CN205003161U - Detect many remaining aptamer test paper of diphenyl chrysanthemum ester and bromine cyanogen chrysanthemum ester - Google Patents
Detect many remaining aptamer test paper of diphenyl chrysanthemum ester and bromine cyanogen chrysanthemum ester Download PDFInfo
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- CN205003161U CN205003161U CN201520640887.0U CN201520640887U CN205003161U CN 205003161 U CN205003161 U CN 205003161U CN 201520640887 U CN201520640887 U CN 201520640887U CN 205003161 U CN205003161 U CN 205003161U
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- detection line
- chrysanthemum ester
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- decis
- biphenthrin
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Abstract
The utility model discloses a detect many remaining aptamer test paper of diphenyl chrysanthemum ester and bromine cyanogen chrysanthemum ester, comprising a base plate, on the bottom plate in proper order the order paste there is sample liquid absorption layer, aptamer fluorescence microballon combine to fill up, chromatography membrane and the pad that absorbs water, epimembranal detection line and the matter accuse line of being equipped with in proper order of chromatography, detection line include first detection line and second detection line, and first detection line is diphenyl chrysanthemum ester detection line, and the second detection line is bromine cyanogen chrysanthemum ester detection line, on first detection line and the second detection line respectively the spraying have diphenyl chrysanthemum ester aptamer complementary sequence and bromine cyanogen chrysanthemum ester aptamer complementary sequence, aptamer fluorescence microballon combine to fill up be that nanometer fluorescence microballon by both aptamer equipments of diphenyl chrysanthemum ester and bromine cyanogen chrysanthemum ester combines to fill up, aptamer fluorescence microballon combines to stack up and contains the interlinkage and have diphenyl chrysanthemum ester aptamer's nanometer fluorescence microballon and bromine cyanogen chrysanthemum ester aptamer's nanometer fluorescence microballon. The utility model discloses high, easy and simple to handle quick, accurate, with low costs, the easy popularization and application of demonstration result image of sensitivity.
Description
Technical field
The utility model relates to and a kind ofly detects Biphenthrin and the how residual fit test paper of decis, belongs to detection paper analysis technical field.
Background technology
Pyrethroid pesticide is the bionical pesticide of the 20 century 70 successful class of research and development, has stable in properties, Environmental compatibility is good, killing ability is strong, the residual effect time is long, safety coefficient advantages of higher.And along with after high-toxic organic phosphorus agricultural chemicals is prohibited from using, the pyrethroid insecticideses such as current decis are just provided with usage space widely.
Pyrethroid pesticide consumption is large, this compound is to light, thermally-stabilised, crop surface can be adhered to not easily by rain drop erosion, therefore the longevity of residure is long, entered environment not easily decomposes, easily cause environmental pollution, and the service condition of the agricultural chemicals of China is very complicated, unreasonable, overdose uses chemical pesticide for a long time, pest resistance to insecticide is strengthened year by year, and drug-fast enhancing forces again agricultural producer to continue to increase consumption and the access times of agricultural chemicals, and the concentration of agricultural chemicals is used larger and larger, the residual severe overweight of agricultural product, greatly reduces the quality of agricultural product.Since the nineties in 20th century, external a large amount of testing result shows, pyrethrin pesticide remains the important limiting factor that excessive problem has become the Chinese foreign trade of impact.The unreasonable use of agricultural chemicals causes more and more serious adverse consequences to the mankind and ecologic environment, and the pollution problem of agricultural chemicals has become the focus of global concern.
Current Biphenthrin and deltamethrin residues detect and often adopt gas chromatography and gas chromatography mass spectrometry method, because these methods need instrument costly and sample pretreatment process is complicated, are thus difficult to the needs meeting actual detection.Immune analysis method has fast, high specificity, sensitivity advantages of higher, can carry out on-site measurement, in recent years be widely used in Detecting Pesticide.U.S. B.DHammock laboratory needle been has has been researched and developed successfully pyrethroid pesticide principal immune kit of being correlated with.But Biphenthrin and decis are micromolecular compound, and itself does not have immunogenicity.Therefore, Dispersal risk needs by just becoming immunogene with macro-molecular protein is crosslinked, and process is loaded down with trivial details, and the quality of antibody affects very large by antigen.The antibody simultaneously prepared is high for the antigenic cross-reaction rate that structure is similar.
Aptamer is a kind of aglucon than antibody with more high specific, the feature of its in-vitro screening, external preparation effectively can overcome the shortcoming that antibody manufacturing cycle is long, production cost is high, technical difficulty is large, differences between batches are large, clone strain is not easily effectively preserved, and the aptamer construction cycle is short, production cost is low, the application in detection analysis field easy to use is extensively approved, therefore, aptamer has a good application prospect in the quick detection of Biphenthrin and decis.
Summary of the invention
In order to make Biphenthrin and deltamethrin residues can by quantitative detecting analysis fast, the utility model provides and a kind ofly can detect Biphenthrin and the how residual fit test paper of decis simultaneously.
