CN204185494U - For the test kit of the placenta stem-cell that dissociates fast, purifies - Google Patents
For the test kit of the placenta stem-cell that dissociates fast, purifies Download PDFInfo
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- CN204185494U CN204185494U CN201420503371.7U CN201420503371U CN204185494U CN 204185494 U CN204185494 U CN 204185494U CN 201420503371 U CN201420503371 U CN 201420503371U CN 204185494 U CN204185494 U CN 204185494U
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Abstract
The utility model discloses a kind of test kit for the placenta stem-cell that dissociates fast, purifies, it comprises the test kit that inside is provided with screens groove, described test kit comprise hold respectively placenta dissociate the placenta of reagent dissociate reagent bottle, hold the stem cell purification reagent bottle of stem cell purification reagent, the described placenta reagent that dissociates is collagenase, and stem cell purification reagent is hydroxyethylamyle.This test kit is practical and convenient, is applicable to dissociate fast, extract placenta stem-cell, eliminates the tedious steps preparing aseptic reagent, avoids polluting.
Description
Technical field
The utility model belongs to test kit, is specifically related to a kind of test kit for the placenta stem-cell that dissociates fast, purifies.
Background technology
Placenta stem-cell refers to the stem cell be present in placenta tissue, comprises hemopoietic stem cell, mescenchymal stem cell etc.Hemopoietic stem cell is the founder of red corpuscle, white corpuscle, thrombocyte etc., and being highly undifferentiated cell, is the initiating cell of all hemocytes and immunocyte.Hematopoietic stem cell transplantation can treat malignant hematologic disease, part malignant tumour, tens kinds of fatal diseases such as part heredopathia.Placenta tissue hemopoietic stem cell quantity is enriched, and is 5 ~ 10 times of Cord blood, breaches the bottleneck that Cord Blood-Derived hemopoietic stem cell 30kg body weight uses, stem cell population can be provided enough to rebuild the hemopoietic system of grownup.
Extensively carry out the clinical application research of placenta stem-cell at present, the disease that treatment is relevant comprises: the pernicious or non-malignant hematologic diseases such as aplastic anaemia, myelodysplastic syndrome, leukemia, and the non-blood systemic disease such as diabetes B, idiopathic pulmonary fibrosis, ankylosing spondylitis, ulcerative colitis.
Placenta stem-cell has wide potential applicability in clinical practice.But, be separated placenta stem-cell many employings traditional-handwork configuration all ingredients at present, there is no the simple, fast and efficient test kit dissociating, extract placenta stem-cell.
Summary of the invention
In order to overcome the problems referred to above that prior art field exists, the purpose of this utility model is, a kind of test kit of placenta stem-cell for rodent of dissociating fast, purify, the mankind or other Mammalss source is provided, have simple and quick, easy to use, easy to operate, can suitability for industrialized production, practicability and effectiveness advantage, application prospect is extensive.
The test kit for the placenta stem-cell that dissociates fast, purifies that the utility model provides, it comprises the test kit that inside is provided with screens groove, described test kit comprise hold respectively placenta dissociate the placenta of reagent dissociate reagent bottle, hold the stem cell purification reagent bottle of stem cell purification reagent, the described placenta reagent that dissociates is collagenase, and stem cell purification reagent is hydroxyethylamyle.
Described test kit also comprises the placenta pretreating reagent bottle holding placenta pretreating reagent, and described placenta pretreating reagent is containing dual anti-PBS or physiological saline.
The described placenta placenta dissociated in the reagent bottle reagent that dissociates is any one in NTx enzyme or type Ⅳ collagenase.
Described placenta agent treated time of dissociating is 30-45min.
The described stem cell purification agent treated time is for being less than or equal to 15min.
Described test kit also comprises the stem cell washing reagent bottle holding stem cell washing composition, and described stem cell washing composition is PBS or physiological saline.
The test kit for the placenta stem-cell that dissociates fast, purifies that the utility model provides, its beneficial effect is, this test kit is practical and convenient, is applicable to dissociate fast, extract placenta stem-cell, eliminates the tedious steps preparing aseptic reagent, avoids polluting.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of the utility model embodiment.
