CN109896157A - A kind of stem cell kit and its application method with purification function - Google Patents

A kind of stem cell kit and its application method with purification function Download PDF

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Publication number
CN109896157A
CN109896157A CN201910187223.6A CN201910187223A CN109896157A CN 109896157 A CN109896157 A CN 109896157A CN 201910187223 A CN201910187223 A CN 201910187223A CN 109896157 A CN109896157 A CN 109896157A
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CN
China
Prior art keywords
hole
box
stem cell
groups
reagent bottle
Prior art date
Application number
CN201910187223.6A
Other languages
Chinese (zh)
Inventor
申国辉
都兴洋
叶丹
刘亚州
Original Assignee
广州出入境检验检疫局综合技术服务中心
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by 广州出入境检验检疫局综合技术服务中心 filed Critical 广州出入境检验检疫局综合技术服务中心
Priority to CN201910187223.6A priority Critical patent/CN109896157A/en
Publication of CN109896157A publication Critical patent/CN109896157A/en

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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics

Abstract

The present invention provides a kind of stem cell kit with purification function, including box body and box cover, the box cover left end is mounted on cassette upper end left side of face edge by hinge, four groups of foam strips are installed side by side in the box body, the foam strip upper surface successively opens up through-hole one from left to right, through-hole two and through-hole three, the fixed square block of the tray interior front end face, press strip is arranged in the foam strip upper end, the box body lower end surface opens up blind hole, straight thread cylinder is fixed in box cover inner upper end face, threaded rod is engaged by screw thread in the straight thread cylinder, the box cover upper surface opens up circular hole, compared with prior art, the present invention is with following the utility model has the advantages that achieving the purpose that local replacing by installing multiple groups foam strip side by side in box body, by being assigned after back-outing the threaded rod on one group of box cover out of straight thread cylinder to another group of box body On blind hole in reach reduce two adjacent groups kit between contact area.

