CN204188624U - Creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper - Google Patents
Creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper Download PDFInfo
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- CN204188624U CN204188624U CN201420645292.XU CN201420645292U CN204188624U CN 204188624 U CN204188624 U CN 204188624U CN 201420645292 U CN201420645292 U CN 201420645292U CN 204188624 U CN204188624 U CN 204188624U
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Abstract
The utility model discloses a kind of creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper, comprise base plate, sample pad, fluorescent marker pad, cellulose nitrate and adsorptive pads; Described test strips is overlapped successively to be pasted onto on base plate formed by sample pad, fluorescent marker pad, cellulose nitrate, adsorptive pads.Described fluorescent marker pad contains marked by streptavidin fluorescin and biotin labeled creatine kinase isozyme monoclonal antibody; Described nitrocellulose membrane has detection line and nature controlling line, detection line is by creatine kinase isozyme monoclonal antibody, and it has different identification epi-positions from above-mentioned biotin labeled creatine kinase isozyme monoclonal antibody.The utility model is (as: chemoluminescence method/Immune-enhancing effect turbidimetry/colloidal gold method) compared with the method for detection creatine kinase isozyme common at present, not only substantially reduces detection time, also improves detection sensitivity simultaneously.
Description
Technical field
The utility model belongs to field of immunodetection, is specifically related to a kind of high sensitivity creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper.
Background technology
Creatine kinase isozyme (Creatinekinase-MB, CK-MB) detection can be used for the diagnosis and prognosis of acute myocardial infarction AMI (AMI), myocarditis diagnosis and prognosis, the diagnosis of muscle disease, cerebral disease is as the diagnosis etc. of the diseases such as cerebral infarction, meningitis, encephalitis.
Creatine kinase (Creatinekinase, CK) is once called as cretinephosphokinase (Creatinephosphokinase, CPK).CK molecular weight is 81000, is made up of two subunits.Usually in the cytoplasm being present in the tissue such as the heart of animal, muscle and brain and mitochondria; be that one operates with intracellular energy, contraction of muscle, ATP regenerate the important kinases having direct relation, its phosphoryl that turns reversibly between catalysis creatine and ATP reacts.CK is catalysis creatine phosphate under ATP participates in, and generates ATP and phosphocreatine.This enzymic catalytic reaction is reversible.In Late Cambrian serum of patients with prostate cancer in 1979, the content of creatine kinase significantly raises, and after this in the cancer patient of other types, also has identical discovery.Along with going deep into of research, people begin through screening creatine kinase inhibitor and find new antineoplastic in recent years.Just because of the important physiological function that creatine kinase has, more cause noting widely and deep research of people.
The common method of current detection creatine kinase isozyme has:
1. radioimmunology (RIA)
In the past, the methods such as CK-MB many employings electrophoresis, chromatography and immunodepression measure, and afterwards, the advantage such as sensitive with it, special and accurate quantification of RIA and ELISA was subject to the common concern of people, and range of application is increasingly extensive.Wherein, Roberts etc. point out that RIA measures CK isodynamic enzyme and improves 10 ~ 100 times than electrophoresis sensitivity, therefore.Contribute to the match of diagnosis subendocardiac muscle stalk, collections miocardial infarction and cardiac muscle expansion especially.RIA method, in order to avoid CK-MM in blood is on the impact of measurement result, adopts CK-BB antiserum to set up CK-MB rapid radioimmunoassay method.Within domestic 1988, pertinent literature is just had to deliver, its principle first test serum is mixed with a certain amount of CK-BB antiserum, hatched, then the CK-MB of 125I mark is added, anti-CK-MB antibody is competed with the CK-MB in test serum, form antigen-antibody complex, this compound is combined to be formed with the donkey anti-rabbit IgG again added and precipitates, centrifugal removing supernatant, measure sedimentary activity, try to achieve the concentration of CK-MB according to typical curve.But because sensitivity and specificity are not high, and there is the danger of radiocontamination, its clinical practice is restricted, and therefore substantially safer by other, easy method replaces.
2. chemiluminescent immunoassay(CLIA) (CLIA)
Beckman Access CK-MB detects and adopts dibit point enzyme to exempt from method detection (" double antibody sandwich method ").Sample, the rat anti-human CK-MB antibody (enzyme conjugates) that marked alkaline phosphatase and bag are added together in reaction tube by the magnetic-particle of large mouse-anti CK-BB antibody, CK-MB in sample and solid phase are reacted at B subunit (be isomers with CK-BB and the CK-MB) antigen-combining site of the CK-BB antibody on magnetic-particle surface, specific combine with the CK-MB in serum or blood plasma (not with CK-BB and CK-MB isomerism precursor reactant) of enzyme conjugates simultaneously.After hatching, reaction tube is sent to magnetic resolution region and repeatedly rinses, other composition that place to go does not combine with solid phase.Finally in reaction tube, add chemical luminous substrate (Lumi-Phos*530), the alkaline phosphatase be combined with solid phase this substrate can be made to send photon and detect by photolometer.Finally, the light quantum described in multiple spot calibration curve that stores and the corresponding relation of standard items CK-MB in contrast instrument and the CK-MB concentration that calculates in sample, the photon of reaction generation is directly proportional to the content of CK-MB in sample.In the range of linearity, sample can by accurate quantitative analysis (general 0.3 to 300ng/mL).
Roche fully automatic electric chemical illumination immunity analysis instrument principle of work: in human serum, serum/plasma creatine kinase isozyme and its corresponding antibodies (the anti-creatine kinase isozyme monoclonal antibody that biotin labeling and tris (bipyridine) ruthenium mark) meet in reaction tank, forms double antibody compound.After adding the particulate of streptomysin bag quilt, this antibody complex is combined with particulate.After being input to flow cell, due to the effect of magnetic, the materials such as particulate is captured to electrode surface, unconjugated antibody are washed away.Chemiluminescence agent tris (bipyridine) ruthenium and electron donor tripropyl amine (TPA) (TPA), in the reaction of electrode surface generation electrochemiluminescence, by measuring the light intensity sent, converse the concentration of antigen in sample to be measured.Sensing range is between 0.1 ~ 500ng/mL.
3. enzyme linked immunosorbent assay (ELISA)
ELISA is the abbreviation of Enzyme-linked immunosorbent assay.It is a kind of immunoenzyme technics grown up after immunofluorescence and radioimmunoassay technique.Technique is since the beginning of the seventies comes out, and development is very rapid, has been widely used in biology and medical science applied many fields at present.It is double antibody sandwich method that the ELISA of CK-MB measures what adopt, its principle of work uses people's CK-MB (CK-MB) the antibody bag of purifying by microwell plate, make insolubilized antibody, in the micropore that Sheet is anti-, add people's CK-MB (CK-MB) antibody that people's CK-MB (CK-MB) marks with HRP be more successively combined, form antibody-antigene-hrp-antibody complex, after thoroughly washing, add substrate TMB develop the color.TMB changes into blueness under the catalysis of HRP enzyme, and changes into final yellow under the action of an acid.People's CK-MB (CK-MB) in the depth of color and sample is proportionate.Under 450nm wavelength, absorbance (OD value) is measured, by people's CK-MB (CK-MB) concentration in typical curve calculation sample by microplate reader.ELISA method detects one of the most frequently used method of CK-MB at present, and method is simple to operate, and sensitivity is moderate, and instrument price is not high, had a lot of ELISA kit product detecting CK-MB both at home and abroad.
