CN205368202U - Kit of utilization function ization bacterium magnetism granule purification igA albumen - Google Patents

Kit of utilization function ization bacterium magnetism granule purification igA albumen Download PDF

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Publication number
CN205368202U
CN205368202U CN201620138744.4U CN201620138744U CN205368202U CN 205368202 U CN205368202 U CN 205368202U CN 201620138744 U CN201620138744 U CN 201620138744U CN 205368202 U CN205368202 U CN 205368202U
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reagent
reagent bottle
kit
magnetic particles
test kit
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Expired - Fee Related
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CN201620138744.4U
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Chinese (zh)
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李勇
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Individual
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Individual
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Abstract

The utility model provides a kit of utilization function ization bacterium magnetism granule purification igA albumen, which comprises a box body and a box cover, by last tool holder, equipment box and the pull box of having set gradually under to in the box body, magnetic means has been placed in the equipment box, be equipped with the diaphragm in the tool holder, evenly be equipped with the tube hole on the diaphragm, be located in the tool holder the perpendicular below of diaphragm is equipped with breakage -proof gasket, be equipped with the platform in the pull box, be equipped with reagent bottle holder on the platform, the last fixed bolster that is equipped with of reagent bottle holder, it has the vestibule to open on the fixed bolster. This kit structure is simple, not only can save reagent, and magnetic means can also be saved to this kind of kit in addition, and when the magnetic resolution is tested, can directly take out the kit and detect, convenient to use, in addition, reagent is separately placed with the instrument in this kit, and the instrument is also separately placed with the utensil, and reagent cross contamination in preventing to test has improved detection efficiency, has improved the degree of accuracy that detects, and the practicality is strong.

