CN205275570U - Automatic enzymolysis isolated cell device - Google Patents
Automatic enzymolysis isolated cell device Download PDFInfo
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- CN205275570U CN205275570U CN201521026285.2U CN201521026285U CN205275570U CN 205275570 U CN205275570 U CN 205275570U CN 201521026285 U CN201521026285 U CN 201521026285U CN 205275570 U CN205275570 U CN 205275570U
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Abstract
The utility model discloses an automatic enzymolysis isolated cell device, it includes hollow box, and the box top is equipped with a plurality of to the sunken recess in box inside, and every recess interpolation is equipped with one and places the cup, places bottom of cup portion and is equipped with the filter membrane, places glass upper portion and is equipped with the screw thread, the symmetry is equipped with a pair of electric lift rod on the box top surface, is equipped with closed frame between two electric lift rod. The utility model has the advantages of, accurate control enzymatic hydrolysis system temperature is guaranteed the enzymolysis reaction and is gone on under suitable temperature, improves the reaction efficiency of enzyme, electric lift rod passes through the controller interoperation with the time -recorder, when the enzymolysis reaction finishes, in time places the cup with all and raises, avoids cell activity to reduce because of the hydrolysis time overlength, the labor intensity of artifical participation degree among the enzymolysis process on the one hand can be artifical is reduced, improve work efficiency, on the other hand avoids the manual operation to carry over enzymatic hydrolysis system reagent out of and places the outer test for contamination environment of cup.
Description
Technical field:
The utility model relates to a kind of tripping device, in particular to the automatic enzymolysis isolated cell device of one.
Background technology:
Animal embryo implantation technique is in the process implemented, need body early embryo is separated into individual cells, again individual cells is implanted in enucleation oocyte, the mode that embryo is separated into individual cells adopts mechanical separation to be separated with enzymolysis usually, wherein, enzymolysis separation is because having mild condition, separation efficiency height, be separated the high advantage of rear cytoactive, and is widely adopted. But in the process of enzymolysis isolated cell, need enzyme digestion reaction system temperature and enzymolysis time to be controlled, enzyme digestion reaction system temperature is too high or too low all can cause enzymic activity to reduce, and enzymolysis time is crossed length and cytoactive can be caused to reduce or cell injury, affects the effect of later stage embryo transfer.
Practical novel content:
The purpose of this utility model is to provide a kind of enzymolysis isolated cell device reducing hand labor intensity, improving enzymolysis isolated cell efficiency, avoid testing circumstance to pollute.
The utility model is implemented by following technical scheme: it comprises the casing of hollow, described casing top is provided with some the grooves caved in described box house, being inserted with one in groove described in each and place cup, be provided with filter membrane bottom described placement cup, described placement cup top is provided with screw thread; Described box top is arranged with one pair of electric up-down bar, described in two, between electric up-down bar, is provided with closure frame, the envelope lid that on described closure frame, corresponding described placement cup is fixed with described placement cup spiral shell connects; It is filled with heat-conducting medium in described casing, described casing inner bottom part is provided with electric heating pipe, described electric heating pipe is connected with the controller being arranged on described box top, and described controller is also connected with the described electric up-down bar being arranged on described box top, thermoswitch and timing register.
Further, described heat-conducting medium is any one in water or oil.
Advantage of the present utility model: 1, accurate controlled enzymatic hydrolysis system temperature, ensures that enzyme digestion reaction carries out at a suitable temperature, it is to increase the reaction efficiency of enzyme; 2, electric up-down bar coordinates action with timing register by controller, at the end of enzyme digestion reaction, is risen by all placement cups in time, avoids cytoactive to reduce because enzymolysis time crosses long; 3, reduce the artificial degree of participation in enzymolysis process, hand labor intensity can be reduced on the one hand, it is to increase working efficiency, avoid manual operation to be taken out of by enzymatic hydrolysis system reagent on the other hand and place the outer test for contamination environment of cup.
Accompanying drawing illustrates:
Fig. 1 is the utility model one-piece construction schematic diagram.
Fig. 2 is side-view of the present utility model.
Fig. 3 is the structural representation placing cup.
Casing 1, groove 2, places cup 3, filter membrane 4, electric up-down bar 5, closure frame 6, envelope lid 7, heat-conducting medium 8, electric heating pipe 9, controller 10, thermoswitch 11, timing register 12.
