CN205003160U - Detect fluorescence aptamer chromatography test paper of gram budweiser volume of remaining and test paper pen thereof - Google Patents
Detect fluorescence aptamer chromatography test paper of gram budweiser volume of remaining and test paper pen thereof Download PDFInfo
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- CN205003160U CN205003160U CN201520639745.2U CN201520639745U CN205003160U CN 205003160 U CN205003160 U CN 205003160U CN 201520639745 U CN201520639745 U CN 201520639745U CN 205003160 U CN205003160 U CN 205003160U
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- test paper
- fit
- carbofuran
- shell
- fluorescence
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Abstract
The utility model discloses a detect fluorescence aptamer chromatography test paper of gram budweiser volume of remaining and test paper pen thereof, the test paper includes the bottom plate, on the bottom plate in proper order the order paste there is sample liquid absorption layer, aptamer combine to fill up, NC membrane and the pad that absorbs water, the epimembranal spraying of NC has aptamer complementary sequence's detection line and the matter accuse line that is formed by the biotin, aptamer combines to stack up the spraying has the rare earth ions polymer nanoparticle who has combined gram budweiser aptamer, the test paper pen includes the test paper and is used for the cladding the shell of test paper, the inside draw -in groove that is used for installing the test paper that is equipped with of shell, the front of shell are equipped with the viewing aperture, detection line and matter accuse line position correspond on the position of viewing aperture and the test paper, the liquid -absorbant layer of test paper is located as the sense terminal the one end of shell, be equipped with the detection cap on the sense terminal. The utility model discloses sensitivity high, easy and simple to handle quick, show as a result visual in image accuracy, with low costs, easily popularize and apply on a large scale.
Description
Technical field
The utility model relates to a kind of fit chromatographic test paper of fluorescence and the test paper pen thereof that detect Determination of carbofuran amount, belongs to detection paper analysis technical field.
Background technology
Carbofuran is one of principal item of the cruel insecticides of carbamic acid, and it effectively can prevent and treat various pests on multiple kinds of crops and ornamental plant and nematode.Carbofuran is not easily degraded in acid ground, and belongs to high-toxic pesticide.The carbofuran pesticidal preparations detected in Related product containing residual must not explicitly point out in area in the U.S. etc., but at present for carbofuran agricultural chemicals, some people of China is but also stealthily using, and therefore causes the heavy losses that agricultural products in China exports.Meanwhile, the unreasonable use of agricultural chemicals causes more and more serious adverse consequences to the mankind and ecologic environment, and the pollution problem of agricultural chemicals has become the focus of global concern.
The analytical technology detecting the routine of Determination of carbofuran has gas chromatography, liquid chromatography etc.But carbofuran is a kind of heat-labile material, is easily degraded into phenols under general gas chromatography operating conditions, need to analyze after carbofuran derivatization again when therefore detecting.Now the most frequently used method, it is very large that its sensitivity is subject to the step impacts such as the purification of sample, concentrated, derivatization, in addition, these analytical approachs also need expensive exact instrument and skilled operating personnel, requirement for laboratory is very high, and can not meet the preliminary screening of batch samples far away, especially Site Detection.
Aptamer is a kind of aglucon of high specific, and its advantage is that the construction cycle is short, production cost is low, in-vitro screening, external preparation, easy to use, is extensively approved detecting the application in analysis field.Therefore, aptamer has a good application prospect in the quick detection of carbofuran.
Utility model content
In order to enable Determination of carbofuran be qualitatively detected analysis fast, the utility model provides a kind of fit chromatographic test paper of fluorescence fast highly sensitive, easy and simple to handle and test paper pen thereof.
The technical solution adopted in the utility model is as follows:
The utility model discloses a kind of fit chromatographic test paper of fluorescence detecting Determination of carbofuran amount, it comprises base plate, on described base plate, order is pasted with sample liquid-adsorption layer, fit pad, NC film and adsorptive pads, the detection line described NC film being coated with fit complementary series and the nature controlling line formed by biotin successively; Described fit pad is coated with and combines the fit rare earth ion high molecular nano-microsphere of carbofuran.
Rare earth ion high molecular nano-microsphere diameter on described fit pad is 100nm, and the coating of rare earth ion high molecular nano-microsphere contains europium ion.
It is combined with it for carrier with rare earth ion high molecular nano-microsphere that described carbofuran is fit, simultaneously carbofuran fit on be modified with streptomysin molecule.
Described base plate is rectangular flat board.
The invention also discloses a kind of fit chromatographic test paper pen of fluorescence detecting Determination of carbofuran amount, it comprises the test paper described in such scheme and the shell for coated described test paper, described enclosure is provided with the draw-in groove for installing test paper, the front of shell is provided with observation panel, and the position of described observation panel is corresponding with detection line on test paper and nature controlling line position; The liquid-adsorption layer of described test paper is positioned at one end of described shell as test side, described test side is provided with detection cap.
Use the utility model to carry out the method detected, comprise the following steps:
(1) detection zone is obtained detect cap to unplug, expose sample liquid-adsorption layer, then insert in analyte sample fluid, detect after hatching 5min;
(2) fluorescent scanning, in detection line position band fluorescence intensity and sample, the concentration of carbofuran is inversely proportional to; No matter whether have the existence of carbofuran in sample, all can there is fluorescent bands in nature controlling line, in contrast line.
The beneficial effects of the utility model are as follows:
1, the fluorescence in the utility model is fit chromatographic test paper by high-affinity fit based on be prepared from, and binding time resolved fluorometric technology detects, and its testing result degree of accuracy is high, error is little, provides convenience for Preliminary detection in enormous quantities uses.
2, test paper only need insert in sample liquid by this test paper, and within about 5 minutes, just can show result, detection speed is fast.
3, showing result more accurate, take fluorescent bands as testing result.In the fluorescence detector sample that adapted is special, carbofuran content is higher, and the fluorescent value of detection line is lower.
4, simple to operate, the needs of different levels personnel can be met preferably, be with chlamydate test paper pen to be more convenient for depositing and carrying, make this utility model have wide market outlook and larger social and economic benefits.
Accompanying drawing explanation
Fig. 1 is the structural representation of the fit chromatographic test paper of fluorescence detecting Determination of carbofuran amount in the utility model.
Fig. 2 is the testing result schematic diagram in embodiment 2.
Fig. 3 is the structural representation of the test paper pen detecting Determination of carbofuran amount in the utility model.
Wherein: 1-base plate, 2-adsorptive pads, 3-NC film, 4-is fit pad, 5-sample liquid-adsorption layer, 6-detection line, 7-nature controlling line, 8-shell, 9-detect cap, 10-observation panel.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in detail.
Embodiment 1
As shown in Figure 1, a kind of fit chromatographic test paper of fluorescence detecting Determination of carbofuran amount, it reaction zone comprised on adsorptive pads 2, NC film 3, fit pad 4 and sample liquid-adsorption layer 5, NC film 3 that order is successively pasted onto on base plate 1 is provided with the detection line 6 with complementary series and the nature controlling line 7 formed by biotin.
Described fit pad 4 to be coated with rare earth ion high analyte Nano microsphere be carrier, and carbofuran is fit, wherein, the diameter of high molecular nano-microsphere is 100nm, high molecular nano-microsphere coating contains europium ion, improve the fluorescence intensity that rare earth ion sends, therefore improve the degree of accuracy of detection.
During concrete detection, sample liquid-adsorption layer 5 end of test paper is inserted in fruit juice, vegetables leachate, because capillary action carries out lateral chromatography, observations after 5-10 minute.
Wherein, be fixed on fit pad by fit for carbofuran, being modified with streptomysin on fit can be combined by the biotin on nature controlling line, and its complementary series is as detection line, and biotin group is as nature controlling line; When in sample excessive there is carbofuran time, fitly can not to be combined with complementary series, then not show fluorescent bands at detection line place, and when existing on a small quantity, the fluorescence intensity of fluorescent bands and concentration are inverse ratio, and when there is not carbofuran, two lines all show hyperfluorescenceZeng Yongminggaoyingguang band, reach the object of detection.
Make and detect the fit fluorescent microsphere test paper of Determination of carbofuran and first need to use the fit of SELEX method screening high-affinity, prepare the complementary series on detection line, prepare fluorescent microsphere in conjunction with fit; Do not need in whole preparation process to transform carbofuran agricultural chemicals and modify, greatly simplifie preparation process compared to immune colloidal gold chromatography technology.
Embodiment 2
On the basis of embodiment 1, the present embodiment provides the examinations principle of the fit chromatographic test paper of fluorescence detecting Determination of carbofuran further:
(1) the sample liquid-adsorption layer end of test paper is inserted in fruit juice, vegetables leachate, because capillary action carries out lateral chromatography, 5-10min observes;
(2) Eu(III) ion can produce fluorescence, utilizes time-resolved fluorescence tacheometer, detects the fluorescence intensity of fluorescent bands;
(3), as shown in Figure 2, during the carbofuran concentration >50 μ g/mL of detection, NC film occurs the fluorescent bands of a nature controlling line is strong positive; Time within the scope of 0.5-50 μ g/mL, the fluorescence intensity of detection line and the concentration of carbofuran are inverse ratio, when 0.5 below μ g/mL, occur that two fluorescent bands is negative.Detectable concentration can be made into corresponding solution with carbofuran standard items, measures drawing standard curve, and corresponding detected value calculates the carbofuran concentration treated in test sample per sample.
Embodiment 3
As shown in Figure 3, on the basis of embodiment 1, the present embodiment provides a kind of fit chromatographic test paper pen of fluorescence detecting Determination of carbofuran amount further, and it comprises the shell 8 of the test paper described in embodiment 1 and coated described test paper; Described enclosure is provided with the draw-in groove for installing test paper, and the front of shell 8 is provided with observation panel 10, and the position of observation panel 10 is corresponding with detection line on test paper 6 and nature controlling line 7 position; The liquid-adsorption layer 5 of described test paper is positioned at one end of described shell 8 as test side, test side is provided with and detects cap 9.
In the present embodiment, the test paper pen with shell 8 is not only convenient to deposit and carry, and is also convenient to detection operation; And sample imbibition district has detection cap 9 to cover, take off and detect cap 9 directly immersion liquid to be measured, without the need to dripping sample with dropper, the setting of described observation panel 10 is convenient to observe testing result.
For person of ordinary skill in the field, along with the development of technology, the utility model design can realize by different way.Embodiment of the present utility model is not limited in embodiment described above, and can change within the scope of the claims.
The utility model does not relate to the part prior art that maybe can adopt all same as the prior art and is realized.
Claims (5)
1. one kind is detected the fit chromatographic test paper of fluorescence of Determination of carbofuran amount, it is characterized in that: comprise base plate (1), on described base plate (1), order is pasted with sample liquid-adsorption layer (5), fit pad (4), NC film (3) and adsorptive pads (2), the detection line (6) described NC film (3) being coated with fit complementary series and the nature controlling line (7) formed by biotin successively; Described fit pad (4) is coated with and combines the fit rare earth ion high molecular nano-microsphere of carbofuran.
2. the fit chromatographic test paper of fluorescence of detection Determination of carbofuran amount according to claim 1, it is characterized in that: the rare earth ion high molecular nano-microsphere diameter on described fit pad (4) is 100nm, and the coating of rare earth ion high molecular nano-microsphere contains europium ion.
3. the fit chromatographic test paper of fluorescence of detection Determination of carbofuran amount according to claim 1 and 2, it is characterized in that: it is combined with it for carrier with rare earth ion high molecular nano-microsphere that described carbofuran is fit, simultaneously carbofuran fit on be modified with streptomysin molecule.
4. the fit chromatographic test paper of fluorescence of detection Determination of carbofuran amount according to claim 1, is characterized in that: described base plate is rectangular flat board.
5. one kind is detected the fit chromatographic test paper pen of fluorescence of Determination of carbofuran amount, it is characterized in that: comprise the test paper described in claim 1-4 and the shell (8) for coated described test paper, described enclosure is provided with the draw-in groove for installing test paper, the front of shell is provided with observation panel (10), and the position of described observation panel (10) is corresponding with detection line on test paper (6) and nature controlling line (7) position; The liquid-adsorption layer (5) of described test paper is positioned at one end of described shell (8) as test side, described test side is provided with detects cap (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520639745.2U CN205003160U (en) | 2015-08-24 | 2015-08-24 | Detect fluorescence aptamer chromatography test paper of gram budweiser volume of remaining and test paper pen thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520639745.2U CN205003160U (en) | 2015-08-24 | 2015-08-24 | Detect fluorescence aptamer chromatography test paper of gram budweiser volume of remaining and test paper pen thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205003160U true CN205003160U (en) | 2016-01-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520639745.2U Expired - Fee Related CN205003160U (en) | 2015-08-24 | 2015-08-24 | Detect fluorescence aptamer chromatography test paper of gram budweiser volume of remaining and test paper pen thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205003160U (en) |
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2015
- 2015-08-24 CN CN201520639745.2U patent/CN205003160U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190806 Address after: Room 203, 41 Yulanyuan, 77 Lane, Guijiang Road, Xuhui District, Shanghai Patentee after: Qi Manhua Address before: No. 99 Jiangning Road, Nanjing District hirokage 211169 cities in Jiangsu Province Patentee before: Jinling Institute of Technology |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191009 Address after: Room 501, Xintonghai Building Complex Building, Chongchuan District, Nantong City, Jiangsu Province Patentee after: JIANGSU ANXIN PENSION SERVICE Co.,Ltd. Address before: Room 203, 41 Yulanyuan, 77 Lane, Guijiang Road, Xuhui District, Shanghai Patentee before: Qi Manhua |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160127 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |