CN2045304U

CN2045304U - Bacterial-free separation tube for x/y spermatozoa

Info

Publication number: CN2045304U
Application number: CN 89203782
Authority: CN
Inventors: 王福楠
Original assignee: Individual
Current assignee: Individual
Priority date: 1989-04-05
Filing date: 1989-04-05
Publication date: 1989-10-04

Abstract

The utility model discloses a bacterial-free separation tube for x/y spermatozoa, comprising a glass tube and a rubber ball. The glass tube is in recumbent U character shape. A transparence supporting base is positioned at the starting part of the glass tube. The inner diameter of the glass tube tapers off at the beginning of the terminal of the starting part. The extreme end of the test tube is provided with a hollow rubber ball. The probability of the sex of the firstborn is more than 80% by using the separation tube to separate the X/Y spermatozoa.

Description

Bacterial-free separation tube for x/y spermatozoa

This utility model relates to spermatozoon and separates test tube, and the spermatozoon sample after particularly a kind of separable X/Y spermatozoon and the separation does not contain the test tube of antibacterial.

On modern medicine, can utilize methods such as ultrasonic scanning, amniocentesis to differentiate sex of fetus in advance, therefore, some family is when learning that fetus is not ideal sex, just adopt artificial abortion operation to have an abortion, this is quite popular in area, whole Asia, and near inhuman and the threat fetus rights to subsistence.And adverse effect also arranged to the anemia of pregnant woman is healthy.In order to address this problem, best bet is can control sex of foetus in advance, uses to help government to promote family planning.Improve the quality of the population, reach eugenic health.But up to now, on modern medicine, still do not have a kind of good pipe, can separate the X sperm y sperm in the seminal fluid.

Therefore, the test tube that the purpose of this utility model is to provide interior X sperm of a kind of separable seminal fluid and y sperm utilizes this to manage isolated sperm and carries out the artificial fertilization, further reaches the purpose of eugenic health.Certainly, this pipe except being used to separate human spermatozoon, also can be used for animal.

Use this utility model test tube, can not only isolate the X/Y spermatozoon, reach controlled other purpose, and the sperm after this pipe separates does not contain antibacterial, thereby prevents to accept women's uterine infection of artificial fertilization, can prevent to carry in the seminal fluid catching yet.

This utility model separates test tube and is made up of glass tubing and ball, glass tubing is to be horizontal type U-shaped pipe, in the tube chamber of the hypomere start-up portion of pipe, be provided with the semilune knuckle, start-up portion is with transparent bearing, test tube hypomere upper limb has an air trapping space, in vitro through from the terminal beginning of start-up portion and be tapered, become the U font after, horizontal-extending.The horizontal tail end of test tube epimere place can be provided with a best Y spermatozoon collecting region, establishes inverted-loop tube thereafter again, and the back of this pipe is vertically upward a pipe, is provided with a hollow rubber bulb at this vertical tube end.At the front end and the tail end of test tube horizontal segment, be carved with the indentation of frangibility.

Fig. 1 is the axonometric chart of this utility model separator tube;

Now separator tube structure of the present utility model is described in conjunction with Fig. 1.

This separator tube is made up of clear glass (or plastics) and ball, glass part is a U-shaped double glazing pipe that is horizontal type, terminal from the start-up portion of U-shaped glass tubing, to going up caliber from the coarse to fine down, end at the U-shaped glass tubing connects a reverse U shape glass tubing, at the end of this inverted-loop tube, vertically upward a pipe is arranged, terminal at this pipe, just the top of this separator tube is a hollow rubber bulb t.

As shown in Figure 1, the H of this separation pipe partly is the first quarter moon shape solid glass of a protuberance, protrudes in the tube chamber.G partly is a solid tetragonal glass shelf, in order to keep the stability of whole separator tube.The internal diameter of F part pipe is 6 millimeters, and promptly the M point is 6 millimeters to the N point.(Q point) is 6.1 millimeters to the A point from the air trapping space, about 2 centimeters away from pipe start-up portion opening part of Q points be, and after the A point, the internal diameter of pipe little by little attenuates and is bent upwards, and then forms horizontal U word shape, up to the D point.The internal diameter that D is ordered is 1 millimeter, be a best Y spermatozoon collecting region R after the D point, its capacity is 0.01 cc, is reverse U shape pipe connection one pipe vertically upward from the D point to the E point, on C point, D point and J point place tube wall annular indentation is arranged, the person fractures easily to be convenient to the clinical manipulation.Take out good spermatozoon sample in this section pipe, for clinical use.

The capacity of ordering from the P point to C is 0.15 cc, and the capacity of ordering from the C point to D is 0.12 cc, and the capacity of ordering from the D point to E is 0.06 cc, and the capacity of ordering from the A point to P is 0.05 cc.The spatial capacity of air trapping is 0.01 cc, and the capacity of special good spermatozoon collecting region is 0.01 cc.The volume of rubber bulb is 0.5 cc.

The length of ordering from the N point to B is 3 centimeters, the length of ordering from the M point to L is 3 centimeters, the length of ordering from the A point to B is 1 centimeter, the length of ordering from the Q point to L is 1 centimeter, the outer surface length of ordering from the B point to C is 2 centimeters (scope 1.5-2.0 centimetres), is 1 centimeter (scope 0.5-1.0 centimetre) from the K point to the most direct distance of L point, and the length of ordering from the C point to D is 6 centimeters, the length of ordering from the D point to E is 4 centimeters, and the length of ordering from the A point to D is 9 centimeters.

Be that an inverted-loop tube connects a pipe vertically upward from the D point to the E point, its purpose is to prevent by in the air or the pollution of clinical manipulation process extrinsic factor, if the clinical position person operates under aseptic condition, can not want last this section U-shaped pipe yet, and unrestrained rubber bulb be placed on one section of special good spermatozoon collecting region back upwards vertical tube above.

The Q point is the air trapping district, when semen sample being put in the semen sample district, because the anthropic factor of operation sometimes can produce bubble.Collect these bubbles and can prevent that these bubbles from upwards swimming, and antibacterial is taken to our needed C-D section.

The P point is original semen sample district.

The R point is best spermatozoon collecting region, can collect the Y spermatozoon that swims the fastest.

Below concise and to the point principle and the use that this utility model separator tube separates the X/Y spermatozoon described.

Normal seminal fluid, every cc of spermatozoon that should contain more than 60,000,000.In the seminal fluid, X spermatozoon and ovum fertilization combination are the girl baby, and Y spermatozoon and ovum fertilization combination are the boy baby.Because the Y spermatozoon is little than the X spermatozoon in the seminal fluid, but mobility is big than the X spermatozoon, therefore within a certain period of time, in same pipe, the Y spermatozoon longer distance of can swimming.Pipe is long more, and at the back segment of pipe, the Y spermatozoon of collecting is many more.If get about 1 centimeter long pipe, " spermatozoon bullet " (Sperm Pellet) after centrifugal, in containing pipe tissue culture medium, long 1 centimeter, freely swim, after one hour, get top half upper strata liquid and do the evaluation of X sperm, the ratio of Y spermatozoon and X spermatozoon is 1.35:1, and in normal 1 liquid, the ratio of Y spermatozoon and X spermatozoon is 1.06:1.Use separator tube of the present utility model, spermatozoon swims apart from reaching 9 centimeters, the spermatozoon that the pipe second half section collects, the ratio of its Y and X is 4-5:1, and this section sample accommodating little (0.12 cc), and is fit to do intrauterine artificial fertilization art (notes: intrauterine artificial fertilization art, injecting intrauterine liquid should not be more than 0.5 cc), that is to say, adopt this separator tube to come separated sperm, control is given birth to men and women's probability more than at least 80%.

It is operating as: get 1-2 cc of seminal fluid, the tissue culture medium that adds 3 cc, in centrifuge tube centrifugal after, go upper strata centrifugal liquid, the spermatozoon bullet of getting at the bottom of the centrifuge tube pipe places on the P point position of this separator tube, and separator tube need fill up earlier and be incubated 37 ℃ tissue culture medium.Above-mentioned separator tube is placed on 5% carbon dioxide, in 37 ℃, the couveuse of 95% humidity about 1-1.5 hour, the wave spermatozoon swims automatically, get the spermatozoon sample in the C-D section, if in the C-D section, spermatozoon is numerous, the latter half of desirable C-D section, do intrauterine artificial fertilization art, promptly contain the Y spermatozoon of high ratio.In addition, can not carry the principle of microorganism, in spermatozoon swims process, separate with antibacterial, impurity and microorganism based on spermatozoon.Again, spermatozoon is through turning left from the right side, more from bottom to top, swimming from left to right then, by way of two challenge angles, like this, swimming over to last spermatozoon must be the strongest high-quality spermatozoon of mobility.

Claims

1, a kind of aseptic X/Y spermatozoon separator tube, it is characterized in that forming by glass tubing and ball, glass tubing becomes horizontal U font, pipe hypomere start-up portion is provided with transparent rack, be provided with the semilune knuckle in the tube chamber of pipe hypomere start-up portion, pipe hypomere upper limb has an air trapping space, and bore from bottom to top is tapered and bends to horizontal-extending behind the U type from start-up portion, connect vertically upward a pipe then, a hollow rubber bulb is arranged on this pipe top.

2, separator tube as claimed in claim 1 is characterized in that the tail end of pipe epimere horizontal-extending pipe being provided with a best spermatozoon collecting pit.

3, separator tube as claimed in claim 1 or 2 is characterized in that establishing a U type pipe at horizontal-extending Guan Shangzai.

4, separator tube as claimed in claim 1 or 2 is characterized in that having the indentation of frangibility on the horizontal front end of pipe and tail end tube wall.

5, separator tube as claimed in claim 3 is characterized in that having the indentation of frangibility on the horizontal segment front end of pipe and tail end tube wall.