CN204666416U - A kind of high flux check-out console for single cell gel electrophoresis test - Google Patents
A kind of high flux check-out console for single cell gel electrophoresis test Download PDFInfo
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- CN204666416U CN204666416U CN201320138382.5U CN201320138382U CN204666416U CN 204666416 U CN204666416 U CN 204666416U CN 201320138382 U CN201320138382 U CN 201320138382U CN 204666416 U CN204666416 U CN 204666416U
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- hole
- out console
- check
- high flux
- single cell
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Abstract
The utility model provides a kind of high flux check-out console for single cell gel electrophoresis test, it is characterized in that, comprise the plate body of high light-transmitting materials, and the several regular aperture be formed on described plate body, hole shape is square or circular, be planar bottom surface at the bottom of the hole in described hole, and hole is multiple rule, disconnected mutually, ensure that hole inner gel thickness is homogeneous.Compared to routine microslide once can only process a sample, this check-out console once can process multiple sample simultaneously, volley of rifle fire application of sample can be adopted, compared to classic method, avoid human factor and cause gel slab became uneven shortcoming, ensure that consistance prepared by gel film and repeatability, improve the quality of experiment.
Description
Technical field
The utility model relates to DNA electrophoretic techniques field, more particularly, relates to the high flux check-out console used in a kind of single cell gel electrophoresis process.
Background technology
Single cell gel electrophoresis graphical analysis (single cell gel electrophoresis, SCGE) technology, also known as comet assay method (comet assay), be a kind of research and simple, sensitive, the new technology fast quantitatively detecting individual cells DNA damage.Under Related Factors brings out, the impaired meeting of DNA of cell causes its higher structure to change, and causes its superhelix loose.Now, the DNA fragmentation of damage can shift out when electrophoresis from core, anode migration distribution in tail shape, and the cell portion of can't harm still keeps spherical.After fluorescent dye, can be observed comet formation pattern.Tail of a comet length and fluorescence power waits relevant with DNA damage degree, researchs and analyses basis in this, as SCGE technology.This technology has been widely used in genetic toxicology, occupation and environmental biological monitoring, radiobiology, oncology, Environmental ecology and apoptosis-related experimental study as DNA damage detection method at present.
Sample preparation basic step for single cell gel electrophoresis image analysis technology comprises: offset plate preparation, lysis and electrophoresis, neutralize and dye.Wherein the preparation of offset plate is very crucial.At present, the preparation method of widespread use is at sanding microslide upper berth gel.Between offset plate easy damaged and sample, homogeneity problem is that this tests the difficult problem always existed.In addition, microslide once can only process a sample, have impact on the propagation and employment of this technology in medicament high flux screening field.
Therefore, current in the urgent need to a kind of can improve offset plate quality and can high flux use single cell gel electrophoresis check-out console.
Utility model content
The purpose of this utility model is a kind of high flux check-out console for single cell gel electrophoresis test, it is characterized in that, a plate face of described check-out console has multiple rule, mutual disconnected aperture,
Wherein said hole is square hole, and the length of side is 0.8-1.2cm; Or
Wherein said hole is circular port, and radius is 0.4cm.
The plate body length of described plate is 3.5-12.5cm, and width is 3.5-8.5cm, and thickness is 0.1-0.5cm.Described hole is regular distribution.The quantity in described hole is 4-32.Described hole depth is 0.02 ~ 0.1cm.Described pitch-row panel edges 0.25-0.65cm.Described pitch of holes 0.4-0.8cm.Described sheet material matter is selected from glass, poly-third ethene or polypropylene.
The utility model provides a kind of single cell gel electrophoresis check-out console, it is characterized in that, comprise the plate body of high light-transmitting materials, and the several regular aperture be formed on described plate body, hole shape is square or circular, be planar bottom surface at the bottom of the hole in described hole, and hole is multiple rule, disconnected mutually, ensure that hole inner gel thickness is homogeneous.Compared to routine microslide once can only process a sample, this check-out console once can process multiple sample simultaneously, volley of rifle fire application of sample can be adopted, compared to classic method, avoid human factor and cause gel slab became uneven shortcoming, ensure that consistance prepared by gel film and repeatability, improve the quality of experiment.
By experiment, design produces a desired effect substantially.Multiple sample can be processed simultaneously, decrease repeated work, reach high-throughout object.Avoid human factor and cause gel slab became uneven shortcoming, improve consistance and repeatability prepared by gel film, decrease the impact of human factor, improve quality of experiments.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic top plan view of embodiment 1;
Fig. 2 is the one-piece construction schematic top plan view of embodiment 2;
Fig. 3 is the one-piece construction schematic top plan view of embodiment 3.
Embodiment
Below, embodiment of the present utility model is described in detail by reference to the accompanying drawings.
Embodiment 1
Plate body adopts organic glass material, and plate body length is 12.5cm, and width is 8.5cm, and thickness is 0.5cm; Plate body is made a call to 24 holes, and distribute in 6*4, hole is square, and the length of side is 1.2cm, is planar bottom surface at the bottom of hole.Pitch-row panel edges 0.65cm, pitch of holes is 0.8cm, hole depth 0.05cm.Shown in Figure 1.
Embodiment 2
Plate body adopts glass material, and plate body length is 3.5cm, and width is 3.5cm, and thickness is 0.5cm; Plate body is made a call to 4 holes, distributes in 2*2, hole is circular, and radius is 0.4cm, is planar bottom surface at the bottom of hole.Pitch-row panel edges 0.65cm, pitch of holes is 0.6cm.The thickness of sheet material is 0.5cm, hole depth 0.1cm.Shown in Figure 2.
Embodiment 3
Plate body adopts poly-third ethene material, and plate body length is 9.7cm, and width is 4.9cm, and thickness is 0.1cm; Plate body is made a call to 32 holes, and distribute in 8*4, hole is square, and the length of side is 0.8cm, is planar bottom surface at the bottom of hole.Pitch-row panel edges 0.25cm, pitch of holes is 0.4cm, hole depth 0.02cm.Shown in Figure 3.
Claims (7)
1. for a high flux check-out console for single cell gel electrophoresis test, it is characterized in that a plate face of described check-out console having multiple rule, mutual disconnected aperture,
Wherein said hole is square hole, and the length of side is 0.8-1.2cm; Or
Wherein said hole is circular port, and radius is 0.4cm.
2. high flux check-out console according to claim 1, is characterized in that, the plate body length of described plate is 3.5-12.5cm, and width is 3.5-8.5cm, and thickness is 0.1-0.5cm.
3. high flux check-out console according to claim 1, is characterized in that, the quantity in described hole is 4-32.
4. high flux check-out console according to claim 1, is characterized in that, described hole depth is 0.02 ~ 0.1cm.
5. high flux check-out console according to claim 1, is characterized in that, described pitch-row panel edges 0.25-0.65cm.
6. high flux check-out console according to claim 1, is characterized in that, described pitch of holes 0.4-0.8cm.
7. high flux check-out console according to claim 1, is characterized in that, described sheet material matter is selected from glass, poly-third ethene or polypropylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320138382.5U CN204666416U (en) | 2013-03-25 | 2013-03-25 | A kind of high flux check-out console for single cell gel electrophoresis test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320138382.5U CN204666416U (en) | 2013-03-25 | 2013-03-25 | A kind of high flux check-out console for single cell gel electrophoresis test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204666416U true CN204666416U (en) | 2015-09-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320138382.5U Expired - Lifetime CN204666416U (en) | 2013-03-25 | 2013-03-25 | A kind of high flux check-out console for single cell gel electrophoresis test |
Country Status (1)
| Country | Link |
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| CN (1) | CN204666416U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950467A (en) * | 2016-05-18 | 2016-09-21 | 清华大学 | Method for detecting cytotoxicity of to-be-tested medicine to target cell and cell chip specially used by method |
| WO2017214822A1 (en) * | 2016-06-13 | 2017-12-21 | 石庆学 | Sample adding plate for single cell gel electrophoresis |
| CN107860812A (en) * | 2017-09-19 | 2018-03-30 | 海南热带海洋学院 | A kind of kit polyacrylamide gel electrophoresis glue and the volley of rifle fire intersect high flux spotting methods |
-
2013
- 2013-03-25 CN CN201320138382.5U patent/CN204666416U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950467A (en) * | 2016-05-18 | 2016-09-21 | 清华大学 | Method for detecting cytotoxicity of to-be-tested medicine to target cell and cell chip specially used by method |
| CN105950467B (en) * | 2016-05-18 | 2018-08-28 | 清华大学 | A kind of method detecting drug to be measured to the cytotoxicity of aim cell and its special cell chip |
| WO2017214822A1 (en) * | 2016-06-13 | 2017-12-21 | 石庆学 | Sample adding plate for single cell gel electrophoresis |
| CN107860812A (en) * | 2017-09-19 | 2018-03-30 | 海南热带海洋学院 | A kind of kit polyacrylamide gel electrophoresis glue and the volley of rifle fire intersect high flux spotting methods |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20150923 |
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| CX01 | Expiry of patent term |