CN205278512U

CN205278512U - A little valve of high leakproofness for PCR reaction

Info

Publication number: CN205278512U
Application number: CN201520927684.XU
Authority: CN
Inventors: 刘佐雄
Original assignee: Beijing Eternal Life Technology Co Ltd
Current assignee: Beijing Eternal Life Technology Co Ltd
Priority date: 2015-11-20
Filing date: 2015-11-20
Publication date: 2016-06-01
Anticipated expiration: 2025-11-20

Abstract

The utility model provides a little valve of high leakproofness for PCR reaction, little valve includes motor driver, case and cover plate, formation fluid passage and sealed is connected with the PCR chip to little valve the reaction chamber of PCR chip, motor driver includes support, motor and ball, the upper end of case with ball connects, the lower extreme of case passes the cover plate and is connected with the reaction chamber of PCR chip. The beneficial effects are that, has simple structure, advantage that the leakproofness is high.

Description

A kind of micro-valve of high leakproofness for PCR reaction

Technical field

The utility model relates to PCR instrument device equipment technical field, specifically relates to a kind of micro-valve of high leakproofness for PCR reaction.

Background technology

Micro-valve is the crucial execution unit in PCR micro-fluidic chip, for the duplication of DNA provides closed environment in polymerase chain reaction, is the efficient quick response prerequisite of PCR.

Current micro-valve of a great variety, their each have their own relative merits. Wherein, piezoelectric micromotor valve is highly sensitive, and rapidly, dead volume is little, but stroke is short, and stopping property is poor in reaction. The micro-valve arrangement more complicated of magnetic, the micro-valve arrangement of shape memory alloy is simple, compact, has higher output rating; The micro-valve of phase transformation widely uses in disposable biological chip, but it is slow to open the time; The micro-valve arrangement of kapillary is simply easy to integrated, but poor sealing performance.

Practical novel content

The purpose of this utility model is to provide a kind of micro-valve of high leakproofness for PCR reaction.

The utility model realizes the technical scheme of goal of the invention employing, a kind of micro-valve of high leakproofness for PCR reaction, described micro-valve comprises motor drive unit, spool and lid sheet, described micro-valve and pcr chip are connected to form fluid channel and seal the reaction chamber of described pcr chip, described motor drive unit comprises support, motor and ball-screw, the upper end of described spool is connected with described ball-screw, and the lower end of described spool is connected through lid sheet with the reaction chamber of pcr chip.

Specifically, described spool is made up of valve head and valve rod, and on described lid sheet, corresponding stem position is provided with through hole, and described valve rod is through described through hole.

Preferably, the reaction chamber of pcr chip is circular.

The beneficial effects of the utility model are, it is simple that micro-valve of the present utility model has structure, the advantage that stopping property is high.

Accompanying drawing explanation

Fig. 1, the structure iron of embodiment motor drive unit.

Fig. 2, the connection structure iron of embodiment spool and pcr chip.

Fig. 3, the structure iron of embodiment pcr chip reaction chamber.

Embodiment

Below in conjunction with accompanying drawing, the utility model is described in detail.

A kind of micro-valve of high leakproofness for PCR reaction, comprise motor drive unit, spool and lid sheet, the structure iron of motor drive unit is referring to accompanying drawing 1, the connection structure iron of spool and pcr chip is referring to accompanying drawing 2, micro-valve and pcr chip are connected to form fluid channel and seal the reaction chamber of pcr chip, motor drive unit comprises support 1, motor 2 and ball-screw 3, spool is made up of valve head 4 and valve rod 5, the upper end of spool is connected with ball-screw 3, on lid sheet 6, corresponding valve rod 5 position is provided with through hole, and valve rod 5 is connected through lid sheet 6 through hole with the reaction chamber 8 of pcr chip 7.

In the present embodiment, the reaction chamber 8 of pcr chip 7 is designed to circular configuration, its structure iron is referring to accompanying drawing 3, circular configuration is to the introducing of reagent and flows out favourable, reagent can be full of reaction chamber very soon when flowing into reaction chamber along fluid channel, during outflow, under the effect of pump, the solution overwhelming majority can flow out, and dead volume is little.

Micro-valve arrangement of the present utility model mainly comprises three parts, is respectively upper motor drive unit, and middle spool, bottom is the pcr chip being bonded together by pressure sintering. What spool was vertical is put in microfluidic channel and the top of lid sheet, and valve rod aligns with the through hole of lid sheet. The consistent size of lid sheet and micro-fluid chip, has two through holes of diameter at a distance of 7mm above. The typical sizes of microfluidic channel is 0.4mm*07mm. The volume of PCR reaction chamber is 30ul, and its structure is cylindrical. Implementing mode as other, micro-fluid chip also machined valve seat construction, it is round boss that diameter is 1mm.

The principle of work of the micro-valve of the utility model is simple, and spool is positioned at the surface of whole chip, and valve rod aligns with the through hole of lid sheet. It is normally open valve, and when being in off working state, spool is positioned at above lid sheet, and valve is opened, and fluid can unrestricted flow. Opening motor, spool is lower under the effect of motor torsional moment to be moved, and flow velocity reduces gradually along with the lower shifting of spool, and when spool contacts completely with valve seat, valve cuts out, and fluid stops flowing, and whole PCR reaction solution is sealed in reaction chamber. Then disable motor, temperature controlling system is started working, when PCR after completion of the reaction, open motor, motor reversal, drive and spool moves, valve is opened, and reacts complete solution under the effect of pump, enters fluoroscopic examination district and carry out fluoroscopic examination.

Last it is noted that above embodiment only in order to the utility model is described and and technical scheme described by unrestricted the utility model; Although therefore the utility model has been described in detail with reference to each above-mentioned embodiment by this specification sheets, but it will be understood by one skilled in the art that and still the utility model can be modified or equivalent replacement; And all do not depart from technical scheme and the improvement thereof of spirit and scope of the present utility model, it all should be encompassed in right of the present utility model.

Claims

1. the micro-valve of high leakproofness for PCR reaction, it is characterized in that: described micro-valve comprises motor drive unit, spool and lid sheet, described micro-valve and pcr chip are connected to form fluid channel and seal the reaction chamber of described pcr chip, described motor drive unit comprises support, motor and ball-screw, the upper end of described spool is connected with described ball-screw, and the lower end of described spool is connected through lid sheet with the reaction chamber of pcr chip.

2. the micro-valve of high leakproofness for PCR reaction according to claim 1, it is characterised in that: described spool is made up of valve head and valve rod, and on described lid sheet, corresponding stem position is provided with through hole, and described valve rod is through described through hole.

3. the micro-valve of high leakproofness for PCR reaction according to claim 1, it is characterised in that: the reaction chamber of pcr chip is circular.