4. fluoroimmunoassay technology (FEIA)
The Triage Meter Plus fluorescence immunity analyzer that U.S. Biosite produces is made up of three parts: test board, Triage measuring appliance and Trige data management software.Each test board principle of work: the experiment mechanism of the advanced person be made up of micropore chromatography and immunofluorescence technique.There is different immune responses at the functional area that test board is different, after sample adds well, filter out haemocyte and other particles.Conversion zone contain fluorescence labeling correspondence reaction antibody or antigen, they can and test substance and mark particle combination.When the sample (experimentally requirement, whole blood, serum or urine) of patient flows through test board, will reaction be produced with the antibody of correspondence and develop the color.Machine operation principle: the measuring system of machine uses laser scanning label and determinand land, obtains fluorescence signal.Then measure fluorescence signal, quantitative analyzes target determinand.Within whole incubation period, the sample of patient constantly will flow through test board.No matter whether put into test section, sample is once inject reaction beginning.Test macro monitor test plate, calculates reaction by the region of read test plate end.When systems axiol-ogy to complete and blood sample all fully covers to hatching, its can the Analysis quality control district of system and fluorescence of reaction zone.The signal of each test section of measurement System Analysis.Judge whether result to be measured can show report.Synchronous reaction Quality Control ensure that test board reaction is normal, and operating process correctly completes.Result can show at LCD, also out printable.
5. gold-marking immunity method
Current gold-marking immunity method is mainly applied in the golden immunochromatographic method (GCIA) combined with immunochromatography technique, and this method sample consumption is few, fast easy, is suitable for the other detection of bed of AMI.Domestic and international product is in this respect relatively many, external Princeton Biomedicines, Inc., Spectral Diagnostics company, Cortez company, Bioseed Diagnostics company, domestic Shenzhen Rui Lai biotech firm, Beijing Ku Er scientific & technical corporation, positive element Sheng Bang biotech firm, Hangzhou Ai Bo biomedical company etc. are all proposed the CK-MB test card utilizing gold-marking immunity ratio juris to prepare, its ultimate principle all: detect free CK-MB with the single combination of two monoclonal anti CK-MB antibody.When sample drips in sucking, first anti-CK-MB antibody/collaurum with CK-MB in conjunction with formation antibody/antigen combination.Second immobilized anti-this combination of CK-MB antibody capture, produces a peach color belt, occurs just not having colour band in reacting ring relative to without combination.Current common prepared by CK-MB, cTnI and MYO tri-kinds of heart stalk marks obstructs three-in-one card intentionally, detect three indexs in heart stalk patient blood, thus more the heart of Accurate Diagnosis patient obstructs situation simultaneously.
Biotin-avidin system (biotinavidin system, BAS) is a kind of new bio reaction amplification system.Along with the appearance of various biotin derivative, BAS is widely used in each field of medical science very soon.This system is applied to SABC, enzyme linked immunological, fluorescence immunoassay, in the detection techniques such as radio-immunity, the susceptibility of above technical method, specificity and stability can be improved significantly, make method easier, contribute to clinical quick diagnosis, and be beneficial to and carry out extensive epidemiology survey, become the immunoreactive powerful of research.Because BAS detection system economy is quick, pollute without radiating matter again, do not need complex instrument, fully show the great potential of this system and the possibility of application.
Biotin-avidin system Cleaning Principle: BAS is on the basis of conventional ELISA principle, in conjunction with the height amplification between biotin and Avidin, and a kind of detection system set up.Avidin is a kind of alkaline glycoprotein extracted in ovalbumin, and molecular weight is 68kDa, is made up of 4 subunits, have very high affinity (binding constant is up to 1015M-1) to biology.Biotin is very easily combined with covalent bond with protein (as antibody etc.).Like this, the Avidin molecule combining enzyme and the biotin molecule being combined with specific antibody produce and react, both served multistage amplification, the colour generation due to the catalytic action of enzyme when running into corresponding substrate, reaches the object detecting unknown antigen (or antibody) molecule again.Streptavidin is and the affine a kind of protein have similar biological properties, in Streptavidin molecule the same as Avidin, every bar peptide chain can in conjunction with a biotin, because nearly all material for marking all can combine with Avidin or streptavidin.Utilize biotin-avidin system to develop creatine kinase isozyme detection examination fast diagnosis reagent and there is no report.
Utility model content
The purpose of this utility model, be to provide a kind of creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper, it provides high sensitivity creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper based on double antibody sandwich method, fluorescence immunoassay lateral chromatography and biotin-Streptavidin amplification system.When using the utility model, improve detection sensitivity and detect stability, reducing non-specific binding, be not more than 10 minutes detection time, substantially increase diagnosis efficiency.
The solution that the utility model solves its technical matters is: creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper, it comprises base plate, described base plate is provided with sample pad successively, fluorescent marker pad, nitrocellulose filter and adsorptive pads, described adsorptive pads and fluorescent marker pad are overlapping to be respectively pressed in behind the two ends of nitrocellulose filter in the formation detection zone, surface of nitrocellulose filter, described sample pad is overlapping to be pressed on fluorescent marker pad, described fluorescent marker pad is fixed with the fluorescin of biotin labeled creatine kinase isozyme monoclonal antibody and marked by streptavidin, nitrocellulose filter in described detection zone is fixed with the nature controlling line of detection line and the sheep anti-mouse igg polyclonal antibody formation identifying that the monoclonal antibody of creatine kinase isozyme another one epi-position is formed.
As the further improvement of technique scheme, described fluorescent marker pad comprises the first stacked fluorescent marker pad and the second fluorescent marker pad, one end pad of described first fluorescent marker pad in the below of sample pad, described second overlapping one end being pressed in nitrocellulose filter of fluorescent marker pad.
As the further improvement of technique scheme, in described first fluorescent marker pad, the one end of inserting below sample pad is provided with positioning strip, and the lower surface of described sample pad is provided with the locating slot that can hold positioning strip.
As the further improvement of technique scheme, also comprise and getting stuck, in described base plate, sample pad, fluorescent marker pad, nitrocellulose filter and adsorptive pads are all placed in and get stuck, the position described shell face of getting stuck corresponding to nitrocellulose membrane is provided with view window, and position shell face of getting stuck corresponding to sample pad is provided with well.
As the further improvement of technique scheme, described view window is slot.
As the further improvement of technique scheme, described fluorescin can be the one in green fluorescent protein, phycobniliprotein etc.
As the further improvement of technique scheme, described sample pad is glass fibre component dry after surfactant damping fluid immersion treatment.
As the further improvement of technique scheme, described base plate is polystyrene component or tygon component.
As the further improvement of technique scheme, described in get stuck and comprise plastics upper casing and plastics lower casing, described plastics upper casing fastens to be formed after on plastics lower casing and gets stuck.
Compared with prior art, tool has the following advantages the utility model:
(1) the utility model is using fluorescin as label, and this label has good stability, and is conducive to improving detecting stability.
(2) the utility model is by utilizing " Streptavidin-biotin amplification system ", improves detection sensitivity, reduces non-specific binding, is conducive to improving kit performance.
(3) detection time utilizing the utility model to carry out Procalcitonin is not more than 10 minutes, and the detection range of linearity is 0.10ng/ml ~ 100.00ng/ml, drastically increases detection efficiency.
(4) the utility model carries out interpretation by fluorescence immunity analyzer to result, can realize robotization, reduces the impact of subjective factor, provides convenient, quick, reliable diagnostic result.
(5) novel the getting stuck of this use is provided with the well that sample enters and the view window supplying observed result, according to analytical instrument result of determination, accurately and reliably.
(6) the utility model is easy to make, and volume is little, be easy to carry.
(7) the utility model testing cost is lower.
(8) the utility model can be mass, and is applicable to clinical quick diagnosis and on-the-spot quick diagnosis; Be easy to preserve, be conducive to grass-roots unit and promote.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the utility model embodiment, below the accompanying drawing used required in describing embodiment is briefly described.Obviously, described accompanying drawing is a part of embodiment of the present utility model, instead of whole embodiment, and those skilled in the art, under the prerequisite not paying creative work, can also obtain other design proposals and accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of the utility model embodiment one;
Fig. 2 is the structural representation of the utility model embodiment two;
Fig. 3 is the structural representation of the utility model embodiment three;
Fig. 4 is the structural representation of the utility model embodiment four.
