, make remaining described mixing chamber be in non-magnetic field environment simultaneously, or make single described mixing chamber be in strong magnetic field circumstance, and other mixing chamber is made to be in more weak magnetic field environment；
With optional (d) sample collection tube and/or sensing chamber.
2. device as claimed in claim 1, it is characterized in that, described field generating unit includes: be positioned at the magnet near each mixing chamber, and shifting magnetic field screening arrangement, and described magnetic field shielding device can move between described magnet and each described mixing chamber.
3. device as claimed in claim 1, it is characterized in that, described field generating unit includes: magnet, and running fix device, described magnet is fixed on described running fix device, and can fix in the above-mentioned enterprising line slip of running fix device and position.
4. device as claimed in claim 3, it is characterised in that bar magnet is positioned in shiftable track, and can be in turn secured to different mixing chambers, thus is enriched with magnetic bead in different mixing chambers.
5. device as claimed in claim 1, it is characterised in that described field generating unit includes the electric magnet of multiple parallel connection.
6. device as claimed in claim 1, it is characterised in that described sensing chamber chip Han solid phase biological.
7. device as described in claim 1, it is characterised in that there is also filter membrane between described storage container and mixing chamber；And/or
Filter membrane is there is also between described adjacent connecting tube.
8. device as described in claim 1, it is characterised in that described device also includes that sample mix unit, described sample mix unit can make processing unit produce or concussion, so that carrying out liquid transfer between adjacent processing unit.
9. device as described in claim 1, it is characterised in that also having heater, described heater is used for making one or more processing unit be in heated condition, makes other processing unit be in non-heated state simultaneously.
10. device as described in claim 1, it is characterised in that described device also includes sample collecting device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620006790.9U CN205570357U (en) | 2016-01-04 | 2016-01-04 | Device of biological sample is drawed and detected |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620006790.9U CN205570357U (en) | 2016-01-04 | 2016-01-04 | Device of biological sample is drawed and detected |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205570357U true CN205570357U (en) | 2016-09-14 |
Family
ID=56881938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620006790.9U Active CN205570357U (en) | 2016-01-04 | 2016-01-04 | Device of biological sample is drawed and detected |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205570357U (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105562132A (en) * | 2016-01-04 | 2016-05-11 | 上海医脉赛科技有限公司 | Device for extracting and detecting biological sample |
| CN110208522A (en) * | 2019-06-27 | 2019-09-06 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic particle shines micro-fluidic chip and reaction method |
| CN110208524A (en) * | 2019-06-27 | 2019-09-06 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic particle shines micro-fluidic chip and reaction method |
| CN110252435A (en) * | 2019-06-27 | 2019-09-20 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic particle shines micro-fluidic chip and reaction method |
| WO2021188562A1 (en) * | 2020-03-17 | 2021-09-23 | Detect, Inc. | Seal component for a rapid diagnostic test |
| CN113484121A (en) * | 2021-06-28 | 2021-10-08 | 同济大学 | Separation and purification device and separation and purification method based on dynamic magnetic field |
| US20210403856A1 (en) * | 2018-09-28 | 2021-12-30 | Octane Biotech Inc. | Magnetic separation |
| WO2022098815A1 (en) * | 2020-11-06 | 2022-05-12 | Detect, Inc. | Rapid diagnostic test |
| CN115501921A (en) * | 2020-06-16 | 2022-12-23 | 萨尼舒尔股份有限公司 | Closed fluid receiving and sampling container |
-
2016
- 2016-01-04 CN CN201620006790.9U patent/CN205570357U/en active Active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105562132A (en) * | 2016-01-04 | 2016-05-11 | 上海医脉赛科技有限公司 | Device for extracting and detecting biological sample |
| CN105562132B (en) * | 2016-01-04 | 2018-06-26 | 上海医脉赛科技有限公司 | A kind of device extracted and detect biological sample |
| US20210403856A1 (en) * | 2018-09-28 | 2021-12-30 | Octane Biotech Inc. | Magnetic separation |
| CN110208522A (en) * | 2019-06-27 | 2019-09-06 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic particle shines micro-fluidic chip and reaction method |
| CN110208524A (en) * | 2019-06-27 | 2019-09-06 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic particle shines micro-fluidic chip and reaction method |
| CN110252435A (en) * | 2019-06-27 | 2019-09-20 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic particle shines micro-fluidic chip and reaction method |
| WO2021188562A1 (en) * | 2020-03-17 | 2021-09-23 | Detect, Inc. | Seal component for a rapid diagnostic test |
| EP4121210A4 (en) * | 2020-03-17 | 2023-10-18 | Detect, Inc. | Seal component for a rapid diagnostic test |
| CN115501921A (en) * | 2020-06-16 | 2022-12-23 | 萨尼舒尔股份有限公司 | Closed fluid receiving and sampling container |
| WO2022098815A1 (en) * | 2020-11-06 | 2022-05-12 | Detect, Inc. | Rapid diagnostic test |
| CN113484121A (en) * | 2021-06-28 | 2021-10-08 | 同济大学 | Separation and purification device and separation and purification method based on dynamic magnetic field |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205570357U (en) | Device of biological sample is drawed and detected | |
| CN105562132B (en) | A kind of device extracted and detect biological sample | |
| US20220176370A1 (en) | Method for purifying and testing biomolecules from biological samples | |
| US9028777B2 (en) | Automated cellular material preparation | |
| US7785535B2 (en) | Sample multiprocessing | |
| JP4410252B2 (en) | Fluid sample processing equipment | |
| US20060166347A1 (en) | Sample preparation devices and methods | |
| US20100129827A1 (en) | Method and device for sample preparation control | |
| US20070125942A1 (en) | Apparatuses, systems and methods for isolating and separating biological materials | |
| US20110244466A1 (en) | Nucleic acid testing device and method | |
| JP2009500011A (en) | Cartridge for automated medical diagnosis | |
| EP2143491A1 (en) | Device for analysing a chemical or biological sample | |
| WO2021254519A1 (en) | Sample processing and detection apparatus and application thereof | |
| US20160017272A1 (en) | Devices and Methods for Purification, Detection and Use of Biological Cells | |
| RU84381U1 (en) | DEVICE FOR AUTOMATED ISOLATION OF NUCLEIC ACIDS | |
| EP2167932B1 (en) | Sample preparation apparatus | |
| US11927600B2 (en) | Fluidic bridge device and sample processing methods | |
| JP2021047170A (en) | Microfluidic device for preparing and analyzing biological sample | |
| CN114085743A (en) | Full-automatic nucleic acid processing amplification detection method and magnetic control detection test tube | |
| JP2021035353A (en) | Microfluidic device including amplification reaction chamber | |
| CN115427147A (en) | Apparatus and method for genetic analysis of plant material at a remote test site |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180607 Address after: 314100 3106 room, 3 room, 3110 room, 4 layer, 3 building, 568 Jinyang East Road, Luo Xing street, Jiashan County, Jiaxing, Zhejiang. Patentee after: Jiaxing medical Mai Sai Technology Co., Ltd. Address before: 201201 Pudong New Area, Shanghai Zhangjiang east area 528, 11, a 2 layer. Patentee before: Emerging Theropeuties (Shanghai) Co.,Ltd. |
|
| TR01 | Transfer of patent right |