Mark in figure:
1. test kit; 2. placenta pretreating reagent bottle; 3. placenta dissociates reagent bottle; 4. stem cell purification reagent bottle; 5. stem cell washing reagent bottle.
Embodiment
With reference to the accompanying drawings, in conjunction with the embodiments, to the test kit for the placenta stem-cell that dissociates fast, purifies that the utility model provides, be described in detail.
Embodiment
With reference to Fig. 1, the test kit for the placenta stem-cell that dissociates fast, purifies of the present embodiment, it comprises the test kit 1 that inside is provided with screens groove, described test kit 1 comprise hold respectively placenta dissociate the placenta of reagent dissociate reagent bottle 3, hold the stem cell purification reagent bottle 5 of stem cell purification reagent, the described placenta reagent that dissociates is collagenase, and stem cell purification reagent is hydroxyethylamyle.
Described test kit 1 also comprises placenta pretreating reagent, is contained in placenta pretreating reagent bottle 2, and placenta pretreating reagent is containing dual anti-PBS.
The collagenase that the placenta of described test kit 1 dissociates in reagent bottle 3 is the NTx enzyme of concentration 1mg/ml.
Described test kit 1 also comprises stem cell washing composition, is PBS, is contained in stem cell washing reagent bottle 5.
The screens groove of described placement reagent bottle by porous plastics, cardboard or other make.
The closing cap of described reagent bottle is chosen as pressure or screw.
Described placenta agent treated time of dissociating is 30min, and temperature is 37 DEG C.
The described stem cell purification agent treated time is 10min.
The object that described test kit can act on is rodent, people or other mammiferous placentas.
Also comprise working instructions, for illustration of the using method of stem cell of dissociating fast from placenta, purify.
Wherein, whole operating process remains in aseptic Working environment and operates, and wherein, intracellular toxin detection level≤0.5EU/mL represents that test kit is qualified.
Test kit of the present utility model uses and comprises the steps:
A washs, shreds placenta: the placenta pretreating reagent bottle 2 adding appropriate volume includes dual anti-PBS vibration washing placenta 2 ~ 3 times, to the bloodstain washes clean on placenta surface.Remove amnion and the decidua on placenta surface, add the placenta pretreating reagent of appropriate volume, placenta tissue is shredded, and the blood in tissue block is cleaned up, collect placenta pretreating reagent, in the centrifugal 10min of 1800rpm room temperature, abandon supernatant, by 50ml stem cell washing composition re-suspended cell precipitation, add stem cell purification reagent in the ratio of 4:1, in the centrifugal 10min of 300rpm room temperature after mixing, collect supernatant liquid.
B dissociates placenta: collect the above-mentioned placenta tissue block shredded, add the reagent that to dissociate with the isopyknic placenta of placenta tissue block, 30 ~ 45min is digested in 37 DEG C of shaking baths, after digesting, placenta tissue block is through 200 order analysis sieve collecting by filtration filtrates, in the centrifugal 10min of 1800rpm room temperature after mixing, abandons supernatant, precipitate with appropriate stem cell washing composition re-suspended cell, the same ratio in 4:1 adds stem cell purification reagent, in the centrifugal 10min of 300rpm room temperature after mixing, collects supernatant liquid.
C harvested cell: the supernatant liquid collected in combining step a and b, centrifugal supernatant of abandoning obtains placenta stem-cell.
Embodiment two
Of the present utility model for dissociating, purifying placenta stem-cell test kit fast, test kit 1 comprises placenta and to dissociate reagent, stem cell purification reagent, being contained in placenta respectively dissociates in reagent bottle 3, stem cell purification reagent bottle 4, the placenta reagent comprised in reagent bottle 3 that dissociates is collagenase, and the reagent comprised in stem cell purification reagent bottle 4 is hydroxyethylamyle.
Wherein said test kit 1 also comprises placenta pretreating reagent, is contained in placenta pretreating reagent bottle 2, for containing dual anti-PBS.
The collagenase that the placenta of wherein said test kit 1 dissociates in reagent bottle 3 is the type Ⅳ collagenase of concentration 1mg/ml.
