CN204981898U - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
- Publication number
- CN204981898U CN204981898U CN201520700910.0U CN201520700910U CN204981898U CN 204981898 U CN204981898 U CN 204981898U CN 201520700910 U CN201520700910 U CN 201520700910U CN 204981898 U CN204981898 U CN 204981898U
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- China
- Prior art keywords
- sieve
- sieve plate
- bottle
- cell culture
- cell
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Abstract
The utility model provides a cell culture bottle, including having the bottle in cell culture chamber, the cell culture intracavity has the sieve frame that the sieve that is set up by a plurality of horizontal directions is formed, and the distance of diapire is 0.2-2mm in the sieve that is close to bottom of the bottle in the sieve frame most and the bottle, and the sieve put up in interval between the adjacent sieve be 0.2-2mm, the sieve mesh aperture of sieve is 10-50 mu m. The utility model discloses a cell culture bottle, sieve through a plurality of layers of parallel cell culture chamber in with common culture vessel separates, after adopting above -mentioned sieve, the reunion hyperplasia of cell suppresses ascending the arriving in 3D side, and trends towards the plane orientation expansion of centre gripping between the sieve, forms the tiling growth mode of plate culture, and between the sieve 0.2-2mm of interval control, simultaneously adopt the sieve on the sieve mesh aperture go on for 10-50 microns, the degree that takes place is in extremely low degree thereby final whole suppressor cell is reunited, and is unlikely to because a large amount of the reunion hinders the problems of growing.
Description
Technical field
The utility model belongs to biochemical test fixture technology field, is specifically related to a kind of Tissue Culture Flask.
Background technology
Along with the level of cell culture technology promotes gradually, in-vitro multiplication ability and the speed of T cell (T lymphocyte) have had large increase.And between cell, self have the ability of affine absorption, after division growth is faster, often inevitably there is agglomeration traits, and can increases gradually along with the degree of the propagation reunion continued.And reunite many after, inner cell, can be dead gradually and secrete death factors owing to obtaining less than enough nutriment and also without enough growing spaces, and can cause whole cell Quality Down, culture density can not get improving.
In order to slow down the situation of the reunion of the cell occurred in culturing process, the culture vessels such as Tissue Culture Flask are placed in concussion on shaking table and cultivate by general employing, promote the motion diffusibility after cell proliferation division as far as possible, but this measure can only slow down the speed of reunion a little to some extent, along with the prolongation of incubation time, the degree of reuniting remains in increase, can not suppress to reunite.
Utility model content
The object that the utility model is implemented is the above-mentioned deficiency overcoming prior art, provides a kind of Tissue Culture Flask of the reunion of restrictive cell voluntarily degree.
In order to realize above-mentioned utility model object, the technical scheme of the utility model embodiment is as follows:
A kind of Tissue Culture Flask, comprise the bottle with cell culture chamber, there is in described cell culture chamber the sieve plate frame that the sieve plate that arranged by some horizontal directions forms, be 0.2 ~ 2mm near the distance of diapire in the sieve plate of bottle body bottom and bottle in sieve plate frame, and the spacing in described sieve plate frame between adjacent sieve plate is 0.2 ~ 2mm;
The screen-aperture of described sieve plate is 10 ~ 50 μm.
Preferably, the air inlet be through to outside described bottle is also provided with bottom described cell culture chamber.
Preferably, described air inlet is provided with the valve for closing or open this air inlet.
Preferably, biofilter is provided with in described air inlet.
Preferably, described biofilter is bacterium screen sheet or the cotton ball in 22 μm, aperture.
Preferably, described sieve plate frame is provided with at least one pillar stiffener relative to the surface of bottle body bottom.
Preferably, described sieve plate frame comprises the vertically disposed union lever of at least one and described sieve plate, and described sieve plate is all fixedly connected on this union lever.
Tissue Culture Flask of the present utility model, the cell culture chamber in usual culture vessel separates by the sieve plate parallel by some layers; After adopting above-mentioned sieve plate, reunion propagation the arriving on 3D direction of cell is suppressed, and trends towards in-plane expansion clamped between sieve plate, forms the tiling growth pattern of slat chain conveyor; And spacing controls 0.2 ~ 2mm, adopts screen-aperture 10 ~ 50 microns on sieve plate to carry out between sieve plate simultaneously, the degree that final thus overall T suppression cell reunion occurs in extremely low degree, and is unlikely to because the problem of a large amount of reunion hinder growth.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is described in further detail, in accompanying drawing:
Fig. 1 is the structural representation of the utility model embodiment Tissue Culture Flask.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
The utility model example provides a kind of Tissue Culture Flask, can be shown in Figure 1, and the culturing bottle of this practicality improves based on common culturing bottle, arranges the restriction packed cell unlimited functional structure increased on three-dimensional further wherein.Specifically, the Tissue Culture Flask of this practicality comprises the bottle 10 with cell culture chamber, has a sieve plate frame 20 in this bottle 10, and sieve plate frame 20 includes some horizontally disposed sieve plates 21.Wherein, the spacing 0.2 ~ 2mm between adjacent sieve plate 21, and in sieve plate frame 20, the sieve plate (namely near the sieve plate 21 of diapire in bottle 10) of the lowest layer is also 0.2 ~ 2mm with the spacing of diapire in bottle 10; Meanwhile, 10 ~ 50 microns, the aperture of the sieve aperture on sieve plate 21.
Adopt above-mentioned Tissue Culture Flask of the present utility model, the cell culture chamber in usual culture vessel separates by the horizontal sieve plate 21 parallel by some layers.In the process of cell cultures, when not arranging any space and stopping measure, the direction of aggregation is that three-dimensional surface absorption increases gradually, after adopting above-mentioned sieve plate 21 of the present utility model, reunion after the reunion propagation of cell on 3D direction is suppressed, and trend towards planar development, form the tiling growth pattern of slat chain conveyor, thus reduce the degree of reuniting.And the spacing adopted between sieve plate 21 controls 0.2 ~ 2mm; If reason is that sieve plate 21 spacing is excessive on the one hand, cannot play and suppress the effect be in compared with low degree of reuniting, and although the spacing control of sieve plate 21 is the smaller the better in theory on the other hand, so more easily reduce the generation of reunion, but in the process of carrying out cultivating with nutrient solution, steric compression less, versus cell mobility and activity space significantly can reduce and hinder the good growth of cell; And spacing is lower, ventilation property also can be worse and worse.
Meanwhile, the screen-aperture on sieve plate 21 10 ~ 50 microns; Make aperture be in can for unicellular or comparatively oligomerization plural number cell through, avoid wide aperture and cause cell along direction, aperture extend reunion.Like this, the Tissue Culture Flask of this practicality just can make cultured cells be in low-down reunion degree, and is unlikely to occur problem agglomerating in a large number because of random three-dimensional amplification.Further, in the process implemented, be preferred for cultivating non-adherent cell, reason is in the structure design of above-mentioned sieve plate 21 as far as possible, aperture to be suitable for above-mentioned described unicellular or comparatively oligomerization plural number cell through degree; And if institute's cultured cells is adherent sexual cell, so cell can be attached to the blocking that sieve plate 21 causes sieve plate in a large number, although the end of sieve plate 21 is not closely close to seal with the inwall of culturing bottle to be connected in cultivating, and general being still placed on shaking table is cultivated in cultivating, but transmission of nutriment etc. better growing conditions can worsen, be unfavorable for the cultivation of cell on the contrary.This is also the reason adopting sieve plate 21 instead of adopt impermeable sheet glass, plastic plate etc.
Further, in the process that above-mentioned sieve plate frame 20 is implemented, culturing bottle or culture vessel are in the process of cultivating, and the molten tolerance in cultivating early stage still can be enough, and after cultivation for some time, the molten tolerance in culture vessel declines in a large number.And when there being sieve plate frame 20 to stop layer by layer, its ventilation property of locus be more in bottom culturing bottle is lower, and the part energy ingress of air of close bottleneck, so ventilation property can also keep good state; If culturing bottle volume itself is less, the quantity of sieve plate 21 is also few, and so the ventilation property of bottom can also meet the conditional request of Growth of Cells; And if culturing bottle volume ratio is comparatively large, the quantity of sieve plate 21 is more, more hinders bottom to carry out replenish air, be more unfavorable for the growth of cell.So based on this situation, the air inlet port 11 that is through to the cell cultures cavity in bottle 10 is set in the bottom of culturing bottle bottle 10, can sterile air be filled with by this air inlet port 11 in culturing bottle, thus makes up above-mentioned defect., conveniently control meanwhile, need to add valve 111 in air inlet port 11, the air be simultaneously filled with yes sterile air, to prevent infection or the pollution of miscellaneous bacteria.In order to further antipollution problem, can arrange biofilter (not shown in figure 1) in air passageways in air inlet port 11, the object of this biofilter avoids miscellaneous bacteria to enter in cell culture chamber from this air inlet port 11 to produce pollution; The embodiment of biofilter can adopt the bacteriological filter sieve in such as 22 μm, aperture (being the commercially available prod of comparative maturity) or add cotton ball and block passage; Certain cotton ball needs to be fixed in passage with the thing of net, cover and so on, to avoid directly being pushed in cell cultures cavity by air-flow in the process of inflation.
Further in the above-described embodiment, the shape of the culturing bottle adopted in upper Fig. 1 be using triangular flask as based on the improvement carried out, than being more suitable for Secondary Culture in the mode implemented, after pouring out old nutrient solution after each cultivation completes, have some cells stopped by sieve plate and be still trapped in culturing bottle, again add new nutrient solution can directly cultivate, and do not need to carry out renewed vaccination.Certainly, the culturing bottle device that intermediate cam bottle also can be extended to square column type, cylindrical etc. shape is implemented; Can also conveniently take out and be convenient to cleaning etc. at square column type and columniform culturing bottle medium-height trestle simultaneously.
Further due in the process of cell cultures, general shaking table is cultivated, and in the process of shaking table shaking or rocking, will avoid rocking of sieve plate frame 20 as far as possible, be fixed by sieve plate frame 20 as far as possible in culturing bottle; Because if sieve plate frame 20 itself is not stationary state, so sieve plate frame 20 collides frequently along with the shake of shaking table and the inner-wall surface of culturing bottle produce, to cell formed roll collision while bottle 10 also may be caused to break; And rocking of sieve plate frame 20 also significantly can increase the shearing force of nutrient solution to cell, be very large physical injury to cultured cells like this; So based on this situation, the structure of sieve plate frame 20 of the present utility model is except the horizontal sieve plate 21 with multilayer parallel, sieve plate frame 20 with the surface of the diapire of bottle 10 are provided with at least one pillar stiffener 22, after having this pillar stiffener 22, the spacing that on the one hand can realize diapire in the sieve plate of the lowest layer in sieve plate frame 20 and bottle 10 by support screen grillage 20 is 0.2 ~ 2mm; And on the other hand, technician is by the height of adjustment pillar stiffener 22 in force, makes sieve plate frame 20 just in time can form stable holding with the inwall of bottle 10, can by means of the inner-wall surface stationary screen grillage 20 of bottle 10.
And, in the process implemented, sieve plate frame 20 can also have the union lever 23 that vertically arranges (namely vertical with horizontally disposed sieve plate 21 arrange), and sieve plate 21 is all fixedly connected with this union lever 23, by this union lever 23, multiple sieve plate 21 is fixed into stable sieve plate frame 20.Wherein, union lever 23 and the mode be fixedly connected with of sieve plate 21, can adopt the conventional multiple mode of connection of mechanical field to carry out, such as screw, buckle draw-in groove etc. mode.
Tissue Culture Flask of the present utility model, the cell culture chamber in usual culture vessel separates by the sieve plate parallel by some layers; After adopting above-mentioned sieve plate, reunion propagation the arriving on 3D direction of cell is suppressed, and trends towards in-plane expansion clamped between sieve plate, forms the tiling growth pattern of slat chain conveyor; And spacing controls 0.2 ~ 2mm, adopts screen-aperture 10 ~ 50 microns on sieve plate to carry out between sieve plate simultaneously, the degree that final thus overall T suppression cell reunion occurs in extremely low degree, and is unlikely to because the problem of a large amount of reunion hinder growth.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (7)
1. a Tissue Culture Flask, comprises the bottle with cell culture chamber, it is characterized in that, has the sieve plate frame that the sieve plate that arranged by some horizontal directions forms in described cell culture chamber;
Be 0.2 ~ 2mm near the distance of diapire in the sieve plate of bottle body bottom and bottle in described sieve plate frame, and the spacing in described sieve plate frame between adjacent sieve plate is 0.2 ~ 2mm;
The screen-aperture of described sieve plate is 10 ~ 50 μm.
2. Tissue Culture Flask as claimed in claim 1, is characterized in that, be also provided with the air inlet be through to outside described bottle bottom described cell culture chamber.
3. Tissue Culture Flask as claimed in claim 2, it is characterized in that, described air inlet is provided with the valve for closing or open this air inlet.
4. Tissue Culture Flask as claimed in claim 3, is characterized in that, be provided with biofilter in described air inlet.
5. Tissue Culture Flask as claimed in claim 4, it is characterized in that, described biofilter is bacterium screen sheet or the cotton ball in 22 μm, aperture.
6. as the Tissue Culture Flask of claim as described in any one of claim 1 to 5, it is characterized in that, described sieve plate frame is provided with at least one pillar stiffener relative to the surface of diapire in bottle.
7. Tissue Culture Flask as claimed in claim 6, it is characterized in that, described sieve plate frame comprises the vertically disposed union lever of at least one and described sieve plate, and described sieve plate is all fixedly connected on this union lever.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520700910.0U CN204981898U (en) | 2015-09-11 | 2015-09-11 | Cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520700910.0U CN204981898U (en) | 2015-09-11 | 2015-09-11 | Cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204981898U true CN204981898U (en) | 2016-01-20 |
Family
ID=55116282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520700910.0U Expired - Fee Related CN204981898U (en) | 2015-09-11 | 2015-09-11 | Cell culture bottle |
Country Status (1)
| Country | Link |
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| CN (1) | CN204981898U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489534A (en) * | 2016-11-30 | 2017-03-15 | 张家港市藏联生物研究所有限公司 | A kind of mutually isolated Cordyceps militaris cultivates vessel |
-
2015
- 2015-09-11 CN CN201520700910.0U patent/CN204981898U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489534A (en) * | 2016-11-30 | 2017-03-15 | 张家港市藏联生物研究所有限公司 | A kind of mutually isolated Cordyceps militaris cultivates vessel |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 518057, room 2, building 10, Shenzhen biological incubation center, No. 302, Nanshan District hi tech, Shenzhen, Guangdong Patentee after: ISTEM REGENERATIVE MEDICINE SCI-TECH CO., LTD. Address before: 518057, Guangdong Province, Nanshan District high tech Zone, South Ring Road, No. 29, No. 02 building, international students, building No. 15 Patentee before: ISTEM REGENERATIVE MEDICINE SCI-TECH CO., LTD. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 Termination date: 20160911 |