CN205175759U - Detection device and detect chip thereof - Google Patents
Detection device and detect chip thereof Download PDFInfo
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- CN205175759U CN205175759U CN201520745936.7U CN201520745936U CN205175759U CN 205175759 U CN205175759 U CN 205175759U CN 201520745936 U CN201520745936 U CN 201520745936U CN 205175759 U CN205175759 U CN 205175759U
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- whole blood
- separation
- blood portion
- detection chip
- lipid
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Abstract
The utility model discloses a detect the chip, including the whole blood separation portion that is used for holding the whole blood sample, whole blood separation portion is by the bottom to the axis of port for the line of bending, the port terminal surface of whole blood separation portion with the axis slope of whole blood separation portion sets up. Above -mentioned device when using, with in the whole blood sample leading -in whole blood separation portion, the effect of the centrifugal force through different angles, make the lipid centrifugal in -process and the separation of blood sample and spill over with whole blood separation portion for only leave plasma and blood cell in the whole blood separation portion, ensured do not contain the lipid in remaining blood, thereby ensured the accuracy to the blood testing. The utility model also provides an including the above -mentioned detection device who detects the chip, this detection device detects the chip through the aforesaid to carry out the centrifugation to the lipid of whole blood sample median and gets rid of, has ensured the accuracy of blood testing.
Description
Technical field
The utility model relates to technical field of medical instruments, particularly a kind of detection chip.In addition, the utility model also relates to a kind of pick-up unit comprising above-mentioned detection chip.
Background technology
In modern medicine study, measuring method has a very important role in clinical biochemical detects.
Measuring method can be divided three classes according to optical principle: absorption spectroanalysis method, emission spectrometry method and scattering spectrum analytic approach.
The blood of hyperlipidemia or hyperlipemia patient with sympotoms is by after centrifugal, and a large amount of insoluble lipids has been stayed in blood plasma.This lipid be suspended can hinder the carrying out of detection, causes testing result inaccurate.Such as, in absorption spectrum Epidemiological Analysis and scattering spectrum are analyzed, the turbidity of lipid can increase extra absorbance; And in emission spectrometry method, lipid will absorb exciting light and utilizing emitted light.That is, this hyperlipoidemia can affect the detection detecting spectroscopy.Therefore, detect in the blood being mixed with lipid, will be difficult to obtain accurate testing result.
In order to solve the interference of piarhemia, conventional method is reagent blank calibration method.The method can eliminate the interference of piarhemia to a certain extent, but add the step of test in this process, increase sample use amount, and cause operating process more loaded down with trivial details, and at POCT (PointofCareTesting, instant detection) in process, be then difficult to realize above-mentioned testing procedure.In addition, also have a kind of mode to be add surfactant lipid to good solubilization in detection reagent, make Lipid dissolution in blood plasma in blood plasma.But the surfactant that the method is added can increase the risk of haemolysis, and adverse influence is produced to the enzymatic activity that some detects in reagent, bring uncertainty, affect testing result, the impact that aforesaid way all can not well avoid lipid to bring.
Therefore, the impact how avoiding lipid to bring to the detection of blood sample is the current technical issues that need to address of those skilled in the art.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of detection chip, and this detection chip is by removing the lipid in blood by centrifugal process.
Another object of the present utility model is to provide a kind of pick-up unit comprising above-mentioned detection chip, and the Detection results of this pick-up unit is good, avoids the error that lipid brings.
For achieving the above object, the utility model provides following technical scheme:
A kind of detection chip, comprises the separation of whole blood portion for holding whole blood sample, and described separation of whole blood portion is bending line by bottom to the axis of port, and the port end face in described separation of whole blood portion and the described axis in described separation of whole blood portion are obliquely installed.
Preferably, described separation of whole blood portion comprises at least two separating layers on the direction perpendicular to described axis, and the port in described separation of whole blood portion is provided with the intercommunicating pore being communicated with described separating layer.
Preferably, described separation of whole blood portion comprises two described separating layers altogether.
Preferably, a sidewall in described separation of whole blood portion is provided with the depressed part caved in inside described separation of whole blood portion.
Preferably, the longitudinal section of described depressed part is trapezoidal, and the upper end of described depressed part flushes with described port.
Preferably, the bending angle scope in described separation of whole blood portion is 120 to 160 degree.
A kind of pick-up unit, comprise centrifugal device and detection chip, described detection chip is the detection chip as described in above-mentioned any one.
Preferably, described pick-up unit comprises sample introduction part and reagent storage portion.