Wherein said test kit 1 also comprises stem cell washing composition, is physiological saline, is put in stem cell washing reagent bottle 5.
The screens groove of described placement reagent bottle by porous plastics, cardboard or other make.
The closing cap of described reagent bottle is chosen as pressure or screw.
Described placenta agent treated time of dissociating is 45min, and temperature is 37 DEG C.
The described stem cell purification agent treated time is 10min.
The object that described test kit can act on is rodent, people or other mammiferous placentas.
Also comprise working instructions, for illustration of the using method of stem cell of dissociating fast from placenta, purify.
Wherein, whole operating process remains in aseptic Working environment and operates, and wherein, intracellular toxin detection level≤0.5EU/mL represents that test kit is qualified.
The utility model is the explanation carrying out test kit with above-described embodiment, is not meant to limit the use of test kit, and any equivalent replacement carried out this, amendment, increase all drop in test kit of the present utility model protection category.
Claims (2)
1. the test kit for the placenta stem-cell that dissociates fast, purifies, it comprises the test kit that inside is provided with screens groove, it is characterized in that: described test kit comprise hold respectively placenta dissociate the placenta of reagent dissociate reagent bottle, hold the stem cell purification reagent bottle of stem cell purification reagent, the described placenta reagent that dissociates is collagenase, and stem cell purification reagent is hydroxyethylamyle.
2. the test kit for the placenta stem-cell that dissociates fast, purifies according to claim 1, it is characterized in that: described test kit also comprises the placenta pretreating reagent bottle holding placenta pretreating reagent, described placenta pretreating reagent is containing dual anti-PBS or physiological saline.
3. the test kit for the placenta stem-cell that dissociates fast, purifies according to claim 1, is characterized in that: the described placenta placenta dissociated in the reagent bottle reagent that dissociates is any one in NTx enzyme or type Ⅳ collagenase.
4. the test kit for the placenta stem-cell that dissociates fast, purifies according to claim 1, is characterized in that: described test kit also comprises the stem cell washing reagent bottle holding stem cell washing composition, and described stem cell washing composition is PBS or physiological saline.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420503371.7U CN204185494U (en) | 2014-09-03 | 2014-09-03 | For the test kit of the placenta stem-cell that dissociates fast, purifies |
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| CN201420503371.7U CN204185494U (en) | 2014-09-03 | 2014-09-03 | For the test kit of the placenta stem-cell that dissociates fast, purifies |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969721A (en) * | 2016-07-19 | 2016-09-28 | 安徽惠恩生物科技股份有限公司 | Extraction preparation method of placenta hematopoietic stem cells |
| CN109896157A (en) * | 2019-03-13 | 2019-06-18 | 广州出入境检验检疫局综合技术服务中心 | A kind of stem cell kit and its application method with purification function |
| CN112755177A (en) * | 2021-03-15 | 2021-05-07 | 浙江大学 | Oral liquid containing chick embryo EGF, preparation method and application |
-
2014
- 2014-09-03 CN CN201420503371.7U patent/CN204185494U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969721A (en) * | 2016-07-19 | 2016-09-28 | 安徽惠恩生物科技股份有限公司 | Extraction preparation method of placenta hematopoietic stem cells |
| CN109896157A (en) * | 2019-03-13 | 2019-06-18 | 广州出入境检验检疫局综合技术服务中心 | A kind of stem cell kit and its application method with purification function |
| CN109896157B (en) * | 2019-03-13 | 2021-06-15 | 广州出入境检验检疫局综合技术服务中心 | Stem cell kit with purification function and use method thereof |
| CN112755177A (en) * | 2021-03-15 | 2021-05-07 | 浙江大学 | Oral liquid containing chick embryo EGF, preparation method and application |
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Effective date of registration: 20160805 Address after: 266000, No. 1, 2, building 7, Qingdao blue bio Pharmaceutical Industrial Park, 368 Hedong Road, Hedong District, Qingdao, Shandong Patentee after: QINGDAO RE-STORE BIOTECH CO., LTD. Address before: 100000 Beijing city Chaoyang District cuigezhuang maquanying No. 20 floor two room 288 Patentee before: BEIJING RUISIDE BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. |