CN205103164U - Electrochemiluminescence immunoassay system and flow -through cell subassembly thereof - Google Patents
Electrochemiluminescence immunoassay system and flow -through cell subassembly thereof Download PDFInfo
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- CN205103164U CN205103164U CN201520849326.1U CN201520849326U CN205103164U CN 205103164 U CN205103164 U CN 205103164U CN 201520849326 U CN201520849326 U CN 201520849326U CN 205103164 U CN205103164 U CN 205103164U
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Abstract
The utility model discloses an electrochemiluminescence reaction takes place for electrochemiluminescence immunoassay system and flow -through cell subassembly thereof, the liquid that awaits measuring in the flow -through cell subassembly, the flow -through cell subassembly includes working electrode, to electrode and reference electrode, working electrode with set up from top to bottom the electrode, the liquid flow path has between two electrodes. When electrochemical reaction takes place when, reactant evenly distributed in working electrode, during the test, the controlled reaction thing of being convenient for, when rinsing this flow -through cell, the liquid flow path is unblocked for the cleaning performance is better, and can avoid wasing totally and electrode that cause is ageing, is favorable to the flow -through cell subassembly to use repeatedly, and improves measuring result's accuracy greatly. Reference electrode is equipped with porous structure with the face of being connected of liquid flow path intercommunication, and this porous structure makes reference electrode have good electric conductivity, under the prerequisite of guaranteeing the reference electrode function, avoids reference electrode premature aging, improves the durability of flow -through cell subassembly.
Description
Technical field
The utility model relates to technical field of medical instruments, particularly a kind of Electrogenerated chemiluminescent immunoassay system and flow cell assembly thereof.
Background technology
After ECL (Electro-Chemiluminescence, electrochemiluminescence) refers to and apply certain voltage on electrode, some the component generation chemical reaction between the reaction product of electrode surface or in the reaction product of electrode surface and system.Course of reaction is divided into electrochemical reaction process, chemiluminescence and cyclic process three phases.
Electrochemiluminescence immunoassay technology is the product that electrochemiluminescence (ECL) and immunoassays combine.The principle of luminosity of its label is different from general chemiluminescence (CL), is a kind of specific chemical luminescence-producing reaction caused by galvanochemistry at electrode surface, in fact includes galvanochemistry and chemiluminescence two processes.The difference of ECL and CL is, ECL is that electricity starts luminescence-producing reaction, and CL is by the luminescence-producing reaction of compound both-initiated strategy.
ECL not only can be applied to all immunoassays, but also can be used for DNA/RNA probe in detecting, be that continue radio-immunity (RIA), enzyme immunity (EIA), fluorescence immunoassay (FIA), chemiluminescence immunoassay (CLIA) measures later label immunoassay technology of new generation.ECL technology can be applicable to the principle of panimmunity reaction, and its distinctive advantage can rely on its platform development to go out a large amount of detection reagent, comprises over one hundred items such as hormone, first merit, tumor markers, Applications of Cardiac Markers, anaemia, infectious disease.
At present, the carrier of conventional Electrochemial luminescence detecting instrument generation electrochemiluminescence reaction mainly comprises following three types, i.e. the reaction cup of reaction tank, printed electrode and flow cell.
First, in the Electrochemial luminescence detecting instrument that electrochemical reaction is carried out in reaction tank, reaction tank is easy cleaning Reusability not, and in this detector, reaction electrode, column structure is mostly to electrode and contrast electrode, comparatively be applicable to a small amount of laboratory study detected, be not suitable for large-scale clinical detection.
Secondly, for the Electrochemial luminescence detecting instrument three electrodes are printed in reaction cup, there is electrochemiluminescence reaction in three electrodes, this reaction cup can only use once in reaction cup, because electrode material is worth higher, and single use high cost.In addition, need to soak in a liquid before contrast electrode uses and when using, the reference effect that competence exertion is best, the reaction cup of printed electrode makes contrast electrode expose to the open air at dry environment, easily oxidation and aging.
Finally, that commonly uses at present is provided with in the Electrochemial luminescence detecting instrument of flow cell, and the working electrode of flow cell is arranged at the central authorities of electrode disk, electrode retaining collar is around in the periphery of working electrode, and two electrodes are arranged at same level.When the flow cell of this structure cleans, cleaning liquid need working electrode and in the gap between electrode around, cleaning process is difficult to carry out and cleaning performance is poor, thus makes two electrodes easily aging, affects the accuracy of measurement result and reduces serviceable life of flow cell.
In view of the defect that above-mentioned flow cell exists, urgently provide a kind of flow cell can Reusability, electrode not easily aging, measurement result flow cell more accurately.
Utility model content
For solving the problems of the technologies described above, first object of the present utility model is for providing a kind of flow cell assembly of Electrogenerated chemiluminescent immunoassay system, in this flow cell assembly, to electrode and working electrode setting up and down, conveniently clean this flow cell assembly and clean without dead angle, avoid not washing clean clearly and the ageing of electrode caused, thus greatly improve the accuracy of measurement result.
Second object of the present utility model is for providing a kind of Electrogenerated chemiluminescent immunoassay system comprising this flow cell assembly.
For realizing the first object of the present utility model, the utility model provides a kind of flow cell assembly of Electrogenerated chemiluminescent immunoassay system, electrochemiluminescence reaction is there is in testing liquid in described flow cell assembly, described flow cell assembly comprises working electrode and to electrode, described working electrode and described setting up and down to electrode.
In the utility model, working electrode is with setting up and down to electrode, when cleaning this flow cell assembly, cleaning liquid do not need between electrodes around, conveniently clean and clean without dead angle, make cleaning performance better, avoid electrode clean clean and the ageing of electrode that causes, thus greatly improve the accuracy of measurement result.
Alternatively, describedly electrode is comprised to two needle electrodes be located at above described working electrode, described working electrode is pellet electrode.
Alternatively, described electrode and described working electrode to be located in flow cell, and described flow cell has the liquid flow path for liquid communication;
Also comprise the contrast electrode being located at described flow cell side, described working electrode, described liquid flow path between electrode and described contrast electrode to be connected, described contrast electrode comprises the 3rd active section be connected with liquid flow path, and its joint face be communicated with liquid flow path is provided with porous structure.
Alternatively, described flow cell is formed by the upper plate cooperatively interacted and lower plate, and described upper plate is made up of transparent material, is describedly fixed on described upper plate to electrode, and described working electrode is fixed on described lower plate.
Alternatively, sealing gasket is provided with between described upper plate and described lower plate, and it offers the first through hole, described first through hole and described lower plate and described upper plate forming reactions chamber, the described lower plate being positioned at described reaction chamber offers inlet and liquid outlet, and electrochemiluminescence reaction occurs testing liquid in described reaction chamber.
Alternatively, described upper plate, described sealing gasket and described lower plate all offer the threaded hole be interconnected.
Alternatively, describedly comprise the first active section to electrode, described working electrode comprises the second active section, described first active section and described second active section forming reactions loop.
Alternatively, described first active section at least one end bends along the direction towards described upper plate, and form the first hook, described upper plate offers the first pocket for the punch head linked up with described first and coordinate, and described first hook is connected in described first pocket for the punch head;
Described second active section at least one end bends along the direction towards described lower plate, and form the second hook, described lower plate is provided with the second through hole linked up with described second and coordinate, and described second hook is connected in described second through hole.
In order to realize the second object of the present utility model, the utility model provides a kind of Electrogenerated chemiluminescent immunoassay system, comprise flow cell assembly and the detection components for detecting described flow cell assembly, also comprise the Control Component for controlling described flow cell component operation and the fixed head for fixing described flow cell assembly and described Control Component, described flow cell assembly is above-described flow cell assembly.
Alternatively, described Control Component comprises pivoted arm and stepper motor, and one end of described pivoted arm is articulated with described stepper motor, and rotate to control described pivoted arm, the other end of described pivoted arm connects magnet, is adsorbed in described working electrode with the magnetic bead controlled in testing liquid.
Accompanying drawing explanation
Fig. 1 is the structural representation of flow cell assembly;
Fig. 2 is the front elevation of Fig. 1;
Fig. 3 is the upward view of Fig. 1;
Fig. 4 is the structural representation removing upper plate in Fig. 1;
Fig. 5 is the cut-open view of contrast electrode in Fig. 1;
Fig. 6 is that the A-A of Fig. 2 is to cut-open view;
Fig. 7 is that the B-B of Fig. 2 is to cut-open view;
Fig. 8 provides by the utility model the structural representation of Electrogenerated chemiluminescent immunoassay system;
Fig. 9 is the front elevation of Fig. 8.
In Fig. 1-9:
1 flow cell assembly, 11 pairs of electrodes, 111 first active sections, 112 first hooks;
12 working electrodes, 121 second hooks, 122 second active sections;
13 contrast electrodes, 131 contrast electrode shells, 132 the 3rd active sections;
14 upper plates, 15 lower plates, 151 second through holes;
16 sealing gaskets, 161 first through holes, 162 arcuate structures, 17 grooves;
2 Control Components, 21 pivoted arms, 22 stepper motors, 23 magnet;
3 detection components, 4 fixed heads.
Embodiment
In order to make those skilled in the art understand the technical solution of the utility model better, below in conjunction with the drawings and specific embodiments, the utility model is described in further detail.
Please refer to accompanying drawing 1-6, wherein, Fig. 1 is the structural representation of flow cell assembly; Fig. 2 is the front elevation of Fig. 1; Fig. 3 is the upward view of Fig. 1; Fig. 4 is the structural representation removing upper plate in Fig. 1; Fig. 5 is that the A-A of Fig. 2 is to cut-open view; Fig. 6 is that the B-B of Fig. 2 is to cut-open view.
In a kind of specific embodiment, the utility model provides a kind of flow cell assembly 1 of Electrogenerated chemiluminescent immunoassay system, electrochemiluminescence reaction is there is in the testing liquid of magnetic bead coated antibody-antigen-luminous agent in this flow cell assembly 1, flow cell assembly 1 comprises working electrode 12 and to electrode 11, and this working electrode 12 is with setting up and down to electrode 11.In addition, this flow cell assembly 1 also comprises contrast electrode 13, jointly forms three-electrode system with working electrode 12 with to electrode 11, and working electrode 12 with to electrode 11 forming reactions loop, working electrode 12 and contrast electrode 13 form measure loop.
In the present embodiment, working electrode 12 is with setting up and down to electrode 11, when cleaning this flow cell, cleaning liquid do not need between electrodes around, convenient cleaning and cleaning, without dead angle, make cleaning performance better, avoid electrode clean totally and cause aging, improve the serviceable life of flow cell assembly, and greatly improve the accuracy of measurement result.Meanwhile, working electrode 12 setting up and down and make each arrangement of parts of flow cell assembly 1 compact to electrode 11, reduces floor area, reduces production cost.
Particularly, as shown in Figure 4, electrode 11 is comprised to two needle electrodes be located at above working electrode 12, working electrode 12 is rectangular patch electrode.During work, two needle electrodes apply voltage simultaneously, the negative electrode of common formation electrochemiluminescence reaction, and form response circuit with working electrode 12, working electrode 12 is powered on smooth logical, ensure that electrochemiluminescence reaction occurs and circulates on working electrode 12, can not the reaction on working electrode 12 be impacted meanwhile.
In the present embodiment, working electrode 12 is ganoid rectangular patch electrode, when the testing liquid of magnetic bead coated antibody-antigen-luminous agent passes through, more testing liquid can be adhered in working electrode 12 surface, and testing liquid is evenly distributed, when there is electrochemiluminescence reaction, improve the efficiency of electrochemiluminescence reaction, when having reacted rear test, equally distributed determinand is convenient to control, and improves the accuracy of testing result.
Simultaneously, be needle electrode to electrode 11, photomultiplier is connected above it, by photomultiplier measurement working electrode 12 reacting the photon of generation, therefore, can avoid during to electrode 11 in needle-like stopping electrode 11 and light path between working electrode 12 and photomultiplier ensure the accuracy of testing result.In addition, in order to ensure, to electrode 11, there is enough large area, make to comprise two coefficient needle electrodes to electrode 11.
In addition, be located in flow cell to electrode 11 and working electrode 12, this flow cell has the liquid flow path for liquid communication, contrast electrode 13 is located at the side of flow cell, and electrode 11, liquid flow path between working electrode 12 with contrast electrode 13 three are connected, this contrast electrode 13 comprises the 3rd active section 132 be connected with liquid flow path, and its joint face be communicated with liquid flow path is provided with porous structure, under the prerequisite ensureing signal communication, the liquid corrosion contrast electrode 13 of the soda acid composition in fluid path can be prevented.
3rd active section 132 is placed in contrast electrode shell 131, in addition, the interior also conducting solution such as accommodating potassium chloride or sodium chloride of this contrast electrode shell 131, the 3rd active section 132 caused to prevent Long-Time Service is oxidized, 3rd active section 132 need be soaked in conducting solution for a long time, therefore, during in order to ensure that in contrast electrode shell 131, conducting solution is less, 3rd active section 132 still can be soaked in wherein, 3rd active section 132 is set to spiral fashion (as shown in Figure 5), or cross section is waveform, the electrode of other shape such as triangle, thus effectively prevent the 3rd active section 132 to be oxidized, make control comparisons value stabilization in testing process reliable, the accuracy of further raising testing result, improve the serviceable life of flow cell assembly simultaneously.
Certainly, working electrode 12, the structure of electrode 11 and contrast electrode 13 is not limited in this, the xsect of working electrode 12 also can be other random geometry such as circle, polygon, other random geometry such as sheet, spiral fashion be can be to electrode 11,3rd active section 132 of contrast electrode 13 also can be the conventional needle electrode in this area, but, based on improving electrochemiluminescence reaction efficiency, do not affect signals collecting and prevent the object of anodizing, working electrode 12, structure electrode 11 and contrast electrode 13 be preferably in the present embodiment.
In addition, working electrode 12, can be made up of various materials such as gold, platinum, graphite, silver, silver chlorides electrode 11 and contrast electrode 13, because platinum ductility is better, various shape can be processed as, therefore, in order to improve the dirigibility of each electrode structure, each electrode in the present embodiment preferably adopts platinum electrode.The porous structure of the joint face that the 3rd active section 132 is communicated with liquid flow path can be the conventional porous structures such as porous ceramics.
Further, as shown in figures 1 to 6, this flow cell assembly 1 also comprises the upper plate 14 and lower plate 15 that cooperatively interact, and this upper plate 14 and lower plate 15 form flow cell.Wherein, upper plate 14 is made up of transparent optical glass, and electrochemiluminescence reacts this upper plate 14 of light transmission sent.Meanwhile, be fixed on upper plate 14 to electrode 11, working electrode 12 is fixed on lower plate 15, and working electrode 12 and have vertical gap between electrode 11, and this vertical gap forms working electrode 12 and to the liquid flow path between electrode 11.
As shown in Figure 4, this flow cell assembly 1 comprises the sealing gasket 16 being located at working electrode 12 surface further, and it offers the first through hole 161, this first through hole 161 and lower plate 15 and upper plate 14 forming reactions chamber, and sealing gasket 16 seals this reaction chamber, prevents leak of liquid; Meanwhile, the first through hole 161 is the little fusiformis broad in the middle in sectional area two, and two of this fusiformis first through hole 161 is respectively equipped with inlet and liquid outlet, and testing liquid and cleaning liquid flow in this fusiformis reaction chamber.
In the present embodiment, fusiformis reaction chamber volume is less, when testing liquid and flushing liquid flow in this reaction chamber, fluid near linear flows, and does not need convolution, therefore, during reaction, testing liquid can fully contact with to electrode 11 with working electrode 12, is conducive to reaction and carries out smoothly.The more important thing is, when cleaning electrode, after cleaning liquid enters from inlet, flow out smoothly from liquid outlet, cleaning liquid is near linear flowing in reaction chamber, and cleaning, without dead angle, effectively can prevent ageing of electrode.
Be appreciated that, first through hole 161 of sealing gasket 16 is not limited in as the little fusiformis structure broad in the middle in two, cross section, also can be along the identical rectangular configuration of liquid flow direction sectional area, but, when this first through hole 161 is fusiformis, the sectional area at inlet and liquid outlet place is less relative to other parts, thus improves the flow velocity of inlet and liquid outlet place liquid, testing liquid and flushing liquid is entered fast and flows out this reaction chamber.
Further, as shown in Figure 4, upper plate 14, sealing gasket 16 offer some threaded holes with lower plate 15, each threaded hole all coordinates with bolt, wherein, upper plate 14, sealing gasket 16 connect as one with lower plate 15 by part bolt, form flow cell assembly 1, flow cell assembly 1 is connected with the detection components 3 of the side of being located thereon by another part bolt, and this detection components 3 detects the light penetrating upper plate 14.
