CN205353100U - Blood platelet specificity self antibody test kit - Google Patents
Blood platelet specificity self antibody test kit Download PDFInfo
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- CN205353100U CN205353100U CN201521036627.9U CN201521036627U CN205353100U CN 205353100 U CN205353100 U CN 205353100U CN 201521036627 U CN201521036627 U CN 201521036627U CN 205353100 U CN205353100 U CN 205353100U
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Abstract
The utility model belongs to the technical field of the biological detection kit, concretely relates to blood platelet specificity self antibody test kit, including be square kit body and with body articulated lid, the holding has the reagent bottle to place the board in the body, the reagent bottle is placed the inboard and is equipped with reagent bottle that eight intervals set up and places the hole, downthehole holding is placed to every reagent bottle has a reagent bottle, the reagent bottle place the board below extraction formula be equipped with the reaction. The utility model discloses a blood platelet specificity self antibody test kit, FCM that can 5 kinds of platelet antibodies of synchronous analysis detects, and sensitivity is high, the specificity is good, the rate of missed diagnosis is low, and easy operation is conveniently.
Description
Technical field
This utility model belongs to biological detection reagent kit technical field, is specifically related to a kind of blood-platelet specific autoantibody detection kit.
Background technology
Hemorrhage is a kind of very common disease clinically, if can not get making a definite diagnosis in time treatment, then may result in having an intense pain, severe joint damage and fatal internal hemorrhage thus causing death, and the whole world only has the hemorrhage patient of 30% and obtains and make a definite diagnosis.Immune thrombocytopenia (ITP) is clinical most commonly seen hemorrhage, accounts for the 30% of hemorrhage sum, and American-European countries's annual morbidity is 5-10/10 ten thousand populations.Can occur at any age level, point acute and chronic two kinds, acute it is more common in child, chronic is more common in adult, common with less than 40 years old women, over-65s Late-onset rate is in rising trend.Clinical manifestation with the petechia of skin and ecchymosis, mucosa and visceral hemorrhage for feature.Being about one thousandth according to Xue You alliance of world survey data display ITP prevalence in 2010, China there are about 1,500,000 patients.ITP is caused platelet destruction to increase by body immune system dysfunction, thus causing the autoimmune hemolytic disease that number of platelets reduces, the platelet counts caused mainly due to the platelet destruction of blood-platelet specific autoantibody mediation reduces, therefore the diagnosis of ITP is had important clinical value by the detection of blood-platelet specific autoantibody.
Utility model content
The purpose of this utility model is to provide a kind of blood-platelet specific autoantibody detection kit, can the FCM detection of 5 kinds of platelet antibodies of Synchronization Analysis, highly sensitive, specificity good, rate of missed diagnosis is low, simple to operation.
For achieving the above object, this utility model be the technical scheme is that the test kit body including being square and the lid hinged with body, body contents is equipped with reagent bottle and places plate, reagent bottle is placed in plate and is provided with eight spaced reagent bottle putting holes, being equipped with a reagent bottle in each reagent bottle putting hole, the lower section of reagent bottle placement plate is removable is provided with reactive tank.
Preferably, eight reagent bottle include four big bottles and four bottles, have been sequentially placed platelet lysates liquid, platelet cleaning mixture and microsphere cleaning mixture in three in four big bottles；The sheep anti-human polyclonal antibody of FITC labelling, positive quality control product and microsphere it is sequentially placed in four bottles three.
Preferably, described microsphere includes the microsphere of the microsphere of anti-GP Ⅸ monoclonal antibody, the microsphere of anti-GP I b monoclonal antibody, the microsphere of anti-GP II b monoclonal antibody, the microsphere of anti-GP III a monoclonal antibody and anti-GMP-140 monoclonal antibody.
Preferably, described reagent bottle is placed and is provided with thermometer on plate.
Preferably, a lateral wall of described reactive tank is provided with handle.
Preferably, the bottom of described reactive tank be provided with two convex stupefied, body is provided with slide rail with corresponding position, convex stupefied position, described slide rail makes reactive tank and body be slidably connected with convex stupefied cooperation.
Preferably, described reagent bottle places the material of plate is sponge.
Preferably, described test kit body and lid adopt the mode of hasp to fasten.
Wherein, flow microsphere technology (cytometricbeadarray, CBA) new technique that flow cytometry grows up it is based on, it is with polystyrene microsphere for reaction interface, some small-molecule substances can be captured after utilizing monoclonal antibody specific to be coated, by detecting " amplification " effect of microsphere, these small-molecule substances are detected.In recent years, CBA is just more and more applied in the various liquid phases such as serum, blood plasma, cultivation cell conditioned medium, tear or aqueous humor the detection of soluble protein and cytokine.
The platelet destruction of blood-platelet specific autoantibody mediation is classical ITP pathogenesis.The ITP Platelet surface combination of about 50%～60% has IgG type autoantibody, one or more GP of recognizable platelet surface, including GP II b/ III a, GP I b/ Ⅸ, I a/ II a, IV, V etc..Wherein the platelet antibody of about 75% is all located at platelet membrane GP II b/ III a, GP I on b/ Ⅸ complex.Platelet antoantibody causes the mechanism that platelet destruction accelerates: autoantibody is combined in platelet, and the Fc section of antibody molecule combines with the Fc receptor (FcR) of macrophage on the one hand, causes that platelet is swallowed destruction；Antigen antibody complex can activate benefit C1q on the other hand, and each composition of complement system is activated in succession subsequently, and C3 pyrolysis product C3b is attached to platelet surface, and is combined with the C3b receptor of macrophage, also leads to platelet phagocytosis and destroys.Few patients's platelet antoantibody not only results in platelet destruction, it is also possible to cause platelet function abnormality.
Be coated anti-GP Ⅸ (monoclonal antibody SZ1), GP I b (monoclonal antibody SZ2), anti-GP II b (monoclonal antibody SZ22), anti-GP III a (monoclonal antibody SZ21), anti-GMP-140 (monoclonal antibody SZ51) monoclonal antibody microsphere can catch platelet-specific antigen antibody complex, after the sheep anti-human polyclonal antibody adding FITC labelling, flow cytometer detects average fluorescent strength value (Meanfluorescenceintensity, MFI) Platelet glycoprotein specific autoantibody situation can be reflected, thus realizing the diagnosis of ITP.
After adopting technique scheme, this utility model has the positive effect that:
Blood-platelet specific autoantibody detection kit (flow microsphere method) in this utility model specializes in detection ITP and GP I b, the GP Ⅸ on non-immunity patients with thrombocytopenia platelet membrane surface, GP II b, GP III a, GMP140 autoantibody, this product have employed flow microsphere technology, and the antibody starting material quality of use complies with the national standard requirements.Product performance index meets GB, highly sensitive, specificity good, rate of missed diagnosis is low, simple to operation, be better than at present in the platelet-associated antibody detection used, alternative MAIPA is as a kind of new practical experimental technique easy to spread.It is provided with eight reagent bottle putting holes, has two for standby hole, it is prevented that when the reagent that has is inadequate, use.
Accompanying drawing explanation
Fig. 1 is the axonometric chart of blood-platelet specific autoantibody detection kit of the present utility model.
Wherein: 1, body, 2, lid, 3, reagent bottle place plate, 4, reagent bottle putting hole, 5, reagent bottle, 6, reactive tank, 61, handle, 7, thermometer, 8, business card screens groove.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, this utility model is described in further detail.
As it is shown in figure 1, be blood-platelet specific autoantibody detection kit of the present utility model, including the test kit body 1 being square and lid 2, body 1 is hinged with lid 2, and the two adopts the mode of hasp to fasten.
Being equipped with reagent bottle in body 1 and place plate 3, reagent bottle is placed plate 3 and is made for flexible sponge material.Reagent bottle is placed in plate 3 and is provided with eight spaced reagent bottle putting holes 4, is equipped with a reagent bottle 5 in each reagent bottle putting hole 4.Eight reagent bottle 5 include four big bottles and four bottles, have been sequentially placed platelet lysates liquid, platelet cleaning mixture and microsphere cleaning mixture in three in four big bottles；The sheep anti-human polyclonal antibody of FITC labelling, positive quality control product and microsphere it is sequentially placed in four bottles three.Described microsphere includes the microsphere of the microsphere of anti-GP Ⅸ monoclonal antibody, the microsphere of anti-GP I b monoclonal antibody, the microsphere of anti-GP II b monoclonal antibody, the microsphere of anti-GP III a monoclonal antibody and anti-GMP-140 monoclonal antibody.Reagent bottle is placed and is also equipped with thermometer 5 on plate 3.
Reagent bottle place plate 3 be connected with reactive tank 6, reactive tank 6 is drawing and pulling type.The bottom of reactive tank 6 be provided with two parallel convex stupefied, body 1 is provided with slide rail, and slide rail is corresponding with convex stupefied position, stupefied makes reactive tank 6 opposing body 1 to slide with coordinating of slide rail by convex, and reactive tank 6 will not be crooked in draw process, impact detection.Reactive tank 6 one is provided with handle 61 on lateral wall, for the ease of the pull to reactive tank 6.
One lateral wall of body 1 is provided with business card screens groove 8, business card screens groove 8 be disposed to be easy to be inserted into the business card of information-recording, it is simple to record testing result information.
Preparation method is as follows:
1. mainly comprise component analysis:
Table 1 component list
2. the method for inspection
(1) prepared by platelet: collect specimen 200g is centrifuged 5min, separates platelet rich plasma (PRP), takes PRP, the centrifugal 2min of 3000 × g again, blood plasma-20 DEG C preservation, platelet 800ul platelet cleaning mixture washs 3 times ,-20 DEG C of preservations (holding time: two weeks)；
(2) after adding the concussion mixing of 110ul lysate in platelet, room temperature shaker cracking 30min, 3000g are centrifuged 20min；
(3) taking supernatant 100ul to another EP pipe, add the microsphere mixed liquor 50ul being coated 5 kinds of different monoclonal antibodies, concussion mixing, room temperature lucifuge hatches 1h；
(4) adding 600ul microsphere cleaning mixture, 1500g is centrifuged 20min, gentle aspiration supernatant, and 100ul debris is about stayed in bottom；
(5) GAH10ul of FITC labelling, concussion mixing are added.Room temperature lucifuge hatches 30min；
(6) adding 600ul microsphere cleaning mixture, 1500g is centrifuged 20min, gentle aspiration supernatant, and 100ul debris is about stayed in bottom；
(7) 500ul microsphere cleaning mixture, upper machine testing are added；
(8) at flow cytometer (CytomicsFC-500, Beckman-Coulter, USA) scattered coloured light logarithm (SSC-LOG) scatterplot of scattered coloured light logarithm (the FSC-LOG)/lateral angle of forward angle marks detection microsphere door (A), analyze (it is the most clear that excitation wavelength selects 488nm or 633nm to make between each peak of microsphere) rectangular histogram of microsphere in A door, regulate FL-5 passage and five fluorescence peaks uniformly occur, count 5,000 microspheres, analyze FITC average fluorescent strength value (Meanfluorescenceintensity, MFI) of each fluorescence peak.
It will be apparent to those skilled in the art that can technical scheme as described above and design, make other various corresponding changes and deformation, and all these change and deformation all should belong within the protection domain of this utility model requirement.
Claims (8)
1. a blood-platelet specific autoantibody detection kit, it is characterized in that: include the test kit body (1) that is square and the lid (2) hinged with body (1), it is equipped with reagent bottle in body (1) and places plate (3), reagent bottle is placed in plate (3) and is provided with eight spaced reagent bottle putting holes (4), being equipped with a reagent bottle (5) in each reagent bottle putting hole (4), the lower section of reagent bottle placement plate (3) is removable is provided with reactive tank (6).
2. blood-platelet specific autoantibody detection kit according to claim 1, it is characterized in that: eight reagent bottle (5) include four big bottles and four bottles, have been sequentially placed platelet lysates liquid, platelet cleaning mixture and microsphere cleaning mixture in three in four big bottles；The sheep anti-human polyclonal antibody of FITC labelling, positive quality control product and microsphere it is sequentially placed in four bottles three.
3. blood-platelet specific autoantibody detection kit according to claim 2, it is characterised in that: described microsphere includes the microsphere of the microsphere of anti-GP Ⅸ monoclonal antibody, the microsphere of anti-GP I b monoclonal antibody, the microsphere of anti-GP II b monoclonal antibody, the microsphere of anti-GP III a monoclonal antibody and anti-GMP-140 monoclonal antibody.
