CN205067496U - Immunohistochemical wet box - Google Patents
Immunohistochemical wet box Download PDFInfo
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- CN205067496U CN205067496U CN201520749562.6U CN201520749562U CN205067496U CN 205067496 U CN205067496 U CN 205067496U CN 201520749562 U CN201520749562 U CN 201520749562U CN 205067496 U CN205067496 U CN 205067496U
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Abstract
The utility model discloses an immunity experiment equipment, immunohistochemical wet box includes lid, leaf grating for down pipe, hides water mechanism and box body, it includes roller bearing, clockwork spring, water proof membrane and locating lever to hide water mechanism, the box body sets up the locating hole for the cuboid form on the lateral wall of box body, be equipped with the sliding tray on the lateral wall relative with the locating hole, is equipped with the baffle in the box body, and the lower extreme when expand with the water proof membrane baffle upper end is laminated, roller bearing one end is connected with the box body lateral wall rotation at locating hole place, and the other end is connected with the box body lateral wall rotation at sliding tray place, and the lateral wall of the axis direction of roller bearing and the box body at locating hole place is perpendicular, extreme point and roller bearing fixed connection in the clockwork spring, the bonding of water proof membrane level is on clockwork spring, locating lever and the outer extreme point fixed connection of clockwork spring, the one end card of locating lever is in the sliding tray, and other end card is in the locating hole. In should wet box can be used to the relevant experiment of immunity, the problem that wet box can not the volume of controlling the evaporation of water has been solved.
Description
Technical field
The utility model belongs to immunization experiment technical field, relates to immunohistochemical wet box.
Background technology
Immunohistochemistry (Immunohistochemistry) is also known as immunocytochemistry.It is histochemical branch, and it carries out tissue and cell in-situ detection technique with the specific antibody (or antigen) of mark to organizing the distribution of endoantigen (or antibody).In every histocyte, there is antigenic material, the display of as all available in peptide class, hormone, neurotransmitter, cell factor, acceptor, surface antigen etc. immunohistochemical method, to be widely used in Preclinic and clinic scientific research thus at present.
The whole process of immunohistochemical experiment all will keep moistening environment, avoids dry plate to increase unspecific staining, so conventional wet box maintains the humidity around slice, thin piece in experiment.
At present, in laboratory, the many self-controls of technician are wet box, by filling a small amount of distilled water in centrifuge tube box, being placed on by slice, thin piece on leaf grating for down pipe, utilizing moisture to evaporate, and keep the moist environment around slice, thin piece.
China Patent Publication No. is the patent document of CN203117203U, disclose a kind of wet box, wet box is provided with top cover, the histotomy frame floated on the water surface is also provided with in wet box, histotomy frame lower surface is arranged some to the supporting walls vertical with lower surface, supporting walls is parallel to each other and size is equal, and the interval that the top magnitude setting with a pair supporting walls is equal is protruding uniformly.Lead to the histotomy frame that setting floats on the water surface in wet box, ensure that the histotomy be placed on histotomy frame is horizontal, antibody is uniformly distributed on tissue sections, reduces existing false positive or false negative, improve the accuracy of clinical test results.
But, with the homemade wet box of centrifuge tube box, because the specification of centrifuge tube mainly designs according to centrifuge tube, aperture between 0.5-5ml, point 36 lattice, 72 lattice, 100 lattice three kinds specifications.By the number in aperture, hole, the impact of ambient temperature, the speed of moisture evaporation is just different, and the air humidity of formation is just different.When covering lid, often because moisture is vaporized many, the globule can be formed in lid inside, during globule cohesion enough large, drop on again on slice, thin piece, affecting sheet protonatomic mass.Publication number antibody incubation disclosed in the patent document of CN203117203U box that wets also could not solve the vaporized many problems of moisture.So, be now badly in need of a kind of antibody incubation that can control water evaporation quantity and wet box.
Utility model content
The utility model is intended to provide immunohistochemical wet box, can not control the problem of water evaporation quantity to solve wet box at present.
Base case in this programme, immunohistochemical wet box, comprises lid, leaf grating for down pipe, Zhe Shui mechanism and box body; Zhe Shui mechanism comprises roller bearing, clockwork spring, waterproof membrane and backstay; Box body is rectangular-shaped, and the sidewall of box body arranges pilot hole, and the sidewall relative with pilot hole is provided with sliding tray, is provided with dividing plate in box body, fits in lower end when dividing plate upper end and waterproof membrane launch; The box side wall at roller bearing one end and pilot hole place is rotationally connected, and the box side wall at the other end and sliding tray place is rotationally connected, the sidewalls orthogonal of the axis direction of roller bearing and the box body at pilot hole place; In clockwork spring, end points is fixedly connected with roller bearing; Waterproof membrane level is bonded on clockwork spring; Backstay is fixedly connected with the outer end points of clockwork spring, and one end of backstay is stuck in sliding tray, and the other end is stuck in pilot hole.
The humidity in wet box can be kept after lid covers; Box body is used for filling distilled water; Leaf grating for down pipe is used for film releasing; The upper groove of leaf grating for down pipe engages with lid, the low groove of leaf grating for down pipe and Snap of box, is assembled into wet box; Roller bearing to clockwork spring deformation and can restore buffer action; Waterproof membrane can reset by clockwork spring; Backstay can slide in sliding tray, and backstay realizes location by inserting pilot hole, the developed area of fixing waterproof membrane; Waterproof membrane and dividing plate are fitted, and form the compartment of relative closure, reduce the surface area of water, thus control water evaporation quantity.
During use, box body is filled appropriate distilled water, be placed on by slice, thin piece on leaf grating for down pipe, pull backstay, waterproof membrane is drawn out, and backstay is inserted the pilot hole of suitable position, is fixed by waterproof membrane.Waterproof membrane and baffle contacts, cover part distilled water exposed area.Then carry out experimental implementation, experiment terminates bonnet upper box cover, experimentally needs, wet box is rested on experiment table or is positioned over 4 DEG C of refrigerators.
The beneficial effect of this programme is: Zhe Shui mechanism can control the evaporation capacity of moisture by the area regulating the water surface to expose, and keeps wet box to have comparatively suitable humidity, solves wet box at present and can not control the problem of water evaporation quantity.
Prioritization scheme one, as the optimization to base case, the plane that pilot hole launches along waterproof membrane arranges multiple at box side wall.Like this can the area that hides of finer adjustment damp-proof membrane, make box humidity more suitable.
Prioritization scheme two, as the optimization to prioritization scheme one, Zhe Shui mechanism symmetry arrange two.Make the partial symmetry distribution that distilled water in box exposes like this, box humidity is comparatively homogeneous.
Prioritization scheme three: as the optimization to base case, prioritization scheme two or prioritization scheme two, also comprise sponge; Sponge is positioned in box body, and the height after sponge sucks in water is lower than described dividing plate upper end.Box humidity can being ensured after sponge suctions water, when wet box needs mobile, humidity strip can not be caused because of rocking; Height after sponge sucks in water, lower than the height of pilot hole, is not stopped when can ensure that waterproof membrane is drawn out or resets.
Prioritization scheme four, as the optimization to prioritization scheme three, lid internal fixtion has moisture absorption part, and moisture absorption part is the one in thieving paper, filter paper, hospital gauze, cotton.When control lever adjusting position is improper, transpiring moisture still too much time, the globule that can gather on lid is absorbed by moisture absorption part in time, avoids being dropped on slice, thin piece.
Prioritization scheme five: as the optimization to prioritization scheme four, lid is shading lid, and box body is shading box body.Fluorescent quenching can be prevented like this.
Prioritization scheme six: as the another optimization to base case, airtightly between lid with leaf grating for down pipe is connected, airtight connection between leaf grating for down pipe and box body; All rubber strip is installed in the upper groove of leaf grating for down pipe and low groove, or lid lower end and cassette upper end are all provided with rubber strip.Wet box water vapor can be prevented like this to scatter and disappear, avoid dry plate.
Accompanying drawing explanation
Fig. 1 is the front elevation of the utility model immunohistochemical wet box embodiment one;
Fig. 2 is the schematic diagram of box body in the utility model;
Fig. 3 is the upward view of Fig. 2;
Fig. 4 is Fig. 2 vertical view;
Fig. 5 is the vertical view of leaf grating for down pipe in the utility model;
Fig. 6 is the stereographic map of lid in the utility model.
Embodiment
Below by embodiment, the utility model is described in further detail:
Reference numeral in Figure of description comprises: lid 10, thieving paper/filter paper 11, leaf grating for down pipe 20, evaporation apertures 21, upper groove 22, low groove 23, rubber strip 24, box body 30, bottom surface 31, sidewall 32, sliding tray 321, pilot hole 322, dividing plate 323, sponge 33, Zhe Shui mechanism 40, roller bearing 41, clockwork spring 42, waterproof membrane 43, backstay 44.
Embodiment one is basic as shown in accompanying drawing 1 to 6:
Immunohistochemical wet box, comprises lid 10, leaf grating for down pipe 20, Zhe Shui mechanism 40 and box body 30; Lid 10 is shading lid; Box body 30 is shading box body; Leaf grating for down pipe 20 has evaporation apertures 21, leaf grating for down pipe 20 upper end surrounding is provided with upper groove 22, and leaf grating for down pipe 20 lower end surrounding is provided with low groove 23, is all provided with rubber strip 24 in upper groove 22 and in low groove, upper groove 22 engages with the lower end of lid 10, and low groove engages with the upper end of box body 30; Zhe Shui mechanism 40 symmetry arrange two, Zhe Shui mechanism 40 comprises roller bearing 41, clockwork spring 42, waterproof membrane 43 and backstay 44; Box body 30 is rectangular-shaped, and the sidewall 32 of box body 30 arranges pilot hole 322, and the plane that pilot hole 322 launches along waterproof membrane 43 arranges multiple at box body 30 sidewall 32; The sidewall 32 relative with pilot hole 322 is provided with sliding tray 321, is provided with dividing plate 323 in box body 30, fits in lower end when dividing plate 323 upper end and waterproof membrane 43 launch; Box body 30 sidewall 32 at roller bearing 41 one end and pilot hole 322 place is rotationally connected, and box body 30 sidewall 32 at the other end and sliding tray 321 place is rotationally connected, and the axis direction of roller bearing 41 is vertical with the sidewall 32 of the box body 30 at pilot hole 322 place; In clockwork spring 42, end points is fixedly connected with roller bearing 41; Waterproof membrane 43 level is bonded on clockwork spring 42; Backstay 44 is fixedly connected with the outer end points of clockwork spring 42, and one end of backstay 44 is stuck in sliding tray 321, and the other end is stuck in pilot hole 322; Thieving paper/filter paper adhesive tape is fixed in lid 10, is placed with sponge 33 between dividing plate 323.
Roller bearing 41 and box side wall 32 are rotationally connected, and when pulling backstay 44, clockwork spring 42 is drawn out, and the waterproof membrane 43 be bonded on clockwork spring 42 is drawn out, and backstay 44 is inserted pilot hole 322, and waterproof membrane 43 covers distilled water surface, reduces evaporation area; When needs increase evaporation area, pulled out from pilot hole 322 by backstay 44, under the restoring force of clockwork spring 42, waterproof membrane 43 is rolled by roller bearing 41, backstay 44 is inserted the pilot hole 322 of correct position.Whole Zhe Shui mechanism 40 is used for regulating the surface area that breaks of distilled water, thus regulates the humidity in wet box.
Between the dividing plate 323 during use, the sponge 33 being filled distilled water being put in box body 30, the low groove 23 of leaf grating for down pipe 20 is engaged with the upper end of box body 30, install leaf grating for down pipe 20, pull-out backstay 44, backstay 44 is moved in sliding tray 321, select suitable to block position according to the size of temperature and evaporation apertures 21, then backstay 44 is inserted this place's pilot hole 322, Liang Gezhe water mechanism 40 symmetry operation.Now waterproof membrane 43 is above sponge 33, forms the space of relative closure together with dividing plate 323, reduces water evaporation quantity.When needs increase humidity, when increase evaporation area, pulled out by backstay 44 from pilot hole 322, under the restoring force of clockwork spring 42, waterproof membrane 43 is rolled by roller bearing 41, backstay 44 is inserted the pilot hole 322 of correct position.After waterproof membrane 43 is transferred to appropriate location, slice, thin piece can be placed on leaf grating for down pipe 20, carry out experimental implementation, then the lower end of lid 10 be sticked into the upper groove 22 of leaf grating for down pipe 20, namely covered lid 10.Finally experimentally need, wet box is rested on experiment table or is positioned over 4 DEG C of refrigerators.
Embodiment two: the difference of the present embodiment and embodiment one is, moisture absorption part is hospital gauze/cotton; All do not have rubber strip in the upper groove of leaf grating for down pipe and in low groove, the lower end of lid and the upper end of box body are provided with rubber strip.This embodiment can realize the effect identical with embodiment one.
Above-described is only preferred implementation of the present utility model; should be understood that; for a person skilled in the art; under the prerequisite not departing from the utility model structure; some distortion and improvement can also be made; these also should be considered as protection domain of the present utility model, and these all can not affect effect and the practical applicability of the utility model enforcement.
Claims (9)
1. immunohistochemical wet box, comprises lid, leaf grating for down pipe and box body; Leaf grating for down pipe has evaporation apertures, and the surrounding of leaf grating for down pipe upper end has upper groove, and the surrounding of leaf grating for down pipe lower end has low groove, and upper groove engages with lid, low groove and Snap of box; It is characterized in that: also comprise Zhe Shui mechanism, Zhe Shui mechanism comprises roller bearing, clockwork spring, waterproof membrane and backstay; Described box body is rectangular-shaped, and the sidewall of box body arranges pilot hole, and the sidewall relative with pilot hole is provided with sliding tray, is provided with dividing plate in box body, fits in lower end when dividing plate upper end and waterproof membrane launch; The box side wall at roller bearing one end and pilot hole place is rotationally connected, and the box side wall at the other end and sliding tray place is rotationally connected, the sidewalls orthogonal of the axis direction of roller bearing and the box body at pilot hole place; In clockwork spring, end points is fixedly connected with roller bearing; Waterproof membrane level is bonded on clockwork spring; Backstay is fixedly connected with the outer end points of clockwork spring, and one end of backstay is stuck in sliding tray, and the other end is stuck in pilot hole.
2. immunohistochemical wet box according to claim 1, is characterized in that: the plane that described pilot hole launches along waterproof membrane arranges multiple at box side wall.
3. immunohistochemical wet box according to claim 2, is characterized in that: described Zhe Shui mechanism symmetry arrange two.
4. immunohistochemical wet box according to any one of claim 1 to 3, is characterized in that: also comprise sponge; Sponge is positioned in box body, and the height after sponge sucks in water is lower than described dividing plate upper end.
5. immunohistochemical wet box according to claim 4, is characterized in that: described lid internal fixtion has moisture absorption part, and described moisture absorption part is the one in thieving paper, filter paper, hospital gauze, cotton.
6. immunohistochemical wet box according to claim 5, is characterized in that: lid is shading lid, and box body is shading box body.
7. immunohistochemical wet box according to claim 1, is characterized in that: airtightly between described lid with described leaf grating for down pipe be connected; Airtight connection between described leaf grating for down pipe and described box body.
8. immunohistochemical wet box according to claim 7, is characterized in that: described lid lower end and cassette upper end are all provided with rubber strip.
9. immunohistochemical wet box according to claim 7, is characterized in that: be all provided with rubber strip in the upper groove of described leaf grating for down pipe and low groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520749562.6U CN205067496U (en) | 2015-09-25 | 2015-09-25 | Immunohistochemical wet box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520749562.6U CN205067496U (en) | 2015-09-25 | 2015-09-25 | Immunohistochemical wet box |
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| Publication Number | Publication Date |
|---|---|
| CN205067496U true CN205067496U (en) | 2016-03-02 |
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| CN201520749562.6U Active CN205067496U (en) | 2015-09-25 | 2015-09-25 | Immunohistochemical wet box |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106338600A (en) * | 2016-08-22 | 2017-01-18 | 梁媛媛 | Safty-type immunohistochemistry wet box |
| CN112816683A (en) * | 2020-12-28 | 2021-05-18 | 广州金域医学检验中心有限公司 | Incubator for slide incubation and method of use |
-
2015
- 2015-09-25 CN CN201520749562.6U patent/CN205067496U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106338600A (en) * | 2016-08-22 | 2017-01-18 | 梁媛媛 | Safty-type immunohistochemistry wet box |
| CN112816683A (en) * | 2020-12-28 | 2021-05-18 | 广州金域医学检验中心有限公司 | Incubator for slide incubation and method of use |
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| GR01 | Patent grant |