CN204918453U - Protein isolate purification equipment - Google Patents
Protein isolate purification equipment Download PDFInfo
- Publication number
- CN204918453U CN204918453U CN201520613347.3U CN201520613347U CN204918453U CN 204918453 U CN204918453 U CN 204918453U CN 201520613347 U CN201520613347 U CN 201520613347U CN 204918453 U CN204918453 U CN 204918453U
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- China
- Prior art keywords
- pipe
- ultrafiltration membrane
- ultra
- compounding kettle
- protein separation
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model discloses a protein isolate purification equipment belongs to the bioengineering field. It is including joining in marriage device, ultrafiltration device and switch board, the utility model discloses a protein isolate purification equipment, owing to be provided with allotment device and ultrafiltration device, temperature through earlier stage, pH's etc. Allotment and different through the protein bulk of molecule realizes the separation of protein, realizes purified serialization of protein isolate and automated production, and the separation and purification is efficient, and the energy consumption is low, and automated control has been realized to the PLC controller, the whole transport of pump and the automatic switch of solenoid valve of adopting is easy and simple to handle.
Description
Technical field
The utility model relates to protein separation equipment, belongs to bioengineering field.
Background technology
Protein separation is a challenging job, because protein is present in complicated living things system, and self is unstable, heat or meet some solvent volatility inactivation, therefore isolation technique such as traditional distillation, solvent extraction be not suitable for its separation and purification, and chromatography is used for the separation and purification of protein, though have high-resolution feature, but complex operation, difficulty are amplified, cost is high, and the application in suitability for industrialized production is restricted.
Compared with aforesaid method, ultra-filtration technique is as the typical membrane separation technique of one, then there is high-level efficiency, less energy-consumption, without phase transformation, without the need to adding the plurality of advantages such as any chemical reagent, easy and simple to handle, mild condition, having broad application prospects in the processes such as protein desalination, dealcoholysis, concentrated, fractional separation and endotoxin removal.Ultra-filtration technique be have that efficiency is high, energy consumption is low, without phase transformation, without the need to adding a kind of membrane separation technique of the advantages such as any chemical reagent, easy and simple to handle, mild condition, it is for protein separation tool unique advantage.
Utility model content
The purpose of this utility model is to provide a kind of protein separation equipment, and can realize serialization and the automatic production of protein separation, separation and purification efficiency is high, and energy consumption is low, and automatic controlling level is high, easy and simple to handle.
The utility model is solved the problems of the technologies described above by following technique means:
Protein separation equipment of the present utility model, comprise deployment device, ultra-filtration equipment and housing, described deployment device comprises feed pot and compounding kettle, described feed pot and compounding kettle are provided with whipping appts, described feed pot is connected with compounding kettle by filling tube, described compounding kettle is provided with temperature sensor and pH electrode, described compounding kettle is communicated with ultra-filtration equipment by feed-pipe, described feed-pipe is provided with force (forcing) pump, at least one ultrafiltration membrane pipe is provided with in described ultra-filtration equipment, described ultrafiltration membrane pipe is provided with ceramic membrane, described ultrafiltration membrane pipe is fixed on retaining plate, ultra-filtration equipment is divided into intake zone and discharge zone by described retaining plate, described intake zone is provided with pressure transmitter and scum pipe, described discharge zone is provided with back-purge system and discharge nozzle, described whipping appts, temperature sensor, pH electrode, force (forcing) pump, pressure transmitter is all connected with housing, PLC and push button is provided with in described housing.
Further, described whipping appts comprises variable-frequency motor.
Further, described filling tube, feed-pipe, scum pipe, back-purge system and discharge nozzle are provided with magnetic valve.
Further, described compounding kettle is outside equipped with chuck.
Further, the junction of described ultrafiltration membrane pipe and retaining plate is provided with sealing-ring, and described ultrafiltration membrane pipe is hollow structure.
Further, described back-purge system comprises backwashing pump.
The beneficial effects of the utility model: protein separation equipment of the present utility model, owing to being provided with deployment device and ultra-filtration equipment, by the allotment of temperature, pH etc. and the varying in size by protein molecule in early stage, realize the separation of protein, realize serialization and the automatic production of protein separation, separation and purification efficiency is high, energy consumption is low, PLC achieves Automated condtrol, the omnidistance employing conveying of pump and the auto-switch of magnetic valve, easy and simple to handle.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is further described.
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the vertical view of the utility model retaining plate.
Wherein: 1, deployment device, 2, ultra-filtration equipment, 3, housing, 4, feed pot, 5, compounding kettle, 6, whipping appts, 7, filling tube, 8, temperature sensor, 9, pH electrode, 10, feed-pipe, 11, force (forcing) pump, 12, ultrafiltration membrane pipe, 13, ceramic membrane, 14, retaining plate, 15, pressure transmitter, 16, scum pipe, 17, back-purge system, 18, discharge nozzle, 19, PLC, 20, push button, 21, variable-frequency motor, 22, sealing-ring, 23, backwashing pump.
