CN204462089U - A kind of carbon nano-fiber mixed type integral post pretreatment unit - Google Patents
A kind of carbon nano-fiber mixed type integral post pretreatment unit Download PDFInfo
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- CN204462089U CN204462089U CN201520153487.7U CN201520153487U CN204462089U CN 204462089 U CN204462089 U CN 204462089U CN 201520153487 U CN201520153487 U CN 201520153487U CN 204462089 U CN204462089 U CN 204462089U
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- fiber
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- mixed type
- pretreatment unit
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Abstract
The utility model discloses a kind of carbon nano-fiber mixed type integral post pretreatment unit, it is filled with the carbon nano-fiber separating column that two kinds of heterogeneity carbon nano-fibers modify mixed type post in comprising, two ends are connected to sample feeding device and UV-detector with carbon nano-fiber separating column, the other end of sample feeding device is provided with delivery eluting solvent transmitting device, delivery eluting solvent transmitting device is provided with eluting solvent storage device, connecting pipe is provided with between delivery eluting solvent transmitting device and eluting solvent storage device, four-way valve is provided with between connecting pipe and eluting solvent storage device, described sample feeding device is connected with waste liquid effuser.It is simple that the utility model has structure, can realize the advantage of simultaneously phthalic ester and metabolic product thereof being carried out to ON-LINE SEPARATION detection.
Description
Technical field
The utility model relates to the pretreating device of a kind of ON-LINE SEPARATION phthalic ester and metabolic product thereof, especially utilizes the mixed type integral post of being modified by two kinds of heterogeneity carbon nano-fibers biological sample to be realized to a kind of carbon nano-fiber mixed type integral post pretreatment unit of ON-LINE SEPARATION.
Background technology
Phthalic ester is a kind of widely used environment incretion interferent, in the body fluid such as human urine, blood and milk, detects phthalic ester and metabolic product thereof.Phthalic ester and metabolic product thereof have very strong toxicity for reproductive system.But because phthalic ester and metabolic product thereof have different levels, be difficult to be separated simultaneously.
At present known from biological sample isolation of purified phthalic ester and metabolic product method thereof be use solid phase column.Solid phase column is purification separation technology the most frequently used in biological sample analysis.But relative to isolation of purified technology fast and accurately, Sample Pretreatment Technique be consuming time, expend, loaded down with trivial details process.These problems significantly limit the development of analytical chemistry.For the biological sample of complexity, because sample composition is complicated, polarity scope is widely distributed, many solid phase column cannot carry out isolation of purified to the compound of opposed polarity simultaneously, and property material instrumental conditions not at the same level is different, so be separated the difficult problem that the trace that detects opposed polarity or trace constituent have become whole analytic process in the biological sample of complexity simultaneously.In recent years, although there was sample pretreatment device, these sample pretreatment device complex structures, and cannot realize carrying out ON-LINE SEPARATION testing goal to phthalic ester and metabolic product thereof simultaneously.
Summary of the invention
The purpose of this utility model overcomes the deficiencies in the prior art and provides a kind of structure simply, can realize a kind of carbon nano-fiber mixed type integral post pretreatment unit simultaneously phthalic ester and metabolic product thereof being carried out to ON-LINE SEPARATION detection.
The purpose of this utility model is achieved in that it comprises carbon nano-fiber separating column, two ends are connected to sample feeding device and detecting device with carbon nano-fiber separating column, the other end of described sample feeding device is provided with delivery eluting solvent transmitting device, delivery eluting solvent transmitting device is provided with eluting solvent storage device, connecting pipe is provided with between delivery eluting solvent transmitting device and eluting solvent storage device, be provided with four-way valve between connecting pipe and eluting solvent storage device, described sample feeding device is connected with waste liquid effuser.
Be filled with two kinds of heterogeneity carbon nano-fibers in described carbon nano-fiber separating column and modify mixed type post, a part is carbon nano-fiber, another part is modification activities carbon nano-fiber, the monofilament of carbon nano-fiber modification mixed type post is 6500 ~ 7500, filament diameter is 6 ~ 8 microns, carbon nano-fiber is modified outside mixed type post and is surrounded by polyfluortetraethylene pipe line, carbon nano-fiber is modified mixed type post two ends and is respectively equipped with polyetheretherketone joint, and described active nano carbon fiber is the reactive group being modified band aerobic by chemical modification method on carbon nano-fiber.
Described detecting device is ultraviolet detector.
Described delivery eluting solvent transmitting device is syringe pump.
Described eluting solvent storage device comprises two vials be located on syringe pump, two vials is respectively equipped with upper left pipe and upper right pipe, and the other end of upper left pipe and upper right pipe is all on four-way valve.
Owing to adopting technique scheme, a kind of carbon nano-fiber mixed type integral post pretreatment unit tool that the utility model provides has the following advantages: structure is simple, can realize carrying out ON-LINE SEPARATION detection to phthalic ester and metabolic product thereof simultaneously.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation that in the utility model, carbon nano-fiber modifies mixed type post.
In the drawings, 1, carbon nano-fiber separating column, 2, sample feeding device, 3, ultraviolet detector, 4, syringe pump, 5, connecting pipe, 6, four-way valve, 7, waste liquid effuser, 8, carbon nano-fiber, 9, active nano carbon fiber, 10, teflon pipeline, 11, polyetheretherketone joint, 12, vial, 13, upper left pipe, 14, upper right pipe.
Embodiment
As shown in Figure 1 to Figure 2, a kind of carbon nano-fiber mixed type integral post pretreatment unit that the utility model provides comprises carbon nano-fiber separating column 1, carbon nano-fiber separating column 1 two ends are connected to sample feeding device 2 and ultraviolet detector 3, sample feeding device 2 adopts six-way valve manual injector, the other end of described sample feeding device 2 is provided with delivery eluting solvent transmitting device, described delivery eluting solvent transmitting device is syringe pump 4, ensure flow speed stability in elution process, and accurate Control release flow velocity; Delivery eluting solvent transmitting device is provided with eluting solvent storage device, connecting pipe 5 is provided with between delivery eluting solvent transmitting device and eluting solvent storage device, be provided with four-way valve 6 between connecting pipe 5 and eluting solvent storage device, switch the wash-out stream of different eluting solvent; Described sample feeding device 2 is connected with waste liquid effuser 7.Be filled with two kinds of heterogeneity carbon nano-fibers in described carbon nano-fiber separating column and modify mixed type post, a part is carbon nano-fiber 8, another part is modification activities carbon nano-fiber 9, the monofilament of carbon nano-fiber modification mixed type post is 6500 ~ 7500, filament diameter is 6 ~ 8 microns, carbon nano-fiber is modified outside mixed type post and is surrounded by polyfluortetraethylene pipe line 10, carbon nano-fiber is modified mixed type post two ends and is respectively equipped with polyetheretherketone joint 11, and described active nano carbon fiber is the reactive group being modified band aerobic by chemical modification method on carbon nano-fiber.Described eluting solvent storage device comprises on two vials, 12, two vials 12 of being located on syringe pump 4 and is respectively equipped with upper left pipe 13 and upper right pipe 14, and the other end of upper left pipe 13 and upper right pipe 14 is all on four-way valve 6.
The carbon nano-fiber be filled with in carbon nano-fiber separating column loads closely thus ensures that this carbon nano-fiber post has larger column capacity and high pressure resistant character, this post external diameter is consistent with the external diameter of liquid chromatography pipeline used, the pipeline of filling carbon nano-fiber is teflon pipeline 10, thus ensure that pretreatment column easily cuts, be easy to make, the length of pretreatment column can experimentally demand be selected voluntarily; Carbon nano-fiber mixed type post two ends are respectively arranged with polyetheretherketone joint 11, thus ensure, with the suitability of whole system, to ensure minimum dead volume; Carbon nano-fiber separating column 1 is connected with UV-detector 3, detection signal, can observe the appearance time of sample.
During concrete use, for the urine sample to mouse, illustrate experimentation.1. urine extraction liquid is injected into six-way valve manual injector by micro syringe.2. open the syringe pump 4 controlling eluting solvent, deliver the eluting solvent be stored in vial 12.3. sample enters in carbon nano-fiber separating column 1 under the drive of eluting solvent, in sample the phthalic ester of opposed polarity and metabolic product different from the mechanism of action of active nano carbon fiber and carbon nano-fiber in post, optionally be retained in respectively on different pretreatment columns, described eluting solvent is the solvent of opposed polarity, is the mixed solvent of water, methyl alcohol, methylene chloride; 4. then continue to use syringe pump 4 and vial 12, deliver eluting solvent successively and carry out wash-out; 5. the phthalic ester metabolin of polar component and the phthalic ester of nonpolarity element enter in UV-detector 3 with different elution times, carry out on-line checkingi; 6. each component of device outflow after testing, can directly enter next step liquid-phase chromatographic analysis without any process.
Claims (5)
1. a carbon nano-fiber mixed type integral post pretreatment unit, it is characterized in that, it comprises carbon nano-fiber separating column, two ends are connected to sample feeding device and detecting device with carbon nano-fiber separating column, the other end of described sample feeding device is provided with delivery eluting solvent transmitting device, delivery eluting solvent transmitting device is provided with eluting solvent storage device, connecting pipe is provided with between delivery eluting solvent transmitting device and eluting solvent storage device, four-way valve is provided with between connecting pipe and eluting solvent storage device, described sample feeding device is connected with waste liquid effuser.
2. carbon nano-fiber mixed type integral post pretreatment unit according to claim 1, it is characterized in that, be filled with two kinds of heterogeneity carbon nano-fibers in described carbon nano-fiber separating column and modify mixed type post, a part is carbon nano-fiber, another part is modification activities carbon nano-fiber, the monofilament that carbon nano-fiber modifies mixed type post is 6500 ~ 7500, filament diameter is 6 ~ 8 microns, carbon nano-fiber is modified outside mixed type post and is surrounded by polyfluortetraethylene pipe line, carbon nano-fiber is modified mixed type post two ends and is respectively equipped with polyetheretherketone pipe joint, described active nano carbon fiber is the reactive group being modified band aerobic by chemical modification method on carbon nano-fiber.
3. a kind of carbon nano-fiber mixed type integral post pretreatment unit according to claim 1, it is characterized in that, described detecting device is ultraviolet detector.
4. a kind of carbon nano-fiber mixed type integral post pretreatment unit according to claim 1, it is characterized in that, described delivery eluting solvent transmitting device is syringe pump.
5. a kind of carbon nano-fiber mixed type integral post pretreatment unit according to claim 1, it is characterized in that, described eluting solvent storage device comprises two vials be located on syringe pump, two vials are respectively equipped with upper left pipe and upper right pipe, the other end of upper left pipe and upper right pipe is all on four-way valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520153487.7U CN204462089U (en) | 2015-03-18 | 2015-03-18 | A kind of carbon nano-fiber mixed type integral post pretreatment unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520153487.7U CN204462089U (en) | 2015-03-18 | 2015-03-18 | A kind of carbon nano-fiber mixed type integral post pretreatment unit |
Publications (1)
| Publication Number | Publication Date |
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| CN204462089U true CN204462089U (en) | 2015-07-08 |
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| CN201520153487.7U Expired - Fee Related CN204462089U (en) | 2015-03-18 | 2015-03-18 | A kind of carbon nano-fiber mixed type integral post pretreatment unit |
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| CN (1) | CN204462089U (en) |
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2015
- 2015-03-18 CN CN201520153487.7U patent/CN204462089U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Yanbian Kechuang ultrasonic equipment Technology Development Co Ltd Assignor: Yanbian University Contract record no.: 2015220000036 Denomination of utility model: Pretreatment device with carbon nanofibre mixed type monolithic columns Granted publication date: 20150708 License type: Exclusive License Record date: 20150807 |
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| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150708 Termination date: 20180318 |