Embodiment:
As Figure 1-3, enzymolysis isolated cell device, it comprises the casing 1 of hollow, casing 1 top is provided with some to the groove 2 of casing 1 inner recess, it is inserted with one in each groove 2 and places cup 3, place and be provided with filter membrane 4 bottom cup 3, place cup 3 top and be provided with screw thread; Casing 1 end face is arranged with between one pair of electric up-down bar 5, two electric up-down bar 5 and it is provided with closure frame 6, the corresponding envelope lid 7 placed cup 3 and be fixed with and place cup 3 spiral shell and connect on closure frame 6; Being filled with heat-conducting medium 8 in casing 1, heat-conducting medium 8 is oil; Casing 1 inner bottom part is provided with electric heating pipe 9, and electric heating pipe 9 is connected with the controller 10 being arranged on casing 1 end face, and controller 10 is also connected with the electric up-down bar 5 being arranged on casing 1 end face, thermoswitch 11 and timing register 12.
Using method:
First, thermoswitch 11 regulates the Heating temperature of electric heating pipe 9 by controller 10, and makes the temperature around each groove 2 of casing 1 rise to the temperature needed for enzyme digestion reaction by heat-conducting medium 8; Required various enzymolysis reagent used is added in groove 2, embryonic tissue is put into and places cup 3, placement cup 3 is screwed onto on the envelope lid 7 on closure frame 6 and opens timing register 12, controller 10 controls electric up-down bar 5 and falls, making placement cup 3 immerse in enzymatic hydrolysis system, embryonic tissue carries out enzymolysis when constant temperature in enzymatic hydrolysis system; At the end of timing register 12 timing, controller 10 controls electric up-down bar 5 and rises, placing cup 3 is moved out of outside groove 2, place the various enzymolysis reagent in cup 3 by placing filter membrane 4 filtering bottom cup 3, the individual cells being separated into by embryonic tissue is stayed and is placed in cup 3, placement cup 3 is taken off from envelope lid 7, individual cells is carried out follow-up test from placing to take out cup 3.
In the process of whole embryonic tissue enzymolysis separation, thermoswitch 11 regulates the Heating temperature of electric heating pipe 9 by controller 10, makes the temperature of enzymatic hydrolysis system precisely controlled, ensures that enzyme digestion reaction carries out at a suitable temperature, it is to increase the reaction efficiency of enzyme; Timing register 12 controls the lifting of electric up-down bar 5 by controller 10, and the process of enzymolysis isolated cell can be terminated in time, avoids cytoactive to reduce because enzymolysis time crosses long; Enzyme digestion reaction terminate after by place the disposable taking-up individual cells of cup 3, it is to increase cell picks up efficiency; Meanwhile, the present embodiment can reduce the artificial degree of participation in enzymolysis process, can reduce hand labor intensity on the one hand, it is to increase working efficiency, avoids manual operation to be taken out of by enzymatic hydrolysis system reagent on the other hand and places the outer test for contamination environment of cup 3.
The foregoing is only better embodiment of the present utility model; not in order to limit the utility model; all within spirit of the present utility model and principle, any amendment of doing, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (2)
1. automatic enzymolysis isolated cell device, it is characterised in that, it comprises the casing of hollow, described casing top is provided with some the grooves caved in described box house, being inserted with one in groove described in each and place cup, be provided with filter membrane bottom described placement cup, described placement cup top is provided with screw thread; Described box top is arranged with one pair of electric up-down bar, described in two, between electric up-down bar, is provided with closure frame, the envelope lid that on described closure frame, corresponding described placement cup is fixed with described placement cup spiral shell connects; It is filled with heat-conducting medium in described casing, described casing inner bottom part is provided with electric heating pipe, described electric heating pipe is connected with the controller being arranged on described box top, and described controller is also connected with the described electric up-down bar being arranged on described box top, thermoswitch and timing register.
2. automatic enzymolysis isolated cell device according to claim 1, it is characterised in that, described heat-conducting medium is any one in water or oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521026285.2U CN205275570U (en) | 2015-12-12 | 2015-12-12 | Automatic enzymolysis isolated cell device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521026285.2U CN205275570U (en) | 2015-12-12 | 2015-12-12 | Automatic enzymolysis isolated cell device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205275570U true CN205275570U (en) | 2016-06-01 |
Family
ID=56060163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521026285.2U Active CN205275570U (en) | 2015-12-12 | 2015-12-12 | Automatic enzymolysis isolated cell device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205275570U (en) |
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2015
- 2015-12-12 CN CN201521026285.2U patent/CN205275570U/en active Active
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |