CN204434625U - Constant temperature rotary device - Google Patents

Constant temperature rotary device Download PDF

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Publication number
CN204434625U
CN204434625U CN201420774036.0U CN201420774036U CN204434625U CN 204434625 U CN204434625 U CN 204434625U CN 201420774036 U CN201420774036 U CN 201420774036U CN 204434625 U CN204434625 U CN 204434625U
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China
Prior art keywords
conditioning unit
temperature
temperature conditioning
constant temperature
unit
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Withdrawn - After Issue
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CN201420774036.0U
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Chinese (zh)
Inventor
洪瑞贤
陈庭轩
温国兴
巫秉融
蔡秀娟
宋致祥
刘振凰
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Industrial Technology Research Institute ITRI
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Industrial Technology Research Institute ITRI
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Abstract

The utility model discloses a constant temperature rotary device, include: the first temperature control unit is used for heating and controlling the temperature of at least one reaction mixture arranged in the first temperature control unit, and an optical window and a light inlet hole are arranged outside each reaction mixture; the second temperature control unit is connected with the first temperature control unit and enables the reaction mixture to carry out amplification reaction and carry out reaction in a constant temperature environment; the rotating unit extends a driving shaft to be connected with the first temperature control unit and the second temperature control unit so as to enable the first temperature control unit and the second temperature control unit to rotate; the light source and optical detection unit emits light to the light inlet of the first temperature control unit and then to the reaction mixture, and light penetrating through the reaction mixture passes through the optical window and is received by the optical detection unit; and a control unit for controlling the first temperature control unit, the second temperature control unit, the rotation unit and the light source and optical detection unit to operate. The utility model discloses can reach the mechanism of stabilizing emitting diode's luminous power.

