CN204731161U - A kind of ammonia nitrogen fluorescence detection device - Google Patents
A kind of ammonia nitrogen fluorescence detection device Download PDFInfo
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- CN204731161U CN204731161U CN201520418497.9U CN201520418497U CN204731161U CN 204731161 U CN204731161 U CN 204731161U CN 201520418497 U CN201520418497 U CN 201520418497U CN 204731161 U CN204731161 U CN 204731161U
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- bottle
- ammonia
- reaction film
- generates
- ammonia nitrogen
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Abstract
The utility model discloses a kind of ammonia nitrogen fluorescence detection device, comprise ammonia and generate bottle, reaction film frame and detect bottle, it is wide-necked bottle that described ammonia generates bottle; Described reaction film frame comprises the mounting table and hanger bracket that middle part is through hole, and described mounting table and hanger bracket are connected as a single entity; Described detection bottle is fitted with ultra violet light emitter, and the side detecting bottle is provided with transparent observing window; The bottleneck that ammonia generates bottle is communicated with the bottom detecting bottle, and reaction film frame is set in ammonia and generates bottle and the junction detecting bottle.This device is convenient for carrying, and operates simple and easy, and layman can operate, and adapts to wide; Only need be compared with standard color comparison card by eye observation reaction film shade, the concentration of ammonia nitrogen in water sample can be judged, water sample without the need to filtering, easy quick, accuracy is higher.
Description
Technical field
The utility model relates to water body ammonia nitrogen detection field, especially a kind of ammonia nitrogen fluorescence detection device.
Background technology
Ammonia nitrogen is the nutriment of algae substances, and can make water body generation eutrophication, thus make water hypoxia, water quality deterioration, the hydrobionts such as fish cannot survive.Meanwhile, under aerobic conditions, ammonia nitrogen can be nitrite nitrogen by microbiological oxidation, and nitrite nitrogen and protein bound generate nitrosamine, have carcinogenic and teratogenesis.Therefore, in water body, ammonia nitrogen concentration can reflect the contaminated degree of water body, is that surface water quality and fishery water water quality must survey one of index.
The ammonia nitrogen detection method reported has gassensing ammonia electrode method, visible spectrophotometry and fluorescent spectrometry etc., and these methods need instrument and equipment and professional person's operation of specialty, are difficult to towards layman such as fishermen.
Utility model content
The purpose of this utility model is to provide a kind of ammonia nitrogen fluorescence detection device, and this device is convenient for carrying, and operates simple and easy, and layman can operate, and adapts to wide; Only need be compared with standard color comparison card by eye observation reaction film shade, the concentration of ammonia nitrogen in water sample can be judged, easy quick, accuracy is higher.
The technical scheme realizing the utility model object is:
A kind of ammonia nitrogen fluorescence detection device, comprises
Ammonia generates bottle, and for being ammonia by the mineralized nitrogen in water sample, it is wide-necked bottle that described ammonia generates bottle;
Reaction film frame, for placing response film, described reaction film frame comprises the mounting table and hanger bracket that middle part is through hole, and described mounting table and hanger bracket are connected as a single entity;
Detect bottle, for the shade of the fluorescence that observing response film sends, described detection bottle is fitted with ultra violet light emitter, and the side of described detection bottle is provided with transparent observing window;
The bottleneck that described ammonia generates bottle is communicated with the bottom detecting bottle, and reaction film frame is set in ammonia and generates bottle and the junction detecting bottle.
Described mounting table and hanger bracket are screwed by screw thread, are spirally connected and are connected as a single entity.
Ammonia generates the bottleneck band external thread of bottle, and detect bottle and generates the internal thread of bottle adaptation with ammonia, the bottleneck that described ammonia generates bottle is threaded connection with the bottom of detection bottle.
Ammonia nitrogen in water sample comprises NH
4 +and NH
3two kinds of forms.
