CN205317783U - Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card - Google Patents
Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card Download PDFInfo
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- CN205317783U CN205317783U CN201620080525.5U CN201620080525U CN205317783U CN 205317783 U CN205317783 U CN 205317783U CN 201620080525 U CN201620080525 U CN 201620080525U CN 205317783 U CN205317783 U CN 205317783U
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- test paper
- platelet
- lase
- fluorescent quantitation
- activating factor
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Abstract
The utility model provides a relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card, test paper includes the bottom plate, has set gradually that sample pads, fluorescein combine pad, nitrocellulose membranes and the layer that absorbs water along the bottom plate length direction on, the edge last relevant 2 antibody of phospholipase A of peridium lipoprotein that lean on respectively of nitrocellulose membranes length direction constitute one bellied detection line with leaning on the anti mouse polyclonal antibody of peridium to constitute bellied matter accuse line, detecting the card and including last casing and lower casing, go up the casing and form a casing with the lower mutual lock of casing, the relevant phospholipase A 2 fluorescent quantitation test paper of above -mentioned lipoprotein has been placed to casing inside, goes up housing face counter sample pad department and has offered the application of sample hole, and corresponding nitrocellulose membranes department has offered detection window. The utility model discloses test paper can survey object content in serum, the plasma / whole blood, and it is more simple and convenient to compare prior art operation, and testing result good reproducibility, stability are strong.
Description
Technical field
This utility model belongs to lipoprotein associated phospholipase detection technique field, is specifically related to a kind of platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper and detection card.
Background technology
Platelet-activating factor acetylhydro-lase (Lp-PLA2) relative molecular mass is about 45400, is a member in phospholipase A2 superfamily, containing 441 amino acid residues. Human plasma Lp-PLA2 is synthesized and secretion by macrophage, lymphocyte and mastocyte, has the effect of pro-atherogenic, can be regulated by inflammatory mediator, is a kind of new inflammatory reaction mark, all plays effect in several Main Stage of AS. Lp-PLA2 is mainly produced by macrophage and lymphocyte, in early days about in the research of Lp-PLA2 and AS, because Lp-PLA2 can be hydrolyzed pro-inflammatory cytokine such as platelet activating factor (plateletactivatingfactor, and the relevant oxidized phospholipids of structure PAF), therefore once it was considered to suppress inflammatory reaction, even can suppress atherosclerotic formation, therefore it is also referred to as platelet-activating factor acetylhydrolase (plateletactivatingfactoracetylhydrolase, PAF-AH). But research in recent years has proven to effect bigger for Lp-PLA2 is in that to promote generation and the development of AS. Lp-PLA2 is by being hydrolyzed the oxidation lecithin on the LDL on endarterium, thus generating LYSOLECITHIN SUNLECITHIN A (Lysophosphatidylcholine, and free oxidation of fat acid (OxidizedFatteyAcid Lyso-PC), ox-FA), the latter two are pro-inflammatory mediators, the generation of adhesion factor and cytokine can be stimulated, thus causing that mononuclear cell is assembled to inner membrance by tube chamber.Mononuclear cell derives as macrophage after inner membrance is assembled, and macrophage phagocytic oxLDL becomes the foam cell of apoptosis, and the macrophage of these activation and foam cell can produce more Lp-PLA2 and return to circulation. The foam cell of apoptosis is gathered into atherosclerotic plaques, the speckle energy release cells factor and protease, the smooth muscle cell of degradation of fibers cap and collagen stroma, make speckle become fragile, break, thus causing generation and the development of AS, cause the generation of thrombosis and cardiovascular event.
The reagent being currently used for detection Lp-PLA2 is mainly the product of enzyme linked immunosorbent assay principle, uses the principle that antigen and antibody specific combines to detect, and detection product exists complex operation, the shortcomings such as data redundancy is poor, expensive, poor stability.
Utility model content
For prior art above shortcomings, technical problem to be solved in the utility model is: how to provide a kind of platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper and detection card, for measuring the content of the platelet-activating factor acetylhydro-lase in serum, blood plasma/whole blood, auxiliary diagnosis myocardial damage, and have the advantages that operation is rapid and simple, detect data accurate stable.
