CN206512182U - A kind of sperm swim-up support - Google Patents
A kind of sperm swim-up support Download PDFInfo
- Publication number
- CN206512182U CN206512182U CN201720087273.3U CN201720087273U CN206512182U CN 206512182 U CN206512182 U CN 206512182U CN 201720087273 U CN201720087273 U CN 201720087273U CN 206512182 U CN206512182 U CN 206512182U
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- China
- Prior art keywords
- sperm
- test tube
- fixing groove
- support
- swim
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of sperm swim-up support.The sperm swim-up support includes the rectangular phosphor bodies being made up of light transmissive material, the main body is provided with an inclined-plane horizontal by 45° angle, the body lumen is provided with the column fixing groove of the one end open horizontal by 45° angle, and the openend of the fixing groove is located on inclined-plane.The utility model is simple in construction, it is easy to use, it is with low cost, test tube can either be maintained at optimal position during sperm swim-up, sample of sperm can be carried out in upper reaches method operating process directly observing and operating simultaneously again, prepare the efficiency of sperm so as to effectively improve upper reaches method and reduce operation difficulty.
Description
Technical field
The utility model belongs to Bioexperiment apparatus field, and in particular to one kind can either will be tried during sperm swim-up
Pipe（Such as round bottom common test tube and conical bottom centrifuge tube）Optimal position is maintained at, and simultaneously can be in upper reaches method operating process
In to sample of sperm carry out directly observe and operate device.
Background technology
According to the World Health Organization（WHO）The 10-15% couple at child-bearing age is perplexed by infertility in estimation, developed country.In development
In country（Including China）, the women of child-bearing age for having 1.86 hundred million to 2002 suffer from infertility.The statistics of 2014 shows,
The incidence of China's reproductive population infertility is 12.5%, and infertility number is more than 40,000,000.Vitro fertilization-embryo implanting（in-
vitro fertilization and embryo transfer, IVF-ET）Technology is the important hand of current treatment infertility
Section.From 1978 since the first " test-tube baby " in the world is born in Britain, current developed country helps the baby of pregnant birth by ART
Youngster has accounted for the 1-3% of natus sum, estimates the births of whole world test-tube baby more than 5,000,000 people.
Sperm in semen can not directly make oocyte fertilization in vitro, in vitro fertilization-embryo transfer
, it is necessary to be prepared in vitro to human sperm during technology is implemented, purpose prepared by sperm includes：The external of sperm is obtained
Can and sperm it is preferred.Only by preparing and reaching that it is in vitro fertilization that the human sperm of certain standard can be carried out.Upstream
Method is a kind of the most frequently used sperm preparation method, according to《The World Health Organization-human seminal fluid checks and treatment of laboratory handbook
(the 5th edition)》Recommendation method, progress upper reaches method, which prepares sperm, includes 3 key steps：1）Placed in test tube bottom after 1ml liquefaction
Fresh semen；2）Then 1.2ml sperm preparation mediums are up added, test tube is tilted into 45 ° is incubated 1 hour at 37 DEG C
Afterwards, most great-hearted sperm by from the seminal fluid upstream of bottom into the culture medium on upper strata；3）Draw upper strata 1ml culture mediums and from
After heart concentration, you can obtain for sperm in vitro fertilization.In the operating process of upper reaches method, there are several technical essentials：First,
In step 1, the boundary between seminal fluid and culture medium is clear, is mutually immiscible；Secondly, in step 2, in order to obtain maximum
Organic efficiency, test tube should tilt 45 °, can so obtain the interfacial surface area between the seminal fluid and culture medium of maximum；Finally, walk
In rapid 3, when drawing upper strata culture medium, the refining of lower floor must not be mixed with, otherwise the refining of lower floor can suppress the capacitation of sperm.Cause
This, during upper reaches method prepares sperm, how strict guarantee these three technical essentials, be to obtain optimal efficiency in vitro fertilization
Key.