In addition, as shown in Figure 4, in the middle part of sealing gasket 16 be fusiformis structure, above-mentioned fusiformis first through hole 161 is offered at the middle part of this fusiformis structure, and the periphery wall of the first through hole 161 is outwardly, form some arcuate structures 162, above-mentioned each threaded hole is opened in this arcuate structure 162.In embodiment shown in Fig. 1, upper plate 14, lower plate 15 and sealing gasket 16 offer 4 threaded holes altogether, wherein, two for connecting upper plate 14, lower plate 15 and sealing gasket 16, another two for flow cell assembly 1 is connected with detection components 3.Certainly, quantity and the setting position of threaded hole are not limited in this, can arrange arbitrarily as required, be not construed as limiting herein.
In addition, the shape of sealing pad 16 is not limited in this, also can be and upper plate 14 and the suitable circle of lower plate 15, wherein, fusiformis first through hole 161 is opened in the middle part of circular configuration, some threaded holes are opened in the both sides of this first through hole 161, but the material required for sealing gasket 16 in the present embodiment is minimum, the more important thing is, the absorption of sealing gasket 16 to light can be reduced to greatest extent, thus improve the accuracy of testing result.
On the other hand, as shown in Figure 4, the first active section 111 is comprised to electrode 11, working electrode 12 comprises the second active section 122, this the first active section 111 and second active section 122 forming reactions loop, in addition, the area of the second active section 122 is less than the area of the first through hole 161.Setting like this, makes each part dimension of flow cell assembly 1 less further, is conducive to the miniaturization of equipment.
Simultaneously, first active section 111 connects the first hook 112 extended towards the direction of upper plate 14, and correspondingly, upper plate 14 is provided with links up with 112 the first pocket for the punch heads coordinated with first, first hook 112 is connected in this first pocket for the punch head, thus will be fixed on upper plate 14 to electrode 11.
Particularly, as shown in Figure 4 and Figure 5, the two ends of the first active section 111 all bend along the direction towards upper plate 14, and form two first hooks 112, upper plate 14 is provided with links up with 112 two the first pocket for the punch heads coordinated with two first; And the first hook 112 is inverted "L" shaped hook, correspondingly, the first pocket for the punch head is the inverted "L" shaped pocket for the punch head suitable with inverted "L" shaped hook.
Owing to being two needle electrodes to electrode 11, and its material is generally platinum, therefore, bendable is converted into various shape, and when it is fixed on upper plate 14, two ends are bent to inverted "L" shaped hook, make strictly to fix electrode 11 and upper plate 14, thus ensure the stability of electrochemiluminescence reaction.
Further, as shown in Figure 5, upper plate 14 offers groove 17 with the periphery of lower plate 15; Two first pocket for the punch heads extend upwardly to the upper surface of upper plate 14, and any one in two first pocket for the punch heads extends to groove 17, to make to extend out to groove 17 to one end of electrode 11, by this groove 17, electrode 11 is connected with external power source, for applying voltage to electrode 11.
In embodiment shown in Fig. 4 and Fig. 6, working electrode 12 is mutually vertical with the first through hole 161 of sealing gasket 16, and its second active section 122 is bent to form the second hook 121 towards the direction of lower plate 15, correspondingly, lower plate 15 is provided with links up with 121 the second through holes 151 coordinated with second, second hook 121 is connected in this second through hole 151, thus working electrode 12 is fixed on the upper surface of lower plate 15.In addition, be connected with power supply, for working electrode 12 applies voltage by this second through hole 151, second hook 121.
Be appreciated that the shape of the first hook 112 and the second hook 121 is not limited in this, also can be other shape that this area is conventional, as long as realize the connection to electrode 11 and upper plate 14, working electrode 12 and lower plate 15.Certainly, be also not limited in the connection of electrode 11 and upper plate 14, working electrode 12 and lower plate 15 and adopt clamping to connect, also can be other connected mode that this area is conventional, therefore, the shapes, setting position etc. of connected mode and two hooks are all not construed as limiting.
In addition, the lower plate 15 in this embodiment is made up, for the protection of each electrode of an etch-proof gram material with contrast electrode shell 131.