In order to solve above-mentioned technical problem, this utility model adopts the following technical scheme that a kind of platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper, including base plate, it is disposed with sample pad along described floor length direction, fluorescein pad, nitrocellulose filter and water accepting layer, described sample pad is overlapped on fluorescein pad near one end of described fluorescein pad, described fluorescein pad is overlapped on nitrocellulose filter near one end of described nitrocellulose filter, described water accepting layer is overlapped on nitrocellulose filter near one end of described nitrocellulose filter, it is characterized in that, along described nitrocellulose filter length direction respectively by be coated platelet-activating factor acetylhydro-lase antibody constitute together protruding detection line with lean on be coated against murine polyclonal antibody constitute together with the nature controlling line of projection. adopt the mode of this overlap joint, it is possible to be easy to sample upwards chromatography. during use, detected sample is dropped in sample pad, flowed through fluorescein pad by chromatography effect detected sample by sample pad, arrive nitrocellulose filter, and continue to continue flow chromatographic at nitrocellulose filter, arrive detection line and nature controlling line, if containing platelet-activating factor acetylhydro-lase in sample, then this target detection thing will react by coated material on detection line, detection line colour developing, if without there being object to be detected in sample, then detection line will not develop the color, regardless of whether whether contain object to be detected in sample, sample all can react with the coated material of nature controlling line, and nature controlling line develops the color, and nature controlling line is used for verifying that reagent paper can normally carry out chromatography.
As optimization, described detection line and nature controlling line be arranged in parallel. So, it is to avoid detection line and nature controlling line intersect, and then avoid detection line and nature controlling line is coated the generation of thing phase mixing phenomena, make the testing result more accurate.
As optimization, described detection line is identical with the width of described nitrocellulose filter with the length of nature controlling line. So, convenient testing staff observes testing result.
As optimization, the distance between described detection line and nature controlling line is 5 ~ 15mm. So making to keep between detection line and nature controlling line suitable distance, it is to avoid detection line or after nature controlling line colour developing, diffusing phenomenon occurs, making testing staff judge sample by accident is false positive, testing result is relatively reliable.
As further optimization, the material of described base plate is polrvinyl chloride.The base plate using this material has good water resistance, will not be infiltrated in detection process, and then testing result will not be brought adverse effect.
As further optimization, described detection line is positioned close to the side of fluorescein pad. So, testing sample chromatography to nitrocellulose filter after, first flow through detection line, then through nature controlling line, decrease sample first with nature controlling line be coated after thing reacts to be likely to the detection impact brought to detected sample, make testing result relatively reliable.
A kind of platelet-activating factor acetylhydro-lase fluorescent quantitation detection card, including upper shell and lower house, described upper shell and lower house fasten one housing of formation mutually, it is characterized in that, described enclosure interior is placed with above-mentioned platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper, corresponding described sample pad place, described upper shell surface offers well, and corresponding described nitrocellulose filter place offers detection window. Above-mentioned Test paper and housing are assembled and makes detection card, Test paper is had certain protective effect, decreases Test paper generation of contaminated phenomenon during shelf, and convenient testing staff grips, and conveniently detects.
As optimization, described lower house inner surface is provided with support, and described platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper binds on the bracket. As such, it is possible to make Test paper to be subjected to displacement in the housing, well and detection window deviation sample pad and celluloid film location will not be made, and then do not result in application of sample and read result mistake.
As further optimization, the material of described upper shell and lower house is polyethylene, polypropylene, polrvinyl chloride, polystyrene or acrylonitrile-butadiene-styrene copolymerized compound. Select such material, it is possible to the water resistance making detection card is good, and more wear-resisting resistance to fall.
As another optimization, the aperture of described well is 0.5 ~ 0.8mm. Select such aperture, both avoided the problem that the too small application of sample of well is inconvenient, turn avoid the excessive problem needing sample pad volume excessive of well.
Compared to existing technology, this utility model has the advantages that
1, this utility model platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper adopts immunochromatographic method, the content of the platelet-activating factor acetylhydro-lase in serum, blood plasma/whole blood can be measured, auxiliary diagnosis myocardial damage, compare the enzyme linked immunosorbent assay of prior art, operate simpler convenience, testing result reads convenient, and testing result is reproducible, stability is strong.
2, this utility model detection card compact, it is convenient for carrying, testing staff is facilitated to carry out in situ detection, and without using special detectable and detecting instrument during detection, only need that detection sample drop is added to well and can be carried out detection, namely testing result can read in 3 ~ 5min, more solves the detection time.
3, the structure of this utility model Test paper and detection card is prone to carry out high-volume production and processing continuously, has good market prospect.
Accompanying drawing explanation
Fig. 1 is this utility model Test paper structural representation;
Fig. 2 is this utility model detection card sectional view;
Fig. 3 is this utility model detection card top view;
Accompanying drawing labelling: 1-sample pad, 2-fluorescein pad, 3-detects line, 4-nitrocellulose filter, 5-nature controlling line, 6-water accepting layer, 7-base plate, 8-Test paper, 9-housing, 10-support, 11-well, 12-detection window.