Embodiment
Be clearly and completely described below with reference to embodiment and the accompanying drawing technique effect to design of the present utility model, concrete structure and generation, to understand the purpose of this utility model, characteristic sum effect fully.Obviously; described embodiment is a part of embodiment of the present utility model, instead of whole embodiment, based on embodiment of the present utility model; other embodiments that those skilled in the art obtains under the prerequisite not paying creative work, all belong to the scope of the utility model protection.In addition, all connection/annexations mentioned in literary composition, not singly refer to that component directly connects, and refer to and according to concrete performance, can connect auxiliary by adding or reducing, and form more excellent draw bail.
With reference to Fig. 1, creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper, it comprises base plate 5, described base plate 5 is provided with sample pad 1 successively, fluorescent marker pad 2, nitrocellulose filter 3 and adsorptive pads 4, described adsorptive pads 4 and fluorescent marker pad 2 are overlapping to be respectively pressed in behind the two ends of nitrocellulose filter 3 in the formation detection zone, surface of nitrocellulose filter 3, fluorescent marker pad 2 is glass fibre membrane, described sample pad 1 is overlapping to be pressed on fluorescent marker pad 2, the fluorescin described fluorescent marker pad 2 being fixed with biotin labeled creatine kinase isozyme monoclonal antibody (concentration 0.3 ~ 1.5mg/mL) and marked by streptavidin (or Avidin) (uses excitation wavelength 530nm, wavelength of transmitted light 570nm, concentration 0.1 ~ 1.0mg/mL), nitrocellulose filter 3 in described detection zone is fixed with the nature controlling line C (concentration 0.2 ~ 2.0mg/mL) of detection line T (concentration 0.5 ~ 3mg/mL) and the sheep anti-mouse igg polyclonal antibody formation identifying that the monoclonal antibody of creatine kinase isozyme another one epi-position is formed, nature controlling line C is used for the validity of test strip.
The fluorescin that biotin labeled creatine kinase isozyme monoclonal antibody and Streptavidin (or Avidin) mark is fixed on fluorescent marker pad 2, the monoclonal antibody and sheep anti-mouse igg polyclonal antibody that have identification creatine kinase isozyme another one epi-position are fixed on nitrocellulose filter as detection line T and nature controlling line C.When testing sample is added to after in sample pad 1, moved forward by chromatography effect, in sample, on creatine kinase isozyme and fluorescent marker pad 2, the creatine kinase isozyme antibody (Mab-CK-MB*Fluoro) of combined with fluorescent label (fluorescin) reacts and forms compound CK-MB-Mab-CK-MB*Fluoro, compound is reacted when continuing to be advanced past CK-MB antibody (detection line) nitrocellulose membrane wrapping quilt under chromatography effect, CK-MB antibody capture formation compound (Mab-CK-MB-CK-MB-Mab-CK-MB*Fluoro) (detection line) that reaction compound is coated, the reaction signal of detection line is read by fluorescence immunity analyzer, under the effect of excitation source, fluorescent material launches the fluorescence signal of specific wavelength, fluorescence immunity analyzer captures fluorescence signal, the typical curve transformed by signal and set is converted into quantitative value automatically, calculate the concentration of creatine kinase isozyme in sample, obtain creatine kinase isozyme testing result.
As the further improvement of technique scheme, with reference to Fig. 3, described fluorescent marker pad 2 comprises the first stacked fluorescent marker pad 20 and the second fluorescent marker pad 21, one end pad of described first fluorescent marker pad 20 in the below of sample pad 1, described second overlapping one end being pressed in nitrocellulose filter 3 of fluorescent marker pad 21.The layering of fluorescent marker pad 2 is arranged, and is convenient to fluorescent marker pad 2 to be arranged between sample pad 1 and nitrocellulose filter 3.
As the further improvement of technique scheme, be provided with positioning strip 22 with reference to one end of inserting below sample pad in the first fluorescent marker pad 20 described in Fig. 3, the lower surface of described sample pad 1 is provided with the locating slot that can hold positioning strip 22.The installation be convenient between fluorescent marker pad 2 and sample pad 1 by positioning strip 22 is fixed.
As the further improvement of technique scheme, with reference to Fig. 2 and Fig. 4, also comprise and getting stuck, described base plate 5, sample pad 1, fluorescent marker pad 2, nitrocellulose filter 3 and adsorptive pads 4 are all placed in and get stuck in 6, the position described 6 shell faces of getting stuck corresponding to nitrocellulose membrane 3 is provided with view window 8, and the position 6 shell faces of getting stuck corresponding to sample pad 1 is provided with well 7.
As the further improvement of technique scheme, described view window 8 is slot.
As the further improvement of technique scheme, described fluorescin can be the one in green fluorescent protein, phycobniliprotein etc.
As the further improvement of technique scheme, described sample pad 1 is glass fibre component dry after surfactant damping fluid immersion treatment.Sample pad is made up of glass fibre, through surfactant damping fluid immersion treatment, uses after dry.The cutting width of described sample pad 1 is 0.3 ~ 0.5cm
As the further improvement of technique scheme, described base plate 5 is polystyrene component or tygon component.
As the further improvement of technique scheme, described in get stuck and 6 comprise plastics upper casing 60 and plastics lower casing 61, described plastics upper casing 61 fastens to be formed after on plastics lower casing 61 and gets stuck 6.
More than that better embodiment of the present utility model is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modifications or replacement under the prerequisite without prejudice to the utility model spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (9)
1. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper, it is characterized in that: it comprises base plate, described base plate is provided with sample pad successively, fluorescent marker pad, nitrocellulose filter and adsorptive pads, described adsorptive pads and fluorescent marker pad are overlapping to be respectively pressed in behind the two ends of nitrocellulose filter in the formation detection zone, surface of nitrocellulose filter, described sample pad is overlapping to be pressed on fluorescent marker pad, described fluorescent marker pad is fixed with the fluorescin of biotin labeled creatine kinase isozyme monoclonal antibody and marked by streptavidin, nitrocellulose filter in described detection zone is fixed with the nature controlling line of detection line and the sheep anti-mouse igg polyclonal antibody formation identifying that the monoclonal antibody of creatine kinase isozyme another one epi-position is formed.
2. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 1, it is characterized in that: described fluorescent marker pad comprises the first stacked fluorescent marker pad and the second fluorescent marker pad, one end pad of described first fluorescent marker pad in the below of sample pad, described second overlapping one end being pressed in nitrocellulose filter of fluorescent marker pad.
3. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 2, it is characterized in that: in described first fluorescent marker pad, the one end of inserting below sample pad is provided with positioning strip, and the lower surface of described sample pad is provided with the locating slot that can hold positioning strip.
4. the creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to any one of claims 1 to 3, it is characterized in that: also comprise and getting stuck, in described base plate, sample pad, fluorescent marker pad, nitrocellulose filter and adsorptive pads are all placed in and get stuck, the position described shell face of getting stuck corresponding to nitrocellulose membrane is provided with view window, and position shell face of getting stuck corresponding to sample pad is provided with well.
5. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described view window is slot.
6. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described fluorescin can be the one in green fluorescent protein, phycobniliprotein etc.
7. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described sample pad is glass fibre component dry after surfactant damping fluid immersion treatment.
8. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described base plate is polystyrene component or tygon component.
9. creatine kinase isozyme immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described in get stuck and comprise plastics upper casing and plastics lower casing, described plastics upper casing fastens to be formed after on plastics lower casing and gets stuck.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105527445A (en) * | 2015-12-30 | 2016-04-27 | 天津诺星生物医药科技有限公司 | Acute myocardial infarction detection system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105527445A (en) * | 2015-12-30 | 2016-04-27 | 天津诺星生物医药科技有限公司 | Acute myocardial infarction detection system |
| CN105527445B (en) * | 2015-12-30 | 2018-04-17 | 天津诺星生物医药科技有限公司 | A kind of acute myocardial infarction detecting system |
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