Description

Constant temperature swivel arrangement
Technical field
The utility model relates to a kind of constant temperature swivel arrangement, and especially relating to one provides an actuator to drive copper cavity to rotate, can in the process rotated simultaneously control chamber temperature capture the fluorescent signal of each root test tube in cavity.
Background technology
In Molecular Detection market, Polymerase Chain Reaction (Polymerase chain reaction, PCR) be the most widely used current nucleic acid amplification mode, two kinds of technology probably can be divided into: the first needs gradient of temperature to circulate (thermal cycling), and constant-temperature amplification (Isothermal amplification) technology that the second needs constant temperature to operate is to complete amplified reaction according to temperature of reaction condition.The latter is owing to being carry out target nucleic acid amplification under constant temperature, so need traditional thermal cycler unlike the former, total system can be more efficient again than Polymerase Chain Reaction system simplification.
Molecular diagnostic system in the market take macrotype platform as main flow, gradient of temperature circulation (Thermal cycling) mode of many employings the first technology above-mentioned carries out nucleic acid amplification, and to be clinical sample take a long time to the whole detection process need of result its shortcoming.The utility model institute generator, for adopting above-mentioned the second technology, take constant temperature nucleic acid amplification detection design, reagents simplifies detection system platform requirement, the amplification of multitube target nucleic acid can be carried out under constant temperature simultaneously, comparatively the first technology can Reaction time shorten, and has sufficiently high sensitivity.In more extendible detecting Multi-example number in future (multiplex), the molecular diagnostic system continued toward miniaturization develops.
Utility model content
Based on the disappearance solving the above prior art, the utility model is a kind of constant temperature swivel arrangement, its main purpose is to utilize an actuator to drive the copper cavity of the first temperature conditioning unit to rotate, can in the process rotated control chamber temperature capture the fluorescent signal of each root test tube in cavity simultaneously.First application simulation software carries out the heat biography analysis of cavity configuration, and that analyzes each root test tube thus is heated evenly degree, and meets follow-up measurement repeatability and reproducible requirement.Next designs and develops corresponding optical module, to meet fluorescence volume gauge lattice.Continue and be devoted to exploitation periphery mechanism and circuit design module.For realizing Parallel testing and avoiding the signal errors between hyperchannel, adopt single photodiode (LED) light source and single complementary metal oxide semiconductor (CMOS) detector, replace optical scanning in the mode of rotated sample, reach fast accurate location.
Another object of the utility model is to provide one first temperature conditioning unit to carry out driven rotary by motor, therefore in rotation should holding temperature constant, avoid in mechanism interference again, utilize two pairs of copper rods and its respective carter, solve coiling problem by sliding contact.
Another object of the present utility model is that the first temperature conditioning unit arranges the heating of an annular guide channel for copper cavity, module is mainly divided into two parts, first is the heat block (as shown in Figure 2) of the copper cavity of main body octagon of the first temperature conditioning unit, second is the second temperature conditioning unit (as shown in Figure 5) above the first temperature conditioning unit, both settle a temperature-sensitive sticker as the feedback device of cavity, there is provided thermostatic control simultaneously, 8 pipe reagent in the copper cavity of the first temperature conditioning unit are reacted under the constant temperature of 65 DEG C.In addition upper cover part also designs 60 DEG C of heat preservation modules, to avoid in vitro producing water vapour.
Another object of the present utility model is the metal block above the copper cavity of the first temperature conditioning unit, then that use one heating module (Heating Module) drives, the temperature-sensitive sticker (Sensor) of a heating piece and a film-type is pasted in this case inside this metal block, come coated with a bakelite material again outside this metal block, be provided with the height of a screw thread adjustable heating location between this metal block and this bakelite, this heating module carrys out the height of design temperature by a knob; Or change the parameter that a variable-resistance resistance size adjusts temperature setting.
Another object of the present utility model is in the heating module for the copper cavity of octagon of the first temperature conditioning unit, one controller (controller) is set and an amplifier (amplifier) drives, simultaneously and use rotary temperature-sensitive sticker (thermopair mode exports) to detect the change of temperature.This heating module can control the parameter of the operations such as every temperature height, the rate of rise by the interface program of computer end, to maintain the homo(io)thermism in rotation, and avoid the interference in mechanism.
For reaching above-mentioned purpose, the utility model is a kind of constant temperature swivel arrangement, and it includes:
First temperature conditioning unit, controlled in order to heat at least one reaction mixture be arranged in this first temperature conditioning unit and to do temperature, each reaction mixture outer setting has an optical window and an incidence hole;
Second temperature conditioning unit, is connected with this first temperature conditioning unit, and makes this reaction mixture carry out amplified reaction and react under isoperibol;
Rotary unit, extends a drive shaft and is connected with this first temperature conditioning unit and this second temperature conditioning unit, does a spinning movement to make this first temperature conditioning unit and this second temperature conditioning unit;
Light source and optical detecting unit, to this reaction mixture place after a luminous light source to this light inlet of this first temperature conditioning unit, the light that this reaction mixture is stimulated, by after this optical window, is received by an optical detecting unit; And
Control unit, in order to control this first temperature conditioning unit, this second temperature conditioning unit, this rotary unit and this light source and the start of optical detecting unit.
This first temperature conditioning unit also includes a well heater.
This well heater is outside the main body of this first temperature conditioning unit, paste a heating film form.
This first temperature conditioning unit is made up of an opaque heat-conducting.
Outside this first temperature conditioning unit connect below a rotating disk and be also provided with an annular guide channel, this annular guide channel can for multiple conductive pole contact, and those conductive poles are in order to for should the first temperature conditioning unit required electric power.
This conductive pole is by arranging a conduction projection in an insulating cylinder and an elastomerics formed.
This light source and optical detecting unit include a single light source and a single optical detecting unit.
This single light source is formed by a photodiode and a spacer.
This single optical detecting unit adopts non-conductive, light tight material is made.
This optical window is formed by a perforation or lens.
A filter is at least provided with between this single light source and this single optical detecting unit.
The main body of this first temperature conditioning unit is made up of copper material metal.
The main body of this first temperature conditioning unit is a polyhedron.
This polyhedron is hexagon or octet.
One first pedestal is also provided with between this first temperature conditioning unit and this second temperature conditioning unit.
This second temperature conditioning unit and this first pedestal mode of connection for screwing togather, and adjust the spacing of this first and second temperature conditioning unit by screwing togather the depth.
Also be connected with one second pedestal above this second temperature conditioning unit, this second pedestal and this second temperature conditioning unit mode of connection are for screwing togather.
This second pedestal is also connected with a temperature feedback device, in order to feedback to this control unit by detected Temperature numerical, this second pedestal and this temperature feedback device mode of connection are for screwing togather.
This rotary unit is also connected with a locating device, and this locating device is in order to control the closed position of this first temperature conditioning unit and this second temperature conditioning unit, so that this optical detecting unit does a reception.
Local protuberance in the rotating disk outer rim that this locating device is connected with this first temperature conditioning unit, as return-to-zero to produce positioning action.
This optical detecting unit is made up of an image sensor.
This rotary unit is formed for a step motor.
This reaction mixture is placed in a test tube hole of this first temperature conditioning unit.
The utility model has the advantage of; this constant temperature swivel arrangement has the function of protection inner member; also there is the temperature making overall test tube all remain consistent; avoid external environment to cause the temperature difference, and the liquid evaporation in test tube can be prevented and cause unexpected measurement, change photodiode output power size; to reach the mechanism of the luminous power of stabilized illumination diode; the structure of this case is relatively simple, and manufacturing cost is lower, is a kind of comparatively economic matured product.
Accompanying drawing explanation
Fig. 1 is the three-dimensional appearance schematic diagram of the utility model constant temperature rotatable heater;
