CN204514934U - A kind of Quantitative detection Soluble growth stimulates the test paper of expressing gene 2 albumen - Google Patents
A kind of Quantitative detection Soluble growth stimulates the test paper of expressing gene 2 albumen Download PDFInfo
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- CN204514934U CN204514934U CN201520186562.XU CN201520186562U CN204514934U CN 204514934 U CN204514934 U CN 204514934U CN 201520186562 U CN201520186562 U CN 201520186562U CN 204514934 U CN204514934 U CN 204514934U
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Abstract
The utility model discloses the test paper that a kind of Quantitative detection Soluble growth stimulates expressing gene 2 albumen, described test paper comprises support pad (1), be positioned at the nitrocellulose filter (2) in described support pad, one end of described nitrocellulose filter connects adsorptive pads (3), the other end connects conjugates pad (4), described conjugates pad connects sample pad (5), the below of described sample pad the other end is provided with the first fixed part (6), top is provided with the second fixed part (7), it is characterized in that, described nitrocellulose filter (2) middle part detection line (8) is coated with Soluble growth stimulates expressing gene 2 protein antibodies, detection line both sides are respectively equipped with control line (9), the Soluble growth described conjugates pad (4) being coated with gold mark stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates.
Description
Technical field
The utility model belongs to clinical diagnose field, is specifically related to a kind of test paper stimulating expressing gene 2 albumen for quantitatively detecting Soluble growth.
Background technology
Soluble growth stimulates expressing gene 2 albumen (ST2) gene to be first obtained in BALB/c-3T3 clone by Tominaga etc. for 1989, one of I L-1 (interleukin 1) receptor family member, this gene expression two kinds of protein products: a kind of with transmembrane structure, be called cross-module type ST2 (ST2L), one can be secreted into extracellular, is called secreting type ST2 (sST2).Research finds that ST2 can be expressed by cardiac fibroblast and cardiac muscle cell, is a kind of myocardium protein that Biomechanical force induction produces.ST2 gene expression is in Th2 cells (Th2) macrophage of mast cells activation and cardiac muscle cell.The ST2 gene of people is about 40kb, is positioned at human chromosomal 2q12, and a kind of soluble protein (sST2) of codified and a kind of cross-film form albumen (ST2L), both transcribing is subject to different promoter regulations respectively.
Research shows that sST2 is the Decoy receptor of IL-33; it can be combined with IL-33; thus block IL-33 and ST2L combination; then the cardiovascular protective effect weakening IL-33/ST2L signal path is subject to excessive tractive at cardiac muscle and causes in the process of damage; a large amount of sST2 generates the protection making cardiac muscle lack enough IL-33; thus accelerate myocardial remodelling and ventricular dysfunction, finally cause mortality risk to increase.IL-33 is IL-1 family member, can the membrane receptor on target cell be combined after mediate downstream signal path, or the nucleus being transported to target cell is as DNA binding factor functionating.Research shows, mechanical stress can produce IL-33 by cardiac stimulus fibroblast, after combining with its receptor complex (being made up of ST2L and IL-1RAcP), activation signals is passed in cell, a series of signal molecules such as the IL-1 related protein kinase marrow sample differentiation factor 88 through downstream and tumor necrosis factor receptor-associated factor, activate expression of nuclear factor kappa B (nuclear factor κ B, NF-κ B) and mitogen-activated protein kinase (mitogen-activated protein kinase, MAPK), thus regulatory gene is transcribed, cause the release of Th2 effector molecule IL-5 IL-4 and IL-13 etc.When cardiac fibroblast and cardiac muscle cell are subject to excessive pressure load, these effector molecules can make heart make adaptation reaction, and the myocardial hypertrophy preventing excessive tractive from causing and myocardial fibrosis occur.Meanwhile; cardiac muscle sST2 generates in a large number, and sST2 and IL-33 combines rear Reverse transcriptase, and it is combined with ST2L, blocks the intracellular signaling through ST2L; final suppression NF-B and MAPK activates, thus greatly reduces the endogenous myocardial effect of IL-33/ST2L signal path.ST2 can independently diagnosis of heart failure, during in conjunction with NT-proBNP, its to the Sensitivity and Specificity of the diagnosis of heart failure all higher than the result that ST2 and NT-proBNP diagnoses separately.Can be good at the mortality ratio of prediction sufferer in 1 year based on ST2 level, so it provides Data support when instructing sufferer whether to carry out heart transplant, being conducive to cure rate and the survival rate of intervening raising sufferer in advance.
At present, detecting Soluble growth stimulates the current mainly Enzyme-multiplied immune technique (ELISA) of the method for expressing gene 2 albumen, and elisa technique exists following shortcoming: checkout equipment requires high, and cost is high; Disturbing factor is more, and repeatability is bad; Detection time is long.Therefore Enzyme-multiplied immune technique detects Soluble growth stimulates expressing gene 2 albumen to be not suitable for clinical quick diagnosis.In view of there is no quantitative detecting method fast at present, the purpose of this utility model is to provide a kind of Quantitative detection Soluble growth that can be used for stimulates the test paper of expressing gene 2 albumen and a kind of paper box that can be used for Quantitative detection Soluble growth stimulation expressing gene 2 albumen, and described test paper and paper box have the advantages such as convenient and swift, simple to operate, result is accurate.
Utility model content
An object of the present utility model is to provide the test paper that a kind of Quantitative detection Soluble growth stimulates expressing gene 2 albumen, Quantitative detection Soluble growth described in the utility model stimulates the test paper of expressing gene 2 albumen to have the advantages such as convenient and swift, simple to operate, result is accurate, is suitable for clinical quick diagnosis.
The technical solution of the utility model is:
A kind of test paper stimulating expressing gene 2 albumen for quantitatively detecting Soluble growth, described test paper comprises support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter, stacked connection conjugates pad 4 above the other end, stacked connection sample pad 5 above described conjugates pad, the below of described sample pad the other end is provided with the first fixed part 6, top is provided with the second fixed part 7, it is characterized in that, described nitrocellulose filter 2 middle part is provided with detection line 8 and is coated with Soluble growth stimulates expressing gene 2 protein antibodies, detection line both sides are respectively equipped with control line 9, the Soluble growth described conjugates pad 4 being coated with gold mark stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates.
Further, described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.
Further, described first fixed part 6 is double faced adhesive tape, and the second fixed part 7 is one side glue.
Further, described test paper also comprises a shell 10 outward, and described shell is wrapped in outside described test paper, exposes detection line 8 on sample pad 5, nitrocellulose filter 2 and two control lines 9 and adsorptive pads 3.
The preferred plastic casing of described shell.
Further, described two control lines 9 and detection line 8 are for be arrangeding in parallel.
Paper box described in the utility model has the advantage being convenient for carrying and storing.
The test paper of Quantitative detection Soluble growth stimulation expressing gene 2 albumen described in the utility model or the principle of work of paper box are: adopt two-way effluent immune response principle, be prepared from by double antibody sandwich method.Soluble growth during inspection in sample stimulates expressing gene 2 proteantigen first to stimulate expressing gene 2 protein antibodies conjugates generation immune response with the Soluble growth on coupling pad, forms immune complex.Thereafter immune complex is along with sample chromatographic flow on NC Nitroncellulose film, when immune complex chromatography is to detection zone (ST2 detection line) on NC Nitroncellulose film, expressing gene 2 protein antibodies is stimulated to react thus be fixed on the detection line of NC Nitroncellulose film with the anti-Soluble growth be coated in advance on NC Nitroncellulose film.Soluble growth in blood stimulates expressing gene 2 albumen more, and the compound on detection line is more, and the optical density value on band is higher.Simultaneously, in testing process, control line conjugates (i.e. the collaurum of DNP-BSA mark), also can with sample chromatography on NC Nitroncellulose film, when chromatography is to the control line of NC Nitroncellulose film, DNP-BSA collaurum can react with the anti-DNP antibody be coated in advance on NC Nitroncellulose film thus be fixed on check plot (control line).Because collaurum is red, the DNP-BSA colloid gold immune compound be thus fixed in the anti-DNP on NC Nitroncellulose film control line will show redness.When not containing Soluble growth in sample and stimulating expressing gene 2 albumen, then only show two control lines.When sample stimulates expressing gene 2 albumen containing Soluble growth, detection line will show clearly.
After reaction terminates, multifunction immunity detector (auspicious Lay bioengineering (Shenzhen) company limited) is utilized the optical density of control line and detection line to be analyzed, and by analyze the result obtained and carry out computing, thus obtain relative optical density number (RI).Then detector stimulates the concentration of expressing gene 2 albumen calculate and show result according to the typical curve be set in advance in detector to Soluble growth, represents in units of ng/mL.
Accompanying drawing explanation
Fig. 1 is the structural representation that Quantitative detection Soluble growth described in the utility model stimulates a kind of preferred implementation of the test paper of expressing gene 2 albumen.
Wherein, 1: support pad, 2: nitrocellulose filter, 3: adsorptive pads, 4: conjugates pad, 5: sample pad, 6: the first fixed parts, 7: the second fixed parts, 8: detection line, 9: control line.
Fig. 2 is the diagram that Quantitative detection Soluble growth described in the utility model stimulates a kind of preferred implementation of the paper box of expressing gene 2 albumen.
Wherein, 3: adsorptive pads, 5: sample pad, 8: detection line, 9: control line, 10: shell.
Embodiment
Stimulate the test paper of expressing gene 2 albumen or paper box to be described in detail below in conjunction with accompanying drawing to Quantitative detection Soluble growth described in the utility model, can not be interpreted as it is to restriction of the present utility model.The material used in following examples and reagent unless otherwise indicated, are common commercially available.
[embodiment 1] prepares the test paper that Quantitative detection Soluble growth stimulates expressing gene 2 albumen
Test paper structure as shown in Figure 1, comprise support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter, stacked connection conjugates pad 4 above the other end, stacked connection sample pad 5 above described conjugates pad one end away from nitrocellulose filter 2, bonding first fixed part 6 in below of described sample pad the other end, preferred double faced adhesive tape, bonding second fixed part 7 in top, preferred one side glue, it is characterized in that, described nitrocellulose filter 2 middle part detection line 8 is coated with Soluble growth stimulates expressing gene 2 protein antibodies, detection line both sides are respectively equipped with control line 9, the Soluble growth described conjugates pad 4 being coated with gold mark stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates.
Described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.
Wherein, nitrocellulose filter 2 for fixing coated antibody, is also immunoreactive nidus in test paper simultaneously; Detection line 8 is stimulated by Soluble growth expressing gene 2 protein antibodies to use the damping fluid such as 1*PBS, methyl alcohol to be diluted to the concentration of 1mg/ml, gets 1ul/cm and line on described nitrocellulose filter 2, be drying to obtain; Control line 9 uses the damping fluid such as 1*PBS, methyl alcohol to be diluted to the concentration of 0.5mg/ml anti-DNP antibody, gets 1ul/cm and line on described nitrocellulose filter 2, be drying to obtain.
Wherein, the starting material of described conjugates pad 4 are glass fiber filter, glass fiber filter for the preparation of conjugates pad is put into pre-Block buffer and (comprises the casein of 0.1-1%, the PEG of the bovine serum albumin(BSA) of 0.5-5%, the boric acid of 0.005-0.5%, 0.01-0.2%) middle immersion taking-up after 5 minutes, after drying, the Soluble growth marked by gold with Biodot coating instrument stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates to be coated on conjugates pad 4, coating weight is 1ul/mm, drying, to obtain final product.Preferably, the pre-Block buffer of described conjugates pad 4 comprises the casein of 0.8%, the bovine serum albumin(BSA) of 4%, the boric acid of 0.007%, the PEG of 0.1%.
Wherein, described sample pad 5 can play preliminary filtering function by liquid towards sample.After sample pad is soaked 5 minutes with 141 confining liquids (comprising: the casein of the Tween20 of the Tris of 0.1-1%, 0.1-1%, 0.1-1%, the mouse anti-human RBC of 0.05-0.5%), 37 DEG C of dryings of spending the night, to obtain final product.Preferably, described 141 confining liquids comprise: the Tris of 0.3%, the casein of 0.8%, the mouse anti-human RBC of 0.3%.
Above-mentioned each building block is pasted onto in support pad 1 by structure shown in Fig. 1, obtains the test paper that Quantitative detection Soluble growth stimulates expressing gene 2 albumen.
[embodiment 2] stimulates expressing gene 2 albumen with test paper Quantitative detection Soluble growth described in the utility model
Get the test paper that the preferred a kind of Quantitative detection Soluble growth of the utility model stimulates expressing gene 2 albumen, as shown in Figure 1, described test paper comprises support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, stacked connection adsorptive pads 3 above one end of described nitrocellulose filter, stacked connection conjugates pad 4 above the other end, stacked connection sample pad 5 above described conjugates pad one end away from nitrocellulose filter 2, bonding first fixed part 6 in below of described sample pad the other end, preferred double faced adhesive tape, bonding second fixed part 7 in top, preferred one side glue, it is characterized in that, described nitrocellulose filter 2 middle part detection line 8 is coated with Soluble growth stimulates expressing gene 2 protein antibodies, detection line both sides are respectively equipped with control line 9, the Soluble growth described conjugates pad 4 being coated with gold mark stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates.Preferably, described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.Detect according to the following steps: the patients serum 1) extracted, blood plasma or whole blood wait for that sample originally, as Cord blood sample need return to room temperature.2) testing sample is added on sample pad 5, reaction.3) interpretation, is placed in multifunction immunity detector by described test paper, result of determination in SSJ-2 or MINI (auspicious Lay bioengineering (Shenzhen) company limited).
[embodiment 3] stimulates expressing gene 2 albumen with paper box Quantitative detection Soluble growth described in the utility model
Get the paper box that the preferred a kind of Quantitative detection Soluble growth of the utility model stimulates expressing gene 2 albumen, as depicted in figs. 1 and 2, described test paper comprises support pad 1, be positioned at the nitrocellulose filter 2 in described support pad, one end of described nitrocellulose filter connects adsorptive pads 3, the other end connects conjugates pad 4, described conjugates pad connects sample pad 5, bonding first fixed part 6 in below of described sample pad the other end, preferred double faced adhesive tape, bonding second fixed part 7 in top, preferred one side glue, it is characterized in that, described nitrocellulose filter 2 middle part detection line 8 is coated with Soluble growth stimulates expressing gene 2 protein antibodies, detection line both sides are respectively equipped with control line 9, the Soluble growth described conjugates pad 4 being coated with gold mark stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates.Preferably, described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.Described test paper also comprises a shell 10, and described shell is wrapped in described test paper, exposes detection line 8 on sample pad 5, nitrocellulose filter 2 and two control lines 9 and adsorptive pads 3.Detect according to the following steps: the patients serum 1) extracted, blood plasma or whole blood wait for that sample originally, as Cord blood sample need return to room temperature.2) testing sample is added on sample pad 5, reaction.3) interpretation, is placed in multifunction immunity detector by described test paper, result of determination in SSJ-2 or MINI (auspicious Lay bioengineering (Shenzhen) company limited).
Claims (5)
1. one kind stimulates the test paper of expressing gene 2 albumen for quantitatively detecting Soluble growth, described test paper comprises support pad (1), be positioned at the nitrocellulose filter (2) in described support pad, stacked connection adsorptive pads (3) above one end of described nitrocellulose filter, stacked connection conjugates pad (4) above the other end, stacked connection sample pad (5) above described conjugates pad, the below of described sample pad the other end is provided with the first fixed part (6), top is provided with the second fixed part (7), it is characterized in that, described nitrocellulose filter (2) middle part is provided with detection line (8) and is coated with Soluble growth stimulates expressing gene 2 protein antibodies, detection line both sides are respectively equipped with control line (9), the Soluble growth described conjugates pad (4) being coated with gold mark stimulates expressing gene 2 protein monoclonal antibody or polyclonal antibody and control line conjugates.
2. test paper as claimed in claim 1, it is characterized in that, described control line (9) is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.
3. test paper as claimed in claim 1, it is characterized in that, described first fixed part (6) is double faced adhesive tape, and the second fixed part (7) is one side glue.
4. the test paper according to any one of claim 1-3, it is characterized in that, described test paper also comprises a shell (10) outward, described shell is wrapped in outside described test paper, exposes detection line (8) on sample pad (5), nitrocellulose filter (2) and two control lines (9) and adsorptive pads (3).
5. test paper as claimed in claim 4, is characterized in that, described two control lines (9) and detection line (8) are for be arrangeding in parallel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520186562.XU CN204514934U (en) | 2015-03-30 | 2015-03-30 | A kind of Quantitative detection Soluble growth stimulates the test paper of expressing gene 2 albumen |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259353A (en) * | 2015-10-15 | 2016-01-20 | 北京市心肺血管疾病研究所 | Kit and method for detecting sST2 (soluble ST2) in blood of abdominal aortic aneurysm and/or aortic dissection patient |
| CN105445450A (en) * | 2015-12-30 | 2016-03-30 | 天津诺星生物医药科技有限公司 | Congestive heart failure detection system |
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2015
- 2015-03-30 CN CN201520186562.XU patent/CN204514934U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105259353A (en) * | 2015-10-15 | 2016-01-20 | 北京市心肺血管疾病研究所 | Kit and method for detecting sST2 (soluble ST2) in blood of abdominal aortic aneurysm and/or aortic dissection patient |
| CN105259353B (en) * | 2015-10-15 | 2017-03-22 | 北京市心肺血管疾病研究所 | Kit and method for detecting sST2 (soluble ST2) in blood of abdominal aortic aneurysm and/or aortic dissection patient |
| CN105445450A (en) * | 2015-12-30 | 2016-03-30 | 天津诺星生物医药科技有限公司 | Congestive heart failure detection system |
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Address after: 518000 Nanshan Medical Device Industrial Park BF02-BF04, No. 1019, Nanhai Avenue, Yanshan Community, Merchants Street, Nanshan District, Shenzhen, Guangdong Province Patentee after: ruilai bioengineering Co.,Ltd. Address before: 518000 B501 Nanshan Medical Equipment Industrial Park, Nanshan Nanhai Avenue, Shenzhen, Guangdong Province Patentee before: RELIA BIOLOGICAL ENGINEERING (SHENZHEN) CO.,LTD. |