CN204422527U - Medical experimental control chip - Google Patents
Medical experimental control chip Download PDFInfo
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- CN204422527U CN204422527U CN201320580501.2U CN201320580501U CN204422527U CN 204422527 U CN204422527 U CN 204422527U CN 201320580501 U CN201320580501 U CN 201320580501U CN 204422527 U CN204422527 U CN 204422527U
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- 239000000523 sample Substances 0.000 claims abstract description 63
- 238000000034 method Methods 0.000 claims abstract description 29
- 239000013642 negative control Substances 0.000 claims abstract description 22
- 239000013068 control sample Substances 0.000 claims abstract description 19
- 239000013641 positive control Substances 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims description 9
- 238000005516 engineering process Methods 0.000 claims description 7
- 241001465754 Metazoa Species 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 238000005192 partition Methods 0.000 claims description 5
- 206010044456 Transverse presentation Diseases 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 34
- 239000012528 membrane Substances 0.000 description 8
- 239000000020 Nitrocellulose Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 229920001220 nitrocellulos Polymers 0.000 description 4
- 229910052710 silicon Inorganic materials 0.000 description 4
- 239000010703 silicon Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 229940000406 drug candidate Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003777 experimental drug Substances 0.000 description 2
- 238000007901 in situ hybridization Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241001272567 Hominoidea Species 0.000 description 1
- 238000005576 amination reaction Methods 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000007037 hydroformylation reaction Methods 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000012309 immunohistochemistry technique Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000003908 quality control method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of medical experimental control chip, comprise sheet base, sheet base comprises sample area and places two contrast chip region of control sample.The invention also discloses a kind of using method of medical experimental control chip.Experiment sample and check sample are placed on same a slice base by the utility model medical experimental control chip, not only solve the problem that experiment lacks contrast very well, and ensure that the experiment condition of experiment sample and check sample is completely the same, make the judgement of experimental result more accurate and reliable.Check sample is fixed on chip by array way, uses same chip can meet the demand of plurality of medical Setup Experiments positive control and negative control, not only simplify experimental implementation, and greatly reduce experimental cost.
Description
Technical field
The invention belongs to biomedical technical field of measurement and test, be specifically related to medical experimental control chip.
Background technology
It has been generally acknowledged that rigorous scientific experiment research requirement arranges experiment contrast, comprise positive control or negative control, its objective is and will get rid of false negative or false positive experimental result.Positive control refers to the contrast that must occur positive findings in experiment, when experimental result and positive control are feminine gender, can judgment experiment result be false negative; Negative control refers to the contrast that must occur negative findings in experiment, when experimental result and negative control are the positive, can judgment experiment result be false positive.For the judgement of a certain experimental result, must be that positive findings appears in positive control, there is negative findings in negative control, so just can think that experimental result is accurately and reliably.Chinese patent 201320067241.9 discloses a kind of SABC and adheres to microslide, comprise rectangular sheet, the rectangular sheet back side is provided with two parallel region segmentation lines, thus rectangular sheet is divided into three regions along its long side direction: by examining number label mark zone, check plot and being subject to inspection district.By carrying out Region dividing to microslide, establish the control zone of standard, strict quality control and diagnosis division is carried out to facilitate, for promoting standardized dyeing procedure, the reliability improving immunohistochemical experiment has made contribution, make immunohistochemistry technique have more standardization, ImmunohistochemistryResults Results has more reproducibility and reliability.But if experimental procedure or experimental drug have gone out mistake, when sample and negative control are all shown as feminine gender, or sample and positive control are all shown as the positive, now also likely there is the result contrary with experiment contrast, i.e. false negative and false positive results.In addition, when medical inspection, because experiment lacks contrast, credible result degree is reduced, patient often throws doubt upon to positive detection report and repeatedly carries out the detection of disease sample to Duo Jia hospital, this not only adds patient's burden, waste hospital resources simultaneously, and add the workload of testing staff thus have influence on the sample detection of other patients.Therefore, necessary in medical experiment process positive control and negative control are set simultaneously, make the judgement of experimental result more accurately and reliably.
In addition, varied due to medical experiment, required check sample is also not quite similar, researcher is when carrying out different medical experiments, often need the different check sample of deposit and change different check samples when testing, invisiblely add the complicacy of experimental implementation and improve experimental cost.In addition, experiment sample and check sample normally separate in an experiment, really can not reach both experiment conditions so completely the same, and there is certain inconvenience when experimental result is observed, and need observation experiment sample and check sample respectively.
Summary of the invention
The technical matters that will solve of the present invention is to provide a kind of medical experimental control chip can avoiding the testing result led to errors because experimental procedure or experimental drug have gone out mistake, the check sample that another technical matters that the present invention also will solve in addition needs deposit different in a large number when being and avoiding carrying out medical experiment, save experimental cost, experimental implementation is simplified.
The present invention is achieved by the following technical solutions:
Medical experimental control chip, comprises sheet base, and sheet base comprises sample area and places two contrast chip region of control sample.
In order to obtain better technique effect, two described contrast chip region are respectively the positive control chip region of fixing positive control sample and the negative control chip region of fixing negative control sample; Described control sample is fixed in contrast chip region with dot matrix way arrangement; Control sample in described contrast chip region is feminine gender and the positive of one or more samples in the normal structure of human body or animal, cell or sample of nucleic acid; Described base also has location mark; Described location mark indicates sample position by least one vertical line and at least one horizontal line cross-partition; Described location mark is by vertical and/or horizontal numeral and/or letter designation sample position; Described base also has label area, for sample parameters record; Described base is resistant material; Described resistant material is the one in glass, film, biological membrane, silicon, poly-difluoride membranes or nitrocellulose filter; Described sheet base adopts the process of anticreep chip technology method, prevents control sample flake in experimental implementation.
The present invention also provides a kind of using method of medical experimental control chip, the steps include:
1, measuring samples is fixed on the sample area of medical experimental control chip;
2, the medical experimental control chip fixing measuring samples is carried out normal sample preparation;
3, detect and judge the experimental result of measuring samples and the positive control sample of medical experimental control chip and negative control sample;
4, when the positive control sample of the positive contrast chip region of medical experimental control chip is shown as the positive and the negative control sample of negative control chip region is shown as feminine gender, judgment experiment process and experimental result errorless;
5, the negative control sample being shown as non-positive or negative contrast chip region when the positive control sample of medical experimental control chip positive contrast chip region be shown as non-negative time, judgment experiment process and experimental result mistake, need repetition step (2) again to process measuring samples.
In order to obtain better technique effect, described control sample is fixed in contrast chip region with dot matrix way arrangement; Control sample in described contrast chip region is feminine gender and the positive of one or more samples in the normal structure of human body or animal, cell or sample of nucleic acid; Described base also has location mark; Described location mark indicates sample position by least one vertical line and at least one horizontal line cross-partition; Described location mark is by vertical and/or horizontal numeral and/or letter designation sample position; Described base also has label area, for sample parameters record; Described base is resistant material; Described resistant material is the one in glass, film, biological membrane, silicon, poly-difluoride membranes or nitrocellulose filter; Described sheet base adopts the process of anticreep chip technology method, prevents control sample flake in experimental implementation.
Experiment sample and check sample are placed on same a slice base by medical experimental control chip of the present invention, not only solve the problem that experiment lacks contrast very well, and ensure that the experiment condition of experiment sample and check sample is completely the same, make the judgement of experimental result more accurate and reliable.Check sample is fixed on chip by array way, uses same chip can meet the demand of plurality of medical Setup Experiments positive control and negative control, not only simplify experimental implementation, and greatly reduce experimental cost.
Accompanying drawing explanation
Fig. 1 is one of embodiment of the present invention structural representation;
Fig. 2 is two structural representations of the embodiment of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be further described.
Embodiment 1
A kind of medical experimental control chip, comprises sheet base 1, the flat board that sheet base 1 is made for resistant material, and concrete resistant material is the one in glass, film, biological membrane, silicon, poly-difluoride membranes or nitrocellulose filter; Sheet base 1 is provided with two contrast chip region 4,5 of sample area 3 and placement control sample, two contrast chip region are respectively the positive control chip region 4 of fixing positive control sample and the negative control chip region 5 of fixing negative control sample, and control sample is fixed in contrast chip region with dot matrix way arrangement; Control sample in contrast chip region is feminine gender and the positive of one or more samples in the normal structure of human body or animal, cell or sample of nucleic acid; Sheet base 1 also has location mark; Location mark is that at least one perpendicular location mark line 6 of cross-partition and at least one transverse presentation are put marking line 6 and formed; Location mark also can be the position being marked counter sample by vertical and/or laterally numeral and/or letter composition position mark 6 '; Sheet base 1 also has label area 2, for sample parameters record; Sheet base 1 adopts the process of anticreep chip technology method, prevents control sample flake in experimental implementation.The anticreep chip technology disposal route that sheet base 1 adopts, comprises common anticreep adhesive process (conventional adhesive comprises poly-D-lysine, 3-aminopropyl triethoxysilane/APES and gelatin etc.), the process of ion modification anticreep, the process of positive charge anticreep, the process of negative charge anticreep, hydroformylation process, silicidation and amination process.Be applicable to the anti-flake demand of the various medical experiments such as tissue staining, SABC, in situ hybridization, fluorescence in situ hybridization, liquid basal cell detection.
Embodiment 2
The using method of the medical experimental control chip described in embodiment 1, the steps include:
(1) measuring samples is fixed on the sample area 3 of medical experimental control chip;
(2) medical experimental control chip fixing measuring samples is carried out normal sample preparation;
(3) detect and judge feminine gender or the positive findings of measuring samples and the positive contrast chip region 4 of medical experimental control chip and negative control chip region 5;
(4) when the positive control sample of the positive contrast chip region 4 of medical experimental control chip is shown as the positive and the negative control sample of negative control chip region 5 is shown as feminine gender, judgment experiment process and experimental result errorless;
(5) negative control sample being shown as non-positive or negative contrast chip region 5 when the positive control sample of medical experimental control chip positive contrast chip region 4 be shown as non-negative time, judgment experiment process and experimental result mistake, need repetition step (2) again to process measuring samples.
Above-mentioned control sample is fixed in contrast chip region 4,5 with dot matrix way arrangement; Control sample in above-mentioned contrast chip region 4,5 is feminine gender and the positive of one or more samples in the normal structure of human body or animal, cell or sample of nucleic acid; Sheet above base 1 also has location mark; Location mark is at least one vertical line of cross-partition and the location mark line 6 of at least one horizontal line composition; Location mark also can be the position being marked counter sample by vertical and/or laterally numeral and/or letter composition position mark 6 '; Sheet above base also has label area 2, for sample parameters record; Sheet above base is resistant material; Above-mentioned resistant material is the one in glass, film, biological membrane, silicon, poly-difluoride membranes or nitrocellulose filter; Above-mentioned sheet base adopts the process of anticreep chip technology method, prevents control sample flake in experimental implementation.
Claims (9)
1. a medical experimental control chip, comprise sheet base, it is characterized in that: sheet base comprises sample area and places two of control sample contrast chip region, the control sample in two contrast chip region is feminine gender and/or the positive of one or more samples in the normal structure of human body or animal, cell or sample of nucleic acid.
2. medical experimental control chip as claimed in claim 1, is characterized in that: described base is resistant material.
3. medical experimental control chip as claimed in claim 1 or 2, is characterized in that: described control sample is fixed in contrast chip region with dot matrix way arrangement.
4. medical experimental control chip as claimed in claim 1 or 2, is characterized in that: described base also has location mark.
5. medical experimental control chip as claimed in claim 4, is characterized in that: described location mark is put marking line cross-partition by least one perpendicular location mark line and at least one transverse presentation and indicated sample position.
6. medical experimental control chip as claimed in claim 4, is characterized in that: described location mark is by vertical and/or horizontal numeral and/or letter designation sample position.
7. medical experimental control chip as claimed in claim 1 or 2, is characterized in that: described base also has label area, for sample parameters record.
8. medical experimental control chip as claimed in claim 1, is characterized in that: two described contrast chip region are respectively the positive control chip region of fixing positive control sample and the negative control chip region of fixing negative control sample.
9. medical experimental control chip as claimed in claim 1 or 2, is characterized in that: described base adopts the process of anticreep chip technology method, prevents control sample flake in experimental implementation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320580501.2U CN204422527U (en) | 2013-09-18 | 2013-09-18 | Medical experimental control chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320580501.2U CN204422527U (en) | 2013-09-18 | 2013-09-18 | Medical experimental control chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204422527U true CN204422527U (en) | 2015-06-24 |
Family
ID=53472884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320580501.2U Expired - Fee Related CN204422527U (en) | 2013-09-18 | 2013-09-18 | Medical experimental control chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204422527U (en) |
-
2013
- 2013-09-18 CN CN201320580501.2U patent/CN204422527U/en not_active Expired - Fee Related
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150624 |
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| CF01 | Termination of patent right due to non-payment of annual fee |