CN204945039U - Aflatoxin ultraviolet fluorescent detecting device - Google Patents

Aflatoxin ultraviolet fluorescent detecting device Download PDF

Info

Publication number
CN204945039U
CN204945039U CN201520535444.5U CN201520535444U CN204945039U CN 204945039 U CN204945039 U CN 204945039U CN 201520535444 U CN201520535444 U CN 201520535444U CN 204945039 U CN204945039 U CN 204945039U
Authority
CN
China
Prior art keywords
fluorescence
aflatoxin
detecting device
condenser
grating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201520535444.5U
Other languages
Chinese (zh)
Inventor
林晓燕
曹赵云
陈铭学
牟仁祥
朱智伟
吴莉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China National Rice Research Institute
Original Assignee
China National Rice Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China National Rice Research Institute filed Critical China National Rice Research Institute
Priority to CN201520535444.5U priority Critical patent/CN204945039U/en
Application granted granted Critical
Publication of CN204945039U publication Critical patent/CN204945039U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The utility model relates to a kind of detecting instrument, especially a kind of aflatoxin ultraviolet fluorescent detecting device.Grating is arranged on directly over culture tank, condenser is fixed on directly over grating, fluorescence spectrophotometer mirror is arranged on directly over condenser, fluorescence display plate is arranged on the top of fluorescence spectrophotometer mirror, described fluorescence display plate is fluorescence projection plate, Ultraviolet radiation light source is a luminescence-utraviolet device, and the head of illuminator is corresponding with culture tank to be arranged.Said structure when in use, nutrient culture media is placed on mounting table, then regulate Ultraviolet radiation light source to irradiate nutrient culture media, make it that fluorescence occur, by carrying out optically focused by condenser after the screening of grating, adding the intensity of high light, then carrying out light splitting by spectroscope and projecting on fluorescence display plate, just significantly can observe the color of fluorescence, the situation of aflatoxin can be judged by fluorescence.

Description

Aflatoxin ultraviolet fluorescent detecting device
Technical field
The utility model relates to a kind of detecting instrument, especially a kind of aflatoxin ultraviolet fluorescent detecting device.
Background technology
Aflatoxin (Aflatoxins, AFT) is the secondary metabolite of fungi, is mainly produced by bacterial strains such as aspergillus flavus (A.flavus), aspergillus parasiticuses (A.parasiticus); When grain fails to dry in time and stored not at that time, often easily polluted by aspergillus flavus or aspergillus parasiticus and produce this toxoid.Aflatoxin is the hepatotoxin that a kind of toxicity is very strong, the acute of liver or chronic lesion can be caused, except the liver of infringement body, aflatoxin also can cause serious harm to other Various Tissues organs such as kidneys, and more seriously aflatoxin has been proved and has had carcinogenic, teratogenesis, causes " three cause " of cell mutation effect; Extensively be present in the agricultural product such as peanut, corn, wheat class, paddy, serious harm people, animal, avian health.Aflatoxin has B1, B2, G1, G2, M1, M2 etc. multiple, wherein aflatoxin B1 is acknowledged as the strongest natural materials of current carcinogenicity, and within 1993, aflatoxin delimited as I class carcinogenic substance by the Agency for Research on Cancer of the World Health Organization (WHO) (WHO).Research finds, aspergillus flavus is modal fungi in grain and food, but and not all aspergillus flavus strain can produce toxin, according to people's reports such as Matles, from the aspergillus flavus strain that occurring in nature is separated, only having 10% can toxin producing.Therefore, the detection carrying out toxigenic bacterium strain is necessary.
Can fluorescence be there is in aflatoxin under UV-irradiation.AFB1 and AFB2 can produce blue-fluorescence, AFG1 and AFG2 produces green fluorescence.But current market there is no the special equipment for detecting aflatoxin.
Summary of the invention
The purpose of this utility model is, provides a kind of aflatoxin ultraviolet fluorescent detecting device that accurately can detect aflatoxin.
The utility model solves the technical scheme that its technical matters adopts: a kind of aflatoxin ultraviolet fluorescent detecting device, comprise culture tank, Ultraviolet radiation light source, detecting device, described detecting device comprises grating, condenser, fluorescence spectrophotometer mirror, fluorescence display plate, grating is arranged on directly over culture tank, condenser is fixed on directly over grating, fluorescence spectrophotometer mirror is arranged on directly over condenser, fluorescence display plate is arranged on the top of fluorescence spectrophotometer mirror, described fluorescence display plate is fluorescence projection plate, Ultraviolet radiation light source is a luminescence-utraviolet device, the head of illuminator is corresponding with culture tank to be arranged.
Preferably, also comprise detection pedestal, detect pedestal and comprise base, mounting table, culture tank is placed on mounting table, and mounting table is connected by adjusting screw(rod) with base, forms lifting structure.
Preferably, also comprise adjustment seat, adjustment seat is provided with a dwang, and Ultraviolet radiation light source is arranged on dwang by rotating shaft is rotating.
Said structure when in use, nutrient culture media is placed on mounting table, then regulate Ultraviolet radiation light source to irradiate nutrient culture media, make it that fluorescence occur, by carrying out optically focused by condenser after the screening of grating, adding the intensity of high light, then carrying out light splitting by spectroscope and projecting on fluorescence display plate, just significantly can observe the color of fluorescence, the situation of aflatoxin can be judged by fluorescence.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation one of the present embodiment.
Embodiment
Known with reference to figure 1, a kind of aflatoxin ultraviolet fluorescent of the utility model detecting device, comprise culture tank 1, Ultraviolet radiation light source 2, detecting device, described detecting device comprises grating 3, condenser 4, fluorescence spectrophotometer mirror 5, fluorescence display plate 6, grating 3 is arranged on directly over culture tank 1, condenser ' 4 are fixed on directly over grating 3, fluorescence spectrophotometer mirror 5 is arranged on directly over condenser 4, fluorescence display plate 6 is arranged on the top of fluorescence spectrophotometer mirror 5, described fluorescence display plate 6 is fluorescence projection plate, Ultraviolet radiation light source 2 is a luminescence-utraviolet device, the head of illuminator is corresponding with culture tank 1 to be arranged, also comprise detection pedestal, detect pedestal and comprise base 7, mounting table 8, culture tank 1 is placed on mounting table 8, mounting table 8 is connected by adjusting screw(rod) with base 7, form lifting structure, also comprise adjustment seat 9, adjustment seat 9 is provided with a dwang 91, Ultraviolet radiation light source 2 is arranged on dwang 91 by rotating shaft is rotating.
Because the principle of fluorescence can occur aflatoxin under UV-irradiation.AFB1 and AFB2 can produce blue-fluorescence, AFG1 and AFG2 produces green fluorescence.Be inoculated in by mould in nutrient culture media, cultivate 48-72h for 28-30 DEG C, if produce toxin, nutrient culture media can present special fluorescence under UV-irradiation, and this method limit of identification is 5 μ g/mL nutrient culture media.
The utility model utilizes the These characteristics of aflatoxin, design the device of a band UV-detector (wavelength is set as 355-375nm), whether the aspergillus parasiticus and the aspergillus flavus strain that measure screening acquisition produce aflatoxin, set up aflatoxin Control Technology offer help to the feature and exploitation for studying Aspergillus flavus pollution.
Obviously, above-described embodiment is only used to done citing is clearly described, and the restriction not to embodiment.To those of ordinary skill in the art, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still in protection domain of the present utility model.

Claims (3)

1. an aflatoxin ultraviolet fluorescent detecting device, it is characterized in that: comprise culture tank, Ultraviolet radiation light source, detecting device, described detecting device comprises grating, condenser, fluorescence spectrophotometer mirror, fluorescence display plate, grating is arranged on directly over culture tank, condenser is fixed on directly over grating, fluorescence spectrophotometer mirror is arranged on directly over condenser, fluorescence display plate is arranged on the top of fluorescence spectrophotometer mirror, described fluorescence display plate is fluorescence projection plate, Ultraviolet radiation light source is a luminescence-utraviolet device, and the head of illuminator is corresponding with culture tank to be arranged.
2. according to aflatoxin ultraviolet fluorescent detecting device according to claim 1, it is characterized in that: also comprise detection pedestal, detect pedestal and comprise base, mounting table, culture tank is placed on mounting table, mounting table is connected by adjusting screw(rod) with base, forms lifting structure.
3. according to the aflatoxin ultraviolet fluorescent detecting device described in claim 1 or 2, it is characterized in that: also comprise adjustment seat, adjustment seat is provided with a dwang, and Ultraviolet radiation light source is arranged on dwang by rotating shaft is rotating.
CN201520535444.5U 2015-07-20 2015-07-20 Aflatoxin ultraviolet fluorescent detecting device Expired - Fee Related CN204945039U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520535444.5U CN204945039U (en) 2015-07-20 2015-07-20 Aflatoxin ultraviolet fluorescent detecting device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201520535444.5U CN204945039U (en) 2015-07-20 2015-07-20 Aflatoxin ultraviolet fluorescent detecting device

Publications (1)

Publication Number Publication Date
CN204945039U true CN204945039U (en) 2016-01-06

Family

ID=55012454

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201520535444.5U Expired - Fee Related CN204945039U (en) 2015-07-20 2015-07-20 Aflatoxin ultraviolet fluorescent detecting device

Country Status (1)

Country Link
CN (1) CN204945039U (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106092908A (en) * 2016-05-26 2016-11-09 北京化工大学 A kind of method for quick of fungimycin based on two dynamic white balances of color sensor
CN106442458A (en) * 2016-11-29 2017-02-22 合肥泰禾光电科技股份有限公司 Device for detecting aflatoxin by aid of ultraviolet fluorescence spectra in online manner
CN107144554A (en) * 2017-06-16 2017-09-08 合肥泰禾光电科技股份有限公司 A kind of aflatoxin detection means
CN109142256A (en) * 2017-06-14 2019-01-04 深圳市春宏实业有限公司 A kind of portable aflatoxins detection device and method
CN109781676A (en) * 2017-11-10 2019-05-21 陕西秦云农产品检验检测有限公司 A kind of aflatoxin biodegrade testing tool and the method for breeding using it

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106092908A (en) * 2016-05-26 2016-11-09 北京化工大学 A kind of method for quick of fungimycin based on two dynamic white balances of color sensor
CN106442458A (en) * 2016-11-29 2017-02-22 合肥泰禾光电科技股份有限公司 Device for detecting aflatoxin by aid of ultraviolet fluorescence spectra in online manner
CN109142256A (en) * 2017-06-14 2019-01-04 深圳市春宏实业有限公司 A kind of portable aflatoxins detection device and method
CN107144554A (en) * 2017-06-16 2017-09-08 合肥泰禾光电科技股份有限公司 A kind of aflatoxin detection means
CN109781676A (en) * 2017-11-10 2019-05-21 陕西秦云农产品检验检测有限公司 A kind of aflatoxin biodegrade testing tool and the method for breeding using it

Similar Documents

Publication Publication Date Title
CN204945039U (en) Aflatoxin ultraviolet fluorescent detecting device
Hage‐Ahmed et al. Alterations in Root exudation of intercropped tomato mediated by the arbuscular mycorrhizal fungus G lomus mosseae and the soilborne pathogen F usarium oxysporum f. sp. lycopersici
Kharwar et al. Diversity and antimicrobial activity of endophytic fungal community isolated from medicinal plant Cinnamomum camphora
Gaafar et al. In Vitro antioxidant and antimicrobial activities of Lignan flax seed extract (Linum usitatissimum, L.)
Li et al. Effects of relative humidity on infection, colonization and conidiation of M agnaporthe orzyae on perennial ryegrass
Muhsin et al. Bioactive compounds from a polypore fungus Ganoderma applanatum (Per s. ex Wallr.) Pat
Pardo et al. Effects of water activity and temperature on germination and growth profiles of ochratoxigenic Penicillium verrucosum isolates on barley meal extract agar
Menta et al. Does the natural “microcosm” created by Tuber aestivum affect soil microarthropods? A new hypothesis based on Collembola in truffle culture
Babu et al. Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants
Abkhoo et al. Evidence of Alternaria alternata causing leaf spot of Aloe vera in Iran
Soliman et al. Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds
Trinchera et al. Mycorrhiza‐mediated interference between cover crop and weed in organic winter cereal agroecosystems: The mycorrhizal colonization intensity indicator
Gangwar et al. Comparative antagonistic potential of fungal and bacterial bioagents against isolates of Xanthomonas oryzae pv. oryzae
Al-Azzawi et al. Preliminary phytochemical and antibacterial screening of Sesuvium portulacastrum in the United Arab Emirates
Zhang et al. Antimicrobial metabolites from the endophytic fungus Aspergillus sp. of Eucommia ulmoides
Shafique et al. Biological control potential of parthenium hysterophorus against fusarium solani-a cause of fusarium wilt in potato
D'Angiolillo et al. In vitro cultures of Bituminaria bituminosa: pterocarpan, furanocoumarin and isoflavone production and cytotoxic activity evaluation
Hernández-Tasco et al. Endophytic fungus Cladosporium sp (AC-1) isolated from leaves of Annona cacans (Annonaceae) shows high metabolic plasticity to produce bioactive molecules
Rossiana et al. Antibacterial activities of endophytic fungi from mangrove plants Rhizophora apiculata L. and Bruguiera gymnorrhiza (L.) Lamk. on Salmonella typhi
CN101418332A (en) Method for detecting rice blast pathogenic protein
Knaus et al. Monitoring the metabolic state of fungal hyphae and the presence of melanin by Nonlinear Spectral Imaging
Labuda et al. Mycological survey of ripped service tree fruits (Sorbus domestica L.) with an emphasis on toxinogenic fungi
DE602006016724D1 (en) METHOD FOR THE PRODUCTION OF GLUTEN-FREE CULTURES OF THE GENUS PENICILLIUM AND THEIR USE IN BLOUSE MILK PRODUCTS
Teimoori et al. Chemically and physically induced mutagenesis in basidiospores of oyster mushroom Pleurotus ostreatus var. florida
Khairani et al. Seed-borne fungi associated with foxtail millet (Setaria italica L. Beauv.) genotype ICERI-6

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160106

Termination date: 20170720