Embodiment
Below with reference to accompanying drawing, the utility model is described in detail, as depicted in figs. 1 and 2: a kind of protein separation equipment of the present embodiment, comprise deployment device 1, ultra-filtration equipment 2 and housing 3, described deployment device 1 comprises feed pot 4 and compounding kettle 5, described feed pot 4 and compounding kettle 5 are provided with whipping appts 6, described feed pot 4 is connected with compounding kettle 5 by filling tube 7, described compounding kettle 5 is provided with temperature sensor 8 and pH electrode 9, described compounding kettle 5 is communicated with ultra-filtration equipment 2 by feed-pipe 10, described feed-pipe 10 is provided with force (forcing) pump 11, at least one ultrafiltration membrane pipe 12 is provided with in described ultra-filtration equipment 2, described ultrafiltration membrane pipe 12 is provided with ceramic membrane 13, described ultrafiltration membrane pipe 12 is fixed on retaining plate 14, ultra-filtration equipment 2 is divided into intake zone and discharge zone by described retaining plate 14, described intake zone is provided with pressure transmitter 15 and scum pipe 16, described discharge zone is provided with back-purge system 17 and discharge nozzle 18, described whipping appts 6, temperature sensor 8, pH electrode 9, force (forcing) pump 11, pressure transmitter 15 is all connected with housing 3, PLC 19 and push button 20 is provided with in described housing 3.
As the further improvement of technique scheme, described whipping appts 6 comprises variable-frequency motor 21.The control of stirring velocity can be carried out.
As the further improvement of technique scheme, described filling tube 7, feed-pipe 10, scum pipe 16, back-purge system 17 and discharge nozzle 18 are provided with magnetic valve.Can be used for the auto-switch of valve, realize automatic production, enhance productivity.
As the further improvement of technique scheme, described compounding kettle 5 is outside equipped with chuck.Can be used for the temperature controlling compounding kettle.
As the further improvement of technique scheme, described ultrafiltration membrane pipe 12 is provided with sealing-ring 22 with the junction of retaining plate 14, and described ultrafiltration membrane pipe 12 is hollow structure.Effectively can carry out ultra-filtration and separation, ultrafiltration membrane pipe is hollow structure, the solution penetrating ultrafiltration membrane pipe can be entered discharge zone through hollow structure.
As the further improvement of technique scheme, described back-purge system 17 comprises backwashing pump 23.Can be used for back flushing ultrafiltration membrane pipe, prevent ultrafiltration membrane pipe from blocking, enhance productivity, improve the work-ing life of ultrafiltration membrane pipe.
Through protein soln to be separated through allotment in early stage, after allotment as pH, temperature, electrolyte concentration etc., enter in ultra-filtration equipment by force (forcing) pump, due to varying in size of protein molecule or varying in size of protein molecule and impurity molecule, the separation and purification of protein can be realized, be provided with back-purge system simultaneously, can be used for back flushing ultrafiltration membrane pipe, PLC and push button is provided with in housing, can be used for automatically controlling various magnetic valve, pump, motor, realize Programmed control.
What finally illustrate is, above embodiment is only in order to illustrate the technical solution of the utility model and unrestricted, although be described in detail the utility model with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify to the technical solution of the utility model or equivalent replacement, and not departing from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of right of the present utility model.
Claims (6)
1. a protein separation equipment, it is characterized in that: comprise deployment device (1), ultra-filtration equipment (2) and housing (3), described deployment device (1) comprises feed pot (4) and compounding kettle (5), described feed pot (4) and compounding kettle (5) are provided with whipping appts (6), described feed pot (4) is connected with compounding kettle (5) by filling tube (7), described compounding kettle (5) is provided with temperature sensor (8) and pH electrode (9), described compounding kettle (5) is communicated with ultra-filtration equipment (2) by feed-pipe (10), described feed-pipe (10) is provided with force (forcing) pump (11), at least one ultrafiltration membrane pipe (12) is provided with in described ultra-filtration equipment (2), described ultrafiltration membrane pipe (12) is provided with ceramic membrane (13), described ultrafiltration membrane pipe (12) is fixed on retaining plate (14), ultra-filtration equipment (2) is divided into intake zone and discharge zone by described retaining plate (14), described intake zone is provided with pressure transmitter (15) and scum pipe (16), described discharge zone is provided with back-purge system (17) and discharge nozzle (18), described whipping appts (6), temperature sensor (8), pH electrode (9), force (forcing) pump (11), pressure transmitter (15) is all connected with housing (3), PLC (19) and push button (20) is provided with in described housing (3).
2. protein separation equipment according to claim 1, is characterized in that: described whipping appts (6) comprises variable-frequency motor (21).
3. protein separation equipment according to claim 1, is characterized in that: described filling tube (7), feed-pipe (10), scum pipe (16), back-purge system (17) and discharge nozzle (18) are provided with magnetic valve.
4. protein separation equipment according to claim 1, is characterized in that: described compounding kettle (5) is outside equipped with chuck.
5. protein separation equipment according to claim 4, is characterized in that: described ultrafiltration membrane pipe (12) is provided with sealing-ring (22) with the junction of retaining plate (14), and described ultrafiltration membrane pipe (12) is hollow structure.
6. protein separation equipment according to claim 4, is characterized in that: described back-purge system (17) comprises backwashing pump (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520613347.3U CN204918453U (en) | 2015-08-15 | 2015-08-15 | Protein isolate purification equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520613347.3U CN204918453U (en) | 2015-08-15 | 2015-08-15 | Protein isolate purification equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204918453U true CN204918453U (en) | 2015-12-30 |
Family
ID=54967600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520613347.3U Expired - Fee Related CN204918453U (en) | 2015-08-15 | 2015-08-15 | Protein isolate purification equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204918453U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109569300A (en) * | 2018-12-14 | 2019-04-05 | 上海景峰制药有限公司 | A kind of system and method for separation of fermentative broth purifying |
-
2015
- 2015-08-15 CN CN201520613347.3U patent/CN204918453U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109569300A (en) * | 2018-12-14 | 2019-04-05 | 上海景峰制药有限公司 | A kind of system and method for separation of fermentative broth purifying |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151230 Termination date: 20160815 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |