CN204740186U - Heterogeneous liquid subsides automatic monitoring equipment - Google Patents

Abstract

The utility model discloses a heterogeneous liquid subsides automatic monitoring equipment, equipment includes: the sample bears device, image acquisition device, controlling means and optional data processing device. The device has simple structure, easily operation, compare with the artifical measurement mode of tradition have save time, in succession, reliably, advantage such as accuracy, all have great potential using value in a plurality of scientific researches, production application fields such as pharmacy, chemical chemical industry, materialogy, food processings.

Description

Heterogeneous liquid settlement automatic monitoring equipment

Technical field

The utility model relates to field of medicine and chemical technology, relates to a kind of heterogeneous liquid settlement monitoring equipment particularly.

Background technology

Sedimentation refers to that particulate in a fluid under gravity, and the motion of deposition, refers generally to the sedimentation of solia particle in a liquid downwards.The factors such as the motion state of the speed of sedimentation and the density of the particle diameter of particulate, particulate, the density of fluid and viscosity, temperature, fluid are relevant, in different field, for different application, by adding surfactant, suspending agent, stabilizing agent, flocculating agent, polycoagulant, decoagulant, regulate the settling characteristics of heterogeneous system.The mensuration of sedimentation dynamic process, important in inhibiting in the fields such as medicament, chemical industry, food.

In art of pharmacy, the heterogeneous formulation such as glue, emulsion, supensoid agent are widely used clinically as regular dosage form, with incompatible liquid, Solid drug particles for dispersed substance, the heterogeneous system formed for dispersion medium with gaseous state, liquid continuous phase.The sedimentation of heterogeneous formulation system is the principal element affecting its physical stability and clinical efficacy, also be the major criterion weighing preparation product quality, therefore state-promulgated pharmacopoeia regulation supensoid agent needs to check sedimentation volume ratio in quality check, weigh one of whether qualified major criterion of product, sedimentation is too much, too fast, to meaning medicine being made supensoid agent be lost, so sedimentation volume ratio must be up to standard, its product quality of guarantee.Extensively also exist in formulation process simultaneously and the process of heterogeneous system operate, as: dispersion, filtration, centrifugal, clean system etc., study its sedimentation dynamic characteristic, important in inhibiting in heterogeneous formulation Formulation, medical process.

For mixed suspension preparation, according to 2010 editions Chinese Pharmacopoeias, its assay method: test sample 50mL contained by apparatus plug graduated cylinder, close plug, firmly jolting 1 minute, writes down the beginning height H of suspended matter ₀, leave standstill 3 hours, write down the final height H of suspended matter, be calculated as follows:

With sedimentation volumn ratio for ordinate, the settling time is horizontal ordinate mapping, can obtain subsidence curve, can judge the quality of supensoid agent Formulation according to the shape of subsidence curve.The more gentle slow reduction of subsidence curve can think that Formulation is excellent.

In chemical material field, along with widely using of macromolecular material, a large amount of uses particularly in building, traffic and electronic apparatus etc., danger and the harmfulness of fire considerably increase.Adopt the polymeric material adding flame retardant of magnesium hydroxide, traditional halogen containing flame-retardant material can be improved due to thermal decomposition and the burn a large amount of smog of generation and shortcoming that is poisonous, corrosive hydrogen halides gas, for overcoming the shortcoming of magnesium hydroxide dispersiveness, poor compatibility, improve usability, surface modification treatment must be carried out to it and eliminate surface polarity, change the surface nature of magnesium hydroxide, to solve the problem of compatibility and bad dispersibility.With surface modifier, wet process surface treatment is carried out to magnesium hydroxide, test the magnesium hydrate powder before modified by settling velocity and it is characterized, thus determining the process conditions of the surface modifications such as modifier kind, modifier consumption, modification temperature and modification time.

In food processing field, the finished product of food processing field and intermediate major part exist with the form of heterogeneous suspending system, as acidic milk drink can be divided into fermented type and the large class of layout type two by its machining process.The protein beverage of this trophism is difficult to can not keep uniform stability status, easy layering and precipitation, how to ensure that in beverage, the stability of protein is one of numerous food product producer urgent problem.Therefore lactic acid drink to be made within the shelf-life not produce layering or precipitation, improve stability, namely will reduce the settling velocity of particle as much as possible, make it be substantially equal to zero.

Existing sedimentation dynamic process assay method rests on artificial sxemiquantitative observation, trend contrast substantially, lack quantitative dynamic analysis, in practical application, different system sedimentation span cycle length is large, reading sample interval is different, when sample to be tested is too much, the resources costs such as manpower, time is high, and artificial reading error directly affects comparatively greatly the accuracy of result.The mensuration demand of a small amount of/micro-example, multiple batches of parallel control, accurate quantification, long time period in preparation R&D process cannot be met.

Utility model content

The purpose of this utility model is to provide a kind of heterogeneous liquid settlement automatic monitoring method and device, with solve sedimentation kinetic detection methods of the prior art cannot robotization, long period, the problem that accurately detects.

The utility model provides a kind of settlement automatic monitoring equipment of heterogeneous liquid, and described equipment comprises:

Sample bearing device, described sample bearing device is for laying the container of splendid attire testing sample, and wherein said testing sample is the heterogeneous liquid that sedimentation can occur;

Image collecting device, described image collecting device is set to the image information for gathering described sample;

Control device, described control device is set to the work for controlling described image collecting device; With

Optional data processing equipment, described data processing equipment is set to the liquid level separatrix for identifying in described image information, and/or identifies liquid level boundary line further over time.

In another preference, described image collecting device is set to obtain at the image of different time points collected specimens the sample image information comprising many hardwoods image.

In another preference, the image information of described sample is selected from lower group: the intensity of reflected light of sample or fluorescence intensity.

In another preference, described sample bearing device can lay the container of one or more (as 2,3,4,5,6,7,8,9,10) splendid attire testing sample.

In another preference, described data processing equipment comprises data processing module and result derives module, and described data processing module for the treatment of the image information collected, and stores brightness value, positional information and temporal information; Described result derives module for deriving sedimentation kinetics equation and drawing subsidence curve.

In another preference, the settlement automatic monitoring equipment of described heterogeneous liquid also comprises casing and light-emitting device, described sample bearing device, described image collecting device and described light-emitting device are placed in described casing, and described light-emitting device provides light source for described image collecting device; Preferably, the luminous intensity of described light-emitting device is adjustable.

In another preference, described light source be selected from lower group one or more: the micro-light source of LED, LED array light source, LED area light source, shadowless lamp, cold light source bulb (xenon lamp), laser diode and gas laser.

In another preference, described equipment also comprises temperature control equipment, and described temperature control equipment is set to the temperature for regulating sample.

In another preference, described control device is also for controlling described temperature control equipment and/or light-emitting device.

In another preference, described " control " comprises setting and/or changes picture-taken frequency, the image acquisition quantity of described image collecting device; And/or

Set and/or change described thermostatic target temperature; And/or

Set and/or change light intensity and/or the light application time of described light-emitting device.

In another preference, described sample bearing device comprises the support of liquid container and fixing described liquid container.

In another preference, described support is provided with the hole corresponding with described liquid container, described liquid container is laid on the bracket by described hole, described support is provided with longitudinal window, when the described liquid container that testing sample is housed is laid on the bracket, described image collecting device gathers the image information of described sample through described window.In another preference, described container is transparent or semitransparent.Described hole has the space of accommodating described liquid container, when the described liquid container that testing sample is housed is laid on the bracket, the part that testing sample is housed of at least described liquid container is positioned at described hole, and the window that described hole is offered allows the state observing testing sample from outside.

In another preference, the material of described liquid container is selected from: acrylic, quartz glass, simple glass, phenolics and silica gel, preferred quartz glass.

In another preference, described liquid container is flat based tubes.

In another preference, described image collecting device comprises image acquisition device and guide rail, and described image acquisition device is movably arranged on described guide rail.

In another preference, described image acquisition device be selected from lower group one or more: line array CCD, area array CCD, industrial CCD/CMOS, high resolution CCD/CMOS and fluorescent probe.

In another preference, described temperature control equipment comprises temperature sensor, well heater and heating radiator.

In another preference, described temperature control equipment adopts water-bath, metal bath or gas bath to control described sample temperature.In another preference, described support is metal support (being preferably copper, aldary, aluminum or aluminum alloy support), and described temperature control equipment controls the temperature of described sample to be tested by described metal support.

In another preference, described well heater is positioned at the below of described support.

In another preference, described casing is provided with ventilating opening.

In another preference, described casing is camera bellows.

In another preference, described device also comprises background board.

The equipment of monitoring heterogeneous liquid of the present utility model sedimentation, the method for its monitoring heterogeneous liquid sedimentation used comprises step:

A () provides heterogeneous liquid sample and gathers the image information of described sample

Image collecting device is used to obtain at the image of different time points collected specimens the sample image information comprising many hardwoods image;

(b) processing image information

(b1) extract the uniformity coefficient characteristic information (preferably, described uniformity coefficient characteristic information is brightness value) of every hardwood image target area after each hardwood image gray processing step (a) gathered, draw characteristic curve;

(b2) the three-dimensional sedimentation dynamics surface model comprising temporal information is formed after being merged according to image acquisition time by the characteristic curve of each hardwood image.By analyzing this this three-dimensional sedimentation dynamics surface model, namely can obtain the sedimentation dynamic information of heterogeneous liquid sample, comprising sedimentation kinetic curve and sedimentation kinetics equation.

In another preference, described method also comprises step:

(b3) the three-dimensional sedimentation dynamics surface model obtained in step (b2) is processed

Retain region, sedimentation boundary place on three-dimensional sedimentation dynamics surface model, remove other region, obtain sedimentation kinetic curve and the sedimentation kinetics equation of described sample through matching modeling.

In another preference, in described step (b3), the defining method in region, described sedimentation boundary place is: described characteristic curve occurs that the position of sudden change is liquid level boundary region; Preferably, in curve sudden change place, change place within the scope of 20 ° ~ 90 ° of described slope of a curve is liquid level boundary curve.