Adopt the ammonia nitrogen fluorescence detection method of said apparatus, comprise the steps:
1) get 50 mL water samples from sample seedbed and be placed in ammonia generation bottle, add 5mL buffer solution;
2) getting a reaction film is placed on the mounting table of reaction film frame, detection bottle and ammonia is generated bottle and screws and be connected, and detects bottle in upper vertical placement;
3) yawing pick-up unit gently, the reactant liquor of mixing step 1), leaves standstill 2-3 hour, ammonia and reaction film is fully reacted;
4) ultra violet light emitter is opened, from the color of watch window observing response film, reference standard colorimetric card, the concentration of answering with the immediate Color pair of this reaction film in standard color comparison card is the ammonia nitrogen concentration value of this water sample;
The preparation of described buffer solution: pack 1 is dissolved in 50 mL pure water formulated; Described pack 1 is that 2.6 g disodium ethylene diamine tetraacetates (EDTA) and 1.0 g NaOH solids mixings form;
The preparation of described reaction film comprises:
A) reaction film soak solution: pack 2 is dissolved in 50 mL pure water, is mixed with reaction film soak solution; Described pack 2 is the o-phthalaldehyde(OPA) (OPA) of 0.03975g and the Na of 0.0125g
2sO
3solids mixing forms;
B) reaction film makes: the little filter paper of circle quantitative filter paper being cut into diameter 32 mm, is placed in reaction film soak solution and soaks 15 min-120 min, makes filter paper fully absorb reaction reagent OPA and Na in soak solution
2sO
3;
The preparation of described standard color comparison card comprises:
A) prepare each 50 mL of serial Ammonia nitrogen standard liquid within the scope of 2-32 μm of ol/L, the ammonia pouring pick-up unit respectively into generates in bottle, adds 5 mL buffer solution;
B) getting a reaction film is placed on the mounting table of reaction film frame, detection bottle and ammonia is generated bottle and screws and be connected, and detects bottle in upper vertical placement;
C) yawing pick-up unit gently, mixing steps A) reactant liquor, leave standstill 2-3 hour, ammonia and reaction film fully reacted;
D) ultra violet light emitter is opened, the color of the reaction film of the standard solution of variable concentrations is observed from watch window, and the color color lump of the reaction film of the standard solution of each variable concentrations observed is drawn out in contrast, then the standard color block these being represented variable concentrations is printed on colorimetric card carrier, carries out corresponding concentration value mark to each standard color block.
Judge the concentration of water sample: open ultra violet light emitter, from the color of watch window observing response film, reference standard colorimetric card, the concentration of answering with the immediate Color pair of this reaction film in standard color comparison card is the concentration of this water sample, if reaction film color is between standard color comparison card two concentration, can estimates between and read a concentration.
Principle of the present utility model: be NH by the mineralized nitrogen in water sample
3, and immersed with o-phthalaldehyde(OPA) (OPA) and Na
2sO
3the filter paper of reagent and the reaction of reaction film generation solid phase fluorescent, under the irradiation of uviol lamp, the fluorescence color depth that reaction film sends is relevant with ammonia nitrogen concentration in water sample.
This device is convenient for carrying, and operates simple and easy, and layman can operate, and adapts to wide; This method only need be compared with standard color comparison card by eye observation reaction film shade, can judge the concentration of ammonia nitrogen in water sample, water sample without the need to filtering, easy fast, accuracy is higher.
Accompanying drawing explanation
Fig. 1 is the structural representation of ammonia nitrogen fluorescence detection device in embodiment;
Fig. 2 is the structural representation of reaction film frame in embodiment;
Fig. 3 is embodiment Plays colorimetric card schematic diagram.
In figure, 1. ammonia generates bottle 2. mounting table 3. hanger bracket 4. and detects bottle 5. ultra violet light emitter 6. watch window 7. through hole.
Embodiment
Below in conjunction with drawings and Examples, the utility model content is further elaborated, but is not to restriction of the present utility model.
Embodiment:
With reference to Fig. 1 Fig. 2, a kind of ammonia nitrogen fluorescence detection device, comprises
Ammonia generates bottle 1, and for being ammonia by the mineralized nitrogen in water sample, it is wide-necked bottle that described ammonia generates bottle 1;
Reaction film frame, for placing immersed with o-phthalaldehyde(OPA) and Na
2sO
3the filter paper of reagent and reaction film, described reaction film frame comprises the mounting table 2 and hanger bracket 3 that middle part is through hole 7, and described mounting table 2 and hanger bracket 3 are connected as a single entity;
Detect bottle 4, for the shade of the fluorescence that observing response film sends, described detection bottle 4 is fitted with ultra violet light emitter 5, and the side of described detection bottle 4 is provided with transparent observing window 6;
The bottleneck that described ammonia generates bottle 1 is communicated with the bottom detecting bottle 4, and reaction film frame is set in ammonia and generates bottle 1 and the junction detecting bottle 4.
Described mounting table 2 and hanger bracket 3 are screwed by screw thread, are spirally connected and are connected as a single entity.
Ammonia generates the bottleneck band external thread of bottle 1, and detect bottle 4 and generates the internal thread of bottle 1 adaptation with ammonia, the bottleneck that described ammonia generates bottle 1 is threaded connection with the bottom of detection bottle 4.
