CN204142671U - Electric pole type blood sugar examination bar - Google Patents
Electric pole type blood sugar examination bar Download PDFInfo
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- CN204142671U CN204142671U CN201420555844.8U CN201420555844U CN204142671U CN 204142671 U CN204142671 U CN 204142671U CN 201420555844 U CN201420555844 U CN 201420555844U CN 204142671 U CN204142671 U CN 204142671U
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Abstract
The utility model discloses a kind of electric pole type blood sugar examination bar, comprise substrate layer successively from top to bottom, electrode layer, insulation course, layers of two-sided and hydrophilic membrane, electrode layer is provided with the first hematocrit and compares test electrode, second hematocrit compares test electrode, working electrode, contrast electrode, this hearth electrode, starting electrode and the wire making above-mentioned each electrode and surveying instrument form circuit to be connected, insulation course and layers of two-sided are equipped with and expose above-mentioned first hematocrit and compare test electrode, second hematocrit compares test electrode, working electrode, the breach of contrast electrode and this hearth electrode, hydrophilic membrane covers this breach, the contrast electrode exposed by above-mentioned breach and this hearth electrode are by the upper water color ink containing transmitting electronic media of serigraphy coating, and the working electrode exposed by above-mentioned breach is by the upper water color ink containing transmitting electronic media and FAD-GDH of serigraphy coating.
Description
Technical field
The utility model belongs to blood sugar test technical field, is specifically related to a kind of electric pole type blood sugar examination bar.
Background technology
In order to meet the demand of the level of the daily monitoring glucose in blood of growing diabetic, market there is a large amount of glucose meter tables, wherein most blood glucose meter is all adopt electrochemical method, Momentary electronic is produced by media capture to afterwards during glucose response in enzymatic blood, under the effect of voltage, pass to electrode form current return, blood glucose meter detects this current signal and changes into concentration of glucose and reads value and show.
What most of blood sugar electrode adopted in the market is two electrodes or three electrode method test blood sugar concentration; usually can by the impact of other interfering materials existed in sample in these electrode testing procedure; the impact being also subject to the hematocrit ratio of blood is very large, have impact on the accuracy of test result greatly.In the selection of enzyme, most of blood sugar electrode adopts glucose oxidase, seriously can be subject to the oxygen in air or the impact of blood oxygen dividing potential drop, and when causing finger tip blood and venous blood to be tested, result has deviation.In the selection of medium, most of blood sugar electrode adopts the potassium ferricyanide or Ferrocene and its derivative, this two media all also exists technological deficiency, wherein the transmission usefulness of the potassium ferricyanide is poor, need to add in reagent in a large number, not only very by the interference of reducing substances in blood, as uric acid, ascorbic acid, Ammoniom-Acetate phenol etc., but also the activity of enzyme and the physical property of reagent can be affected, affect the repeatability of test result; Ferrocene and its derivative water-soluble very poor, in order to be added in reagent, need to increase production technology aspect complicacy, and due to poorly soluble, the amount of adding is very limited, limit the linear and test specification of the test of reagent, portioned product uses organic reagent to dissolve to add the physical property the activity affecting enzyme that also can affect reagent, affect the repeatability of test result.And in the market most of blood sugar electrode all receive temperature impact very large, part is carried out compensating test data to make up by machine test environment temperature, but due to the difference that temperature and the environment temperature of sample on electrode exist, cause compensation result also cannot make up the deviation of test data completely.
Utility model content
The purpose of this utility model is to overcome prior art defect, provides a kind of electric pole type blood sugar to try bar.
