CN204964511U - Enzyme process detects chondroitin sulfate sodium content kit - Google Patents
Enzyme process detects chondroitin sulfate sodium content kit Download PDFInfo
- Publication number
- CN204964511U CN204964511U CN201520486249.8U CN201520486249U CN204964511U CN 204964511 U CN204964511 U CN 204964511U CN 201520486249 U CN201520486249 U CN 201520486249U CN 204964511 U CN204964511 U CN 204964511U
- Authority
- CN
- China
- Prior art keywords
- kit
- solution
- chondroitin sulfate
- enzyme process
- sulfate sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 17
- 229940006423 chondroitin sulfate sodium Drugs 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 12
- 238000002474 experimental method Methods 0.000 claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 28
- 238000001914 filtration Methods 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 102000037716 Chondroitin-sulfate-ABC endolyases Human genes 0.000 claims description 5
- 108090000819 Chondroitin-sulfate-ABC endolyases Proteins 0.000 claims description 5
- 239000007983 Tris buffer Substances 0.000 claims description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 5
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 5
- 239000012498 ultrapure water Substances 0.000 claims description 5
- 238000005138 cryopreservation Methods 0.000 claims description 4
- 102000011413 Chondroitinases and Chondroitin Lyases Human genes 0.000 claims description 3
- 108010023736 Chondroitinases and Chondroitin Lyases Proteins 0.000 claims description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 235000011149 sulphuric acid Nutrition 0.000 claims description 3
- 239000001117 sulphuric acid Substances 0.000 claims description 3
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 abstract description 3
- 229920001287 Chondroitin sulfate Polymers 0.000 abstract description 2
- 229940059329 chondroitin sulfate Drugs 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract 1
- KXKPYJOVDUMHGS-OSRGNVMNSA-N chondroitin sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](OS(O)(=O)=O)[C@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](C(O)=O)O1 KXKPYJOVDUMHGS-OSRGNVMNSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses an enzyme process detects chondroitin sulfate sodium content kit, include each one kit A and B, the A box is for testing consumptive material and solution A, solution B, and room temperature long -term storage, B box are liquid chondroitin sulfate enzyme solution, need - 20 DEG C or - 80 DEG C of saves. The utility model discloses an enzyme process detects chondroitin sulfate sodium content kit, structural design is simple, saves a large amount of experiment time of preparation of the experimenter, and the test is convenient, high -efficient reliable fast, and the test result is stable.
Description
Technical field
The utility model relates to a kind of enzyme process and detects content of chondroitin sulfate sodium kit.
Background technology
In heparin quality assessment and the correlative study of chondroitin sulfate structure, the assay of chondroitin sulfate is an important indicator, it is the method detected in conjunction with HPLC with chondroitinase ABC enzymolysis in existing Chinese Pharmacopoeia, complex operation step, required reagent, consumptive material kind are more, need to expend a large amount of time and efforts of experimenter, and easily data error is large, experimental repeatability is poor because manual operation reason causes, does not also have complete enzyme process to detect sodium chondroitin sulfate kit at present.
Utility model content
Fundamental purpose of the present utility model is that simplifying enzyme process detects content of chondroitin sulfate sodium detection method of operating, and provides a kind of enzyme process to detect content of chondroitin sulfate sodium kit.
For achieving the above object, the technical solution of the utility model provides a kind of enzyme process to detect content of chondroitin sulfate sodium kit, comprise each one of kit A and B, A box is experiment consumptive material and solution A, solution B, room temperature is preserved for a long time, B box is fluid sulphuric acid chondroitinase solution, needs-20 DEG C or-80 DEG C of preservations.
Further, described kit A, is divided into the left and right sides, and left side is experiment consumptive material, and right side is solution A, solution B and Reagent Tube.
Further, left side, described experiment consumptive material, comprises cursory, 0 ~ 20ml centrifuge tube, syringe, filtering head.
Further, right side, described solution A, solution B, comprise 0 ~ 100ml ultrapure water, 0 ~ 50ml TRIS buffer.
Further, right side, described Reagent Tube, comprises 0 ~ 2ml centrifuge tube, 0 ~ 5ml cryopreservation tube.
Further, described kit B is chondroitinase ABC solution.
Compared with prior art, the utility model has the advantage of: structural design is simple, save the Preparatory work of experiment time that experimenter is a large amount of, test quick and convenient, high efficient and reliable, test result is stablized.
Accompanying drawing explanation
Fig. 1, Fig. 2 are that a kind of enzyme process of the utility model detects content of chondroitin sulfate sodium kit A box, B box structural representation.
Wherein Fig. 1: bag 1 is cursory, bag 20-20ml centrifuge tube, bag 3 large model syringes, wrap 4 ting model syringes, bag 5 large model filtering heads, wrap 6 ting model filtering heads, A is ultrapure water, B is TRIS buffer, and 1 is 20 0-2ml centrifuge tubes, and 2 is 20 0-5ml cryopreservation tubes.Wherein Fig. 2: 3 is chondroitinase ABC.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples for illustration of the utility model, but are not used for limiting scope of the present utility model.
A kind of enzyme process of the present embodiment detects the structure of content of chondroitin sulfate sodium kit as shown in Figure 1 and Figure 2, comprise each one of kit A and B, A box is experiment consumptive material and solution A, solution B, and room temperature is preserved for a long time, B box is fluid sulphuric acid chondroitinase solution, needs-20 DEG C or-80 DEG C of preservations.
Described kit A, is divided into the left and right sides, and left side is experiment consumptive material, and right side is solution A, solution B, Reagent Tube.
On the left of kit A, described experiment consumptive material, comprises cursory, 0 ~ 20ml centrifuge tube, syringe, filtering head.
On the right side of kit A, described solution A, solution B, comprise 0 ~ 100ml ultrapure water, 0 ~ 50ml TRIS buffer.
On the right side of kit A, described Reagent Tube, comprises 0 ~ 2ml centrifuge tube, 0 ~ 5ml cryopreservation tube.
Described kit B is chondroitinase ABC solution.
Method of operating:
Step 1 uses A box 0-20ml centrifuge tube bag 2, precision weighing national drug standards material sodium chondroitin sulfate or test sample.
Step 2 uses A box ultrapure water solution A to dissolve and is settled to 0-10ml.
Step 3 uses A box large model syringe bag 3, large model filtering head bag 5 is filtered to 0-20ml centrifuge tube bag 2.
Step 4 pipettes the 0-2ml centrifuge tube of filtering solution 0-100ul to A box 1, does Duplicate Samples.
Step 5 adds TRIS buffer solution B, does Duplicate Samples.
Step 6 uses B box 1 pipe ABC enzyme all to add in step 5 centrifuge tube, does Duplicate Samples.
Step 737 DEG C water-bath 1h.
Step 8100 DEG C heating 5min.
Step 9 is cooled to room temperature, centrifugal.
Step 10 uses A box syringe bag 4, ting model filtering head bag 6 filtering reacting liquid.
Step 11HPLC analyzes and records collection of illustrative plates.
The utility model has the advantage of: structural design is simple, save the Preparatory work of experiment time that experimenter is a large amount of, test quick and convenient, high efficient and reliable, test result is stablized.
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the utility model know-why; can also make some improvement, these improvement also should be considered as protection domain of the present utility model.
Claims (4)
1. enzyme process detects a content of chondroitin sulfate sodium kit, it is characterized in that, comprises each one of kit A and B, and A box is experiment consumptive material and solution A, solution B, and room temperature is preserved for a long time, and B box is fluid sulphuric acid chondroitinase solution, needs-20 DEG C or-80 DEG C of preservations; Test consumptive material in described kit A and comprise cursory, 0 ~ 20ml centrifuge tube, syringe, filtering head; In described kit A, solution A is 0 ~ 100ml ultrapure water, solution B is 0 ~ 50ml TRIS buffer.
2. enzyme process according to claim 1 detects content of chondroitin sulfate sodium kit, and it is characterized in that, described kit A, is divided into the left and right sides, and left side is experiment consumptive material, and right side is solution A, solution B and Reagent Tube.
3. enzyme process according to claim 2 detects content of chondroitin sulfate sodium kit, and it is characterized in that, on the right side of kit A, described Reagent Tube, comprises 0 ~ 2ml centrifuge tube, 0 ~ 5ml cryopreservation tube.
4. enzyme process according to claim 1 detects content of chondroitin sulfate sodium kit, and it is characterized in that, be chondroitinase ABC solution in described kit B.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520486249.8U CN204964511U (en) | 2015-07-08 | 2015-07-08 | Enzyme process detects chondroitin sulfate sodium content kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520486249.8U CN204964511U (en) | 2015-07-08 | 2015-07-08 | Enzyme process detects chondroitin sulfate sodium content kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204964511U true CN204964511U (en) | 2016-01-13 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520486249.8U Active CN204964511U (en) | 2015-07-08 | 2015-07-08 | Enzyme process detects chondroitin sulfate sodium content kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204964511U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107460179A (en) * | 2017-09-22 | 2017-12-12 | 青岛农业大学 | A kind of polysaccharide degrading enzyme and its encoding gene and application |
-
2015
- 2015-07-08 CN CN201520486249.8U patent/CN204964511U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107460179A (en) * | 2017-09-22 | 2017-12-12 | 青岛农业大学 | A kind of polysaccharide degrading enzyme and its encoding gene and application |
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Address after: Room A417, 4th Floor, No. 5 Pioneer Road, Haidian District, Beijing 100085 Patentee after: BEIJING BIOKANGTAI MEDICINE SCIENCE & TECHNOLOGY CO.,LTD. Address before: Room B402, 4th Floor, No. 5 Kaifeng Road, Haidian District, Beijing 100085 Patentee before: BEIJING BIOKANGTAI MEDICINE SCIENCE & TECHNOLOGY CO.,LTD. |
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Addressee: Zhou Qianqian Document name: payment instructions |
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