The utility model adopts following technical scheme: a kind ofly detect Biphenthrin and the how residual fit test paper of decis, comprise base plate, on described base plate, order is pasted with sample liquid-adsorption layer, fit fluorescent microsphere pad, chromatographic film and adsorptive pads successively, described chromatographic film is provided with detection line and nature controlling line successively, and described detection line comprises the first detection line and the second detection line.
The first described detection line is Biphenthrin detection line, and the second detection line is decis detection line; The spacing of the first detection line and the second detection line is 0.45cm, and the spacing of the second detection line and nature controlling line is 0.45cm.
Described fit fluorescent microsphere pad is the nanometer fluorescent microspheres pad by Biphenthrin and the fit assembling of decis, and fit fluorescent microsphere pad is connected to the fit nanometer fluorescent microspheres of Biphenthrin and the fit nanometer fluorescent microspheres of decis containing chain.
Described first detection line and the second detection line are coated with the fit complementary series of Biphenthrin and the fit complementary series of decis respectively.
Described nature controlling line is biotin nature controlling line.
Described base plate is rectangular flat board.
The beneficial effects of the utility model are as follows:
1, high specificity, highly sensitive.This Biphenthrin and decis quick detection test paper by high-affinity fit based on be prepared from, minimum detectability can reach 0.01mg/kg.
2, simple to operate, detection speed is fast and accurate.Use this detection paper only need insert in sample liquid by test paper, within about 5 minutes, just can show result.Display result is more accurate, take fluorescent bands as testing result.Further research can find that in the fluorescence detector sample that adapted is special, the content of Biphenthrin/decis and the fluorescent value of detection line are inverse correlation, and in sample, Biphenthrin/decis content is higher, and the fluorescent value of detection line is lower.
3, cost is low.Fit screening process avoids animal immune, and screening process is easy, and later stage preparation only needs chemosynthesis, and do not need the steps such as cultured cell, fabrication cycle and cost are all far below antibody.And the cost of test strips is more lower than detection kit.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present utility model.
Fig. 2 is testing result is negative schematic diagram.
Fig. 3 is testing result is the positive schematic diagram of Biphenthrin.
Fig. 4 is testing result is the positive schematic diagram of decis.
Fig. 5 is testing result is the complete positive schematic diagram of Biphenthrin and decis.
Wherein: 1-base plate, 2-adsorptive pads, 3-chromatographic film, 4-is fit fluorescent microsphere pad, 5-sample liquid-adsorption layer, 6-detection line, 61-Biphenthrin detection line, 62-decis detection line, 7 nature controlling lines.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in detail.
As shown in Figure 1, a kind ofly detect Biphenthrin and the how residual fit test paper of decis, it comprises base plate 1, and order is pasted onto sample liquid-adsorption layer (5), fit fluorescent microsphere pad (4), chromatographic film (3) and the adsorptive pads (2) on base plate 1 successively; Described chromatographic film is provided with successively detection line (6) and nature controlling line (7), described detection line (6) comprises the first detection line (61) and the second detection line (62).
In the utility model, first detection line (61) is Biphenthrin detection line, second detection line (62) is decis detection line, and the first detection line (61) and the second detection line (62) are coated with the fit complementary series of Biphenthrin and the fit complementary series of decis respectively; And the first detection line (61) is 0.45cm with the spacing of the second detection line (62), and the spacing of the second detection line and described nature controlling line (7) is 0.45cm.Fit fluorescent microsphere pad (4) is the nanometer fluorescent microspheres pad by Biphenthrin and the fit assembling of decis, and fit fluorescent microsphere pad (4) is upper is connected to the fit nanometer fluorescent microspheres of Biphenthrin and the fit nanometer fluorescent microspheres of decis containing chain.Nature controlling line (7) is biotin nature controlling line.
During concrete detection, the sample liquid-adsorption layer end of test paper is inserted in fruit juice, vegetables leachate, because capillary action carries out lateral chromatography, 5-10min observations.When in sample, excess exists Biphenthrin, Biphenthrin is fit can not be combined with respective complementary sequence, then do not show fluorescent bands at the first detection line 61 place; When in sample, excess exists decis, decis is fit can not be combined with complementary series, then do not show fluorescent bands at the second place of detection line place 62.Along with the reduction of pesticide concentration, fluorescence volume raises gradually, and when there is not Biphenthrin and decis, three lines (detection line and nature controlling line) all show intense fluorescence band, reach the object of detection.
Make the fit fluorescent microsphere test paper detecting Biphenthrin and deltamethrin residues and first need to screen the fit of Biphenthrin and decis, the complementary series on synthesis detection line, prepare fluorescent microsphere in conjunction with fit; Do not need in whole preparation process to transform Biphenthrin and deltamethrin pesticide and modify, greatly simplifie preparation process compared to immune colloidal gold chromatography technology.Below the preparation method of associated materials:
1, use SELEX technology screening for Biphenthrin fit and fit for decis.