Because the process of sperm swim-up needs to be incubated 1 hour at 37 DEG C, therefore upper reaches method needs to use fixed support complete
Into.But in current laboratory in vitro fertilization, the special sperm swim-up support of standard is there is no at present.What is used at present replaces
Mainly include for product：Test tube, i.e., be placed on the metal derby of fixing hole or foam block by metallic support and foam stand.But this
There is certain defect in a little supports：First, these supports are lighttight, and culture medium is added and draw in upper reaches method step 1 and 3
When, for the interface of seminal fluid visible in detail and culture medium, it is required for taking out test tube from support, is now all easier to shake
It is dynamic to cause obscuring for interface so that the sperm that the refining and activity ratio of lower floor are poor enters in the culture medium on upper strata, notable shadow
Ring upper reaches method and reclaim capacitation and the quality of sperm, and then have a strong impact on efficiency in vitro fertilization, even result in mistake in vitro fertilization
Lose；Secondly, the fixing hole on metal derby or foam block that most of IVF laboratories are used is vertical direction, in sperm swim-up
When be difficult to obtain optimal sperm organic efficiency.Because there is drawbacks described above in traditional sperm swim-up support, it will in upper reaches method
Implementation process in more depend on operator experience and operative skill, this is not only unfavorable for the standardization of technology, Er Qiecun
In potential operation failure risk.
Therefore, a kind of sperm swim-up support of standardization how is made, semen sample can either directly be observed
And operation, it is to avoid seminal fluid is obscured with culture medium interface, but simultaneously can by test tube it is reliable and stable be fixed on 45° angle to obtain
Optimum recovery rate, is that most laboratories in vitro fertilization are still needed the problem of solution.
Utility model content
The utility model is intended to overcome the deficiencies in the prior art there is provided a kind of sperm swim-up support, and the support can either be by
Test tube it is reliable and stable 45° angle is fixed on to obtain optimum recovery rate, and single semen sample can be carried out simultaneously direct
Observation and operation, it is to avoid seminal fluid is obscured with culture medium interface.
To achieve these goals, the technical scheme that the utility model is used is：
The sperm swim-up support includes the rectangular phosphor bodies being made up of light transmissive material, and the main body is provided with one and level
Face is in the inclined-plane of 45° angle, and the body lumen is provided with the column fixing groove of the one end open horizontal by 45° angle, described solid
The openend for determining groove is located on inclined-plane.
Wherein, the fixing groove internal diameter is 1-1.2 times of test tube diameter, and the depth of the fixing groove is effectively long for test tube
0.4-0.6 times of degree.The test tube is round bottom common test tube or conical bottom centrifuge tube.
The utility model is described in further detail below：
Sperm swim-up support described in the utility model is made angle in the similar cuboid block formed, main body by light transmissive material
There is a fixed channel opening on 45° angle inclined-plane, inclined-plane, and oliquely downward form the cylindrical fixing groove of a 45° angle, fixing groove
The end is blind hole, and bottom is circular arc or cone, is agreed with test tube bottom.
Thus, test tube is placed in fixing groove, can either by test tube it is reliable and stable be fixed on 45° angle, it is again convenient to see
Examine and operate, when adding and drawing upper strata culture medium without mobile test tube.
The internal diameter of the fixing groove is 1-1.2 times of test tube diameter, and the depth of the fixing groove is test tube effective length
0.4-0.6 times.Because the excessive fixed effect that may result in of fixing groove internal diameter declines, too small, test tube can not be put into；And fixing groove
Length is oversize, is inconvenient to pick and place test tube, too short, test tube easily falls.The length on the inclined-plane and width are fixing groove internal diameters
1.5-2 times, otherwise it can not be open.
Preferably, the light-passing board material lucite, plastics or resin block.
Preferably, only one of which fixing groove on each support, so each support can only place a sperm model, maximum
Degree avoids the interfering when cross pollution and operation of sample room.
Preferably, the test tube is round bottom common test tube or conical bottom centrifuge tube.
Preferably, a length of 40-80mm of main body that the light transmissive material makes, width is 30-50mm, a height of 40-80mm；Institute
The length for stating inclined-plane is 30-50mm, and width is 30-50mm；The a length of 30-60mm of fixing groove, internal diameter is 10-20mm.It is preferred that
Concrete numerical value is formulated according to these three the most frequently used test tubes.Common 5ml round bottom common test tubes a diameter of 12mm, a length of 75mm；
14ml round bottom common test tubes a diameter of 17mm, a length of 100mm；15ml conical bottom centrifuge tubes a diameter of 17mm, a length of 120mm.
Main body uses the light transmissive material of high rigidity high transmission rate in the utility model（Such as lucite, plastics or resin
Deng）, it is easy to observe test tube and operated.All edges prevent from hurting operator's skin using polishing Passivation Treatment
Skin.In use, the support is placed in operating table surface, fixing groove opening upwards, then test tube is inserted in fixing groove, sideways direction
Operator.Now, test tube is by reliable and stable fixation and upward 45° angle position；Operator can be by directly to test tube simultaneously
Directly observe and operate.So upper strata culture medium is added in upper reaches method step 1 and step 3 draws upper strata culture medium
When, the position of test tube will not be moved, it will be difficult to occur operational error, also can in addition in step 2 incubator during sperm swim-up
Obtain optimal organic efficiency.The utility model can be in operating desk and incubator the test tube by sperm swim-up（Such as round bottom
Common test tube or conical bottom centrifuge tube）Be placed in best angle, at the same can in the case of support observing to test tube
And operation.Overcome conventional stent heating plate upstream inefficiency, and can not carry out carrying out test tube what is directly observed and operate
Shortcoming, and it is simple in construction, easy to use, with low cost, it can effectively improve upper reaches method and prepare the efficiency of sperm and reduce behaviour
Make difficulty, at utmost obtain the sperm of high-quality in vitro fertilization.
Compared with prior art, the beneficial effects of the utility model are：
The utility model can in upper reaches method operating process, can by test tube it is reliable and stable be fixed on 45° angle, it is ensured that
Optimal sperm organic efficiency is obtained, while the semen sample in test tube and the education of upper strata culture medium are directly observed and operated,
The risk for picking and placeing cuvette belt repeatedly is avoided, the shortcoming of traditional sperm upstream support is overcome.
The utility model is simple in construction, easy to use, with low cost, can effectively improve the effect that upper reaches method prepares sperm
Rate simultaneously reduces operation difficulty, at utmost obtains the sperm of high-quality in vitro fertilization.
The utility model is except suitable for Human in vitro Fertilization laboratory, being also applied for other needs to test tube（Such as round bottom
Common test tube or conical bottom centrifuge tube）Tilt zoology, embryology, cell biology and micro- life that certain angle is operated
The laboratories such as thing.
Brief description of the drawings
Fig. 1 is the utility model structural representation；
Fig. 2 is profiles of the Fig. 1 according to A-A directions.
In figure：1st, inclined-plane；2nd, fixing groove；3rd, main body.
Embodiment
Referring to Fig. 1-2, the sperm swim-up support includes the rectangular phosphor bodies 3 being made up of light transmissive material, the main body 3
An inclined-plane 1 horizontal by 45° angle is provided with, the inner chamber of main body 3 is provided with the one end open horizontal by 45° angle
Column fixing groove 2, the openend of the fixing groove 2 is located on inclined-plane 1.
Wherein, the internal diameter of fixing groove 2 is 1-1.2 times of test tube diameter, and the depth of the fixing groove 2 is effective for test tube
0.4-0.6 times of length.The test tube is round bottom common test tube or conical bottom centrifuge tube.
Wherein, the light-passing board material is lucite, plastics or resin, preferably lucite.The light transmissive material
The a length of 40-80mm of main body 3 of making, width is 30-50mm, a height of 40-80mm；The length on the inclined-plane 1 is 30-50mm, wide
Spend for 30-50mm；The a length of 30-60mm of the fixing groove 2, internal diameter is 10-20mm.
Claims (3)
1. a kind of sperm swim-up support, it is characterised in that the sperm swim-up support includes the cuboid being made up of light transmissive material
Main body（3）, the main body（3）Provided with an inclined-plane horizontal by 45° angle（1）, the main body（3）Inner chamber is provided with and water
Plane is in the column fixing groove of the one end open of 45° angle（2）, the fixing groove（2）Openend be located at inclined-plane（1）On.
2. sperm swim-up support as claimed in claim 1, it is characterised in that the fixing groove（2）Internal diameter is test tube diameter
1-1.2 times, the fixing groove（2）Depth be 0.4-0.6 times of test tube effective length.
3. sperm swim-up support as claimed in claim 2, it is characterised in that the test tube is round bottom common test tube or conical bottom
Centrifuge tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720087273.3U CN206512182U (en) | 2017-01-23 | 2017-01-23 | A kind of sperm swim-up support |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720087273.3U CN206512182U (en) | 2017-01-23 | 2017-01-23 | A kind of sperm swim-up support |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206512182U true CN206512182U (en) | 2017-09-22 |
Family
ID=59867428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720087273.3U Expired - Fee Related CN206512182U (en) | 2017-01-23 | 2017-01-23 | A kind of sperm swim-up support |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206512182U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110548304A (en) * | 2019-09-07 | 2019-12-10 | 天津药明康德新药开发有限公司 | centrifugal concentrator for removing boiling point solvent |
-
2017
- 2017-01-23 CN CN201720087273.3U patent/CN206512182U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110548304A (en) * | 2019-09-07 | 2019-12-10 | 天津药明康德新药开发有限公司 | centrifugal concentrator for removing boiling point solvent |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170922 Termination date: 20200123 |