CN204832103U - Microorganism galvanotaxis survey device - Google Patents
Microorganism galvanotaxis survey device Download PDFInfo
- Publication number
- CN204832103U CN204832103U CN201520585645.6U CN201520585645U CN204832103U CN 204832103 U CN204832103 U CN 204832103U CN 201520585645 U CN201520585645 U CN 201520585645U CN 204832103 U CN204832103 U CN 204832103U
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- Prior art keywords
- double dish
- microorganism
- anode
- culture dish
- negative electrode
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- 244000005700 microbiome Species 0.000 title claims abstract description 46
- 230000005109 electrotaxis Effects 0.000 title claims abstract description 28
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 12
- 229910052719 titanium Inorganic materials 0.000 claims description 12
- 239000010936 titanium Substances 0.000 claims description 12
- 238000005070 sampling Methods 0.000 claims description 11
- 238000005424 photoluminescence Methods 0.000 abstract description 2
- 230000010415 tropism Effects 0.000 abstract description 2
- 230000005518 electrochemistry Effects 0.000 abstract 2
- 230000035605 chemotaxis Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 240000001131 Nostoc commune Species 0.000 description 1
- 235000013817 Nostoc commune Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000025969 response to chemical stimulus Effects 0.000 description 1
- 230000029305 taxis Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a microorganism galvanotaxis survey device. The device is by electrochemistry workstation, special microorganism galvanotaxis culture dish and signal of telecommunication data acquisition instrument triplex. Special microorganism galvanotaxis culture dish includes culture dish base and culture dish face lid, and the culture dish base sets up positive pole and negative pole alone by the three compartments of share such as division board, every compartment, and positive pole and negative pole link to each other with electrochemistry workstation, signal of telecommunication data acquisition instrument's corresponding interface. Culture dish face lid can bayonet lid in base top, and every part can the exclusive use. The utility model discloses can survey tropism and the physio -biochemical characteristics of microorganism under the electro photoluminescence, it is experimental to carry out three groups simultaneously, improves research efficiency, set up the special microorganism galvanotaxis culture dish face lid of combination formula, the different parts that can take the face lid alone out, the loaded down with trivial details operation that can avoid ordinary culture dish need lift the lid also can reduce the biological pollution that the operation brought.
Description
Technical field
The utility model belongs to field of environment microorganism, is specifically related to a kind of microorganism electrotaxis determinator.
Background technology
Microbes shows taxis in the environment, especially common with chemotaxis.Chemotaxis is the important physiological and biochemical property of microorganism, the chemotaxis of microorganisms, has vital role for disclosing the behavior of microorganism, distribution and performance.In the last few years, microbiological fuel cell (MFC) became the study hotspot of microorganism, the energy and environmental area.In MFC research, it is found that microorganism not only can produce response to chemical substance, also can produce response to electric signal, merit attention.
To bioelectrochemical research, mainly concentrate on MFC and microorganism electrolysis cell (MEC) at present, and be mainly in the macroscopic aspect of test MFC and MEC serviceability, to the microbial action mechanism of its microcosmic point, hitherto reported is few, then rarely has relate to the research of the behavior of microorganism electrotaxis and physio-biochemical characteristics thereof in MFC and MEC.Investigate the response of microorganism to electric signal, need to adopt Bioelectrochemical device to measure.But, still there is no ready-made microorganism electrotaxis determinator in the market.
Summary of the invention
The purpose of this utility model solves problems of the prior art, and provide a kind of microorganism electrotaxis determinator.
A kind of microorganism electrotaxis determinator, device is made up of electrochemical workstation, special microorganism electrotaxis double dish and electric signal data sampling instrument;
Special microorganism electrotaxis double dish comprises double dish base and double dish cover, and double dish base is cylindrical, and is divided into the compartment of N number of segment shape by demarcation strip, N be more than or equal to 3 integer; Each compartment inner bottom part arranges the anode be made up of arc line shaped titanium silk and the negative electrode be made up of fold-line-shaped titanium silk, and the radian that the radian of anode is fan-shaped with this compartment bottom surface is consistent, and two radius angles that the angle of negative electrode is fan-shaped with this compartment bottom surface are consistent; Double dish cover is fan-shaped, and quantity is N number of, and double dish cover two bottom sides radial direction is equipped with L-type slide rail, and demarcation strip upper portion side wall is provided with the groove matched with L-type slide rail, and each double dish cover can independently carry out inserting or extracting; Anode is vertically holded up one section of link titanium silk through double dish cover, as positive contact; Negative electrode is also vertically holded up one section of link titanium silk through double dish cover, as negative contact; The anode of electrochemical workstation is connected with negative contact with positive contact respectively with negative electrode, and the high voltage end of electric signal data sampling instrument is also connected with negative contact with positive contact respectively with low-voltage end.
As preferably, described N=3.
Use a microorganism electrotaxis assay method for described device, comprise the steps:
First, double dish cover and double dish base are closed, and whole special microorganism electrotaxis double dish is carried out sterilizing and is cooled to room temperature;
Secondly, operate in the superclean bench through ultraviolet disinfection, by extracting out respectively along demarcation strip of double dish cover, in the compartment of segment shape, add target nutrient culture media, vaccination target microorganism, after completing, double dish cover is inserted original position;
Finally, the electrode potential of double dish cover is controlled with electrochemical workstation, with voltage and the electric current of electric signal data sampling instrument record microorganism electrotaxis double dish.
The beneficial effects of the utility model are:
(1) anode and negative electrode are set in microbial culture dish, tropism and the physio-biochemical characteristics of microorganism under electro photoluminescence can be observed.
(2) special microorganism double dish is divided into three compartments, three groups of tests can be carried out simultaneously, improve Efficiency.
(3) combined type microbial culture dish cover is set, the different piece of cover can be extracted separately out, Nostoc commune Vanch ware can be avoided to need the troublesome operation of flip, also can reduce the biological pollution operating and bring.
Accompanying drawing explanation
Fig. 1 is microorganism electrotaxis determinator schematic diagram;
Fig. 2 is special microorganism double dish schematic diagram of the present utility model;
Fig. 3 is double dish cover schematic diagram of the present utility model.
In figure: electrochemical workstation 1, special microorganism double dish 2, electric signal data sampling instrument 3, double dish base 4, double dish base demarcation strip 5, anode 6, negative electrode 7, double dish cover 8, L-type slide rail 9.
Embodiment
Below in conjunction with accompanying drawing, the utility model is further illustrated.
As shown in Figure 1, a kind of microorganism electrotaxis determinator, device is made up of electrochemical workstation 1, special microorganism electrotaxis double dish 2 and electric signal data sampling instrument 3; Electrochemical workstation 1 is for controlling the electrode potential of special microorganism double dish 2 Anodic 6 and negative electrode 7.Electric signal data sampling instrument 3 is for monitoring voltage and the electric current of special microorganism double dish 2.
As shown in Figure 2, special microorganism electrotaxis double dish 2 comprises double dish base 4 and double dish cover 8, and double dish base 4 is cylindrical, and the compartment of N number of segment shape is divided into by demarcation strip 5, N be more than or equal to 3 integer, as 4,5 etc., can set as required; The compartment inner bottom part of each segment shape arranges the anode 6 be made up of arc line shaped titanium silk and the negative electrode 7 be made up of fold-line-shaped titanium silk.Anode 6 circular arc fan-shaped along bottom surface is arranged, and the radian that the radian of anode 6 is fan-shaped with this compartment bottom surface is consistent.Negative electrode 7 two radiuses fan-shaped along bottom surface are arranged, and two radius angles that the angle of negative electrode 7 is fan-shaped with this compartment bottom surface are consistent.Anode 6 is not connected with negative electrode 7, but both are relative, forms a space do not closed.The compartment top of each segment shape is provided with in fan-shaped double dish cover 8, and quantity is identical with compartment quantity, is also N number of.
As shown in Figure 3, the demarcation strip 5 that double dish cover 8 inserts the compartment both sides of segment shape is fixed, concrete mode is: double dish cover 8 two bottom sides radial direction is equipped with L-type slide rail 9, demarcation strip 5 upper portion side wall is provided with the groove matched with L-type slide rail 9, by L-type slide rail 9 recessed coordinating with groove, make each double dish cover 8 independently carry out inserting or extracting, thus reduce the contaminated possibility of double dish.When double dish cover 8 inserts demarcation strip 5, the upper surface of double dish cover 8 is higher than base 4 top.Environmental contaminants can be made like this can not to fall in double dish.
Anode 6 is vertically holded up and is connected with one section of link titanium silk through double dish cover 8, as anode 6 joint, negative electrode 7 is also vertically holded up the link titanium silk of connection one section through double dish cover 8, as negative electrode 7 joint, be convenient to be connected with electric signal data sampling instrument with electrochemical workstation.The anode of electrochemical workstation 1 is connected with negative electrode 7 joint with anode 6 joint respectively with negative electrode, the high voltage end of electric signal data sampling instrument 3 is also connected with negative electrode 7 joint with anode 6 joint respectively with low-voltage end and (only illustrates with pair of electrodes in Fig. 1, if there is multipair electrode, each electrode all needs to be connected with corresponding interface).
As a kind of preferred implementation, described N=3.Namely double dish base 4 is divided into the compartment of 3 segment shapes by demarcation strip 5, and the fan-shaped radian in bottom surface of each compartment is 120 °.Such demand that can meet ordinary test, can carry out three groups of tests simultaneously, improves Efficiency.
Use a microorganism electrotaxis assay method for described device, comprise the steps:
First, double dish cover 8 and double dish base 4 are closed, and whole special microorganism electrotaxis double dish 2 is carried out sterilizing 30min under the high temperature of 121 DEG C and is cooled to room temperature;
Secondly, operate in the superclean bench through ultraviolet disinfection, the groove along demarcation strip 5 of the double dish cover 8 on above-mentioned special microorganism electrotaxis double dish 2 is extracted out respectively, and the target nutrient culture media added in the compartment of segment shape needed for test, and vaccination target microorganism, after completing, double dish cover 8 is inserted original position and double dish is carried out airtight;
Finally, control the electrode potential of double dish cover 8 with electrochemical workstation 1, record voltage and the electric current of microorganism electrotaxis double dish 2 with electric signal data sampling instrument 3, thus realize the galvanotactic mensuration of microorganism.
Claims (2)
1. a microorganism electrotaxis determinator, is characterized in that, device is made up of electrochemical workstation (1), special microorganism electrotaxis double dish (2) and electric signal data sampling instrument (3);
Special microorganism electrotaxis double dish (2) comprises double dish base (4) and double dish cover (8), double dish base (4) is for cylindrical, and be divided into the compartment of N number of segment shape by demarcation strip (5), N be more than or equal to 3 integer; Each compartment inner bottom part arranges the anode (6) be made up of arc line shaped titanium silk and the negative electrode (7) be made up of fold-line-shaped titanium silk, the radian that the radian of anode (6) is fan-shaped with this compartment bottom surface is consistent, and two radius angles that the angle of negative electrode (7) is fan-shaped with this compartment bottom surface are consistent; Double dish cover (8) is in fan-shaped, quantity is N number of, double dish cover (8) two bottom sides radial direction is equipped with L-type slide rail (9), demarcation strip (5) upper portion side wall is provided with the groove matched with L-type slide rail (9), and each double dish cover (8) can independently carry out inserting or extracting; Anode (6) is vertically holded up one section of link titanium silk through double dish cover (8), as anode (6) joint; Negative electrode (7) is also vertically holded up one section of link titanium silk through double dish cover (8), as negative electrode (7) joint; The anode of electrochemical workstation (1) is connected with negative electrode (7) joint with anode (6) joint respectively with negative electrode, and the high voltage end of electric signal data sampling instrument (3) is also connected with negative electrode (7) joint with anode (6) joint respectively with low-voltage end.
2. microorganism electrotaxis determinator as claimed in claim 1, is characterized in that, described N=3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520585645.6U CN204832103U (en) | 2015-08-06 | 2015-08-06 | Microorganism galvanotaxis survey device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520585645.6U CN204832103U (en) | 2015-08-06 | 2015-08-06 | Microorganism galvanotaxis survey device |
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| CN204832103U true CN204832103U (en) | 2015-12-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520585645.6U Withdrawn - After Issue CN204832103U (en) | 2015-08-06 | 2015-08-06 | Microorganism galvanotaxis survey device |
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| CN (1) | CN204832103U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044176A (en) * | 2015-08-06 | 2015-11-11 | 浙江大学 | Microorganism galvanotaxis determination device and method |
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2015
- 2015-08-06 CN CN201520585645.6U patent/CN204832103U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044176A (en) * | 2015-08-06 | 2015-11-11 | 浙江大学 | Microorganism galvanotaxis determination device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20151202 Effective date of abandoning: 20180529 |