Description

A kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen
Technical field
This utility model relates to biological technical field, particularly to a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen.
Background technology
Based on magnetic micro-nano granules (magnetic bead) magnetic separation technique from the eighties in last century be born since, more and more applied in the field such as life science and Clinical detection.Beads enrichment technical operation is simple, can efficiently, quickly realize separating effect;This technology manual operation requires low, also can be applicable to automation equipment operation very well.Bacterial magnetic particles is a kind of natural biomagnetism nano material, has the advantage that some synthetic magnetic beads are incomparable.Recombinant protein A is widely applied at present to carry out extraction purification IgA albumen (, Immunoglobulin reagents extraction purification in immunoglobulin, particularly plasma protein preparation production) in scientific research and commercial production.Functionalization recombinant protein A bacterial magnetic particles, is jointly hatched by recombinant protein A and bacterial magnetic particles to form, and it has low cost, the feature of high benefit, compares filled column purification process easy, and adaptability is wider.
Magnetic separation technique be with nanometer or the magnetic particle of microsize grade for carrier, in conjunction with the special affinity characteristic that the protein in magnetic particle surface provides, under the oriented control adding magnetic field, by affine absorption, cleaning, desorption operations, a step can be separated to target molecule from complicated living things system, there is the many merits such as Magnetic Isolation absorption high specific simple and convenient, affine and hypersensitivity.This technology manual operation requires low, also automation equipment operation can be applicable to very well, current magnetic separation technique all use mostly test kit hold reagent, but existing test kit is the common box for holding reagent, it is not specifically designed to test kit special in magnetic separation technique, such as existing patent publication No. is the magnetic microparticle chemiluminescence immunity detection reagent of a kind of Clenbuterol disclosed in CN203929780U, this test kit only achieves depositing of reagent, but this test kit can not be used directly to experiment detection, and this test kit is for magnetic separation technique inapplicable, therefore, it is badly in need of exploitation one and can preserve reagent for a long time, reagent is facilitated to transport, and the detection kit of experiment detection can be used directly to.
Utility model content
In order to solve in prior art but without be specifically designed to utilize functionalization bacterial magnetic particles purification IgA albumen test kit, existing test kit is only capable of realizing storage in the reagent short time, experiment detection can not be used directly to, and test kit inconvenience long-distance transportation, the problems such as poor practicability, this utility model provides a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen.
The concrete technical scheme of this utility model is as follows:
This utility model provides a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen, including the lid that box body and described box body are hinged, instrument cases, appliance case and drawing box it is disposed with from top to bottom in described box body, described appliance case and described drawing box are all slidably connected with described box body, are placed with magnetic means in described appliance case;Being provided with transverse slat in described instrument cases, described transverse slat is uniformly provided with pore, the vertical lower being positioned at described transverse slat in described instrument cases is provided with breakage-proof pad;
Being provided with platform in described drawing box, described platform is provided with reagent rack, and described reagent rack is provided with fixing pad, described fixing pad has vestibule, places the first reagent bottle, the second reagent bottle, the 3rd reagent bottle in described vestibule respectively;It is contained with balance activation buffer in described first reagent bottle, is contained with elution buffer A in described second reagent bottle, in described 3rd reagent bottle, is contained with recombinant protein A functionalization bacterial magnetic particles reagent.
Further, described reagent rack includes base plate, case and is fixed on the swingle on described base plate, has through hole in the middle part of described case, described case by described through hole cross-under on described swingle.
Further, being divided into many sub-boxes by baffle plate in described case, be equipped with fixing pad in multiple described sub-boxes, described first reagent bottle, described second reagent bottle and described 3rd reagent bottle are individually positioned in different described sub-boxes.
Further, described instrument cases medial wall is provided with every platform, described transverse slat two ends fixed described on platform, be perpendicular to described transverse slat in described instrument cases and be provided with stringer board, described instrument cases are divided into instrument by described stringer board and hold district and vessel hold district.
Preferably, on described transverse slat, corresponding described pore is respectively provided with slot tag, is used for pasting reagent label in described slot tag.
Preferably, it is equipped with handle outside described appliance case and described drawing box.
The beneficial effects of the utility model are as follows: the reagent cartridge configuration that this utility model provides is simple, can not only storing reagent, and this test kit can also store magnetic means, when Magnetic Isolation is tested, test kit can be directly taken out detect, easy to use, in addition, this seminal plasma fructose detection kit is separated with instrument, instrument is also separated with utensil, it is prevented that reagent cross-contamination in experiment, improves detection efficiency, improve the accuracy of detection, practical.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen described in embodiment 1;
Fig. 2 is the structural representation of a kind of seminal plasma fructose detection kit frame utilizing functionalization bacterial magnetic particles purification IgA albumen described in embodiment 2;
Fig. 3 is the top view of a kind of seminal plasma fructose detection kit frame utilizing functionalization bacterial magnetic particles purification IgA albumen described in embodiment 2;
Fig. 4 is the top view of a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen described in embodiment 2;
Fig. 5 is the structural representation of a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen described in embodiment 2.
Wherein: 1, box body;2, lid;3, instrument cases;4, appliance case;5, drawing box;6, magnetic means;7, transverse slat;8, pore;9, breakage-proof pad;10, platform;11, reagent rack;12, fixing pad;13, vestibule;14, the first reagent bottle;15, the second reagent bottle;16, the 3rd reagent bottle;17, base plate;18, case;19, swingle;20, baffle plate;21, sub-box;22, every platform;23, stringer board;24, instrument holds district;25, vessel hold district;26, slot tag;27, handle.
Detailed description of the invention
Below in conjunction with accompanying drawing and following example, this utility model is described in further detail.
Embodiment 1
As shown in Figure 1, this utility model embodiment 1 provides a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen, including box body 1, the lid 2 hinged with described box body 1, lid 2 is hinged with box body 1, convenient fastening and unlatching, instrument cases 3 it are disposed with from top to bottom in described box body 1, appliance case 4 and drawing box 5, instrument cases 3 are for placing dropper required in experiment, test tube, reagent bottle, the instruments such as orifice plate, described appliance case 4 is used for placing magnetic means 6, described magnetic means 6 can be Magnet, the magnetic bodies such as magnetic grain, described appliance case 4 and described drawing box 5 are all slidably connected with described box body 1, conveniently open and take out magnetic means 6 or reagent bottle;Being provided with transverse slat 7 in described instrument cases 3, described transverse slat 7 is uniformly provided with pore 8, the vertical lower being positioned at described transverse slat 7 in described instrument cases 3 is provided with breakage-proof pad 9;During experiment, invisible spectro liquid can be placed in the pore 8 on transverse slat 7, and breakage-proof pad 9 is placed bottom Reagent Tube broken, and Reagent Tube has a protective effect.
It is provided with platform 10 in described drawing box 5, described platform 10 is provided with reagent rack 11, described reagent rack 11 is provided with fixing pad 12, and described fixing pad 12 has vestibule 13, places first reagent bottle the 14, second reagent bottle the 15, the 3rd reagent bottle 16 in described vestibule 13 respectively.Reagent rack 11 is used for placing reagent bottle, in use, it is possible to rotate reagent rack 11 thus reagent bottle in selective reagent frame 11;It is contained with balance activation buffer in described first reagent bottle 14, is contained with elution buffer A in described second reagent bottle 15, in described 3rd reagent bottle 16, is contained with recombinant protein A functionalization bacterial magnetic particles reagent.
Other reagent bottle can also be deposited in certain reagent rack 11, when experiment, select multiple reagent bottle as required.
Embodiment 2
As shown in Figure 2-5, a kind of test kit utilizing functionalization bacterial magnetic particles purification IgA albumen that this utility model embodiment 2 provides, this embodiment 2 further defines the structure of reagent rack 11 and instrument cases 3 on the basis of embodiment 1, improves the multifunctionality of test kit.
As in figure 2 it is shown, it should be noted that described reagent rack 11 includes base plate 17, case 18 and is fixed on the swingle 19 on described base plate 17, have through hole in the middle part of described case 18, described case 18 by described through hole cross-under on described swingle 19.Reagent rack 11 is used for placing reagent bottle, not only conveniently takes, puts reagent bottle, and reagent bottle is not easy to topple over and causes that liquid spills, and case 18 can be rotated by swingle 19, thus the reagent bottle that convenient selection is different.
As shown in Figure 3, in order to prevent adjacent reagent bottle from colliding with each other, the technical program further defines, it is divided into many sub-boxes 21 by baffle plate 20 in described case 18, being equipped with fixing pad 12 in multiple described sub-boxes 21, described first reagent bottle 14, described second reagent bottle 15 and described 3rd reagent bottle 16 are individually positioned in different described sub-boxes 21.Place one bottle of reagent bottle in every sub-box 21, and be provided with baffle plate 20 between adjacent sub-box 21, so adjacent reagent bottle is not easy collision, be convenient for carrying.
As shown in Figure 4, in order to facilitate experimental implementation, the technical program further defines, described instrument cases 3 medial wall is provided with every platform 22, described transverse slat 7 two ends fixed described on platform 22, being perpendicular to described transverse slat 7 in described instrument cases 3 and be provided with stringer board 23, described instrument cases 3 are divided into instrument by described stringer board 23 and hold district 24 and vessel hold district 25.Transverse slat 7 be overlapped on instrument cases 3 inwall on platform 22, it is possible to fixing connect, it is also possible to be designed as and removably connect, test tube is placed in the pore 8 of transverse slat 7, it is easy to detection, additionally, instrument cases 3 are divided into instrument by described stringer board 23 holds district 24 and vessel hold district 25, so that instrument separates with vessel, not only to prevent cross-contamination, and prevent glass drying oven from pulverizing, when producing, can hold in district 25 at vessel and prevent liner, it is prevented that vessel collide.
Additionally, preferably define in the technical program, on described transverse slat 7, corresponding described pore 8 is respectively provided with slot tag 26, is used for pasting reagent label in described slot tag 26.Reagent label in slot tag 26 can distinguish reagent very easily, it is prevented that mixes up reagent during experiment or mixes up test tube, it is ensured that the normal detection of experiment.
As it is shown in figure 5, appliance case 4 and drawing box 5 are easily opened in order to convenient, the technical program defines, outside described appliance case 4 and described drawing box 5, is equipped with handle 27.
This utility model is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under enlightenment of the present utility model; no matter but in its shape or structure, do any change; every have same or like with the application like technical scheme, all fall within protection domain of the present utility model.