Please continue to refer to accompanying drawing 7 and 8, wherein, the structural representation of Fig. 7 provide to by the utility model Electrogenerated chemiluminescent immunoassay system; Fig. 8 is the front elevation of Fig. 7.
As shown in Figure 5 and Figure 6, the utility model also provides a kind of Electrogenerated chemiluminescent immunoassay system, comprise flow cell assembly 1 and the detection components 3 for detecting flow cell assembly 1, also comprise for controlling Control Component 2 that flow cell assembly 1 works and the fixed head 4 for fixing flow cell assembly 1 and Control Component 2, wherein, flow cell assembly 1 is the flow cell assembly 1 described in above any embodiment.Because above-mentioned flow cell assembly 1 has above-mentioned technique effect, the Electrogenerated chemiluminescent immunoassay system with this flow cell assembly 1 also has identical technique effect, repeats no more herein.
Wherein, as shown in FIG. 7 and 8, Control Component comprises pivoted arm 21, and one end of this pivoted arm 21 is articulated with stepper motor 22, the other end connects magnet 23, correspondingly, the bottom of flow cell assembly 1 lower plate 15 offers a groove, when stepper motor 22 control pivoted arm 21 turn to this groove time, magnet 23 enters this groove, and being positioned at below working electrode 12, the magnetic bead controlled in testing liquid is adsorbed in working electrode 12, starts electrochemiluminescence reaction.In addition, in order to ensure that abundant magnetic bead is adsorbed in working electrode 12, magnet 23 adopts permanent magnet.
Certainly, this Control Component 2 is not must control pivoted arm 21 by stepper motor 22 to realize yet, also the toggle that this area is conventional can be adopted, but, Control Component 2 in the present embodiment strictly controls the movement locus of pivoted arm 21 by stepper motor 22, thus improves the accuracy of equipment.
During the work of this electrochemical immunoanalytical test macro, first, the stepper motor 22 of Control Component 2 controls pivoted arm 21 and rotates to below working electrode 12, testing liquid is adsorbed in sheet working electrode 12 surface by the magnet 23 on pivoted arm 21, is separated by the complex of binding label ruthenium under the influence of a magnetic field with free label; Simultaneously, add containing TPA or DBAE (dibutylamino-ethanol, dibutyl amino ethanol) solution, making alive start ECL reaction, luminous substrate divalent pyridine ruthenium and TPA lose an electronics on working electrode 12 surface and are oxidized to trivalent pyridine ruthenium and kation excited state TPA
+, meanwhile, kation excited state TPA
+slough a proton and become the excited state TPA of strong reducing property, there is redox reaction in the trivalent pyridine ruthenium with strong oxidizing property and the excited state TPA with strong reducing property, trivalent pyridine ruthenium is made to be reduced to the divalent pyridine ruthenium of excited state, the divalent pyridine ruthenium of excited state decays with Fluorescence Mechanism, and release energy in the mode discharging a 620nm photon, and become the luminous substrate divalent pyridine ruthenium of ground state.
This process is carried out again and again at electrode surface, produces many photons; Finally, adopt the photomultiplier measured light intensity in detection components 3, and amplify by computer for analysis, wherein, the concentration of light intensity and pyridine ruthenium is linear, and the light intensity conversion sent on working electrode 12 according to pyridine ruthenium draws the antigen concentration in liquid to be measured.In like manner, DBAE reaction system is identical with above-described TPA reaction system principle.
Above a kind of Electrogenerated chemiluminescent immunoassay system provided by the utility model and flow cell assembly thereof are all described in detail.Apply specific case herein to set forth principle of the present utility model and embodiment, the explanation of above embodiment just understands method of the present utility model and core concept thereof for helping.Should be understood that; for those skilled in the art; under the prerequisite not departing from the utility model principle, can also carry out some improvement and modification to the utility model, these improve and modify and also fall in the protection domain of the utility model claim.
Claims (10)
1. the flow cell assembly of an Electrogenerated chemiluminescent immunoassay system, electrochemiluminescence reaction is there is in testing liquid in described flow cell assembly (1), described flow cell assembly (1) comprises working electrode (12) and to electrode (11), it is characterized in that, described working electrode (12) is with described setting up and down to electrode (11).
2. flow cell assembly according to claim 1, is characterized in that, described to electrode (11) comprise be located at described working electrode (12) top two needle electrodes, described working electrode (12) is pellet electrode.
