CN204356323U - The outer operating gear of embryoid body - Google Patents

The outer operating gear of embryoid body Download PDF

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Publication number
CN204356323U
CN204356323U CN201420850512.2U CN201420850512U CN204356323U CN 204356323 U CN204356323 U CN 204356323U CN 201420850512 U CN201420850512 U CN 201420850512U CN 204356323 U CN204356323 U CN 204356323U
Authority
CN
China
Prior art keywords
snubber assembly
infusion tube
drying installation
embryo
embryoid body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201420850512.2U
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Chinese (zh)
Inventor
田文儒
迟世凯
齐晓楠
赵园
李华涛
丛霞
曹荣峰
姜忠玲
高善颂
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Qingdao Agricultural University
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Qingdao Agricultural University
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Publication date
Application filed by Qingdao Agricultural University filed Critical Qingdao Agricultural University
Priority to CN201420850512.2U priority Critical patent/CN204356323U/en
Application granted granted Critical
Publication of CN204356323U publication Critical patent/CN204356323U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model provides a kind of embryo manipulation device, and comprise and inhale embryo pin, infusion tube and liquid medicine filter, described suction embryo pin is arranged on one end of infusion tube, and described liquid medicine filter is arranged on the rear end of infusion tube being inhaled embryo pin.The outer operating gear of described embryoid body also comprises refining plant, snubber assembly and drying installation, one end of described snubber assembly is connected with refining plant, the other end of described snubber assembly is provided with rifle head junction surface, and described drying installation is arranged on the below of snubber assembly, and is connected with snubber assembly.Described infusion tube is communicated with refining plant by junction surface away from the one end of inhaling embryo pin; Described snubber assembly is the hollow cavity expanded that internal diameter is greater than infusion tube.The utility model not only can buffer gas flow, prevents due to firmly excessive and embryo that is that cause loses; Saliva, purifying gas flow can also be absorbed, avoid the fetal development problem because saliva causes.In addition, the utility model can autoclaving, reuses, reduces costs.

Description

The outer operating gear of embryoid body
Technical field
The invention belongs to animal reproduction and medical field, be specifically related to the embryo manipulation device in animal embryo Process of in vitro, be mainly used in the external acquisition of animal embryo, cultivation and transplanting.
Background technology
At present, in animal embryo produced in vitro technology, staff just must can complete the manipulation in vitro of embryo by embryo's suction pipe.Embryo's suction pipe is divided into mouthful suction pipe and two kinds, hand suction pipe, wherein mouth suction pipe have easy and simple to handle, quick, be convenient to the advantages such as control, by most of worker is adopted.But in use there is many defects in mouth suction pipe: in (1) operating process, often run into the situation that saliva enters embryo's suction pipe, make the dynamics drawing and blow out embryo uneven, suction is little can not reach the object drawing embryo, blowout great efforts then easily blow out bubble, easily lose embryo and cause embryonic death.In actual production, embryo is rare and precious, if just make embryo lose in embryo operation process or death, can have a strong impact on Embryo Production efficiency.(2) drawing and blowing out in the process of embryo, saliva in suction pipe is both unhygienic, may affect fetal development again, the objectionable constituent such as such as, bacterium in operator's saliva or virus when oral disease (catch a cold or), may affect fetal development.
Utility model patent CN2824871Y discloses a kind of embryo manipulation device, is made up of the cylindrical shell of kapillary and hollow form, and described cylindrical shell is cylindrical substantially, is become with sealed end head group by connecting joint, filtration chamber, silicone tube.Connecting joint is cylindrical substantially, and one end that it is connected with kapillary is cylindrical, and centre is trapezoidal zone of transition, is thereafter columniform cylindrical shell; The outer wall of the other end of connecting joint has the boss of a circle, and preset a filtration chamber in it, this room is filled with sterilized absorbent cotton; One end of silicone tube and the round boss compact siro spinning technology of described connecting joint.This utility model patent is the novelty transformation that opponent inhales embryo's pipe.At present, saliva openly not avoided in embryo operation process through the available instrument of rifle head admission port suction pipe.
Utility model content
For solving Problems existing when embryo drawn by existing mouth suction pipe, the utility model provides a kind of embryo manipulation device, and described embryo manipulation device can absorb saliva, again can buffer gas flow, can also effective filter bacteria.
The technical solution of the utility model:
The utility model one end connects the 1mL liquid-transfering gun rifle head that autoclaving is crossed, and the other end connects the infusion tube (the utility model uses liquid medicine filter and the infusion tube part of disposable transfusion device) of disposable medical transfusion device.
The outer operating gear of embryoid body, comprise and inhale embryo pin, infusion tube and liquid medicine filter, described suction embryo pin is arranged on one end of infusion tube, and described liquid medicine filter is arranged on the rear end of infusion tube being inhaled embryo pin.The outer operating gear of described embryoid body also comprises refining plant, snubber assembly and drying installation, one end of described snubber assembly is connected with refining plant, the other end of described snubber assembly is provided with rifle head junction surface, and described drying installation is arranged on the below of snubber assembly, and is connected with snubber assembly.Described infusion tube is communicated with refining plant by junction surface away from the one end of inhaling embryo pin; Described snubber assembly is the hollow cavity expanded that internal diameter is greater than infusion tube.
The end face that described drying installation is communicated with snubber assembly is provided with dismountable perforated flat plate, and the bottom face of described snubber assembly is provided with opening, only has and combines could normally use with drying installation.Filling siccative in described drying installation.Dismountable perforated flat plate is set, is convenient to change siccative.The aperture of perforated flat plate is 2 ~ 4mm, can prevent the siccative putting into moisture eliminator from being blown afloat.The utility model uses silica-gel drier, and it is cheap, good absorbing effect and harmless.For ensureing assimilation effect, preferably change siccative after each use.
The end face that described drying installation is communicated with snubber assembly is provided with slideway projection or the slideway groove of strip, and the bottom face of described snubber assembly is provided with the slideway groove or protruding with the slideway of slideway matching grooves mated with slideway projection.Described slideway groove or slideway projection are the parts that drying installation is connected with snubber assembly, recess width 2mm.
Described refining plant is the wide hollow part in narrow centre, two ends, is provided with tetrafluoroethylene millipore filtration in described refining plant; Tetrafluoroethylene millipore filtration possesses permeable watertight, resistance is low, the feature of aperture little (0.2 ~ 1.5 μm), can filter bacteria, solves conventional bacteria strainer and can not breathe freely or the problem that air permeance resistance is large.Described infusion tube joint portion is flaring hard hollow part, and described rifle head junction surface is the hard hollow part of column.Infusion tube joint portion is the part connecting infusion tube, is the outlet of cleaner.Rifle head joint portion is the position connecting 1mL liquid-transfering gun rifle head, and this position is short and hard, and after connection rifle head, rifle head tip will enter snubber 2 ~ 3mm, to ensure that saliva can directly enter in this contrive equipment.In addition, in use the utility model is in horizontality, is beneficial to and better absorbs saliva.Compared with infusion tube, snubber assembly is one and expands portion, has the effect of buffer gas flow, thus prevents due to firmly excessive and embryo that is that cause loses.
Wherein, described snubber assembly is semi-cylindrical hollow cavity, and drying installation is the hollow cavity of rectangular parallelepiped, and the combining site of described snubber assembly and drying installation realizes sealing by sealing-ring, and described sealing-ring is elastic silica gel O shape circle.After snubber assembly and drying installation combine, put sealing-ring, airtight to ensure this device.Wide 6 ~ the 8mm of sealing-ring, thick 2 ~ 3mm.
Above-mentioned each integral part, except specified otherwise, makes by polypropylene PP material.
The beneficial effects of the utility model are:
(1) the utility model is provided with snubber assembly, drying installation and refining plant, therefore, can buffer gas flow, prevent due to firmly excessive and embryo that is that cause loses; Saliva, purifying gas flow can be absorbed, avoid the fetal development problem because saliva causes;
(2) the utility model adopts polypropylene PP material to be made, can autoclaving, reuses, saving resource, reduces costs; In addition, two ends are respectively equipped with infusion tube junction surface and rifle head junction surface, the convenient replacing realizing disposable apparatus under the nonexpondable prerequisite of body;
(3) the utility model is easy and simple to handle, can significantly shorten the embryo operation time, improves the surviving rate of extracorporeal culturing embryo.
Accompanying drawing explanation
Fig. 1 is one of structural representation of the present utility model;
Fig. 2 is structural representation two (removal drying installation) of the present utility model;
Fig. 3 is the structural representation of the utility model drying installation;
Wherein, 1, infusion tube joint portion, 2, refining plant, 3, snubber assembly, 4, rifle head junction surface, 5, slideway groove, 6, opening, 7, slideway is protruding, 8, perforated flat plate, 9, through hole, 10, rifle head, 11, drying installation, 12, infusion tube.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described further.
Embodiment 1:
The outer operating gear of embryoid body, comprise and inhale embryo pin, infusion tube 12, liquid medicine filter, refining plant 2, snubber assembly 3 and drying installation 11, described suction embryo pin is arranged on one end of infusion tube 12, and described liquid medicine filter is arranged on the rear end of infusion tube 12 being inhaled embryo pin.One end of described snubber assembly 3 is connected with refining plant 2, and the other end of described snubber assembly 3 is provided with rifle head junction surface 4, and described drying installation 11 is arranged on the below of snubber assembly 3, and is connected with snubber assembly 3.Infusion tube 12 is communicated with refining plant 2 by infusion tube junction surface 1 away from the one end of inhaling embryo pin.Described snubber assembly 3 is greater than the semi-cylindrical hollow cavity expanded of infusion tube 12 for internal diameter, and drying installation 11 is hollow cavities of rectangular parallelepiped.The combining site of snubber assembly 3 and drying installation 11 realizes sealing by sealing-ring, and described sealing-ring is elastic silica gel O shape circle.After snubber assembly 3 and drying installation 11 combine, put sealing-ring, airtight to ensure this device.Wide 6 ~ the 8mm of sealing-ring, thick 2 ~ 3mm.
The end face that described drying installation 11 is communicated with snubber assembly 3 is provided with dismountable perforated flat plate 8, and the bottom face of described snubber assembly 3 is provided with opening 6, only has and combines could normally use with drying installation 11.Filling silica-gel drier in described drying installation 11.The aperture of perforated flat plate is 2 ~ 4mm, can prevent the siccative putting into moisture eliminator from being blown afloat.The end face that described drying installation 11 is communicated with snubber assembly 3 is provided with the slideway projection 7 of strip, and the bottom face of described snubber assembly 3 is provided with the slideway groove 5 mated with slideway protruding 7.Described slideway groove 5 and slideway projection 7 are parts that drying installation 11 is connected with snubber assembly 3, recess width 2mm.
Described refining plant 2 is the wide hollow part in narrow centre, two ends, is provided with tetrafluoroethylene millipore filtration in described refining plant 2.Described infusion tube joint portion 1 is flaring hard hollow part, and described rifle head junction surface 4 is the hard hollow part of column.Infusion tube joint portion 1 is the part connecting infusion tube 12, is the outlet of cleaner 2.Rifle head joint portion 4 is the positions connecting 1mL liquid-transfering gun rifle head, and this position is short and hard, and after connection rifle head, rifle head tip will enter 2 ~ 3mm in snubber assembly 3, to ensure that saliva can directly in access to plant.In addition, in use the utility model is in horizontality, is beneficial to and better absorbs saliva.Compared with infusion tube, snubber assembly is one and expands portion, has the effect of buffer gas flow, thus prevents due to firmly excessive and embryo that is that cause loses.
Wherein, above-mentioned each integral part, except specified otherwise, makes by polypropylene PP material.
The utility model one end connects the 1mL liquid-transfering gun rifle head that autoclaving is crossed, and the other end connects the infusion tube (the utility model uses liquid medicine filter and the infusion tube part of disposable transfusion device) of disposable medical transfusion device.
Concrete connection embryo suction means operation steps is as follows:
(1) intercept infusion tube: take out disposable medical tubing, front end cuts off intravenous needle, rear end cuts off drips a kettle, bottle plug puncture unit, inlet pipe air filter remove flow regulator, is required infusion tube 12.
(2) siccative is added: be encased in by proper amount of dry drying prescription in drying installation 11, then perforated flat plate 8 be arranged on drying installation 11.
(3) moisture eliminator and snubber is assembled: combined with the slideway groove 5 on snubber assembly 3 by slideway protruding 7 by the drying installation 11 that siccative is housed, the perforate 6 of snubber assembly 3 bottom face is communicated with the perforated flat plate 8 on drying installation 11 top.
(4) overlap sealing-ring: after drying installation 11 and snubber assembly 3 being connected, have elastic silica gel O-ring seals from bottom cover, assurance device is close airtight.
(5) rifle head and infusion tube is connected: 1mL liquid-transfering gun rifle head (10) crossed by autoclaving is arranged on the rifle head joint portion 4 on snubber assembly 3, require that rifle head 10 enters 2 ~ 3mm in snubber assembly 3, then directly to enter in snubber assembly if any saliva outflow when drawing embryo.The utility model assembled is inserted by infusion tube joint portion 1 in the infusion tube 12 intercepted.
(6) suction embryo pin is connected: the other end suction embryo pin being connected to infusion tube 12.
After off-test, taken apart by outer for embryoid body operating gear, after infusion tube, rifle head and siccative being changed, all the other each several parts carry out autoclaving, use in order to next time.
Embodiment 2:
As different from Example 1, the end face that described drying installation 11 is communicated with snubber assembly 3 is provided with the slideway groove of strip, and the bottom face of described snubber assembly 3 is provided with the slideway of slideway matching grooves protruding.
Embodiment 3:
As different from Example 1, described drying installation 11 is connected with snubber assembly 3 buckle.

Claims (7)

1. the outer operating gear of embryoid body, comprise and inhale embryo pin, infusion tube (12) and liquid medicine filter, described suction embryo pin is arranged on one end of infusion tube (12), described liquid medicine filter is arranged on the upper rear end of inhaling embryo pin of infusion tube (12), it is characterized in that: the outer operating gear of described embryoid body also comprises refining plant (2), snubber assembly (3) and drying installation (11), one end of described snubber assembly (3) is connected with refining plant (2), the other end of described snubber assembly (3) is provided with rifle head junction surface (4), described drying installation (4) is arranged on the below of snubber assembly (3), and be connected with snubber assembly (3), described infusion tube (12) is communicated with refining plant (2) by infusion tube junction surface (1) away from the one end of inhaling embryo pin, described snubber assembly (3) is greater than the hollow cavity expanded of infusion tube (12) for internal diameter.
2. the outer operating gear of embryoid body according to claim 1, it is characterized in that: the end face that described drying installation (11) is communicated with snubber assembly (3) is provided with dismountable perforated flat plate (8), the bottom face of described snubber assembly (3) is provided with opening (6), filling siccative in described drying installation (11).
3. the outer operating gear of embryoid body according to claim 2, it is characterized in that: the end face that described drying installation (11) is communicated with snubber assembly (3) is provided with slideway projection (7) or the slideway groove of strip, the bottom face of described snubber assembly (3) is provided with the slideway groove (5) or protruding with the slideway of slideway matching grooves mated with slideway projection (7); The aperture of described perforated flat plate (8) is 2-4mm.
4. the outer operating gear of embryoid body according to claim 2, is characterized in that: described drying installation (11) is connected with snubber assembly (3) buckle.
5. the outer operating gear of embryoid body according to claim 1, is characterized in that: described refining plant (2) is the wide hollow part in narrow centre, two ends, and described refining plant is provided with tetrafluoroethylene millipore filtration in (2); Described infusion tube joint portion (1) is flaring hard hollow part, the hard hollow part that described rifle head junction surface (4) is column.
6. the outer operating gear of embryoid body according to claim 1, it is characterized in that: described snubber assembly (3) is semi-cylindrical hollow cavity, drying installation (11) is the hollow cavity of rectangular parallelepiped, the combining site of described snubber assembly (3) and drying installation (11) realizes sealing by sealing-ring, described sealing-ring is elastic silica gel O shape circle, wide 6 ~ the 8mm of sealing-ring, thick 2 ~ 3mm.
7. the outer operating gear of embryoid body according to claim 1, is characterized in that: described refining plant (2), snubber assembly (3) and drying installation (11) all adopt polypropylene PP material.
CN201420850512.2U 2014-12-26 2014-12-26 The outer operating gear of embryoid body Expired - Fee Related CN204356323U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420850512.2U CN204356323U (en) 2014-12-26 2014-12-26 The outer operating gear of embryoid body

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420850512.2U CN204356323U (en) 2014-12-26 2014-12-26 The outer operating gear of embryoid body

Publications (1)

Publication Number Publication Date
CN204356323U true CN204356323U (en) 2015-05-27

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107858290A (en) * 2017-12-28 2018-03-30 中国医科大学 A kind of mouse ovarian separation and egg mother cell culture apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107858290A (en) * 2017-12-28 2018-03-30 中国医科大学 A kind of mouse ovarian separation and egg mother cell culture apparatus

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150527

Termination date: 20161226

CF01 Termination of patent right due to non-payment of annual fee