Claims (6)

1. the test kit utilizing functionalization bacterial magnetic particles purification IgA albumen, including the lid (2) that box body (1) and described box body (1) are hinged, it is characterized in that, instrument cases (3), appliance case (4) and drawing box (5) it is disposed with from top to bottom in described box body (1), described appliance case (4) and described drawing box (5) are all slidably connected with described box body (1), are placed with magnetic means (6) in described appliance case (4);Transverse slat (7) it is provided with in described instrument cases (3), being uniformly provided with pore (8) on described transverse slat (7), the vertical lower being positioned at described transverse slat (7) in described instrument cases (3) is provided with breakage-proof pad (9);
Platform (10) it is provided with in described drawing box (5), described platform (10) is provided with reagent rack (11), described reagent rack (11) is provided with fixing pad (12), described fixing pad (12) has vestibule (13), in described vestibule (13), places the first reagent bottle (14), the second reagent bottle (15), the 3rd reagent bottle (16) respectively;It is contained with balance activation buffer in described first reagent bottle (14), it is contained with elution buffer A in described second reagent bottle (15), in described 3rd reagent bottle (16), is contained with recombinant protein A functionalization bacterial magnetic particles reagent.
2. utilize the test kit of functionalization bacterial magnetic particles purification IgA albumen as claimed in claim 1, it is characterized in that, described reagent rack (11) includes base plate (17), case (18) and the swingle (19) being fixed on described base plate (17), described case (18) middle part have through hole, described case (18) by described through hole cross-under on described swingle (19).
3. utilize the test kit of functionalization bacterial magnetic particles purification IgA albumen as claimed in claim 2, it is characterized in that, it is divided into many sub-boxes (21) by baffle plate (20) in described case (18), being equipped with fixing pad (12) in multiple described sub-boxes (21), described first reagent bottle (14), described second reagent bottle (15) and described 3rd reagent bottle (16) are individually positioned in different described sub-boxes (21).
4. utilize the test kit of functionalization bacterial magnetic particles purification IgA albumen as claimed in claim 1, it is characterized in that, described instrument cases (3) medial wall is provided with every platform (22), described transverse slat (7) two ends fixed described on platform (22), being perpendicular to described transverse slat (7) in described instrument cases (3) and be provided with stringer board (23), described instrument cases (3) hold district (24) by described stringer board (23) instrument of being divided into and vessel hold district (25).
5. utilize the test kit of functionalization bacterial magnetic particles purification IgA albumen as claimed in claim 4, it is characterized in that, the upper corresponding described pore (8) of described transverse slat (7) is respectively provided with slot tag (26), is used for pasting reagent label in described slot tag (26).
6. utilize the test kit of functionalization bacterial magnetic particles purification IgA albumen as claimed in claim 1, it is characterised in that described appliance case (4) and described drawing box (5) outside are equipped with handle (27).
CN201620138744.4U 2016-02-24 2016-02-24 Kit of utilization function ization bacterium magnetism granule purification igA albumen Expired - Fee Related CN205368202U (en)

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Application Number Priority Date Filing Date Title
CN201620138744.4U CN205368202U (en) 2016-02-24 2016-02-24 Kit of utilization function ization bacterium magnetism granule purification igA albumen

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Application Number Priority Date Filing Date Title
CN201620138744.4U CN205368202U (en) 2016-02-24 2016-02-24 Kit of utilization function ization bacterium magnetism granule purification igA albumen

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107368706A (en) * 2017-06-27 2017-11-21 中国水稻研究所 Sequencing data interpretation of result method and apparatus, sequencing library structure and sequence measurement

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107368706A (en) * 2017-06-27 2017-11-21 中国水稻研究所 Sequencing data interpretation of result method and apparatus, sequencing library structure and sequence measurement
WO2019001168A1 (en) * 2017-06-27 2019-01-03 中国水稻研究所 Sequencing data result analysis method and apparatus, and sequencing library construction and sequencing method

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CF01 Termination of patent right due to non-payment of annual fee
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Granted publication date: 20160706

Termination date: 20190224