Detailed description of the invention
Below in conjunction with specific embodiments and the drawings, this utility model is described in further detail.
When being embodied as, a kind of platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper, as shown in Figure 1, including base plate 7, it is disposed with sample pad 1 along described base plate 7 length direction, fluorescein pad 2, nitrocellulose filter 4 and water accepting layer 6, described sample pad 1 is overlapped on fluorescein pad 2 near one end of described fluorescein pad 2, described fluorescein pad 2 is overlapped on nitrocellulose filter 4 near one end of described nitrocellulose filter 4, described water accepting layer 6 is overlapped on nitrocellulose filter 4 near one end of described nitrocellulose filter 4, along described nitrocellulose filter 4 length direction respectively by be coated platelet-activating factor acetylhydro-lase antibody constitute together protruding detection line 3 with lean on be coated against murine polyclonal antibody constitute together with the nature controlling line 5 of projection. adopt the mode of this overlap joint, it is possible to be easy to sample upwards chromatography. during use, detected sample is dropped in sample pad, flowed through fluorescein pad by chromatography effect detected sample by sample pad, arrive nitrocellulose filter, and continue to continue flow chromatographic at nitrocellulose filter, arrive detection line and nature controlling line, if containing platelet-activating factor acetylhydro-lase in sample, then this target detection thing will react by coated material on detection line, detection line colour developing, if without there being object to be detected in sample, then detection line will not develop the color, regardless of whether whether contain object to be detected in sample, sample all can react with the coated material of nature controlling line, and nature controlling line develops the color, and nature controlling line is used for verifying that reagent paper can normally carry out chromatography.
Detect line 3 described in above-mentioned Test paper and nature controlling line 5 can parallel to each other be arranged. So, it is to avoid detection line and nature controlling line intersect, and then avoid detection line and nature controlling line is coated the generation of thing phase mixing phenomena, make the testing result more accurate.
Detecting line 3 described in above-mentioned Test paper can be identical with the width of described nitrocellulose filter 4 with the length of nature controlling line 5. So, convenient testing staff observes testing result.
The distance detected between line 3 and nature controlling line 5 described in above-mentioned Test paper can be 5 ~ 15mm, so make to keep between detection line and nature controlling line suitable distance, diffusing phenomenon is there is after avoiding detection line or nature controlling line colour developing, making testing staff judge sample by accident is false positive, and testing result is relatively reliable.
The material of base plate 7 described in above-mentioned Test paper can be polrvinyl chloride, uses the base plate of this material to have good water resistance, will not be infiltrated, and then testing result will not be brought adverse effect in detection process.
Detect line 3 described in above-mentioned Test paper and be positioned close to the side of fluorescein pad 2. So, testing sample chromatography to nitrocellulose filter after, first flow through detection line, then through nature controlling line, decrease sample first with nature controlling line be coated after thing reacts to be likely to the detection impact brought to detected sample, make testing result relatively reliable.
When being embodied as, a kind of platelet-activating factor acetylhydro-lase fluorescent quantitation detection card, as shown in Figure 2, including upper shell and lower house, described upper shell and lower house fasten one housing 9 of formation mutually, and described enclosure interior is placed with above-mentioned platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper 8, as shown in Figure 3, corresponding described sample pad 1 place, described upper shell surface offers well 11, and corresponding described nitrocellulose filter 4 place offers detection window 12.Above-mentioned Test paper and housing are assembled and makes detection card, Test paper is had certain protective effect, decreases Test paper generation of contaminated phenomenon during shelf, and convenient testing staff grips, and conveniently detects.
As in figure 2 it is shown, the described lower house inner surface of above-mentioned detection card can be provided with support 10, described platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper 8 binds on described support 10. As such, it is possible to make Test paper to be subjected to displacement in the housing, well and detection window deviation sample pad and celluloid film location will not be made, and then do not result in application of sample and read result mistake.
The material of upper shell and lower house described in above-mentioned detection card can be polyethylene, polypropylene, polrvinyl chloride, polystyrene or acrylonitrile-butadiene-styrene copolymerized compound. Select such material, it is possible to the water resistance making detection card is good, and more wear-resisting resistance to fall.
The aperture of well 11 described in above-mentioned detection card can be 0.5 ~ 0.8mm. Select such aperture, both avoided the problem that the too small application of sample of well is inconvenient, turn avoid the excessive problem needing sample pad volume excessive of well.
In above-mentioned detection card, described upper shell surface can be provided with to cover the sealing member of described well 11 and detection window 12, described sealing member can be plate pushing structure, the side of described lower house is provided with chute, and described push pedal can move to the other end from the one end in shell length direction along described chute. This way it is possible to avoid detection is stuck in when preservation or transport contaminated.
In above-mentioned detection card, corresponding described water accepting layer 6 place, described upper shell surface can be provided with passage, breathes freely after the water suction of such convenient absorbent layer, it also avoid detection card accumulated inside steam during preserving.
Be worth propose be, above example is only in order to illustrate the technical solution of the utility model and unrestricted, although this utility model being described in detail with reference to preferred embodiment, it will be understood by those within the art that, the technical solution of the utility model can be modified or equivalent replacement, without deviating from objective and the scope of technical solutions of the utility model, it all should be encompassed in the middle of right of the present utility model.
Claims (10)
1. a platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper, including base plate, it is disposed with sample pad along described floor length direction, fluorescein pad, nitrocellulose filter and water accepting layer, described sample pad is overlapped on fluorescein pad near one end of described fluorescein pad, described fluorescein pad is overlapped on nitrocellulose filter near one end of described nitrocellulose filter, described water accepting layer is overlapped on nitrocellulose filter near one end of described nitrocellulose filter, it is characterized in that, along described nitrocellulose filter length direction respectively by be coated platelet-activating factor acetylhydro-lase antibody constitute together protruding detection line with lean on be coated against murine polyclonal antibody constitute together with the nature controlling line of projection.
2. platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper according to claim 1, it is characterised in that described detection line and nature controlling line be arranged in parallel.
3. platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper according to claim 1, it is characterised in that described detection line is identical with the width of described nitrocellulose filter with the length of nature controlling line.
4. platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper according to claim 1, it is characterised in that the distance between described detection line and nature controlling line is 5 ~ 15mm.
5. platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper according to claim 1, it is characterised in that the material of described base plate is polrvinyl chloride.
6. platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper according to claim 1, it is characterised in that described detection line is positioned close to the side of fluorescein pad.
7. a platelet-activating factor acetylhydro-lase fluorescent quantitation detection card, including upper shell and lower house, described upper shell and lower house fasten one housing of formation mutually, it is characterized in that, described enclosure interior is placed with the arbitrary described platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper of claim 1 ~ 6, corresponding described sample pad place, described upper shell surface offers well, and corresponding described nitrocellulose filter place offers detection window.
8. the detection of platelet-activating factor acetylhydro-lase fluorescent quantitation blocks according to claim 7, it is characterised in that be provided with support on described lower house inner surface, and described platelet-activating factor acetylhydro-lase fluorescent quantitation Test paper binds on the bracket.
9. the detection of platelet-activating factor acetylhydro-lase fluorescent quantitation blocks according to claim 7, it is characterised in that the material of described upper shell and lower house isPolyethylene、Polypropylene, polrvinyl chloride,PolystyreneOr acrylonitrile-Butadiene-styrene copolymerized compound.
10. the detection of platelet-activating factor acetylhydro-lase fluorescent quantitation blocks according to claim 7, it is characterised in that the aperture of described well is 0.5 ~ 0.8mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620080525.5U CN205317783U (en) | 2016-01-27 | 2016-01-27 | Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620080525.5U CN205317783U (en) | 2016-01-27 | 2016-01-27 | Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card |
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| CN205317783U true CN205317783U (en) | 2016-06-15 |
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| CN201620080525.5U Expired - Fee Related CN205317783U (en) | 2016-01-27 | 2016-01-27 | Relevant phospholipase A 2 fluorescent quantitation test paper of lipoprotein and detection card |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645730A (en) * | 2016-12-15 | 2017-05-10 | 威海纽普生物技术有限公司 | Kit for determining lipoprotein-associated phospholipase A2 and manufacturing method |
| CN106771124A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card |
-
2016
- 2016-01-27 CN CN201620080525.5U patent/CN205317783U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771124A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of platelet-activating factor acetylhydro-lase fluorogenic quantitative detection test paper and detection card |
| CN106645730A (en) * | 2016-12-15 | 2017-05-10 | 威海纽普生物技术有限公司 | Kit for determining lipoprotein-associated phospholipase A2 and manufacturing method |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160615 Termination date: 20170127 |
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| CF01 | Termination of patent right due to non-payment of annual fee |