Marked in random oligonucleotide storehouse with fluorescein (FAM) in the utility model, the upstream primer that the pcr amplification FAM often taking turns screening marks carries out, and the fluorescence intensity dropping into sample and elution samples during by comparing screening judges screening effeciency.
SELEX detailed process is see patent CN203569071U.
2, the preparation of fit fluorescent microsphere pad.
(1) preparation of fluorescent microsphere: the diameter of conventional method preparation parcel fluorescent material is the silica fluorescent microballoon of 10 μm;
(2) with fluorescent microsphere mark Biphenthrin is fit and decis is fit, and be sprayed on glass fibre element film.
3, the fit test paper examinations principle of Biphenthrin and deltamethrin residues is detected.
(1) the sample liquid-adsorption layer end of test paper is inserted in fruit juice, vegetables leachate, because capillary action carries out lateral chromatography, 5min observes;
(2) fluorescent microsphere is under lamp source excitation, sends strong fluorescent bands; The fluorescent absorption wavelength connecting the fit fluorescent microsphere fit with decis of Biphenthrin is different with emission wavelength, can distinguish detection;
(3) as shown in Figures 2 to 5, detect more than bifenthrin concentration 1mg/kg, chromatographic film occurs the fluorescent bands of a nature controlling line 7 is positive; During 0.01-1mg/kg, fluorescence intensity reduces along with pesticide concentration and raises, and below 0.01mg/kg occurs that the first detection line 61 and nature controlling line 7 pairs of fluorescent bands are for negative.Detect more than decis concentration 1mg/kg, chromatographic film occurs the fluorescent bands of a nature controlling line 7 is positive; During 0.01-1mg/kg, fluorescence intensity reduces along with pesticide concentration and raises, and below 0.01mg/kg occurs that the second detection line 62 and nature controlling line 7 pairs of fluorescent bands are for negative.When two kinds of pesticide concentrations are all lower than occurring three hyperfluorescenceZeng Yongminggaoyingguang bands during 0.01mg/kg.
For person of ordinary skill in the field, along with the development of technology, the utility model design can realize by different way.Embodiment of the present utility model is not limited in embodiment described above, and can change within the scope of the claims.
The utility model does not relate to the part prior art that maybe can adopt all same as the prior art and is realized.
Claims (6)
1. one kind is detected Biphenthrin and the how residual fit test paper of decis, it is characterized in that: comprise base plate (1), on described base plate (1), order is pasted with sample liquid-adsorption layer (5), fit fluorescent microsphere pad (4), chromatographic film (3) and adsorptive pads (2) successively, described chromatographic film is provided with successively detection line (6) and nature controlling line (7), described detection line (6) comprises the first detection line (61) and the second detection line (62).
2. detection Biphenthrin according to claim 1 and the how residual fit test paper of decis, it is characterized in that: described the first detection line (61) is Biphenthrin detection line, the second detection line (62) is decis detection line; First detection line (61) is 0.45cm with the spacing of the second detection line (62), and the spacing of the second detection line and described nature controlling line (7) is 0.45cm.
3. detection Biphenthrin according to claim 1 and 2 and the how residual fit test paper of decis, it is characterized in that: described fit fluorescent microsphere pad (4) is the nanometer fluorescent microspheres pad by Biphenthrin and the fit assembling of decis, fit fluorescent microsphere pad (4) is upper is connected to the fit nanometer fluorescent microspheres of Biphenthrin and the fit nanometer fluorescent microspheres of decis containing chain.
4. detection Biphenthrin according to claim 1 and 2 and the how residual fit test paper of decis, is characterized in that: described first detection line (61) and the second detection line (62) are coated with the fit complementary series of Biphenthrin and the fit complementary series of decis respectively.
5. detection Biphenthrin according to claim 1 and 2 and the how residual fit test paper of decis, is characterized in that: described nature controlling line (7) is biotin nature controlling line.
6. detection Biphenthrin according to claim 1 and the how residual fit test paper of decis, is characterized in that: described base plate (1) is rectangular flat board.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520640887.0U CN205003161U (en) | 2015-08-24 | 2015-08-24 | Detect many remaining aptamer test paper of diphenyl chrysanthemum ester and bromine cyanogen chrysanthemum ester |
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| CN201520640887.0U CN205003161U (en) | 2015-08-24 | 2015-08-24 | Detect many remaining aptamer test paper of diphenyl chrysanthemum ester and bromine cyanogen chrysanthemum ester |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061167A (en) * | 2018-08-29 | 2018-12-21 | 郑州工程技术学院 | A kind of immune chromatography test paper detecting pyrethroid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061167A (en) * | 2018-08-29 | 2018-12-21 | 郑州工程技术学院 | A kind of immune chromatography test paper detecting pyrethroid |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160127 Termination date: 20180824 |
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| CF01 | Termination of patent right due to non-payment of annual fee |