Concrete technical scheme of the present utility model is as follows:
Electric pole type blood sugar examination bar, comprises substrate layer, electrode layer, insulation course, layers of two-sided and hydrophilic membrane from top to bottom successively,
Electrode layer is provided with the first hematocrit than test electrode, the second hematocrit than test electrode, working electrode, contrast electrode, this hearth electrode, starting electrode and the wire that makes above-mentioned each electrode and surveying instrument form circuit to be connected;
Insulation course and layers of two-sided are equipped with and expose above-mentioned first hematocrit than test electrode, the second hematocrit than the breach of test electrode, working electrode, contrast electrode and this hearth electrode, hydrophilic membrane covers this breach; The contrast electrode exposed by above-mentioned breach and this hearth electrode are modified with the first water color ink layer containing transmitting electronic media by serigraphy, the working electrode exposed by above-mentioned breach is modified with the second water color ink layer containing transmitting electronic media and FAD-GDH by serigraphy, the medium of above-mentioned transmission electronics is that methyl methacrylate-NVP-vinyl ferrocene polymkeric substance or tri-chlorination six ammonium close ruthenium (No. CAS: 14282-91-8, purchased from sigma company);
When this electric pole type blood sugar examination bar inserts in surveying instrument, starting electrode and this hearth electrode form path, connect and start surveying instrument, closing starting electrode afterwards, between working electrode and contrast electrode, applying DC voltage, the test signal of test blood sugar sample, identical DC voltage is applied, the background signal of test blood sugar sample, by comparing of above-mentioned test signal and local signal between this hearth electrode with contrast electrode, obtain the blood glucose signal deducting local signal, primary Calculation goes out blood sugar concentration; Close working electrode, this hearth electrode and contrast electrode afterwards, the first hematocrit than test electrode and the second hematocrit than test electrode between apply high-frequency ac voltage, test the first hematocrit than test electrode and the second hematocrit than the resistance between test electrode, by calculating the hematocrit ratio of blood sugar sample, finally according to above-mentioned hematocrit ratio and environment temperature, described blood sugar concentration is compensated, draw net result.
In a preferred embodiment of the present utility model, described water color ink comprises the component of following weight portion:
The effect of above-mentioned PVP, PVA or PVPVA makes film forming after water color ink drying; Above-mentioned nano silicon or nano micro crystal cellulose are filling agent, effectively can stop haemocyte, effectively reduce the impact of hematocrit contrastive test result; Above-mentioned sodium alginate, animal gelatin or xanthans are gelling thickener, can effectively increase water color ink viscosity and in gel, not easily flow expansion after making water color ink dissolve, can effective immobilized enzyme, make it does not washed away by blood sugar sample, effectively improve the repeatability of examination bar; Above-mentioned BSA, glutamic acid or sodium succinate are protective agent, for the protection of enzyme and the medium transmitting electronics, effectively improve the stability of examination bar.
In a preferred embodiment of the present utility model, the material of described substrate layer is polyethylene terephthalate, Polyvinylchloride or polypropylene.
In a preferred embodiment of the present utility model, in described electrode layer, the material of each electrode and wire is gold, silver or graphite.
In a preferred embodiment of the present utility model, the material of described layers of two-sided is polyethylene terephthalate.
In a preferred embodiment of the present utility model, described hydrophilic membrane is through the hydrophilic treatment of one side, and its material is polycarbonate, polyethylene terephthalate or Polyvinylchloride.
In a preferred embodiment of the present utility model, described layers of two-sided is also provided with one in order to the protective seam of guard electrode layer.
In a preferred embodiment of the present utility model, the material of described protective seam is polycarbonate, polyethylene terephthalate or Polyvinylchloride.
In a preferred embodiment of the present utility model, described DC voltage is 400mV.
In a preferred embodiment of the present utility model, described high-frequency ac voltage is 30mV, 3000Hz.
The beneficial effects of the utility model are:
1, electric pole type blood sugar examination bar of the present utility model is six telegraph circuits: two electrodes use high-frequency alternating current to carry out the hematocrit ratio of test sample book, for calculation compensation coefficient; A working electrode, a contrast electrode, for testing blood glucose signal; This hearth electrode, uses the loop of contrast electrode and this hearth electrode, is getting rid of the signal disturbing of the generation of other interfering materials in sample except blood sugar; Remain a starting electrode, increase the human oriented design of instrument, cutting starts test.
2, electric pole type blood sugar examination bar of the present utility model selects FAD-GDH to replace glucose oxidase general on market, fundamentally solve the problem that glucose oxidase is subject to partial pressure of oxygen, oxygen impact, make the test result of finger tip blood identical with venous blood, and oxygen is on the impact of test result in effective reduction air, make test result more accurate, and solve glucose oxidase examination bar and to need to uncap effect phase short problem.
