CN204945040U - Fluorescence excitation light source Apparatus and system and fluorescence microimaging systems - Google Patents

Fluorescence excitation light source Apparatus and system and fluorescence microimaging systems Download PDF

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Publication number
CN204945040U
CN204945040U CN201520556512.6U CN201520556512U CN204945040U CN 204945040 U CN204945040 U CN 204945040U CN 201520556512 U CN201520556512 U CN 201520556512U CN 204945040 U CN204945040 U CN 204945040U
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source apparatus
module
optically focused
fluorescent exciting
monochromatic led
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夏浩涵
张莹莹
罗浦文
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Shanghai Ruiyu Biotech Co Ltd
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Shanghai Ruiyu Biotech Co Ltd
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Abstract

The utility model discloses a kind of fluorescence excitation light source Apparatus and system and fluorescence microimaging systems, comprising: adjustable fixed mount; And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter; Wherein, described optically focused module is arranged between described monochromatic LED module and bandpass filter, or described bandpass filter is arranged between described monochromatic LED module and optically focused module, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.The technical scheme that the utility model provides, fluorescent exciting source apparatus have the response time short, consume energy low, cost is low, long service life, volume are little, emitting brightness is high, be beneficial to the experiment of long-term living cells, the fluorescence excitation light source unicity advantages of higher that sends.

Description

Fluorescence excitation light source Apparatus and system and fluorescence microimaging systems
This application claims and submitted on 07 01st, 2015 the right of priority that Patent Office of the People's Republic of China, application number are 201510376530.0, denomination of invention is the Chinese patent application of " integrated form fluorescent exciting source apparatus " to, its full content combines in this application by reference.
Technical field
The utility model relates to fluorescence analysis field, more specifically, relates to a kind of fluorescence excitation light source Apparatus and system and fluorescence microimaging systems.
Background technology
At present, in fluorescence microimaging systems, its fluorescent exciting source apparatus generally adopts all band such as mercury lamp and xenon lamp light source as fluorescence excitation light source, makes existing fluorescent exciting source apparatus have the shortcoming that energy consumption is high, cost is high, the life-span is short.Especially, xenon lamp and mercury lamp all can as white light sources, although the light of a series of wavelength from ultraviolet near infrared can be provided, but they have different excitation spectrums, although this high excitation energy at peak value can be guaranteed to produce bright fluorescence signal, but phototoxicity is very strong, is unfavorable for that long-term living cells is tested.
Utility model content
In view of this, the utility model provides a kind of fluorescence excitation light source Apparatus and system and fluorescence microimaging systems, adopt monochromatic LED module as fluorescence excitation light source, additionally by the cooperation of optically focused module and bandpass filter, make fluorescent exciting source apparatus have the response time short, consume energy low, cost is low, long service life, volume are little, emitting brightness is high, be beneficial to the advantages such as long-term living cells experiment.
For achieving the above object, the technical scheme that provides of the utility model is as follows:
A kind of fluorescent exciting source apparatus, comprising:
Adjustable fixed mount;
And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter;
Wherein, described optically focused module is arranged between described monochromatic LED module and bandpass filter, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.
Preferably, described optically focused module comprises collector lens or lens combination.
Preferably, described monochromatic LED module comprises at least one monochromatic LED lamp pearl.
Preferably, described adjustable fixed mount is sealing adjustable fixed mount, and described monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in described adjustable fixed mount.
And a kind of fluorescent exciting source apparatus, comprising:
Adjustable fixed mount;
And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter;
Wherein, described bandpass filter is arranged between described monochromatic LED module and optically focused module, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.
Preferably, described optically focused module comprises collector lens or lens combination.
Preferably, described monochromatic LED module comprises at least one monochromatic LED lamp pearl.
Preferably, described adjustable fixed mount is sealing adjustable fixed mount, and described monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in described adjustable fixed mount.
Accordingly, the utility model additionally provides a kind of fluorescent exciting origin system, and described fluorescent exciting origin system comprises at least one fluorescent exciting source apparatus, and wherein, described fluorescent exciting source apparatus is above-mentioned fluorescent exciting source apparatus.
Accordingly, the utility model additionally provides a kind of fluorescence microimaging systems, and described fluorescence microimaging systems comprises fluorescent exciting origin system, and wherein, described fluorescent exciting origin system is above-mentioned fluorescent exciting origin system.
Compared to prior art, the technical scheme that the utility model provides at least has the following advantages:
The utility model provides a kind of fluorescence excitation light source Apparatus and system and fluorescence microimaging systems, comprising: adjustable fixed mount; And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter; Wherein, described optically focused module is arranged between described monochromatic LED module and bandpass filter, or described bandpass filter is arranged between described monochromatic LED module and optically focused module, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.
As shown in the above, the technical scheme that the utility model provides, adopt but LED module as fluorescence excitation light source, and because LED has, the response time is short, consume energy low, the feature such as cost is low, long service life, volume are little, and fluorescent exciting source apparatus is had, and the response time is short, consume energy low, the advantage such as cost is low, long service life, volume are little.And, because not only the decay of LED light fast but also accurate, under long-term living cells test, can phototoxicity be greatly reduced, make fluorescent exciting source apparatus be beneficial to the experiment of long-term living cells.
In addition, optically focused module is arranged at the front end of monochromatic LED module, not only make the emitting brightness of fluorescent exciting source apparatus high, and can also by the distance between adjustable fixed mount adjustment optically focused module and monochromatic LED module, to regulate the bright dipping spot size of fluorescent exciting source apparatus, and then the aggregation extent of the excitation source energy of the bright dipping of adjustment fluorescent exciting source apparatus.
In addition, bandpass filter is arranged at the front end of monochromatic LED module, goes out optical wavelength range with what retrain fluorescent exciting source apparatus, improve the unicity of the fluorescence excitation light source sent of fluorescent exciting source apparatus further.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only embodiment of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to the accompanying drawing provided.
The tangent plane structural representation of a kind of fluorescent exciting source apparatus that Fig. 1 provides for the application one embodiment;
The tangent plane structural representation of a kind of fluorescent exciting source apparatus that Fig. 2 provides for another embodiment of the application.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
As described in background, existing in fluorescence microimaging systems, its fluorescent exciting source apparatus generally adopts all band such as mercury lamp and xenon lamp light source as fluorescence excitation light source, makes existing fluorescent exciting source apparatus have the shortcoming that energy consumption is high, cost is high, the life-span is short.Especially, xenon lamp and mercury lamp all can as white light sources, although the light of a series of wavelength from ultraviolet near infrared can be provided, but they have different excitation spectrums, although this high excitation energy at peak value can be guaranteed to produce bright fluorescence signal, but phototoxicity is very strong, is unfavorable for that long-term living cells is tested.
Based on this, the embodiment of the present application provides a kind of fluorescent exciting source apparatus, adopt monochromatic LED (LightEmittingDiode, light emitting diode) module is as fluorescence excitation light source, additionally by the cooperation of optically focused module and bandpass filter, make fluorescent exciting source apparatus have the response time short, consume energy low, cost is low, long service life, volume are little, emitting brightness is high, be beneficial to the advantages such as long-term living cells experiment.Shown in concrete composition graphs 1 and Fig. 2, the fluorescent exciting source apparatus that the application provides is described in detail.
Shown in figure 1, be the tangent plane structural representation of a kind of fluorescent exciting source apparatus that the application one embodiment provides, wherein, fluorescent exciting source apparatus, comprising:
Adjustable fixed mount 100;
And, be fixed on the monochromatic LED module 200 in adjustable fixed mount 100, optically focused module 300 and bandpass filter 400;
Wherein, optically focused module 300 is arranged between monochromatic LED module 200 and bandpass filter 400, and adjustable fixed mount 100 can adjust the distance between monochromatic LED module 200 and optically focused module 300.
As shown in the above, the technical scheme that the embodiment of the present application provides, adopt but LED module as fluorescence excitation light source, and because LED has the features such as the response time is short, cost is low, long service life, volume are little, make fluorescent exciting source apparatus have the advantages such as the response time is short, cost is low, long service life, volume are little.And, because not only the decay of LED light fast but also accurate, under long-term living cells test, can phototoxicity be greatly reduced, make fluorescent exciting source apparatus be beneficial to the experiment of long-term living cells.And, because LED only needs the power of 3W can realize the fluorescent exciting of the identical wave band provided with mercury lamp, xenon lamp etc., the power of 50W to 100W is needed compared to mercury lamp, and xenon lamp needs the situation of the power of 100W, LED has the low feature of power consumption, makes fluorescent exciting source apparatus greatly reduce power consumption.
In addition, optically focused module is arranged at the front end of monochromatic LED module, not only make the emitting brightness of fluorescent exciting source apparatus high, and can also by the distance between adjustable fixed mount adjustment optically focused module and monochromatic LED module, to regulate the bright dipping spot size of fluorescent exciting source apparatus, and then the aggregation extent of the excitation source energy of the bright dipping of adjustment fluorescent exciting source apparatus.
In addition, bandpass filter is arranged at the front end of monochromatic LED module, goes out optical wavelength range with what retrain fluorescent exciting source apparatus, improve the unicity of the fluorescence excitation light source sent of fluorescent exciting source apparatus further.Such as, the peak wavelength scope of blue led module is 470 ± 15nm (comprising endpoint value), and the bandpass filter corresponding with it can be the light of 470 ± 15nm (comprising endpoint value) by wavelength coverage, therefore, the wavelength of the fluorescence excitation light source that fluorescent exciting source apparatus can be made to send by bandpass filter concentrates between 465nm ~ 485nm (comprising endpoint value), improves the unicity of the fluorescence excitation light source sent of fluorescent exciting source apparatus further.
Wherein, the optically focused module that the embodiment of the present application provides includes but not limited to collector lens or lens combination, namely, the optically focused module that the embodiment of the present application provides can for an independent collector lens, or, can also be the lens combination with light-focusing function of multiple lens composition, concrete restriction not done to this application, needs to carry out specific design according to actual needs.And the application is not restricted equally for the material of lens in collector lens and lens combination.
In addition, the monochromatic LED module that the embodiment of the present application provides comprises at least one monochromatic LED lamp pearl, and for the quantity of monochromatic LED lamp pearl in LED module, need to carry out specific design according to practical application, the application does not do concrete restriction.
Further, the waste of the fluorescence excitation light source that LED module provides in fluorescent exciting source apparatus, the adjustable fixed mount that the embodiment of the present application provides is sealing adjustable fixed mount, and monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in adjustable fixed mount.
Namely, monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in adjustable fixed mount, ensure that the fluorescence excitation light source that monochromatic LED module sends all is incident to optically focused module, fluorescence excitation light source then through optically focused module is all incident to bandpass filter, then by rear outgoing after filtering, avoid the waste before the bright dipping of fluorescent exciting source apparatus, what improve fluorescent exciting source apparatus goes out light intensity.
It should be noted that, the application does not do concrete restriction for the shape of adjustable fixed mount, in addition, the application does not do concrete restriction for the mode of the distance between adjustable fixed mount adjustment monochromatic LED module and collector lens, all needs to carry out specific design according to practical application.
The fluorescent exciting source apparatus that the above embodiments of the present application provide, its optically focused module is arranged between monochromatic LED module and bandpass filter; In addition, in another embodiment of the application, optically focused module can also be arranged at the side that bandpass filter deviates from monochromatic LED module.Concrete, shown in figure 2, be the tangent plane structural representation of a kind of fluorescent exciting source apparatus that another embodiment of the application provides, wherein, fluorescent exciting source apparatus, comprising:
Adjustable fixed mount 100;
And, be fixed on the monochromatic LED module 200 in adjustable fixed mount 100, optically focused module 300 and bandpass filter 400;
Wherein, bandpass filter 400 is arranged between monochromatic LED module 200 and optically focused module 300, and adjustable fixed mount 100 can adjust the distance between monochromatic LED module 200 and optically focused module 300.
As shown in the above, the technical scheme that the embodiment of the present application provides, adopt but LED module as fluorescence excitation light source, and because LED has the features such as the response time is short, cost is low, long service life, volume are little, make fluorescent exciting source apparatus have the advantages such as the response time is short, cost is low, long service life, volume are little.And, because not only the decay of LED light fast but also accurate, under long-term living cells test, can phototoxicity be greatly reduced, make fluorescent exciting source apparatus be beneficial to the experiment of long-term living cells.And, because LED only needs the power of 3W can realize the fluorescent exciting of the identical wave band provided with mercury lamp, xenon lamp etc., the power of 50W to 100W is needed compared to mercury lamp, and xenon lamp needs the situation of the power of 100W, LED has the low feature of power consumption, makes fluorescent exciting source apparatus greatly reduce power consumption.
In addition, optically focused module is arranged at the front end of monochromatic LED module, not only make the emitting brightness of fluorescent exciting source apparatus high, and can also by the distance between adjustable fixed mount adjustment optically focused module and monochromatic LED module, to regulate the bright dipping spot size of fluorescent exciting source apparatus, and then the aggregation extent of the excitation source energy of the bright dipping of adjustment fluorescent exciting source apparatus.
In addition, bandpass filter is arranged at the front end of monochromatic LED module, goes out optical wavelength range with what retrain fluorescent exciting source apparatus, improve the unicity of the fluorescence excitation light source sent of fluorescent exciting source apparatus further.Such as, the peak wavelength scope of blue led module is 470 ± 15nm (comprising endpoint value), and the bandpass filter corresponding with it can be the light of 470 ± 15nm (comprising endpoint value) by wavelength coverage, therefore, the wavelength of the fluorescence excitation light source that fluorescent exciting source apparatus can be made to send by bandpass filter concentrates between 465nm ~ 485nm (comprising endpoint value), improves the unicity of the fluorescence excitation light source sent of fluorescent exciting source apparatus further.
Wherein, the optically focused module that the embodiment of the present application provides includes but not limited to collector lens or lens combination, namely, the optically focused module that the embodiment of the present application provides can for an independent collector lens, or, can also be the lens combination with light-focusing function of multiple lens composition, concrete restriction not done to this application, needs to carry out specific design according to actual needs.And the application is not restricted equally for the material of lens in collector lens and lens combination.
In addition, the monochromatic LED module that the embodiment of the present application provides comprises at least one monochromatic LED lamp pearl, and for the quantity of monochromatic LED lamp pearl in LED module, need to carry out specific design according to practical application, the application does not do concrete restriction.
Further, the waste of the fluorescence excitation light source that LED module provides in fluorescent exciting source apparatus, the adjustable fixed mount that the embodiment of the present application provides is sealing adjustable fixed mount, and monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in adjustable fixed mount.
Namely, monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in adjustable fixed mount, ensure that the fluorescence excitation light source that monochromatic LED module sends all is incident to optically focused module, fluorescence excitation light source then through optically focused module is all incident to bandpass filter, then by rear outgoing after filtering, avoid the waste before the bright dipping of fluorescent exciting source apparatus, what improve fluorescent exciting source apparatus goes out light intensity.
It should be noted that, the application does not do concrete restriction for the shape of adjustable fixed mount, in addition, the application does not do concrete restriction for the mode of the distance between adjustable fixed mount adjustment monochromatic LED module and collector lens, all needs to carry out specific design according to practical application.
Accordingly, the embodiment of the present application additionally provides a kind of fluorescent exciting origin system, and fluorescent exciting origin system comprises at least one fluorescent exciting source apparatus, wherein, and the fluorescent exciting source apparatus that fluorescent exciting source apparatus provides for above-mentioned any embodiment.
Accordingly, the embodiment of the present application additionally provides a kind of fluorescence microimaging systems, and fluorescence microimaging systems comprises fluorescent exciting origin system, wherein, and the fluorescent exciting origin system that fluorescent exciting origin system provides for above-mentioned any embodiment.
The embodiment of the present application provides a kind of fluorescence excitation light source Apparatus and system and fluorescence microimaging systems, comprising: adjustable fixed mount; And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter; Wherein, described optically focused module is arranged between described monochromatic LED module and bandpass filter, or described bandpass filter is arranged between described monochromatic LED module and optically focused module, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.
As shown in the above, the technical scheme that the embodiment of the present application provides, adopt but LED module as fluorescence excitation light source, and because LED has, the response time is short, consume energy low, the feature such as cost is low, long service life, volume are little, and fluorescent exciting source apparatus is had, and the response time is short, consume energy low, the advantage such as cost is low, long service life, volume are little.And, because not only the decay of LED light fast but also accurate, under long-term living cells test, can phototoxicity be greatly reduced, make fluorescent exciting source apparatus be beneficial to the experiment of long-term living cells.
In addition, optically focused module is arranged at the front end of monochromatic LED module, not only make the emitting brightness of fluorescent exciting source apparatus high, and can also by the distance between adjustable fixed mount adjustment optically focused module and monochromatic LED module, to regulate the bright dipping spot size of fluorescent exciting source apparatus, and then the aggregation extent of the excitation source energy of the bright dipping of adjustment fluorescent exciting source apparatus.
In addition, bandpass filter is arranged at the front end of monochromatic LED module, goes out optical wavelength range with what retrain fluorescent exciting source apparatus, improve the unicity of the excitation source of the bright dipping of fluorescent exciting source apparatus further.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the utility model.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein when not departing from spirit or scope of the present utility model, can realize in other embodiments.Therefore, the utility model can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.

Claims (10)

1. a fluorescent exciting source apparatus, is characterized in that, comprising:
Adjustable fixed mount;
And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter;
Wherein, described optically focused module is arranged between described monochromatic LED module and bandpass filter, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.
2. fluorescent exciting source apparatus according to claim 1, is characterized in that, described optically focused module comprises collector lens or lens combination.
3. fluorescent exciting source apparatus according to claim 1, is characterized in that, described monochromatic LED module comprises at least one monochromatic LED lamp pearl.
4. fluorescent exciting source apparatus according to claim 1, is characterized in that, described adjustable fixed mount is sealing adjustable fixed mount, and described monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in described adjustable fixed mount.
5. a fluorescent exciting source apparatus, is characterized in that, comprising:
Adjustable fixed mount;
And, be fixed on the monochromatic LED module in described adjustable fixed mount, optically focused module and bandpass filter;
Wherein, described bandpass filter is arranged between described monochromatic LED module and optically focused module, and described adjustable fixed mount can adjust the distance between described monochromatic LED module and described optically focused module.
6. fluorescent exciting source apparatus according to claim 5, is characterized in that, described optically focused module comprises collector lens or lens combination.
7. fluorescent exciting source apparatus according to claim 5, is characterized in that, described monochromatic LED module comprises at least one monochromatic LED lamp pearl.
8. fluorescent exciting source apparatus according to claim 5, is characterized in that, described adjustable fixed mount is sealing adjustable fixed mount, and described monochromatic LED module, optically focused module and bandpass filter are all sealingly fastened in described adjustable fixed mount.
9. a fluorescent exciting origin system, is characterized in that, described fluorescent exciting origin system comprises at least one fluorescent exciting source apparatus, and wherein, described fluorescent exciting source apparatus is the fluorescent exciting source apparatus described in claim 1 ~ 8 any one.
10. a fluorescence microimaging systems, is characterized in that, described fluorescence microimaging systems comprises fluorescent exciting origin system, and wherein, described fluorescent exciting origin system is fluorescent exciting origin system according to claim 9.
CN201520556512.6U 2015-07-28 2015-07-28 Fluorescence excitation light source Apparatus and system and fluorescence microimaging systems Active CN204945040U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104949953A (en) * 2015-07-01 2015-09-30 上海睿钰生物科技有限公司 Fluorescence excitation light source device and system, as well as fluorescence microscopy imaging system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104949953A (en) * 2015-07-01 2015-09-30 上海睿钰生物科技有限公司 Fluorescence excitation light source device and system, as well as fluorescence microscopy imaging system
WO2017000702A1 (en) * 2015-07-01 2017-01-05 上海睿钰生物科技有限公司 Fluorescence excitation light source device and system, and fluorescence microscopy imaging system

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