CN106092908A - A kind of method for quick of fungimycin based on two dynamic white balances of color sensor - Google Patents

Abstract

The method for quick of a kind of fungimycin based on two dynamic white balances of color sensor, belongs to fungimycin detection field.More complicated for the process of current Biological Detection fungimycin, cost is high, be difficult to the problems such as operation, propose a kind of method utilizing double color sensor quickly to detect fungimycin class material concentration, devise a colouring information detecting system, dual sensor is used to realize the dynamic white balance in solution example colouring information gatherer process, so that color detection has adaptivity to the change of ambient light.Use anti-impulse disturbances average value filtering algorithm that the data obtained in gatherer process are processed, the final rgb value obtaining solution example.Finally set up the linear relationship between rgb value and test sample concentration.The present invention makes detecting system have the adaptive ability of higher ambient light.Experiment proves this, and detection method speed is fast, efficiency is high, highly versatile, has higher certainty of measurement and robustness.

Description

A kind of quick detection of fungimycin based on two dynamic white balances of color sensor Method

Technical field

The present invention relates to the method for quick of a kind of fungimycin based on two dynamic white balances of color sensor.

Background technology

At present, food-safety problem is day by day serious, and the safety problem caused by fungimycin class material is the most universal.Very Verticillium toxin (mycotoxins) is that fungus grows produced metabolite in food or feedstuff, its serious threat mankind and dynamic The health and lives safety of thing.Common mycotoxin mainly has aflatoxin (aflatoxins), ochratoxin A (ochratox A), FT (fumonisins) and 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone (zearalenone) etc..Owing to major part is true Mycomycin all has toxicity, and therefore, the content of fungimycin in food-related products has all been formulated strict limit by countries in the world Amount standard.

Aflatoxin in field of food safety, every gram of food is with 10^-9Gram for unit measure, there is the ratio of being detected High, sample distribution is uneven and there is the features such as content is low.Therefore, limit standard and detection technique are proposed higher wanting Ask.At present, the conventional instrument detection method for mycotoxin mainly has thin layer chromatography (TLC), high performance liquid chromatography (HPLC), the instrument analytical method such as mass spectrometer (GCMS) and liquid chromatography mass spectrometric method (LCMS).A kind of high performance liquid chromatography of application Tandem mass spectrometry (HPLC-MS/MS) measures the five kinds of fungimycins analyzed in Semen arachidis hypogaeae and goods thereof, the method is highly sensitive, Selectivity is good.But make a general survey of this kind of method, generally have that sample pretreatment process is more complicated, instrument and equipment is expensive, need to grasp specially Make personnel and the problem such as take time and effort, thus limiting its range of application, and conventional instrument detection method can not meet scene The demand that rapid screening is analyzed.Therefore, the detection pre-treatment of efficient mycotoxin and novel Fast Detection Technique and equipment are developed Particularly important.It is recently proposed a kind of quickly side of detection based on non-marked aptamer Visual retrieval AFB1 Method, utilizes the color change that nanometer gold causes after condensing under Salt treatment, it is achieved that for AFB1 sensitive quickly Visual retrieval.But when based on colorimetric determination, owing to the nanometer gold of different batches is at the absorbance of 520 nanometer wave strong points Slightly difference, when in solution, aptamers and salt ionic concentration are not fixed, absorbance all can be affected, so there being testing result Certain impact.At present, antigen-antibody method, i.e. immunological method are also used for the quick detection to mycotoxin.But it is this Method needs to prepare specific antibody, and antibody itself is owing to having unstability, so the cost that should not preserve and prepare Higher.These deficiencies constrain the immunological method extensive application in mycotoxin field.

In above-described various methods, detection limit is the highest, is not suitable for mycotoxin and there is the feature that content is low. Therefore improve constantly the sensitivity of detection method, strengthen capacity of resisting disturbance, raising detection speed, and reduce testing cost always It it is all the driving source of mycotoxin Fast Detection Technique exploitation.

Summary of the invention

The detection that the present invention is directed to fungimycin content is studied, it is proposed that a kind of bent for field quick detection Huang The technology of mould toxin.Herein for mycotoxin, it is proposed that a kind of detection method utilizing solution colour to determine solution concentration, logical Cross three primary colors (RGB) value using color sensor to obtain solution colour, it is achieved three primary colors mates with variable concentrations solution, from And set up the linear relationship between solution concentration and rgb value.The method has simultaneously improved traditional single-sensor color acquisition system System, uses dual sensor to realize the dynamic white balance of ambient light, makes detecting system have the adaptive ability of higher ambient light. Experiment proves this, and detection method speed is fast, efficiency is high, highly versatile, has higher certainty of measurement and robustness.

A kind of method for quick of fungimycin based on two dynamic white balances of color sensor, it is characterised in that:

The color sensor I three Color Channels by RGB, utilize timer/counter to gather white light in environment Initial data；

Color sensor II, by tri-Color Channels of RGB, utilizes timer/counter to gather the color data of solution；

Utilize Mean Filtering Algorithm, be filtered the initial data of white light in ambient light processing, obtain ambient light White balance parameter；

In data acquisition, there is pulse interference signal in sensor, the solution colour data gathered are carried out anti-arteries and veins Punching interference average value filtering processes；

Utilize white balance parameter that sensor I obtains as the rgb value of benchmark solution colour；

Relation between the two is set up with corresponding rgb value, by the linear relationship having built up, then according to sample concentration Utilize two color sensor systems to remove to detect the colouring information of unknown concentration solution, finally extrapolate corresponding concentration value.

Sensor in Fig. 1 I is detected the corresponding reference frequency of the RGB of white in the ambient light of acquisition and is set to F_r、F_g、 F_b, T_r、T_g、T_bBeing respectively the corresponding reference period of the RGB of white, sensor II detects sample frequency difference during color sample It is set to f_r、f_g、f_b, t_r、t_g、t_bFor detecting sampling period during color sample, according to the Cleaning Principle of color sensor, utilize formula (1), (2) and (3) respectively obtain the rgb value of solution example.

$R=\frac{f_r}{F_r}\×255=\frac{1/t_r}{1/T_r}\×255=\frac{T_r}{t_r}\×255---\left(1\right)$

$G=\frac{f_g}{F_g}\×255=\frac{1/t_g}{1/T_g}\×255=\frac{T_g}{t_g}\×255---\left(2\right)$

$B=\frac{f_b}{F_b}\×255=\frac{1/t_b}{1/T_b}\×255=\frac{T_b}{t_b}\×255---\left(3\right)$

Wherein, owing to color sensor cannot directly obtain measurement frequency during detection, so system is by detection Cycle T and t calculate rgb value.Before calculating RGB, needing to carry out cycle T and t Filtering Processing, wherein T, t are respectively For during sampling pulse signal two cycles that sensor is corresponding.N is sampling number, T_iDuring obtaining ambient light pulse signal A wherein cycle, t_iFor obtaining during sample pulse signal a wherein cycle；

$T=\frac{1}{(N-2)}\×\[\underset{i=1}{\overset{N}{\Σ}}{T}_i-max\left(T_i\right)-min\left(T_i\right)\]---\left(4\right)$

$t=\frac{1}{(N-2)}\×\[\underset{i=1}{\overset{N}{\Σ}}{t}_i-max\left(t_i\right)-min\left(t_i\right)\]---\left(5\right)$

Use two color sensors during detection color, it is possible to the automatization realizing detection data adjusts, tool There is environment self-adaption.Experimental data proof diagram is as follows:

There is advantages that

(1) automatization's feature.Two Color Detecting Systems that the present invention uses can realize automatization's feature of color detection, When ambient light changes, can automatically adjust, it is ensured that the accuracy of testing result, there is environment self-adaption sexual function；

(2) portability feature.This detection device can realize scene, detection on the spot, it is easy to uses, promote；

(3) easy and simple to handle, quick.By the linear relationship set up, can be directly by detection unknown concentration solution Colouring information, i.e. can get the concentration of its correspondence；

(4) highly sensitive.By detection unknown concentration, obtain its relative error and be less than 7%, meet the accurate of detection data Degree requirement；

(5) low cost, profitable.This detection device production cost is low, greatly reduces testing cost.

For the detection of mycotoxin, the quick inspection utilizing two color sensors to realize dynamic white balance in this paper Survey method, does not relies on instrument and equipment and the sample pre-treatments of complexity of costliness, it is not required that prepare specific antibody, only needs By gathering the color change information with analytical solution, set up the linear relationship between solution concentration and three primary colors (RGB).With Time, owing to using two color sensors to measure, it is achieved that white balance real-time from dynamic(al) correction, it is to avoid the change of ambient light is right The impact of testing result, it is ensured that the stability of detecting system and the accuracy of testing result.This detection of experiment show The feasibility of method and effectiveness.The method can be equally used for detecting other about candex kind object matter.

Accompanying drawing explanation

The realization of the dynamic white balance of Fig. 1

The change of rgb value under Fig. 2 varying environment light

Figure (a) and (b) are under two kinds of varying environment light, the situation of change of the R value that single, double sensor records.

Figure (c) and (d) are under two kinds of varying environment light, the situation of change of the G-value that single, double sensor records.

Figure (e) and (f) are under two kinds of varying environment light, the situation of change of the B value that single, double sensor records.

Conclusion:

1, same sample rgb value under varying environment light can fluctuate and changed；

2, when ambient light changes, the data fluctuations that Method for Single Sensor System collects is bigger；

3, when ambient light changes, the data fluctuations that double-sensor system collects is smaller.

Fig. 3 is Color Detecting System circuit diagram, and wherein color sensor model is TCS230, and voltage conversion chip is ULN2003, the power line of color sensor uses 0.01 μ F 0.1 μ F to carry out decoupling, and the Enable Pin OE pin of chip draws with GND Low ESR should be used between foot to connect, to improve noise resisting ability.Fig. 2 is solution example to be detected, is loaded in centrifuge tube, molten Liquid amasss and accounts for the 85% of centrifuge tube volume.

Detailed description of the invention

In the present invention, the preparation of reagent mainly has the preparation of nanometer gold, AFB1 aptamers and nanometer gold under high salt concn Effect.

1, the preparation of nanometer gold

Experiment uses trisodium citrate reduction method to prepare gold colloidal, adds 95.8mL ultra-pure water in three neck round bottom flask, Add 4.2mL mass fraction 1% chlorauric acid solution, magnetic force, be heated at reflux stirring, persistently boil 10min, be subsequently adding 10mL Mass fraction 1% citric acid three sodium solution, solution colour reddens in 1min, continues boiling 20min, the coldest under stirring But to room temperature, the claret liquid obtained, it is nanometer gold.

2, the preparation of solution example

The concentration of AFB1 (AFB1) aptamers and the concentration of NaCl saline solution are this detection method reaction systems Key factor, owing to length, the base composition of different aptamers are the most different, by the aptamers of variable concentrations and NaCl The comparison of solution, determines optimum reaction condition.The optium concentration determining aptamers is 10 μm ol/L, and concentration is 2mol/L's NaCl solution.

Detecting step

Two color sensor systems of the novelty that native system uses during obtaining color sample information, it is achieved Automatization during detection, has adaptivity function, improves the detection stability of data, accuracy.It is simultaneously achieved A kind of method of new detection aflatoxin concentration, specifically comprises the following steps that

The color sensor I three Color Channels by RGB, utilize timer/counter to gather white light in environment Initial data；

Color sensor II, by tri-Color Channels of RGB, utilizes timer/counter to gather the color data of solution；

Utilize Mean Filtering Algorithm, be filtered the initial data of white light in ambient light processing, obtain ambient light White balance parameter；

In data acquisition, there is pulse interference signal in sensor, so carrying out the solution colour data gathered Anti-impulse disturbances average value filtering processes；I.e. remove maximum and the minima value filtering that is averaged again processes；

Utilize white balance parameter that sensor I obtains as the rgb value of benchmark solution colour；

Set up relation between the two according to sample concentration with corresponding rgb value, can be obtained by the linear relationship set up Solution to unknown concentration.

By the linear relationship having built up, two color sensor systems are then utilized to go to detect unknown concentration solution Colouring information, finally extrapolates corresponding concentration value.

Claims (1)

1. the method for quick of fungimycins based on two dynamic white balances of color sensor, it is characterised in that:

Color sensor I three Color Channels by RGB, utilize intervalometer sum counter to gather the original of white light in environment Data；

Color sensor II, by tri-Color Channels of RGB, utilizes intervalometer sum counter to gather the color data of solution；

Utilize Mean Filtering Algorithm, be filtered the initial data of white light in ambient light processing, obtain the white flat of ambient light Weighing apparatus parameter；

In data acquisition, there is pulse interference signal in sensor, the solution colour data gathered is carried out anti-pulse and does Disturb average value filtering to process；

Utilize white balance parameter that sensor I obtains as the rgb value of benchmark solution colour；

Set up relation between the two according to sample concentration with corresponding rgb value, by the linear relationship having built up, then utilize Two color sensor systems remove to detect the colouring information of unknown concentration solution, finally extrapolate corresponding concentration value.