CN204405545U - A kind of automatic focusing flexible biological probe - Google Patents
A kind of automatic focusing flexible biological probe Download PDFInfo
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- CN204405545U CN204405545U CN201420816483.8U CN201420816483U CN204405545U CN 204405545 U CN204405545 U CN 204405545U CN 201420816483 U CN201420816483 U CN 201420816483U CN 204405545 U CN204405545 U CN 204405545U
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Abstract
The utility model relates to a kind of automatic focusing flexible biological probe, comprises LASER Light Source, micro-fluidic chip, micro-fluidic chip sense channel, detects light path fixed lens and detect light path zoom lens, dichroscope and computing machine.The automatic focusing of the laser co-focusing induced fluorescence detection of micro-fluidic chip, the depth information of Laser Focusing hot spot is obtained by Binocular Vision Principle, and the focal length of feedback regulation zoom system, pancreatic system, the focal beam spot of laser induced fluorescence detector is focused on and detects in raceway groove, improve the repeatability detected.
Description
Technical field
The present invention relates to a kind of automatic focusing device of the laser co-focusing induced fluorescence detection for micro-fluidic chip, especially utilize stereovision technique to regulate zoom lens focal length to realize the adjustment of excitation source focal beam spot, improve the repeatability of fluoroscopic examination.
Background technology
Laser co-focusing induced fluorescence detection technology is a kind of common technology in micro-fluidic chip optical detective technology, and this technology utilizes focusing principle altogether to prevent impurity signal to the interference of fluorescent signals, drastically increases the sensitivity of detecting device.But, due to the restriction of micro-fluidic chip processing technology, unavoidably there is certain mismachining tolerance in the size of micro-fluidic chip, causes Laser Focusing hot spot to there is error with mating between sense channel, limits repeatability when laser Induced Fluorescence Technology uses and reliability.
Summary of the invention
The object of the invention is to provide a kind of automatic focusing device for micro-fluidic chip induced fluorescence detection, the depth information of Laser Focusing hot spot is obtained by Binocular Vision Principle, and the focal length of feedback regulation zoom system, pancreatic system, the focal beam spot of laser induced fluorescence detector is focused on and detects in raceway groove, improve the repeatability detected.
According to a kind of automatic focusing device for micro-fluidic chip induced fluorescence detection of the present invention, comprise LASER Light Source, micro-fluidic chip, micro-fluidic chip sense channel, detect light path fixed lens and detect light path zoom lens, dichroscope and computing machine; The light of described LASER Light Source outgoing enters via dichroscope reflection and detects light path fixed lens and detect light path zoom lens, thus makes the focal beam spot of laser induced fluorescence detector focus on the sense channel formed on micro-fluidic chip;
In described micro-fluidic chip plane of incidence direction, have relative to the plane of incidence tilted-putted a pair stereoscopic vision lens respectively, the focal beam spot of LASER Light Source and micro-fluidic chip sense channel image on imageing sensor by described stereoscopic vision lens, and computing machine calculates the focal beam spot of LASER Light Source and the three-dimensional position difference of micro-fluidic chip sense channel according to the image obtained.
The array mode that the object lens of common laser co-focusing induced fluorescence detection are changed into fixed lens and zoom lens by the present invention forms and detects light path, and makes it to possess long reach and high-NA; And form binocular solid system in the objective lens and imageing sensor detecting the long reach that the placement of light path symmetria bilateralis is tilted; The optical parametric obtaining binocular solid system is demarcated before detecting, by stereoscopic vision lens the focal beam spot of LASER Light Source and micro-fluidic chip sense channel imaged on imageing sensor, computing machine calculates the focal beam spot of LASER Light Source and the three-dimensional position difference of micro-fluidic chip sense channel according to acquisition image, regulate the focal length detecting light path zoom lens to make both overlap, improve the detection repeatability of system.
Accompanying drawing explanation
For above-mentioned purpose of the present invention, feature and advantage more can be become apparent, special embodiment below, and coordinate accompanying drawing, be described in detail as follows:
Fig. 1 is according to structure of the detecting device figure of the present invention.
Wherein: LASER Light Source 1, imageing sensor 2, stereoscopic vision lens 3, micro-fluidic chip 4, micro-fluidic chip sense channel 5, detection light path fixed lens 6, light path zoom lens 7 is detected, dichroscope 8.
Embodiment
Below in conjunction with accompanying drawing and example, the present invention is done and further describes in detail.At this, schematic description and description of the present invention is for explaining the present invention, but not as a limitation of the invention.
This device comprises LASER Light Source 1, a pair imageing sensor 2, a pair stereoscopic vision lens 3, micro-fluidic chip 4, micro-fluidic chip sense channel 5, detection light path fixed lens 6 and detects light path zoom lens 7, dichroscope 8 and computing machine (not shown).The light of described LASER Light Source 1 outgoing enters via dichroscope 8 reflection and detects light path fixed lens 6 and detect light path zoom lens 7, thus realizes being incident to micro-fluidic chip 4 to induce fluorescence, and micro-fluidic chip 4 is formed sense channel 5; On micro-fluidic chip 4 plane of incidence direction, a pair stereoscopic vision lens 3 in both sides respectively slant setting to realize stereoscopic vision, by light imaging on imageing sensor 2 thereafter that stereoscopic vision lens 3 are collected.
The optical parametric of two groups of stereoscopic vision lens 3 is obtained before the device is to be used by binocular vision calibration method, during use, the image of micro-fluidic chip 4 is imaged on corresponding imageing sensor 2 through a pair stereoscopic vision lens 3, the raceway groove image that imageing sensor 2 obtains by computing machine carries out Stereo matching, LASER Light Source 1 is also obtained by computing machine through dichroscope 8 and the focal beam spot after detecting light path fixed lens 6 and detecting light path zoom lens 7 and calculates simultaneously, computing machine controls the focal length variations detecting light path zoom lens 7, regulate the focal position of LASER Light Source 1 in micro-fluidic chip sense channel 5, it is made to focus on the center of micro-fluidic chip sense channel 5, thus improve the repeatability of induced with laser detection.
Preferably, detect light path fixed lens 6 and detect the optical system that light path zoom lens 7 all adopts long reach and high-NA; Stereoscopic vision lens 3 are the objective lens of long reach.
The above is only embodiments of the invention, not does any pro forma restriction to structure of the present invention.Every above embodiment is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all still belong in the scope of technical scheme of the present invention.
Claims (2)
1., for an automatic focusing device for micro-fluidic chip induced fluorescence detection, comprise LASER Light Source (1), micro-fluidic chip (4), micro-fluidic chip sense channel (5), detect light path fixed lens (6) and detect light path zoom lens (7), dichroscope (8) and computing machine; The light of described LASER Light Source (1) outgoing enters via dichroscope (8) reflection and detects light path fixed lens (6) and detect light path zoom lens (7), thus makes the focal beam spot of laser induced fluorescence detector focus at the upper sense channel (5) formed of micro-fluidic chip (4); In described micro-fluidic chip (4) plane of incidence direction, have relative to the plane of incidence tilted-putted a pair stereoscopic vision lens (3) respectively, the focal beam spot of LASER Light Source and micro-fluidic chip sense channel (5) image on imageing sensor (2) by described stereoscopic vision lens (3), and computing machine calculates the focal beam spot of LASER Light Source (1) and the three-dimensional position difference of micro-fluidic chip sense channel (5) according to the image obtained.
2. a kind of automatic focusing device for micro-fluidic chip induced fluorescence detection according to claim 1, it is characterized in that, described detection light path fixed lens (6) and detection light path zoom lens (7) all adopt the optical system of long reach and high-NA; The objective lens that stereoscopic vision lens (3) are long reach.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420816483.8U CN204405545U (en) | 2014-12-19 | 2014-12-19 | A kind of automatic focusing flexible biological probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420816483.8U CN204405545U (en) | 2014-12-19 | 2014-12-19 | A kind of automatic focusing flexible biological probe |
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| Publication Number | Publication Date |
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| CN204405545U true CN204405545U (en) | 2015-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420816483.8U Expired - Fee Related CN204405545U (en) | 2014-12-19 | 2014-12-19 | A kind of automatic focusing flexible biological probe |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109544637A (en) * | 2018-10-18 | 2019-03-29 | 北京伟景智能科技有限公司 | Binocular calibration verifies device |
| CN112362561A (en) * | 2020-10-26 | 2021-02-12 | 南京大学 | Image processing system and method |
| CN113155791A (en) * | 2015-10-23 | 2021-07-23 | 阿贝里奥仪器有限责任公司 | Method and device for high-resolution imaging of structures marked with fluorescent markings of a sample |
-
2014
- 2014-12-19 CN CN201420816483.8U patent/CN204405545U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113155791A (en) * | 2015-10-23 | 2021-07-23 | 阿贝里奥仪器有限责任公司 | Method and device for high-resolution imaging of structures marked with fluorescent markings of a sample |
| CN109544637A (en) * | 2018-10-18 | 2019-03-29 | 北京伟景智能科技有限公司 | Binocular calibration verifies device |
| CN109544637B (en) * | 2018-10-18 | 2024-02-27 | 北京伟景智能科技有限公司 | Double-target fixed verification device |
| CN112362561A (en) * | 2020-10-26 | 2021-02-12 | 南京大学 | Image processing system and method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150617 Termination date: 20171219 |
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| CF01 | Termination of patent right due to non-payment of annual fee |