CN204400999U - Bacterial spore automatic staining instrument - Google Patents
Bacterial spore automatic staining instrument Download PDFInfo
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- CN204400999U CN204400999U CN201520034364.1U CN201520034364U CN204400999U CN 204400999 U CN204400999 U CN 204400999U CN 201520034364 U CN201520034364 U CN 201520034364U CN 204400999 U CN204400999 U CN 204400999U
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- height
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- leading screw
- bacterial spore
- slide support
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Abstract
This patent relates to a kind of bacterial spore automatic staining instrument, and the dyeing for bacterial spore makes.The technical scheme that this patent adopts is: comprise multiple heating member, also comprise permanent seat, warm table, controller, slide support, horizontal shift mechanism and height displacement mechanism; Described controller, warm table and height displacement mechanism are separately positioned on permanent seat, and heating member is evenly arranged on warm table, and described horizontal shift mechanism comprises crossbeam, displacement variable-frequency motor and displacement leading screw.The utility model is on the basis adopting traditional spore staining principle, the Color that solution exists in spore staining is poor, technical difficulty is comparatively large, human factor affects the problems such as experimental result, devise " gemma automatic staining instrument ", the bacterial spore staining of multiple sample can be operated in the short period of time, numerical control automatic staining, without the need to experimenter's hand-held and special wait, safe and efficient.
Description
Technical field
This patent relates to a kind of bacterial spore automatic staining instrument, for the making of bacterial spore staining.
Background technology
Increasing research at present confirms sporiferous bacterial strain in bacterium, and especially a lot of populations of genus bacillus have great using value in human lives, production.Spore staining from environment, screens genus bacillus carry out the requisite work of morphological observation, also be division bacteria, experimental exploration, the indispensable element task of production application, for the purpose of the further investigation of the aspect such as bacterial strain Physiology and biochemistry, heritable variation important foundation is provided.The traditional working method of spore staining has 2 kinds: method one, is first made into bacteria suspension by bacterial classification, and then by bacterium liquid and staining fluid mixing boiling water bath heating 15-20 min, then film-making, fixing dyeing are observed.The method complex operation, consuming time longer, Color is undesirable, and a lot of sporeformer is negative in dyeing, and even thalline all can not find.Method two, by bacterium on slide glass film, drying, fixing after drip dye liquor, test-tube bolder clips slide glass and is placed in above flame and heats, and dye liquor emits during steam and maintains 5 min, period dye liquor can not dry up, constantly supplement, this method Color is better.But experiment safety hidden danger is large and the difficult grasp of staining technique, because slide and spirit lamp flame distance are not easy to control, too near dye liquor evaporate to dryness soon, during supplementary dye liquor, the cold and hot sharp slide that causes mutually bursts dye liquor splashing again, pollute hand face, clothing, experiment table and surrounding environment, even operator stabbed by scald or glass cullet, and bacterial cell disruption dyes unsuccessfully.If slide distance flame is too far away, Heating temperature does not reach requirement and Color is bad, and therefore invention one spore staining safely and effectively instrument seems extremely important.
Summary of the invention
All shortcomings of corresponding prior art, this patent provides the bacterial spore automatic staining instrument of a kind of controlled heat-up time and Heating temperature.
For achieving the above object, the technical scheme that this patent adopts is:
Bacterial spore automatic staining instrument, comprise multiple heating member, it is characterized in that, also comprise permanent seat, warm table, slide support, horizontal shift mechanism, height displacement mechanism and waste collecting device, described controller, warm table and height displacement mechanism are separately positioned on permanent seat, heating member is evenly arranged on warm table, the inboardend of horizontal shift mechanism is arranged in height displacement mechanism, slide support and dropping liquid control device are arranged in horizontal shift mechanism, slide support is established and carries glass hole, carry the position of glass hole in slide support corresponding with the heating member position on warm table, waste collecting device is rotatably provided in height displacement mechanism, the rotating range of waste collecting device in height displacement mechanism is extremely perpendicular to slide support below slide support, dropping liquid control device is established two minim pipettes arranged side by side and wash tub pipe, the outlet ends of described two minim pipettes establishes air-dry apparatus.
Described horizontal shift mechanism comprises crossbeam, displacement variable-frequency motor and displacement leading screw, displacement leading screw two ends are arranged on crossbeam with rotating manner, displacement variable-frequency motor is arranged on the inboardend of crossbeam, the nut of displacement leading screw establishes described dropping liquid control device, described slide support is arranged on crossbeam, and the two ends of crossbeam are provided with horizontal position switch.
Described height running gear comprises height variable-frequency motor, vertical supporting, height leading screw, track and roller, height variable-frequency motor is arranged on vertical supporting bottom, crossbeam is fixed on the nut of height leading screw with horizontal cross position, vertical position switch is established in height leading screw upper and lower end parts position, described track is arranged on vertical supporting both sides, two rollers established by one siding track, and roller is arranged on the nut of height leading screw with rotating manner.
Described waste collecting device comprises receiving tank, rotating machine, and described rotating machine is arranged on above the nut of height leading screw, and receiving tank is fixed on the output shaft of rotating machine.
Also comprise controller, controller circuitry is connected to displacement variable-frequency motor, height variable-frequency motor, horizontal position switch, vertical position switch and heating member.
Establish the dye liquor liquid feeding peristaltic pump of two minim pipettes in described dropping liquid control device respectively, the dripping quantity of minim pipette is 100-200-μ l/ time.
The quantity of described year glass hole and heating member is respectively six.
Invent on the basis adopting traditional spore staining principle, the Color that solution exists in spore staining is poor, technical difficulty is comparatively large, human factor affects the problems such as experimental result, devise " bacterial spore automatic staining instrument ", the bacterial spore staining of multiple sample can be operated in the short period of time, numerical control automatic staining, without the need to experimenter's hand-held and special wait, safe and efficient.Achieve the function of following 3 aspects:
1. pair glass slide controls separately heating, homogeneous heating, and security is high;
2. quantitatively, timing drips the function of dye liquor to glass slide;
3. carry out the independent control automatic staining of 6 slide glasss simultaneously.
Accompanying drawing explanation
Fig. 1 is the front view of this patent,
Fig. 2 is the right view of this patent,
Fig. 3 is the vertical view of this patent.
Fig. 4 is the three-dimensional structure diagram of this patent.
1, permanent seat, 2, controller, 3, warm table, 4, heating member, 5, vertical supporting, 6, roller, 7, track, 8, height variable-frequency motor, 9, highly leading screw 9,10, vertical position switch, 11, displacement variable-frequency motor, 12, minim pipette, 13, displacement leading screw, 14, horizontal position switch, 15, slide support, 16, carry glass hole, 17, air-dry apparatus, 18, waste collection groove, 19, rotating machine.
Embodiment
This patent is further illustrated: bacterial spore automatic staining instrument, comprise multiple heating member 4, it is characterized in that, also comprise permanent seat 1, warm table 3, slide support 15, horizontal shift mechanism, height displacement mechanism and waste collecting device, described controller 2, warm table 3 and height displacement mechanism are separately positioned on permanent seat 1, heating member 4 is evenly arranged on warm table 3, the inboardend of horizontal shift mechanism is arranged in height displacement mechanism, slide support 15 and dropping liquid control device are arranged in horizontal shift mechanism, slide support 15 is established and carries glass hole 16, carry the position of glass hole 16 in slide support 15 corresponding with heating member 4 position on warm table 3, waste collecting device is rotatably provided in height displacement mechanism, the rotating range of waste collecting device in height displacement mechanism is extremely perpendicular to slide support 15 below slide support 15, dropping liquid control device is established two minim pipettes arranged side by side and wash tub pipe, the outlet ends of described two minim pipettes establishes air-dry apparatus 17.
Described horizontal shift mechanism comprises crossbeam, displacement variable-frequency motor 11 and displacement leading screw 13, displacement leading screw 13 two ends are arranged on crossbeam with rotating manner, displacement variable-frequency motor 11 is arranged on the inboardend of crossbeam, the nut of displacement leading screw 13 establishes described dropping liquid control device, described slide support 15 is arranged on crossbeam, and the two ends of crossbeam are provided with horizontal position switch 14.
Described height running gear comprises height variable-frequency motor 8, vertical supporting 5, height leading screw 9, track 7 and roller 6, height variable-frequency motor 8 is arranged on vertical supporting 5 bottom, crossbeam is fixed on the nut of height leading screw 9 with horizontal cross position, vertical position switch 10 is established in height leading screw 9 upper and lower end parts position, described track 7 is arranged on vertical supporting 5 both sides, two rollers 6 established by one siding track 7, and roller 6 is arranged on the nut of height leading screw 9 with rotating manner.
Described waste collecting device comprises receiving tank 18, rotating machine 19, and described rotating machine 19 is arranged on above the nut of height leading screw 9, and receiving tank is fixed on the output shaft of rotating machine 19.
Also comprise controller, controller circuitry is connected to displacement variable-frequency motor 11, height variable-frequency motor 8, horizontal position switch 14, vertical position switch 10 and heating member.
Establish the dye liquor liquid feeding peristaltic pump of two minim pipettes in described dropping liquid control device respectively, the dripping quantity of minim pipette 12 is 100-200 μ l/ time.
Practical function of the present invention:
1. pair glass slide controls separately heating, homogeneous heating, and security is high;
1) be by being placed on base 6 the independent heating members controlled to the heating of glass slide, dyeing needs to add heat fixation to mycoderm when starting, during dyeing, heating makes gemma painted, and dyeing terminates by the residual dye liquor of heating piece waste heat evaporation slide glass.By programdesign timing heated, temperature control scope: 100-150 DEG C, heating-surface area 56 × 20 mm.
2) slide support can hold 6 specifications is glass slide that is long by 75.25, wide 25.4mm, slide support is fixed on crossbeam, being driven by displacement variable-frequency motor can overall VTOL (vertical take off and landing), during heating, the slide glass in slide support is declined and Contact Heating sheet starts heating.
3) painting mycoderm position mark has been carried out in the glass hole of carrying of placing the slide support region of slide glass, and experimenter carries out automatic staining after can being coated with mycoderm on the corresponding position of slide glass.
2. realize quantitatively, timing drips dye liquor, the automatic cleaning of slide glass, the function that dries up fast to slide glass;
1) when heating completes, when needing to add dye liquor, cleaning, with height variable-frequency motor, slide support is drawn high liquid feeding, cleans, dries up position, with minim pipette liquid feeding, cleaning or air-dry slide glass.
2) first time dyeing, dye liquor a is by the first minim pipette conveying, minim pipette connects peristaltic pump, peristaltic pump imbibition one end connects liquid storage bottle, and displacement variable-frequency motor drives displacement leading screw to rotate, and minim pipette is carried out horizontal shift above slide glass, accurately dye liquor is added drop-wise to above slide glass specified location, by programdesign timing, quantitatively drip dye liquor, dripping quantity: 100-200 μ l/ time/sheet, dyeing 5-8 min.
3), after cooling 5-10 s, wash tub pipe water rinses the slide glass dyeed.The water-in of wash tub pipe connects water tap, and water outlet is placed in above the outside of mycoderm, by the flow of solenoid control water.When cleaning, waste liquid tank is automatically rotated to below slide support from this device left front by time variable control, collects cleaning waste liquid.
4) second time dyeing, adds dye liquor b by the second minim pipette, dyeing 2-3 min, after end to be dyed, carries out second time cleaning.
5) cleaning terminates rear automatic blow-dry slide glass, and dyeing course all completes, and gets sem observation on slide glass.
3. carry out the independent control automatic staining of 6 slide glasss simultaneously.
By the unicircuit of programming in advance, independent control is carried out to heating member, peristaltic pump, displacement and height variable-frequency motor, dropping liquid control device, air-dry apparatus, heat-up time, liquid feeding volume etc., realize the automatic staining of designated area.
Claims (7)
1. a bacterial spore automatic staining instrument, comprise multiple heating member (4), it is characterized in that, also comprise permanent seat (1), warm table (3), slide support (15), horizontal shift mechanism, height displacement mechanism and waste collecting device, described controller (2), warm table (3) and height displacement mechanism are separately positioned on permanent seat (1), described multiple heating members (4) are evenly arranged on warm table (3), the inboardend of horizontal shift mechanism is arranged in height displacement mechanism, slide support (15) and dropping liquid control device are arranged in horizontal shift mechanism, slide support (15) is established and carries glass hole (16), carry the position of glass hole (16) in slide support (15) corresponding with heating member (4) position on warm table (3), waste collecting device is rotatably provided in height displacement mechanism, the rotating range of waste collecting device in height displacement mechanism is that slide support (15) below is extremely perpendicular to slide support (15), dropping liquid control device is established two minim pipettes arranged side by side and wash tub pipe, the outlet ends of described two minim pipettes establishes air-dry apparatus (17).
2. bacterial spore automatic staining instrument according to claim 1, it is characterized in that, described horizontal shift mechanism comprises crossbeam, displacement variable-frequency motor (11) and displacement leading screw (13), displacement leading screw (13) two ends are arranged on crossbeam with rotating manner, displacement variable-frequency motor (11) is arranged on the inboardend of crossbeam, the nut of displacement leading screw (13) establishes described dropping liquid control device, described slide support (15) is arranged on crossbeam, and the two ends of crossbeam are provided with horizontal position switch (14).
3. bacterial spore automatic staining instrument according to claim 1, it is characterized in that, described height running gear comprises height variable-frequency motor (8), vertical supporting (5), height leading screw (9), track (7) and roller (6), height variable-frequency motor (8) is arranged on vertical supporting (5) bottom, crossbeam is fixed on the nut of height leading screw (9) with horizontal cross position, vertical position switch (10) is established in height leading screw (9) upper and lower end parts position, described track (7) is arranged on vertical supporting (5) both sides, two rollers (6) established by one siding track (7), roller (6) is arranged on the nut of height leading screw (9) with rotating manner.
4. bacterial spore automatic staining instrument according to claim 1 and 2, it is characterized in that, described waste collecting device comprises receiving tank (18), rotating machine (19), described rotating machine 19 is arranged on above the nut of height leading screw (9), and receiving tank is fixed on the output shaft of rotating machine (19).
5. the bacterial spore automatic staining instrument according to claim 1 or 2 or 3, it is characterized in that, also comprise controller, controller circuitry is connected to displacement variable-frequency motor (11), height variable-frequency motor (8), horizontal position switch (14), vertical position switch (10) and heating member.
6. the bacterial spore automatic staining instrument according to claim 1, is characterized in that, establishes the dye liquor liquid feeding peristaltic pump of two minim pipettes in described dropping liquid control device respectively, and the dripping quantity of minim pipette (12) is 100-200 μ l/ time.
7. the bacterial spore automatic staining instrument according to claim 1, is characterized in that, the quantity of described year glass hole (16) and heating member (4) is respectively 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520034364.1U CN204400999U (en) | 2015-01-19 | 2015-01-19 | Bacterial spore automatic staining instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520034364.1U CN204400999U (en) | 2015-01-19 | 2015-01-19 | Bacterial spore automatic staining instrument |
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| Publication Number | Publication Date |
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| CN204400999U true CN204400999U (en) | 2015-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520034364.1U Expired - Fee Related CN204400999U (en) | 2015-01-19 | 2015-01-19 | Bacterial spore automatic staining instrument |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560701A (en) * | 2015-01-19 | 2015-04-29 | 枣庄学院 | Automatic bacterial spore stainer |
| CN112697564A (en) * | 2021-01-14 | 2021-04-23 | 南京朗润新智能科技有限公司 | Gram staining method auxiliary device |
-
2015
- 2015-01-19 CN CN201520034364.1U patent/CN204400999U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560701A (en) * | 2015-01-19 | 2015-04-29 | 枣庄学院 | Automatic bacterial spore stainer |
| CN112697564A (en) * | 2021-01-14 | 2021-04-23 | 南京朗润新智能科技有限公司 | Gram staining method auxiliary device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Tan Xiaoyan Inventor after: Ma Yaohua Inventor after: Tan Xiaoyan, Ma Yaohua, Zheng Guixing Inventor before: Ma Yaohua Inventor before: Zheng Guixing Inventor before: Ma Yaohua, Zheng Guixing, Tan Xiaoyan |
|
| COR | Change of bibliographic data | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150617 Termination date: 20170119 |