CN205080139U - Jointly diagnose test paper subassembly - Google Patents
Jointly diagnose test paper subassembly Download PDFInfo
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- CN205080139U CN205080139U CN201520839615.3U CN201520839615U CN205080139U CN 205080139 U CN205080139 U CN 205080139U CN 201520839615 U CN201520839615 U CN 201520839615U CN 205080139 U CN205080139 U CN 205080139U
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Abstract
The utility model discloses a jointly diagnose test paper subassembly, it is including bottom plate and cutting ferrule, the top layer of bottom plate is equipped with the sample pad, combines to fill up, react the membrane and the pad that absorbs water in proper order, react epimembranal and be equipped with at least one ration area and matter accuse area in proper order, it predetermines the interval to be formed with between ration area and the matter accuse area, be equipped with the slot in the cutting ferrule, the bottom plate is inserted in the slot of locating the cutting ferrule, application of sample hole and display window have been seted up on the cutting ferrule, the application of sample hole is relative with the sample pad to be set up, by the application of sample hole adds the sample to the sample pad, display window sets up with the reaction membrane relatively, by display window presents the reaction state in ration area and matter accuse area. The utility model discloses beneficial effect such as easy to use, sensitivity is high, efficient, the accuracy is good have.
Description
Technical field
The utility model relates to biomedical testing tool, particularly relates to a kind of Combining diagnosis test paper assembly.
Background technology
In emergency treatment and Intensive Care Therapy process, assess the bacteriological infection order of severity fast and accurately significant.The introducing of C reactive protein, Procalcitonin and interleukin-6, for early infection Diagnosis and Treat reaction assessment provides effective method.Fluorescence immune chromatography is a kind of a kind of immunoassay technology be based upon on fluorescent chromatographic technology and Ag-Ab specific immune response basis, fluorescence immune chromatography test paper generally comprises to be located on base plate and the sample pad, pad, reaction film and the adsorptive pads that closely overlap successively, base plate is located in plastic clip, and plastic clip is provided with the well and display window that coordinate with sample pad and reaction film respectively.CRP is nonspecific inflammation index, is the acute stage Phasic proteins stimulating liver epithelial cell to synthesize by inflammatory lymphokine interleukin-6, il-1, tumor necrosis factor-alpha.CRP not only detects the course of disease of infectious diseases, gestational diabetes and Index for diagnosis assessment, microbiotic curative effect etc. have important potential applicability in clinical practice, is also one of most important predictor of angiocardiopathy simultaneously.In normal human serum, CRP content is atomic.General Neonatal CRP level is less than 2mg/L, is greater than this value namely relevant with the order of severity of bacteriological infection; Children and HAS CRP level are less than 10mg/L; 10-99mg/L points out focal or shallow infection, is more than or equal to 100mg/L and points out the severe infections such as septicemia or invasive infection.CRP 6-8h after infection occurs starts to raise, and 24-48h peaks, and than normal value high hundred times even thousands of times, the degree of elevation amplitude and infection is proportionate.After disease cured, its content declines rapidly, can recover normal in one week.CRP is generally as a first-selected index of discriminating bacteria or virus infections clinically, for diagnosis and the monitoring of autoimmunity and infectious diseases, and microbiotic observation of curative effect etc.Low-level CRP, also known as High-sensitivity C reactive protein.In the diagnosis of angiocardiopathy, hs-CRP is considered to the biomarker of cardiovascular inflammation pathology.Under NIP or infectious condition, it has been generally acknowledged that: be low danger during hs-CRP < 1mg/L; 1-3mg/L is poor risk; Being greater than 3mg/L is high risk.
PCT is the precursor of calcitonin.Under normal physiological conditions, PCT is by thyroid C emiocytosis, and its content is extremely low, is usually less than 0.05ng/mL.And under short inflammatory stimulus, being particularly subject under bacteriological infection or septicopyemia state, the Various Tissues except thyroid gland all can be expressed PCT and be entered blood circulation.When PCT concentration is at 0.1-0.25ng/mL, unlikely bacterial infection; PI bacterium during 0.25-0.5ng/mL; Likely bacterial infection when being greater than 0.5ng/mL, needs to use antibiotic therapy.