3, electric pole type blood sugar examination bar of the present utility model selects methyl methacrylate-NVP-vinyl ferrocene polymkeric substance or tri-chlorination six ammonium to close ruthenium as the medium transmitting electronics, replaces the potassium ferricyanide, Ferrocene and its derivative that market is conventional.The efficiency that the potassium ferricyanide transmits electronics is lower, and when trying the transmission electronic media of bar as blood sugar, the amount of needs is larger, and a large amount of potassium ferricyanides adds in ink and prints, the ductility of water color ink can be destroyed again, reduce repeatability during printing, the repeatability of impact examination bar.In atmosphere, also easily resolve into potassium ferrocyanide under illumination, examination bar background current is increased, and cause test result to occur deviation, test result is inaccurate for the potassium ferricyanide.Ferrocene water-soluble very poor, is slightly soluble in water, adds dissolve in ink very uneven to, causes the amount of the ferrocene tried in bar to occur deviation, reduce the repeatability of examination bar.Portioned product uses the derivant of ferrocene, and by chemosynthesis, ferrocene connects hydrophilic radical increases its solubleness, but effect not very large difference, can only the improvement situation of part.Portioned product uses organic solution to dissolve Ferrocene and its derivative, add in the reagent of high-speed stirred and reach dispersion object, but when reagent drying, along with the volatilization of organic reagent, easily form cavity at electrode surface, reduce the repeatability of examination bar and make examination bar larger by the impact of hematocrit ratio and temperature, and organic reagent also can reduce the stability of enzyme, make it active unstable, reduce the repeatability of examination bar.And tri-chlorination six ammonium closes the better water-soluble of ruthenium and methyl methacrylate-NVP-vinyl ferrocene polymkeric substance, transmit the usefulness of electronics higher than the potassium ferricyanide, stability is also than better, a small amount of adding in ink just effectively can transmit electronics, also reduces medium for the impact of enzyme and adds the effect phase of examination bar and the stability under trying bar exposure air or illumination.
4, the utility model adopts the mode of serigraphy to print enzyme layer, and production efficiency is high, reproducible, is applicable to producing in enormous quantities.
5, the utility model design with the addition of the water color ink of medium at contrast electrode and background electrode print, and print the water color ink that with the addition of medium and enzyme on the working electrode (s, signal value between test job electrode and contrast electrode, the signal value between this hearth electrode and contrast electrode, calculate blood glucose signal value, this signal value eliminates the impact of other interfering materials in blood, largely improves accuracy during special blood sample test blood sugar.
6, the water color ink in electric pole type blood sugar of the present utility model examination bar is as the matrix of lyase and medium; effectively can be fixed on electrode surface; form homogeneous film; effective stop haemocyte and protective enzyme, medium; can effectively reduce hematocrit than and environment temperature on the impact of test result; after compensating hematocrit ratio and environment temperature further, the accuracy of test result is by very large lifting.
Accompanying drawing explanation
Fig. 1 is the perspective exploded view of electric pole type blood sugar of the present utility model examination bar;
Fig. 2 is the structural representation of the electrode layer of electric pole type blood sugar of the present utility model examination bar.
Embodiment
Be further detailed below by way of embodiment the technical solution of the utility model and describe.
As depicted in figs. 1 and 2, a kind of electric pole type blood sugar examination bar, comprises substrate layer 11, electrode layer 12, insulation course 13, layers of two-sided 14 and hydrophilic membrane 15 from top to bottom successively,
Electrode layer 12 is provided with the first hematocrit than test electrode 121, second hematocrit than test electrode 122, working electrode 123, contrast electrode 124, this hearth electrode 125, starting electrode 126 and the wire 127 that makes above-mentioned each electrode and surveying instrument form circuit to be connected;
Insulation course 13 and layers of two-sided 14 are equipped with above-mentioned first hematocrit of exposure than the breach of test electrode 121, second hematocrit than test electrode 122, working electrode 123, contrast electrode 124 and this hearth electrode 125, and hydrophilic membrane 15 covers this breach; By serigraphy, (production efficiency is high with this hearth electrode 125 for the contrast electrode 124 exposed by above-mentioned breach, reproducible, be applicable to producing in enormous quantities) be modified with the first water color ink (not shown) containing and transmit electronic media, the working electrode 123 exposed by above-mentioned breach is modified with the second water color ink layer (not shown) containing transmitting electronic media and FAD-GDH by serigraphy, and the medium of above-mentioned transmission electronics is that methyl methacrylate-NVP-vinyl ferrocene polymkeric substance or tri-chlorination six ammonium close ruthenium; Electric pole type blood sugar examination bar of the present utility model selects FAD-GDH to replace glucose oxidase general on market, fundamentally solve the problem that glucose oxidase is subject to partial pressure of oxygen, oxygen impact, make the test result of finger tip blood identical with venous blood, and oxygen is on the impact of test result in effective reduction air, make test result more accurate, and solve glucose oxidase examination bar and to need to uncap effect phase short problem.Electric pole type blood sugar examination bar of the present utility model selects methyl methacrylate-NVP-vinyl ferrocene polymkeric substance or tri-chlorination six ammonium to close ruthenium (No. CAS: 14282-91-8, purchased from sigma company) as the medium transmitting electronics, better water-soluble, transmit the usefulness of electronics higher than the potassium ferricyanide, stability is also than better, and a small amount of adding in ink just effectively can transmit electronics.
The preparation method of above-mentioned methyl methacrylate-NVP-vinyl ferrocene polymkeric substance is as follows:
(1) NVP and ethene ferrocene 70 DEG C, in 2-isopropoxide ethanol solution, 2, the 2 ' NVP produced under the initiation catalysis of-azoisobutyronitrile-vinyl ferrocene polymkeric substance;
(2) above-mentioned NVP-vinyl ferrocene polymkeric substance is modified with methacrylic chloride in dichloromethane solution, under the catalysis of triethylamine,
(3) by the reaction product of step (2) and methyl methacrylate at 70 DEG C, 2,2 ' generate final methyl methacrylate-NVP-vinylferrocene under the initiation catalysis of-azoisobutyronitrile.
When this electric pole type blood sugar examination bar inserts in surveying instrument, starting electrode 126 and this hearth electrode 125 form path, connect and start surveying instrument, close starting electrode 126 afterwards, the DC voltage of 400mV is applied between working electrode 123 and contrast electrode 124, the test signal of test blood sugar sample, identical DC voltage is applied between this hearth electrode 125 with contrast electrode 124, the background signal of test blood sugar sample, by comparing of above-mentioned test signal and local signal, obtain the blood glucose signal deducting local signal, primary Calculation goes out blood sugar concentration; Close working electrode 123, this hearth electrode 125 and contrast electrode 124 afterwards, the first hematocrit than test electrode 121 and the second hematocrit than test electrode 122 between apply the high-frequency ac voltage of 30mV, 3000Hz, test the first hematocrit than test electrode 121 and the second hematocrit than the resistance between test electrode 122, by calculating the hematocrit ratio of blood sugar sample, finally according to above-mentioned hematocrit ratio and environment temperature, described blood sugar concentration is compensated, draw net result.
Above-mentioned water color ink comprises the component of following weight portion:
Prepare and also needed to carry out stirring, grinding, made it to dissolve dispersion, be formed with the water color ink of good ductility.
The effect of above-mentioned PVP, PVA or PVPVA makes film forming after water color ink drying; Above-mentioned nano silicon or nano micro crystal cellulose are filling agent, effectively can stop haemocyte, effectively reduce the impact of hematocrit contrastive test result; Above-mentioned sodium alginate, animal gelatin or xanthans are gelling thickener, can effectively increase water color ink viscosity and in gel, not easily flow expansion after making water color ink dissolve, can effective immobilized enzyme, make it does not washed away by blood sugar sample, effectively improve the repeatability of examination bar; Above-mentioned BSA, glutamic acid or sodium succinate are protective agent, for the protection of enzyme and the medium transmitting electronics, effectively improve the stability of examination bar.
The material of described substrate layer 11 is polyethylene terephthalate, Polyvinylchloride or polypropylene.In described electrode layer 12, the material of each electrode and wire 127 is gold, silver or graphite.The material of described layers of two-sided 14 is polyethylene terephthalate.Described hydrophilic membrane 15 is through the hydrophilic treatment of one side, and its material is polycarbonate, polyethylene terephthalate or Polyvinylchloride.Described layers of two-sided 14 is also provided with one in order to the protective seam 16 of guard electrode layer 12, the material of this protective seam 16 is polycarbonate, polyethylene terephthalate or Polyvinylchloride.
The above, be only preferred embodiment of the present utility model, therefore the scope that the utility model implements can not be limited according to this, the equivalence namely done according to the utility model the scope of the claims and description change with modify, all should still belong in scope that the utility model contains.
Claims (7)
1. electric pole type blood sugar examination bar, is characterized in that: comprise substrate layer, electrode layer, insulation course, layers of two-sided and hydrophilic membrane successively from top to bottom,
Electrode layer is provided with the first hematocrit than test electrode, the second hematocrit than test electrode, working electrode, contrast electrode, this hearth electrode, starting electrode and the wire that makes above-mentioned each electrode and surveying instrument form circuit to be connected;
Insulation course and layers of two-sided are equipped with and expose above-mentioned first hematocrit than test electrode, the second hematocrit than the breach of test electrode, working electrode, contrast electrode and this hearth electrode, hydrophilic membrane covers this breach; The contrast electrode exposed by above-mentioned breach and this hearth electrode are modified with the first water color ink layer containing transmitting electronic media by serigraphy, and the working electrode exposed by above-mentioned breach is modified with the second water color ink layer containing transmitting electronic media and FAD-GDH by serigraphy.
2. electric pole type blood sugar examination bar as claimed in claim 1, is characterized in that: the material of described substrate layer is polyethylene terephthalate, Polyvinylchloride or polypropylene.
3. electric pole type blood sugar examination bar as claimed in claim 1, is characterized in that: in described electrode layer, the material of each electrode and wire is gold, silver or graphite.
4. electric pole type blood sugar examination bar as claimed in claim 1, is characterized in that: the material of described layers of two-sided is polyethylene terephthalate.
5. electric pole type blood sugar examination bar as claimed in claim 1, is characterized in that: described hydrophilic membrane is the polycarbonate of hydrophilic treatment through one side, polyethylene terephthalate or polyvinyl chloride layer.
6. electric pole type blood sugar examination bar as claimed in claim 1, is characterized in that: described layers of two-sided is also provided with one in order to the protective seam of guard electrode layer.
7. electric pole type blood sugar examination bar as claimed in claim 6, is characterized in that: the material of described protective seam is polycarbonate, polyethylene terephthalate or Polyvinylchloride.
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| CN106770573B (en) * | 2017-01-05 | 2023-10-27 | 江苏鱼跃凯立特生物科技有限公司 | Glucose sensor |
| CN109239160A (en) * | 2018-11-13 | 2019-01-18 | 江苏鱼跃医疗设备股份有限公司 | A kind of glucose sensor of Novel free xylose interference |
| CN109856128A (en) * | 2018-12-24 | 2019-06-07 | 迪瑞医疗科技股份有限公司 | A kind of urine glucose detection test paper and preparation method thereof of ascorbic acid interference |
| CN113588935A (en) * | 2021-07-12 | 2021-11-02 | 成都云芯医联科技有限公司 | Electrochemical bar-code-free blood glucose test paper and preparation method thereof |
| CN115078508B (en) * | 2022-06-21 | 2024-01-19 | 爱森斯(江苏)生物科技有限公司 | Electrochemical biosensor and preparation method thereof |
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| CN101533007B (en) * | 2003-10-31 | 2013-01-02 | 生命扫描苏格兰有限公司 | Method of reducing the effect of direct interference current in an electrochemical test strip |
| EP3115777B1 (en) * | 2004-04-19 | 2020-01-08 | PHC Holdings Corporation | Method for measuring blood components |
| US7943022B2 (en) * | 2007-09-04 | 2011-05-17 | Lifescan, Inc. | Analyte test strip with improved reagent deposition |
| US8008037B2 (en) * | 2008-03-27 | 2011-08-30 | Roche Diagnostics Operations, Inc. | Matrix composition with alkylphenazine quaternary salt and a nitrosoaniline |
| CN102128932A (en) * | 2010-01-15 | 2011-07-20 | 泰博科技股份有限公司 | Electrochemical bio-sensing test paper, biosensor device and analyte measuring system |
| EP2596115B1 (en) * | 2010-07-23 | 2016-03-16 | Roche Diagnostics GmbH | Zwitterion buffer containing compositions and uses in electroanalytical devices and methods |
| CN102954992A (en) * | 2011-08-25 | 2013-03-06 | 崔刚 | Biosensor |
| KR101355127B1 (en) * | 2011-09-30 | 2014-01-29 | 주식회사 아이센스 | Composition of redox-reagents for electrochemical biosensor and biosensor comprising the same |
| KR101466222B1 (en) * | 2012-06-01 | 2014-12-01 | 주식회사 아이센스 | Electrochemical biosensor with improved accuracy |
| TWI513978B (en) * | 2012-06-08 | 2015-12-21 | Hmd Biomedical Inc | Test strip, detecting device and detection method |
| CN203310799U (en) * | 2013-05-23 | 2013-11-27 | 仁格（湖南）生物科技有限公司 | Blood glucose test strip with multiple working electrodes |
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Address after: 361000 Xinguang Road 332, Haicang Xinyang Industrial Zone, Xiamen City, Fujian Province Patentee after: Yingke Xinchuang (Xiamen) Science and Technology Co., Ltd. Address before: 361000 Xinguang Road 332, Haicang Xinyang Industrial Zone, Xiamen City, Fujian Province Patentee before: Yingke Xinchuang (Xiamen) Science & Technology Co., Ltd. |