Description

A kind of stem cell kit and its application method with purification function
Technical field
The present invention is a kind of stem cell kit and its application method with purification function, belongs to field of biotechnology.
Background technique
Stem cell is a kind of multipotential cell with the of self-replication capacity.Under certain condition, it can be divided into more Kind functioning cell.The stage of development according to locating for stem cell is divided into embryonic stem cell and adult stem cell.According to the hair of stem cell It educates potential to be divided into three classes: myeloid-lymphoid stem cell, multipotential stem cell and unipotent stem cell.Stem cell is a kind of inabundant differentiation, still not Mature cell, has the potential function for regenerating various histoorgans and human body, and medical field is known as " general-purpose cell ".
Reagent type for purifying stem cell is more, places for specification, usually various reagents are placed in kit It being saved, some reagents need low temperature, and when existing kit is stacked in low temperature environment, because kit connects between each other Contacting surface product is larger, and the probability to stick together in low temperature environment is larger, is unfavorable for the taking-up in later period, is anti-reagent bottle in kit Interior shaking, often assigns into reagent bottle in foam, and reagent bottle assigns the hole on rear foam easy to damage because rubbing repeatedly, loses to examination The clamping effect of agent bottle, and whole discarded relatively waste.
Summary of the invention
In view of the deficienciess of the prior art, it is an object of the present invention to provide a kind of stem cell kits with purification function And its application method, to solve the problems mentioned in the above background technology, the present invention can local replacing, have low-temp storage it is antifreeze Junction structure, it is easy to use.
To achieve the goals above, the present invention is to realize by the following technical solutions: a kind of to have the function of purification Stem cell kit, including box body, box cover, normal saline bottle, hydroxyethyl starch reagent bottle and clostridiopetidase A reagent bottle, the box Lid left end is mounted on cassette upper end left side of face edge by hinge, installs four groups of foam strips, the bubble in the box body side by side Foam upper surface successively opens up through-hole one, through-hole two and through-hole three from left to right, and the normal saline bottle assigns into through-hole one Interior, the hydroxyethyl starch reagent bottle assigns into through-hole two, and the clostridiopetidase A reagent bottle assigns into through-hole three, the box body Square block is fixed in interior forward end face, and press strip is arranged in the foam strip upper end, and the press strip front end is fixed by bolt and square block It connects and press strip is arranged on the downside of square block, the box body lower end surface opens up blind hole, the fixed straight spiral shell in box cover inner upper end face Line cylinder, the straight thread cylinder is interior to engage threaded rod by screw thread, and the box cover upper surface opens up circular hole, and the circular hole is arranged straight On the upside of thread barrel.
Further, the threaded rod, circular hole, straight thread cylinder and blind hole are provided with four groups, blind hole described in four groups point It is not provided with the edges and corners of box body lower end surface four, circular hole described in four groups is provided with the edges and corners of box cover upper surface four respectively, and four groups The straight thread cylinder is separately fixed at four, box cover inner upper end face edges and corners, threaded rod described in four groups be engaged on respectively four groups it is straight In thread barrel.
Further, the threaded rod upper end processes tip.
Further, the central axes of the circular hole coincide with blind hole central axes.
It is a kind of with purification function stem cell kit application method, comprising the following steps: foam strip upper surface from Left-to-right successively opens up through-hole one, through-hole two and through-hole three, and through-hole one, through-hole two and through-hole three realize physiological saline respectively Bottle, hydroxyethyl starch reagent bottle and clostridiopetidase A reagent bottle are assigned, and normal saline bottle assigns into through-hole one, normal saline bottle In physiological saline be used for the washing of stem cell, hydroxyethyl starch reagent bottle assigns into through-hole two, hydroxyethyl starch reagent bottle Interior hydroxyethyl starch reagent is used for the purification of stem cell, and clostridiopetidase A reagent bottle assigns into through-hole three, in clostridiopetidase A reagent bottle Collagen enzymatic reagent be used for placenta dissociation.
Beneficial effects of the present invention: a kind of stem cell kit with purification function of the invention, because the present invention adds Foam strip, press strip, square block, through-hole one, through-hole two and through-hole three, the design are steeped by installing in box body multiple groups side by side Foam item achievees the purpose that local replacing, carries out pressure holding to foam strip using press strip and square block and reaches foam-resistant voluntary activity Purpose avoids the foam strip when extracting normal saline bottle, hydroxyethyl starch reagent bottle or clostridiopetidase A reagent bottle out from being pulled up together.
Because the present invention is added to threaded rod, circular hole, straight thread cylinder and blind hole, the design is by by the spiral shell on one group of box cover Rasp bar, which is assigned after back-outing out of straight thread cylinder to reaching in the blind hole on another group of box body, to be reduced between two adjacent groups kit Contact area, reduce in low temperature environment storage occur stick to each other probability, the present invention can local replacing, have low-temp storage Antifreeze junction structure, it is easy to use.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention, Objects and advantages will become more apparent upon:
Fig. 1 is a kind of structural schematic diagram of the stem cell kit with purification function of the present invention;
Fig. 2 is the cross-sectional view of box body in a kind of stem cell kit with purification function of the present invention;
Fig. 3 is the cross-sectional view of box cover in a kind of stem cell kit with purification function of the present invention;
Fig. 4 is the bottom view of box body in a kind of stem cell kit with purification function of the present invention;
Fig. 5 is the cross-sectional view of foam strip in a kind of stem cell kit with purification function of the present invention;
In figure: 1- box body, 2- box cover, 3- threaded rod, 4- normal saline bottle, 5- hydroxyethyl starch reagent bottle, 6- collagen enzymatic reagent Bottle, 7- foam strip, 8- press strip, 9- square block, 10- circular hole, 11- straight thread cylinder, 12- blind hole, 13- through-hole one, 14- through-hole two, 15- through-hole three.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to Specific embodiment, the present invention is further explained.
Fig. 1-Fig. 5 is please referred to, the present invention provides a kind of technical solution: a kind of stem cell kit with purification function, Including box body 1, box cover 2, normal saline bottle 4, hydroxyethyl starch reagent bottle 5 and clostridiopetidase A reagent bottle 6,2 left end of box cover passes through Hinge is mounted at 1 upper surface left side edge of box body, installs four groups of foam strips 7 in box body 1 side by side, passes through four groups of foam strips 7 Design achievees the purpose that the local replacing after one group of foam strip 7 damages, and avoids integral replacing, 7 upper surface of foam strip is from left to right Through-hole 1, through-hole 2 14 and through-hole 3 15 are successively opened up, through-hole 1, through-hole 2 14 and through-hole 3 15 realize life respectively Assigning for saline bottle 4, hydroxyethyl starch reagent bottle 5 and clostridiopetidase A reagent bottle 6 is managed, normal saline bottle 4 assigns into through-hole 1 Interior, the physiological saline in normal saline bottle 4 is used for the washing of stem cell, and hydroxyethyl starch reagent bottle 5 assigns into through-hole 2 14, Hydroxyethyl starch reagent in hydroxyethyl starch reagent bottle 5 is used for the purification of stem cell, and clostridiopetidase A reagent bottle 6 assigns into through-hole three In 15, the collagen enzymatic reagent in clostridiopetidase A reagent bottle 6 is used for the dissociation of placenta, the fixed square block 9 in 1 interior forward end face of box body, bubble Press strip 8 is arranged in 7 upper end of foam item, and 8 front end of press strip is fixedly connected by bolt with square block 9 and press strip 8 is arranged under square block 9 Press strip 8 is fixedly connected by side by bolt with square block 9, realizes pressure holding of the press strip 8 to foam strip 7,1 lower end surface of box body opens up Blind hole 12, the fixed straight thread cylinder 11 in 2 inner upper end face of box cover, by screw thread engagement threaded rod 3 in straight thread cylinder 11, on box cover 2 End face opens up circular hole 10, and the setting of circular hole 10 when needing deepfreeze, turns the spiral shell in straight thread cylinder 11 in 11 upside of straight thread cylinder Rasp bar 3 then assigns the threaded rod 3 on one group of box cover 2 until 3 upper end of threaded rod passes through circular hole 10 and extends to 2 upside of box cover In blind hole 12 on to another group of box body 1, and then the contact area between one group of box cover 2 and another group of box body 1 is reduced, then dropped The low probability for mutually freezing adhesion.
Threaded rod 3, circular hole 10, straight thread cylinder 11 and blind hole 12 are provided with four groups, and four groups of blind holes 12 are provided with respectively Box body 1 lower end surface four edges and corners, four groups of circular holes 10 are provided with box cover 2 upper surface four edges and corners, four groups of straight thread cylinders respectively 11 are separately fixed at four, 2 inner upper end face of box cover edges and corners, and four groups of threaded rods 3 are engaged on respectively in four groups of straight thread cylinders 11, The design of four groups of threaded rods 3, circular hole 10, straight thread cylinder 11 and blind hole 12 achievees the purpose that uniform support.
3 upper end of threaded rod processes tip, and 3 upper end of threaded rod processing tip reduction threaded rod 3 connects with 12 inner surface of blind hole Contacting surface product, further decreases the probability for freezing adhesion.
The central axes of circular hole 10 coincide with 12 central axes of blind hole, and central axes and 12 central axes of blind hole of circular hole 10 coincide Convenient for the stacking between two adjacent groups kit.
It is a kind of with purification function stem cell kit application method, comprising the following steps: 7 upper surface of foam strip from Left-to-right successively opens up through-hole 1, through-hole 2 14 and through-hole 3 15, and through-hole 1, through-hole 2 14 and through-hole 3 15 are distinguished Realize assigning for normal saline bottle 4, hydroxyethyl starch reagent bottle 5 and clostridiopetidase A reagent bottle 6, normal saline bottle 4 assigns into logical In hole 1, the physiological saline in normal saline bottle 4 is used for the washing of stem cell, and hydroxyethyl starch reagent bottle 5 assigns into through-hole In 2 14, the hydroxyethyl starch reagent in hydroxyethyl starch reagent bottle 5 is used for the purification of stem cell, and clostridiopetidase A reagent bottle 6 is assigned In through-hole 3 15, the collagen enzymatic reagent in clostridiopetidase A reagent bottle 6 is used for the dissociation of placenta.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, for this field skill For art personnel, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from spirit of the invention or In the case where essential characteristic, the present invention can be realized in other specific forms.Therefore, in all respects, should all incite somebody to action Embodiment regards exemplary as, and is non-limiting, the scope of the present invention by appended claims rather than on state Bright restriction, it is intended that including all changes that fall within the meaning and scope of the equivalent elements of the claims in the present invention It is interior.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art The other embodiments being understood that.