Fig. 2 is the perspective view after the utility model constant temperature rotatable heater removing outer hull;
Fig. 3 is conduction and the optical detecting structural representation of the utility model constant temperature rotatable heater;
Fig. 4 is conduction and the rotational structure schematic diagram of the utility model constant temperature rotatable heater;
Fig. 5 is the heating arrangement schematic diagram of the utility model constant temperature rotatable heater;
Fig. 6 A is the volume rendering structural representation of the utility model conductive pole;
Fig. 6 B is the stereo decomposing structural representation of the utility model conductive pole;
Fig. 7 is the utility model light source stereo decomposing structural representation;
Fig. 8 is obtained response curve figure in similarity condition is lower by the utility model constant temperature rotatable heater and commercially available merchant.
Nomenclature
11 ~ the first housings
12 ~ the second housings
121 ~ closed cover
13 ~ three housing
2 ~ the first temperature conditioning units
21 ~ main body
211 ~ test tube hole
212 ~ heating film
213 ~ incidence hole
214 ~ optical window
22 ~ rotating disk
221 ~ annular guide channel
23 ~ the first pedestals
3 ~ the second temperature conditioning units
31 ~ the second pedestals
4 ~ rotary unit
41 ~ drive shaft
51 ~ light source
511 ~ spacer
512 ~ photodiode
513 ~ filter
52 ~ optical detecting unit
521 ~ filter
6 ~ conductive pole
61 ~ insulating cylinder
62 ~ conduction projection
63 ~ elastomerics
7 ~ locating device
8 ~ temperature feedback device
Embodiment
Refer to shown in Fig. 1; it is the three-dimensional appearance schematic diagram of the utility model constant temperature rotatable heater; wherein the first housing 11, second housing 12 and closed cover 121 internal space; be provided with the first temperature conditioning unit, the second temperature conditioning unit, light source and optical detecting unit and control unit; this the first housing 11, second housing 12 and closed cover 121 are except having the function of protection inner member; also there is the temperature making overall test tube all remain consistent; avoid external environment to cause the temperature difference, and the liquid evaporation in test tube can be prevented and cause unexpected measurement.3rd housing 13 internal space is then provided with rotary unit, and the 3rd housing 13 also has the effect of protection, to form outward appearance of the present utility model.
Refer to shown in Fig. 2 to Fig. 5, it is the structural representation of the utility model constant temperature rotatable heater, include: one first temperature conditioning unit 2, controlled in order to heat at least one reaction mixture be arranged in this first temperature conditioning unit 2 and to do temperature, this reaction mixture is placed in (the present embodiment is that use eight test tubes are placed in this test tube hole 211) in a test tube hole 211 of this first temperature conditioning unit 2, each reaction mixture outer setting has optical window 214 and an incidence hole 213, this optical window 214 is formed by a perforation or lens, this first temperature conditioning unit 2 also includes a well heater, one second temperature conditioning unit 3, is connected with this first temperature conditioning unit 2, and makes this reaction mixture carry out amplified reaction and react under isoperibol, one rotary unit 4, extend a drive shaft 41 to be connected with this first temperature conditioning unit 2 and this second temperature conditioning unit 3, to make this first temperature conditioning unit 2 and this second temperature conditioning unit 3 do a spinning movement, this rotary unit 4 is formed for a step motor (Step Motor), one light source 51 and optical detecting unit 52, to this reaction mixture place after a luminous light source 51 to this light inlet 213 of this first temperature conditioning unit 2, penetrate the light of this reaction mixture,, received by optical detecting unit 52 through optical window 214 by a smooth medium (the present embodiment is for making apparatus fluorescent reaction thing), obtain a fluorescence volume measured value, this optical detecting unit 52 is made up of an image sensor, one control unit (not shown), in order to control this first temperature conditioning unit 2, this second temperature conditioning unit 3, this rotary unit 4 and this light source 51 and optical detecting unit 52 start.
The control method of this control unit above-mentioned is: in System Operation process, maintain electric power for making light source 51 and export the stable of (Power Output), therefore take the back coupling of photodiode to control (LEDFeedback) control, photodiode controls adjustment Output optical power intensity by control unit (Control Circuit), and place an image detecting unicircuit (Photo-Detector Integrated Circuit in the side perforate of light source mechanism, PDIC) light intensity of photodiode is detected, voltage signal is converted to after the photoelectric current of image detecting unicircuit flows through the circuit for detecting (Detection Circuit) of rear end, the size of this optical signal is differentiated again through an analog/digital converter, when Solid Procedure calculates the power of optical signal, just the digital/analog converter via inside converts the size that voltage signal removes to control LED control circuit (LED Control Circuit) outward current a little to, and then change photodiode output power (LED Output Power) size, to reach the mechanism of the luminous power of stabilized illumination diode.