Whole blood sample in use, first, imports in separation of whole blood portion by centrifugal action by the detection chip that the utility model provides, by importings as far as possible many for blood wherein.Then, by the effect of the centrifugal force of different angles, change the actual volume in separation of whole blood portion, the lipid making density less is separated with blood sample and overflows and separation of whole blood portion in centrifugal process, make only to be left blood plasma and haemocyte in separation of whole blood portion, extracting blood plasma by identical way again, leaving the haemocyte for detecting.Ensure that not containing lipid in remaining blood, thus ensured the accuracy to blood testing.Actual volume in the separation of whole blood portion that centrifugal process is formed constantly reduces, thus ensure that the overflow of lipid.
The utility model additionally provides a kind of pick-up unit comprising above-mentioned detection chip, and this pick-up unit carries out centrifugal segregation by above-mentioned detection chip to the lipid in whole blood sample, has ensured the accuracy of blood testing.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only embodiment of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to the accompanying drawing provided.
Fig. 1 provide by the utility model separation of whole blood portion first time in specific embodiment one centrifugal after longitudinal sectional view;
Fig. 2 provide by the utility model separation of whole blood portion second time in specific embodiment one centrifugal after longitudinal sectional view;
Fig. 3 provide by the utility model separation of whole blood portion first time in specific embodiment two centrifugal after longitudinal sectional view;
Fig. 4 provides by the utility model the cut-open view of separating layer in specific embodiment two;
Fig. 5 provide by the utility model separation of whole blood portion first time in specific embodiment three centrifugal after longitudinal sectional view;
Fig. 6 provide by the utility model separation of whole blood portion second time in specific embodiment three centrifugal after longitudinal sectional view.
In Fig. 1 to Fig. 6,1 is separation of whole blood portion, and 2 is intercommunicating pore, and 3 is depressed part, and 4 is haemocyte, and 5 is blood plasma, and 6 is lipid.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
Core of the present utility model is to provide a kind of detection chip, and this detection chip is by removing the lipid in blood by centrifugal process.Another core of the present utility model is to provide a kind of pick-up unit comprising above-mentioned detection chip, and the Detection results of this pick-up unit is good, avoids the error that lipid brings.
Please refer to Fig. 1 to Fig. 6, Fig. 1 provide by the utility model first time in specific embodiment one centrifugal after longitudinal sectional view; Fig. 2 provide by the utility model second time in specific embodiment one centrifugal after longitudinal sectional view; Fig. 3 provide by the utility model first time in specific embodiment two centrifugal after longitudinal sectional view; Fig. 4 provides by the utility model the cut-open view of separating layer in specific embodiment two; Fig. 5 provide by the utility model first time in specific embodiment three centrifugal after longitudinal sectional view; Fig. 6 provide by the utility model second time in specific embodiment three centrifugal after longitudinal sectional view.
Detection chip provided by the utility model is mainly used on blood test device, bears the work being separated blood and testing.
Above-mentioned detection chip mainly comprises separation of whole blood portion 1, and the Main Function in separation of whole blood portion 1 holds blood sample to be separated.Separation of whole blood portion 1 is for having the kit of one fixed width, and separation of whole blood portion 1 is generally flat structure, can be the kit of rectangle or other shapes, and except one end has port, other positions are enclosed construction.As the separation of whole blood portion 1 of the container of placement blood sample, its bottom has certain distance between port, and along the main body in separation of whole blood portion 1, be bending line by bottom to the axis of port, and the port end face in separation of whole blood portion 1 and above-mentioned axis are for being obliquely installed, namely end face and the relation of axis be not vertical neither be parallel, but there is the position relationship of certain slanted angle.
Separation of whole blood portion 1 comprises a variety of situation particularly, please refer to Fig. 1 and Fig. 2.In an embodiment of the present utility model, the longitudinal section in separation of whole blood portion 1 is similar to the test tube cross section of a bending, separation of whole blood portion 1 is the structure extended transversely by above-mentioned longitudinal sectional structure, be different from common reagent card, port plane in the present embodiment and the central shaft in separation of whole blood portion 1 out of plumb, namely the Sidewall Height of port is not identical.
Need it is to be noted that the axis mentioned in the application is that separation of whole blood portion 1 is by the extending longitudinally axis of container bottom to port.
Whole blood sample in use, first, imports in separation of whole blood portion 1 by centrifugal action by the detection chip provided in the present embodiment, by importings as far as possible many for blood wherein, can ensure the smooth derivation of centrifugal process lipid.
Then, first centrifugal force is applied to separation of whole blood portion 1, the direction of the first centrifugal force can for pointing to the direction on the right side in Fig. 1, first time, centrifugal formation was later as the centrifugal effect of Fig. 1, owing to mainly comprising the principal ingredients such as haemocyte 4, blood plasma 5 and lipid 6 in whole blood sample, and the density of these compositions is not etc., centrifugal complete after will form effect according to density stratification in separation of whole blood portion 1, what be wherein positioned at separation of whole blood portion 1 bottom is haemocyte 4, the part adjacent with haemocyte 4 is blood plasma 5, is then lipid 6 in outermost part.Due to centrifugal according to above-mentioned direction, the actual capacity in separation of whole blood portion 1 can be less than the volume in separation of whole blood portion 1, so unnecessary blood is then overflowed by port, lipid 6 part can be isolated in the outermost layer in separation of whole blood portion 1 by this step.