4. blood-platelet specific autoantibody detection kit according to claim 1, it is characterised in that: reagent bottle is placed and is provided with thermometer (7) on plate (3).
5. blood-platelet specific autoantibody detection kit according to claim 1, it is characterised in that: a lateral wall of reactive tank (6) is provided with handle (61).
6. blood-platelet specific autoantibody detection kit according to claim 5, it is characterized in that: the bottom of reactive tank (6) be provided with two convex stupefied, body (1) is upper is provided with slide rail with corresponding position, convex stupefied position, and described slide rail makes reactive tank (6) and body (1) be slidably connected with convex stupefied cooperation.
7. blood-platelet specific autoantibody detection kit according to claim 6, it is characterised in that: it is sponge that reagent bottle places the material of plate (3).
8. blood-platelet specific autoantibody detection kit according to claim 1, it is characterised in that: test kit body (1) and lid (2) adopt the mode of hasp to fasten.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521036627.9U CN205353100U (en) | 2015-12-14 | 2015-12-14 | Blood platelet specificity self antibody test kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521036627.9U CN205353100U (en) | 2015-12-14 | 2015-12-14 | Blood platelet specificity self antibody test kit |
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| CN205353100U true CN205353100U (en) | 2016-06-29 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106236779A (en) * | 2016-08-22 | 2016-12-21 | 孔五 | A kind of preparation method of Cord blood platelet rich plasma PRP |
| CN107727843A (en) * | 2017-10-12 | 2018-02-23 | 苏州大学 | Detect reagent of antiplatelet surface receptor specificity autoantibody and preparation method and application |
| CN108715833A (en) * | 2018-06-01 | 2018-10-30 | 天晴干细胞股份有限公司 | A kind of method for preparing microsphere of load platelet lysates liquid |
| CN116539872A (en) * | 2023-04-25 | 2023-08-04 | 上海润普生物技术有限公司 | Fluorescent immunochromatography detection kit for quantitative platelet antibody and preparation method thereof |
-
2015
- 2015-12-14 CN CN201521036627.9U patent/CN205353100U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106236779A (en) * | 2016-08-22 | 2016-12-21 | 孔五 | A kind of preparation method of Cord blood platelet rich plasma PRP |
| CN107727843A (en) * | 2017-10-12 | 2018-02-23 | 苏州大学 | Detect reagent of antiplatelet surface receptor specificity autoantibody and preparation method and application |
| CN108715833A (en) * | 2018-06-01 | 2018-10-30 | 天晴干细胞股份有限公司 | A kind of method for preparing microsphere of load platelet lysates liquid |
| CN108715833B (en) * | 2018-06-01 | 2021-09-14 | 天晴干细胞股份有限公司 | Preparation method of microsphere loaded with platelet lysate |
| CN116539872A (en) * | 2023-04-25 | 2023-08-04 | 上海润普生物技术有限公司 | Fluorescent immunochromatography detection kit for quantitative platelet antibody and preparation method thereof |
| CN116539872B (en) * | 2023-04-25 | 2024-02-09 | 上海润普生物技术有限公司 | Fluorescent immunochromatography detection kit for quantitative platelet antibody and preparation method thereof |
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Address after: No. 18, Jincheng Road, economic development zone, Wujin District, Changzhou City, Jiangsu Province Patentee after: Changzhou Fucheng biomedical Co.,Ltd. Patentee after: Suzhou University Address before: No. 18, Jincheng Road, economic development zone, Wujin District, Changzhou City, Jiangsu Province Patentee before: CHANGZHOU FULONG BIOLOGICAL Co.,Ltd. Patentee before: Suzhou University |
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| CP03 | Change of name, title or address |
Address after: 215000 room 304, building 1, Suzhou nano Health Industrial Park, No. 333 Xingpu Road, Suzhou Industrial Park, Jiangsu Province Patentee after: Suzhou Yuande Weikang biomedical Co.,Ltd. Patentee after: Soochow University Address before: No. 18, Jincheng Road, economic development zone, Wujin District, Changzhou City, Jiangsu Province Patentee before: Changzhou Fucheng biomedical Co.,Ltd. Patentee before: Soochow University |
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| CP03 | Change of name, title or address |