This first temperature conditioning unit 2 above-mentioned also includes a well heater, and this well heater is outside the main body 21 of this first temperature conditioning unit 2, paste a heating film 212 form.
In order to prevent rotating coiling phenomenon, the rotating disk 22 that one group of material is bakelite is fixed around test tube hole 211, an annular guide channel 221 is also provided with below this rotating disk 22, two tracks are provided to use copper ring cover and provide the generating positive and negative voltage of heating film 212 to conduct respectively, this annular guide channel 221 can supply multiple conductive pole 6 contact, and (the present embodiment is four, be respectively and be distributed in this two annular guide channels 211 in mode between two), those conductive poles 6 are in order to for should the first temperature conditioning unit 2 required electric power, this conductive pole 6 by arrange in an insulating cylinder 61 a conduction projection 62 and an elastomerics 63 form (as Fig. 6 A, shown in Fig. 6 B).
Refer to shown in Fig. 7, this light source 51 and optical detecting unit 52 include single light source 51 and a single optical detecting unit 52, this single light source 51 is formed by a photodiode 512 and a spacer 511, and this single optical detecting unit 52 adopts non-conductive, light tight material is made.At least be provided with a filter (513 or 521) between this single light source 51 and this single optical detecting unit 52, in the present embodiment, this filter 513 is arranged between two spacers, to make the specific wavelength of light source 51 by this filter 513; Or before this filter 521 is arranged at optical detecting unit 52, to make the specific wavelength that is excited by this filter 521, make optical detecting unit 52 reach effect (as shown in Figure 3, Figure 4) of optics accurate measurement.Light emitted by single light source 51, can through the reaction mixture containing fluorescein stain (such as Sybr Green), and inject optics detecting unit 52 (such as CCD, CMOS, PD camera/image sensor), the degree of nucleic acid iodine is immediately judged by signals such as the light intensities that detects.
The main body 21 of this first temperature conditioning unit 2 above-mentioned is made up of copper material metal; the main body 21 of this first temperature conditioning unit 2 is a polyhedron; and this polyhedron is hexagon or octet; and be octet cited by the present embodiment; but the utility model is not limit certainly with this; all act four limits body (square or long cuboid), ten jiaos of bodies, the polyhedron even more than 12 jiaos of bodies, all at the protection domain of this case.
The first pedestal 23 is also provided with between this first temperature conditioning unit 2 above-mentioned and this second temperature conditioning unit 3, this second temperature conditioning unit 3 and this first pedestal 23 mode of connection are for screwing togather, and by screw togather the depth adjust this first, two temperature conditioning units (2, 3) spacing, one second pedestal 31 is also connected with above this second temperature conditioning unit 3, this second pedestal 31 and this second temperature conditioning unit 3 mode of connection are for screwing togather, this second pedestal 31 is also connected with a temperature feedback device 8, in order to detected Temperature numerical is feedback to this control unit, this second pedestal 3 and this temperature feedback device 8 mode of connection are for screwing togather.
This rotary unit 4 above-mentioned is also connected with a locating device 7, this locating device 7 is in order to control the closed position of this first temperature conditioning unit 2 and this second temperature conditioning unit 3, so that this optical detecting unit 52 does a reception, local protuberance in the outer rim of the rotating disk 22 that this locating device 7 is connected with this first temperature conditioning unit 2, as return-to-zero (Home) to produce positioning action.
Refer to shown in Fig. 8, under rotary mode, use the agent prescription LambdaDNA (100ng) of same concentrations in three test tubes, obtain as above, the experimental result of figure below.The parallel comparative result of this case constant temperature swivel arrangement (upper figure) and commercially available merchant (figure below), can find out, between three pipes, there is high duplication, the response curve tool great similarity of two kinds of platforms, detection time all can be reduced in 30 minutes, but the structure of this case is relatively simple, manufacturing cost is lower, is a kind of comparatively economic matured product.

Claims (23)

1. a constant temperature swivel arrangement, is characterized in that, this constant temperature swivel arrangement includes:
First temperature conditioning unit, controlled in order to heat at least one reaction mixture be arranged in this first temperature conditioning unit and to do temperature, each reaction mixture outer setting has an optical window and an incidence hole;
Second temperature conditioning unit, is connected with this first temperature conditioning unit, and makes this reaction mixture carry out amplified reaction and react under isoperibol;
Rotary unit, extends a drive shaft and is connected with this first temperature conditioning unit and this second temperature conditioning unit, does a spinning movement to make this first temperature conditioning unit and this second temperature conditioning unit;
Light source and optical detecting unit, to this reaction mixture place after a luminous light source to this light inlet of this first temperature conditioning unit, the light penetrating this reaction mixture, by after this optical window, is received by an optical detecting unit; And
Control unit, in order to control this first temperature conditioning unit, this second temperature conditioning unit, this rotary unit and this light source and the start of optical detecting unit.
2. constant temperature swivel arrangement as claimed in claim 1, it is characterized in that, this first temperature conditioning unit also includes a well heater.
3. constant temperature swivel arrangement as claimed in claim 2, it is characterized in that, this well heater is outside the main body of this first temperature conditioning unit, paste a heating film form.
4. constant temperature swivel arrangement as claimed in claim 1, it is characterized in that, this first temperature conditioning unit is made up of an opaque heat-conducting.
5. constant temperature swivel arrangement as claimed in claim 1, it is characterized in that, outside this first temperature conditioning unit connect below a rotating disk and be also provided with an annular guide channel, this annular guide channel can for multiple conductive pole contact, and those conductive poles are in order to for should the first temperature conditioning unit required electric power.
6. constant temperature swivel arrangement as claimed in claim 5, is characterized in that, this conductive pole is by arranging a conduction projection in an insulating cylinder and an elastomerics formed.
7. constant temperature swivel arrangement as claimed in claim 1, it is characterized in that, this light source and optical detecting unit include a single light source and a single optical detecting unit.
8. constant temperature swivel arrangement as claimed in claim 7, it is characterized in that, this single light source is made up of a photodiode and a spacer.
9. constant temperature swivel arrangement as claimed in claim 7, is characterized in that, this single optical detecting unit adopts non-conductive, light tight material is made.
10. constant temperature swivel arrangement as claimed in claim 1, is characterized in that, this optical window is made up of a perforation or lens.
11. constant temperature swivel arrangements as claimed in claim 7, is characterized in that, are at least provided with a filter between this single light source and this single optical detecting unit.
12. constant temperature swivel arrangements as claimed in claim 1, it is characterized in that, the main body of this first temperature conditioning unit is made up of copper material metal.
13. constant temperature swivel arrangements as claimed in claim 1, is characterized in that, the main body of this first temperature conditioning unit is a polyhedron.
14. constant temperature swivel arrangements as claimed in claim 13, it is characterized in that, this polyhedron is hexagon or octet.
15. constant temperature swivel arrangements as claimed in claim 1, is characterized in that, be also provided with one first pedestal between this first temperature conditioning unit and this second temperature conditioning unit.
16. constant temperature swivel arrangements as claimed in claim 15, is characterized in that, this second temperature conditioning unit and this first pedestal mode of connection for screwing togather, and adjust the spacing of this first and second temperature conditioning unit by screwing togather the depth.
17. constant temperature swivel arrangements as claimed in claim 1, is characterized in that, be also connected with one second pedestal above this second temperature conditioning unit, and this second pedestal and this second temperature conditioning unit mode of connection are for screwing togather.
18. constant temperature swivel arrangements as claimed in claim 17, is characterized in that, this second pedestal is also connected with a temperature feedback device, and in order to feedback to this control unit by detected Temperature numerical, this second pedestal and this temperature feedback device mode of connection are for screwing togather.
19. constant temperature swivel arrangements as claimed in claim 1, it is characterized in that, this rotary unit is also connected with a locating device, and this locating device is in order to control the closed position of this first temperature conditioning unit and this second temperature conditioning unit, so that this optical detecting unit does a reception.
20. constant temperature swivel arrangements as claimed in claim 19, is characterized in that, the local protuberance in the rotating disk outer rim that this locating device is connected with this first temperature conditioning unit, as return-to-zero to produce positioning action.
21. constant temperature swivel arrangements as claimed in claim 1, it is characterized in that, this optical detecting unit is made up of an image sensor.
22. constant temperature swivel arrangements as claimed in claim 1, it is characterized in that, this rotary unit is made up of a step motor.
23. constant temperature swivel arrangements as claimed in claim 1, it is characterized in that, this reaction mixture is placed in a test tube hole of this first temperature conditioning unit.
CN201420774036.0U 2014-11-14 2014-12-10 Constant temperature rotary device Withdrawn - After Issue CN204434625U (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TW103220329 2014-11-14
TW103220329U TWM497669U (en) 2014-11-14 2014-11-14 Constant-temperature rotation device

Publications (1)

Publication Number Publication Date
CN204434625U true CN204434625U (en) 2015-07-01

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Application Number Title Priority Date Filing Date
CN201420774036.0U Withdrawn - After Issue CN204434625U (en) 2014-11-14 2014-12-10 Constant temperature rotary device

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TW (1) TWM497669U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105733939A (en) * 2014-11-14 2016-07-06 财团法人工业技术研究院 Constant temperature rotary device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105733939A (en) * 2014-11-14 2016-07-06 财团法人工业技术研究院 Constant temperature rotary device
CN105733939B (en) * 2014-11-14 2018-03-23 财团法人工业技术研究院 Constant temperature rotary device

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