CN204803325U - Permeability cell culture device is selected to two rooms - Google Patents
Permeability cell culture device is selected to two rooms Download PDFInfo
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- CN204803325U CN204803325U CN201520354728.4U CN201520354728U CN204803325U CN 204803325 U CN204803325 U CN 204803325U CN 201520354728 U CN201520354728 U CN 201520354728U CN 204803325 U CN204803325 U CN 204803325U
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- 238000004113 cell culture Methods 0.000 title claims abstract description 183
- 230000035699 permeability Effects 0.000 title abstract 2
- 238000011081 inoculation Methods 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims description 58
- 239000007788 liquid Substances 0.000 claims description 12
- 230000001413 cellular effect Effects 0.000 claims description 7
- 210000005239 tubule Anatomy 0.000 claims description 6
- 239000000463 material Substances 0.000 abstract description 10
- 238000012549 training Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 50
- 102000004127 Cytokines Human genes 0.000 description 15
- 108090000695 Cytokines Proteins 0.000 description 15
- 150000003384 small molecules Chemical class 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 229920001184 polypeptide Polymers 0.000 description 10
- 102000004196 processed proteins & peptides Human genes 0.000 description 10
- 108090000765 processed proteins & peptides Proteins 0.000 description 10
- 210000004748 cultured cell Anatomy 0.000 description 9
- 239000000203 mixture Substances 0.000 description 7
- 229920003023 plastic Polymers 0.000 description 7
- 239000004033 plastic Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 5
- 230000004913 activation Effects 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 231100000033 toxigenic Toxicity 0.000 description 3
- 230000001551 toxigenic effect Effects 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000003995 blood forming stem cell Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000004405 cytokine-induced killer cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000007124 immune defense Effects 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Abstract
The utility model discloses a permeability cell culture device is selected to two rooms, wherein, the device includes: 2 at least cell culture rooms: a cell culture room and the 2nd cell culture room, wherein, a cell culture room is used for training the cell of supporting that can secrete special material, the 2nd cell culture room is used for accepting special material with the training objective cell, a cell culture room is connected with the 2nd cell culture room through a pellicle, a cell culture room and the 2nd cell culture room all are provided with a feed liquor and inoculation passageway. Through the utility model discloses can effectively reduce the cultivation cost of target cell, and device simple structure easily realizes having good market perspective.
Description
Technical field
The present invention relates to technical field of cell culture, particularly relate to a kind of two rooms selective penetrated property cell culture apparatus.
Background technology
Cell cultures is the gordian technique of widespread use in life and regenerative medicine field, adopt common minimum medium (DMEM or RIPM1640) and additionally add the cultivation that foetal calf serum can realize conventional mammalian cell, but for needing extra some specific type cells adding many cytokines etc., often cell cultures cost is very high for the cell culture system of this routine.Therefore, to the cell of this specific type, reduce the key issue that cell cultures cost becomes Sustainable development.
Along with the rise of cell therapy personalization technology, the cell of amplification in vitro special efficacy has become the emphasis development project of preclinical study and clinical treatment.The cell with amplification in vitro special efficacy comprises by multi-functional hemopoietic stem cell, T cell, NK cell, DC cell and CIK cell etc., feed back to after this kind of cell expansion ex vivo again in lactation organism, be conducive to the hematopoietic device function improving body: as promoted quantity and function maintenance, anti-tumor capacity and the immune defense ability of red corpuscle, immunocyte etc.
But the cell of amplification in vitro special efficacy must add cytokine profiles, albumen or antibody, under conventional medium, cultivation can cause cost high and the problem of complicated operation, and existing market does not have a kind of technical scheme that can effectively solve the problem.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of two rooms selective penetrated property cell culture apparatus, be intended to solve conventional medium in prior art and cultivate and need extra the cost high and problem of complicated operation when adding the cell of particular matter.
Technical scheme of the present invention is as follows:
A kind of two rooms selective penetrated property cell culture apparatus, wherein, described device comprises: at least 2 cell culture chambers: the first cell culture chamber and the second cell culture chamber; Described first cell culture chamber is connected with the second cell culture chamber by a semi-permeable membranes; Described first cell culture chamber and the second cell culture chamber are provided with a feed liquor and inoculation passage.
Described pair of room selective penetrated property cell culture apparatus, wherein, aperture≤0.22 μm of described semi-permeable membranes.
Described pair of room selective penetrated property cell culture apparatus, wherein, described first cell culture chamber and the second cell culture chamber are horizontal positioned, and described first cell culture chamber is connected with the second cell culture chamber by the tubule being provided with a semi-permeable membranes.
Described pair of room selective penetrated property cell culture apparatus, wherein, described first cell culture chamber and the second cell culture chamber are vertical placement, and the bottom of described first cell culture chamber is connected by the top of a semi-permeable membranes with the second cell culture chamber.
Described pair of room selective penetrated property cell culture apparatus, wherein, is also provided with multi-layer cellular and cultivates filter membrane in described first cell culture chamber.
Described pair of room selective penetrated property cell culture apparatus, wherein, described multi-layer cellular cultivates aperture≤0.22 μm of filter membrane.
Described pair of room selective penetrated property cell culture apparatus, wherein, the top of described first cell culture chamber is provided with a non-tight lid, and the bottom of described second cell culture chamber is provided with one for changing the valve system of liquid.
Described pair of room selective penetrated property cell culture apparatus, wherein, described cell culture chamber is fixed on can on shaking apparatus.
Described pair of room selective penetrated property cell culture apparatus, wherein, described can shaking apparatus be shaking table.
Beneficial effect: of the present invention pair of room selective penetrated property cell culture apparatus, it comprises the cell culture chamber that at least 2 are cultivated different cell: the first cell culture chamber and the second cell culture chamber, it passes through a semi-permeable membranes by spaced apart for two cell culture chambers, make the small-molecule substance such as cytokine, antibody that in the first cell culture chamber, cell discharges can enter by its cell cultures is utilized in the second cell culture chamber by semi-permeable membranes, and in two cell culture chambers, institute's cultured cells can not mix through semi-permeable membranes.Effectively can be reduced the toxigenic capacity of target cell by the present invention, and described apparatus structure is simple and easy to realize, and has good market application foreground.
Accompanying drawing explanation
Fig. 1 is the structural representation of the two rooms pocket type cell culture apparatus in the embodiment of the present invention one.
Fig. 2 is the structural representation of the two rooms pot type cell culture apparatus in the embodiment of the present invention two.
Fig. 3 is the structural representation of the many filter membranes type two rooms pot type cell culture apparatus in the embodiment of the present invention three.
Fig. 4 is the structural representation of the interchangeable liquid type two rooms pot type cell culture apparatus in the embodiment of the present invention four.
Fig. 5 is the structural representation of the interchangeable liquid type of the many filter membranes two rooms pot type cell culture apparatus in the embodiment of the present invention four.
Embodiment
The invention provides a kind of two rooms selective penetrated property cell culture apparatus, for making object of the present invention, technical scheme and effect clearly, clearly, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The invention provides a kind of two rooms selective penetrated property cell culture apparatus, wherein, described device comprises: at least 2 cell culture chambers: the first cell culture chamber and the second cell culture chamber; Wherein, described first cell culture chamber is for cultivating the keeping cell secreting particular matter, and described second cell culture chamber is used for accepting particular matter with training objective cell.And described first cell culture chamber is connected with the second cell culture chamber by a semi-permeable membranes; Described first cell culture chamber and the second cell culture chamber are provided with a feed liquor and inoculation passage, and wherein, described feed liquor and inoculation passage are inoculated for adding the materials such as nutritive medium and adding cell.
A semi-permeable membranes is passed through by spaced apart for two cell culture chambers in the embodiment of the present invention, make the small-molecule substance such as cytokine, antibody that in the first cell culture chamber, cell discharges can enter by its cell cultures is utilized in the second cell culture chamber by semi-permeable membranes, and in two cell culture chambers, institute's cultured cells can not mix through semi-permeable membranes.Of the present invention pair of room selective penetrated property cell culture apparatus is specifically applied to the cultivation of cultivating and need add the cell of particular matter, the toxigenic capacity of target cell effectively can be reduced by the present invention, and described apparatus structure is simple and easy to realize, and has good market application foreground.
Particularly, the cell that in each cell culture chamber, single culture is different, the wherein multiple particular matter such as keeping cell energy cytokine, protein polypeptide and antibody of cultivating of the first cell culture chamber, and the target cell that these materials are cultivated in the second cell culture chamber is just required when increasing, by arranging the cell culture chamber that 2 are cultivated different cells, effectively can reduce and needing the extra production cost adding the target cell of particular matter.
In addition, described in the present invention, the first cell culture chamber is connected with the second cell culture chamber by a semi-permeable membranes.Semi-permeable membranes is selectivity through film, it allows ion and small-molecule substance freely to pass through, biomacromolecule such as cell etc. then can not freely pass through, and its reason is because the aperture ratio ion of semi-permeable membranes and greatly micromolecular, but than little as protein and starch of biomacromolecule.Semi-permeable membranes is adopted the first cell culture chamber and the second cell culture chamber to be coupled together in the present invention, the small-molecule substance between two Cytology Labs is made to carry out free exchange, as supported the cytokine secreted by cell in the first cell culture chamber, it is used for the growth of target cell that protein polypeptides etc. enter into the second cell culture chamber by semi-permeable membranes, it has the short activation increasing or promote certain approach to target cell, and the keeping cell cultivated in 2 cell culture chambers and target cell can't be exchanged by semi-permeable membranes, the unicity of effective maintenance cell cultures, cell is avoided to mix.
In preferred embodiment, cell culture chamber of the present invention comprises the culture dish, culture plate, the culture tank that are suitable for attached cell and cultivate, the many filter membranes culture tank being suitable for high-density culture and the culture bag being suitable for suspension cell culture, one or more in culture tank.Dissimilar culture vessel can be adopted as cell culture chamber for described pair of room selective penetrated property cell culture apparatus in the present invention, and described cell culture chamber is provided with a feed liquor and inoculation passage, can be used for adding nutritive medium and carrying out cell inoculation, realize the cultivation to cell.In addition, cell culture chamber of the present invention allows gas as CO
2free in and out.Further, described feed liquor and inoculation passage can be arranged on the top position of described cell culture chamber.
Preferably, aperture≤0.22 μm of described semi-permeable membranes.Particularly, the aperture of described semi-permeable membranes is 0.22 μm, 0.1 μm, certainly, also can need according to actual production the semi-permeable membranes selecting other pore sizes.Semi-permeable membranes of the present invention allows the small-molecule substances such as cytokine, protein polypeptide or antibody freely to pass through, but does not allow passing through of keeping cell or target cell.
In preferred embodiment, described cell culture chamber is fixed on can on shaking apparatus, for promoting that the acellular components between cell culture chamber exchanges.Cell culture chamber described in the present invention can by being fixed on the device that slightly can shake, vibrate or rotate, as being fixed on shaking table, effectively can promote that the acellular components between two cell culture chambers (i.e. cytokine, protein polypeptide or antibody etc.) fully exchanges, meet the needs of target cell growth.
Further, the first cell culture chamber of the present invention and the second cell culture chamber both can be horizontal positioned, also can be vertical placement.When it is horizontal positioned, described first cell culture chamber is connected with the second cell culture chamber by the tubule being provided with a semi-permeable membranes, and now semi-permeable membranes is arranged at the tubule bosom position of connection two cell culture chambers; When it is for vertically placing, the bottom of described first cell culture chamber is connected by the top of a semi-permeable membranes with the second cell culture chamber, now the bottom of the first cell culture chamber and the top of the second cell culture chamber fit together, and the two are separated by means of only a semi-permeable membranes.
More preferably, be also provided with multi-layer cellular in described first cell culture chamber and cultivate filter membrane, it can realize high-density culture attached cell.Further, described multi-layer cellular cultivates aperture≤0.22 μm of filter membrane.
In addition, when the first cell culture chamber is vertical placement with the second cell culture chamber, the top of described first cell culture chamber is provided with a non-tight lid, and the bottom of described second cell culture chamber is provided with one for changing the valve system of liquid.Particularly, described non-tight lid can adopt the various material such as plastics to make, and it covers the top of the first cell culture chamber, can effectively prevent the pollutents such as dust from entering in cell culture chamber, and the lid adopting non-fully to seal, gas in cell culture chamber can be allowed (as CO
2) exchange with the external world.In addition, described valve system can be arranged in the plastic catheter connected bottom the second cell culture chamber, certain described plastic catheter and second culturing room's bottom attachment locations is also provided with a semi-permeable membranes structure, prevents loss cell.When cell is cultivated, valve system is in closing condition, when carrying out changing liquid, valve system is in open mode, now plastic catheter can be connected with vacuum fan by a sebific duct, and remove the old substratum of part by vacuum fan, then add new substratum by changing liquid and inoculating passage.
With specific embodiment, technical solution of the present invention is further elaborated below.
Embodiment one
Two rooms pocket type cell culture apparatus
Two pocket type cell culture apparatus of cultivating that the present embodiment describes possess following features: culture bag material used allows gas (as CO
2) freely pass through, but do not allow other fluid molecule seepages to go out.Be connected by a tubule between two culture bag, have a selectivity semi-permeable membranes 30 in the middle of the tubule 31 be connected, the aperture of selectivity semi-permeable membranes is 0.22 μm.This selectivity semi-permeable membranes 30 allows the cytokine in the first cell culture chamber 10 secreted by cultured cells, protein polypeptide, antibody or other small-molecule substances arrive in the second cell culture chamber 20, for the second cell culture chamber 20 cultured cells utilize, these are by the cytokine of semi-permeable membranes, protein polypeptide, antibody or other small-molecule substances have the activation promoting propagation or have certain approach of promotion to the cell that the second cell culture chamber 20 is inoculated, each culture bag has corresponding cell to inoculate and liquid adding hole 40(is shown in Fig. 1), and described culture bag can be placed on the device that can slightly rotate or vibrate, this rotation or vibration can fully exchange due to the material composition except cell in nutrient solution.
Embodiment two
two rooms pot type cell culture apparatus
Two pot type cell culture apparatus of cultivating that the present embodiment describes possess following features: two cell culture chambers are spatially vertical placements, and the aperture of the selectivity semi-permeable membranes 30 connected in the middle of it is 0.1 μm.This selectivity semi-permeable membranes 30 allows the cytokine in the first cell culture chamber 10 secreted by cultured cells, protein polypeptide, antibody or other small-molecule substances to arrive in the second cell culture chamber 20, for the second cell culture chamber 20 cultured cells utilize, these cytokines by selectivity semi-permeable membranes 30, protein polypeptide, antibody or other small-molecule substances have the activation promoting propagation or have certain approach of promotion to the cell that the second cell culture chamber 20 is inoculated.First cell culture chamber 10 is covered by a lid 50, but is not seal completely, allows gas (as CO
2) occur to exchange (see figure 2) with extraneous.This device can be placed on the device that can slightly rotate or vibrate, and this rotation or vibration can fully exchange due to the material composition except cell in nutrient solution.
Embodiment three
many filter membranes type two rooms pot type cell culture apparatus
The two tank cell culture apparatus room, room of many filter membranes type that the present embodiment describes improves on the basis of embodiment two, concrete improvement is as follows: in the first cell culture chamber 10, be vertically placed with multi-layer cellular cultivate filter membrane 30(and multilayer selectivity semi-permeable membranes), can high-density culture attached cell, the aperture of selectivity semi-permeable membranes is 0.16 μm.Selectivity semi-permeable membranes 30 not only allows cell attachment to grow, cytokine, protein polypeptide, antibody or other small-molecule substances in cell culture chamber secreted by cultured cells is also allowed to arrive the second cell culture chamber 20, for the second cell culture chamber 20 cultured cells utilize, these cytokines by selectivity semi-permeable membranes 30, protein polypeptide, antibody or other small-molecule substances have the activation (see figure 3) promoting propagation or have certain approach of promotion to the cell that the second cell culture chamber 20 is inoculated.This device can be placed on the device that can slightly rotate or vibrate, and this rotation or vibration can fully exchange due to the material composition except cell in nutrient solution.
Embodiment four
the two room pot type cell culture apparatus of interchangeable liquid type and the interchangeable liquid type of many filter membranes two rooms pot type cell culture apparatus
The interchangeable liquid type that the present embodiment describes two rooms pot type cell culture apparatus is in embodiment 2(or embodiment 3) basis on improve.Concrete improvement is as follows: be connected with a plastic catheter 60 in the bottom of the second cell culture chamber 20, and in this plastic catheter 60, be provided with one can the valve 61 of switch, when cell cultures, valve 61 is closed; When liquid is changed in midway, valve 61 is opened.This plastic catheter 60 is connected with vacuum fan by an emulsion tube 70, discharges old substratum when vacuum fan is opened, and adds new substratum (see figure 4) by feed liquor and inoculation passage simultaneously.First cell culture chamber also can be designed to many filter membranes type, to realize the cultivation (see figure 5) of high-density attached cell.This device can be placed on the device that can slightly rotate or vibrate, and this rotation or vibration can fully exchange due to the material composition except cell in nutrient solution.
In sum, of the present invention pair of room selective penetrated property cell culture apparatus, it comprises the cell culture chamber that at least 2 are cultivated different cell: the first cell culture chamber and the second cell culture chamber, it passes through a semi-permeable membranes by spaced apart for two cell culture chambers, make the small-molecule substance such as cytokine, antibody that in the first cell culture chamber, cell discharges can enter by its cell cultures is utilized in the second cell culture chamber by semi-permeable membranes, and in two cell culture chambers, institute's cultured cells can not mix through semi-permeable membranes.Effectively can be reduced the toxigenic capacity of target cell by the present invention, and described apparatus structure is simple and easy to realize, and has good market application foreground.
Should be understood that, application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (9)
1. two rooms selective penetrated property cell culture apparatus, it is characterized in that, described device comprises: at least 2 cell culture chambers: the first cell culture chamber and the second cell culture chamber; Described first cell culture chamber is connected with the second cell culture chamber by a semi-permeable membranes; Described first cell culture chamber and the second cell culture chamber are provided with a feed liquor and inoculation passage.
2. two rooms selective penetrated property cell culture apparatus according to claim 1, is characterized in that, aperture≤0.22 μm of described semi-permeable membranes.
3. two rooms selective penetrated property cell culture apparatus according to claim 1, it is characterized in that, described first cell culture chamber and the second cell culture chamber are horizontal positioned, and described first cell culture chamber is connected with the second cell culture chamber by the tubule being provided with a semi-permeable membranes.
4. two rooms selective penetrated property cell culture apparatus according to claim 1, it is characterized in that, described first cell culture chamber and the second cell culture chamber are vertical placement, and the bottom of described first cell culture chamber is connected by the top of a semi-permeable membranes with the second cell culture chamber.
5. two rooms selective penetrated property cell culture apparatus according to claim 4, is characterized in that, is also provided with multi-layer cellular and cultivates filter membrane in described first cell culture chamber.
6. two rooms selective penetrated property cell culture apparatus according to claim 5, is characterized in that, described multi-layer cellular cultivates aperture≤0.22 μm of filter membrane.
7. two rooms selective penetrated property cell culture apparatus according to claim 4, it is characterized in that, the top of described first cell culture chamber is provided with a non-tight lid, and the bottom of described second cell culture chamber is provided with one for changing the valve system of liquid.
8. two rooms selective penetrated property cell culture apparatus according to claim 1, is characterized in that, described cell culture chamber is fixed on can on shaking apparatus.
9. two rooms selective penetrated property cell culture apparatus according to claim 8, it is characterized in that, described can shaking apparatus be shaking table.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520354728.4U CN204803325U (en) | 2015-05-28 | 2015-05-28 | Permeability cell culture device is selected to two rooms |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520354728.4U CN204803325U (en) | 2015-05-28 | 2015-05-28 | Permeability cell culture device is selected to two rooms |
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Address after: Guangdong city of Shenzhen province Nanshan District Guangdong streets Hyde three No. 126 Houhai Excellence Center 14 1406 Co-patentee after: FULIXIN LIFE TECHNOLOGY CO.,LTD. Patentee after: Chinese Health Industry Group Co.,Ltd. Address before: 518000 Guangdong city of Shenzhen province Nanshan District Guangdong streets Hyde three No. 126 Houhai Excellence Center 14 1406 Co-patentee before: FULIXIN LIFE TECHNOLOGY CO.,LTD. Patentee before: Shenzhen China Health Industry Group Co.,Ltd. |
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