Then, second centrifugal force is applied to separation of whole blood portion 1, the lower right in Fig. 2 can be pointed in the direction of the second centrifugal force, due to time centrifugal along this direction, the actual capacity in separation of whole blood portion 1 reduces again, so be subject to centrifugal action at outermost lipid 6, overflowed by the port in separation of whole blood portion 1, thus the blood sample obtained not containing lipid 6, wherein only containing blood plasma 5 and haemocyte 4, and haemocyte 4 is still in the position near bottom, separation of whole blood portion 1.
Finally, the conveniently detection of subsequent step, applies the 3rd centrifugal force to separation of whole blood portion 1, and the blood plasma 5 being in middle part is derived from separation of whole blood portion 1, and haemocyte 4 is still retained in separation of whole blood portion 1.
Needs should be mentioned that, centrifugal direction in above-mentioned implementation process is not unique, can know that the port that said process make use of separation of whole blood portion 1 is the feature of tilt port, in the process passing through different directions centrifugal action, lipid 6 part near port is overflowed by port, so three centrifugal directions can have multiple choices, the actual volume in the separation of whole blood portion 1 that three centrifugal processes are formed constantly reduces, thus ensure that the overflow of lipid 6.Above-mentioned centrifugal process can be realized by hydro-extractor or rotation detection portion.
Above-described embodiment provide the separation of whole blood portion 1 in detection chip to be the container of a bending, this container is bending line by bottom to the axis of port, and the port in this separation of whole blood portion 1 is for being obliquely installed, ensure that under the centrifugal action of different directions, the capacity in separation of whole blood portion 1 changes, lipid 6 is mainly separated by said process from whole blood, thus achieve the separation of different piece in blood sample, obtain the haemocyte 4 not containing lipid 6, thus ensure that the accurate of testing result.
Optionally, the bending angle scope in separation of whole blood portion 1 is 120 to 160 degree.The container being set to separation of whole blood portion 1 to have certain bending angle is in order in centrifugal process, and bending structure can avoid the spilling of internal blood, so neither can be too straight in angle, can not bending larger.
For overflow direction and the receive mode of lipid in above-described embodiment 6, in another embodiment provided by the utility model, on the basis of said structure, separation of whole blood portion 1 is being comprised at least two separating layers perpendicular on the direction of axis, and the port in separation of whole blood portion 1 is provided with the intercommunicating pore 2 being communicated with separating layer.Above-mentioned at least two separating layers should be understood to comprise two separating layers, three multiple situations such as separating layer and multiple separating layers.
Particularly, please refer to Fig. 3 and Fig. 4, Fig. 3 and Fig. 4 be respectively the utility model to provide in specific embodiment two first time centrifugal after longitudinal sectional view and the structural representation of separating layer.
Separation of whole blood portion 1 has two separating layers on the direction vertical with axis, and Fig. 4 at the middle and upper levels runner is the principal space for placing blood sample, and lower floor's runner is for depositing the lipid 6 and blood plasma 5 that overflow.Be communicated with by intercommunicating pore 2 between two runners, intercommunicating pore 2 is arranged on the port in separation of whole blood portion 1.
In the present embodiment, separation of whole blood portion 1 is divided into two-layer in a thickness direction, when being in the blood sample in the runner of upper strata and being separated, lipid 6 can be overflowed by intercommunicating pore 2, simultaneously due to the effect of centrifugal force, entered in lower floor's runner by intercommunicating pore 2, and flow into bottom it.In said process, ensure that the lipid 6 of overflow place or the preservation of blood plasma 5 and placement.
Need, it is to be noted that the number of separating layer can be adjusted to three layers or more according to centrifugal requirement in separation of whole blood portion 1, to be not limited to scheme two-layer in above-described embodiment.
In an embodiment of the present utility model, a sidewall in separation of whole blood portion 1 is provided with the depressed part 3 of depression inside separation of whole blood portion 1, meanwhile, the port in separation of whole blood portion 1 and axis keep being obliquely installed.During use, first, separation of whole blood portion 1 is set to depressed part 3 substantially towards upper angle, and the port in separation of whole blood portion 1 upward, as shown in Figure 5, apply the first downward centrifugal force, under the effect of the first centrifugal force, whole blood is divided into haemocyte 4, blood plasma 5, lipid 6, formed as the blood layering in Fig. 5, and unnecessary blood is overflowed by port; Then, as shown in Figure 6, the centrifugal force to the lower left in Fig. 6 is applied, i.e. direction at the bottom of bottle.Second time is centrifugal makes the actual volume in separation of whole blood portion 1 be less than first time centrifugal actual volume, thus originally in outermost lipid 6 part because second time centrifugal process is separated.Finally, by mode same as the previously described embodiments, blood plasma 5 is being separated.
Can know, due to the above-mentioned depressed part 3 caved inward, causing axis is also bending line, and the separation of whole blood portion 1 so this with depressed part 3 is a kind of alternatives of above-described embodiment.That is, separation of whole blood portion 1 also not necessarily is bending container, and can be the container that sidewall has bending structure.
Needs should be mentioned that, in each centrifugation step above-mentioned, first centrifugal force all can be selected perpendicular to separation of whole blood portion 1 port direction, and the second centrifugal force can select the direction being parallel to port sidewall, by the change in twice centrifugal force direction, affect the inner actual volume change in separation of whole blood portion 1, outermost lipid is departed from.
In a specific embodiment provided by the utility model, the longitudinal section of depressed part 3 is trapezoidal, and the upper end of depressed part 3 is positioned at port, i.e. situation as shown in Figure 5 and Figure 6.
Except the detection chip that above-described embodiment provides, the utility model also provides a kind of pick-up unit comprising the open detection chip of above-described embodiment, and this pick-up unit mainly comprises centrifugal device and detection chip.The Detection results of this pick-up unit is good, avoids the impact that lipid 6 brings to testing process.
Particularly, pick-up unit also comprises sample introduction part and reagent storage portion.Certainly, also need to comprise for carrying out extracting in pick-up unit, the functional part of Detection and measure etc. and the structure of other each several parts please refer to prior art, repeat no more herein.
In this instructions, each embodiment adopts the mode of going forward one by one to describe, and what each embodiment stressed is the difference with other embodiments, between each embodiment identical similar portion mutually see.
Above a kind of pick-up unit provided by the utility model and detection chip thereof are described in detail.Apply specific case herein to set forth principle of the present utility model and embodiment, the explanation of above embodiment just understands method of the present utility model and core concept thereof for helping.Should be understood that; for those skilled in the art; under the prerequisite not departing from the utility model principle, can also carry out some improvement and modification to the utility model, these improve and modify and also fall in the protection domain of the utility model claim.
Claims (8)
1. a detection chip, comprise the separation of whole blood portion (1) for holding whole blood sample, it is characterized in that, described separation of whole blood portion (1) is bending line by bottom to the axis of port, and the port end face of described separation of whole blood portion (1) and the described axis in described separation of whole blood portion (1) are obliquely installed.
2. detection chip according to claim 1, it is characterized in that, described separation of whole blood portion (1) comprises at least two separating layers on the direction perpendicular to described axis, and the port of described separation of whole blood portion (1) is provided with the intercommunicating pore (2) being communicated with described separating layer.
3. detection chip according to claim 2, is characterized in that, described separation of whole blood portion (1) comprises two described separating layers altogether.
4. the detection chip according to claims 1 to 3 any one, is characterized in that, a sidewall of described separation of whole blood portion (1) is provided with the depressed part (3) to described separation of whole blood portion (1) inner side depression.
5. detection chip according to claim 4, is characterized in that, the longitudinal section of described depressed part (3) is trapezoidal, and the upper end of described depressed part (3) flushes with described port.
6. detection chip according to claim 5, is characterized in that, the bending angle scope of described separation of whole blood portion (1) is 120 to 160 degree.
7. a pick-up unit, comprises centrifugal device and detection chip, it is characterized in that, described detection chip is the detection chip as described in any one of claim 1 to 6.
8. pick-up unit according to claim 7, is characterized in that, described pick-up unit comprises sample introduction part and reagent storage portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520745936.7U CN205175759U (en) | 2015-09-24 | 2015-09-24 | Detection device and detect chip thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520745936.7U CN205175759U (en) | 2015-09-24 | 2015-09-24 | Detection device and detect chip thereof |
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| Publication Number | Publication Date |
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| CN205175759U true CN205175759U (en) | 2016-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520745936.7U Expired - Fee Related CN205175759U (en) | 2015-09-24 | 2015-09-24 | Detection device and detect chip thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018184382A1 (en) * | 2017-04-06 | 2018-10-11 | 美康生物科技股份有限公司 | Microfluidic chip for separating and detecting whole blood sample and detection method thereof |
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- 2015-09-24 CN CN201520745936.7U patent/CN205175759U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018184382A1 (en) * | 2017-04-06 | 2018-10-11 | 美康生物科技股份有限公司 | Microfluidic chip for separating and detecting whole blood sample and detection method thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20200924 |