IL-6 is a kind of glycoprotein be made up of 184 amino acid residues, primarily of secretions such as the monocyte activated, macrophage, T and bone-marrow-derived lymphocyte, endothelial cell and fibroblasts, is the cell factor with various biological effect.IL-6 participates in systemic inflammatory response, is the important cytokine of mediation body inflammatory reaction, can promote the expression of T cell surface IL-2R, strengthens IL-l and TNF to the mitogenesis of TH cell; The synthesis of inducing acute phase reactive protein in the acute inflammatory reaction that infection or wound cause; Promote B cell proliferation, differentiation produce antibody; The cachexia that can also effectively promote TNF and IL-1 to induce.IL-6 normal reference value is 8.449-11.569pg/ml.
PCT and CRP compares, and namely can be observed PCT and constantly rise in 3-6 hour under infection stimulates, along with the PCT that increases the weight of infected constantly raises.In addition, CRP all can occur in virus and bacteriosis, and PCT level when bacterial infection disease raises, but level is lower in virus infections and nonspecific inflammation.Therefore, PCT has the features such as quick and high specificity as diagnosis index.Compared with PCT, when common infection, IL-6 level changes not obvious, and when severe infection occurs, IL-6 level obviously raises.The use in conjunction of CRP, PCT and IL-6, can judge gradient of infection and the infection type of inflammation more accurately.
Therefore, the use in conjunction of PCT, CRP and IL-6, when will be used alone than three, range of application is wider, and more convenient, diagnostic result is also more accurate, significant on clinical conditions.
Utility model content
The technical problems to be solved in the utility model is, for the deficiencies in the prior art, provides a kind of Combining diagnosis test paper assembly being easy to use, highly sensitive, efficiency is high, accuracy is good.
For solving the problems of the technologies described above, the utility model adopts following technical scheme.
A kind of Combining diagnosis test paper assembly, it includes base plate and cutting ferrule, the top layer of described base plate is provided with sample pad successively, pad, reaction film and adsorptive pads, described reaction film is provided with successively at least one quantitatively band and a quality control band, preset pitch is formed between described quantitative band and quality control band, slot is provided with in described cutting ferrule, described base plate is inserted in the slot of cutting ferrule, described cutting ferrule offers well and display window, described well and sample pad are oppositely arranged, sample is added to sample pad by described well, described display window and reaction film are oppositely arranged, the reactiveness of quantitatively band and quality control band is presented by described display window.
Preferably, the quantity of described quantitative band is three, quantitatively brings for three and is respectively equipped with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, has the PCT antibody II of different antigenic determinant with mark PCT antibody I and mark the IL-6 antibody II that IL-6 antibody I has different antigenic determinant.
Preferably, described quality control band is provided with two of mark PCT antibody I, CRP antibody I or IL-6 antibody I and resists.
Preferably, described pad is the pad being fixed with the CRP antibody I of fluorescent material, color micro-sphere or colloid gold label, PCT antibody I and IL-6 antibody I.
Preferably, described base plate is PVC fluorescence base plate.
Preferably, the quantity of described quantitative band is one, and described quantitatively bringing is provided with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, has the PCT antibody II of different antigenic determinant with mark PCT antibody I or mark the IL-6 antibody II that IL-6 antibody I has different antigenic determinant.
Preferably, the quantity of described quantitative band is two, two quantitatively bring be respectively equipped with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, have with mark PCT antibody I different antigenic determinant PCT antibody II and mark any two kinds that IL-6 antibody I has in the IL-6 antibody II of different antigenic determinant.
In Combining diagnosis test paper assembly disclosed in the utility model, can use in the slot of base plate insertion cutting ferrule, sample is added in sample pad by well simultaneously, in addition, the utility model is provided with at least one quantitatively band and a quality control band on reaction film, it can detect one or more samples as required, such as, the utility model can detect the concentration of CRP, PCT and IL-6 simultaneously, not only increase detection efficiency, also possess the advantage that CRP detects, PCT detects and IL-6 detects simultaneously.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model first embodiment.
Fig. 2 is the structural representation after base plate loads cutting ferrule.
Fig. 3 is the structural representation of the utility model second embodiment.
Fig. 4 is the structural representation of the utility model the 3rd embodiment.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in more detail.
Embodiment 1:
The present embodiment proposes a kind of Combining diagnosis test paper assembly, shown in composition graphs 1 and Fig. 2, it includes base plate 10 and cutting ferrule 15, the top layer of described base plate 10 is provided with sample pad 11 successively, pad 12, reaction film 13 and adsorptive pads 14, described reaction film 13 is provided with successively at least one quantitatively band 130 and a quality control band 131, described quantitative band 130 is positioned at the side near pad 12, preset pitch is formed between described quantitative band 130 and quality control band 131, slot is provided with in described cutting ferrule 15, described base plate 10 is inserted in the slot of cutting ferrule 15, described cutting ferrule 15 offers well 150 and display window 151, described well 150 is oppositely arranged with sample pad 11, sample is added to sample pad 11 by described well 150, described display window 151 is oppositely arranged with reaction film 13, the reactiveness of quantitatively band 130 and quality control band 131 is presented by described display window 151.
In above-mentioned Combining diagnosis test paper assembly, can use in the slot that base plate 10 inserts cutting ferrule 15, sample is added in sample pad 11 by well 150 simultaneously, the respective reaction of reaction film 13 pairs of samples can be presented intuitively by display window 151, in addition, the utility model is provided with at least one quantitatively band 130 and a quality control band 131 on reaction film 13, it can detect one or more samples as required, such as, the utility model can detect CRP simultaneously, the concentration of PCT and IL-6, not only increase detection efficiency, also possess CRP to detect simultaneously, PCT detects the advantage detected with IL-6.
Further, the quantity of described quantitative band 130 is three, and three quantitatively band 130 are respectively equipped with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, have the PCT antibody II of different antigenic determinant with mark PCT antibody I and mark the IL-6 antibody II that IL-6 antibody I has different antigenic determinant.
Preferably, quantitatively bring CRP antibody II, PCT antibody II and IL-6 antibody II, be respectively at least one for three.
In the present embodiment, described quality control band 131 is provided with two of mark PCT antibody I, CRP antibody I or IL-6 antibody I and resists.
As a kind of optimal way, described pad 12 is for being fixed with the pad of the CRP antibody I of fluorescent material, color micro-sphere or colloid gold label, PCT antibody I and IL-6 antibody I, and described base plate 10 is PVC fluorescence base plates.The present embodiment fixes the antibody of fluorescent material, color micro-sphere or colloid gold label on pad, utilizes its fluorescence or development properties, improves sensitivity and the accuracy of test paper.
Embodiment 2:
Combining diagnosis test paper assembly in the present embodiment, as shown in Figure 3, the top layer of base plate 20 is provided with sample pad 21, pad 22, reaction film 23 and adsorptive pads 24 successively, described reaction film 23 is provided with successively quantitatively band 230 and a quality control band 231, described quantitative band 230 is positioned at the side near pad 22, is formed with preset pitch between described quantitative band 230 and quality control band 231.
Further, the quantity of described quantitative band 230 is one, and described quantitative band 230 is provided with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, has the PCT antibody II of different antigenic determinant with mark PCT antibody I or mark the IL-6 antibody II that IL-6 antibody I has different antigenic determinant.
Embodiment 3:
Combining diagnosis test paper assembly in the present embodiment, as shown in Figure 4, the top layer of base plate 30 is provided with sample pad 31, pad 32, reaction film 33 and adsorptive pads 34 successively, described reaction film 33 is provided with successively quantitatively band 330 and a quality control band 331, described quantitative band 330 is positioned at the side near pad 32, is formed with preset pitch between described quantitative band 330 and quality control band 331.
Further, the quantity of described quantitative band 330 is two, two quantitatively band 330 is respectively equipped with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, have with mark PCT antibody I different antigenic determinant PCT antibody II and mark any two kinds that IL-6 antibody I has in the IL-6 antibody II of different antigenic determinant.
The utility model in actual applications, the test paper be quantitatively with containing three can be directly used to carry out Combining diagnosis, also can carry out Combining diagnosis by being quantitatively with test strips containing two and combinationally using containing a test paper be quantitatively with, also three can be combinationally used containing the quantitative test paper be with and carry out Combining diagnosis.
In Combining diagnosis test paper assembly disclosed in the utility model, base plate establishes sample pad, pad, reaction film and adsorptive pads respectively, pad is fixed with respectively mark CRP antibody I, mark PCT antibody I and mark IL-6 antibody, reaction film is equipped with quantitatively band and quality control band.CRP detects by the utility model, PCT detection detects with IL-6 and is integrated in same detection system, can detect the concentration of CRP, PCT and IL-6 simultaneously, not only increase detection efficiency, also possesses the advantage that CRP detects, PCT detection detects with IL-6 simultaneously.In addition, the utility model fixes the antibody of fluorescent material, color micro-sphere or colloid gold label on pad, utilizes its fluorescent characteristic, improves sensitivity and the accuracy of test paper.
The above is the utility model preferred embodiment, is not limited to the utility model, all make in technical scope of the present utility model amendment, equivalent to replace or improvement etc., all should be included in scope that the utility model protects.
Claims (7)
1. a Combining diagnosis test paper assembly, it is characterized in that, include base plate and cutting ferrule, the top layer of described base plate is provided with sample pad successively, pad, reaction film and adsorptive pads, described reaction film is provided with successively at least one quantitatively band and a quality control band, preset pitch is formed between described quantitative band and quality control band, slot is provided with in described cutting ferrule, described base plate is inserted in the slot of cutting ferrule, described cutting ferrule offers well and display window, described well and sample pad are oppositely arranged, sample is added to sample pad by described well, described display window and reaction film are oppositely arranged, the reactiveness of quantitatively band and quality control band is presented by described display window.
2. Combining diagnosis test paper assembly as claimed in claim 1, it is characterized in that, the quantity of described quantitative band is three, quantitatively brings for three and is respectively equipped with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, has the PCT antibody II of different antigenic determinant with mark PCT antibody I and mark the IL-6 antibody II that IL-6 antibody I has different antigenic determinant.
3. Combining diagnosis test paper assembly as claimed in claim 1, is characterized in that, described quality control band is provided with two of mark PCT antibody I, CRP antibody I or IL-6 antibody I and resists.
4. Combining diagnosis test paper assembly as claimed in claim 1, is characterized in that, described pad is the pad being fixed with the CRP antibody I of fluorescent material, color micro-sphere or colloid gold label, PCT antibody I and IL-6 antibody I.
5. Combining diagnosis test paper assembly as claimed in claim 1, it is characterized in that, described base plate is PVC fluorescence base plate.
6. Combining diagnosis test paper assembly as claimed in claim 1, it is characterized in that, the quantity of described quantitative band is one, and described quantitatively bringing is provided with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, has the PCT antibody II of different antigenic determinant with mark PCT antibody I or mark the IL-6 antibody II that IL-6 antibody I has different antigenic determinant.
7. Combining diagnosis test paper assembly as claimed in claim 1, it is characterized in that, the quantity of described quantitative band is two, two quantitatively bring be respectively equipped with from the CRP antibody II marking CRP antibody I and have different antigenic determinant, have with mark PCT antibody I different antigenic determinant PCT antibody II and mark any two kinds that IL-6 antibody I has in the IL-6 antibody II of different antigenic determinant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520839615.3U CN205080139U (en) | 2015-10-27 | 2015-10-27 | Jointly diagnose test paper subassembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520839615.3U CN205080139U (en) | 2015-10-27 | 2015-10-27 | Jointly diagnose test paper subassembly |
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| Publication Number | Publication Date |
|---|---|
| CN205080139U true CN205080139U (en) | 2016-03-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520839615.3U Ceased CN205080139U (en) | 2015-10-27 | 2015-10-27 | Jointly diagnose test paper subassembly |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107015006A (en) * | 2017-03-30 | 2017-08-04 | 北京理工大学 | A kind of cell factor immune chromatography test paper and preparation method thereof |
| CN107389925A (en) * | 2017-07-28 | 2017-11-24 | 上海交通大学医学院附属上海儿童医学中心 | The bigeminy colloidal gold immunochromatographykit kit of IL 6 and IL 10 for pyemia quick diagnosis |
-
2015
- 2015-10-27 CN CN201520839615.3U patent/CN205080139U/en not_active Ceased
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107015006A (en) * | 2017-03-30 | 2017-08-04 | 北京理工大学 | A kind of cell factor immune chromatography test paper and preparation method thereof |
| CN107389925A (en) * | 2017-07-28 | 2017-11-24 | 上海交通大学医学院附属上海儿童医学中心 | The bigeminy colloidal gold immunochromatographykit kit of IL 6 and IL 10 for pyemia quick diagnosis |
| CN107389925B (en) * | 2017-07-28 | 2019-03-29 | 上海交通大学医学院附属上海儿童医学中心 | IL-6 and IL-10 bigeminy colloidal gold immunochromatographykit kit for pyemia quick diagnosis |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| IW01 | Full invalidation of patent right | ||
| IW01 | Full invalidation of patent right |
Decision date of declaring invalidation: 20220106 Decision number of declaring invalidation: 53458 Granted publication date: 20160309 |