Claims (5)

1. a kind of stem cell kit with purification function, including box body, box cover, normal saline bottle, hydroxyethyl starch reagent Bottle and clostridiopetidase A reagent bottle, it is characterised in that: the box cover left end is mounted on cassette upper end left side of face edge by hinge, Four groups of foam strips are installed side by side in the box body, the foam strip upper surface successively open up from left to right through-hole one, through-hole two with And through-hole three, the normal saline bottle assign into through-hole one, the hydroxyethyl starch reagent bottle assigns into through-hole two, described Clostridiopetidase A reagent bottle assigns into through-hole three, the fixed square block of the tray interior front end face, the foam strip upper end setting pressure Item, the press strip front end is fixedly connected by bolt with square block and press strip is arranged on the downside of square block, the box body lower end surface Blind hole is opened up, the fixed straight thread cylinder in box cover inner upper end face, the straight thread cylinder is interior to engage threaded rod by screw thread, described Box cover upper surface opens up circular hole, and the circular hole is arranged on the upside of straight thread cylinder.
2. a kind of stem cell kit with purification function according to claim 1, it is characterised in that: the screw thread Bar, circular hole, straight thread cylinder and blind hole are provided with four groups, and blind hole described in four groups is provided with the corner angle of box body lower end surface four respectively Place, circular hole described in four groups are provided with the edges and corners of box cover upper surface four respectively, and straight thread cylinder described in four groups is separately fixed at box cover Four, inner upper end face edges and corners, threaded rod described in four groups are engaged on respectively in four groups of straight thread cylinders.
3. a kind of stem cell kit with purification function according to claim 1, it is characterised in that: the threaded rod Upper end processes tip.
4. a kind of stem cell kit with purification function according to claim 1, it is characterised in that: the circular hole Central axes coincide with blind hole central axes.
5. a kind of a kind of application method of the stem cell kit with purification function according to claim 1 to 4, It is characterized by comprising following steps: foam strip upper surface successively opens up through-hole one, through-hole two and through-hole three from left to right, Through-hole one, through-hole two and through-hole three realize normal saline bottle, hydroxyethyl starch reagent bottle and clostridiopetidase A reagent bottle respectively It assigns, normal saline bottle assigns into through-hole one, and the physiological saline in normal saline bottle is used for the washing of stem cell, and ethoxy forms sediment Powder reagent bottle assigns into through-hole two, and the hydroxyethyl starch reagent in hydroxyethyl starch reagent bottle is used for the purification of stem cell, glue Protoenzyme reagent bottle assigns into through-hole three, and the collagen enzymatic reagent in clostridiopetidase A reagent bottle is used for the dissociation of placenta.
CN201910187223.6A 2019-03-13 2019-03-13 A kind of stem cell kit and its application method with purification function CN109896157A (en)

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CN201910187223.6A CN109896157A (en) 2019-03-13 2019-03-13 A kind of stem cell kit and its application method with purification function

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Application Number Priority Date Filing Date Title
CN201910187223.6A CN109896157A (en) 2019-03-13 2019-03-13 A kind of stem cell kit and its application method with purification function

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CN109896157A true CN109896157A (en) 2019-06-18

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Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2045726U (en) * 1989-03-30 1989-10-11 武汉铁路分局武昌东车站 Binding unit for sheet steel
JPH08198333A (en) * 1995-01-20 1996-08-06 Sekisui Plastics Co Ltd Packing cushioning material and packing case with the cushioning material
CN2739982Y (en) * 2004-06-17 2005-11-16 李玉明 Tobacco moisture box with lining pad
CN2931346Y (en) * 2006-06-27 2007-08-08 宝山钢铁股份有限公司 Packing support for oil well pipe
JP2008222263A (en) * 2007-03-12 2008-09-25 Takahisa Watanabe Packaging device
JP3174178U (en) * 2011-12-26 2012-03-08 ▲うぇい▼強科技股▲ふん▼有限公司 Shell package
CN202281450U (en) * 2011-09-07 2012-06-20 慈溪市飞龙电器有限公司 Refrigerator drawer
CN202823420U (en) * 2012-10-08 2013-03-27 重庆博士泰生物技术有限公司 Portable anti-vibration kit
CN203333673U (en) * 2011-10-28 2013-12-11 李福生 Kit for enriching and purifying stem cells
US20150021322A1 (en) * 2013-07-18 2015-01-22 Dean Rosenblum Storage container systems and methods
CN204185494U (en) * 2014-09-03 2015-03-04 北京瑞思德生物科技有限公司 For the test kit of the placenta stem-cell that dissociates fast, purifies
CN105984656A (en) * 2015-01-26 2016-10-05 茂迪股份有限公司 Wafer cassette
CN207472899U (en) * 2017-11-13 2018-06-08 山东朱氏药业集团有限公司 A kind of kit for quantitatively detecting NGAL in urine or blood

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2045726U (en) * 1989-03-30 1989-10-11 武汉铁路分局武昌东车站 Binding unit for sheet steel
JPH08198333A (en) * 1995-01-20 1996-08-06 Sekisui Plastics Co Ltd Packing cushioning material and packing case with the cushioning material
CN2739982Y (en) * 2004-06-17 2005-11-16 李玉明 Tobacco moisture box with lining pad
CN2931346Y (en) * 2006-06-27 2007-08-08 宝山钢铁股份有限公司 Packing support for oil well pipe
JP2008222263A (en) * 2007-03-12 2008-09-25 Takahisa Watanabe Packaging device
CN202281450U (en) * 2011-09-07 2012-06-20 慈溪市飞龙电器有限公司 Refrigerator drawer
CN203333673U (en) * 2011-10-28 2013-12-11 李福生 Kit for enriching and purifying stem cells
JP3174178U (en) * 2011-12-26 2012-03-08 ▲うぇい▼強科技股▲ふん▼有限公司 Shell package
CN202823420U (en) * 2012-10-08 2013-03-27 重庆博士泰生物技术有限公司 Portable anti-vibration kit
US20150021322A1 (en) * 2013-07-18 2015-01-22 Dean Rosenblum Storage container systems and methods
CN204185494U (en) * 2014-09-03 2015-03-04 北京瑞思德生物科技有限公司 For the test kit of the placenta stem-cell that dissociates fast, purifies
CN105984656A (en) * 2015-01-26 2016-10-05 茂迪股份有限公司 Wafer cassette
CN207472899U (en) * 2017-11-13 2018-06-08 山东朱氏药业集团有限公司 A kind of kit for quantitatively detecting NGAL in urine or blood

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