CN204594918U - Anti-interference sensing electrode - Google Patents
Anti-interference sensing electrode Download PDFInfo
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- CN204594918U CN204594918U CN201520240889.0U CN201520240889U CN204594918U CN 204594918 U CN204594918 U CN 204594918U CN 201520240889 U CN201520240889 U CN 201520240889U CN 204594918 U CN204594918 U CN 204594918U
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Abstract
The utility model relates to a kind of bio-sensing electrode immediately detected for medical treatment, in particular to the anti-interference sensing electrode of one, described anti-interference district is provided with a Ge Kongsu district and a blocking-up district, described Kong Su district is arranged at injection port and blocks between district, described blocking-up district is arranged between Kong Su district and reaction zone, described Kong Su district is provided with mutually stacking ascorbic acid enzyme layer and hydrophilic material layer, and described blocking-up district is a fluid channel of filling powder to be dissolved.Kong Su district of the present utility model velocity ratio injection port and detection zone slow, and blood stops flowing after entering blocking-up district, by the time after powder dissolution to be dissolved, blood sample flows to enter detection zone again, blood is 15 ~ 60 seconds from the time of arrival detection zone, anti-interference district, ascorbinase ascorbic acid in anti-interference district and blood is fully reacted, when making blood enter detection zone, ascorbic acid content is lower, and the interference of the materials such as uric acid in blood when detecting by ascorbic acid, accuracy of detection is high.
Description
Technical field:
The utility model relates to a kind of bio-sensing electrode immediately detected for medical treatment, particularly a kind of micro-fluidic test paper with antijamming capability and preparation method thereof, belongs to biosensor technique field.
Background technology:
Uric acid is the dead end product of purine metabolism, and purine metabolic disturbance, abnormal energy metabolism and kidney all can cause Plasma Uric Acid concentration raise (hyperuricemia) or reduce (Hypouricemia) to the acatharsia of uric acid.Think at present, testing uric acid is the best biochemical marker of gout caused by diagnosis purine metabolic disturbance.The principal feature of gout is hyperuricemia, causes gouty acute arthritis, tophaceous deposition, tophaceous chornic arthritis and the renal lesions such as joint deformity, uric acid kidney stones therefrom, very large to health hazard.The detection of blood uric acid, has the quantification directive function of science to the development and change of the patient with gout state of an illness over the course for the treatment of.The detection of blood uric acid effectively can point out the hazards of diabetes, hypertension and cardiovascular and cerebrovascular disease, reduces the incidence of diabetes, hypertension and cardiovascular and cerebrovascular and merges the appearance of complication; The dietetic life of all right patient directions in time, prevents the generation of potential disease; Also contribute to the early diagnosis of renal lesions in addition.
There is the instant Test paper detected for uric acid in the market, adopt electrochemical method to carry out quantitative measurment.But owing to containing ascorbic acid in blood, the oxidation-reduction potential of ascorbic acid and uric acid is close, therefore can disturb the detection of uric acid.
Application number is 201210407884.3, utility model name is called a kind of state's patented claim of dry chemical scrip of quantitative test uric acid, by reaching jamproof object at printed on electrodes or coating ascorbic acid oxidase, but the anti-jamming effectiveness of this method is limited.Because when electrode detection district is entered in blood siphon, only has the very short time (as described embodiments 10 seconds) detection time, only some ascorbic acid and ascorbic acid oxidase react, some ascorbic acid and uric acid simultaneously and dielectric react generation current, and still can produce undesired signal.
Utility model content:
In order to overcome above-mentioned defect, the utility model aim to provide a kind of when detecting some composition in blood efficient solution except the anti-interference sensing electrode of the interference of ascorbic acid.
In order to realize the purpose of this utility model, the technical solution adopted in the utility model is: anti-interference sensing electrode, comprise the anti-interference district be arranged between injection port and detection zone, it is characterized in that: described anti-interference district is provided with a Ge Kongsu district and a blocking-up district, described Kong Su district is arranged at injection port and blocks between district, described blocking-up district is arranged between Kong Su district and reaction zone, described Kong Su district is provided with mutually stacking ascorbic acid enzyme layer and hydrophilic material layer, described ascorbic acid enzyme layer is positioned at above hydrophilic material layer, described blocking-up district is a fluid channel of filling powder bed to be dissolved.
Described hydrophilic material layer is gelatin, bovine serum albumin(BSA) or Carboxymethyl Cellulose.
Described powder bed to be dissolved is trehalose micro mist or starch micro mist.
Anti-interference sensing electrode also comprises 2 pores, and wherein 1 pore is positioned at rear end, anti-interference district, and another 1 pore is positioned at rear, reaction zone.
Described anti-interference district is 1.5:1 ~ 10:1 with the cavity volume ratio of reaction zone, and the cavity volume of reaction zone is 1-10 microlitre.
Anti-interference sensing electrode described in the utility model and micro-fluidic test paper runner flow rate control method, can also be used for the sensing electrode building the blood testing indexs such as glucose, T-CHOL, triglyceride, ketoboidies, lactic acid, prothrombin time, it all can realize the object of utility model.
One section, Kong Su district of the present utility model velocity ratio injection port and detection zone slow, and blood stops flowing a period of time after entering blocking-up district, by the time after powder dissolution to be dissolved, blood sample flows to enter detection zone again, blood is 15 ~ 60 seconds from the time of arrival detection zone, anti-interference district, ascorbinase ascorbic acid in anti-interference district and blood is fully reacted, when making blood enter detection zone, ascorbic acid content is lower, the interference of the materials such as uric acid in blood when detecting not by ascorbic acid, accuracy of detection is high.
Accompanying drawing explanation
Fig. 1 is the structural drawing of anti-interference sensing electrode of the present utility model;
Fig. 2 is the structural drawing of substrate;
Fig. 3 is the structural drawing of middle interlayer;
Fig. 4 is the structural drawing of mucigel;
Fig. 5 is the structural drawing of upper cover;
Wherein 1 injection port, 2 anti-interference districts, 3 detection zones, 4 electrodes, 5 substrates, interlayer in 6,7 Kong Su districts, 8 block district, 9 mucigels, 10 upper covers, 11 pores, 12 reaction zones.
Embodiment
Below in conjunction with specific embodiment, the utility model is further described.
According to Fig. 1, a kind of sensing electrode with antijamming capability, comprises injection port 1, anti-interference district 2 and 3 three, detection zone part.Wherein detection zone comprises reaction zone, reaction zone 12 is for detecting the materials such as uric acid, glucose, T-CHOL, triglyceride, ketoboidies, lactic acid, blood sucks from injection port, flow into anti-interference district, after anti-interference district stays for some time, continue to flow into detection zone react, the signal on the electrode of detection zone reads detected value by the instrument connected.Some detecting electrodes 4 are contained in detection zone.
According to Fig. 2-5, sensing electrode test paper is made up of substrate 5 (i.e. dielectric base), middle interlayer 6, mucigel 9 and upper cover 10.At the upper screen printing work electrode of dielectric base (i.e. substrate) with to electrode, and both extraction wires.Middle interlayer is pasted in dielectric base, is formed and comprise working electrode and to the sample introduction zone of electrode and reaction zone.Middle interlayer is the insulation film with double faced adhesive tape, can be PVC insulation film, PET insulation film etc.Mucigel is between middle interlayer and upper cover, and upper cover is made up of anti-interference district.
Anti-interference district contains 7, one, a Ge Kongsu district and blocks district 8 and pore 11, wherein Kong Su district is provided with ascorbic acid enzyme layer and the hydrophilic material layer of stacked on top arrangement, arrangement mode is ascorbic acid enzyme layer-hydrophilic material layer, and blocking district is a fluid channel being filled with powder to be dissolved.One section, Kong Su district velocity ratio injection port and reaction zone slow.Blood is 15 ~ 60 seconds from the time of arrival detection zone, anti-interference district.Pore is contained in rear end in anti-interference district, liquid can be made to form siphon and be filled whole anti-interference district smoothly.There is another pore at rear, detection zone, and when after the powder dissolution to be dissolved blocking district, form second time siphon, liquid is from inflow detection zone, anti-interference district.
Powder to be dissolved is trehalose micro mist or starch micro mist.
Anti-interference district is (1.5 ~ 10) with the cavity volume ratio of reaction zone: 1.The cavity volume of reaction zone is 1-10 microlitre.Sample size 3-100 microliters of blood.
Preparation embodiment 1
Test paper is provided with injection port, detection zone and anti-interference district, and injection port is identical with the uric acid Test paper of prior art with detection zone.
Arrange Kong Su district in anti-interference district and block district, the cavity volume ratio in Kong Su district and blocking-up district is 3:1, and cavity volume is 10 microlitres and 3.3 microlitres, and corresponding reaction zone volume is 7 microlitres; Kong Su district arranges the ascorbic acid enzyme layer and hydrophilic material layer that are alternately arranged, and arrangement mode is ascorbic acid enzyme layer (thickness 0.1mm)-hydrophilic material layer (thickness 0.2mm).
Block the interior trehalose micro mist floor of filling 0.5mg of fluid channel in district.
Blood is 30 seconds from the time of arrival detection zone, anti-interference district.
Preparation embodiment 2
Test paper is provided with injection port, detection zone and anti-interference district, and injection port is identical with the uric acid Test paper of prior art with detection zone.
Arrange Kong Su district in anti-interference district and block district, the cavity volume ratio in Kong Su district and blocking-up district is 5:1, and cavity volume is 10 microlitres and 2 microlitres, and corresponding reaction zone volume is 8 microlitres; Kong Su district arranges the ascorbic acid enzyme layer and hydrophilic material layer that are alternately arranged, and arrangement mode is ascorbic acid enzyme layer (thickness 0.1mm)-hydrophilic material layer (thickness 0.2mm).
Block the interior starch micro mist floor of filling 0.5mg of fluid channel in district.
Blood is 45 seconds from the time of arrival detection zone, anti-interference district.
Preparation embodiment 3
Test paper is provided with injection port, detection zone and anti-interference district, and injection port is identical with the uric acid Test paper of prior art with detection zone.
Arrange Kong Su district in anti-interference district and block district, the cavity volume ratio in Kong Su district and blocking-up district is 6:1, and cavity volume is 12 microlitres and 2 microlitres, and corresponding reaction zone volume is 9 microlitres; Kong Su district arranges the ascorbic acid enzyme layer and hydrophilic material layer that are alternately arranged, and arrangement mode is ascorbic acid enzyme layer (thickness 0.1mm)-hydrophilic material layer (thickness 0.2mm).
Block the fluid channel interior filling 0.4mg trehalose micro mist floor in district.
Blood is 60 seconds from the time of arrival detection zone, anti-interference district.
Comparative example 1
To detect uric acid, enter blood flow volume and be set as 10 microlitres, blood sample is measured respectively when uric acid concentration is 0.3mmol/L with common uric acid electrode and anti-interference sensing electrode, ascorbic acid is respectively 0/0.02/0.05/0.075/0.1/0.2/0.3/0.5/0.75/1mmol/L, response current situation of change, ascorbic acid as shown in table 1 current-responsive Data Comparison table in uric acid test piece.
The current-responsive data of table 1 ascorbic acid in uric acid test piece
Interpretation of result: use anti-interference uric acid electrode and common uric acid electrode test ascorbic acid concentrations to be respectively the 0.3mmol/L uric acid solution of 0/0.02/0.05/0.075/0.1/0.2/0.3/0.5/0.75/1mmol/L, result shows, the current value of anti-interference uric acid electrode is stabilized in about 0.85 microampere, and the common electric current of uric acid electrode is directly proportional to the concentration of ascorbic acid, anti-interference uric acid electrode is described not by the impact of ascorbic acid concentrations.
Comparative example 2
To detect uric acid, enter blood flow volume and be set as 15 microlitres, measuring uric acid concentration respectively with anti-interference sensing electrode is 0.1/0.2/0.3/0.4/0.5/0.6/0.8/1.2mmol/L, ascorbic acid concentrations be 0 and 1mmol/L time, response current situation of change, anti-interference uric acid electrode detection uric acid Comparative result table as shown in table 2.
Table 2 anti-interference uric acid electrode detection uric acid Comparative result data
Interpretation of result: use anti-interference uric acid electrode test ascorbic acid concentrations to be respectively 0 and 1mmol/L, the uric acid concentration solution that is 0.1/0.2/0.3/0.4/0.5/0.6/0.8/1.2mmol/L, result shows, the result of two groups of tests is close, current value is directly proportional to the concentration of uric acid, current value containing ascorbic acid group does not increase, and anti-interference uric acid electrode is described not by the impact of ascorbic acid concentrations.
More than show and describe ultimate principle of the present utility model, principal character and advantage.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment and instructions just illustrates principle of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (6)
1. anti-interference sensing electrode, comprise the anti-interference district be arranged between injection port and detection zone, it is characterized in that: described anti-interference district is provided with a Ge Kongsu district and a blocking-up district, described Kong Su district is arranged at injection port and blocks between district, described blocking-up district is arranged between Kong Su district and reaction zone, described Kong Su district is provided with mutually stacking ascorbic acid enzyme layer and hydrophilic material layer, described ascorbic acid enzyme layer is positioned at above hydrophilic material layer, and described blocking-up district is a fluid channel of filling powder bed to be dissolved.
2. anti-interference sensing electrode according to claim 1, is characterized in that: described hydrophilic material layer is gelatin, bovine serum albumin(BSA) or Carboxymethyl Cellulose.
3. anti-interference sensing electrode according to claim 1 and 2, is characterized in that: described powder bed to be dissolved is trehalose micro mist or starch micro mist.
4. anti-interference sensing electrode according to claim 3, is characterized in that: also comprise 2 pores, and wherein 1 pore is positioned at rear end, anti-interference district, and another 1 pore is positioned at rear, reaction zone.
5. anti-interference sensing electrode according to claim 1, is characterized in that, described hydrophilic material layer is Carboxymethyl Cellulose.
6. anti-interference sensing electrode according to claim 5, is characterized in that, described anti-interference district is 1.5:1 ~ 10:1 with the cavity volume ratio of reaction zone, and the cavity volume of reaction zone is 1-10 microlitre.
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| CN201520240889.0U CN204594918U (en) | 2015-04-21 | 2015-04-21 | Anti-interference sensing electrode |
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| CN201520240889.0U CN204594918U (en) | 2015-04-21 | 2015-04-21 | Anti-interference sensing electrode |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104777194A (en) * | 2015-04-21 | 2015-07-15 | 苏州市玮琪生物科技有限公司 | Anti-interference sensing electrode and microfluidics test paper runner flow velocity control method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104777194A (en) * | 2015-04-21 | 2015-07-15 | 苏州市玮琪生物科技有限公司 | Anti-interference sensing electrode and microfluidics test paper runner flow velocity control method |
| CN104777194B (en) * | 2015-04-21 | 2017-10-27 | 苏州市玮琪生物科技有限公司 | Anti-interference sensing electrode and micro-fluidic test paper runner flow rate control method |
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