3. flow cell assembly according to claim 1, is characterized in that, is describedly located in flow cell electrode (11) and described working electrode (12), and described flow cell has the liquid flow path for liquid communication;
Also comprise the contrast electrode (13) being located at described flow cell side, described working electrode (12), described liquid flow path between electrode (11) and described contrast electrode (13) to be connected, described contrast electrode (13) comprises the 3rd active section (132) be connected with liquid flow path, and its joint face be communicated with liquid flow path is provided with porous structure.
4. flow cell assembly according to claim 3, it is characterized in that, described flow cell is formed by the upper plate cooperatively interacted (14) and lower plate (15), described upper plate (14) is made up of transparent material, describedly be fixed on described upper plate (14) to electrode (11), described working electrode (12) is fixed on described lower plate (15).
5. flow cell assembly according to claim 4, it is characterized in that, sealing gasket (16) is provided with between described upper plate (14) and described lower plate (15), and it offers the first through hole (161), described first through hole (161) and described lower plate (15) and described upper plate (14) forming reactions chamber, the described lower plate (15) being positioned at described reaction chamber offers inlet and liquid outlet, and electrochemiluminescence reaction occurs testing liquid in described reaction chamber.
6. flow cell assembly according to claim 5, is characterized in that, described upper plate (14), described sealing gasket (16) and described lower plate (15) all offer the threaded hole be interconnected.
7. flow cell assembly according to claim 5, it is characterized in that, described first active section (111) is comprised to electrode (11), described working electrode (12) comprises the second active section (122), described first active section (111) and described second active section (122) forming reactions loop.
8. flow cell assembly according to claim 7, it is characterized in that, described first active section (111) at least one end bends along the direction towards described upper plate (14), form the first hook (112), described upper plate (14) offers the first pocket for the punch head linked up with (112) with described first and coordinate, and described first hook (112) is connected in described first pocket for the punch head;
Described second active section (122) at least one end bends along the direction towards described lower plate (15), form the second hook (121), described lower plate (15) is provided with the second through hole (151) linked up with (121) with described second and coordinate, and described second hook (121) is connected in described second through hole (151).
9. an Electrogenerated chemiluminescent immunoassay system, comprise flow cell assembly (1) and the detection components (3) for detecting described flow cell assembly (1), also comprise for controlling Control Component (2) that described flow cell assembly (1) works and the fixed head (4) for fixing described flow cell assembly (1) and described Control Component (2), it is characterized in that, the flow cell assembly (1) of described flow cell assembly (1) according to any one of claim 1-8.
10. Electrogenerated chemiluminescent immunoassay system according to claim 9, it is characterized in that, described Control Component (2) comprises pivoted arm (21) and stepper motor (22), one end of described pivoted arm (21) is articulated with described stepper motor (22), rotate to control described pivoted arm (21), the other end of described pivoted arm (21) connects magnet (23), is adsorbed in described working electrode (12) with the magnetic bead controlled in testing liquid.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105806828A (en) * | 2015-10-29 | 2016-07-27 | 北京联众泰克科技有限公司 | Electrochemical luminescence immunoassay system and circulation pool component thereof |
| CN113030070A (en) * | 2021-02-01 | 2021-06-25 | 苏州易莱生物技术有限公司 | Detection device for electrochemical luminescence detection equipment |
-
2015
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105806828A (en) * | 2015-10-29 | 2016-07-27 | 北京联众泰克科技有限公司 | Electrochemical luminescence immunoassay system and circulation pool component thereof |
| WO2017071154A1 (en) * | 2015-10-29 | 2017-05-04 | 北京联众泰克科技有限公司 | Electrochemiluminescence immunoassay system and flow-through cell component thereof |
| CN105806828B (en) * | 2015-10-29 | 2019-05-14 | 北京联众泰克科技有限公司 | A kind of Electrogenerated chemiluminescent immunoassay system and its flow cell component |
| US10976260B2 (en) | 2015-10-29 | 2021-04-13 | Beijing Unidiag Technology Inc | Electrochemiluminescence immunoassay system and flow-through cell component thereof |
| CN113030070A (en) * | 2021-02-01 | 2021-06-25 | 苏州易莱生物技术有限公司 | Detection device for electrochemical luminescence detection equipment |
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