Adopt the ammonia nitrogen fluorescence detection method of said apparatus, comprise the steps:
1) get 50 mL water samples from sample seedbed and be placed in ammonia generation bottle 1, add 5mL buffer solution;
2) getting a reaction film is placed on the mounting table 2 of reaction film frame, detection bottle 4 and ammonia is generated bottle 1 and screws and be connected, and detects bottle 4 in upper vertical placement;
3) yawing pick-up unit gently, the reactant liquor of mixing step 1), leaves standstill 2-3 hour, ammonia and reaction film is fully reacted;
4) ultra violet light emitter 5 is opened, from the color of watch window 6 observing response film, reference standard colorimetric card, the concentration of answering with the immediate Color pair of this reaction film in standard color comparison card is the ammonia nitrogen concentration value of this water sample;
The preparation of described buffer solution: pack 1 is dissolved in 50 mL pure water formulated; Described pack 1 is that 2.6 g disodium ethylene diamine tetraacetates (EDTA) and 1.0 g NaOH solids mixings form;
The preparation of described reaction film comprises:
A) reaction film soak solution: pack 2 is dissolved in 50 mL pure water, is mixed with reaction film soak solution; Described pack 2 is the o-phthalaldehyde(OPA) (OPA) of 0.03975g and the Na of 0.0125g
2sO
3solids mixing forms;
B) reaction film makes: the little filter paper of circle quantitative filter paper being cut into diameter 32 mm, is placed in reaction film soak solution and soaks 15 min-120 min, makes filter paper fully absorb reaction reagent OPA and Na in soak solution
2sO
3;
The preparation of described standard color comparison card comprises:
A) prepare each 50 mL of serial Ammonia nitrogen standard liquid within the scope of 2-32 μm of ol/L, the ammonia pouring pick-up unit respectively into generates in bottle, adds 5 mL buffer solution;
B) getting a reaction film is placed on the mounting table 2 of reaction film frame, detection bottle 4 and ammonia is generated bottle 1 and screws and be connected, and detects bottle 4 in upper vertical placement;
C) yawing pick-up unit gently, mixing steps A) reactant liquor, leave standstill 2-3 hour, ammonia and reaction film fully reacted;
D) ultra violet light emitter 5 is opened, the color of the reaction film of the standard solution of variable concentrations is observed from watch window 6, and the color color lump of the reaction film of the standard solution of each variable concentrations observed is drawn out in contrast, then the standard color block these being represented variable concentrations is printed on colorimetric card carrier, carries out corresponding concentration value mark to each standard color block.Standard color comparison card color corresponding to different ammonia nitrogen concentration as shown in Figure 3.
Particularly, adopt this pick-up unit and method to determine and pick up from the upper of Lingchuan County, Guilin City Hua Jiang Guilin Electronic Science and Technology Univ. section, in, each 3 water samples in downstream, sampling time is on May 7th, 2015, and respectively by this pick-up unit and method, and indophenol blue spectrophotometric method measures, each replicate determination 3 times, result is as shown in table 1.
Table 1 water determination result
As can be seen from Table 1, concentration measured by these apparatus and method records result closely with indophenol blue method, difference is within 10%, visible apparatus and method can compared with the concentration of ammonia nitrogen in Accurate Determining water body, and compare indophenol blue method, this device is simple to operate, result is accurate, be convenient for carrying, water sample is without the need to filtering, and layman can operate.
Claims (2)
1. an ammonia nitrogen fluorescence detection device, is characterized in that, comprises
Ammonia generates bottle, and for being ammonia by the mineralized nitrogen in water sample, it is wide-necked bottle that described ammonia generates bottle;
Reaction film frame, for placing response film, described reaction film frame comprises the mounting table and hanger bracket that middle part is through hole, and described mounting table and hanger bracket are connected as a single entity;
Detect bottle, for the shade of the fluorescence that observing response film sends, described detection bottle is fitted with ultra violet light emitter, and the side of described detection bottle is provided with transparent observing window;
The bottleneck that described ammonia generates bottle is communicated with the bottom detecting bottle, and reaction film frame is set in ammonia and generates bottle and the junction detecting bottle.
2. ammonia nitrogen fluorescence detection device according to claim 1, is characterized in that, the bottleneck that described ammonia generates bottle is threaded connection with the bottom detecting bottle.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520418497.9U CN204731161U (en) | 2015-06-17 | 2015-06-17 | A kind of ammonia nitrogen fluorescence detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520418497.9U CN204731161U (en) | 2015-06-17 | 2015-06-17 | A kind of ammonia nitrogen fluorescence detection device |
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| Publication Number | Publication Date |
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ID=54389500
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880449A (en) * | 2015-06-17 | 2015-09-02 | 桂林电子科技大学 | Ammonia nitrogen fluorescence detection device and detection method |
-
2015
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880449A (en) * | 2015-06-17 | 2015-09-02 | 桂林电子科技大学 | Ammonia nitrogen fluorescence detection device and detection method |
| CN104880449B (en) * | 2015-06-17 | 2017-11-21 | 桂林电子科技大学 | A kind of ammonia nitrogen fluorescence detection method |
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|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151028 Termination date: 20170617 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |