CN204705520U - A kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device - Google Patents

A kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device Download PDF

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Publication number
CN204705520U
CN204705520U CN201520398862.4U CN201520398862U CN204705520U CN 204705520 U CN204705520 U CN 204705520U CN 201520398862 U CN201520398862 U CN 201520398862U CN 204705520 U CN204705520 U CN 204705520U
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China
Prior art keywords
fixed
guidance panel
adductor muscle
zero graduation
graduation line
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Expired - Fee Related
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CN201520398862.4U
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Chinese (zh)
Inventor
王悠
孙田力
唐学玺
周斌
姜永顺
董文隆
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Ocean University of China
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Ocean University of China
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Abstract

The utility model relates to a kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, comprises guidance panel; Described guidance panel vertically arranges scale mark to the left from centre, and the scale mark at centre place is zero graduation line; Described guidance panel arranges fixed groove, and described zero graduation line is divided equally about fixed groove; The top of described fixed groove is provided with fixed transverse rod, and described fixed transverse rod two ends are fixed on described guidance panel by fixed leg; The one that the utility model provides is for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, improve blood sampling efficiency, the specification application function of utensil, reduce sampling working strength, can accurately locate closed shell flesh position without the need to cutting shell open and carry out getting blood operation, the structure of equipment is simple, designs ingenious, posterior adductor muscle accurate positioning, simple operation, efficient.

Description

A kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device
Technical field
The utility model relates to biological study equipment technical field, and particularly one is used for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device.
Background technology
The haemocyte of shellfish take part in the reparation of body injury, shell reconstruction, engulf foreign particles and eliminate the processes such as noxious material, be the main undertaker of shellfish immunity.Therefore, in shellfish immunological experiment, how to get the most most important previous step of fundamental sum that blood becomes scientific research.Shellfish gets the mode of blood primarily of two kinds, and namely cardiocoelom gets blood and closed shell flesh gets blood.Cardiocoelom is got blood and is often applicable to the larger shellfishes such as scallop, concerning with the compact seashells such as Mytilus galloprovincialis (test and be generally 4 ~ 6cm by shellfish specification), auricular position is difficult to determine, therefore the general posterior adductor muscle containing large blood sinus of selecting is as getting blood object.
The main method that traditional Mytilus galloprovincialis posterior adductor muscle gets blood is: first cut Mytilus galloprovincialis posterior adductor muscle with scalpel, open shell, then inserts closed shell intramuscular portion extraction hemolymph with the syringe needle of syringe.But classic method has the following disadvantages: the method that (1) incision Mytilus galloprovincialis posterior adductor muscle gets blood destroys the large blood sinus of closed shell intramuscular, certainly will cause a large amount of losses of hemolymph; Meanwhile, shrink after closed shell flesh muscle is cut-off, exacerbate exosmosing of hemolymph; The hemolymph amount now using syringe to extract is considerably less, needs to take a large amount of shellfishes to complete sampling work, considerably increases the intensity of sampling work; (2) because Mytilus galloprovincialis sexual gland positional distance posterior adductor muscle is nearer, often sexual gland is damaged in the process of closed shell flesh muscle after severance, cause exosmosing of reproduction cell (mainly sperm), mix with hemolymph, the purity of the blood sample that impact is extracted.Therefore, invent the demand that a kind of convenient, efficient, highly purified Mytilus galloprovincialis blood extracting method and device meet people, but also do not find about relevant report of the present invention so far.
Utility model content
For the defect existed in prior art, the purpose of this utility model is to provide
The utility model adopts following technical scheme:
A kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, comprise guidance panel;
Described guidance panel vertically arranges scale mark to the left from centre, and the scale mark at centre place is zero graduation line;
Described guidance panel arranges fixed groove, and described zero graduation line is divided equally about fixed groove;
The top of described fixed groove is provided with fixed transverse rod, and described fixed transverse rod two ends are fixed on described guidance panel by fixed leg;
The infall of described fixed transverse rod and described zero graduation line arranges triangle and identifies; Described fixed transverse rod arranges in the left side of triangle mark mark of sliding;
On guidance panel, zero graduation line both sides arrange two parallel sliding trays; A slide post is set in two sliding trays respectively, between two slide posts, is fixed with slip cross bar;
Between the fixed leg and slide post of zero graduation line homonymy, identical rubber strip is set; Described rubber strip arranges length mark; Described length mark accounts for 23% of rubber strip total length apart from the distance of fixed leg;
The intersection of described slip cross bar and zero graduation line arranges draw-in groove.
On the basis of such scheme, the width of described fixed groove is 1cm.
On the basis of such scheme, the width of described draw-in groove is 0.5cm.
The one that the utility model provides is for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, improve blood sampling efficiency, the specification application function of utensil, reduce sampling working strength, can accurately locate closed shell flesh position without the need to cutting shell open and carry out getting blood operation, the structure of equipment is simple, designs ingenious, posterior adductor muscle accurate positioning, simple operation, efficient.
Accompanying drawing explanation
The utility model has following accompanying drawing:
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the syndeton schematic diagram of fixed leg described in the utility model and slide post;
Fig. 3 is the structural representation of slide post described in the utility model and sliding tray.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Embodiment 1
As shown in figures 1 and 3, a kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, comprise guidance panel 1; Described guidance panel 1 vertically arranges scale mark 2 to the left from centre, and the scale mark 2 at centre place is zero graduation line 13, is followed successively by left: " 5 ", " 10 ", " 15 ", " 20 ", " 25 ", " 30 ", " 35 ", " 40 "; Described guidance panel 1 arranges fixed groove 3, and fixed groove about 3 is divided equally by described zero graduation line 13; The top of described fixed groove 3 is provided with fixed transverse rod 4, and described fixed transverse rod 4 two ends are fixed on described guidance panel 1 by fixed leg 7; Described fixed transverse rod 4 arranges triangle with the infall of described zero graduation line 13 and identifies 5; Described fixed transverse rod 4 arranges slip mark 6 in the left side of triangle mark 5; On guidance panel 1, zero graduation line 13 both sides arrange two parallel sliding trays 12; Arrange respectively in two sliding trays 12 between a slide post 11, two slide posts 11 and be fixed with slip cross bar 9; Described slip cross bar 9 arranges draw-in groove 10 with the intersection of zero graduation line 13.
As depicted in figs. 1 and 2, identical rubber strip 8 is set between the fixed leg 7 and slide post 11 of zero graduation line 13 homonymy; Described rubber strip 8 arranges length mark 14; Described length mark 14 accounts for 23% of rubber strip 8 total length apart from the distance of fixed leg 7; Preferably, the width of described fixed groove 3 is 1cm.Preferably, the width of described draw-in groove 10 is 0.5cm.
In textbook to Mytilus galloprovincialis posterior adductor muscle to be described as flesh trace large, oval, be positioned at the slightly biased back of the body edge in shell rear end.But comparatively general to the definition of Mytilus galloprovincialis posterior adductor muscle position in book, beginner is often difficult to accurate location, even needs to dissect the position that a large amount of Mytilus galloprovincialis practises determining posterior adductor muscle place.Location the utility model for posterior adductor muscle adopts the mode of coordinate axis location to determine, fixed transverse rod 4 is decided to be abscissa axis, " 0 " scale mark is decided to be axis of ordinates, unit is mm.Upwards to be vertically placed on the left of Mytilus galloprovincialis in fixed groove 3 and top apex is in the draw-in groove 10 of slip cross bar 9 time, Mytilus galloprovincialis is divided into left and right two parts through top apex by " 0 " scale mark, Mytilus galloprovincialis posterior adductor muscle is positioned at 1/2 place of abscissa axis " 0 " scale mark to left side the widest part vertical range, axis of ordinates is that Mytilus galloprovincialis is totally long near horizontal ordinate axle head 23% place, is the position at posterior adductor muscle place by the joint of above two groups of data bus connections on shell.
Posterior adductor muscle puncture blood extracting method step:
1, inserting shell with sharp mouth tweezers from the Mytilus galloprovincialis byssus gland place for getting blood inner, shell being pried open a gap gently, the seawater in shell is flowed out;
2, pull slip cross bar 9 downwards, will upwards be vertically placed in fixed groove 3 on the left of Mytilus galloprovincialis, Mytilus galloprovincialis be fixed between fixed transverse rod 4 and slip cross bar 9, and top apex is in the draw-in groove 10 of slip cross bar 9;
3, read the scale of the widest part on the left of shell, calculate the scale of the widest part to 1/2 place of " 0 " scale mark vertical range, and the slip mark 6 of sliding on fixed transverse rod 4 is as mark;
4, to identify the scale mark of 6 for the longitudinal axis through the slip on fixed transverse rod 4, with the line of the length mark 14 on two rubber strips 8 for transverse axis, the position of diaxon point of crossing on shell is determined;
5, bore with miniature electric-torch shell polishing in point of crossing place directly can be inserted get blood (directly not punching shell with electric drill, in order to avoid damage closed shell flesh) to being got blood pin;
6, a hand-held blood collection needles, vertically inserts in closed shell flesh from shell burnishing part, and another hand slowly pulls syringe to get blood, and the hand holding blood collection needles can control syringe needle and move up and down, and also can tilt slightly towards surrounding, to obtain more hemolymph.
Specific embodiment of the utility model is only the object for exemplary illustration; it limits protection scope of the present invention never in any form; those skilled in the art can be improved according to the above description or be converted, and all these improve and convert the protection domain that all should belong to the utility model claims.

Claims (3)

1., for a Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, it is characterized in that, comprise guidance panel (1);
Described guidance panel (1) vertically arranges scale mark (2) to the left from centre, and the scale mark (2) at centre place is zero graduation line (13);
Described guidance panel (1) arranges fixed groove (3), and fixed groove (3) left and right is divided equally by described zero graduation line (13);
The top of described fixed groove (3) is provided with fixed transverse rod (4), and described fixed transverse rod (4) two ends are fixed on described guidance panel (1) by fixed leg (7);
Described fixed transverse rod (4) arranges triangle with the infall of described zero graduation line (13) and identifies (5); Described fixed transverse rod (4) arranges in the left side of triangle mark (5) mark (6) of sliding;
Two parallel sliding trays (12) are set in upper zero graduation line (13) both sides of guidance panel (1); A slide post (11) is set in two sliding trays (12) respectively, between two slide posts (11), is fixed with slip cross bar (9);
Be positioned between the fixed leg (7) of zero graduation line (13) homonymy and slide post (11) and identical rubber strip (8) is set; Described rubber strip (8) is arranged length mark (14); Described length mark (14) accounts for 23% of rubber strip (8) total length apart from the distance of fixed leg (7);
Described slip cross bar (9) arranges draw-in groove (10) with the intersection of zero graduation line (13).
2. according to claim 1 for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, it is characterized in that, the width of described fixed groove (3) is 1cm.
3. according to claim 1 for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device, it is characterized in that, the width of described draw-in groove (10) is 0.5cm.
CN201520398862.4U 2015-06-10 2015-06-10 A kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device Expired - Fee Related CN204705520U (en)

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CN201520398862.4U CN204705520U (en) 2015-06-10 2015-06-10 A kind of for Mytilus galloprovincialis posterior adductor muscle puncture blood sampling device

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110794024A (en) * 2019-11-30 2020-02-14 中国海洋大学 Standardized determination method for shellfish blood physiological indexes
CN112608825A (en) * 2020-12-28 2021-04-06 浙江省海洋水产研究所 Visual mussel hemolymph collection system
CN112656997A (en) * 2020-12-28 2021-04-16 浙江省海洋水产研究所 Draw-out equipment convenient to gather mussel bloody lymph

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110794024A (en) * 2019-11-30 2020-02-14 中国海洋大学 Standardized determination method for shellfish blood physiological indexes
CN110794024B (en) * 2019-11-30 2022-05-10 中国海洋大学 Standardized determination method for shellfish blood physiological indexes
CN112608825A (en) * 2020-12-28 2021-04-06 浙江省海洋水产研究所 Visual mussel hemolymph collection system
CN112656997A (en) * 2020-12-28 2021-04-16 浙江省海洋水产研究所 Draw-out equipment convenient to gather mussel bloody lymph
CN112608825B (en) * 2020-12-28 2023-12-12 浙江省海洋水产研究所 Visual mussel blood lymph collection system
CN112656997B (en) * 2020-12-28 2024-04-09 浙江省海洋水产研究所 Extraction equipment convenient to gather mussel blood lymph

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CF01 Termination of patent right due to non-payment of annual fee
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Granted publication date: 20151014

Termination date: 20170610