In another preference, in described step (a), under specified time interval, image acquisition is carried out to described sample and obtain the sample image information comprising many hardwoods sample image.Described specified time interval can be 0.1s-1h, that is, can carry out an image acquisition every 0.1s, and also can carry out an image acquisition every 1h, concrete interval time is depending on sample.

In another preference, in described step (b1), described characteristic curve is the curve that reflection liquid level changes to liquid bottom intensity of brightness.

In another preference, described sedimentation kinetic curve is the time dependent curve of reaction sedimentation volume ratio.

In another preference, described method also comprises step:

C sedimentation kinetic curve that () obtains according to step (b) and equation carry out quantitative evaluation to the stability of heterogeneous liquid sample and export measuring report.

In another preference, in described step (b1), carry out Image semantic classification by after each the hardwood image gray processing obtained in step (a), extract characteristic area, store brightness value and time point information, and draw described characteristic curve.

In another preference, in described step (b1), described Image semantic classification comprises:

(i) image enhaucament: usage space territory method and/or frequency domain method improve visual quality of images;

(ii) filtering: use the method for wave filter and/or Wiener filtering to extract echo signal (brightness value) from noise;

(iii) limb recognition: use Roberts operator, Kirsch operator to detect step change type edge, Laplacian operator is used to detect ridge or edge effect type picture element, use threshold value responsive type Sobel and Prewitt operator to detect fuzzy edge, realize non-threshold values and detect;

(iv) Iamge Segmentation: the image partition method of use comprises the image partition method (threshold method based on region, region-growing method, split degree method), based on image partition method (the Robert operator at edge, Sobel operator, Pre-witt operator, Canny operator, Laplace operator, method of differential operator, frontier tracing method) and mixed type image partition method (genetic algorithm, wavelet transformation, Snake model), image is divided into several are specific, have peculiar property region and propose interesting target (as, settling zone), based on the area in this calculated settlement region.

In another preference, in described step (b), pre-service is carried out to the image collected, reduce the interference of noise, improve the image quality of image; Gray processing is carried out to image, is found out the edge feature of gray level image by target identification, Iamge Segmentation, determine liquid level separatrix, and then draw the level change in every hardwood image; From the image of different time points collection, extract sedimentation boundary feature point set, obtain subsidence curve through matching.

Should be understood that within the scope of the utility model, above-mentioned each technical characteristic of the present utility model and can combining mutually between specifically described each technical characteristic in below (eg embodiment), thus form new or preferred technical scheme.As space is limited, tiredly no longer one by one to state at this.

Accompanying drawing explanation

Figure 1A shows the structural representation of preferably the utility model device;

Figure 1B shows the perspective view of preferably the utility model device;

Fig. 1 C shows the sectional structure schematic diagram of preferably the utility model device;

Fig. 1 D shows the sample bearing device structural representation of preferably the utility model device;

Fig. 1 E shows the preferably sample bearing device of the utility model device and a temperature control equipment structural representation;

Fig. 1 F shows the preferably sample bearing device of the utility model device and a temperature control equipment structural representation;

Fig. 1 G shows the supporting structure schematic diagram of preferably the utility model device;

Fig. 1 H shows the plan structure schematic diagram of preferably the utility model device;

Fig. 2 shows single image and the characteristic curve thereof of embodiment 2;

Fig. 3 shows three typical images and characteristic curve thereof in the settling process of embodiment 2;

Fig. 4 shows the settling characteristics information time sequence three-dimensional surface model of embodiment 2;

Fig. 5 shows the sedimentation boundary detection figure after the settling characteristics information extraction of embodiment 2;

Fig. 6 shows the sedimentation boundary defining method of embodiment 2;

The sedimentation kinetics equation that Fig. 7 shows in sedimentation kinetics equation matched curve Fig. 7 of embodiment 2 is as follows:

Fit equation:

fresult＝0.4968*exp(-0.005492*x)+0.005242*exp(-2.896*x)

Correlation parameter:

Error sum of squares: 0.0497,

Related coefficient: 0.9952

Degree of freedom: 712

Related coefficient after correction: 0.9952

Root-mean-square error: 0.0084

Fig. 8 shows the different interval acquired results difference analysis of embodiment 2, and comprise the matched curve of sedimentation kinetics equation, sedimentation kinetics equation is as follows:

fresult1(x)＝0.006717*exp(-2.916*x)+0.5178*exp(-0.01441*x)

fresult2(x)＝0.005319*exp(-3.094*x)+0.5085*exp(-0.01059*x)

fresult3(x)＝0.005011*exp(-3.008*x)+0.4991*exp(-0.006745*x)

fresult4(x)＝0.006114*exp(-3.019*x)+0.495*ex0(-0.004078*x)

fresult5(x)＝0.006858*exp(-3.051*x)+0.4947*exp(-0.002937*x)

fresult6(x)＝0.008867*exp(-3.035*x)+0.4928*exp(-3.774e-05*x)

fresult7(x)＝0.007669*exp(-2.843*x)+0.5083*exp(-0.008014*x)；

                                                             

Fig. 9 shows three typical images and characteristic curve thereof in the settling process of embodiment 3;

Figure 10 shows the settling characteristics information time sequence three-dimension curved surface of embodiment 3;

Figure 11 shows the sedimentation boundary after the settling characteristics information extraction of embodiment 3 and detects;

Figure 12 shows the sedimentation boundary defining method of embodiment 3;

Figure 13 shows the sedimentation kinetics equation matched curve of embodiment 3, and the sedimentation kinetics equation of embodiment 3 is as follows:

Fit equation:

fresult＝0.4086*exp(0.01479*x)+0.00389*exp(-5.533*x)

Correlation parameter:

Error sum of squares: 0.0424,

Related coefficient: 0.9937

Degree of freedom: 197

Related coefficient after correction: 0.9936

Root-mean-square error: 0.0147;

Figure 14 shows three typical images and characteristic curve thereof in the settling process of embodiment 4;

Figure 15 shows the settling characteristics information time sequence three-dimension curved surface of embodiment 4;

Figure 16 shows the sedimentation boundary after the settling characteristics information extraction of embodiment 4 and detects;

Figure 17 shows the sedimentation boundary defining method of embodiment 4.

Embodiment

The present inventor is by extensive and deep research, obtain a kind of heterogeneous liquid settlement automatic monitoring method and device, use the automatic monitoring that method of the present utility model can realize heterogeneous liquid sedimentation, quantitative evaluation can be carried out to the stability of suspending system, have that intellectuality, robotization, precision are high, sensitivity advantages of higher.

Of the present utility model one preferred embodiment in, the method for described monitoring heterogeneous liquid sedimentation comprises the following steps:

A () heterogeneous system to be measured loaded in sedimentation pipe fully shakes up after, put into monitoring device, the parameters such as light-source brightness, focal length, enlargement factor are adjusted to optimum efficiency;

(b) programmable interval, image acquisition quantity;

C () gathers the uniformity coefficient characteristic information of target area at setting-up time point;

D () sets up sedimentation dynamics surface model;

E interference region is rejected in the compacting of () threshold value, segmentation, extract sedimentation boundary eigenwert and obtain sedimentation dynamics point set data;

F () numerical fitting exports sedimentation kinetics equation and sedimentation kinetic curve.

Of the present utility model one preferred embodiment in, the settlement automatic monitoring equipment of described heterogeneous liquid comprises: imaging system, environmental control system and quantitative kinetic analysis system.

(1) imaging system described in comprises light source module and image capture module.

Light source module provides stable, even, adjustable illumination condition for sedimentation dynamics image information collecting process, thus obtaining high quality graphic data, described light-source system should meet following requirement: plane, cold light source, stable, anti-interference, brightness is adjustable, homogenous diffusion light type; Brightness is high, the life-span is long, and heating less, temperature is low, light is soft natural in stroboscopic, energy saving, easy for installation.Described light-source system be selected from the micro-light source of LED, LED array light source, LED area light source, shadowless lamp, cold light source bulb (xenon lamp), laser diode, gas laser one or more, and be not limited only to these, preferred LED micro-light source time not high for accuracy requirement; For in laser-induced fluorescence (LIF) test, preferred laser diode.

Described image capture module, for gathering settling characteristics information, comprises image acquisition detector and image acquisition control system.

Described image acquisition detector should meet following requirement: large panel CMOS or CCD panel, high resolving power 10 ~ 15M, programmability, focal length, brightness, contrast, white balance, enlargement factor can program calling and controlling, low distortion, can long-time stable collection; Self-scanning, high sensitivity, lightweight, volume is little, location of pixels is accurate, little power consumption, life-span length, good reliability, convenient signal treatment, rich interface be easy to programming.Described detector be selected from line array CCD, area array CCD, industrial CCD/CMOS, high resolution CCD/CMOS camera, fluorescence probe (coordinate with fluorescer use) one or more.

Technical grade image acquisition detector good stability, each photosensitive unit of CMOS photo-sensitive cell is independently, and also can not have influence on other unit even if damage, therefore price is lower; Secondly, because each unit independently carries out signal amplification and analog/digital conversion, therefore not only power consumption is very low, and the volume of whole module is also less.But be exaggerated device due to part larger in photosensitive unit and A/D converter occupies, the aperture opening ratio of traditional cmos element is very low, causes light utilization ratio poor thus; In addition, because each unit independently exports, the magnification of initialize signal is difficult to accomplish strict unification, and therefore the noise problem of image is more serious.

The advantage of line array CCD is that one dimension pixel number can do a lot, and total pixel number angle is few compared with area array CCD camera, and pixel dimension is relatively more flexible, and frame width number is high, is specially adapted to the measurement of one dimension dynamic object.And line array CCD resolving power is high, cheap, the requirement that great majority measure visual field can be met, but obtain two dimensional image with line array CCD, scanning motion must be equipped with, and in order to the correspondence position of each pixel of image on measured piece can be determined, the devices such as grating also must be equipped with to record the coordinate of each scan line of line array CCD.Supposedly, the requirement of these two aspects causes obtaining image with line array CCD following deficiency: image acquisition time is long, measures efficiency low; Due to the existence of scanning motion and corresponding position feedback element, add system complexity and cost; The precision of images may reduce by the impact of scanning motion precision, finally affects measuring accuracy.

The fluorescent characteristic that laser Induced Fluorescence Technology is excited by Study of Laser is come qualitative or is determined the character of test substances quantitatively.Because numerous fluorescence efficiencies that is organic and mineral compound own endogenous is low, in order to distinguish the different piece of various different sample or same sample, all kinds of fluorescence probe or fluorescent reagent and testing sample must be aided with and carry out specific binding.Following factor is mainly considered: excitation wavelength and fluorescence emission wavelengths during selection; Light stability and photobleaching; The quantitative and qualitative analysis of fluorescence; Toxicity; The pH environment be suitable for.

When, monitoring time less demanding to measuring accuracy is longer, preferred industrial cmos image detector; When measuring accuracy requires that high, monitoring time is shorter, preferred industrial CCD image detector.When measuring accuracy requires particle water white transparency in higher, testing sample, preferred fluorescence probe (coordinating fluorescer to use).

Described image acquisition control system, for regulating and controlling image acquisition detector, regulate optimal imaging parameter, set up the image-pickup method of high speed, high-resolution, low distortion, set up controlled settling process testing environment, ensure consistance, the repeatability of measurement result.

(2) environmental control system described in comprises temperature control module, standardization settling module, camera bellows alienation background module.

Described temperature control module is setting value for keeping sedimentation to measure environment temperature, can programmed control heating and cooling.Need meet the following conditions: temperature-15 ~ 100 DEG C of continuously adjustabe, temperature control sensitivity and error meet standard.Select the one in water-bath, metal bath, gas bath according to principle of work, bath temperature balance is fast, but temperature control scope is narrower, during water circulation flowing, can have an impact to imaging effect, meeting aggrandizement apparatus size; Metal bath adopts micro computer and semiconductor refrigerating/thermal technology to realize temperature control, utilizes metal module to heat, and warming and cooling rate is faster, and temperature control is accurate, and device also becomes exquisiter small and exquisite, and metal module need not add water, clean easy to clean; Gas bath is easy to use, but equalized temperature is slow, and accuracy of temperature control is lower.Therefore preferable alloy bath.

Described standardization settling module is for ensureing always controlled sedimentation environment, and core component is that sedimentation measures pipe, and sedimentation measures pipe (be preferably standard size) and should meet the following conditions: high transmission rate, low reflecting rate, to lead hot/cold performance good.Material is selected from acrylic, quartz glass, simple glass, phenolics, the one in silica gel, preferred quartz glass.

Described camera bellows alienation background module, for keeping environment in settlement monitoring process consistent, core component refers to differentiation background and camera bellows.For reducing later image analytical work amount, background need meet following requirement: primary colours are adjustable, the fine and smooth backboard of sub-light, aberration strengthen.Camera bellows is for keeping environment in settlement monitoring process consistent, and avoid extraneous light intensity to change, effect diagram is as collection effect; Keep box interior temperature constant, following requirement need be met: sealing, light tight, shielding external interference, insulation.Material is selected from: one or more in stainless steel, acrylic, iron, extruded polystyrene polyfoam, mold pressing type polystyrene foam.

(3) the image quantitative kinetic analysis system described in, comprising: visually strengthen module, characteristic extracting module, image analysis module, quantitative test module with result output module, image optimization.

Utilize visual with Output rusults module, setting image acquisition quantity, interval time, temperature, adjustment detector focal length, adjustment light intensity; Utilize image optimization to strengthen module, reduce the interference of noise, improve the image quality of image; Utilize characteristic extracting module, find out the edge feature of gray level image, determine liquid level separatrix, and then draw the level change in every width image; Utilize image analysis module, from time series sedimentation result, extract sedimentation boundary feature point set, set up sedimentation dynamics three-dimensional surface model; Utilize quantitative test module, sedimentation kinetics equation and subsidence curve can be obtained.The workflow of image quantitative kinetic analysis system is as follows:

Of the present utility model preferred embodiment in, the operation that described characteristic extracting module performs comprises:

1) image enhaucament: main target be image by image procossing, its visual quality of images is improved, be convenient to identify, improve the visual effect of image, make it be more suitable for the application of a certain specific occasion than original image, be convenient to for computer disposal.According to the difference of processing domain, image enhaucament is mainly divided into two kinds of methods: Space domain and frequency domain method.

2) filtering: object is to extract echo signal from noise.When the frequency spectrum of signal and noise does not overlap each other, bandpass filter is separated, otherwise the method for Wiener filtering need be adopted to obtain the Linear Minimum Mean-Square Error Estimation of signal.Test wavelet transformation, wavelet soft-threshold, based on noise-reduction methods such as mathematical morphology, neural network theory, fuzzy theory, Anisotropic diffusion equation, high-order statistic and the wave filters that utilizes Image Priori Knowledge to design.

3) limb recognition: the extractive technique at edge is a gordian technique in sedimentation image procossing, Roberts operator, Kirsch operator are for detecting the edge of step change type, Laplacian operator is for detecting the picture element of ridge or edge effect type, threshold value responsive type Sobel and Prewitt operator have stronger noise resisting ability, and Blurring edge detector realizes non-threshold values and detects.

4) Iamge Segmentation: conventional dividing method is mainly divided into the dividing method (threshold method, region-growing method, split degree method) based on region, the dividing method (Robert operator, Sobel operator, Pre-witt operator, Canny operator, Laplace operator, method of differential operator, frontier tracing method) based on edge and mixed type (genetic algorithm, wavelet transformation, Snake model).

Major advantage of the present utility model is:

(1) heterogeneous liquid settlement automatic monitoring equipment of the present utility model, can the monitoring heterogeneous liquid sedimentation of robotization, can monitor continuously sample, highly sensitive, monitoring and evaluation can be carried out to different settling behavior (rising, sinking, layering etc.), multiple sample can be monitored simultaneously, structure is simple, be convenient to safeguard;

(2) heterogeneous liquid settlement automatic monitoring method of the present utility model, favorable reproducibility, the measurement result that digital image-wise is preserved records settling process truly, facilitate subsequent analysis, can review, can not because of the storage of image, transmit or a series of map function such as to copy and change, reprocessing and in-depth analysis can be carried out to history testing result at any time; Processing accuracy is high, dirigibility is high, with the settling process information that digital image form is preserved, coordinate image optimization, analytical technology high-level efficiency can complete feature information extraction, modeling analysis, have compared with Traditional Man metering system save time, continuously, reliably, the advantage such as accurate.

Below in conjunction with specific embodiment, state the utility model further.Should be understood that these embodiments are only not used in restriction scope of the present utility model for illustration of the utility model.The experimental technique of unreceipted detailed conditions in the following example, conveniently condition is carried out usually, or according to the condition that manufacturer advises.

Embodiment 1 heterogeneous liquid settlement automatic monitoring equipment

As shown in Figure 1A, the settlement automatic monitoring equipment of the heterogeneous liquid in the present embodiment, comprising:

Sample bearing device 1, described sample bearing device 1 is for laying the container of splendid attire testing sample, and wherein said testing sample is the heterogeneous liquid that sedimentation can occur; Image collecting device 2, described image collecting device 2 is set to the image information for gathering described sample; Control device 3, described control device 3 is set to the work for controlling described image collecting device 2; With data processing equipment 4, described data processing equipment 4 is set to the liquid level separatrix for identifying in described image information, and identifies liquid level boundary line further over time.Data processing equipment described in the present embodiment is computing machine.

Image collecting device 2 is set to the image in different time points collected specimens, obtains the sample image information comprising many hardwoods image.The image information of described sample can be intensity of reflected light or the fluorescence intensity of sample.

Sample bearing device 1 can lay the container 11 of one or more (as 2,3,4,5,6,7,8,9,10) splendid attire testing sample, the sample bearing device 1 shown in Figure 1B can lay the container 11 of 6 splendid attire testing samples simultaneously, and detects at same batch.

Data processing equipment 4 comprises data processing module 41 and result derives module 42, and described data processing module 41 for the treatment of the image information collected, and stores brightness value, positional information and temporal information; Described result derives module 42 for deriving sedimentation kinetics equation and drawing subsidence curve.

As shown in Figure 1B, the settlement automatic monitoring equipment of the heterogeneous liquid in the present embodiment also comprises casing 6 and light-emitting device 5, described sample bearing device 1, described image collecting device 2 and described light-emitting device 5 are placed in described casing 6, and described light-emitting device 5 provides light source for described image collecting device 2; The luminous intensity of described light-emitting device 5 is adjustable, can be regulated the luminous intensity of light-emitting device 5 by control device 3.In the both sides of light-emitting device 5, reflector 51 can be set.

The light source of light-emitting device 5 can be: the micro-light source of LED, LED array light source, LED area light source, shadowless lamp, cold light source bulb (xenon lamp), laser diode and gas laser etc.

Equipment in the present embodiment also comprises temperature control equipment 7, and described temperature control equipment 7 is set to the temperature for regulating testing sample.Described temperature control equipment 7 can adopt water-bath, metal bath or gas bath to control described sample temperature.

Described control device 3 can also control temperature regulating device 7 and light-emitting device 5, can set and/or change the picture-taken frequency of described image collecting device 2, image acquisition quantity; Set and/or change the target temperature of described temperature control equipment 7; Set and/or change light intensity and/or the light application time of described light-emitting device 5.Control device 3 can comprise display panel, on a display panel the occurrence of display setting.

Described sample bearing device 1 comprises the support 12 of liquid container 11 and fixing described liquid container 11.

Support 12 can be common test-tube stent, also the form shown in Fig. 1 D, G can be arranged to, support 12 is provided with the hole 121 corresponding with described liquid container 11, described liquid container 11 is placed on described support 12 by described hole 121, described support is provided with longitudinal window 122, when the described liquid container 11 that testing sample is housed is placed on described support 12, described image collecting device 2 gathers the image information of described sample through described window 122.Liquid container 11 described in the present embodiment is transparent or semitransparent flat based tubes.

The material of liquid container 11 can be acrylic, quartz glass, simple glass, phenolics and silica gel, is preferably quartz glass.

Described image collecting device 2 comprises image acquisition device 21 and guide rail 22, and described image acquisition device 21 is movably arranged on described guide rail 22.In the present embodiment, be provided with two guide rails, the position of image acquisition device 21 can be moved back and forth or left and right, to obtain best image capture position.

Image acquisition device 21 can be selected from: line array CCD, area array CCD, industrial CCD/CMOS, high resolution CCD/CMOS and fluorescent probe.

In the present embodiment, described support 12 is metal support (being preferably copper, aldary, aluminum or aluminum alloy support), and described temperature control equipment 3 controls the temperature of described sample to be tested by described metal support 12.As referring to figure 1e, temperature control equipment 7 comprises temperature sensor (not shown), heating radiator 71 and well heater 72.Well heater 72 is placed in the below of support 12.In the present embodiment, casing 6 is camera bellows, and can arrange ventilating opening on casing 6, heating radiator 71 is relative with the ventilating opening on casing 6.

In other embodiments, if adopt common support (as test tube rack), background board can be set after support.

Embodiment 2 settling behavior is monitored

Sedimentation (downwards) behavior of sample (microcrystalline cellulose and colloidal microcrystalline cellulose mix by a certain percentage) in monitoring standard test tube (flat based tubes).Utilize this experimental provision and method to monitor, accurate subsidence curve and sedimentation kinetics equation can be obtained.

Step 1: use the heterogeneous liquid settlement automatic monitoring device in embodiment 1, testing liquid is loaded in flat based tubes, adjustment light-source brightness is optimal imaging effect; Set image acquisition interval, collecting quantity, working procedure.

Step 2: composition graphs 2, after collection terminates, read in whole picture, carry out automated graphics process (gray processing), extract the uniformity coefficient characteristic information (comprising test tube and background, interface, clarifying area, sedimentation boundary and the luminance brightness brightness value between white suspendible part and test tube) of target area (i.e. flat based tubes) in every pictures, draw Line profile (characteristic curve), between background area and flat based tubes, suspended matter all there is sudden change in brightness value.

Analyzing line profile known, there is sudden change and trough a place in liquid level position and flat based tubes upper end brightness value.Due to the accumulation of suspended matter in settling process, density increases, and causes brightness value to increase.Estimate so-called clarifying area in sample and also there is solid particle, therefore cause curve brightness value slowly to increase continuously, until during the sedimentation face of arrival, advance the speed and occur sudden change and b place.

C region between distance about 300-540 pixel is suspendible region (that is, the height of suspended matter), due to the instability of the condition such as illumination condition, CCD, causes the brightness value in c region to occur fluctuation.

Due to image can be made during CCD imaging to distort, to decline fast region so there is brightness value between region c, d, d is suspendible sections bottom position, and e is bottom test tube, and f is black background region.

Step 3: form the three-dimensional sedimentation dynamics surface model comprising temporal information after being merged according to image acquisition time by all characteristic curvees (in Fig. 3 curve), as shown in Figure 4, Fig. 4 shows settling characteristics information time sequence three-dimension curved surface.Fig. 4 and picture target area uniformity coefficient characteristic information stack result in time.Along with the carrying out of settling process, Line profile (sedimentation interphase) turns right skew gradually.By analyzing this this three-dimensional sedimentation dynamics surface model, the sedimentation dynamic information of heterogeneous liquid sample namely can be obtained.

Step 4: Fig. 5 be from the three-dimensional sedimentation dynamics surface model of Fig. 4 obtain about position and time 2-D sedimentation information, reflected out the position of sedimentation boundary (yellow curve) over time.Composition graphs 5, after in test tube, sample sedimentation completes, highs and lows place (target area is the white suspendible part in Fig. 3), by appearance two stable interphases, (these two interfacial brightness values are similar in suspendible region, two yellow area shown in Fig. 5): left side yellow area is sedimentation boundary, and the right yellow area is bottom interference region (two yellow area have identical brightness value).In subsequent treatment, by the mode of interception position, bottom interference region can be removed (X1=520).

Step 5: Fig. 6 is the two-dimentional sedimentation information about position and brightness value obtained from the three-dimensional sedimentation dynamics surface model of Fig. 4, and can reflect the brightness value at sedimentation boundary place, that is, the brightness value at sedimentation boundary place is in g region.Composition graphs 6, (in g region, brightness value span is 85-165 to select appropriate brightness scope, for making matched curve more accurate, choosing brightness value is 118-119 scope), remove the region of brightness value not in selected scope in Figure 5, obtain sedimentation boundary key feature point set data (the discontinuous curve of the green in Fig. 7), sedimentation dynamics matched curve (Fig. 7) and equation can be obtained through matching.

In the present embodiment, different brightness value spans is verified, as shown in Figure 8, respectively to different intervals (in the g interval of Fig. 6) in figure, brightness value is got the sedimentation kinetics equation that 90-100,100-110,110-120,120-130,130-140,140-150,90-150 obtain respectively and is corresponded respectively to fresult1 (x), fresult2 (x), fresult3 (x), fresult4 (x), fresult5 (x), fresult6 (x), fresult7 (x).Can draw from figure, in the g interval range of Fig. 6, get different range of luminance values, sedimentation kinetics equation matched curve (subsidence curve) difference obtained is very little, illustrates that method of the present utility model has good stability and reappearance.

Embodiment 3 monitors sedimentation (upwards) behavior of pik woods breast in cillin bottle

Sedimentation (upwards) behavior of pik woods breast in monitoring cillin bottle.This sample sedimentation fast (sedimentation completes near 200s), sample size few (putting into cillin bottle effective dimensions is 150 pixels), liquid level sedimentation change is little, and artificial reading cannot obtain subsidence curve accurately.Therefore utilize this experimental provision and method to monitor, after difference conversion, conventional subsidence curve (successively decreasing gradually) and sedimentation kinetics equation thereof can be obtained.

Step 1: use the heterogeneous liquid settlement automatic monitoring device in embodiment 1, testing liquid is loaded in flat based tubes, adjustment light-source brightness is optimal imaging effect; Set the time interval, collecting quantity, working procedure.

Step 2: composition graphs 9,10,11,12, draws settling characteristics information time sequence three-dimension curved surface.The stack result of Figure 10 and picture target area uniformity coefficient characteristic information.

Step 3: in conjunction with Figure 11, after in test tube, sample sedimentation completes, target area is at highs and lows place by appearance two stable interphases, and two yellow area: right area is sedimentation boundary, left area is upper end interference region.Choose suitable parameters to be removed.

Step 4: in conjunction with Figure 12,13, selects appropriate brightness scope, can remove interference region, can obtain sedimentation kinetics equation and matched curve.

Embodiment 4 monitors the settling behavior of different prescription pik woods breast in cillin bottle

The settling behavior (layering) of different prescription pik woods breast in monitoring cillin bottle, this sample not have obvious upwards or downward sedimentation boundary change procedure, belong to lamination.Utilize this experimental provision and method, correct time when sample realizes layering can be obtained.

Step 1: use the heterogeneous liquid settlement automatic monitoring device in embodiment 1, testing liquid is loaded in flat based tubes, adjustment light-source brightness is optimal imaging effect; Set the time interval, collecting quantity, working procedure.

Step 2: in conjunction with Figure 14,15,16,17, draws settling characteristics information time sequence three-dimension curved surface.The stack result of Figure 15 and picture target area uniformity coefficient characteristic information.As shown in figure 16, sample does not significantly rise or downtrending, belongs to lamination.The matrix obtained is analyzed, obtains the data point that derivative maximal value is corresponding, and then the correct time that sample starts to occur layering can be obtained.

The all documents mentioned at the utility model are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the utility model, and these equivalent form of values fall within the application's appended claims limited range equally after having read above-mentioned instruction content of the present utility model.

Claims (11)

1. a settlement automatic monitoring equipment for heterogeneous liquid, is characterized in that, described equipment comprises:

Sample bearing device, described sample bearing device is set to the container for laying splendid attire testing sample, and wherein said testing sample is the heterogeneous liquid that sedimentation can occur;

Image collecting device, described image collecting device is set to the image information for gathering described sample;

Control device, described control device is set to the work for controlling described image collecting device; With

Optional data processing equipment, described data processing equipment is set to the liquid level separatrix for identifying in described image information, and/or identifies liquid level boundary line further over time.

2. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 1, is characterized in that, the image that described image collecting device is arranged in use in different time points collected specimens obtains the sample image information comprising many hardwoods image.

3. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 1, it is characterized in that, the settlement automatic monitoring equipment of described heterogeneous liquid also comprises casing and light-emitting device, described sample bearing device, described image collecting device and described light-emitting device are placed in described casing, and described light-emitting device provides light source for described image collecting device.

4. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 1, it is characterized in that, the settlement automatic monitoring equipment of described heterogeneous liquid also comprises temperature control equipment, and described temperature control equipment is set to the temperature for regulating sample.

5. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 1, is characterized in that, described sample bearing device comprises the support of liquid container and fixing described liquid container.

6. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 1, it is characterized in that, described image collecting device comprises image acquisition device and guide rail, and described image acquisition device is movably arranged on described guide rail.

7. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 1, it is characterized in that, described data processing equipment comprises data processing module and result derives module, described data processing module for the treatment of the image information collected, and stores brightness value, positional information and temporal information; Described result derives module for deriving sedimentation kinetics equation and drawing subsidence curve.

8. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 3, it is characterized in that, described control device is also for controlling described light-emitting device.

9. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 4, it is characterized in that, described control device is also for controlling described temperature control equipment.

10. the settlement automatic monitoring equipment of heterogeneous liquid as claimed in claim 5, it is characterized in that, described support is provided with the hole corresponding with described liquid container, described liquid container is laid on the bracket by described hole, described support is provided with longitudinal window, when the described liquid container that testing sample is housed is laid on the bracket, described image collecting device gathers the image information of described sample through described window.

The settlement automatic monitoring equipment of 11. heterogeneous liquid as claimed in claim 1, it is characterized in that, described image collecting device comprises image acquisition device and guide rail, and described image acquisition device is movably arranged on described guide rail.